Cucsa.084850 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.084850 vs. Swiss-Prot
Match: PIEZO_ARATH (Piezo-type mechanosensitive ion channel homolog OS=Arabidopsis thaliana GN=At2g48040/At2g48050/At2g48060 PE=2 SV=1)

HSP 1 Score: 1859.7 bits (4816), Expect = 0.0e+00
Identity = 925/1372 (67.42%), Postives = 1099/1372 (80.10%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRV 60
            M ++SW++P+V+YFL +QLL   VA ADI+  R G     D+ W HF     HLGSHLRV
Sbjct: 96   MILKSWRNPTVMYFLALQLLTSLVALADIYSSRFGFARWRDTWWSHFSGIFEHLGSHLRV 155

Query: 61   ASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSI 120
            ASCLLLPA+Q+ VGI  PSWVSLPFFIGSC GLVDWSLTSN  GLFRWWR L +YAGF+I
Sbjct: 156  ASCLLLPAVQLAVGICNPSWVSLPFFIGSCAGLVDWSLTSNVSGLFRWWRVLYIYAGFNI 215

Query: 121  FLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEM 180
             L+Y+YQLP+ +  M++W+A FIGLF+IS  +E P+ICS + L+LFYIMLS V+ DLE+M
Sbjct: 216  VLLYLYQLPINFSDMIRWIASFIGLFRISLETEGPDICSGLFLVLFYIMLSYVRSDLEDM 275

Query: 181  DFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV 240
            DFIMS  E+NL E+LLP K+SFFIRE R+GV+HTNVLLR  VF+TF+INFFTYGFPVSL 
Sbjct: 276  DFIMSTSENNLAERLLPPKYSFFIRESRAGVRHTNVLLRGAVFKTFSINFFTYGFPVSLF 335

Query: 241  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFT 300
            ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFIL WA+STYIFNVAF+
Sbjct: 336  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILLWAVSTYIFNVAFS 395

Query: 301  FLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSS 360
            FLN K+GK                       FGLG+LVAL NLVNNSVFL LS E   SS
Sbjct: 396  FLNTKVGK-----------------------FGLGMLVALGNLVNNSVFLYLSEESSRSS 455

Query: 361  NDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLS 420
            N+ S      ETKVL+VATIAWGLRK SRAI+L LIFL+AMKPGF HAVYV+FFL+YLLS
Sbjct: 456  NERSYVEADEETKVLVVATIAWGLRKCSRAIMLALIFLIAMKPGFFHAVYVIFFLMYLLS 515

Query: 421  HDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFL 480
            H+++RK+R+SLILL  VHFALLY+L+I L+S+ L +EGS  RE+L QLGLL  +S W+FL
Sbjct: 516  HNINRKIRKSLILLCEVHFALLYILEIDLVSNSLKQEGSASREVLFQLGLLRSESSWDFL 575

Query: 481  EIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYC 540
            EIALLACFC IHNHGFE+LFSFSAIV HTPSPP+GFSIL+AGLNKSVLLSVY++ +S+Y 
Sbjct: 576  EIALLACFCAIHNHGFEVLFSFSAIVRHTPSPPIGFSILKAGLNKSVLLSVYSSPSSSYS 635

Query: 541  HDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVW 600
             DN ++ER IASFLS+IG+KFLSMYRSCGTYIAF+TIL++V+ VKPNY+SFGY+FLLL+W
Sbjct: 636  QDNTTYERHIASFLSAIGQKFLSMYRSCGTYIAFITILISVYLVKPNYVSFGYIFLLLLW 695

Query: 601  MIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASS 660
            + GRQL E TKRRLWFPLKAYA++VF+FIYCLSSF S ++WLS  IDL FYLG+NS+A  
Sbjct: 696  ITGRQLFEETKRRLWFPLKAYAVLVFMFIYCLSSFVSLQLWLSGFIDLYFYLGYNSKAPL 755

Query: 661  LQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALF 720
            L N W+SLAVLIVMQLYSYERRQS +    +  LL     GF +RFL WH  KILFAALF
Sbjct: 756  LDNVWESLAVLIVMQLYSYERRQSGHYIPGQSSLLHPGVFGFFERFLAWHGQKILFAALF 815

Query: 721  YASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFP 780
            YAS+SPIS FG +YLLGLVIC T PK S IPSK FL YTG +++ EYLFQ+WG QA MFP
Sbjct: 816  YASLSPISVFGFVYLLGLVICTTFPKSSSIPSKSFLIYTGFLVSAEYLFQLWGMQAQMFP 875

Query: 781  GQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKW 840
            GQK++ LS++LG R ++PGFWG+E GLRGKVL++AACTLQYNVFRWLER  G  + KGK+
Sbjct: 876  GQKYAELSFYLGLRVYEPGFWGIESGLRGKVLVVAACTLQYNVFRWLERTSGLTVIKGKY 935

Query: 841  DDPCPLFVTEED-DYDISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSAS 900
            ++PCPLFV+ ED    +S SN ++ SS+D   +  +Q          F    +Q      
Sbjct: 936  EEPCPLFVSAEDTTASVSSSNGENPSSTDHASISMKQGEATSNSWPFFSPRGNQGAGFLH 995

Query: 901  SKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENL 960
             K   SE  S+ K+SFG  WGSIKESH+W++ RI++L+KERFE QK + KIY+KFW+EN+
Sbjct: 996  PKTGGSESGSSRKFSFGHFWGSIKESHRWNRRRILALKKERFETQKNLLKIYLKFWIENM 1055

Query: 961  FNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILIL 1020
            FNL+GLEI MI+LLLASFALLN++S+ Y+ LLAAC+LL R +I+KLWP+ VFLFASIL +
Sbjct: 1056 FNLYGLEINMIALLLASFALLNAISMVYIALLAACVLLRRRVIQKLWPVVVFLFASILAI 1115

Query: 1021 EYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVF 1080
            EY+A W +   S+   PS+  VHCHDCW I+  YF+FC  CWLG+ VDD R L SYFVVF
Sbjct: 1116 EYVATWNSFLPSD-QAPSETSVHCHDCWSIAALYFKFCRECWLGVRVDDPRTLISYFVVF 1175

Query: 1081 MLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLL 1140
            ML+  KLRADH+S FS SSTY +M SQRKN+FVWRDLSFETKSMWT+LDYLRLYCY HLL
Sbjct: 1176 MLACFKLRADHISSFSESSTYHQMKSQRKNSFVWRDLSFETKSMWTVLDYLRLYCYVHLL 1235

Query: 1141 DLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLA 1200
            D+VL LILITGTLEYD+LHLGYLAFALVF R+RLEILKKKNK+F+FLR YNF LII SLA
Sbjct: 1236 DVVLILILITGTLEYDILHLGYLAFALVFARMRLEILKKKNKIFRFLRVYNFVLIIFSLA 1295

Query: 1201 YQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQ 1260
            YQSPFVG  + GKCET+ YI+E+IGFYKYDYGFRITARSALVEIIIFMLVS+QSYMFSSQ
Sbjct: 1296 YQSPFVGNFNDGKCETVDYIYEVIGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQ 1355

Query: 1261 EFEYVCRYLEAEQIGAIVREQEKKAAWKTEQLQHIRDSEERKRQRNLQVEKMKSEMLNLQ 1320
            EF+YV RYLEAEQIGAIVREQEKKAA KTEQLQ IR++EE+KRQRNLQVEKMKSEMLNL+
Sbjct: 1356 EFDYVSRYLEAEQIGAIVREQEKKAARKTEQLQQIREAEEKKRQRNLQVEKMKSEMLNLR 1415

Query: 1321 IQLHNMNSFVDGNNVSPSPGNESFRKRSTSGIHDD--AMTTDIEGTPGKKKK 1370
            +QLH MNS  D N    SP  E  R+R +  +  D  A + +I+G   +K++
Sbjct: 1416 VQLHRMNS--DSNFGVASPRTEGLRRRKSPYLIPDSGAASPEIDGVVHRKEE 1441

HSP 2 Score: 1412.1 bits (3654), Expect = 0.0e+00
Identity = 688/972 (70.78%), Postives = 815/972 (83.85%), Query Frame = 1

Query: 1366 KKKKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDD-NEQSKTED 1425
            +++K K   K NPL+SAVQ IGDGVSQVQ IGNQAV+NL +FLN+ P++ D NEQS  +D
Sbjct: 1494 RERKQKSEGKENPLISAVQLIGDGVSQVQFIGNQAVNNLVNFLNISPENSDTNEQSSVDD 1553

Query: 1426 RVYDQIESQETRYAHLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCY 1485
             VYD++ESQ+ ++     +  ERS+SLQSD+SSD  S Q+GRIFRHIW++M++NND+VCY
Sbjct: 1554 EVYDEMESQKRKH-----TPFERSTSLQSDRSSDGTSFQIGRIFRHIWSRMQSNNDIVCY 1613

Query: 1486 CCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQ 1545
            CCFI+ FLWNFSLLSM YLAALF+YALCV+TGP ++FWV+ML+YTE+YILLQYLYQIIIQ
Sbjct: 1614 CCFIIAFLWNFSLLSMVYLAALFLYALCVHTGPTHIFWVIMLMYTEIYILLQYLYQIIIQ 1673

Query: 1546 HCGLTINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSSAFN- 1605
            HCGL+I++ LL ELGFPT RI SSFVVSSLPLFL+Y+FTL+QSSIT KDG+W  S+ F  
Sbjct: 1674 HCGLSIDAPLLHELGFPTQRIKSSFVVSSLPLFLIYIFTLIQSSITVKDGDWVPSADFTS 1733

Query: 1606 -KNALPSKQSLGHYGLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSL 1665
             +NA  S++ L    L+ R  ++    R     V+RS+ +YW SLT+GAESPPYF+QV++
Sbjct: 1734 RRNARGSQKDLTRIRLSQRILDVFKKLRDSAKLVIRSIYRYWISLTRGAESPPYFVQVTM 1793

Query: 1666 DVQIWPEDGIQPERIESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIA 1725
            DV +WPEDGIQPER+E  +N +L+++H ERC++ NP LC +SSRVHVQSIERS E    A
Sbjct: 1794 DVHMWPEDGIQPERVECRMNQLLRLVHNERCEKGNPDLCPYSSRVHVQSIERSTETPNEA 1853

Query: 1726 LVVLEVVYASPSTNTC--AEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGG 1785
            LVVLEV YASP TN C  AEW  SLTPA+DVA EI  AQ +   E TGFPY ILSVIGGG
Sbjct: 1854 LVVLEVEYASP-TNGCSSAEWYKSLTPASDVAKEIRKAQHSGLGEGTGFPYPILSVIGGG 1913

Query: 1786 KREIDLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLI 1845
            KR+ DLYAY+FGAD+IVFFLVAIFYQS+IKN SEF+DVYQLEDQFP +FV +LM+IFFLI
Sbjct: 1914 KRDTDLYAYIFGADLIVFFLVAIFYQSVIKNKSEFIDVYQLEDQFPFDFVIILMVIFFLI 1973

Query: 1846 VLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSL 1905
            V+DR IYLCSFA GKV++YLF+L+LFTYAVTEYAW + P+ QHA  LALR IFLAKA+SL
Sbjct: 1974 VVDRVIYLCSFATGKVVYYLFSLILFTYAVTEYAWSIYPTQQHAAGLALRIIFLAKAMSL 2033

Query: 1906 ALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLT 1965
            ALQAIQIRYGLPHKSTLYRQFLTS+VSRINY GYRLYRALPFLYELRCVLDWSCT TSLT
Sbjct: 2034 ALQAIQIRYGLPHKSTLYRQFLTSEVSRINYYGYRLYRALPFLYELRCVLDWSCTATSLT 2093

Query: 1966 MYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLM 2025
            MYDWLKLED+NASLYLVKCD VLNR+ HK G+KQT MTKCCNGICLFFIL+CVIWAPMLM
Sbjct: 2094 MYDWLKLEDVNASLYLVKCDTVLNRATHKHGEKQTKMTKCCNGICLFFILLCVIWAPMLM 2153

Query: 2026 YSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPY 2085
            YSSGNPTN+ANPIKDAS Q+D+KT  G+LTLYQTTLCE+IS D ++  + L    +L  Y
Sbjct: 2154 YSSGNPTNIANPIKDASVQIDLKTVGGKLTLYQTTLCERISGDNIDLGLDLGSQSFLPTY 2213

Query: 2086 NQDDIQLICCQADASVLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYD 2145
            N++DIQLICCQADASVLWLVPD V +RF+ SLD   D+ I+FTW+L RDRPKGKE VKY+
Sbjct: 2214 NKNDIQLICCQADASVLWLVPDTVVTRFIQSLDWDTDMDITFTWVLNRDRPKGKETVKYE 2273

Query: 2146 RVIESRDLPNQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILN 2205
            R ++  DLP +SD+Q VLNGSM+GFR+ N+Y ++FRVTGSG+VR  E +   VSAD+++N
Sbjct: 2274 RSVDPLDLPKRSDIQMVLNGSMDGFRVHNLYPKFFRVTGSGDVRSFEDQTDEVSADILIN 2333

Query: 2206 RNNYE-WWSFHDIQPI-NVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFV 2265
              N++ WWSFH+++   N+S C    GPVA ++SEE PPQG LGDTLSKFSIWGLYITFV
Sbjct: 2334 HANFKWWWSFHNLKASENISACEGMDGPVAIIMSEETPPQGFLGDTLSKFSIWGLYITFV 2393

Query: 2266 LAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYWTLVKIYR 2325
            LAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICED+YAARAEGELGVEEVLYWTLVKIYR
Sbjct: 2394 LAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDLYAARAEGELGVEEVLYWTLVKIYR 2453

Query: 2326 SPHMLLEYTKVD 2331
            SPHMLLEYTK+D
Sbjct: 2454 SPHMLLEYTKLD 2459

BLAST of Cucsa.084850 vs. Swiss-Prot
Match: PIEZ2_HUMAN (Piezo-type mechanosensitive ion channel component 2 OS=Homo sapiens GN=PIEZO2 PE=1 SV=2)

HSP 1 Score: 209.1 bits (531), Expect = 5.0e-52
Identity = 154/576 (26.74%), Postives = 282/576 (48.96%), Query Frame = 1

Query: 1785 DLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQL-EDQFPKEFVFVLMIIFFLIVLD 1844
            D+Y  +F AD + F ++   + +  K+++       L EDQ P  F+ +++I F  +V+D
Sbjct: 2188 DVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVD 2247

Query: 1845 RCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPS---NQHAGELALRAIFLAKAVSL 1904
            R +YL    +GKVIF     V+  + +  + + + P     + +  L  +  +  K V  
Sbjct: 2248 RALYLRKTVLGKVIFQ----VILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYF 2307

Query: 1905 ALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLT 1964
             L A QIR G P +  +   FLT   + +N   ++ +R +PFL ELR V+DW  T T+L+
Sbjct: 2308 GLSAYQIRCGYPTR--VLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLS 2367

Query: 1965 MYDWLKLEDINASLYLVKC-DAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPML 2024
            +  W+ +EDI A ++++KC      R    +G K+  + K   G  +  +LIC++W P+L
Sbjct: 2368 LSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLL 2427

Query: 2025 MYSS-GNPTNVANPIKDASCQVDIKTTSGRLTLY----QTTLCEKISWDKLNTNMVLDPG 2084
              S   +   V N   D S  + +       T+     Q  + ++ S++K       D G
Sbjct: 2428 FMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTG 2487

Query: 2085 G--YLSPYNQDDIQLICCQADASVLWLVPDVVQSRFVHSL-DRKQDIIISFTWILTRDRP 2144
               +L  Y ++DI +   + +++ LW +    + + +H L D      + F+W + R+  
Sbjct: 2488 AMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLS 2547

Query: 2145 KG-KEVVKYDRVIESRDLPNQSDVQKVLNG-----SMNGFRIKNVYQRYFRVTGSGEVRP 2204
             G K  +  D++        + ++ K++ G     S     I+ +Y  Y +       +P
Sbjct: 2548 LGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKP 2607

Query: 2205 LEQ---EESFVSADLILNRNNY-----EWWSFHDIQPINVSECGRFTGPVA-----FVIS 2264
            ++Q   E +F+   +IL+R+N      EWW       +N++   R   P +      V +
Sbjct: 2608 IKQLLSENNFMDITIILSRDNTTKYNSEWWV------LNLTG-NRIYNPNSQALELVVFN 2667

Query: 2265 EEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICE 2324
            +++ P  +    L+ + I GLY + VL +G+F+R   S +   I +E LP+ DR++ +C 
Sbjct: 2668 DKVSPPSL--GFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCT 2727

Query: 2325 DIYAARAEGELGVEEVLYWTLVKIYRSPHMLLEYTK 2329
            DI+  R  GEL +EE LY  L+ +YRSP  ++++T+
Sbjct: 2728 DIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTR 2748

BLAST of Cucsa.084850 vs. Swiss-Prot
Match: PIEZ1_RAT (Piezo-type mechanosensitive ion channel component 1 OS=Rattus norvegicus GN=Piezo1 PE=1 SV=3)

HSP 1 Score: 203.0 bits (515), Expect = 3.6e-50
Identity = 162/590 (27.46%), Postives = 275/590 (46.61%), Query Frame = 1

Query: 1785 DLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQL--EDQFPKEFVFVLMIIFFLIVL 1844
            D+YA +F AD IV  +V IF       +S   D+     +DQ P+ F+F+L++ F  +V+
Sbjct: 1963 DVYALMFLAD-IVDIVVIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVI 2022

Query: 1845 DRCIYLCSFAIGKVIFYLFNLVLFTY-------AVTEYAWQMEPSNQHAGELALRAIFLA 1904
            DR +YL    +G + F +  +V           AVTE  ++     Q          +  
Sbjct: 2023 DRALYLRKTVLGNLAFQVVLVVAIHLWMFFILPAVTERMFRQNAVAQ--------LWYFV 2082

Query: 1905 KAVSLALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCT 1964
            K +  AL A QIR G P +  +   FLT   + +N   ++ +R +PFL ELR V+DW  T
Sbjct: 2083 KCIYFALSAYQIRCGYPTR--ILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWT 2142

Query: 1965 TTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHK-QGDKQTVMTKCCNGICLFFILICVI 2024
             T+L++ +W+ +EDI A+++++KC     +   + +G K+  + K   G  +   LI +I
Sbjct: 2143 DTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAII 2202

Query: 2025 WAPMLMYSS-GNPTNVANPIKDASCQVDIKTTSGRLTL--YQTTLCEKISWDKLNTNMVL 2084
            W P+L  S   +   V N   D +  + +       T+   Q ++      D    +   
Sbjct: 2203 WFPLLFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPEDYEELSQQF 2262

Query: 2085 DPGG----YLSPYNQDDIQLICCQADASVLWLVPDVVQSRFVHSL-DRKQDIIISFTWIL 2144
            DP      ++S Y+ +DI     +  +  LW +    +++  H L +   DI + FTW  
Sbjct: 2263 DPYPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKHELYNGTADITLRFTWNF 2322

Query: 2145 TRDRPKGKEVVKYDRVIESRDLPNQSDVQKVLNGSMNG-----FRIKNVYQRYFRVTGSG 2204
             RD  KG  V +Y     + +L   S  ++ L   + G       I +++ +Y R     
Sbjct: 2323 QRDLAKGGSV-EYTNEKHTLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGP 2382

Query: 2205 EVRPLEQ-----EESFVSADLILNRNNY-----------------EWWSFHDIQPINVSE 2264
            E  P++Q     EE ++   + L R                    EWW       I + +
Sbjct: 2383 EANPVKQLQPDEEEDYLGVRIQLRREQVGTGTSGEQAGTKASDFLEWWV------IELQD 2442

Query: 2265 CGRFTGPVAFVI-SEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPY 2324
            C      +  VI S+++ P  +    L+ + I GLY++ VL VG+F+R   SD+   I +
Sbjct: 2443 CQAECNLLPMVIFSDKVSPPSL--GFLAGYGIVGLYVSIVLVVGKFVRGFFSDISHSIMF 2502

Query: 2325 ENLPSCDRLIAICEDIYAARAEGELGVEEVLYWTLVKIYRSPHMLLEYTK 2329
            E LP  DR++ +C+DI+  R   EL +EE LY  L+ +YRSP  ++++T+
Sbjct: 2503 EELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTR 2532

BLAST of Cucsa.084850 vs. Swiss-Prot
Match: PIEZ1_HUMAN (Piezo-type mechanosensitive ion channel component 1 OS=Homo sapiens GN=PIEZO1 PE=1 SV=4)

HSP 1 Score: 202.6 bits (514), Expect = 4.7e-50
Identity = 153/572 (26.75%), Postives = 270/572 (47.20%), Query Frame = 1

Query: 1785 DLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQL-EDQFPKEFVFVLMIIFFLIVLD 1844
            D+YA +F AD++ F ++   + +  K+++       L +DQ P+ F+ +L+I F  +V+D
Sbjct: 1959 DVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVVD 2018

Query: 1845 RCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQ 1904
            R +YL    +GK+ F +  LVL  +    +             +  +  +  K +  AL 
Sbjct: 2019 RALYLRKTVLGKLAFQVA-LVLAIHLWMFFILPAVTERMFNQNVVAQLWYFVKCIYFALS 2078

Query: 1905 AIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYD 1964
            A QIR G P +  +   FLT   + +N   ++ +R +PFL ELR V+DW  T T+L++  
Sbjct: 2079 AYQIRCGYPTR--ILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSS 2138

Query: 1965 WLKLEDINASLYLVKCDAVLNRSQHK-QGDKQTVMTKCCNGICLFFILICVIWAPMLMYS 2024
            W+ +EDI A+++++KC     +   + +G K+  + K   G  +   LI +IW P+L  S
Sbjct: 2139 WMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMS 2198

Query: 2025 S-GNPTNVANPIKDASCQVDIKTTSGRLTL--YQTTLCEKISWDKLNTNMVLDPGG---- 2084
               +   V N   D +  + +       T+   Q ++    +      +   DP      
Sbjct: 2199 LVRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQ 2258

