Cucsa.086780 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.086780
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pectinesterase
Location	scaffold00873 : 157910 .. 159735 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTTGATTCGGAGCTGGTGGCTGCAGAAGAAAACGCCACCGTGATTAAGGTAAGGGGTGATGGAAGTGGAGATTTCAAGACAATTACTGAAGCCATTGAAAGCGTGCCAGCGTGTAACACGAAACGTGTAGTGATTTGGATCGGAGGAGGAGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGCCTTTCGTTACTCTTTATGGATCTCCTAATAATATGCCTAATTTGACCTTCGATGGAGATGCTAGCAAATACGGCACCGTTTATAGTGCTACCTTGACGGTTGAAGCTGATTATTTCACTGCAGCTAATCTCATCATTGAGGTATAATTTGTTTATTAAATATGTTTTAAGGTTAAATAATTAACAAAATTATTTCTATAACTCTAAGAAATTAGAATTTAGTTCTTGTAGTTGGATAGAAGAGATACTATTATTTATCACGTTTTACCAAATATAACTAATATTGACTAGTCAATTTGTAAAGTTTTTGAAATTCTAAGTTTTCCCTTTAGCGCACGTGTGTCCATCATTAATAGCAACCATAGATAGTCACACTTAATAACATGTTTTTCAAATTGATAGCTAACCCTAATAGCAGTTATATCGGTGATATTCATTGATAGCAACAGAAAACACATTTCTCAAATGAAGTGATATTCATTGATAGCAACTGATAGTACGTTTTTCAAATTGATCAAAGCTACCACTAATAGCAGTTATCAGTTAGAGCAATCGATAGTAGTAAAAAaTGAAAGTTATCACTAATACCAATTACTTGTTTTTGTGGTAGGCGTGAGCTTTTTAATATTTTATCAATTGTGAAGTTATATATGCAGTTACTTTATATATATATATATATTATTTCATTATGATGAATAGGAAAATGAGAAATTATCAATAACTAAACTTAAATATAACCCTTTTATAAAAATCATATTTGTTGAAAAACAATACACATGAACAGAATTCATCGCCAAGGCCAGATGGGAAAAGAAAAGGAGAGCAAGCATTAGCAGCAAGAATTTGGGGAAATAAAGTCGCAATTTATAATTGCAAATTCATTGGATTTCAAGATACCCTTTGCGACGATCGGGGTTTACATCTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAGGAGGAACTTCCCTTTATTTGGTATAATTTAATTATATTATAATTACTGTTCTTGTATAGATATTTAAAACGAATATTATAATTTTAGTTTTGAGAAAATGATTCATTACAGAACACTCAGTTGGATGTGGTGGTCGATGGAGGATTGGGAGTGATAGCAGCCCATTCAAGAGAACAAGAATCTGAGTGCAGTGGATTCTCATTTGTGCATTGTAGCATTACAGGAACTGGCGGAAGAAACACTTATTTGGGAAGAGCTTGGAGGCCAAGATCTAGAGTTGTTTTTGCCTACACTACAATGGCTGATATTATTCATCCCAAAGGCTGGAACGACATGGACAATTTTGGTTACGACAAGTACGTAACAGCTTTCACATTCTTTTTACTTCATTTCTTTTCTAAAGATTAAAATTAGGACAAATATGATTTAAAGTACTTGGGGATTGTTACATTGAATGATACGTACTTTTCCTTTTTCTACTTTTACAGAACGGTTTCGTTCGGAGAATACAAGTGTTCGGGGCTGGGTTCGAACTTTAGCAAGCGTGTGCAATACGGCAAGCAATTGAGTGATGAAAAAGTGCAGCAGTTTGTTAGTCTTGAATACTTGAAGAGTGATACATGGCTACTTCCTCCTCCCAATCACGAAGATTTAGAAAATTAG

mRNA sequence

ATGAAGCTTGATTCGGAGCTGGTGGCTGCAGAAGAAAACGCCACCGTGATTAAGGTAAGGGGTGATGGAAGTGGAGATTTCAAGACAATTACTGAAGCCATTGAAAGCGTGCCAGCGTGTAACACGAAACGTGTAGTGATTTGGATCGGAGGAGGAGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGCCTTTCGTTACTCTTTATGGATCTCCTAATAATATGCCTAATTTGACCTTCGATGGAGATGCTAGCAAATACGGCACCGTTTATAGTGCTACCTTGACGGTTGAAGCTGATTATTTCACTGCAGCTAATCTCATCATTGAGAATTCATCGCCAAGGCCAGATGGGAAAAGAAAAGGAGAGCAAGCATTAGCAGCAAGAATTTGGGGAAATAAAGTCGCAATTTATAATTGCAAATTCATTGGATTTCAAGATACCCTTTGCGACGATCGGGGTTTACATCTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAGGAGGAACTTCCCTTTATTTGAACACTCAGTTGGATGTGGTGGTCGATGGAGGATTGGGAGTGATAGCAGCCCATTCAAGAGAACAAGAATCTGAGTGCAGTGGATTCTCATTTGTGCATTGTAGCATTACAGGAACTGGCGGAAGAAACACTTATTTGGGAAGAGCTTGGAGGCCAAGATCTAGAGTTGTTTTTGCCTACACTACAATGGCTGATATTATTCATCCCAAAGGCTGGAACGACATGGACAATTTTGGTTACGACAAAACGGTTTCGTTCGGAGAATACAAGTGTTCGGGGCTGGGTTCGAACTTTAGCAAGCGTGTGCAATACGGCAAGCAATTGAGTGATGAAAAAGTGCAGCAGTTTGTTAGTCTTGAATACTTGAAGAGTGATACATGGCTACTTCCTCCTCCCAATCACGAAGATTTAGAAAATTAG

