Cucsa.084820 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.084820 vs. Swiss-Prot
Match: AT18G_ARATH (Autophagy-related protein 18g OS=Arabidopsis thaliana GN=ATG18G PE=2 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.4e-143
Identity = 297/626 (47.44%), Postives = 404/626 (64.54%), Query Frame = 1

Query: 304 WKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNI 363
           WK G ++G +A+  GMVAVKD V+ A+++QF+AHTSP+SALCFDPSGTLLVTAS+ GNNI
Sbjct: 344 WKVGGVSGSDAENAGMVAVKDLVSGALVSQFKAHTSPISALCFDPSGTLLVTASVCGNNI 403

Query: 364 NIFRIIPNSRSGSSG-LSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGT 423
           N+F+I+P+    + G LS++  SSHVHL+KLHRGITSA++QDICFS  SQWVAI+SSKGT
Sbjct: 404 NVFQIMPSRSHNAPGDLSYEWESSHVHLFKLHRGITSAIVQDICFSQQSQWVAIISSKGT 463

Query: 424 CHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVS 483
           CH+F+L+  G +A  +    +GEEP  LP  +LPWW T SL  NQQS  PP  V LSVVS
Sbjct: 464 CHIFVLNSSGSDAAFQ--PCEGEEPTRLPASSLPWWFTQSLSSNQQSLSPPTAVALSVVS 523

Query: 484 RIKYSSFGWLNTVNNS--AGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYT 543
           RIKYSSFGWLNTV+N+  A  GK FVPSGAVAA+FH +++H++Q +NS+ N+LEH+LVYT
Sbjct: 524 RIKYSSFGWLNTVSNATTAATGKVFVPSGAVAAVFHKSVTHDLQ-LNSRTNALEHILVYT 583

Query: 544 PSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERG 603
           PSGHVVQHELLPS   E   +  R + +S + +QEDDL++KVEPIQWWD CRRSD+ E  
Sbjct: 584 PSGHVVQHELLPSVCTESPENGLRVQKTSHVQVQEDDLRVKVEPIQWWDVCRRSDWLETE 643

Query: 604 ECIHDSTSDGQDIAKTNAIQNERTDTEKT-HELDFQEMNDGSSADEILRVRGQSGITHEQ 663
           E +  S ++ Q   +T  + N  T  E     LD   MN   S D+ L+   +     E+
Sbjct: 644 ERLPKSITEKQYDLET--VSNHLTSHEDACLSLD---MNSHFSEDKYLKSCSEK--PPER 703

Query: 664 SHWFLSNAEVQLSSGRLPIWQNSKI--YVMSSPRINSIAGGEFEIEKVPVQEIEVREKEL 723
           SH +LSN EV+++SG LP+WQNSKI  +VM SPR +S  GGEFEIEKVP  E+E+++K+L
Sbjct: 704 SHCYLSNFEVKVTSGMLPVWQNSKISFHVMDSPRDSSSTGGEFEIEKVPAHELEIKQKKL 763

Query: 724 LPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESS 783
           LP FDH  S K+   DR  +  +C   +++  +Q   K+ + I + CHSKP S+ S ESS
Sbjct: 764 LPVFDHFHSTKATLEDRFSM--KCYHTSATGSHQVNGKICQDI-INCHSKPGSIESAESS 823

Query: 784 DGGSSRRMESSIDFDQASCE-KSCTPLCQHLNEMY--WEKRASESFTTPKSSNILCTQVE 843
           + GS+++ME+  D D  S   KS  PL   +N +Y   EK  +  +     +  L T  E
Sbjct: 824 EEGSTKQMENLHDSDHMSNSIKSSLPLYPTVNGIYKEIEKNNANGWMEKPVTAKLSTLKE 883

Query: 844 GSRIDGSPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVLESNDGCQDIN 903
               +G       ++S     + + +S+  PP     F     EE CK +   D  ++  
Sbjct: 884 TRITNGFTTPPILTDS----VNEQMLSTGKPPMG---FGFALHEEHCKAVA--DPKEEHL 943