Query: 2085 YLSPYNQDDIQLICCQADASVLWLVPDVVQSRFVHSL-DRKQDIIISFTWILTRDRPKGK 2144
            ++S Y+ +DI     +  +  LW +    +++    L +   DI + FTW   RD  KG 
Sbjct: 2259 FISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGG 2318

Query: 2145 EVVKYDRVIESRDLPNQSDVQKVLNGSMNGFR-----IKNVYQRYFRVTGSGEVRPLEQ- 2204
              V+Y        L   S  ++ L   + G       I N++ +Y R     E  P++Q 
Sbjct: 2319 -TVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQL 2378

Query: 2205 ----EESFVSADLILNRNN-------YEWWSFHDIQPINVSECGRFTGPVAFVI-SEEIP 2264
                E  ++   + L R          EWW       I + EC      +  VI S+++ 
Sbjct: 2379 QPNEEADYLGVRIQLRREQGAGATGFLEWWV------IELQECRTDCNLLPMVIFSDKVS 2438

Query: 2265 PQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYA 2324
            P  +    L+ + I GLY++ VL +G+F+R   S++   I +E LP  DR++ +C+DI+ 
Sbjct: 2439 PPSL--GFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFL 2498

Query: 2325 ARAEGELGVEEVLYWTLVKIYRSPHMLLEYTK 2329
             R   EL +EE LY  L+ +YRSP  ++++T+
Sbjct: 2499 VRETRELELEEELYAKLIFLYRSPETMIKWTR 2518

BLAST of Cucsa.084850 vs. Swiss-Prot
Match: PIEZ2_MOUSE (Piezo-type mechanosensitive ion channel component 2 OS=Mus musculus GN=Piezo2 PE=1 SV=2)

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-49
Identity = 150/572 (26.22%), Postives = 275/572 (48.08%), Query Frame = 1

Query: 1785 DLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQL-EDQFPKEFVFVLMIIFFLIVLD 1844
            D+Y  +F AD + F ++   + +  K+++       L EDQ P  F+ +++I F  +V+D
Sbjct: 2258 DVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVD 2317

Query: 1845 RCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPS---NQHAGELALRAIFLAKAVSL 1904
            R +YL    +GKVIF     V+  + +  + + + P     + +  L  +  +  K V  
Sbjct: 2318 RALYLRKTVLGKVIFQ----VILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYF 2377

Query: 1905 ALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLT 1964
             L A QIR G P +  +   FLT   + +N   ++ +R +PFL ELR V+DW  T T+L+
Sbjct: 2378 GLSAYQIRCGYPTR--VLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLS 2437

Query: 1965 MYDWLKLEDINASLYLVKC-DAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPML 2024
            +  W+ +EDI A ++++KC      R    +G K+    K   G  +  +LIC++W P+L
Sbjct: 2438 LSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKAVKYGMGGMIIVLLICIVWFPLL 2497

Query: 2025 MYSS-GNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGG--- 2084
              S   +   V N   D S  + +       T+       K+  D    N  L   G   
Sbjct: 2498 FMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVM-DNSKYNEFLKSFGPNS 2557

Query: 2085 ----YLSPYNQDDIQLICCQADASVLWLVPDVVQSRFVHSL-DRKQDIIISFTWILTRDR 2144
                +L  Y ++D+ +   + +++ LW +    + + +  L D      + F+W + R+ 
Sbjct: 2558 GAMQFLENYEREDVTVAELEGNSNSLWTISPPSKQKMIQELTDPNSCFSVVFSWSIQRNM 2617

Query: 2145 PKG-KEVVKYDRVIESRDLPNQSDVQKVLNG-----SMNGFRIKNVYQRYFRVTGSGEVR 2204
              G K  +  D++     +  ++ + K++ G     S     I+ +Y  Y +       +
Sbjct: 2618 TLGAKAEIATDKLSFPLAVATRNSIAKMIAGNDTESSNTPVTIEKIYPYYVKAPSDSNSK 2677

Query: 2205 PLEQ---EESFVSADLILNRNNY-----EWWSFHDIQPINVSECGRFTGPVAFVISEEIP 2264
            P++Q   E +F++  +IL R+N      EWW  +       ++  +    V F  ++++ 
Sbjct: 2678 PIKQLLSENNFMNITIILFRDNVTKSNSEWWVLNLTGSRIFNQGSQALELVVF--NDKVS 2737

Query: 2265 PQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYA 2324
            P  +    L+ + I GLY + VL +G+F+R   S +   I +E LP+ DR++ +C DI+ 
Sbjct: 2738 PPSL--GFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFL 2797

Query: 2325 ARAEGELGVEEVLYWTLVKIYRSPHMLLEYTK 2329
             R  GEL +EE LY  L+ +YRSP  ++++T+
Sbjct: 2798 VRETGELELEEDLYAKLIFLYRSPETMIKWTR 2818

BLAST of Cucsa.084850 vs. TrEMBL
Match: K7K8F8_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G150200 PE=4 SV=1)

HSP 1 Score: 3370.1 bits (8737), Expect = 0.0e+00
Identity = 1682/2396 (70.20%), Postives = 1960/2396 (81.80%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRV 60
            M +Q+WKSP VIYFLV+QLLA+ VA  DI+  R  L    D  W HF S + HLGSHL+V
Sbjct: 96   MIVQTWKSPYVIYFLVIQLLALLVALVDIYGKRDFLKTWQDPSWGHFISIMEHLGSHLQV 155

Query: 61   ASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSI 120
            ASCLLLPAIQ++VGIS PSW SLPFFIGSCVGLVDWSLTSNFLGLFRWWR LQLYAGF+I
Sbjct: 156  ASCLLLPAIQLVVGISHPSWASLPFFIGSCVGLVDWSLTSNFLGLFRWWRLLQLYAGFTI 215

Query: 121  FLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEM 180
            FL+Y+YQLP+E PSM+ W+A+ IGL+KIS+NSEWP+ICS++SL+ +YIMLS +K DLEEM
Sbjct: 216  FLLYIYQLPMELPSMIHWMADLIGLYKISANSEWPKICSSISLMFYYIMLSFIKSDLEEM 275

Query: 181  DFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV 240
             FI+S  + +L EQLLPSKHSFFIRE RSGV+HTNVLLR  VFRTF+INFFTYGFPVSL 
Sbjct: 276  GFIISRTDCSLTEQLLPSKHSFFIRESRSGVRHTNVLLRGAVFRTFSINFFTYGFPVSLF 335

Query: 241  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFT 300
             LSFWSFHFASLCAFGLLAYVGYI+YAFPSLF+LHRLNGLLLVFILFWA+STYIFNVAFT
Sbjct: 336  VLSFWSFHFASLCAFGLLAYVGYIVYAFPSLFRLHRLNGLLLVFILFWAVSTYIFNVAFT 395

Query: 301  FLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSS 360
            FLN K+G+DM +WEMVGLWHYPIPGFFLLAQF LGILVAL NLVNNSVFLCLS E   SS
Sbjct: 396  FLNWKLGRDMKIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDEGGLSS 455

Query: 361  NDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLS 420
            ND SS    GETKVLIVATIAWGLRK SRAI+LTLIF +A+KPGFIHAVY++FFL+YLLS
Sbjct: 456  NDFSSVKVEGETKVLIVATIAWGLRKCSRAIMLTLIFFIAIKPGFIHAVYMIFFLMYLLS 515

Query: 421  HDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFL 480
            HDVSRKMRQ+LILL  +HF+LLY+LQI+LIS+ L+++GSL  E+++QLGL   DS W+FL
Sbjct: 516  HDVSRKMRQALILLCEIHFSLLYVLQINLISTALEKKGSLSMEVVMQLGLRKEDSAWDFL 575

Query: 481  EIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYC 540
            E+ALLACFC IHNHGFEMLFSFSAI++H P PP+GF IL+AGLNKSVLLSVY++++    
Sbjct: 576  EVALLACFCAIHNHGFEMLFSFSAIIQHAPGPPIGFGILKAGLNKSVLLSVYSSSSVRNS 635

Query: 541  HDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVW 600
             ++ S+ER+IAS+LS+IG+KFLS+YRSCGTYIAF+TILLTV+ V+PN ISFGY+FLLL+W
Sbjct: 636  DESLSYERRIASYLSAIGQKFLSIYRSCGTYIAFVTILLTVYMVRPNCISFGYIFLLLLW 695

Query: 601  MIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASS 660
            +IGRQLVERTKR+LW PLK YAI+VF+FIY LSSFSS  MWLS+ IDL FYLG++S+ASS
Sbjct: 696  IIGRQLVERTKRQLWLPLKVYAILVFIFIYSLSSFSSLEMWLSKLIDLYFYLGYDSKASS 755

Query: 661  LQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALF 720
              N W+SLAVLIVMQLYSYERR+++ +  D  + LE   LGFI+RF+IWHS KILF ALF
Sbjct: 756  FDNVWESLAVLIVMQLYSYERRKNKQNRQDHLDQLEPGALGFIRRFIIWHSQKILFIALF 815

Query: 721  YASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFP 780
            YAS++ ISAFG LYL+GL+ C+ LPK S IPSK FLAYTG ++T EY+FQMWG+QA MFP
Sbjct: 816  YASLNSISAFGFLYLVGLIFCSILPKTSSIPSKSFLAYTGFLVTAEYVFQMWGKQAKMFP 875

Query: 781  GQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKW 840
            GQK+S +S FLGF  FQPGFWGLE GLRGKVL+I ACTLQYNVFRWLERMP + L+KG+W
Sbjct: 876  GQKYSDISLFLGFHVFQPGFWGLESGLRGKVLVIVACTLQYNVFRWLERMPNTVLSKGQW 935

Query: 841  DDPCPLFVTEEDDY-DISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSAS 900
            ++PCPLFV  ED + D ++ NE+SKSS +S      +EG+         SG SQ   + S
Sbjct: 936  EEPCPLFVPTEDVFIDDAMCNEESKSSYNSNLPSAIKEGVSGKSLQIITSGLSQALDTPS 995

Query: 901  SKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENL 960
            SK  +S  SS+ KYSFGFIWGS KES KW+K RI++LRKERFE QK + K+Y+KFWMEN 
Sbjct: 996  SKTGDSSDSSSKKYSFGFIWGSSKESQKWNKKRIVALRKERFETQKTVLKVYLKFWMENT 1055

Query: 961  FNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILIL 1020
            FNLFGLEI MISLLL SFALLN++S+ Y+ LLAAC+LL+R II K+WPIFVFLFASILIL
Sbjct: 1056 FNLFGLEINMISLLLVSFALLNAISMMYIALLAACVLLNRHIICKVWPIFVFLFASILIL 1115

Query: 1021 EYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVF 1080
            EY+A WK+M   N    S+  + CHDCW+ S  +F +C  CWLGL VDD RML SYFVVF
Sbjct: 1116 EYLAIWKDMLPLNSHASSE--IRCHDCWKTSTLHFSYCQKCWLGLIVDDPRMLISYFVVF 1175

Query: 1081 MLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLL 1140
            ML+  KLRAD L  FS SSTYR++MSQR+NTFVWRDLSFETKSMWT +DYLRLYCYCHLL
Sbjct: 1176 MLACFKLRADRLPSFSGSSTYRQIMSQRRNTFVWRDLSFETKSMWTFVDYLRLYCYCHLL 1235

Query: 1141 DLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLA 1200
            DLVL LILITGTLEYD+LHLGYLAFAL+FFR+RLEILKKKNK+FKFLR YNFA+II+SLA
Sbjct: 1236 DLVLILILITGTLEYDILHLGYLAFALIFFRMRLEILKKKNKIFKFLRIYNFAVIIISLA 1295

Query: 1201 YQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQ 1260
            YQSPF+G  SAGKCET + I+EMIGFYKYDYGFRITARSA+VEIIIF+LVS+QSYMFSSQ
Sbjct: 1296 YQSPFIGGPSAGKCETANNIYEMIGFYKYDYGFRITARSAIVEIIIFVLVSLQSYMFSSQ 1355

Query: 1261 EFEYVCRYLEAEQIGAI-----------------VREQEKKAAWKTEQLQHIR------- 1320
            EF+YVCRYLEAEQIGAI                 +RE E+K   +  Q++ ++       
Sbjct: 1356 EFDYVCRYLEAEQIGAIVREQEKKAAWKTAQLQQIRESEEKKQQRNMQVEKMKSEMLNLQ 1415

Query: 1321 ------------------DSEERKRQRNLQ--------VEKMKSEMLNLQIQLHNMNSFV 1380
                              ++E  +R+R++         +   + ++L         +S  
Sbjct: 1416 TQLHSMNTSTNCIDGFSHNNEGLRRRRSVSLASNNDIGIPDKEDQVLGRLDHTIREDSVY 1475

Query: 1381 DGNNVSPS-------PGNESFRKRSTSGIHDDAMTTDIEGTP---GKKKKHKGSAKGNPL 1440
              N   PS       P  E + K S      +    DI+ T    GK++K KG AK NPL
Sbjct: 1476 PINLHEPSVCTNVETPSTEEYMKHSVDSDFCEITEVDIDTTSSDSGKREKFKGQAKENPL 1535

Query: 1441 MSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDD-NEQSKTEDRVYDQIESQETRYA 1500
             SAVQ IGDGVSQVQ IGNQAV+NL SFLN+ P+D D NE S  ED +YD++ESQ+T++ 
Sbjct: 1536 KSAVQLIGDGVSQVQFIGNQAVNNLVSFLNISPEDSDSNEHSNIEDSIYDEMESQKTQHI 1595

Query: 1501 HLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNFSLL 1560
            +++RS     SS+QSDKSSD A +QLGRIFR+IW QM +NNDVVCYCCF+LVFLWNFSLL
Sbjct: 1596 YMDRS-----SSVQSDKSSDAARLQLGRIFRYIWHQMCSNNDVVCYCCFVLVFLWNFSLL 1655

Query: 1561 SMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLLQEL 1620
            SM YL ALF+YALCVNTGP Y+FW++MLIYTELYILLQYLYQI+IQHCGL+I+  LL+EL
Sbjct: 1656 SMMYLGALFLYALCVNTGPSYIFWIIMLIYTELYILLQYLYQIVIQHCGLSIDPHLLREL 1715

Query: 1621 GFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSS--AFNKNALPSKQSLGHY 1680
            GFPTH+ITSSFVVSSLPLFLVYLFTL+Q SIT KDGEW  S+   F +  L +K     Y
Sbjct: 1716 GFPTHKITSSFVVSSLPLFLVYLFTLIQISITPKDGEWMSSTDFKFKRTDLHAKDDRTSY 1775

Query: 1681 GLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGIQPER 1740
               DRA++LL     M+  ++ S  +YWKSLTQGAESPPYF+QVS+DV  WPEDGIQPER
Sbjct: 1776 NWQDRAWDLLNQVINMVKLIIISFFRYWKSLTQGAESPPYFVQVSMDVNFWPEDGIQPER 1835

Query: 1741 IESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYASPSTN 1800
            IESGIN +L+I+H ++CK +NP LCSF+SRV+VQSIERS+E   +ALVV EVVYASP  +
Sbjct: 1836 IESGINQVLRIVHNDKCKAKNPNLCSFASRVNVQSIERSQEKPNVALVVFEVVYASPVID 1895

Query: 1801 -TCAEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVFGADM 1860
             +  EW  SLTPA+DVA EIL AQR  FVE  GFPYRILSVIGGGKREIDLYAY+F AD+
Sbjct: 1896 CSSTEWNKSLTPASDVAKEILKAQRAGFVEEMGFPYRILSVIGGGKREIDLYAYIFCADL 1955

Query: 1861 IVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSFAIGK 1920
            IVFFLVAIFYQS+IKN SEFL+VYQLEDQFPKE+VF+LM IFFLIVLDR IYLCSFA GK
Sbjct: 1956 IVFFLVAIFYQSVIKNKSEFLEVYQLEDQFPKEYVFMLMAIFFLIVLDRIIYLCSFATGK 2015

Query: 1921 VIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKS 1980
            V+FY+FNL+LFTY+VTEY WQ++PS Q   + ALRAIFLAKAVSL LQAIQI+YG+PHKS
Sbjct: 2016 VVFYIFNLILFTYSVTEYDWQLKPS-QRIAQFALRAIFLAKAVSLGLQAIQIQYGIPHKS 2075

Query: 1981 TLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2040
            TLYRQFLTS+VSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY
Sbjct: 2076 TLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2135

Query: 2041 LVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKD 2100
            LVKCD+VLNR  HKQG+KQT MTKCCNGICLFF+LICVIWAPMLMYSSGNPTN+ANPIKD
Sbjct: 2136 LVKCDSVLNRVTHKQGEKQTKMTKCCNGICLFFVLICVIWAPMLMYSSGNPTNIANPIKD 2195

Query: 2101 ASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQADAS 2160
            AS QVDIKT SGRL LYQTTLCE++ WD LN+N   DP GYL  YN++DIQLICCQADAS
Sbjct: 2196 ASFQVDIKTVSGRLNLYQTTLCERLRWDLLNSNANPDPYGYLDAYNKNDIQLICCQADAS 2255

Query: 2161 VLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQSDVQ 2220
             LWLVP VV++R +HSL+   D+ I FTWI +RDRPKGKEVVKY++ ++ + LP QSDVQ
Sbjct: 2256 TLWLVPLVVRTRLIHSLEWNTDMEIFFTWIFSRDRPKGKEVVKYEKAVDPQYLPTQSDVQ 2315

Query: 2221 KVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFHDIQPI 2280
            KVLNGSMN FRI NVY RYFRVTGSG+VRPLE E++ +SADLILNR  +EWW+F D  P 
Sbjct: 2316 KVLNGSMNSFRIYNVYPRYFRVTGSGDVRPLE-EDNALSADLILNREQFEWWAFRDFNPS 2375

Query: 2281 NVSE-CGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2331
            N+S  CG  TGP+A +ISEE PPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQC+DLRM
Sbjct: 2376 NLSGLCGGLTGPMAIIISEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCADLRM 2435

BLAST of Cucsa.084850 vs. TrEMBL
Match: K7LGZ8_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_10G023700 PE=4 SV=1)

HSP 1 Score: 3368.9 bits (8734), Expect = 0.0e+00
Identity = 1681/2396 (70.16%), Postives = 1958/2396 (81.72%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRV 60
            M +QSWKSP VIYFLV+QLLA+ VA  DI+     L    D  W HF S + HLGSHLRV
Sbjct: 96   MIVQSWKSPYVIYFLVIQLLALLVALVDIYGKSHFLKTWQDPSWGHFLSLIEHLGSHLRV 155

Query: 61   ASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSI 120
            ASCLLLPAIQ++VGIS PSW SLPFFIGSCVGLVDWSLTSNFLGLFRWWR LQLYAGF+I
Sbjct: 156  ASCLLLPAIQLVVGISHPSWASLPFFIGSCVGLVDWSLTSNFLGLFRWWRLLQLYAGFNI 215

Query: 121  FLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEM 180
            FL+Y+YQLP+E PSM++W+A+ IGL+KIS+NSEWP+ICS++SL+ +YIMLS +K DLEEM
Sbjct: 216  FLLYIYQLPMELPSMIRWMADLIGLYKISANSEWPKICSSISLMFYYIMLSFIKSDLEEM 275

Query: 181  DFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV 240
             FI+S  + +L EQLLPSKHSFFIRE RSGV+HTNVLLR  VFRTF INFFTYGFPVSL 
Sbjct: 276  SFIISRTDCSLTEQLLPSKHSFFIRESRSGVRHTNVLLRGAVFRTFGINFFTYGFPVSLF 335

Query: 241  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFT 300
            ALSFWSFHFASLCAFGLLAYVGYI YAFPSLF++HRLNGLLLVFIL WA+STYIFNVAFT
Sbjct: 336  ALSFWSFHFASLCAFGLLAYVGYIAYAFPSLFRMHRLNGLLLVFILLWAVSTYIFNVAFT 395

Query: 301  FLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSS 360
            FLN K+G+DM +WEMVGLWHYPIPGFFLLAQF LGILVAL NLVNNSVFLCLS E   SS
Sbjct: 396  FLNWKLGRDMKIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDEGGQSS 455

Query: 361  NDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLS 420
            ND+SS    GETKVLIVAT+AWGLRK SRAI+LTLIF +A+KPGFIHAVY++FFL+YLLS
Sbjct: 456  NDHSSVKVEGETKVLIVATVAWGLRKCSRAIMLTLIFFIAIKPGFIHAVYMIFFLVYLLS 515

Query: 421  HDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFL 480
            H+VSRKMRQ+LILL  +HF+LLY+LQI+LIS+ L+++GSL  EI++QLGL   DS W+FL
Sbjct: 516  HNVSRKMRQALILLCEIHFSLLYVLQINLISTALEKKGSLSMEIVMQLGLRNEDSAWDFL 575

Query: 481  EIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYC 540
            E+ALLACFC IHNHGFEMLFSFSAI++H PSPP+GF IL+AGLNKSVLLSVY++++    
Sbjct: 576  EVALLACFCAIHNHGFEMLFSFSAIIQHAPSPPIGFGILKAGLNKSVLLSVYSSSSVRNS 635

Query: 541  HDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVW 600
             ++ S+ER+IAS+LS+IG+KFLS+YRSCG+++AF+TILLTV+ V+PNYISFGY+FLLL+W
Sbjct: 636  DESLSYERRIASYLSAIGQKFLSIYRSCGSHVAFVTILLTVYMVRPNYISFGYIFLLLLW 695

Query: 601  MIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASS 660
            +IGRQLVERTKR+LW PLK YAI+VF+FIY LSSFSS  MWLS+ IDL  YLG++S+ASS
Sbjct: 696  IIGRQLVERTKRQLWLPLKVYAILVFIFIYSLSSFSSLEMWLSKLIDLYLYLGYDSKASS 755