Coding sequence (CDS)

ATGAAGCTTGATTCGGAGCTGGTGGCTGCAGAAGAAAACGCCACCGTGATTAAGGTAAGGGGTGATGGAAGTGGAGATTTCAAGACAATTACTGAAGCCATTGAAAGCGTGCCAGCGTGTAACACGAAACGTGTAGTGATTTGGATCGGAGGAGGAGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGCCTTTCGTTACTCTTTATGGATCTCCTAATAATATGCCTAATTTGACCTTCGATGGAGATGCTAGCAAATACGGCACCGTTTATAGTGCTACCTTGACGGTTGAAGCTGATTATTTCACTGCAGCTAATCTCATCATTGAGAATTCATCGCCAAGGCCAGATGGGAAAAGAAAAGGAGAGCAAGCATTAGCAGCAAGAATTTGGGGAAATAAAGTCGCAATTTATAATTGCAAATTCATTGGATTTCAAGATACCCTTTGCGACGATCGGGGTTTACATCTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAGGAGGAACTTCCCTTTATTTGAACACTCAGTTGGATGTGGTGGTCGATGGAGGATTGGGAGTGATAGCAGCCCATTCAAGAGAACAAGAATCTGAGTGCAGTGGATTCTCATTTGTGCATTGTAGCATTACAGGAACTGGCGGAAGAAACACTTATTTGGGAAGAGCTTGGAGGCCAAGATCTAGAGTTGTTTTTGCCTACACTACAATGGCTGATATTATTCATCCCAAAGGCTGGAACGACATGGACAATTTTGGTTACGACAAAACGGTTTCGTTCGGAGAATACAAGTGTTCGGGGCTGGGTTCGAACTTTAGCAAGCGTGTGCAATACGGCAAGCAATTGAGTGATGAAAAAGTGCAGCAGTTTGTTAGTCTTGAATACTTGAAGAGTGATACATGGCTACTTCCTCCTCCCAATCACGAAGATTTAGAAAATTAG

Protein sequence

MKLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYLKSDTWLLPPPNHEDLEN*

Homology

[Close]

BLAST of Cucsa.086780 vs. Swiss-Prot
Match: PME63_ARATH (Putative pectinesterase 63 OS=Arabidopsis thaliana GN=PME63 PE=3 SV=2)

HSP 1 Score: 368.2 bits (944), Expect = 8.9e-101
Identity = 176/295 (59.66%), Postives = 224/295 (75.93%), Query Frame = 1


Query: 19  VRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPN 78
           V+ +G G FKTITEAI SV A NT+RV+I IG GVYKEK+ IDR+KPF+TLYG PN MP 
Sbjct: 45  VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAMPV 104

Query: 79  LTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAI 138
           LTFDG A++YGTV SATL V +DYF A N+I++NS+P PDGKRKG QAL+ RI GNK A 
Sbjct: 105 LTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAAF 164

Query: 139 YNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNTQLDVVVDGGLGVIAAH 198
           YNCKF G+QDT+CDD G H +KDC+I+GT DFIFG G SLYL TQL+VV D G+ VI AH
Sbjct: 165 YNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD-GIRVITAH 224

Query: 199 SREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPKGWNDMDNF 258
           + +  +E SG+SFVHC +TGT G   YLGR+W    +VV+AYT M+ +++P GW +    
Sbjct: 225 AGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENREA 284

Query: 259 GYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYLKSDTWLLPPPN 314
           G DKTV +GEYKC+G GS+  KRV+Y + + D + + F+SL Y++  +WLLPPP+
Sbjct: 285 GRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPPS 337

[Close]

BLAST of Cucsa.086780 vs. Swiss-Prot
Match: PPME1_ARATH (Pectinesterase PPME1 OS=Arabidopsis thaliana GN=PPME1 PE=1 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.4e-100
Identity = 176/310 (56.77%), Postives = 228/310 (73.55%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           LD  LVAAE    +I V   G G+FKT+T+AI+SVPA NTKRV+I +  G YKEK+ IDR
Sbjct: 53  LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDR 112

Query: 63  NKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRK 122
           NKPF+TL G PN MP +T+DG A+KYGTV SA+L + +DYF A N++++N++P PDGK K
Sbjct: 113 NKPFITLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTK 172

Query: 123 GEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNT 182
           G QAL+ RI GN  A YNCKF GFQDT+CDD G H +KDC+++GT DFIFG GTS+YL T
Sbjct: 173 GAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGT 232

Query: 183 QLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTT 242
           QL VV D G+ VIAAH+ +   E SG+SFVHC +TGTGG   YLGRAW    +VV+AYT 
Sbjct: 233 QLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYTE 292

Query: 243 MADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYL 302
           M  +++P GW +     +DKTV +GEYKCSG GS+ +KRV + + + D++  +F+SL Y+
Sbjct: 293 MTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYI 352

Query: 303 KSDTWLLPPP 313
           +   WLLPPP
Sbjct: 353 QGSKWLLPPP 359

[Close]

BLAST of Cucsa.086780 vs. Swiss-Prot
Match: PME48_ARATH (Probable pectinesterase 48 OS=Arabidopsis thaliana GN=PME48 PE=2 SV=2)