Query: 904 DISTDHVDGFEYENICSDENDKIFGD 921
               D V    + N+ ++  +K+ GD
Sbjct: 944 KKKLDEVTNVHHLNVNNNNTEKLQGD 947

HSP 2 Score: 352.1 bits (902), Expect = 1.9e-95
Identity = 192/311 (61.74%), Postives = 223/311 (71.70%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKG+N GLLPNS KIISSCLKTVS NA+ VAS+VRSAGASVAASISAA  ED+KDQV
Sbjct: 2   MKKGKGKNSGLLPNSFKIISSCLKTVSANATNVASSVRSAGASVAASISAA--EDDKDQV 61

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGF ILE+  ++ RH+LLLGY NGFQ+ DVEDASNF ELVSKRGGPVSFLQ+ P PA+
Sbjct: 62  TWAGFGILELGQHVTRHVLLLGYQNGFQVFDVEDASNFNELVSKRGGPVSFLQMQPLPAR 121

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLP---------GSCANS 180
            G        HE    SHPLLL+VAG+E+    +G + S  G L          G   N 
Sbjct: 122 SGD-------HEGFWNSHPLLLVVAGDETNGTGLGHSFSQNGSLARDGSSDSKAGDAINY 181

Query: 181 HNAVQFYSLKSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSP 240
              V+FYSL+SHSYV+VLRFRS+VCM+RCSS++VAVGLA QIYC DA+TLE  FSVLT P
Sbjct: 182 PTTVRFYSLRSHSYVYVLRFRSSVCMIRCSSRVVAVGLANQIYCVDALTLENKFSVLTYP 241

Query: 241 VPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGS 300
           VP+   QGTT  NVGYGPMAVGPRWLAY S   +   T  LS Q+   S S++P S  G 
Sbjct: 242 VPQPVRQGTTRVNVGYGPMAVGPRWLAYASKSSMTMKTGRLSPQTFTSSPSLSPSSSSGG 301

Query: 301 DRTKAHYPVKS 303
               A Y ++S
Sbjct: 302 SSFMARYAMES 303

BLAST of Cucsa.084820 vs. Swiss-Prot
Match: AT18H_ARATH (Autophagy-related protein 18h OS=Arabidopsis thaliana GN=ATG18H PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 3.0e-104
Identity = 222/464 (47.84%), Postives = 295/464 (63.58%), Query Frame = 1

Query: 303 GWKAGRLAG--MEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYG 362
           G K GR+     E+D  G V VKDF +RAII QFRAHTSP+SALCFDPSGTLLVTASI+G
Sbjct: 344 GRKVGRVGSHSAESDVVGTVIVKDFESRAIIAQFRAHTSPISALCFDPSGTLLVTASIHG 403

Query: 363 NNINIFRIIPN-SRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSS 422
           NNIN+FRI+P  +++G    S+D +SSHV LYKLHRG+TSA+IQDICFS+YSQW+AIVSS
Sbjct: 404 NNINVFRIMPTPTKNGPGAQSYDWSSSHVPLYKLHRGMTSAVIQDICFSSYSQWIAIVSS 463

Query: 423 KGTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLS 482
           K TCH+++LSPFGGE  L I NSQ + P + P L+LPWWS+ S +    S+PPP  VTLS
Sbjct: 464 KSTCHIYVLSPFGGENVLEIRNSQFDGPTLAPTLSLPWWSSPSFMTTHFSYPPPASVTLS 523

Query: 483 VVSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVY 542
           VVSRIK ++F        S+  GK   PSG +AA+FH ++    Q   S   +L++LLVY
Sbjct: 524 VVSRIKCNNFFHA----ASSVVGKPTFPSGCLAAVFHQSVP---QESQSSSPALDYLLVY 583

Query: 543 TPSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSER 602
           TPSGHVVQ++L+PS G + +  ++R  ++S L   E++L++KVEP+Q WD CRR+D+ ER
Sbjct: 584 TPSGHVVQYKLIPSLGGDQAESNTRNGATSGL-TSEEELRVKVEPVQCWDVCRRADWPER 643

Query: 603 GECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQ 662
            E I   T  G+  A+     ++  D  K                             E+
Sbjct: 644 EENICGLTYGGRKNAELTVDTSDSEDQTK---------------------------PLEK 703