Query: 661  LQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALF 720
              N W+SLAVLIVMQLYSYERR+++ +  D  + LE   LGFI+RF+IWHS KILF ALF
Sbjct: 756  FDNVWESLAVLIVMQLYSYERRKNKQNRQDHLDQLEPGALGFIRRFIIWHSQKILFIALF 815

Query: 721  YASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFP 780
            YAS++ ISAFG LYL+GL+ C+ LPK S IPSK FLAYTG ++T EYLFQMWG+QA MFP
Sbjct: 816  YASLNSISAFGFLYLIGLIFCSILPKTSSIPSKSFLAYTGFLVTAEYLFQMWGKQAKMFP 875

Query: 781  GQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKW 840
            GQK+S +S FLGF  FQPGFWGLE GLRGKVL+I ACTLQYNVF WLERMP + L+KG+W
Sbjct: 876  GQKYSDISLFLGFHVFQPGFWGLESGLRGKVLVIVACTLQYNVFHWLERMPNTVLSKGQW 935

Query: 841  DDPCPLFVTEEDDY-DISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSAS 900
            ++PCPLFV  ED + D +  NE+SKSS +S      +EG+         SG SQ P + S
Sbjct: 936  EEPCPLFVPTEDAFIDDAKCNEESKSSYNSQLPSAIKEGVSGNSLQIITSGLSQAPDTPS 995

Query: 901  SKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENL 960
            SK + S  SS+ KYSFGFIWGS KESHKW+K RI++LRKERFE QK + K+Y+KFWMEN 
Sbjct: 996  SKTEGSSDSSSKKYSFGFIWGSSKESHKWNKKRIVALRKERFETQKTVLKVYLKFWMENT 1055

Query: 961  FNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILIL 1020
            FNLFGLEI MISLLL SFALLN++S+ Y+ LLAAC+LL+R IIRK+WPIFVFLFASILIL
Sbjct: 1056 FNLFGLEINMISLLLVSFALLNALSMLYIALLAACVLLNRHIIRKVWPIFVFLFASILIL 1115

Query: 1021 EYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVF 1080
            EY+A WK+M   N    S+  + C DCW+ S  +F +C  CWLGL VDD RML SYFVVF
Sbjct: 1116 EYLAIWKDMLPLNSHASSE--IRCRDCWKTSTLHFSYCKKCWLGLIVDDPRMLISYFVVF 1175

Query: 1081 MLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLL 1140
            ML+  KLRAD L  FS SSTYR++MSQR+NTFVWRDLSFETKSMWT +DYLRLYCYCHLL
Sbjct: 1176 MLACFKLRADRLPSFSGSSTYRQIMSQRRNTFVWRDLSFETKSMWTFVDYLRLYCYCHLL 1235

Query: 1141 DLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLA 1200
            DLVL LILITGTLEYD+LHLGYLAFAL+FFR+RLEILKKKNK+FKFLR YNFA+II SLA
Sbjct: 1236 DLVLILILITGTLEYDILHLGYLAFALIFFRMRLEILKKKNKIFKFLRIYNFAVIITSLA 1295

Query: 1201 YQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQ 1260
            YQSPF+G +SAGKCET++ I+EMIGFYKYDYGFRITARSA+VEIIIF+LVS+QSYMFSSQ
Sbjct: 1296 YQSPFIGGLSAGKCETVNDIYEMIGFYKYDYGFRITARSAIVEIIIFVLVSLQSYMFSSQ 1355

Query: 1261 EFEYVCRYLEAEQIGAI-----------------VREQEKKAAWKTEQLQHIRD------ 1320
            EF+YVCRYLEAEQIGAI                 +RE E+K   +  Q++ ++       
Sbjct: 1356 EFDYVCRYLEAEQIGAIVREQEKKAAWKTAQLQQIRESEEKKQQRNMQVEKMKSEMLNLQ 1415

Query: 1321 ------------------SEERKRQR---------NLQVEKMKSEMLNLQIQLHNMNSFV 1380
                              S E  R+R         ++ +   + ++L         +S  
Sbjct: 1416 IQLLGMNTSTNCIDGFSHSNEGLRRRRSVSLASNNDIGIPDKEDQVLGRLDHTIREDSVY 1475

Query: 1381 DGNNVSPS-------PGNESFRKRSTSGIHDDAMTTDIEGTP---GKKKKHKGSAKGNPL 1440
              N   PS       P  E + K S      +    DI+ +    GKK+K KG AK NPL
Sbjct: 1476 PINLHEPSACTNVESPLTEDYMKHSVDSPFCEITEIDIDTSSSDSGKKEKFKGQAKENPL 1535

Query: 1441 MSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDD-NEQSKTEDRVYDQIESQETRYA 1500
             SAVQ IGDGVSQVQ IGNQAV+NL SFLN+  +D D NE +  EDR+YD++ESQ+TR+ 
Sbjct: 1536 KSAVQLIGDGVSQVQFIGNQAVNNLVSFLNISQEDSDSNEHTNIEDRIYDEMESQKTRHI 1595

Query: 1501 HLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNFSLL 1560
            +++RS     SS+QSDKSSD AS+QLGRIFR+IW QMR+NNDVVCY CF+LVFLWNFSLL
Sbjct: 1596 YMDRS-----SSVQSDKSSDAASLQLGRIFRYIWHQMRSNNDVVCYFCFVLVFLWNFSLL 1655

Query: 1561 SMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLLQEL 1620
            SM YL ALF+YALCVNTGP Y+FW++MLIYTELYILLQYLYQI+IQHCGL+IN  LL+EL
Sbjct: 1656 SMVYLGALFLYALCVNTGPSYIFWIIMLIYTELYILLQYLYQIVIQHCGLSINPHLLREL 1715

Query: 1621 GFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSS--AFNKNALPSKQSLGHY 1680
            GFPTH+ITSSFVVSSLPLFLVYLFTL+QSSIT KDGEW  S+   F +N L +K     Y
Sbjct: 1716 GFPTHKITSSFVVSSLPLFLVYLFTLIQSSITPKDGEWMSSTDFKFKRNDLHAKDDHTSY 1775

Query: 1681 GLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGIQPER 1740
                RA +LL     M+  ++ S  +YWKSLTQGAESPPYF+QVS+DV  WPEDGIQPER
Sbjct: 1776 NWQGRARDLLNQMIIMVKLIIISFFRYWKSLTQGAESPPYFVQVSMDVNFWPEDGIQPER 1835

Query: 1741 IESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYASPSTN 1800
            IESGIN +L+I+H ++CK +NP LCSF+SRV+VQSIERS+E   +ALVV EVVYASP  +
Sbjct: 1836 IESGINQVLRIVHNDKCKAKNPNLCSFASRVNVQSIERSQEKPNVALVVFEVVYASPVID 1895

Query: 1801 -TCAEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVFGADM 1860
             +  EW  SLTPA+DVA EIL AQR  FVE  GFPYRILSVIGGGKREIDLYAY+F AD+
Sbjct: 1896 CSSTEWNKSLTPASDVAKEILKAQRAGFVEEMGFPYRILSVIGGGKREIDLYAYIFCADL 1955

Query: 1861 IVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSFAIGK 1920
            IVFFLVAIFYQS+IKN SEFL+VYQLEDQFPKE+VF+LM IFFLIVLDR +YLCSFA  K
Sbjct: 1956 IVFFLVAIFYQSVIKNKSEFLEVYQLEDQFPKEYVFMLMAIFFLIVLDRILYLCSFATWK 2015

Query: 1921 VIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKS 1980
            V+FY+FNLVLFTY+VTEY WQ+EPS QH  + ALRAIFLAKAVSL LQAIQI+YG+PHKS
Sbjct: 2016 VVFYIFNLVLFTYSVTEYDWQLEPSQQHTAQFALRAIFLAKAVSLGLQAIQIQYGIPHKS 2075

Query: 1981 TLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2040
            TLYRQFLTS+VSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY
Sbjct: 2076 TLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2135

Query: 2041 LVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKD 2100
            LVKCD+VLNR  HKQG+KQT MTKCCNGICLFF+LICVIWAPMLMYSSGNPTN+ANPIKD
Sbjct: 2136 LVKCDSVLNRGTHKQGEKQTKMTKCCNGICLFFVLICVIWAPMLMYSSGNPTNIANPIKD 2195

Query: 2101 ASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQADAS 2160
            AS QVDIKT SGRL LYQTTLCE++ WD LN+N+  DP GYL  YN++DIQLICCQADAS
Sbjct: 2196 ASFQVDIKTASGRLNLYQTTLCERLQWDLLNSNINPDPYGYLGAYNKNDIQLICCQADAS 2255

Query: 2161 VLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQSDVQ 2220
             LWLVP VV++R + SL+   D+ I  TWIL+RDRPKGKE+VKY++ ++ + LP +SDVQ
Sbjct: 2256 TLWLVPLVVRTRLIQSLEWNIDMEIFSTWILSRDRPKGKEIVKYEKAVDPQYLPTRSDVQ 2315

Query: 2221 KVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFHDIQPI 2280
            KVLNGSMN F I NVY RYFRVTGSG+VRPLE E++ VSADLI+NR   EWW+F D  P 
Sbjct: 2316 KVLNGSMNSFSIYNVYPRYFRVTGSGDVRPLE-EDNAVSADLIINREQLEWWAFRDTNPS 2375

Query: 2281 NVSE-CGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2331
            N+S  CG  TGP+A ++SEE PPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQC+DLRM
Sbjct: 2376 NLSRLCGGLTGPMAIIVSEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCADLRM 2435

BLAST of Cucsa.084850 vs. TrEMBL
Match: V7BFE5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G168500g PE=4 SV=1)

HSP 1 Score: 3348.5 bits (8681), Expect = 0.0e+00
Identity = 1673/2396 (69.82%), Postives = 1956/2396 (81.64%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRV 60
            M +QSWKSP VIYFLV+QLLA+ VA  DI+  R  L    D CW HF S + H+GSHLRV
Sbjct: 96   MIVQSWKSPYVIYFLVIQLLALLVALVDIYGKRHFLKTWQDWCWGHFLSIIEHIGSHLRV 155

Query: 61   ASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSI 120
            ASCLLLPAIQ++VGIS PSW SLPFFIGSCVGLVDWSLTSNFLGLFRWWR LQLYAGF+I
Sbjct: 156  ASCLLLPAIQLVVGISHPSWASLPFFIGSCVGLVDWSLTSNFLGLFRWWRLLQLYAGFNI 215

Query: 121  FLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEM 180
            FL+Y+YQLP+E PSM+ W+A+ IGL+KIS+NSEWP++CS++SL+ +Y MLS +K DLEEM
Sbjct: 216  FLLYIYQLPMELPSMIHWMADLIGLYKISANSEWPQVCSSISLLFYYTMLSFIKSDLEEM 275

Query: 181  DFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV 240
             FI+S  +  L EQLLPSKHSFFIRE RSGV+HTNVLLR  VFRTF+INFFTYGFPVSL 
Sbjct: 276  GFIISRTDCTLTEQLLPSKHSFFIRESRSGVRHTNVLLRGAVFRTFSINFFTYGFPVSLF 335

Query: 241  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFT 300
            ALSFWSFHFASLCAFGLLAYVGYI+YAFPSLF+LHRLNGLLLVFIL WA+STYIFNVAFT
Sbjct: 336  ALSFWSFHFASLCAFGLLAYVGYIVYAFPSLFRLHRLNGLLLVFILLWAVSTYIFNVAFT 395

Query: 301  FLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSS 360
            FLN K+G+DM +WEMVGLWHYPIPGFFLLAQF LGILVAL NLVNNSVFL LSGE   SS
Sbjct: 396  FLNWKLGRDMKIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLYLSGEGGQSS 455

Query: 361  NDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLS 420
            ND+SS    GETKVLIVATIAWGLRK SRAI+LTLIFL+AMKPGFIHAVY++FFL+YLLS
Sbjct: 456  NDHSSVKVEGETKVLIVATIAWGLRKCSRAIMLTLIFLIAMKPGFIHAVYMIFFLMYLLS 515

Query: 421  HDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFL 480
            H+VS K+RQ+LILL  +HF+LLY+L+I+LIS+ L+++GSL  E+++QLGL   DS W+FL
Sbjct: 516  HNVSGKIRQALILLCEIHFSLLYVLRINLISAALEKKGSLSMEVVMQLGLREEDSAWDFL 575

Query: 481  EIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYC 540
            E+ALLACFC IHN+GFEMLFSFSAI++H PSPP+GF IL+AGLNKSVLLSVY++++    
Sbjct: 576  EVALLACFCAIHNYGFEMLFSFSAIIQHAPSPPIGFGILKAGLNKSVLLSVYSSSSVRNN 635

Query: 541  HDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVW 600
             +N SHER++AS+LS+IG+KFLS+YRSCG+YIAF+TILLTV+ V+PNYISFGY+ LLL+W
Sbjct: 636  DENFSHERRVASYLSTIGQKFLSLYRSCGSYIAFVTILLTVYMVRPNYISFGYVLLLLLW 695

Query: 601  MIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASS 660
            +IGRQLVERT+R+LW PLK YAI+VF+FIY LSSFSS +MWLS+ IDL F LG++S+ASS
Sbjct: 696  IIGRQLVERTERQLWLPLKVYAILVFIFIYSLSSFSSLKMWLSKLIDLYFCLGYDSKASS 755

Query: 661  LQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALF 720
              N W+S+AVLIVMQLYSYERR++     D  + L    LGFI+RF+IWHS KILF ALF
Sbjct: 756  FDNVWESMAVLIVMQLYSYERRKNTQIRQDHLDQLGPGALGFIRRFIIWHSQKILFIALF 815

Query: 721  YASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFP 780
            YAS+S ISAFG LYL+GLV C+ LPK+S IPSK FLAYTG ++T EYLFQ+ G+QA MFP
Sbjct: 816  YASLSSISAFGFLYLIGLVFCSILPKVSTIPSKSFLAYTGFLVTAEYLFQLLGKQAKMFP 875

Query: 781  GQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKW 840
            GQK+S LS FLGF  FQPGFWGLE GLRGKVL+I ACTLQYNVFRWLERMP   L+KG+W
Sbjct: 876  GQKYSDLSLFLGFHVFQPGFWGLESGLRGKVLVIVACTLQYNVFRWLERMPNEVLSKGQW 935

Query: 841  DDPCPLFVTEEDDY-DISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSAS 900
            ++PCPLFV  ED + + ++ NE+SK S +S       E +         SG SQ P + S
Sbjct: 936  EEPCPLFVPTEDAFINDAMCNEESKGSYNSHPPSAVNERVPSRSLQIITSGLSQAPDTPS 995

Query: 901  SKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENL 960
            SK   S+ S++ KYSFGFIWGS KESHKW+K RII+LRKERFE QK + KIY+KFWMEN+
Sbjct: 996  SKTGGSD-SNSKKYSFGFIWGSSKESHKWNKKRIIALRKERFETQKTVLKIYLKFWMENM 1055

Query: 961  FNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILIL 1020
            FNLFGLEI MISLLL SFALLN++S+ Y+ LLAACILL+R IIRK+WPIFVFLFASILIL
Sbjct: 1056 FNLFGLEINMISLLLVSFALLNALSMLYIALLAACILLNRQIIRKVWPIFVFLFASILIL 1115

Query: 1021 EYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVF 1080
            EY+  WK+M  SN    ++  +HCHDCW+IS  YF +C  CW GL VDD RML SYFVVF
Sbjct: 1116 EYVVIWKDMKPSNSHASNE--IHCHDCWKISTLYFHYCEKCWFGLIVDDPRMLISYFVVF 1175

Query: 1081 MLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLL 1140
            ML+  KLRAD L  FS SSTYR++MSQR+NTFVWRDLSFETKSMWT LDYLRLYCYCHLL
Sbjct: 1176 MLACFKLRADRLPSFSGSSTYRQIMSQRRNTFVWRDLSFETKSMWTFLDYLRLYCYCHLL 1235

Query: 1141 DLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLA 1200
            DLVL LILITGTLEYD+LHLGYLAFALVFFR+R EILKKKN++FKFLR YNF +II+SLA
Sbjct: 1236 DLVLILILITGTLEYDILHLGYLAFALVFFRMRFEILKKKNRIFKFLRIYNFTVIIISLA 1295

Query: 1201 YQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQ 1260
            YQSPF+G  SAGKCET++ I+EMIGFYKYDYGFRITARSA+VEIIIF+LVS+QSYMFSSQ
Sbjct: 1296 YQSPFIGGPSAGKCETVNKIYEMIGFYKYDYGFRITARSAIVEIIIFVLVSLQSYMFSSQ 1355

Query: 1261 EFEYVCRYLEAEQ------------IGAIVREQEKKAAWKTEQLQHIRDSEERKRQRNLQ 1320
            EF+YVCRYLEAEQ                 + Q+ + + + ++ ++ +  + +    NLQ
Sbjct: 1356 EFDYVCRYLEAEQIGAIVREQEKKAAWKTAQLQQNRESEENKRQRNFQVEKMKSEMLNLQ 1415

Query: 1321 VE--------------KMKSEMLNLQIQ------------------LHNMNSFVDGNNVS 1380
            ++                 SE L  +                    L  ++S +  ++V 
Sbjct: 1416 IQLHSMNGSTNCIDGFSHNSEGLRRRRSVSLTSNNDIGISDKEDQVLGRLDSAIREDSVH 1475

Query: 1381 P-------------SPGNESFRKRSTSGIHDDAMTTDIE---GTPGKKKKHKGSAKGNPL 1440
            P             +P  E + K S      +    DI+      GKK+K KG  K NPL
Sbjct: 1476 PCELQEPSACTNVETPLTEEYMKHSLDSPICEITEIDIDTASSDSGKKEKVKGQPKENPL 1535

Query: 1441 MSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDDN-EQSKTEDRVYDQIESQETRYA 1500
             SAVQ IGDGVSQVQ IGNQAV+NL SFLN+  +D D+ E++  EDR+YD++ESQ+ R+ 
Sbjct: 1536 KSAVQLIGDGVSQVQFIGNQAVNNLVSFLNISHEDSDSHERTNIEDRIYDEMESQKNRHI 1595

Query: 1501 HLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNFSLL 1560
            +++RS     SS+QSDKSSD AS+QLGRIFR+IW QMR+NNDVVCYC F+LVFLWNFSLL
Sbjct: 1596 YMDRS-----SSMQSDKSSDAASLQLGRIFRYIWNQMRSNNDVVCYCSFVLVFLWNFSLL 1655

Query: 1561 SMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLLQEL 1620
            SM Y+ ALF+YALCV+TGP Y+FW++MLIYTELYILLQYLYQIIIQHCGL+I+  LL+EL
Sbjct: 1656 SMVYIGALFLYALCVHTGPSYIFWIIMLIYTELYILLQYLYQIIIQHCGLSIDPSLLREL 1715

Query: 1621 GFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSSAFN--KNALPSKQSLGHY 1680
            GFPTH+ITSSFVVSSLPLFLVYLFTL+QSSIT KD EW  S+ F   +N L +K     Y
Sbjct: 1716 GFPTHKITSSFVVSSLPLFLVYLFTLIQSSITPKDDEWISSTHFKYKRNDLHAKDDPTSY 1775

Query: 1681 GLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGIQPER 1740
               DRA++LL     M+  V+RS  +YWKSLTQGAESPPYF+QVSLDV  WPEDGIQP+R
Sbjct: 1776 NWQDRAWDLLNQMINMVKLVIRSFFRYWKSLTQGAESPPYFVQVSLDVNFWPEDGIQPQR 1835

Query: 1741 IESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYASPSTN 1800
            IESGIN +L+I+H E CKEQNP LCSF+SRV+VQSIERS E   +ALVV EVVYASP T+
Sbjct: 1836 IESGINQVLRIVHTENCKEQNPNLCSFASRVNVQSIERSLEKPNVALVVFEVVYASPVTD 1895

Query: 1801 TCA-EWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVFGADM 1860
            + + EW  SLTPAADVA EIL AQR   VE  GFPY ILSVIGGGKR+IDLYAY+F AD+
Sbjct: 1896 SSSTEWNKSLTPAADVAKEILKAQRAGLVEEVGFPYHILSVIGGGKRQIDLYAYIFCADL 1955

Query: 1861 IVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSFAIGK 1920
            IVFFLVAIFYQS++KN SEFLDVYQLEDQFPKE+VF+LM IFFLIVLDR IYLCSFA GK
Sbjct: 1956 IVFFLVAIFYQSVLKNKSEFLDVYQLEDQFPKEYVFILMAIFFLIVLDRIIYLCSFATGK 2015

Query: 1921 VIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKS 1980
            V+FY+FNLVLFTY+VTEYAWQ+EPS Q   + ALRAIFLAKAVSL LQA+QI+YG+PH+S
Sbjct: 2016 VVFYIFNLVLFTYSVTEYAWQLEPSQQRTAQFALRAIFLAKAVSLGLQAVQIQYGIPHQS 2075

Query: 1981 TLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2040
            TLYRQFLTS+VSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY
Sbjct: 2076 TLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2135

Query: 2041 LVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKD 2100
            LVKCD+VLNR+ HKQG+KQT MTKCCNGICLFF+LICVIWAPMLMYSSGNPTN+ANPIK+
Sbjct: 2136 LVKCDSVLNRATHKQGEKQTKMTKCCNGICLFFVLICVIWAPMLMYSSGNPTNIANPIKE 2195

Query: 2101 ASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQADAS 2160
            A+ QVDIKT SGRL LYQTTLCE++ WD L++N+  DP GYL  YN++DIQLICCQADAS
Sbjct: 2196 ATFQVDIKTVSGRLNLYQTTLCERLRWDLLDSNVNSDPFGYLDAYNKNDIQLICCQADAS 2255

Query: 2161 VLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQSDVQ 2220
             LWLVP VVQ+R + SL+   D+ I FTWIL+RDRPKGKEVVKY++ ++ + LP QSDVQ
Sbjct: 2256 TLWLVPLVVQTRLIQSLEWNTDMEIFFTWILSRDRPKGKEVVKYEKAVDPQYLPTQSDVQ 2315