HSP 1 Score: 363.6 bits (932), Expect = 2.2e-99
Identity = 175/310 (56.45%), Postives = 227/310 (73.23%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           LD  LVAAE    +I V   G G+FKT+T+AI+SVPA NTKRV+I +  G Y+EK+ IDR
Sbjct: 53  LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDR 112

Query: 63  NKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRK 122
           NKPF+TL G PN MP +T+DG A+KYGTV SA+L + +DYF A N++++N++P PDGK K
Sbjct: 113 NKPFITLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTK 172

Query: 123 GEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNT 182
           G QAL+ RI GN  A YNCKF GFQDT+CDD G H +KDC+++GT DFIFG GTS+YL T
Sbjct: 173 GAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGT 232

Query: 183 QLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTT 242
           QL VV D G+ VIAAH+ +   E SG+SFVHC +TGTGG   YLGRAW    +VV+AYT 
Sbjct: 233 QLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYTE 292

Query: 243 MADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYL 302
           M  +++P GW +     +DKTV +GEYKCSG GS+ +KRV + + + D++   F+SL Y+
Sbjct: 293 MTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYI 352

Query: 303 KSDTWLLPPP 313
           +   WLLPPP
Sbjct: 353 QGSKWLLPPP 359

[Close]

BLAST of Cucsa.086780 vs. Swiss-Prot
Match: AL11B_OLEEU (Pectinesterase 2 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.0e-88
Identity = 166/310 (53.55%), Postives = 216/310 (69.68%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           +D  LV AE  A VIK++ DGSGDFK+I EAI+S+P  NTKRV++    G Y EK+KI  
Sbjct: 53  MDPALVTAEGQAKVIKLKSDGSGDFKSINEAIKSIPDDNTKRVILSFSPGNYSEKVKIGM 112

Query: 63  NKPFVTLYGS-PNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 122
            K ++T YG  PNNMP L F G A++YGTV SATL VE++YF+A NL I NS+PRPDGKR
Sbjct: 113 YKHYITFYGEDPNNMPILVFGGTAAEYGTVDSATLIVESNYFSAVNLKIVNSAPRPDGKR 172

Query: 123 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLN 182
            G QA A RI G+K + YN K  GFQDTLCDD+G H YKDC+I+GTVDFIFG G S++LN
Sbjct: 173 VGAQAAALRISGDKASFYNVKIYGFQDTLCDDKGKHFYKDCYIEGTVDFIFGSGKSIFLN 232

Query: 183 TQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 242
           T+L  V      +I A +R+ ESE +G+ FV+C +TG G    +LGR+W P ++VVFAYT
Sbjct: 233 TELHAVPGDQPAIITAQARKTESEDTGYYFVNCRVTGGG---AFLGRSWMPAAKVVFAYT 292

Query: 243 TMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEY 302
            M D IHP+GW  +    ++ TV F EY   G G+N  KR ++ K+LSD + +Q +SL  
Sbjct: 293 EMGDAIHPEGW-ILVKPEHESTVRFPEYNNKGPGANMEKRAKFVKRLSDAEAKQSISLGS 352

Query: 303 LKSDTWLLPP 312
           +++  WLLPP
Sbjct: 353 IEASKWLLPP 358

[Close]

BLAST of Cucsa.086780 vs. Swiss-Prot
Match: AL11A_OLEEU (Pectinesterase 1 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.3e-88
Identity = 164/310 (52.90%), Postives = 216/310 (69.68%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           +D  LV AE    VIK++ DGSGDFK+I EAI+S+P  NTKRV++ +  G Y EK+KI  
Sbjct: 53  MDPALVTAEGQTKVIKLKSDGSGDFKSINEAIKSIPDDNTKRVILSLAPGNYSEKVKIGM 112

Query: 63  NKPFVTLYGS-PNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 122
            K ++T YG  PNNMP L F G A++YGTV SATL VE++YF+A NL I NS+PRPDGKR
Sbjct: 113 YKHYITFYGEDPNNMPILVFGGTAAEYGTVDSATLIVESNYFSAVNLKIVNSAPRPDGKR 172

Query: 123 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLN 182
            G QA A RI G+K + YN K  GFQDTLCDD+G H YKDC+I+GTVDFIFG G S++LN
Sbjct: 173 VGAQAAALRISGDKASFYNVKIYGFQDTLCDDKGKHFYKDCYIEGTVDFIFGSGKSIFLN 232

Query: 183 TQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 242
           T+L  V      +I A +R+ +SE +G+ FV+C +TG G    +LGR+W P ++VVFAYT
Sbjct: 233 TELHAVPGDQPAIITAQARKTDSEDTGYYFVNCRVTGGG---AFLGRSWMPAAKVVFAYT 292

Query: 243 TMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEY 302
            M D IHP+GW  +    ++ TV F EY   G G+N  KR ++ K+LSD + +Q +SL  
Sbjct: 293 EMVDAIHPEGW-ILVKPEHESTVRFSEYNNKGPGANMEKRAKFVKRLSDAEAKQSISLGS 352

Query: 303 LKSDTWLLPP 312
           +++  WLLPP
Sbjct: 353 IEASKWLLPP 358

[Close]

BLAST of Cucsa.086780 vs. TrEMBL
Match: A0A0A0KEY2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G428540 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 1.6e-186
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 1