Query: 2221 KVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFHDIQPI 2280
            +V NGS+N FRI NVY RYFR+TGSG+VRPLE E + VSADLI+NR  +EWW+F DI   
Sbjct: 2316 RVFNGSINSFRIDNVYPRYFRLTGSGDVRPLE-EANAVSADLIINREQFEWWTFRDINRS 2375

Query: 2281 NVSE-CGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2331
            N+S  CG  TGP+A +ISEE PPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM
Sbjct: 2376 NLSGLCGGLTGPMAIIISEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2435

BLAST of Cucsa.084850 vs. TrEMBL
Match: A0A059AZT0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H01545 PE=4 SV=1)

HSP 1 Score: 3263.4 bits (8460), Expect = 0.0e+00
Identity = 1631/2396 (68.07%), Postives = 1924/2396 (80.30%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRV 60
            +T+ SWK PS IYFL + LLA+ VA   I W R G +   DSCW  F S + HLGS LR+
Sbjct: 96   LTVHSWKKPSAIYFLALHLLAVTVAFLQINWNRFGPIISPDSCWGSFVSVIQHLGSRLRI 155

Query: 61   ASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSI 120
            A CL+LPAIQ++VGIS PSW+SLPFF+GSCVGLVDWSL+SNFLGLFRWWR LQLYAGF+I
Sbjct: 156  AFCLVLPAIQLVVGISYPSWISLPFFVGSCVGLVDWSLSSNFLGLFRWWRALQLYAGFNI 215

Query: 121  FLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEM 180
             L+YVYQLPV++P +  W+A+FIGL+KIS+ +EWP+ICS +SL+LFY+MLS VKC+LEEM
Sbjct: 216  VLLYVYQLPVDFPEISNWIADFIGLYKISTTTEWPKICSGLSLVLFYMMLSHVKCNLEEM 275

Query: 181  DFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV 240
            D I+S  ES+L E LLPS +SFFIRE RSGV+HTNVLLR  VFRTF+INFF YGFPVSL 
Sbjct: 276  DLILSTNESSLTEHLLPS-NSFFIRESRSGVRHTNVLLRGSVFRTFSINFFAYGFPVSLF 335

Query: 241  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFT 300
            ALSFWSFHFAS+CAFGLLAYVGYI+YAFPSLF+LHRLNGLLLVFIL WA++TY+FNVAF 
Sbjct: 336  ALSFWSFHFASICAFGLLAYVGYIVYAFPSLFRLHRLNGLLLVFILLWAVATYVFNVAFA 395

Query: 301  FLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSS 360
            F   K+GKD ++WEMVGLWHYP+PGFFLLAQF LG+LVAL NLVNNSVFL LS E     
Sbjct: 396  FFKWKLGKDTEIWEMVGLWHYPVPGFFLLAQFCLGVLVALGNLVNNSVFLSLSDEYGRHP 455

Query: 361  NDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLS 420
            ND+S  GE  +TKVLIVAT+AWGLRK SRAI+L LIFL+AMKPGFIHAVY++FFL+YLL 
Sbjct: 456  NDDSY-GEVEDTKVLIVATVAWGLRKCSRAIMLALIFLIAMKPGFIHAVYMIFFLVYLLG 515

Query: 421  HDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFL 480
            H++ R++RQSLILL   HFALLY+L+ISLIS  L+   SL  +IL QLGLL RDS W+FL
Sbjct: 516  HEIGRQIRQSLILLCEAHFALLYILRISLISRTLEDTSSLSMQILSQLGLLDRDSSWDFL 575

Query: 481  EIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYC 540
            EIALLACFC IHNHGF+MLFSFSA+V+H+PS P+GF IL+AGL+KSVLLSVY +  +   
Sbjct: 576  EIALLACFCAIHNHGFDMLFSFSAVVQHSPSTPIGFGILKAGLSKSVLLSVYASPNTRCT 635

Query: 541  HDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVW 600
              N S ER+IASFL +IG+KFLS+YRSCGTYIAFLTILLTV+ V PN ISFGY+ LLL W
Sbjct: 636  QRNASSERRIASFLGAIGQKFLSVYRSCGTYIAFLTILLTVYLVTPNCISFGYILLLLCW 695

Query: 601  MIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASS 660
            +IGRQLVERT+R LWFPLK YAI VF+FIY LS+FS+F MWLS  IDL FYL +NSEA  
Sbjct: 696  IIGRQLVERTRRHLWFPLKFYAITVFIFIYSLSNFSTFEMWLSGLIDLQFYLAYNSEALL 755

Query: 661  LQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALF 720
            LQN WQSLA+LIVMQLYSYERRQS+Y  SD  +L+EF  +GFIKR LIWHSDKI++ A+F
Sbjct: 756  LQNIWQSLAILIVMQLYSYERRQSKYIQSDNAKLMEFGVVGFIKRLLIWHSDKIVYIAVF 815

Query: 721  YASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFP 780
            YAS+S IS FG LYLLGLVIC+ LPK S IPSKLFL YTG+++  EYLFQMWG  AGMFP
Sbjct: 816  YASLSSISLFGFLYLLGLVICSLLPKGSRIPSKLFLLYTGILVMTEYLFQMWGALAGMFP 875

Query: 781  GQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKW 840
            GQ +  L+ FLGF  FQ GFWGLELGLRGKVL+I+ACTLQYNVFRWL+++P   L+K   
Sbjct: 876  GQNNFSLANFLGFELFQSGFWGLELGLRGKVLVISACTLQYNVFRWLQKVPTHILSKDYT 935

Query: 841  DDPCPLFVTEEDDYDISISNEKSKSSSDSGRLFEQQEGL-DHIRRSSFVSGQSQVPHSAS 900
            ++PCPLFV+ E   D S SN +SK  +D   +  + EG+ D++  SS  SG SQ  ++  
Sbjct: 936  EEPCPLFVSAEVFRDFSNSNGESKPPADLPAIPCEAEGVRDNL--SSSASGFSQASNNVP 995

Query: 901  SKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENL 960
             K   S      KYSFG+IWGS  ESHKW++ RII++R+ERFE QK IF +Y+KFWMEN+
Sbjct: 996  -KAGGSAAGIARKYSFGYIWGSTSESHKWNRKRIITMRRERFETQKTIFIVYIKFWMENM 1055

Query: 961  FNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILIL 1020
            FNLFGLEI M++LLLA FALLN++S+ Y+ LLAACILL+R +IRKLWP  VFL ASIL+L
Sbjct: 1056 FNLFGLEINMVALLLACFALLNAISMLYIALLAACILLNRNVIRKLWPALVFLLASILVL 1115

Query: 1021 EYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVF 1080
            EY+  WK+  ++N    SKA VHC DCW+ S+ + ++C +CWLGL +DD  +L SYF+VF
Sbjct: 1116 EYLVIWKSTLSANSQTSSKAEVHCDDCWQSSSSHLRYCESCWLGLAIDDPCVLISYFIVF 1175

Query: 1081 MLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLL 1140
            ML+ LKLRADH S FS S TYR+++SQRKN FVWRDLSFETKSMWT+LDYLRLYCYCHLL
Sbjct: 1176 MLACLKLRADHRSSFSGSYTYREVISQRKNIFVWRDLSFETKSMWTVLDYLRLYCYCHLL 1235

Query: 1141 DLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLA 1200
            DLVLAL+LITGTLEYD+LHLGYLAFALVFFR+RL ILKK+N++FKFLR YNFA+I+LSLA
Sbjct: 1236 DLVLALVLITGTLEYDILHLGYLAFALVFFRMRLTILKKRNRIFKFLRMYNFAVIVLSLA 1295

Query: 1201 YQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQ 1260
            YQSPFVG+  A KC+T++YIFE+IGF+KYDYGFRITARSALVEIIIF+LVS+QSYMFSS 
Sbjct: 1296 YQSPFVGDFCAKKCKTVNYIFEVIGFHKYDYGFRITARSALVEIIIFILVSVQSYMFSSS 1355

Query: 1261 EFEYVCRYLEAEQIGAIVREQEKKAAWKTEQLQHIRD----------------------- 1320
            EF+YV RYLEAEQIG IV EQEKKAAWKT QLQHIR+                       
Sbjct: 1356 EFDYVFRYLEAEQIGTIVSEQEKKAAWKTAQLQHIRECEEKKRQRNTQVEKMKSEMLNLQ 1415

Query: 1321 ------------------SEERKRQRNLQVEKMKSEMLNLQIQLHNMNSFVDGNNV---- 1380
                              SE  +R+R+  +    + M + + +L        G       
Sbjct: 1416 IQLHSGIPTANCDSTSPGSEGLRRRRSSLISNKDAGMWDNENKLLRRQDHAVGEESVFPF 1475

Query: 1381 ----SPSPGNESFRKRSTSGIHDDAMT----TDIEGTPG-------KKKKHKGSAKGNPL 1440
                SP+  N        S  H    +    T+ E   G       KK+K +G AK NP+
Sbjct: 1476 EFYDSPASMNAESLPVMNSADHSFEFSHCEITEAEEDGGGSLYDSDKKEKVRGQAKDNPI 1535

Query: 1441 MSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDD-DDNEQSKTEDRVYDQIESQETRYA 1500
            +SAVQ +GDGVSQVQSIGNQAVS+L +FLN++P+D D NE +  +D  Y+++ESQ   + 
Sbjct: 1536 VSAVQLLGDGVSQVQSIGNQAVSSLVTFLNIMPEDMDANEPTSQDDGKYEEMESQNMGF- 1595

Query: 1501 HLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNFSLL 1560
                + L RSSSLQSDKS+D AS QLGRI  HIW+QM++NND+VCYCCF+LVFLWNFSLL
Sbjct: 1596 ----TDLNRSSSLQSDKSADAASFQLGRILYHIWSQMQSNNDIVCYCCFVLVFLWNFSLL 1655

Query: 1561 SMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLLQEL 1620
            SM YLAALF+YALCVNTGP Y+FWV++LIYTE+YILLQYLYQII+ H   T +S LL EL
Sbjct: 1656 SMAYLAALFLYALCVNTGPTYIFWVIVLIYTEVYILLQYLYQIIVHHHIWTADSGLLHEL 1715

Query: 1621 GFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSSAFN---KNALPSKQSLGH 1680
            GFP H++TS FV+  LPLFLVYLFTL+QSSITAKDGEW   +  N    NAL  K+ L  
Sbjct: 1716 GFPEHKMTSFFVIGILPLFLVYLFTLIQSSITAKDGEWTSYTEINCRRVNALCRKEVLVR 1775

Query: 1681 YGLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGIQPE 1740
              L+ +A ELL     M+   +R+   YWKSL QGAESPPYF+QVS+DV  WP+DGIQPE
Sbjct: 1776 DSLSKKAEELLCHMINMVKMTIRNFFWYWKSLIQGAESPPYFVQVSMDVGSWPDDGIQPE 1835

Query: 1741 RIESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYASPST 1800
            RIESGIN +L+I+H ER  ++NP LC +SSRVHVQSIERS+E T IAL V EV+YASP T
Sbjct: 1836 RIESGINQLLRIVHSERYTDRNPHLCPYSSRVHVQSIERSEEKTNIALAVFEVIYASPPT 1895

Query: 1801 N-TCAEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVFGAD 1860
              T AEW  SLTPA+DVA EI +AQ++  VE  GFPY ILSVIGGGKREIDLYAY+FGAD
Sbjct: 1896 ECTSAEWYRSLTPASDVAKEITIAQQSGSVEEMGFPYIILSVIGGGKREIDLYAYIFGAD 1955

Query: 1861 MIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSFAIG 1920
            + VFFLVAIFYQS+IKN SEFLDV QLEDQFPKEFVF+LMIIFF+IV+DR IYLCS+A+G
Sbjct: 1956 LTVFFLVAIFYQSVIKNKSEFLDVSQLEDQFPKEFVFILMIIFFMIVVDRIIYLCSYALG 2015

Query: 1921 KVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHK 1980
            KVIFYLF+L+LFTY+VTEYAW++ PS Q AG LALRAI++AKAVSL+LQAIQ+RYG+PH+
Sbjct: 2016 KVIFYLFSLILFTYSVTEYAWRLGPSQQQAGVLALRAIYVAKAVSLSLQAIQVRYGIPHQ 2075

Query: 1981 STLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASL 2040
            STLYRQFLTS VSR+NYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDI ASL
Sbjct: 2076 STLYRQFLTSRVSRVNYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDIYASL 2135

Query: 2041 YLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIK 2100
            YLVKCDAVLNR++HKQG+KQT MTKCC+GICLFFILICVIWAPMLMYSSGNPTN+ANPIK
Sbjct: 2136 YLVKCDAVLNRAKHKQGEKQTKMTKCCSGICLFFILICVIWAPMLMYSSGNPTNIANPIK 2195

Query: 2101 DASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQADA 2160
            DAS Q+DIKT  GRLTLYQTTLCEK+ W +LNT++ LDP GYL  ++++DIQLICCQADA
Sbjct: 2196 DASVQIDIKTIRGRLTLYQTTLCEKLPWYELNTDVDLDPEGYLDTFDKNDIQLICCQADA 2255

Query: 2161 SVLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQSDV 2220
            S LWLVP+VVQS F  SL+   D+ I FTW+LTR+RPKGKE+VKY+R ++S+DLPN+ ++
Sbjct: 2256 SELWLVPNVVQSGFSKSLEHDVDMGILFTWVLTRNRPKGKELVKYERPVDSQDLPNRENI 2315

Query: 2221 QKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFHDIQP 2280
            QKVLNGS NGFRI N+Y RYFRVTGSGEVRPLEQE + VSADL++NR    WWSFHDI  
Sbjct: 2316 QKVLNGSSNGFRIHNIYPRYFRVTGSGEVRPLEQEVNAVSADLVMNRAEQPWWSFHDINS 2375

Query: 2281 INVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2331
             +VS CG   GP+A ++SEE PPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQC+DLRM
Sbjct: 2376 SDVSGCGGLKGPMAIIVSEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCADLRM 2435

BLAST of Cucsa.084850 vs. TrEMBL
Match: M5VMP5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000028mg PE=4 SV=1)

HSP 1 Score: 3129.7 bits (8113), Expect = 0.0e+00
Identity = 1609/2399 (67.07%), Postives = 1863/2399 (77.66%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHL-GSHLR 60
            M +QSWKSPSV+YFL++QL  + VA  D++  R GLV   DSCW  F S V  L  SHLR
Sbjct: 96   MILQSWKSPSVLYFLLLQLSVVAVALVDLYGNRFGLVSSCDSCWGRFSSAVERLICSHLR 155

Query: 61   VASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFS 120
            VASCLLLPAIQ++VGIS PSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWW+P  LYAGF+
Sbjct: 156  VASCLLLPAIQLVVGISHPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWKPFHLYAGFN 215

Query: 121  IFLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEE 180
            I L+YVYQLPVE+  ML+W+A+FIGLFKI+ +S+W E+CS++SL+LFYIMLSCVKCDLEE
Sbjct: 216  IVLLYVYQLPVEFSDMLQWIADFIGLFKITLHSDWTEVCSSLSLLLFYIMLSCVKCDLEE 275

Query: 181  MDFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSL 240
            MDFI+SM+E+NL EQLLPSKHSFFIRE RSGV+HTNVLL   VFRTF+INFFTYGFPVSL
Sbjct: 276  MDFILSMKENNLTEQLLPSKHSFFIRESRSGVRHTNVLLTGAVFRTFSINFFTYGFPVSL 335

Query: 241  VALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAF 300
             ALSFWSFHFAS+CAFGLLAYVGYIIYAFPSLF+LHRLNGLLLVFIL WA+STYIFNVAF
Sbjct: 336  FALSFWSFHFASVCAFGLLAYVGYIIYAFPSLFRLHRLNGLLLVFILLWAVSTYIFNVAF 395

Query: 301  TFLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHS 360
             FLN KIGK                       F LGILVAL NLVNNSVFLCLS ED   
Sbjct: 396  AFLNWKIGK-----------------------FCLGILVALGNLVNNSVFLCLSDEDARF 455

Query: 361  SNDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLL 420
            SNDNS+    GETKVLIVATIAWGLRKSSRAI+L LIFL+AMKPGFIHAVYV+FFL    
Sbjct: 456  SNDNSTVEGEGETKVLIVATIAWGLRKSSRAIMLLLIFLIAMKPGFIHAVYVIFFL---- 515

Query: 421  SHDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEF 480
                                  +YLL  ++   +      LC      L ++  + + + 
Sbjct: 516  ----------------------IYLLSHNISRKIRQSLILLCEVHFALLYIIQINPISDT 575

Query: 481  LEIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNY 540
            LE           +   E+L S  AIV+HT S PVGFSIL+AGLNKSVLLSVY ++   Y
Sbjct: 576  LERK--------GSLSAEVL-SQLAIVQHTSSRPVGFSILKAGLNKSVLLSVYASSAIKY 635

Query: 541  CHDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLV 600
             HDNPS+ER+IA FLS+IG+K                  L+V+     YI+F    LL V
Sbjct: 636  SHDNPSYERRIALFLSAIGQK-----------------FLSVYRSCGTYIAF-LTILLTV 695

Query: 601  WMIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEAS 660
            +++    V                      + LSSF S  +WLSR IDL FYLG++SEAS
Sbjct: 696  YLVRPNYVS---------------------FGLSSFRSIEVWLSRLIDLYFYLGYDSEAS 755

Query: 661  SLQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAAL 720
            SL+N W+SLAVLIVMQLYSYERRQSRY+ SD+ ++LEF  LGFIKRF++WHS+KILF A+
Sbjct: 756  SLENVWESLAVLIVMQLYSYERRQSRYNKSDDADVLEFGVLGFIKRFVVWHSNKILFIAV 815

Query: 721  FYASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMF 780
            FYAS+SPIS FG LYLLGLVIC+T PK S IPSKLFL YTG ++T EYLFQMWGRQA MF
Sbjct: 816  FYASLSPISTFGFLYLLGLVICSTFPKASRIPSKLFLVYTGFLVTAEYLFQMWGRQAAMF 875

Query: 781  PGQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGK 840
            PGQKHS +S  LGFR F+PGFWGLE GLRGKVL+IAACTLQYNVFRWLE+MP + LNKGK
Sbjct: 876  PGQKHSNISLLLGFRVFKPGFWGLEFGLRGKVLVIAACTLQYNVFRWLEKMPSTILNKGK 935

Query: 841  WDDPCPLFVTEED-DYDISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSA 900
            W++PCPLFV+ ED + + SI +E++K S+DS  L  ++EG        F  G S+  +  
Sbjct: 936  WEEPCPLFVSAEDANINSSIPSEENKQSTDSEALSVKREGARSHSWPFFSPGLSESHNPM 995

Query: 901  SSKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMEN 960
            S +   SE SS++KYSFG+IWGS KESHKW+K RI++LRKERFE QK+I KIY+KFWMEN
Sbjct: 996  SPRAGGSEGSSSNKYSFGYIWGSTKESHKWNKKRILTLRKERFETQKLISKIYLKFWMEN 1055

Query: 961  LFNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILI 1020
            +FNLFGLEI MI+LLLASFALLN++SL Y+ LLA CI+L+R IIRK+WPI VFLFASILI
Sbjct: 1056 MFNLFGLEINMIALLLASFALLNAISLVYIALLATCIILNRHIIRKIWPILVFLFASILI 1115

Query: 1021 LEYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVV 1080
            LEY A WK+MW SN P  + A   CHDCW+IS  YF +C  CWLGL VDD RML SYF V
Sbjct: 1116 LEYFAIWKSMWPSNHPDETNA--RCHDCWKISTMYFSYCKYCWLGLIVDDPRMLISYFAV 1175

Query: 1081 FMLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHL 1140
            FM +  KLRADHLSGFS+SSTYR+M+SQRKN FVWRDLSFETKSMWT  DYLRLYCYCHL
Sbjct: 1176 FMFACFKLRADHLSGFSVSSTYRQMISQRKNIFVWRDLSFETKSMWTFFDYLRLYCYCHL 1235

Query: 1141 LDLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSL 1200
            LDLVLAL+LITGT+EYD+LHLGYLAFALVFFR+RLEILKK+NK+FKFLR YNFALI+LSL
Sbjct: 1236 LDLVLALVLITGTIEYDILHLGYLAFALVFFRVRLEILKKRNKIFKFLRIYNFALIVLSL 1295

Query: 1201 AYQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSS 1260
            AYQSPFVGE  AGKCET+ YIFEMIG YKYDYGFRITARSALVEI+IFM+VS+QSYMFSS
Sbjct: 1296 AYQSPFVGEFCAGKCETVDYIFEMIGLYKYDYGFRITARSALVEIVIFMVVSLQSYMFSS 1355

Query: 1261 QEFEYVCRYLEAEQIGA-----------------IVREQEKKAAWKTEQLQHIRD----- 1320
            QEF+ V RYLEAEQIGA                  +RE E+K   +  Q++ ++      
Sbjct: 1356 QEFDNVSRYLEAEQIGAIVREQEKKAAWKTAQLKHIRESEEKKHQRNLQVEKMKSEMLNL 1415

Query: 1321 -------------------SEERKRQRNLQVEK-------------MKSEMLNLQ----- 1380
                               SE  +R+R+  +               MK E +  +     
Sbjct: 1416 QIQLHSMNSVTNCGDSPPVSEGLRRRRSTSLNSNNDAGTPDKEGLPMKKEQILKEDSLYP 1475

Query: 1381 IQLHNMNSFVDGNNV----SPSPGNESFRKRSTSGIHDDAMTTDIEGTPGKKKKHKGSAK 1440
             +LH   + V+  N     S     ESF    T    +D     +  +  KK+K KG AK
Sbjct: 1476 YELHQSPATVNMENPTVVESMKDSMESFHCEITEV--EDVTDGVLFYSSEKKEKVKGQAK 1535