Query: 1   MKLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKI 60
           MKLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKI
Sbjct: 55  MKLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKI 114

Query: 61  DRNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK 120
           DRNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK
Sbjct: 115 DRNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK 174

Query: 121 RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL 180
           RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL
Sbjct: 175 RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL 234

Query: 181 NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY 240
           NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY
Sbjct: 235 NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY 294

Query: 241 TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE 300
           TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE
Sbjct: 295 TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE 354

Query: 301 YLKSDTWLLPPPNHEDLEN 320
           YLKSDTWLLPPPNHEDLEN
Sbjct: 355 YLKSDTWLLPPPNHEDLEN 373

[Close]

BLAST of Cucsa.086780 vs. TrEMBL
Match: A0A0A0KEH2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G428030 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 3.6e-141
Identity = 248/313 (79.23%), Postives = 272/313 (86.90%), Query Frame = 1


Query: 2   KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 61
           +LD  L  AEENATVIKV  DG+G+FKT+TEAI SVPA N KRVVIWIG GVYKEKLKID
Sbjct: 55  ELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKID 114

Query: 62  RNKPFVTLYGS-PNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK 121
           RNKPFVTLYGS P NMP LTFDGDA+KYGTVYSATL VEADYFTAANLIIENSSPRPDG 
Sbjct: 115 RNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGV 174

Query: 122 RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL 181
           RKG QALAAR  G K AIYNCKF+GFQDTLCDD GLH YKDCFIQGTVDFIFG GTSLYL
Sbjct: 175 RKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYL 234

Query: 182 NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY 241
           NTQLDV  DGGL VI AHSREQE++ SG+SFVHCSITGTGG+NTYLGRAW PRSRVVFAY
Sbjct: 235 NTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAY 294

Query: 242 TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE 301
           TT+ADIIHP+GWNDM + G+DKTV FGEYKCSG G+  + RV YGKQL+D +V+ F+ LE
Sbjct: 295 TTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLE 354

Query: 302 YLKSDTWLLPPPN 314
           Y++S+ WLLPPP+
Sbjct: 355 YVQSEKWLLPPPS 367

[Close]

BLAST of Cucsa.086780 vs. TrEMBL
Match: A0A0A0KJZ4_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G428040 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.5e-126
Identity = 223/312 (71.47%), Postives = 262/312 (83.97%), Query Frame = 1


Query: 2   KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 61
           +LD  +VAAE N T++KV GDG+GDFKTITEAI SVP  N  RVVIWIG GVYKEKL I+
Sbjct: 27  ELDPAVVAAEGNVTIVKVMGDGTGDFKTITEAIASVPVNNKNRVVIWIGEGVYKEKLTIE 86

Query: 62  RNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 121
           +NKPF+TL G+P N+P L+FDG ASKYGTVYSATL VEADYF AANLIIEN+SPRP+G R
Sbjct: 87  KNKPFITLCGTPKNVPTLSFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG-R 146

Query: 122 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLN 181
           K  QALAAR  G K A YNCKF+GFQDTLCDD GLHLYKDCFIQGTVDF+FG GTSLYLN
Sbjct: 147 KEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLYLN 206

Query: 182 TQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 241
           T+L+VV +G   VI AHSREQE++ SG+SFVHCSITG  G++TYLGRAW PRSRV+FAYT
Sbjct: 207 TELNVVGEGQFAVITAHSREQEADASGYSFVHCSITG-NGKDTYLGRAWMPRSRVIFAYT 266

Query: 242 TMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEY 301
           +M DIIHP+GWNDM + G+DKTV FGEYKCSG G+  + RV YGKQL++ +V+ ++SLE+
Sbjct: 267 SMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEAEVKPYLSLEF 326

Query: 302 LKSDTWLLPPPN 314
           ++S  WLLPPPN
Sbjct: 327 VQSAKWLLPPPN 336

[Close]

BLAST of Cucsa.086780 vs. TrEMBL
Match: A0A0A0KI78_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G428290 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 9.5e-126
Identity = 221/312 (70.83%), Postives = 261/312 (83.65%), Query Frame = 1


Query: 2   KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 61
           +LD  +VAAE N T++KV  DG+GDFKT+TEAI SVP  N  RVVIWIG GVYKEKL I+
Sbjct: 56  ELDPAVVAAEGNVTIVKVMSDGTGDFKTVTEAIASVPVNNKNRVVIWIGEGVYKEKLTIE 115

Query: 62  RNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 121
           +NKPF+TL G+P N+P LTFDG ASKYGTVYSATL VEADYF AANLIIEN+SPRP+G R
Sbjct: 116 KNKPFITLCGTPKNVPTLTFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG-R 175

Query: 122 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLN 181
           K  QALAAR  G K A YNCKF+GFQDTLCDD GLHLYKDCFIQGTVDF+FG GTSLYLN
Sbjct: 176 KEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLYLN 235

Query: 182 TQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 241
           T+L+VV +G   VI AHSREQE++ SG+SFVHCSITG  G++T+LGRAW PRSRV+FAYT
Sbjct: 236 TELNVVGEGQFAVITAHSREQEADASGYSFVHCSITG-NGKDTFLGRAWMPRSRVIFAYT 295

Query: 242 TMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEY 301
           +M DIIHP+GWNDM + G+DKTV FGEYKCSG G+  + RV YGKQL++ +V+ ++SLE+
Sbjct: 296 SMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEAEVKPYLSLEF 355

Query: 302 LKSDTWLLPPPN 314
           ++S  WLLPPPN
Sbjct: 356 VQSAKWLLPPPN 365

[Close]

BLAST of Cucsa.086780 vs. TrEMBL
Match: M5XK08_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa007533mg PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.1e-109
Identity = 195/311 (62.70%), Postives = 238/311 (76.53%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           LD  L  AE +  VIKV  +GSG FKTIT+A+ S+PA NTKRV+++IGGG Y EK+ I R
Sbjct: 52  LDPTLAKAEASQKVIKVMKNGSGQFKTITDAVNSIPAGNTKRVIVYIGGGEYNEKITIPR 111

Query: 63  NKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRK 122
           NKPFVT YGSP NMP LTF G + KYGTV SAT+  E+DYF AAN+II+NSSPRPDGKR 
Sbjct: 112 NKPFVTFYGSPTNMPTLTFAGTSQKYGTVNSATVIAESDYFVAANVIIKNSSPRPDGKRV 171

Query: 123 GEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNT 182
           G QA+A R+ GNK A+YNCK IGFQDTLCDDRG HL+KDCFI+GTVDFI+G G SLYLNT
Sbjct: 172 GAQAVALRVSGNKSALYNCKLIGFQDTLCDDRGNHLFKDCFIEGTVDFIWGSGKSLYLNT 231

Query: 183 QLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTT 242
           +L V+ D GL VI A +R+  SE +G+SFVHC ITGTG   TYLGRAWR    VV+AYT+
Sbjct: 232 ELHVLGDNGLTVITAQARDSASEDTGYSFVHCKITGTGS-GTYLGRAWRTNPMVVYAYTS 291

Query: 243 MADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYL 302
           M  +I+P GW+D ++   D TV +GEYKCSG GS+   R +Y K L+DE+++ F+SL Y+
Sbjct: 292 MTKVINPAGWSDNNHPERDSTVFYGEYKCSGPGSSAVGREKYTKHLTDEQIKPFLSLGYI 351

Query: 303 KSDTWLLPPPN 314
           +   WLLPPPN
Sbjct: 352 QGSKWLLPPPN 361

[Close]

BLAST of Cucsa.086780 vs. TAIR10
Match: AT5G61680.1 (AT5G61680.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 368.2 bits (944), Expect = 5.0e-102
Identity = 176/295 (59.66%), Postives = 224/295 (75.93%), Query Frame = 1


Query: 19  VRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPN 78
           V+ +G G FKTITEAI SV A NT+RV+I IG GVYKEK+ IDR+KPF+TLYG PN MP 
Sbjct: 45  VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAMPV 104

Query: 79  LTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAI 138
           LTFDG A++YGTV SATL V +DYF A N+I++NS+P PDGKRKG QAL+ RI GNK A 
Sbjct: 105 LTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAAF 164

Query: 139 YNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNTQLDVVVDGGLGVIAAH 198
           YNCKF G+QDT+CDD G H +KDC+I+GT DFIFG G SLYL TQL+VV D G+ VI AH
Sbjct: 165 YNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD-GIRVITAH 224

Query: 199 SREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPKGWNDMDNF 258
           + +  +E SG+SFVHC +TGT G   YLGR+W    +VV+AYT M+ +++P GW +    
Sbjct: 225 AGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENREA 284

Query: 259 GYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYLKSDTWLLPPPN 314
           G DKTV +GEYKC+G GS+  KRV+Y + + D + + F+SL Y++  +WLLPPP+
Sbjct: 285 GRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPPS 337

[Close]

BLAST of Cucsa.086780 vs. TAIR10
Match: AT1G69940.1 (AT1G69940.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 366.3 bits (939), Expect = 1.9e-101
Identity = 176/310 (56.77%), Postives = 228/310 (73.55%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           LD  LVAAE    +I V   G G+FKT+T+AI+SVPA NTKRV+I +  G YKEK+ IDR
Sbjct: 53  LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDR 112

Query: 63  NKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRK 122
           NKPF+TL G PN MP +T+DG A+KYGTV SA+L + +DYF A N++++N++P PDGK K
Sbjct: 113 NKPFITLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTK 172

Query: 123 GEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNT 182
           G QAL+ RI GN  A YNCKF GFQDT+CDD G H +KDC+++GT DFIFG GTS+YL T
Sbjct: 173 GAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGT 232

Query: 183 QLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTT 242
           QL VV D G+ VIAAH+ +   E SG+SFVHC +TGTGG   YLGRAW    +VV+AYT 
Sbjct: 233 QLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYTE 292

Query: 243 MADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYL 302
           M  +++P GW +     +DKTV +GEYKCSG GS+ +KRV + + + D++  +F+SL Y+
Sbjct: 293 MTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYI 352

Query: 303 KSDTWLLPPP 313
           +   WLLPPP
Sbjct: 353 QGSKWLLPPP 359

[Close]

BLAST of Cucsa.086780 vs. TAIR10
Match: AT5G07410.1 (AT5G07410.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 363.6 bits (932), Expect = 1.2e-100
Identity = 175/310 (56.45%), Postives = 227/310 (73.23%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           LD  LVAAE    +I V   G G+FKT+T+AI+SVPA NTKRV+I +  G Y+EK+ IDR
Sbjct: 53  LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDR 112

Query: 63  NKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRK 122
           NKPF+TL G PN MP +T+DG A+KYGTV SA+L + +DYF A N++++N++P PDGK K
Sbjct: 113 NKPFITLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTK 172