Query: 1441 GNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDDNEQSKTEDRVYDQIESQET 1500
             +PL+SAV  IGDGVSQVQSIGNQAV+NL SFLN+  + D NE S  ED VYD++ESQ T
Sbjct: 1536 ESPLISAVHLIGDGVSQVQSIGNQAVNNLVSFLNIEQESDINEHSSVEDGVYDEMESQNT 1595

Query: 1501 RYAHLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNF 1560
            +Y    RS     SSLQSD SSDP S+QLGRIFRHIW+QMR+NND+VCYCCF++VFLWNF
Sbjct: 1596 KYMCFNRS-----SSLQSDTSSDPTSLQLGRIFRHIWSQMRSNNDIVCYCCFVIVFLWNF 1655

Query: 1561 SLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLL 1620
            SLLSM YLAALF+YALCVN+GP Y+FWV+MLIYTE+YILLQYLYQIIIQH GL++ SDLL
Sbjct: 1656 SLLSMVYLAALFLYALCVNSGPSYIFWVIMLIYTEVYILLQYLYQIIIQHWGLSVASDLL 1715

Query: 1621 QELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSSAFN---KNALPSKQS 1680
            +E GFP H+ITSSFVVSSLPLFLVYLFTL+QSSITAKDGEW  S+ F+   ++A   K+ 
Sbjct: 1716 REWGFPAHKITSSFVVSSLPLFLVYLFTLIQSSITAKDGEWMSSTDFDFYRRSAFHGKEV 1775

Query: 1681 LGHYGLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGI 1740
               Y  +++  ELL+I    +  ++RS  +YW+SLTQGA+SPPYFIQVS+DV+ WP+DGI
Sbjct: 1776 PVSYSWSEKTKELLHIMGNAIKLIIRSFFRYWESLTQGADSPPYFIQVSMDVRSWPDDGI 1835

Query: 1741 QPERIESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYAS 1800
            QPERIESG+N +L+IIH ERCK++ P  C F+SRVHVQSIERS+EN  +ALVV EVVYAS
Sbjct: 1836 QPERIESGVNQLLRIIHDERCKQKTPTPCPFASRVHVQSIERSQENANVALVVFEVVYAS 1895

Query: 1801 PSTNTCA-EWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVF 1860
            P T   + EW +SLTPAADVA EIL AQ   FVE  GFPY ILSVIGGGKR++DLYAYVF
Sbjct: 1896 PITECASVEWYNSLTPAADVAKEILQAQNAGFVEEIGFPYPILSVIGGGKRDVDLYAYVF 1955

Query: 1861 GADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSF 1920
            GAD+ VFFLVAIFYQS+IKN SEFLDVYQLEDQFPKEFVF+LMIIFFLIVLDR IYLCSF
Sbjct: 1956 GADLTVFFLVAIFYQSVIKNKSEFLDVYQLEDQFPKEFVFILMIIFFLIVLDRIIYLCSF 2015

Query: 1921 AIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGL 1980
            A GKVIFYLFNL+LFTY+VTEYAW MEPS+QHAG LALRAIFLAKAVSLALQAIQ+R+G+
Sbjct: 2016 ATGKVIFYLFNLILFTYSVTEYAWHMEPSHQHAGGLALRAIFLAKAVSLALQAIQLRHGI 2075

Query: 1981 PHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDIN 2040
            PHKSTLYRQFLTS++SRINYLGYRLYRALPFLYELRC LDWSCTTTSLTMYDWLKLEDI+
Sbjct: 2076 PHKSTLYRQFLTSEISRINYLGYRLYRALPFLYELRCALDWSCTTTSLTMYDWLKLEDIH 2135

Query: 2041 ASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVAN 2100
            ASLYLVKCDAVLNR++HKQG+KQT MTKCCNGICLFFILICVIWAPMLMYSSGNPTN+ N
Sbjct: 2136 ASLYLVKCDAVLNRAKHKQGEKQTKMTKCCNGICLFFILICVIWAPMLMYSSGNPTNIEN 2195

Query: 2101 PIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQ 2160
            PIKDAS QVDIKT SGRL+LYQTTLC+K+ WDKLN+++ LDP GYL  YNQ D+QLICC+
Sbjct: 2196 PIKDASVQVDIKTASGRLSLYQTTLCKKLQWDKLNSDVNLDPKGYLDTYNQKDVQLICCE 2255

Query: 2161 ADASVLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQ 2220
            ADAS LWL+P+VVQ+RF+ SLD    + ISFTW+L+R RPKGKEVVKY+R ++ +DLP Q
Sbjct: 2256 ADASTLWLIPNVVQTRFIQSLDWDTHMDISFTWVLSRGRPKGKEVVKYERSVDPQDLPKQ 2315

Query: 2221 SDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFHD 2280
            SDVQKVLNGS+N FRI NVY RYFRVTGSG+VRPLE E++FVSADL++NR NY WWSFHD
Sbjct: 2316 SDVQKVLNGSINSFRIYNVYSRYFRVTGSGDVRPLELEDNFVSADLVINRANYIWWSFHD 2375

Query: 2281 IQPINVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSD 2331
            I   +V+ CG   GP+A ++SEE PP+GILGDTLSKFSIWGLYITFVLAVGRFIRLQCSD
Sbjct: 2376 INSSDVNGCGGLRGPMAIIVSEETPPEGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSD 2388

BLAST of Cucsa.084850 vs. TAIR10
Match: AT2G48060.1 (AT2G48060.1 unknown protein)

HSP 1 Score: 1859.7 bits (4816), Expect = 0.0e+00
Identity = 925/1372 (67.42%), Postives = 1099/1372 (80.10%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRV 60
            M ++SW++P+V+YFL +QLL   VA ADI+  R G     D+ W HF     HLGSHLRV
Sbjct: 96   MILKSWRNPTVMYFLALQLLTSLVALADIYSSRFGFARWRDTWWSHFSGIFEHLGSHLRV 155

Query: 61   ASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSI 120
            ASCLLLPA+Q+ VGI  PSWVSLPFFIGSC GLVDWSLTSN  GLFRWWR L +YAGF+I
Sbjct: 156  ASCLLLPAVQLAVGICNPSWVSLPFFIGSCAGLVDWSLTSNVSGLFRWWRVLYIYAGFNI 215

Query: 121  FLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEM 180
             L+Y+YQLP+ +  M++W+A FIGLF+IS  +E P+ICS + L+LFYIMLS V+ DLE+M
Sbjct: 216  VLLYLYQLPINFSDMIRWIASFIGLFRISLETEGPDICSGLFLVLFYIMLSYVRSDLEDM 275

Query: 181  DFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV 240
            DFIMS  E+NL E+LLP K+SFFIRE R+GV+HTNVLLR  VF+TF+INFFTYGFPVSL 
Sbjct: 276  DFIMSTSENNLAERLLPPKYSFFIRESRAGVRHTNVLLRGAVFKTFSINFFTYGFPVSLF 335

Query: 241  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFT 300
            ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFIL WA+STYIFNVAF+
Sbjct: 336  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILLWAVSTYIFNVAFS 395

Query: 301  FLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSS 360
            FLN K+GK                       FGLG+LVAL NLVNNSVFL LS E   SS
Sbjct: 396  FLNTKVGK-----------------------FGLGMLVALGNLVNNSVFLYLSEESSRSS 455

Query: 361  NDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLS 420
            N+ S      ETKVL+VATIAWGLRK SRAI+L LIFL+AMKPGF HAVYV+FFL+YLLS
Sbjct: 456  NERSYVEADEETKVLVVATIAWGLRKCSRAIMLALIFLIAMKPGFFHAVYVIFFLMYLLS 515

Query: 421  HDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFL 480
            H+++RK+R+SLILL  VHFALLY+L+I L+S+ L +EGS  RE+L QLGLL  +S W+FL
Sbjct: 516  HNINRKIRKSLILLCEVHFALLYILEIDLVSNSLKQEGSASREVLFQLGLLRSESSWDFL 575

Query: 481  EIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYC 540
            EIALLACFC IHNHGFE+LFSFSAIV HTPSPP+GFSIL+AGLNKSVLLSVY++ +S+Y 
Sbjct: 576  EIALLACFCAIHNHGFEVLFSFSAIVRHTPSPPIGFSILKAGLNKSVLLSVYSSPSSSYS 635

Query: 541  HDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVW 600
             DN ++ER IASFLS+IG+KFLSMYRSCGTYIAF+TIL++V+ VKPNY+SFGY+FLLL+W
Sbjct: 636  QDNTTYERHIASFLSAIGQKFLSMYRSCGTYIAFITILISVYLVKPNYVSFGYIFLLLLW 695

Query: 601  MIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASS 660
            + GRQL E TKRRLWFPLKAYA++VF+FIYCLSSF S ++WLS  IDL FYLG+NS+A  
Sbjct: 696  ITGRQLFEETKRRLWFPLKAYAVLVFMFIYCLSSFVSLQLWLSGFIDLYFYLGYNSKAPL 755

Query: 661  LQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALF 720
            L N W+SLAVLIVMQLYSYERRQS +    +  LL     GF +RFL WH  KILFAALF
Sbjct: 756  LDNVWESLAVLIVMQLYSYERRQSGHYIPGQSSLLHPGVFGFFERFLAWHGQKILFAALF 815

Query: 721  YASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFP 780
            YAS+SPIS FG +YLLGLVIC T PK S IPSK FL YTG +++ EYLFQ+WG QA MFP
Sbjct: 816  YASLSPISVFGFVYLLGLVICTTFPKSSSIPSKSFLIYTGFLVSAEYLFQLWGMQAQMFP 875

Query: 781  GQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKW 840
            GQK++ LS++LG R ++PGFWG+E GLRGKVL++AACTLQYNVFRWLER  G  + KGK+
Sbjct: 876  GQKYAELSFYLGLRVYEPGFWGIESGLRGKVLVVAACTLQYNVFRWLERTSGLTVIKGKY 935

Query: 841  DDPCPLFVTEED-DYDISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSAS 900
            ++PCPLFV+ ED    +S SN ++ SS+D   +  +Q          F    +Q      
Sbjct: 936  EEPCPLFVSAEDTTASVSSSNGENPSSTDHASISMKQGEATSNSWPFFSPRGNQGAGFLH 995

Query: 901  SKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENL 960
             K   SE  S+ K+SFG  WGSIKESH+W++ RI++L+KERFE QK + KIY+KFW+EN+
Sbjct: 996  PKTGGSESGSSRKFSFGHFWGSIKESHRWNRRRILALKKERFETQKNLLKIYLKFWIENM 1055

Query: 961  FNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILIL 1020
            FNL+GLEI MI+LLLASFALLN++S+ Y+ LLAAC+LL R +I+KLWP+ VFLFASIL +
Sbjct: 1056 FNLYGLEINMIALLLASFALLNAISMVYIALLAACVLLRRRVIQKLWPVVVFLFASILAI 1115

Query: 1021 EYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVF 1080
            EY+A W +   S+   PS+  VHCHDCW I+  YF+FC  CWLG+ VDD R L SYFVVF
Sbjct: 1116 EYVATWNSFLPSD-QAPSETSVHCHDCWSIAALYFKFCRECWLGVRVDDPRTLISYFVVF 1175

Query: 1081 MLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLL 1140
            ML+  KLRADH+S FS SSTY +M SQRKN+FVWRDLSFETKSMWT+LDYLRLYCY HLL
Sbjct: 1176 MLACFKLRADHISSFSESSTYHQMKSQRKNSFVWRDLSFETKSMWTVLDYLRLYCYVHLL 1235

Query: 1141 DLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLA 1200
            D+VL LILITGTLEYD+LHLGYLAFALVF R+RLEILKKKNK+F+FLR YNF LII SLA
Sbjct: 1236 DVVLILILITGTLEYDILHLGYLAFALVFARMRLEILKKKNKIFRFLRVYNFVLIIFSLA 1295

Query: 1201 YQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQ 1260
            YQSPFVG  + GKCET+ YI+E+IGFYKYDYGFRITARSALVEIIIFMLVS+QSYMFSSQ
Sbjct: 1296 YQSPFVGNFNDGKCETVDYIYEVIGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQ 1355

Query: 1261 EFEYVCRYLEAEQIGAIVREQEKKAAWKTEQLQHIRDSEERKRQRNLQVEKMKSEMLNLQ 1320
            EF+YV RYLEAEQIGAIVREQEKKAA KTEQLQ IR++EE+KRQRNLQVEKMKSEMLNL+
Sbjct: 1356 EFDYVSRYLEAEQIGAIVREQEKKAARKTEQLQQIREAEEKKRQRNLQVEKMKSEMLNLR 1415

Query: 1321 IQLHNMNSFVDGNNVSPSPGNESFRKRSTSGIHDD--AMTTDIEGTPGKKKK 1370
            +QLH MNS  D N    SP  E  R+R +  +  D  A + +I+G   +K++
Sbjct: 1416 VQLHRMNS--DSNFGVASPRTEGLRRRKSPYLIPDSGAASPEIDGVVHRKEE 1441

HSP 2 Score: 1412.1 bits (3654), Expect = 0.0e+00
Identity = 688/972 (70.78%), Postives = 815/972 (83.85%), Query Frame = 1

Query: 1366 KKKKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDD-NEQSKTED 1425
            +++K K   K NPL+SAVQ IGDGVSQVQ IGNQAV+NL +FLN+ P++ D NEQS  +D
Sbjct: 1494 RERKQKSEGKENPLISAVQLIGDGVSQVQFIGNQAVNNLVNFLNISPENSDTNEQSSVDD 1553

Query: 1426 RVYDQIESQETRYAHLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCY 1485
             VYD++ESQ+ ++     +  ERS+SLQSD+SSD  S Q+GRIFRHIW++M++NND+VCY
Sbjct: 1554 EVYDEMESQKRKH-----TPFERSTSLQSDRSSDGTSFQIGRIFRHIWSRMQSNNDIVCY 1613

Query: 1486 CCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQ 1545
            CCFI+ FLWNFSLLSM YLAALF+YALCV+TGP ++FWV+ML+YTE+YILLQYLYQIIIQ
Sbjct: 1614 CCFIIAFLWNFSLLSMVYLAALFLYALCVHTGPTHIFWVIMLMYTEIYILLQYLYQIIIQ 1673

Query: 1546 HCGLTINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSSAFN- 1605
            HCGL+I++ LL ELGFPT RI SSFVVSSLPLFL+Y+FTL+QSSIT KDG+W  S+ F  
Sbjct: 1674 HCGLSIDAPLLHELGFPTQRIKSSFVVSSLPLFLIYIFTLIQSSITVKDGDWVPSADFTS 1733

Query: 1606 -KNALPSKQSLGHYGLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSL 1665
             +NA  S++ L    L+ R  ++    R     V+RS+ +YW SLT+GAESPPYF+QV++
Sbjct: 1734 RRNARGSQKDLTRIRLSQRILDVFKKLRDSAKLVIRSIYRYWISLTRGAESPPYFVQVTM 1793

Query: 1666 DVQIWPEDGIQPERIESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIA 1725
            DV +WPEDGIQPER+E  +N +L+++H ERC++ NP LC +SSRVHVQSIERS E    A
Sbjct: 1794 DVHMWPEDGIQPERVECRMNQLLRLVHNERCEKGNPDLCPYSSRVHVQSIERSTETPNEA 1853

Query: 1726 LVVLEVVYASPSTNTC--AEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGG 1785
            LVVLEV YASP TN C  AEW  SLTPA+DVA EI  AQ +   E TGFPY ILSVIGGG
Sbjct: 1854 LVVLEVEYASP-TNGCSSAEWYKSLTPASDVAKEIRKAQHSGLGEGTGFPYPILSVIGGG 1913

Query: 1786 KREIDLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLI 1845
            KR+ DLYAY+FGAD+IVFFLVAIFYQS+IKN SEF+DVYQLEDQFP +FV +LM+IFFLI
Sbjct: 1914 KRDTDLYAYIFGADLIVFFLVAIFYQSVIKNKSEFIDVYQLEDQFPFDFVIILMVIFFLI 1973

Query: 1846 VLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSL 1905
            V+DR IYLCSFA GKV++YLF+L+LFTYAVTEYAW + P+ QHA  LALR IFLAKA+SL
Sbjct: 1974 VVDRVIYLCSFATGKVVYYLFSLILFTYAVTEYAWSIYPTQQHAAGLALRIIFLAKAMSL 2033

Query: 1906 ALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLT 1965
            ALQAIQIRYGLPHKSTLYRQFLTS+VSRINY GYRLYRALPFLYELRCVLDWSCT TSLT
Sbjct: 2034 ALQAIQIRYGLPHKSTLYRQFLTSEVSRINYYGYRLYRALPFLYELRCVLDWSCTATSLT 2093

Query: 1966 MYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLM 2025
            MYDWLKLED+NASLYLVKCD VLNR+ HK G+KQT MTKCCNGICLFFIL+CVIWAPMLM
Sbjct: 2094 MYDWLKLEDVNASLYLVKCDTVLNRATHKHGEKQTKMTKCCNGICLFFILLCVIWAPMLM 2153

Query: 2026 YSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPY 2085
            YSSGNPTN+ANPIKDAS Q+D+KT  G+LTLYQTTLCE+IS D ++  + L    +L  Y
Sbjct: 2154 YSSGNPTNIANPIKDASVQIDLKTVGGKLTLYQTTLCERISGDNIDLGLDLGSQSFLPTY 2213

Query: 2086 NQDDIQLICCQADASVLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYD 2145
            N++DIQLICCQADASVLWLVPD V +RF+ SLD   D+ I+FTW+L RDRPKGKE VKY+
Sbjct: 2214 NKNDIQLICCQADASVLWLVPDTVVTRFIQSLDWDTDMDITFTWVLNRDRPKGKETVKYE 2273

Query: 2146 RVIESRDLPNQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILN 2205
            R ++  DLP +SD+Q VLNGSM+GFR+ N+Y ++FRVTGSG+VR  E +   VSAD+++N
Sbjct: 2274 RSVDPLDLPKRSDIQMVLNGSMDGFRVHNLYPKFFRVTGSGDVRSFEDQTDEVSADILIN 2333

Query: 2206 RNNYE-WWSFHDIQPI-NVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFV 2265
              N++ WWSFH+++   N+S C    GPVA ++SEE PPQG LGDTLSKFSIWGLYITFV
Sbjct: 2334 HANFKWWWSFHNLKASENISACEGMDGPVAIIMSEETPPQGFLGDTLSKFSIWGLYITFV 2393

Query: 2266 LAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYWTLVKIYR 2325
            LAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICED+YAARAEGELGVEEVLYWTLVKIYR
Sbjct: 2394 LAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDLYAARAEGELGVEEVLYWTLVKIYR 2453

Query: 2326 SPHMLLEYTKVD 2331
            SPHMLLEYTK+D
Sbjct: 2454 SPHMLLEYTKLD 2459

BLAST of Cucsa.084850 vs. NCBI nr
Match: gi|645258836|ref|XP_008235072.1| (PREDICTED: uncharacterized protein LOC103333938 [Prunus mume])

HSP 1 Score: 3474.5 bits (9008), Expect = 0.0e+00
Identity = 1726/2399 (71.95%), Postives = 1990/2399 (82.95%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHL-GSHLR 60
            M +QSWKSPSV+YFL++QL  + VA  D++  R GLV   DSCW  F S V  L  SHLR
Sbjct: 96   MILQSWKSPSVLYFLLLQLSVVAVALVDLYGNRFGLVSSCDSCWGRFSSAVERLICSHLR 155

Query: 61   VASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFS 120
            VASCLLLPAIQ++VGIS PSWVSLPFFIGSC GLVDWSLTSNFLGLFRWW+P  LYAGF+
Sbjct: 156  VASCLLLPAIQLVVGISHPSWVSLPFFIGSCSGLVDWSLTSNFLGLFRWWKPFHLYAGFN 215

Query: 121  IFLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEE 180
            I L+YVYQLPVE+P ML+W+A+FIGLFKI+ +S+W E+CS++SL+LFYIMLSCVKCDLEE
Sbjct: 216  IVLLYVYQLPVEFPDMLQWIADFIGLFKITLHSDWTEVCSSLSLLLFYIMLSCVKCDLEE 275

Query: 181  MDFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSL 240
            MDFI+SM+E+NL EQLLPSKHSFFIRE RSGV+HTNVLL   VFRTF+INFFTYGFPVSL
Sbjct: 276  MDFILSMKENNLTEQLLPSKHSFFIRESRSGVRHTNVLLTGAVFRTFSINFFTYGFPVSL 335

Query: 241  VALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAF 300
             ALSFWSFHFAS+CAFGLLAYVGYIIYAFPSLF+LHRLNGLLLVFIL WA+STYIFNVAF
Sbjct: 336  FALSFWSFHFASVCAFGLLAYVGYIIYAFPSLFRLHRLNGLLLVFILLWAVSTYIFNVAF 395

Query: 301  TFLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHS 360
             FLN KIGK+ D+WEMVGLWHYPIPGFFLLAQF LGILVAL NLVNNSVFLCLS ED   
Sbjct: 396  AFLNWKIGKNTDIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDEDGRF 455

Query: 361  SNDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLL 420
             NDNS+    GETKVLIVATIAWGLRKSSRAI+L LIFL+AMKPGFIHAVYV+FFL+YLL
Sbjct: 456  LNDNSTVEGEGETKVLIVATIAWGLRKSSRAIMLALIFLIAMKPGFIHAVYVIFFLIYLL 515

Query: 421  SHDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEF 480
            SH++SRK+RQ+LILL  VHFALLY++QI+ IS  L+R+GSL  E+L QLGLL  +S W+F
Sbjct: 516  SHNISRKIRQALILLCEVHFALLYIIQINPISDALERKGSLSAEVLSQLGLLQHESSWDF 575

Query: 481  LEIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNY 540
            L+IALLACFC IHNHGFEMLFSFSAIV+HTPS PVGFSIL+AGLNKSVLLSVY ++   Y
Sbjct: 576  LKIALLACFCAIHNHGFEMLFSFSAIVQHTPSRPVGFSILKAGLNKSVLLSVYASSAIKY 635