Query: 123 GEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNT 182
           G QAL+ RI GN  A YNCKF GFQDT+CDD G H +KDC+++GT DFIFG GTS+YL T
Sbjct: 173 GAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGT 232

Query: 183 QLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTT 242
           QL VV D G+ VIAAH+ +   E SG+SFVHC +TGTGG   YLGRAW    +VV+AYT 
Sbjct: 233 QLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYTE 292

Query: 243 MADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYL 302
           M  +++P GW +     +DKTV +GEYKCSG GS+ +KRV + + + D++   F+SL Y+
Sbjct: 293 MTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYI 352

Query: 303 KSDTWLLPPP 313
           +   WLLPPP
Sbjct: 353 QGSKWLLPPP 359

[Close]

BLAST of Cucsa.086780 vs. TAIR10
Match: AT5G07420.1 (AT5G07420.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 326.2 bits (835), Expect = 2.2e-89
Identity = 165/310 (53.23%), Postives = 214/310 (69.03%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           LD EL AAE +  VI V  +G GDFKTI  AI+S+P  N  RV+I +  G+Y EK+ +D 
Sbjct: 53  LDPELEAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDV 112

Query: 63  NKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRK 122
            +P+VTL G P    NLT+ G A+KYGTV SATL V A  F AANL I N+SP P    +
Sbjct: 113 GRPYVTLLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPGTQ 172

Query: 123 GEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNT 182
           G QALA RI G+K A YNC+F GFQDTLCDDRG H +K+C+I+GT DFIFG G SLYL T
Sbjct: 173 G-QALAMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTT 232

Query: 183 QLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTT 242
           QL  V D GL VIAAH+R+  +E +G+SFVHC +TG  G   YLGRAW    +VV++YT 
Sbjct: 233 QLHAVGD-GLRVIAAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRAWMSHPKVVYSYTE 292

Query: 243 MADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYL 302
           M+ +++P GW +     +DKTV +GEY C+G GS+ +KRV + + + +++  QF++L Y+
Sbjct: 293 MSSVVNPSGWQENRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYI 352

Query: 303 KSDTWLLPPP 313
           K   WLLPPP
Sbjct: 353 KGSKWLLPPP 359

[Close]

BLAST of Cucsa.086780 vs. TAIR10
Match: AT5G07430.1 (AT5G07430.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 322.8 bits (826), Expect = 2.4e-88
Identity = 162/312 (51.92%), Postives = 211/312 (67.63%), Query Frame = 1


Query: 3   LDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDR 62
           LD  L AAE    +I V   G  +FKT+ EAI+S+P  N  RV+I +  GVY EK+ ID 
Sbjct: 53  LDPALEAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDI 112

Query: 63  NKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRK 122
            +PF+TL G P     LT+ G A++YGTV SATL V A+YF AA+L I+N++P P    +
Sbjct: 113 ARPFITLLGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPGSQ 172

Query: 123 GEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNT 182
           G QALA RI  +K A Y+C+F GFQDTLCDD+G H +KDC+I+GT DFIFG G SLYLNT
Sbjct: 173 G-QALAMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNT 232

Query: 183 QLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTT 242
           QL  V D GL VI A  R+  +E +G++FVHC +TGT G   YLGR+W    +VV+A+T 
Sbjct: 233 QLHAVGD-GLRVITAQGRQSATEQNGYTFVHCKVTGT-GTGIYLGRSWMSHPKVVYAFTE 292

Query: 243 MADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYL 302
           M  +++P GW +  N GYDKTV +GEYKC G GS+  KRV Y + +   +V  F++L Y+
Sbjct: 293 MTSVVNPSGWRENLNRGYDKTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYI 352

Query: 303 KSDTWLLPPPNH 315
           K  TWLLPPP +
Sbjct: 353 KGSTWLLPPPKY 361

[Close]

BLAST of Cucsa.086780 vs. NCBI nr
Match: gi|778722333|ref|XP_004140274.2| (PREDICTED: putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 659.8 bits (1701), Expect = 2.4e-186
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 1


Query: 1   MKLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKI 60
           MKLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKI
Sbjct: 55  MKLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKI 114

Query: 61  DRNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK 120
           DRNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK
Sbjct: 115 DRNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK 174

Query: 121 RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL 180
           RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL
Sbjct: 175 RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL 234

Query: 181 NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY 240
           NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY
Sbjct: 235 NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY 294

Query: 241 TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE 300
           TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE
Sbjct: 295 TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE 354

Query: 301 YLKSDTWLLPPPNHEDLEN 320
           YLKSDTWLLPPPNHEDLEN
Sbjct: 355 YLKSDTWLLPPPNHEDLEN 373

[Close]

BLAST of Cucsa.086780 vs. NCBI nr
Match: gi|659097432|ref|XP_008449623.1| (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 557.0 bits (1434), Expect = 2.2e-155
Identity = 272/314 (86.62%), Postives = 290/314 (92.36%), Query Frame = 1


Query: 2   KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 61
           +LD ELVAAEE+ATVIKVRGDGSGDFKTI EAIESVP  NTKR+VIWIGGGVYKEKLKID
Sbjct: 55  ELDPELVAAEESATVIKVRGDGSGDFKTIAEAIESVPIGNTKRIVIWIGGGVYKEKLKID 114

Query: 62  RNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 121
           RNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATL VEADYFTAANL+IENSSPRPDG R
Sbjct: 115 RNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLIVEADYFTAANLVIENSSPRPDGVR 174

Query: 122 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLN 181
           KG QALAARI GNK AIYNCKFIGFQDTLCDD GLHLYKDCFIQGTVDFIFG GTSLYLN
Sbjct: 175 KGAQALAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLN 234

Query: 182 TQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 241
           TQLDVV +GGLGVIAAHSR+QES+ SG+SFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT
Sbjct: 235 TQLDVVGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 294

Query: 242 TMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEY 301
           TMADIIHP+GWNDM++FGYDKTV FGEYKC G GS  S+RV+Y KQLS E+V+ FVSL Y
Sbjct: 295 TMADIIHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVSLGY 354

Query: 302 LKSDTWLLPPPNHE 316
           ++S+ WLLPPPN E
Sbjct: 355 VQSEKWLLPPPNPE 368

[Close]

BLAST of Cucsa.086780 vs. NCBI nr
Match: gi|659097434|ref|XP_008449624.1| (PREDICTED: pectinesterase PPME1-like [Cucumis melo])

HSP 1 Score: 511.1 bits (1315), Expect = 1.4e-141
Identity = 248/312 (79.49%), Postives = 271/312 (86.86%), Query Frame = 1


Query: 2   KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 61
           +LD  LVAAEENAT+IKV  DG+GDFKTITEAI SVPA N KRV+I IG G+YKEKLKID
Sbjct: 54  ELDPALVAAEENATIIKVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKID 113

Query: 62  RNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 121
           RNKPFVTLYGSP  MP LTFDGDA+K+GTVYSATL VEADYFTAANL+IENSSPRPDG R
Sbjct: 114 RNKPFVTLYGSPKGMPKLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVR 173

Query: 122 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLN 181
           KG QALAAR  G K AIYNCKF GFQDTLCDD GLH YKDCFIQGTVDFIFG GTSLY+N
Sbjct: 174 KGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVN 233

Query: 182 TQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 241
           TQLDV  DGGL VI AHSREQES+ SG+SFVHCSITGTGG+NT+LGRAW PRSRVVFAYT
Sbjct: 234 TQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYT 293

Query: 242 TMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEY 301
           TMADIIHP+GWNDM + G+DKTV FGEYKCSG GS  ++RV YGKQLSD +V  F+SL Y
Sbjct: 294 TMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAY 353

Query: 302 LKSDTWLLPPPN 314
           ++S+ WLLPPPN
Sbjct: 354 VQSEKWLLPPPN 365

[Close]

BLAST of Cucsa.086780 vs. NCBI nr
Match: gi|778716816|ref|XP_011657599.1| (PREDICTED: putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 509.2 bits (1310), Expect = 5.2e-141
Identity = 248/313 (79.23%), Postives = 272/313 (86.90%), Query Frame = 1


Query: 2   KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 61
           +LD  L  AEENATVIKV  DG+G+FKT+TEAI SVPA N KRVVIWIG GVYKEKLKID
Sbjct: 55  ELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKID 114

Query: 62  RNKPFVTLYGS-PNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK 121
           RNKPFVTLYGS P NMP LTFDGDA+KYGTVYSATL VEADYFTAANLIIENSSPRPDG 
Sbjct: 115 RNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGV 174

Query: 122 RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL 181
           RKG QALAAR  G K AIYNCKF+GFQDTLCDD GLH YKDCFIQGTVDFIFG GTSLYL
Sbjct: 175 RKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYL 234

Query: 182 NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY 241
           NTQLDV  DGGL VI AHSREQE++ SG+SFVHCSITGTGG+NTYLGRAW PRSRVVFAY
Sbjct: 235 NTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAY 294

Query: 242 TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE 301
           TT+ADIIHP+GWNDM + G+DKTV FGEYKCSG G+  + RV YGKQL+D +V+ F+ LE
Sbjct: 295 TTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLE 354

Query: 302 YLKSDTWLLPPPN 314
           Y++S+ WLLPPP+
Sbjct: 355 YVQSEKWLLPPPS 367

[Close]

BLAST of Cucsa.086780 vs. NCBI nr
Match: gi|778716819|ref|XP_004140272.2| (PREDICTED: putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 460.7 bits (1184), Expect = 2.1e-126
Identity = 223/312 (71.47%), Postives = 262/312 (83.97%), Query Frame = 1


Query: 2   KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 61
           +LD  +VAAE N T++KV GDG+GDFKTITEAI SVP  N  RVVIWIG GVYKEKL I+
Sbjct: 56  ELDPAVVAAEGNVTIVKVMGDGTGDFKTITEAIASVPVNNKNRVVIWIGEGVYKEKLTIE 115

Query: 62  RNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 121
           +NKPF+TL G+P N+P L+FDG ASKYGTVYSATL VEADYF AANLIIEN+SPRP+G R
Sbjct: 116 KNKPFITLCGTPKNVPTLSFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG-R 175

Query: 122 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLN 181
           K  QALAAR  G K A YNCKF+GFQDTLCDD GLHLYKDCFIQGTVDF+FG GTSLYLN
Sbjct: 176 KEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLYLN 235

Query: 182 TQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 241
           T+L+VV +G   VI AHSREQE++ SG+SFVHCSITG  G++TYLGRAW PRSRV+FAYT
Sbjct: 236 TELNVVGEGQFAVITAHSREQEADASGYSFVHCSITG-NGKDTYLGRAWMPRSRVIFAYT 295