Query: 541  CHDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLV 600
             HDNPS+E++IA FLS+IG++FLS+YRSCGTYIAFLTILLTV+ V+PNY+SFGY+FLLL 
Sbjct: 636  SHDNPSYEKRIALFLSAIGQRFLSVYRSCGTYIAFLTILLTVYLVRPNYVSFGYIFLLLA 695

Query: 601  WMIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEAS 660
            W+IGRQLVERTK+RLWFPLKAYAI+VF+FIY LSSF    +WLSR IDL FYLG++SEAS
Sbjct: 696  WIIGRQLVERTKKRLWFPLKAYAIVVFIFIYSLSSFRCIEVWLSRLIDLYFYLGYDSEAS 755

Query: 661  SLQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAAL 720
            SL+N W+SLAVLIVMQLYSYERRQSRY+  D+ ++LEF  LGFIKRF++WHS+KILF A+
Sbjct: 756  SLENVWESLAVLIVMQLYSYERRQSRYNKPDDADVLEFGVLGFIKRFVVWHSNKILFIAV 815

Query: 721  FYASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMF 780
            FYAS+SPISAFG LYLLGLVIC+TLPK S IPSKLFL YTG ++T EYLFQMWGRQA MF
Sbjct: 816  FYASLSPISAFGFLYLLGLVICSTLPKASRIPSKLFLVYTGFLVTAEYLFQMWGRQAAMF 875

Query: 781  PGQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGK 840
            PGQKHS +S  LGFR F+PGFWGLE GLRGKVL+IAACTLQYNVFRWLE+MP + LNKGK
Sbjct: 876  PGQKHSNISLLLGFRVFKPGFWGLEFGLRGKVLVIAACTLQYNVFRWLEKMPSTILNKGK 935

Query: 841  WDDPCPLFVTEED-DYDISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSA 900
            W++PCPLFV+ ED   + SI +E++K S+DS  L  ++EG        F  G S+  +  
Sbjct: 936  WEEPCPLFVSAEDAKINSSIPSEENKPSTDSEALSVKREGARSHSWPFFSPGLSESLNPV 995

Query: 901  SSKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMEN 960
            S +   SE SS++KYSFG+IWGS KESHKW+K RI++LRKERFE QK+I KIY+KFWMEN
Sbjct: 996  SPRAGGSEGSSSNKYSFGYIWGSTKESHKWNKKRILALRKERFETQKLISKIYLKFWMEN 1055

Query: 961  LFNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILI 1020
            +FNLFGLEI MI+LLLASFALLN++SL Y+ LLA CI+L+R IIRK+WPI VFLFASILI
Sbjct: 1056 MFNLFGLEINMIALLLASFALLNAISLVYIALLATCIILNRHIIRKIWPILVFLFASILI 1115

Query: 1021 LEYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVV 1080
            LEY A WK+ W SN P  + A   CHDCW+IS  YF +C  CWLGL VDD RML SYF V
Sbjct: 1116 LEYFAIWKSTWPSNHPDETNA--RCHDCWKISTMYFSYCKYCWLGLIVDDPRMLISYFAV 1175

Query: 1081 FMLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHL 1140
            FM +  KLRADHLSGFS+SSTYR+M+SQRKN FVWRDLSFETKSMWT  DYLRLYCYCHL
Sbjct: 1176 FMFACFKLRADHLSGFSVSSTYRQMVSQRKNIFVWRDLSFETKSMWTFFDYLRLYCYCHL 1235

Query: 1141 LDLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSL 1200
            LDLVLAL+LITGT+EYD+LHLGYLAFALVFFR+RLEILKK+NK+FKFLR YNFALI+LSL
Sbjct: 1236 LDLVLALVLITGTIEYDILHLGYLAFALVFFRVRLEILKKRNKIFKFLRIYNFALIVLSL 1295

Query: 1201 AYQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSS 1260
            AYQSPFVGE  AGKCET+ YIFEMIGFYKYDYGFRITARSALVEI+IFM+VS+QSYMFSS
Sbjct: 1296 AYQSPFVGEFCAGKCETVDYIFEMIGFYKYDYGFRITARSALVEIVIFMVVSLQSYMFSS 1355

Query: 1261 QEFEYVCRYLEAEQIGA-----------------IVREQEKKAAWKTEQLQHIRD----- 1320
            QEF+ V RYLEAEQIGA                  +RE E+K   +  Q++ ++      
Sbjct: 1356 QEFDNVSRYLEAEQIGAIVREQEKKAAWKTAQLKHIRESEEKKHQRNLQVEKMKSEMLNL 1415

Query: 1321 -------------------SEERKRQRNLQVEK-------------MKSEMLNLQ----- 1380
                               SE  +R+R+  +               MK E +  +     
Sbjct: 1416 QIQLHSMNSVTNCGDSPPVSEGLRRRRSTSLNSNNDAGTPDKEGLPMKKEQILKEDSLYP 1475

Query: 1381 IQLHNMNSFVDGNNV----SPSPGNESFRKRSTSGIHDDAMTTDIEGTPGKKKKHKGSAK 1440
             +LH   + V+  N     S     ESF    T  + +D     +  +  KK K KG AK
Sbjct: 1476 YELHRSPATVNLENPTVVESMKDSMESFHCEITE-VEEDVTDGVLFYSSEKKDKVKGQAK 1535

Query: 1441 GNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDDNEQSKTEDRVYDQIESQET 1500
             +PL+SAV  IGDGVSQVQSIGNQAV+NL SFLN+  + D +E S  ED VYD++ESQ T
Sbjct: 1536 ESPLISAVHLIGDGVSQVQSIGNQAVNNLVSFLNIEQESDISEHSSVEDGVYDEMESQNT 1595

Query: 1501 RYAHLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNF 1560
            +Y    RS     SSLQSD SSDP S+QLGRIFRHIW+QMR+NND+VCYCCF++VFLWNF
Sbjct: 1596 KYMCFNRS-----SSLQSDTSSDPTSLQLGRIFRHIWSQMRSNNDIVCYCCFVIVFLWNF 1655

Query: 1561 SLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLL 1620
            SLLSM YLAALF+YALCVN+GP Y+FWV+MLIYTE+YILLQYLYQIIIQH GL++ SDLL
Sbjct: 1656 SLLSMVYLAALFLYALCVNSGPSYIFWVIMLIYTEVYILLQYLYQIIIQHWGLSVASDLL 1715

Query: 1621 QELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSSAFN---KNALPSKQS 1680
            +E GFP H+ITSSFVVSSLPLFLVYLFTL+QSSITAKDGEW  S+ F+   ++A   K+ 
Sbjct: 1716 REWGFPAHKITSSFVVSSLPLFLVYLFTLIQSSITAKDGEWMSSTDFDFYRRSAFHGKEV 1775

Query: 1681 LGHYGLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGI 1740
               Y  +++  ELL+I    +  ++RS  +YW+SLTQGA+SPPYFIQVS+DV+ WP+DGI
Sbjct: 1776 PVSYSWSEKTKELLHIMGNAIKLIIRSFFRYWESLTQGADSPPYFIQVSMDVRSWPDDGI 1835

Query: 1741 QPERIESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYAS 1800
            QPERIESG+N +L+IIH ERCK++ P  C F+SRVHVQSIERS+EN  +ALVV EVVYAS
Sbjct: 1836 QPERIESGVNQLLRIIHDERCKQKTPTPCPFASRVHVQSIERSQENENVALVVFEVVYAS 1895

Query: 1801 PSTNTCA-EWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVF 1860
            P T   + EW +SLTPAADVA EIL AQ   FVE  GFPY ILSVIGGGKR++DLYAYVF
Sbjct: 1896 PVTECASVEWYNSLTPAADVAKEILKAQHAGFVEEIGFPYPILSVIGGGKRDVDLYAYVF 1955

Query: 1861 GADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSF 1920
            GAD+ VFFLVAIFYQS+IKN SEFLDVYQL+DQFPKEFVF+LMIIFFLIVLDR IYLCSF
Sbjct: 1956 GADLTVFFLVAIFYQSVIKNKSEFLDVYQLDDQFPKEFVFILMIIFFLIVLDRIIYLCSF 2015

Query: 1921 AIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGL 1980
            A GKVIFYLFNL+LFTY+VTEYAW MEPS+QHAG LALRAIFLAKAVSLALQAIQ+R+G+
Sbjct: 2016 ATGKVIFYLFNLILFTYSVTEYAWHMEPSHQHAGGLALRAIFLAKAVSLALQAIQLRHGI 2075

Query: 1981 PHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDIN 2040
            PHKSTLYRQFLTS++SRINYLGYRLYRALPFLYELRC LDWSCTTTSLTMYDWLKLEDI+
Sbjct: 2076 PHKSTLYRQFLTSEISRINYLGYRLYRALPFLYELRCALDWSCTTTSLTMYDWLKLEDIH 2135

Query: 2041 ASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVAN 2100
            ASLYLVKCDAVLNR++HKQG+KQT MTKCCNGICLFFILICVIWAPMLMYSSGNPTN+ N
Sbjct: 2136 ASLYLVKCDAVLNRAKHKQGEKQTKMTKCCNGICLFFILICVIWAPMLMYSSGNPTNIEN 2195

Query: 2101 PIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQ 2160
            PIKDAS QVDIKT SGRL+LYQTTLC+K+ WD LN+++ LDP GYL  YNQ D+QLICC+
Sbjct: 2196 PIKDASVQVDIKTASGRLSLYQTTLCKKLQWDTLNSDVNLDPKGYLDTYNQKDVQLICCE 2255

Query: 2161 ADASVLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQ 2220
            ADAS LWL+P VVQ+RF+ SLD +  + ISFTW+LTR RPKGKEVVKY+R ++ +DLP Q
Sbjct: 2256 ADASTLWLIPSVVQTRFIQSLDWETHMDISFTWVLTRGRPKGKEVVKYERSVDPQDLPKQ 2315

Query: 2221 SDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFHD 2280
            SDVQ+VLNGS+N FRI NVY RYFRVTGSG+VRPLE E++FVSADL++NR NY+WWSFHD
Sbjct: 2316 SDVQQVLNGSINSFRIYNVYSRYFRVTGSGDVRPLELEDNFVSADLVINRANYDWWSFHD 2375

Query: 2281 IQPINVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSD 2331
            I   +V+ CG   GP+A ++SEE PPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSD
Sbjct: 2376 INSSDVNGCGGLRGPMAIIVSEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSD 2435

BLAST of Cucsa.084850 vs. NCBI nr
Match: gi|658061383|ref|XP_008366559.1| (PREDICTED: uncharacterized protein LOC103430200 [Malus domestica])

HSP 1 Score: 3419.0 bits (8864), Expect = 0.0e+00
Identity = 1712/2400 (71.33%), Postives = 1971/2400 (82.12%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHL-GSHLR 60
            M +QSWKSP V+YFL++QL  + VA  D++  R GLV   DSCW  F S V  L  SHLR
Sbjct: 96   MILQSWKSPFVLYFLLLQLSVVAVALVDLYGNRFGLVSSCDSCWGRFSSAVERLICSHLR 155

Query: 61   VASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFS 120
            VA  LLLPAIQ++VGIS PSWVSLPFFIGSC+GLVDWSLTSNFLGLFRWW+P  LYAGF+
Sbjct: 156  VAVLLLLPAIQLVVGISHPSWVSLPFFIGSCIGLVDWSLTSNFLGLFRWWKPFHLYAGFN 215

Query: 121  IFLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEE 180
            I L+YVYQLPVE+P ML+WVA+FIGLFKI+ +S+W E+CS+ SL+LFY MLS VKCDLEE
Sbjct: 216  IVLLYVYQLPVEFPDMLQWVAKFIGLFKITLHSDWTEVCSSCSLLLFYTMLSYVKCDLEE 275

Query: 181  MDFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSL 240
            MD I+SM+E+NL+EQLLPSKHSFFIRE RSGV+HTNVLL   VFRTF+INFFTYGFPVSL
Sbjct: 276  MDCILSMKENNLMEQLLPSKHSFFIRESRSGVRHTNVLLTGAVFRTFSINFFTYGFPVSL 335

Query: 241  VALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAF 300
             ALSFWSFHFAS+CAFGLLAYVGYIIY FPSLF+LHRLNGLLLVFIL WA STYIFNVAF
Sbjct: 336  FALSFWSFHFASICAFGLLAYVGYIIYVFPSLFRLHRLNGLLLVFILLWAASTYIFNVAF 395

Query: 301  TFLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHS 360
             FLNRKIGK+MD+WEM+GLWHYPIPGFFLLAQF LGILVAL NLVNNSVFL LS ED  S
Sbjct: 396  AFLNRKIGKNMDIWEMIGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLWLSDEDGQS 455

Query: 361  SNDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLL 420
            SNDNS+    GETKVLIVATIAWGLRKSSRAI+L LI L+AMKPGFIHAVYV+FFL+YLL
Sbjct: 456  SNDNSTAEGEGETKVLIVATIAWGLRKSSRAIMLALILLIAMKPGFIHAVYVIFFLIYLL 515

Query: 421  SHDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEF 480
            SH++SRKMR+SLILL  VHFALLY++QI+ IS  L+++GSL  EIL QLGL+  +S W+F
Sbjct: 516  SHNISRKMRKSLILLCEVHFALLYIIQINPISDTLEQKGSLSAEILSQLGLVQHESSWDF 575

Query: 481  LEIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNY 540
            L+IALLACFC IHNHGFEMLFSFSAIV+HTPS P GFSIL+AGLNKSVLLSVY ++   Y
Sbjct: 576  LQIALLACFCAIHNHGFEMLFSFSAIVQHTPSRPFGFSILKAGLNKSVLLSVYASSAIQY 635

Query: 541  CHDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLV 600
             HDNPS+ER+IA FLS+IG+KFLS+YRSCGTYIAFLTILLTV+ V+PNY+SFGY+FLLLV
Sbjct: 636  RHDNPSYERRIALFLSAIGQKFLSVYRSCGTYIAFLTILLTVYLVRPNYVSFGYIFLLLV 695

Query: 601  WMIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEAS 660
            W+IGRQLVERTK+RLWFPLKAYAI+VF+F+Y LSSF S  +WLS+ IDL FYLG+NSEAS
Sbjct: 696  WIIGRQLVERTKKRLWFPLKAYAIMVFIFMYSLSSFLSIEIWLSKFIDLYFYLGYNSEAS 755

Query: 661  SLQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAAL 720
             L+N W+SLAVLIVMQLYSYERRQS Y+ SD+  +LEF  LGF++RF+IWHS+KILF A+
Sbjct: 756  CLKNIWESLAVLIVMQLYSYERRQSSYNRSDDVHVLEFGVLGFVRRFVIWHSNKILFIAV 815

Query: 721  FYASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMF 780
            FYAS+SPISAFG  YLLGLV+C+TLPK SH PSK FL YTG ++T EYLFQMWGRQA MF
Sbjct: 816  FYASLSPISAFGFFYLLGLVLCSTLPKASHFPSKSFLVYTGFLVTSEYLFQMWGRQAAMF 875

Query: 781  PGQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGK 840
            PGQK SY+S FLGFR F+PGFWGLE GLRGKVL+IAACTLQYNVFRWL++MP + LN GK
Sbjct: 876  PGQKQSYISLFLGFRVFKPGFWGLESGLRGKVLVIAACTLQYNVFRWLKKMPSTILNNGK 935

Query: 841  WDDPCPLFVTEED-DYDISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSA 900
            W++PCPLFV+ ED + + SI  E +K S+DS  +  ++EG+       F  G SQ P+  
Sbjct: 936  WEEPCPLFVSTEDANINGSIPREDNKPSTDSEAISVKREGVRSQSWPFFSPGSSQSPNHV 995

Query: 901  SSKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMEN 960
            S +   SE SSN KYSFG+IWGS KESHKW+K RI++LRKERF+ QK+I KIY+KFWMEN
Sbjct: 996  SPRAGGSEGSSN-KYSFGYIWGSTKESHKWNKKRILALRKERFDTQKLIAKIYLKFWMEN 1055

Query: 961  LFNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILI 1020
            +FNLFGLEI MI+LLLASFALLN++S+ Y+ LLA CI+L+R  IRKLW I VFLFASILI
Sbjct: 1056 MFNLFGLEINMIALLLASFALLNAISMVYIALLATCIILNRQSIRKLWRILVFLFASILI 1115

Query: 1021 LEYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVV 1080
            LEY A W++MW  N   P +    CHDCW+ S  YF +C  CWLGL VDD RML SYF V
Sbjct: 1116 LEYFAIWRSMWTRN--QPDEMIAQCHDCWKSSTMYFSYCKYCWLGLIVDDPRMLISYFAV 1175

Query: 1081 FMLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHL 1140
            FML+  KLRAD LSGFS+SSTYR+M+SQRKN FVWRDLSFETKSMWT  DYLRLYCYCHL
Sbjct: 1176 FMLACFKLRADQLSGFSVSSTYRQMVSQRKNLFVWRDLSFETKSMWTFFDYLRLYCYCHL 1235

Query: 1141 LDLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSL 1200
            LDLVLAL+LITGT+EYD+LHLGYLAFALVFFR+RLEILKK+NK+FKFLR YNFALI+LSL
Sbjct: 1236 LDLVLALVLITGTIEYDILHLGYLAFALVFFRVRLEILKKRNKIFKFLRIYNFALIVLSL 1295

Query: 1201 AYQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSS 1260
            AYQSPFVGE  AGKCET+ Y+FEMIGFYKYDYGFRITARSALVEI+IFMLVS+QSYMFSS
Sbjct: 1296 AYQSPFVGESCAGKCETVDYVFEMIGFYKYDYGFRITARSALVEIVIFMLVSLQSYMFSS 1355

Query: 1261 QEFEYVCRYLEAEQIGAIVREQEKKAAWK---------TEQLQHIRD------------- 1320
             EF+YV RYLEAEQIGAIVREQEKKAAWK         +E+ +H R+             
Sbjct: 1356 PEFDYVSRYLEAEQIGAIVREQEKKAAWKTAQLKHIRESEEKKHQRNLQVEKMKSEMLNL 1415

Query: 1321 -------------------SEERKRQRNLQVEKMK----SEMLNLQIQ----LHNMNSFV 1380
                               SE  +R+RN+ +        S+   LQ++    L   + + 
Sbjct: 1416 QTQLHSTNSVTDCGDSPPVSEGLRRRRNISLNLNNDWGTSDKEGLQLKKEQILREDSLYP 1475

Query: 1381 DGNNVSPSPGN--------------ESFRKRSTSGIHDDAMTTDIEGTPGKKKKHKGSAK 1440
               + SP+P N              ES     T  + DD +   +  T  +K K KG  K
Sbjct: 1476 CELHESPAPVNMENPTGMECARDSIESLNSEITE-VEDD-VADGVFFTSSEKDKVKGKGK 1535

Query: 1441 GNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDDNEQSKTEDRVYDQIESQET 1500
             +PL+S    +GDGVSQVQSIGNQAV+NL SFLN+  + D NE S  ED VYD++ESQ+ 
Sbjct: 1536 ESPLIS---LLGDGVSQVQSIGNQAVNNLVSFLNIDHEFDVNEHSSVEDGVYDEMESQKV 1595

Query: 1501 RYAHLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNF 1560
            + +       +RSSSLQSD SSD  S+QLGRI RHIW+QMR+NND+VCYCCFILVFLWNF
Sbjct: 1596 KVS------FDRSSSLQSDTSSDATSLQLGRILRHIWSQMRSNNDIVCYCCFILVFLWNF 1655

Query: 1561 SLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLL 1620
            SLLSM YLAALF+YALCVN+ P Y+FWVVMLIYTE+YILLQYLYQIIIQH   ++ SDLL
Sbjct: 1656 SLLSMVYLAALFLYALCVNSRPSYIFWVVMLIYTEIYILLQYLYQIIIQHWAFSVTSDLL 1715

Query: 1621 QELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSSAFN---KNALPSKQS 1680
            +E GFP H+ITSSFVVSSLPLFLVY+FTLLQSSITAKDGEW  S+ F+   ++AL  K  
Sbjct: 1716 REWGFPEHKITSSFVVSSLPLFLVYVFTLLQSSITAKDGEWMSSTDFDFYRRSALHGKDI 1775

Query: 1681 LGHYGLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGI 1740
               Y  + +  + L I    +  ++RS  +YW SLT GA+SPPYF+QVS+DV+ WPEDGI
Sbjct: 1776 PISYSWSQKTKKFLQIMENAIKSIIRSFFRYWNSLTHGADSPPYFVQVSMDVRSWPEDGI 1835

Query: 1741 QPERIESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYAS 1800
            QPE+IESG+N +L+IIH ERCKE+NP LC F+SRVHVQSIERS+EN  +AL V EVVYAS
Sbjct: 1836 QPEKIESGVNQLLKIIHDERCKEKNPNLCPFASRVHVQSIERSQENENVALAVFEVVYAS 1895

Query: 1801 PSTNT--CAEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYV 1860
            P T      EW  SLTPAADVA EIL AQ   FVE  GFPY ILSVIGGGKR+ID+YAYV
Sbjct: 1896 PMTECGDSIEWYKSLTPAADVAKEILKAQDAGFVEEIGFPYPILSVIGGGKRDIDVYAYV 1955

Query: 1861 FGADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCS 1920
            FGAD+ VFFLVAIFYQS+ KNNSEFLDVYQLEDQFPKEFVF+LMIIFFLIVLDR IYLCS
Sbjct: 1956 FGADLTVFFLVAIFYQSVRKNNSEFLDVYQLEDQFPKEFVFILMIIFFLIVLDRIIYLCS 2015

Query: 1921 FAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYG 1980
            FA GKVIFYLFNL+LFTY+VTEYAW MEPS+ HAG LALRAIFLAKAVSLALQAIQ+R+G
Sbjct: 2016 FASGKVIFYLFNLILFTYSVTEYAWNMEPSH-HAGRLALRAIFLAKAVSLALQAIQLRHG 2075