Query: 242 TMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEY 301
           +M DIIHP+GWNDM + G+DKTV FGEYKCSG G+  + RV YGKQL++ +V+ ++SLE+
Sbjct: 296 SMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEAEVKPYLSLEF 355

Query: 302 LKSDTWLLPPPN 314
           ++S  WLLPPPN
Sbjct: 356 VQSAKWLLPPPN 365

The following BLAST results are available for this feature:

BLAST of Cucsa.086780 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME63_ARATH	8.9e-101	59.66	Putative pectinesterase 63 OS=Arabidopsis thaliana GN=PME63 PE=3 SV=2	[more]
PPME1_ARATH	3.4e-100	56.77	Pectinesterase PPME1 OS=Arabidopsis thaliana GN=PPME1 PE=1 SV=1	[more]
PME48_ARATH	2.2e-99	56.45	Probable pectinesterase 48 OS=Arabidopsis thaliana GN=PME48 PE=2 SV=2	[more]
AL11B_OLEEU	1.0e-88	53.55	Pectinesterase 2 OS=Olea europaea PE=1 SV=1	[more]
AL11A_OLEEU	2.3e-88	52.90	Pectinesterase 1 OS=Olea europaea PE=1 SV=1	[more]

BLAST of Cucsa.086780 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KEY2_CUCSA	1.6e-186	100.00	Pectinesterase OS=Cucumis sativus GN=Csa_6G428540 PE=4 SV=1	[more]
A0A0A0KEH2_CUCSA	3.6e-141	79.23	Pectinesterase OS=Cucumis sativus GN=Csa_6G428030 PE=4 SV=1	[more]
A0A0A0KJZ4_CUCSA	1.5e-126	71.47	Pectinesterase OS=Cucumis sativus GN=Csa_6G428040 PE=4 SV=1	[more]
A0A0A0KI78_CUCSA	9.5e-126	70.83	Pectinesterase OS=Cucumis sativus GN=Csa_6G428290 PE=4 SV=1	[more]
M5XK08_PRUPE	2.1e-109	62.70	Pectinesterase OS=Prunus persica GN=PRUPE_ppa007533mg PE=4 SV=1	[more]

BLAST of Cucsa.086780 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G61680.1	5.0e-102	59.66	Pectin lyase-like superfamily protein	[more]
AT1G69940.1	1.9e-101	56.77	Pectin lyase-like superfamily protein	[more]
AT5G07410.1	1.2e-100	56.45	Pectin lyase-like superfamily protein	[more]
AT5G07420.1	2.2e-89	53.23	Pectin lyase-like superfamily protein	[more]
AT5G07430.1	2.4e-88	51.92	Pectin lyase-like superfamily protein	[more]

BLAST of Cucsa.086780 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778722333\|ref\|XP_004140274.2\|	2.4e-186	100.00	PREDICTED: putative pectinesterase 63 [Cucumis sativus]	[more]
gi\|659097432\|ref\|XP_008449623.1\|	2.2e-155	86.62	PREDICTED: putative pectinesterase 63 [Cucumis melo]	[more]
gi\|659097434\|ref\|XP_008449624.1\|	1.4e-141	79.49	PREDICTED: pectinesterase PPME1-like [Cucumis melo]	[more]
gi\|778716816\|ref\|XP_011657599.1\|	5.2e-141	79.23	PREDICTED: putative pectinesterase 63 [Cucumis sativus]	[more]
gi\|778716819\|ref\|XP_004140272.2\|	2.1e-126	71.47	PREDICTED: putative pectinesterase 63 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000070	Pectinesterase_cat
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005618	cell wall
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.086780.1	Cucsa.086780.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 18..304 score: 4.1
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 14..309 score: 2.69
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 14..310 score: 4.7
None	No IPR available	PANTHER	PTHR31321	FAMILY NOT NAMED	coord: 3..313 score: 9.2E
None	No IPR available	PANTHER	PTHR31321:SF23	PECTINESTERASE PPME1-RELATED	coord: 3..313 score: 9.2E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.086780	Melon (DHL92) v3.6.1	cgymedB097
Cucsa.086780	Silver-seed gourd	carcgyB1036
Cucsa.086780	Cucumber (Chinese Long) v3	cgycucB091
Cucsa.086780	Watermelon (97103) v2	cgywmbB093
Cucsa.086780	Wax gourd	cgywgoB110
Cucsa.086780	Cucurbita maxima (Rimu)	cgycmaB0166
Cucsa.086780	Cucurbita maxima (Rimu)	cgycmaB0167
Cucsa.086780	Cucurbita moschata (Rifu)	cgycmoB0161
Cucsa.086780	Cucurbita moschata (Rifu)	cgycmoB0162
Cucsa.086780	Wild cucumber (PI 183967)	cgycpiB092
Cucsa.086780	Cucumber (Chinese Long) v2	cgycuB089
Cucsa.086780	Melon (DHL92) v3.5.1	cgymeB098
Cucsa.086780	Watermelon (Charleston Gray)	cgywcgB096
Cucsa.086780	Watermelon (97103) v1	cgywmB099
Cucsa.086780	Cucurbita pepo (Zucchini)	cgycpeB0160
Cucsa.086780	Cucurbita pepo (Zucchini)	cgycpeB0161
Cucsa.086780	Bottle gourd (USVL1VR-Ls)	cgylsiB095