Query: 1981 LPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDI 2040
            +PHK TLYRQFLTS++SRINYLGYRLYRALPFLYELRC LDWSCTTTSLTMYDWLKLEDI
Sbjct: 2076 IPHKGTLYRQFLTSEISRINYLGYRLYRALPFLYELRCALDWSCTTTSLTMYDWLKLEDI 2135

Query: 2041 NASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVA 2100
            +ASLYLVKCDAVLNR+ HKQG+KQT MTKCCNGICLFFILICVIWAPMLMYSSGNPTN+ 
Sbjct: 2136 HASLYLVKCDAVLNRATHKQGEKQTKMTKCCNGICLFFILICVIWAPMLMYSSGNPTNIE 2195

Query: 2101 NPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICC 2160
            NPIKDAS QVDIKT SGRLTLYQTTLCEK+ WDKL++++ LDP GYL  YN+ D+QLICC
Sbjct: 2196 NPIKDASVQVDIKTASGRLTLYQTTLCEKLQWDKLDSDVNLDPEGYLDTYNKKDVQLICC 2255

Query: 2161 QADASVLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPN 2220
            ++DAS LWL+PDVVQ+RF+ SLD   ++ ISFTW+L+RDRPKGKE VKY+R +ES+DLP 
Sbjct: 2256 ESDASTLWLIPDVVQTRFIRSLDWDPNMDISFTWVLSRDRPKGKETVKYERSLESQDLPK 2315

Query: 2221 QSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFH 2280
            QSDVQKVLNGS+N FR+ N+Y RYFR  GSG+VRPLE E+ FV+ADL+LNR NYEWWSF 
Sbjct: 2316 QSDVQKVLNGSINSFRMYNIYPRYFR-AGSGDVRPLELEDXFVNADLVLNRANYEWWSFR 2375

Query: 2281 DIQPINVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCS 2331
            D    +++ CG  TGP+A ++SEE PPQGILGDTLSKFSIWGLY+TFVLAVGRFIRLQCS
Sbjct: 2376 DTNSSDINGCGGLTGPMAIIVSEETPPQGILGDTLSKFSIWGLYLTFVLAVGRFIRLQCS 2435

BLAST of Cucsa.084850 vs. NCBI nr
Match: gi|764534725|ref|XP_011458631.1| (PREDICTED: piezo-type mechanosensitive ion channel homolog isoform X1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 3378.6 bits (8759), Expect = 0.0e+00
Identity = 1704/2396 (71.12%), Postives = 1960/2396 (81.80%), Query Frame = 1

Query: 4    QSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLG-SHLRVAS 63
            +SWKSPSV+YFLV+QL  + V+  D++  R GL    DSCW  F S V+ L  SHLRVAS
Sbjct: 99   ESWKSPSVVYFLVLQLSIVAVSLVDLYGNRFGLSSSYDSCWGQFSSTVDRLVCSHLRVAS 158

Query: 64   CLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFL 123
            CLLLPAIQ++VGIS PSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRP  LYAGF+I L
Sbjct: 159  CLLLPAIQLVVGISHPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPFHLYAGFNIVL 218

Query: 124  VYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEMDF 183
            +Y+YQLPVE+P ML+WVA+FIGLFKI+S S+W E+CS+ SL+LFYIML+CVKCDLEEMDF
Sbjct: 219  LYLYQLPVEFPVMLQWVADFIGLFKITSGSQWTEVCSSFSLVLFYIMLACVKCDLEEMDF 278

Query: 184  IMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVAL 243
            I+SM+E+NL+E LLPSKHSFFIR+ RSG +HTNVLL   VFRTF+INFFTYGFPVSLVAL
Sbjct: 279  ILSMKENNLMEHLLPSKHSFFIRQSRSGARHTNVLLTGAVFRTFSINFFTYGFPVSLVAL 338

Query: 244  SFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFL 303
            SFWSFHFAS+CAF LLAYVGY+IYAFPSLF+LHRLNGLLLVFIL WA+STYIFNVAFTFL
Sbjct: 339  SFWSFHFASVCAFALLAYVGYVIYAFPSLFRLHRLNGLLLVFILLWAVSTYIFNVAFTFL 398

Query: 304  NRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSSND 363
            N KIGK+MD+WE+VGLWHYPIPGFFLLAQF LGILVAL NLVNNSVFLCLS E+  SSN+
Sbjct: 399  NWKIGKNMDIWELVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDEEGRSSNE 458

Query: 364  NSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHD 423
            NS     GETKVLIVATIAWGLRK SR I+L LIFL+AMKPGFIHAVYV+FFL+YLLSH+
Sbjct: 459  NSIIEGEGETKVLIVATIAWGLRKCSRPIMLVLIFLIAMKPGFIHAVYVMFFLIYLLSHN 518

Query: 424  VSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFLEI 483
            ++RK+RQSLILL  VHFALLY++QI+ IS+ L++EGSL  E+LLQLGLL ++S W+FLEI
Sbjct: 519  INRKLRQSLILLCEVHFALLYIIQINPISNSLEQEGSLSAEVLLQLGLLEKESSWDFLEI 578

Query: 484  ALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYCHD 543
            ALLACFC IHNHGFEMLFSFSAIV+HTPS P GFSIL+AGLNKSVLLSVY ++++NY HD
Sbjct: 579  ALLACFCAIHNHGFEMLFSFSAIVQHTPSLPFGFSILKAGLNKSVLLSVYASSSTNYSHD 638

Query: 544  NPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMI 603
            NPS+ER+IA FLS+IG+KFLSMYRSCGTYIAFLTIL++V+ ++PNYISFGY+FLLLVW+I
Sbjct: 639  NPSYERRIALFLSAIGQKFLSMYRSCGTYIAFLTILVSVYLMRPNYISFGYIFLLLVWII 698

Query: 604  GRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASSLQ 663
            GRQLVERTK+RLWFPLKAY+I+VF+FIY LSSF S   WLSR IDL FYLG+ S+AS L+
Sbjct: 699  GRQLVERTKKRLWFPLKAYSIMVFIFIYSLSSFPSIEAWLSRLIDLKFYLGYESKASCLE 758

Query: 664  NCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALFYA 723
            N W+SLAVLIVMQLYSYERRQSRY+ SD P+L EF  LGF+KRFLIWHS+KILF A FYA
Sbjct: 759  NVWESLAVLIVMQLYSYERRQSRYNRSDGPDLTEFGVLGFVKRFLIWHSNKILFIAFFYA 818

Query: 724  SISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFPGQ 783
            S+SPISA   LYLLGLVIC+TLPK S IPSK FLAYTG ++T EYLFQM G QA MFPGQ
Sbjct: 819  SLSPISASSFLYLLGLVICSTLPKASRIPSKSFLAYTGFLVTAEYLFQMLGSQAAMFPGQ 878

Query: 784  KHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKWDD 843
            KH  +S  LGFREF+PGFWGLE GLRGKVL+IAACTLQYNVF WLERMP + L+KG   +
Sbjct: 879  KHYNISLLLGFREFKPGFWGLESGLRGKVLVIAACTLQYNVFHWLERMPSTILSKGM-GE 938

Query: 844  PCPLFVTEED-DYDISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSASSK 903
            PCPLF++ ED +   +I +E ++ S+       +QEG        F        + +S K
Sbjct: 939  PCPLFLSAEDTNISATIPSEDNRPSTSFS---VKQEGARSHSWPFFSPSLLHSHNPSSPK 998

Query: 904  RDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENLFN 963
               S+ SS+ KYSFG+IWGS KESHKW+K RI++L+KERFE QK+I KIY+KFW+EN+FN
Sbjct: 999  AGTSKGSSSGKYSFGYIWGSTKESHKWNKKRILALQKERFETQKLISKIYIKFWLENMFN 1058

Query: 964  LFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEY 1023
            LFGLEI MI+LLLASFALLN++S+ Y+ LLAACI+L+R IIRKLWP FVFLFASILILEY
Sbjct: 1059 LFGLEINMIALLLASFALLNAISMLYIALLAACIILNRQIIRKLWPTFVFLFASILILEY 1118

Query: 1024 IAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVFML 1083
             A WK+ W  N P        CHDCW  S  YF +C+ CWLGL VDD RML SYF+VFML
Sbjct: 1119 FAIWKSTWPPNHP--DATNPCCHDCWNNSTMYFSYCMYCWLGLNVDDPRMLISYFIVFML 1178

Query: 1084 SSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDL 1143
            +  KLRADHLS FS SSTYR+M+SQ KNTFVWRDLSFETKSMWT LDY+RLYCYCHLLDL
Sbjct: 1179 ACFKLRADHLSSFSGSSTYREMISQCKNTFVWRDLSFETKSMWTFLDYVRLYCYCHLLDL 1238

Query: 1144 VLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQ 1203
            VLAL+LITGT+EYD+LHLGYLAFALVFFR+RLEILKK+NK+FK+LR YNFALI+LSLAYQ
Sbjct: 1239 VLALVLITGTIEYDILHLGYLAFALVFFRVRLEILKKRNKMFKYLRIYNFALIVLSLAYQ 1298

Query: 1204 SPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQEF 1263
            SPFVG   +GKCE + Y+FEMIGFYKYDYGF+ITARSALVEIIIFMLVS+QSYMFSS+EF
Sbjct: 1299 SPFVG--CSGKCENVDYMFEMIGFYKYDYGFKITARSALVEIIIFMLVSLQSYMFSSKEF 1358

Query: 1264 EYVCRYLEAEQIGAIVREQEKKAAWKTEQLQHIR-----------DSEERKRQR-NLQVE 1323
            ++V RYLEAEQIG IVREQEKKAA KT QLQHIR             E+ K +  NLQ++
Sbjct: 1359 DHVSRYLEAEQIGMIVREQEKKAARKTAQLQHIRESEEKKHQRNLQVEKMKSEMLNLQIQ 1418

Query: 1324 --------------------KMKSEMLNLQIQLHNMNSFVDGNNVSPSPGNESFRKRSTS 1383
                                + +S  LNL     N ++        P    +  R  S  
Sbjct: 1419 LHSMNSVTNCGDSPVSEGLRRRRSTSLNL-----NNDAGTPDKEGFPMKKEQIIRDTSNI 1478

Query: 1384 GIHDDAMTTDIE---------------------------GTP---GKKKKHKGSAKGNPL 1443
             +HD   T ++E                           GT     KK+K KG +K NPL
Sbjct: 1479 ELHDSPATGNLENLVVDSMRNSMQSSHCEITEIEEDVADGTAFDSEKKEKDKGKSKDNPL 1538

Query: 1444 MSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDDNEQSKTEDRVYDQIESQETRYAH 1503
            +SAV  IGDGVSQVQSIGNQAV+NL SFLN+  + D +E S  ED VYD++ESQ+T+Y  
Sbjct: 1539 ISAVHLIGDGVSQVQSIGNQAVNNLVSFLNIDQESDIHEHSP-EDGVYDEMESQKTKY-- 1598

Query: 1504 LERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNFSLLS 1563
               S   RSSSLQSD SSD  S+QLGRIFRHIW++MR+NNDVVCYCCF++VFLWNFSLLS
Sbjct: 1599 ---SSFHRSSSLQSDMSSDATSLQLGRIFRHIWSRMRSNNDVVCYCCFVIVFLWNFSLLS 1658

Query: 1564 MFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLLQELG 1623
            M YLAALF+YALCVN+GP Y+FWVVMLIYTE+YILL YLYQIIIQH GL I S+LL ELG
Sbjct: 1659 MVYLAALFLYALCVNSGPSYIFWVVMLIYTEVYILLLYLYQIIIQHYGLGIASELLHELG 1718

Query: 1624 FPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSSAFN---KNALPSKQSLGHY 1683
            FP H++ SSFVV S P+FLVYLFTL+QSSITAKDGEW  S+  N   +NA   K+    Y
Sbjct: 1719 FPGHKLPSSFVVGSFPIFLVYLFTLIQSSITAKDGEWMSSTDVNLYRRNAFHGKEVPVGY 1778

Query: 1684 GLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGIQPER 1743
              TDRA +L +I    +  + RS  +YW SLTQGAESPPYF+QVS+DV  WPEDGIQPER
Sbjct: 1779 SRTDRAKDLQHILENFIKLIFRSFYRYWGSLTQGAESPPYFLQVSMDVCSWPEDGIQPER 1838

Query: 1744 IESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYASPSTN 1803
            IESG+N +L++IH ERCK ++P+ C  +SRVHVQSIERS+EN  +ALVV EVVYASP T+
Sbjct: 1839 IESGVNQLLRLIHDERCKAKDPKQCPLASRVHVQSIERSQENANVALVVFEVVYASPITD 1898

Query: 1804 TC-AEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVFGADM 1863
               AEW  SLTPAADVA EI  A    +VE  GFPY ILSVIGGGK++IDLYAYVFGAD+
Sbjct: 1899 CASAEWYKSLTPAADVAKEIHNALHAGYVEEIGFPYPILSVIGGGKKDIDLYAYVFGADL 1958

Query: 1864 IVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSFAIGK 1923
             VFFLVAIFYQ +IKN S+FLDVYQLEDQFPKEFVF+LMIIFFLIVLDR IYLCSFA GK
Sbjct: 1959 SVFFLVAIFYQYVIKNKSDFLDVYQLEDQFPKEFVFILMIIFFLIVLDRIIYLCSFATGK 2018

Query: 1924 VIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKS 1983
            VI+YLFNL+LFTY+VT+YAW MEPS+ HAGELALRAIFLAK+VSLALQAIQ+R+G+PHKS
Sbjct: 2019 VIYYLFNLILFTYSVTKYAWYMEPSH-HAGELALRAIFLAKSVSLALQAIQLRHGIPHKS 2078

Query: 1984 TLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2043
            TLYRQFLTS++SRINYLGYRLYRALPFLYELRC LDWSCTTTSLTMYDWLKLEDI+ASLY
Sbjct: 2079 TLYRQFLTSEISRINYLGYRLYRALPFLYELRCALDWSCTTTSLTMYDWLKLEDIHASLY 2138

Query: 2044 LVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKD 2103
            LVKCDAVLNR+ HKQG+KQT MTKCCNGICLFFILICVIWAPMLMYSSGNPTN+ANPIKD
Sbjct: 2139 LVKCDAVLNRATHKQGEKQTQMTKCCNGICLFFILICVIWAPMLMYSSGNPTNIANPIKD 2198

Query: 2104 ASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQADAS 2163
            AS QVDIKTT GRLTLYQ+TLCEKI WD +N+N+ LDP GYL PYN+ D+QLICC+ADAS
Sbjct: 2199 ASVQVDIKTTGGRLTLYQSTLCEKIDWDDVNSNVNLDPQGYLEPYNKKDVQLICCEADAS 2258

Query: 2164 VLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQSDVQ 2223
            VLWLVPDVVQ+RF+ SLD + ++ I FTW L+R+RPKGKEVVKY       DLP QSDVQ
Sbjct: 2259 VLWLVPDVVQTRFIRSLDWESNMAIRFTWELSRERPKGKEVVKYYSYPGFEDLPEQSDVQ 2318

Query: 2224 KVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYE-WWSFHDIQP 2283
            KVLNGS N FRI NVY RY RVTGSG+VRPLE  E  V+ADL++NR +Y  WWSF DI  
Sbjct: 2319 KVLNGSTNSFRIHNVYPRYLRVTGSGDVRPLETGEISVTADLVINRASYPWWWSFLDINS 2378

Query: 2284 INVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2331
             +V+ CG   GP+A ++SEE PPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM
Sbjct: 2379 SDVNGCGGLRGPMAIIMSEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2438

BLAST of Cucsa.084850 vs. NCBI nr
Match: gi|571440238|ref|XP_006575092.1| (PREDICTED: piezo-type mechanosensitive ion channel homolog isoform X1 [Glycine max])

HSP 1 Score: 3370.1 bits (8737), Expect = 0.0e+00
Identity = 1682/2396 (70.20%), Postives = 1960/2396 (81.80%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRV 60
            M +Q+WKSP VIYFLV+QLLA+ VA  DI+  R  L    D  W HF S + HLGSHL+V
Sbjct: 96   MIVQTWKSPYVIYFLVIQLLALLVALVDIYGKRDFLKTWQDPSWGHFISIMEHLGSHLQV 155

Query: 61   ASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSI 120
            ASCLLLPAIQ++VGIS PSW SLPFFIGSCVGLVDWSLTSNFLGLFRWWR LQLYAGF+I
Sbjct: 156  ASCLLLPAIQLVVGISHPSWASLPFFIGSCVGLVDWSLTSNFLGLFRWWRLLQLYAGFTI 215

Query: 121  FLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEM 180
            FL+Y+YQLP+E PSM+ W+A+ IGL+KIS+NSEWP+ICS++SL+ +YIMLS +K DLEEM
Sbjct: 216  FLLYIYQLPMELPSMIHWMADLIGLYKISANSEWPKICSSISLMFYYIMLSFIKSDLEEM 275

Query: 181  DFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV 240
             FI+S  + +L EQLLPSKHSFFIRE RSGV+HTNVLLR  VFRTF+INFFTYGFPVSL 
Sbjct: 276  GFIISRTDCSLTEQLLPSKHSFFIRESRSGVRHTNVLLRGAVFRTFSINFFTYGFPVSLF 335

Query: 241  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFT 300
             LSFWSFHFASLCAFGLLAYVGYI+YAFPSLF+LHRLNGLLLVFILFWA+STYIFNVAFT
Sbjct: 336  VLSFWSFHFASLCAFGLLAYVGYIVYAFPSLFRLHRLNGLLLVFILFWAVSTYIFNVAFT 395

Query: 301  FLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSS 360
            FLN K+G+DM +WEMVGLWHYPIPGFFLLAQF LGILVAL NLVNNSVFLCLS E   SS
Sbjct: 396  FLNWKLGRDMKIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDEGGLSS 455

Query: 361  NDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLS 420
            ND SS    GETKVLIVATIAWGLRK SRAI+LTLIF +A+KPGFIHAVY++FFL+YLLS
Sbjct: 456  NDFSSVKVEGETKVLIVATIAWGLRKCSRAIMLTLIFFIAIKPGFIHAVYMIFFLMYLLS 515

Query: 421  HDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFL 480
            HDVSRKMRQ+LILL  +HF+LLY+LQI+LIS+ L+++GSL  E+++QLGL   DS W+FL
Sbjct: 516  HDVSRKMRQALILLCEIHFSLLYVLQINLISTALEKKGSLSMEVVMQLGLRKEDSAWDFL 575

Query: 481  EIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYC 540
            E+ALLACFC IHNHGFEMLFSFSAI++H P PP+GF IL+AGLNKSVLLSVY++++    
Sbjct: 576  EVALLACFCAIHNHGFEMLFSFSAIIQHAPGPPIGFGILKAGLNKSVLLSVYSSSSVRNS 635

Query: 541  HDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVW 600
             ++ S+ER+IAS+LS+IG+KFLS+YRSCGTYIAF+TILLTV+ V+PN ISFGY+FLLL+W
Sbjct: 636  DESLSYERRIASYLSAIGQKFLSIYRSCGTYIAFVTILLTVYMVRPNCISFGYIFLLLLW 695

Query: 601  MIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASS 660
            +IGRQLVERTKR+LW PLK YAI+VF+FIY LSSFSS  MWLS+ IDL FYLG++S+ASS
Sbjct: 696  IIGRQLVERTKRQLWLPLKVYAILVFIFIYSLSSFSSLEMWLSKLIDLYFYLGYDSKASS 755

Query: 661  LQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALF 720
              N W+SLAVLIVMQLYSYERR+++ +  D  + LE   LGFI+RF+IWHS KILF ALF
Sbjct: 756  FDNVWESLAVLIVMQLYSYERRKNKQNRQDHLDQLEPGALGFIRRFIIWHSQKILFIALF 815

Query: 721  YASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFP 780
            YAS++ ISAFG LYL+GL+ C+ LPK S IPSK FLAYTG ++T EY+FQMWG+QA MFP
Sbjct: 816  YASLNSISAFGFLYLVGLIFCSILPKTSSIPSKSFLAYTGFLVTAEYVFQMWGKQAKMFP 875

Query: 781  GQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKW 840
            GQK+S +S FLGF  FQPGFWGLE GLRGKVL+I ACTLQYNVFRWLERMP + L+KG+W
Sbjct: 876  GQKYSDISLFLGFHVFQPGFWGLESGLRGKVLVIVACTLQYNVFRWLERMPNTVLSKGQW 935

Query: 841  DDPCPLFVTEEDDY-DISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSAS 900
            ++PCPLFV  ED + D ++ NE+SKSS +S      +EG+         SG SQ   + S
Sbjct: 936  EEPCPLFVPTEDVFIDDAMCNEESKSSYNSNLPSAIKEGVSGKSLQIITSGLSQALDTPS 995

Query: 901  SKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENL 960
            SK  +S  SS+ KYSFGFIWGS KES KW+K RI++LRKERFE QK + K+Y+KFWMEN 
Sbjct: 996  SKTGDSSDSSSKKYSFGFIWGSSKESQKWNKKRIVALRKERFETQKTVLKVYLKFWMENT 1055

Query: 961  FNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILIL 1020
            FNLFGLEI MISLLL SFALLN++S+ Y+ LLAAC+LL+R II K+WPIFVFLFASILIL
Sbjct: 1056 FNLFGLEINMISLLLVSFALLNAISMMYIALLAACVLLNRHIICKVWPIFVFLFASILIL 1115

Query: 1021 EYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVF 1080
            EY+A WK+M   N    S+  + CHDCW+ S  +F +C  CWLGL VDD RML SYFVVF
Sbjct: 1116 EYLAIWKDMLPLNSHASSE--IRCHDCWKTSTLHFSYCQKCWLGLIVDDPRMLISYFVVF 1175

Query: 1081 MLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLL 1140
            ML+  KLRAD L  FS SSTYR++MSQR+NTFVWRDLSFETKSMWT +DYLRLYCYCHLL
Sbjct: 1176 MLACFKLRADRLPSFSGSSTYRQIMSQRRNTFVWRDLSFETKSMWTFVDYLRLYCYCHLL 1235

Query: 1141 DLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLA 1200
            DLVL LILITGTLEYD+LHLGYLAFAL+FFR+RLEILKKKNK+FKFLR YNFA+II+SLA
Sbjct: 1236 DLVLILILITGTLEYDILHLGYLAFALIFFRMRLEILKKKNKIFKFLRIYNFAVIIISLA 1295

Query: 1201 YQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQ 1260
            YQSPF+G  SAGKCET + I+EMIGFYKYDYGFRITARSA+VEIIIF+LVS+QSYMFSSQ
Sbjct: 1296 YQSPFIGGPSAGKCETANNIYEMIGFYKYDYGFRITARSAIVEIIIFVLVSLQSYMFSSQ 1355

Query: 1261 EFEYVCRYLEAEQIGAI-----------------VREQEKKAAWKTEQLQHIR------- 1320
            EF+YVCRYLEAEQIGAI                 +RE E+K   +  Q++ ++       
Sbjct: 1356 EFDYVCRYLEAEQIGAIVREQEKKAAWKTAQLQQIRESEEKKQQRNMQVEKMKSEMLNLQ 1415

Query: 1321 ------------------DSEERKRQRNLQ--------VEKMKSEMLNLQIQLHNMNSFV 1380
                              ++E  +R+R++         +   + ++L         +S  
Sbjct: 1416 TQLHSMNTSTNCIDGFSHNNEGLRRRRSVSLASNNDIGIPDKEDQVLGRLDHTIREDSVY 1475

Query: 1381 DGNNVSPS-------PGNESFRKRSTSGIHDDAMTTDIEGTP---GKKKKHKGSAKGNPL 1440
              N   PS       P  E + K S      +    DI+ T    GK++K KG AK NPL
Sbjct: 1476 PINLHEPSVCTNVETPSTEEYMKHSVDSDFCEITEVDIDTTSSDSGKREKFKGQAKENPL 1535

Query: 1441 MSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDD-NEQSKTEDRVYDQIESQETRYA 1500
             SAVQ IGDGVSQVQ IGNQAV+NL SFLN+ P+D D NE S  ED +YD++ESQ+T++ 
Sbjct: 1536 KSAVQLIGDGVSQVQFIGNQAVNNLVSFLNISPEDSDSNEHSNIEDSIYDEMESQKTQHI 1595

Query: 1501 HLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNFSLL 1560
            +++RS     SS+QSDKSSD A +QLGRIFR+IW QM +NNDVVCYCCF+LVFLWNFSLL
Sbjct: 1596 YMDRS-----SSVQSDKSSDAARLQLGRIFRYIWHQMCSNNDVVCYCCFVLVFLWNFSLL 1655

Query: 1561 SMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLLQEL 1620
            SM YL ALF+YALCVNTGP Y+FW++MLIYTELYILLQYLYQI+IQHCGL+I+  LL+EL
Sbjct: 1656 SMMYLGALFLYALCVNTGPSYIFWIIMLIYTELYILLQYLYQIVIQHCGLSIDPHLLREL 1715

Query: 1621 GFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSS--AFNKNALPSKQSLGHY 1680
            GFPTH+ITSSFVVSSLPLFLVYLFTL+Q SIT KDGEW  S+   F +  L +K     Y
Sbjct: 1716 GFPTHKITSSFVVSSLPLFLVYLFTLIQISITPKDGEWMSSTDFKFKRTDLHAKDDRTSY 1775

Query: 1681 GLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGIQPER 1740
               DRA++LL     M+  ++ S  +YWKSLTQGAESPPYF+QVS+DV  WPEDGIQPER
Sbjct: 1776 NWQDRAWDLLNQVINMVKLIIISFFRYWKSLTQGAESPPYFVQVSMDVNFWPEDGIQPER 1835

Query: 1741 IESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYASPSTN 1800
            IESGIN +L+I+H ++CK +NP LCSF+SRV+VQSIERS+E   +ALVV EVVYASP  +
Sbjct: 1836 IESGINQVLRIVHNDKCKAKNPNLCSFASRVNVQSIERSQEKPNVALVVFEVVYASPVID 1895

Query: 1801 -TCAEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVFGADM 1860
             +  EW  SLTPA+DVA EIL AQR  FVE  GFPYRILSVIGGGKREIDLYAY+F AD+
Sbjct: 1896 CSSTEWNKSLTPASDVAKEILKAQRAGFVEEMGFPYRILSVIGGGKREIDLYAYIFCADL 1955

Query: 1861 IVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSFAIGK 1920
            IVFFLVAIFYQS+IKN SEFL+VYQLEDQFPKE+VF+LM IFFLIVLDR IYLCSFA GK
Sbjct: 1956 IVFFLVAIFYQSVIKNKSEFLEVYQLEDQFPKEYVFMLMAIFFLIVLDRIIYLCSFATGK 2015

Query: 1921 VIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKS 1980
            V+FY+FNL+LFTY+VTEY WQ++PS Q   + ALRAIFLAKAVSL LQAIQI+YG+PHKS
Sbjct: 2016 VVFYIFNLILFTYSVTEYDWQLKPS-QRIAQFALRAIFLAKAVSLGLQAIQIQYGIPHKS 2075

Query: 1981 TLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2040
            TLYRQFLTS+VSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY
Sbjct: 2076 TLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2135

Query: 2041 LVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKD 2100
            LVKCD+VLNR  HKQG+KQT MTKCCNGICLFF+LICVIWAPMLMYSSGNPTN+ANPIKD
Sbjct: 2136 LVKCDSVLNRVTHKQGEKQTKMTKCCNGICLFFVLICVIWAPMLMYSSGNPTNIANPIKD 2195

Query: 2101 ASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQADAS 2160
            AS QVDIKT SGRL LYQTTLCE++ WD LN+N   DP GYL  YN++DIQLICCQADAS
Sbjct: 2196 ASFQVDIKTVSGRLNLYQTTLCERLRWDLLNSNANPDPYGYLDAYNKNDIQLICCQADAS 2255

Query: 2161 VLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQSDVQ 2220
             LWLVP VV++R +HSL+   D+ I FTWI +RDRPKGKEVVKY++ ++ + LP QSDVQ
Sbjct: 2256 TLWLVPLVVRTRLIHSLEWNTDMEIFFTWIFSRDRPKGKEVVKYEKAVDPQYLPTQSDVQ 2315

Query: 2221 KVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFHDIQPI 2280
            KVLNGSMN FRI NVY RYFRVTGSG+VRPLE E++ +SADLILNR  +EWW+F D  P 
Sbjct: 2316 KVLNGSMNSFRIYNVYPRYFRVTGSGDVRPLE-EDNALSADLILNREQFEWWAFRDFNPS 2375

Query: 2281 NVSE-CGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2331
            N+S  CG  TGP+A +ISEE PPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQC+DLRM
Sbjct: 2376 NLSGLCGGLTGPMAIIISEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCADLRM 2435

BLAST of Cucsa.084850 vs. NCBI nr
Match: gi|571481307|ref|XP_006588615.1| (PREDICTED: piezo-type mechanosensitive ion channel homolog [Glycine max])

HSP 1 Score: 3368.9 bits (8734), Expect = 0.0e+00
Identity = 1681/2396 (70.16%), Postives = 1958/2396 (81.72%), Query Frame = 1

Query: 1    MTIQSWKSPSVIYFLVVQLLAIFVATADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRV 60
            M +QSWKSP VIYFLV+QLLA+ VA  DI+     L    D  W HF S + HLGSHLRV
Sbjct: 96   MIVQSWKSPYVIYFLVIQLLALLVALVDIYGKSHFLKTWQDPSWGHFLSLIEHLGSHLRV 155

Query: 61   ASCLLLPAIQIIVGISRPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSI 120
            ASCLLLPAIQ++VGIS PSW SLPFFIGSCVGLVDWSLTSNFLGLFRWWR LQLYAGF+I
Sbjct: 156  ASCLLLPAIQLVVGISHPSWASLPFFIGSCVGLVDWSLTSNFLGLFRWWRLLQLYAGFNI 215

Query: 121  FLVYVYQLPVEYPSMLKWVAEFIGLFKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEM 180
            FL+Y+YQLP+E PSM++W+A+ IGL+KIS+NSEWP+ICS++SL+ +YIMLS +K DLEEM
Sbjct: 216  FLLYIYQLPMELPSMIRWMADLIGLYKISANSEWPKICSSISLMFYYIMLSFIKSDLEEM 275

Query: 181  DFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV 240
             FI+S  + +L EQLLPSKHSFFIRE RSGV+HTNVLLR  VFRTF INFFTYGFPVSL 
Sbjct: 276  SFIISRTDCSLTEQLLPSKHSFFIRESRSGVRHTNVLLRGAVFRTFGINFFTYGFPVSLF 335

Query: 241  ALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFT 300
            ALSFWSFHFASLCAFGLLAYVGYI YAFPSLF++HRLNGLLLVFIL WA+STYIFNVAFT
Sbjct: 336  ALSFWSFHFASLCAFGLLAYVGYIAYAFPSLFRMHRLNGLLLVFILLWAVSTYIFNVAFT 395

Query: 301  FLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSS 360
            FLN K+G+DM +WEMVGLWHYPIPGFFLLAQF LGILVAL NLVNNSVFLCLS E   SS
Sbjct: 396  FLNWKLGRDMKIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDEGGQSS 455

Query: 361  NDNSSPGEAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLS 420
            ND+SS    GETKVLIVAT+AWGLRK SRAI+LTLIF +A+KPGFIHAVY++FFL+YLLS
Sbjct: 456  NDHSSVKVEGETKVLIVATVAWGLRKCSRAIMLTLIFFIAIKPGFIHAVYMIFFLVYLLS 515

Query: 421  HDVSRKMRQSLILLNVVHFALLYLLQISLISSVLDREGSLCREILLQLGLLGRDSMWEFL 480
            H+VSRKMRQ+LILL  +HF+LLY+LQI+LIS+ L+++GSL  EI++QLGL   DS W+FL
Sbjct: 516  HNVSRKMRQALILLCEIHFSLLYVLQINLISTALEKKGSLSMEIVMQLGLRNEDSAWDFL 575

Query: 481  EIALLACFCTIHNHGFEMLFSFSAIVEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYC 540
            E+ALLACFC IHNHGFEMLFSFSAI++H PSPP+GF IL+AGLNKSVLLSVY++++    
Sbjct: 576  EVALLACFCAIHNHGFEMLFSFSAIIQHAPSPPIGFGILKAGLNKSVLLSVYSSSSVRNS 635

Query: 541  HDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVW 600
             ++ S+ER+IAS+LS+IG+KFLS+YRSCG+++AF+TILLTV+ V+PNYISFGY+FLLL+W
Sbjct: 636  DESLSYERRIASYLSAIGQKFLSIYRSCGSHVAFVTILLTVYMVRPNYISFGYIFLLLLW 695

Query: 601  MIGRQLVERTKRRLWFPLKAYAIIVFVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASS 660
            +IGRQLVERTKR+LW PLK YAI+VF+FIY LSSFSS  MWLS+ IDL  YLG++S+ASS
Sbjct: 696  IIGRQLVERTKRQLWLPLKVYAILVFIFIYSLSSFSSLEMWLSKLIDLYLYLGYDSKASS 755

Query: 661  LQNCWQSLAVLIVMQLYSYERRQSRYSSSDEPELLEFERLGFIKRFLIWHSDKILFAALF 720
              N W+SLAVLIVMQLYSYERR+++ +  D  + LE   LGFI+RF+IWHS KILF ALF
Sbjct: 756  FDNVWESLAVLIVMQLYSYERRKNKQNRQDHLDQLEPGALGFIRRFIIWHSQKILFIALF 815

Query: 721  YASISPISAFGLLYLLGLVICATLPKISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFP 780
            YAS++ ISAFG LYL+GL+ C+ LPK S IPSK FLAYTG ++T EYLFQMWG+QA MFP
Sbjct: 816  YASLNSISAFGFLYLIGLIFCSILPKTSSIPSKSFLAYTGFLVTAEYLFQMWGKQAKMFP 875

Query: 781  GQKHSYLSYFLGFREFQPGFWGLELGLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKW 840
            GQK+S +S FLGF  FQPGFWGLE GLRGKVL+I ACTLQYNVF WLERMP + L+KG+W
Sbjct: 876  GQKYSDISLFLGFHVFQPGFWGLESGLRGKVLVIVACTLQYNVFHWLERMPNTVLSKGQW 935

Query: 841  DDPCPLFVTEEDDY-DISISNEKSKSSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSAS 900
            ++PCPLFV  ED + D +  NE+SKSS +S      +EG+         SG SQ P + S
Sbjct: 936  EEPCPLFVPTEDAFIDDAKCNEESKSSYNSQLPSAIKEGVSGNSLQIITSGLSQAPDTPS 995

Query: 901  SKRDNSECSSNSKYSFGFIWGSIKESHKWDKIRIISLRKERFELQKIIFKIYMKFWMENL 960
            SK + S  SS+ KYSFGFIWGS KESHKW+K RI++LRKERFE QK + K+Y+KFWMEN 
Sbjct: 996  SKTEGSSDSSSKKYSFGFIWGSSKESHKWNKKRIVALRKERFETQKTVLKVYLKFWMENT 1055

Query: 961  FNLFGLEITMISLLLASFALLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILIL 1020
            FNLFGLEI MISLLL SFALLN++S+ Y+ LLAAC+LL+R IIRK+WPIFVFLFASILIL
Sbjct: 1056 FNLFGLEINMISLLLVSFALLNALSMLYIALLAACVLLNRHIIRKVWPIFVFLFASILIL 1115

Query: 1021 EYIAFWKNMWNSNWPMPSKAGVHCHDCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVF 1080
            EY+A WK+M   N    S+  + C DCW+ S  +F +C  CWLGL VDD RML SYFVVF
Sbjct: 1116 EYLAIWKDMLPLNSHASSE--IRCRDCWKTSTLHFSYCKKCWLGLIVDDPRMLISYFVVF 1175

Query: 1081 MLSSLKLRADHLSGFSLSSTYRKMMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLL 1140
            ML+  KLRAD L  FS SSTYR++MSQR+NTFVWRDLSFETKSMWT +DYLRLYCYCHLL
Sbjct: 1176 MLACFKLRADRLPSFSGSSTYRQIMSQRRNTFVWRDLSFETKSMWTFVDYLRLYCYCHLL 1235

Query: 1141 DLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLA 1200
            DLVL LILITGTLEYD+LHLGYLAFAL+FFR+RLEILKKKNK+FKFLR YNFA+II SLA
Sbjct: 1236 DLVLILILITGTLEYDILHLGYLAFALIFFRMRLEILKKKNKIFKFLRIYNFAVIITSLA 1295

Query: 1201 YQSPFVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQ 1260
            YQSPF+G +SAGKCET++ I+EMIGFYKYDYGFRITARSA+VEIIIF+LVS+QSYMFSSQ
Sbjct: 1296 YQSPFIGGLSAGKCETVNDIYEMIGFYKYDYGFRITARSAIVEIIIFVLVSLQSYMFSSQ 1355

Query: 1261 EFEYVCRYLEAEQIGAI-----------------VREQEKKAAWKTEQLQHIRD------ 1320
            EF+YVCRYLEAEQIGAI                 +RE E+K   +  Q++ ++       
Sbjct: 1356 EFDYVCRYLEAEQIGAIVREQEKKAAWKTAQLQQIRESEEKKQQRNMQVEKMKSEMLNLQ 1415

Query: 1321 ------------------SEERKRQR---------NLQVEKMKSEMLNLQIQLHNMNSFV 1380
                              S E  R+R         ++ +   + ++L         +S  
Sbjct: 1416 IQLLGMNTSTNCIDGFSHSNEGLRRRRSVSLASNNDIGIPDKEDQVLGRLDHTIREDSVY 1475

Query: 1381 DGNNVSPS-------PGNESFRKRSTSGIHDDAMTTDIEGTP---GKKKKHKGSAKGNPL 1440
              N   PS       P  E + K S      +    DI+ +    GKK+K KG AK NPL
Sbjct: 1476 PINLHEPSACTNVESPLTEDYMKHSVDSPFCEITEIDIDTSSSDSGKKEKFKGQAKENPL 1535

Query: 1441 MSAVQFIGDGVSQVQSIGNQAVSNLASFLNVIPDDDD-NEQSKTEDRVYDQIESQETRYA 1500
             SAVQ IGDGVSQVQ IGNQAV+NL SFLN+  +D D NE +  EDR+YD++ESQ+TR+ 
Sbjct: 1536 KSAVQLIGDGVSQVQFIGNQAVNNLVSFLNISQEDSDSNEHTNIEDRIYDEMESQKTRHI 1595

Query: 1501 HLERSHLERSSSLQSDKSSDPASMQLGRIFRHIWAQMRTNNDVVCYCCFILVFLWNFSLL 1560
            +++RS     SS+QSDKSSD AS+QLGRIFR+IW QMR+NNDVVCY CF+LVFLWNFSLL
Sbjct: 1596 YMDRS-----SSVQSDKSSDAASLQLGRIFRYIWHQMRSNNDVVCYFCFVLVFLWNFSLL 1655

Query: 1561 SMFYLAALFVYALCVNTGPGYMFWVVMLIYTELYILLQYLYQIIIQHCGLTINSDLLQEL 1620
            SM YL ALF+YALCVNTGP Y+FW++MLIYTELYILLQYLYQI+IQHCGL+IN  LL+EL
Sbjct: 1656 SMVYLGALFLYALCVNTGPSYIFWIIMLIYTELYILLQYLYQIVIQHCGLSINPHLLREL 1715

Query: 1621 GFPTHRITSSFVVSSLPLFLVYLFTLLQSSITAKDGEWAYSS--AFNKNALPSKQSLGHY 1680
            GFPTH+ITSSFVVSSLPLFLVYLFTL+QSSIT KDGEW  S+   F +N L +K     Y
Sbjct: 1716 GFPTHKITSSFVVSSLPLFLVYLFTLIQSSITPKDGEWMSSTDFKFKRNDLHAKDDHTSY 1775

Query: 1681 GLTDRAYELLYIGRKMMLFVLRSLCKYWKSLTQGAESPPYFIQVSLDVQIWPEDGIQPER 1740
                RA +LL     M+  ++ S  +YWKSLTQGAESPPYF+QVS+DV  WPEDGIQPER
Sbjct: 1776 NWQGRARDLLNQMIIMVKLIIISFFRYWKSLTQGAESPPYFVQVSMDVNFWPEDGIQPER 1835

Query: 1741 IESGINHMLQIIHVERCKEQNPRLCSFSSRVHVQSIERSKENTKIALVVLEVVYASPSTN 1800
            IESGIN +L+I+H ++CK +NP LCSF+SRV+VQSIERS+E   +ALVV EVVYASP  +
Sbjct: 1836 IESGINQVLRIVHNDKCKAKNPNLCSFASRVNVQSIERSQEKPNVALVVFEVVYASPVID 1895

Query: 1801 -TCAEWCDSLTPAADVANEILLAQRNEFVESTGFPYRILSVIGGGKREIDLYAYVFGADM 1860
             +  EW  SLTPA+DVA EIL AQR  FVE  GFPYRILSVIGGGKREIDLYAY+F AD+
Sbjct: 1896 CSSTEWNKSLTPASDVAKEILKAQRAGFVEEMGFPYRILSVIGGGKREIDLYAYIFCADL 1955

Query: 1861 IVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVFVLMIIFFLIVLDRCIYLCSFAIGK 1920
            IVFFLVAIFYQS+IKN SEFL+VYQLEDQFPKE+VF+LM IFFLIVLDR +YLCSFA  K
Sbjct: 1956 IVFFLVAIFYQSVIKNKSEFLEVYQLEDQFPKEYVFMLMAIFFLIVLDRILYLCSFATWK 2015

Query: 1921 VIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKS 1980
            V+FY+FNLVLFTY+VTEY WQ+EPS QH  + ALRAIFLAKAVSL LQAIQI+YG+PHKS
Sbjct: 2016 VVFYIFNLVLFTYSVTEYDWQLEPSQQHTAQFALRAIFLAKAVSLGLQAIQIQYGIPHKS 2075

Query: 1981 TLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2040
            TLYRQFLTS+VSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY
Sbjct: 2076 TLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLY 2135

Query: 2041 LVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKD 2100
            LVKCD+VLNR  HKQG+KQT MTKCCNGICLFF+LICVIWAPMLMYSSGNPTN+ANPIKD
Sbjct: 2136 LVKCDSVLNRGTHKQGEKQTKMTKCCNGICLFFVLICVIWAPMLMYSSGNPTNIANPIKD 2195

Query: 2101 ASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVLDPGGYLSPYNQDDIQLICCQADAS 2160
            AS QVDIKT SGRL LYQTTLCE++ WD LN+N+  DP GYL  YN++DIQLICCQADAS
Sbjct: 2196 ASFQVDIKTASGRLNLYQTTLCERLQWDLLNSNINPDPYGYLGAYNKNDIQLICCQADAS 2255

Query: 2161 VLWLVPDVVQSRFVHSLDRKQDIIISFTWILTRDRPKGKEVVKYDRVIESRDLPNQSDVQ 2220
             LWLVP VV++R + SL+   D+ I  TWIL+RDRPKGKE+VKY++ ++ + LP +SDVQ
Sbjct: 2256 TLWLVPLVVRTRLIQSLEWNIDMEIFSTWILSRDRPKGKEIVKYEKAVDPQYLPTRSDVQ 2315

Query: 2221 KVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEESFVSADLILNRNNYEWWSFHDIQPI 2280
            KVLNGSMN F I NVY RYFRVTGSG+VRPLE E++ VSADLI+NR   EWW+F D  P 
Sbjct: 2316 KVLNGSMNSFSIYNVYPRYFRVTGSGDVRPLE-EDNAVSADLIINREQLEWWAFRDTNPS 2375

Query: 2281 NVSE-CGRFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRM 2331
            N+S  CG  TGP+A ++SEE PPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQC+DLRM
Sbjct: 2376 NLSRLCGGLTGPMAIIVSEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCADLRM 2435