Query: 663 SHWFLSNAEVQLSSGRLPIWQNSKI--YVMSSP-----RINS-IAGGEFEIEKVPVQEIE 722
            H +L+NAEV ++SGR PIWQNS+I  Y M  P      +NS   GGE EI KV   E++
Sbjct: 704 HHVYLANAEVLINSGRKPIWQNSEISFYPMYPPDSDGKNLNSHQGGGETEIGKVSANEVD 763

Query: 723 VREKELLPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVK 756
           +R K+LLP +D+  S+ +   +RG   G   S +SS     +VK
Sbjct: 764 IRRKDLLPVYDNFHSVYTSMRNRG-FSGERDSDSSSSSDPGQVK 771

HSP 2 Score: 259.6 bits (662), Expect = 1.3e-67
Identity = 153/302 (50.66%), Postives = 193/302 (63.91%), Query Frame = 1

Query: 3   KGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQVTW 62
           K  G N G LPNSLK IS+C++T S       S VRSA ASVAAS+S+ S E  KDQV W
Sbjct: 20  KNNGTN-GFLPNSLKFISTCIRTAS-------SGVRSASASVAASLSSDSHE-LKDQVLW 79

Query: 63  AGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAKPG 122
           + FD L       +++LLLGY NGFQ+LD++D+++  E VS+R  PV+FLQ+ P PAK  
Sbjct: 80  SSFDRLHTSESSFKNVLLLGYTNGFQVLDIDDSNDVTEFVSRRDDPVTFLQMQPLPAK-- 139

Query: 123 TPPAKSDRHELLRRSHPLLLIVAGEE--SKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 182
                 D  E  R SHP+LL VA E   S  +   ++ S         A S   V+FYSL
Sbjct: 140 -----CDGVEGFRSSHPILLAVADEAKGSGPIVTSRDGSVRNGYEDPLALSPTVVRFYSL 199

Query: 183 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT 242
           +SH+YVHVLRFRS V MVRCS +IVAVGL +QIYCFDA+TLE  FSVL+ PVP++  QG 
Sbjct: 200 RSHNYVHVLRFRSTVYMVRCSPRIVAVGLGSQIYCFDALTLENKFSVLSYPVPQLGNQGI 259

Query: 243 TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV 302
           +G NVGYGPMAVG RWLAY S  P+ S+   LS Q+  P   V+P + P +    A Y +
Sbjct: 260 SGVNVGYGPMAVGARWLAYASNSPLSSSIGRLSPQNVTP-PGVSPSTSPNNGNLVARYAM 304

BLAST of Cucsa.084820 vs. Swiss-Prot
Match: AT18F_ARATH (Autophagy-related protein 18f OS=Arabidopsis thaliana GN=ATG18F PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 8.4e-83
Identity = 260/823 (31.59%), Postives = 381/823 (46.29%), Query Frame = 1

Query: 14  NSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQVTWAGFDILEVEPY 73
           +S + +S+CLK +S+ ASTVA +  SA +S          E   DQV WAGFD L+ E  
Sbjct: 23  SSFRALSNCLKVISSGASTVARSAVSAASSAV--------ESHHDQVLWAGFDNLQKEDG 82

Query: 74  IIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAKPGTPPAKSDRHEL 133
             R +LLL + +GFQ+ DVED  N   +VS   G   F+Q+L +P   G      DR   
Sbjct: 83  DTRRVLLLAFKSGFQVWDVEDTENVHVIVSAHDGQAFFMQMLLNPINSGV---LDDR--- 142

Query: 134 LRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSLKSHSYVHVLRFRS 193
             +S PLL +     SK ++     S     P +       V  YSLKS SYVH L+FR+
Sbjct: 143 FYKSRPLLAVCGDYSSKKISSDNPGSETVATPTN-------VYVYSLKSQSYVHTLKFRA 202

Query: 194 AVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGTTGTNVGYGPMAVG 253
            +  VRC S+IVAV  A QI CFDA TLE+ + ++T+ +      G+ G  VGYGP+AVG
Sbjct: 203 TIYSVRCCSRIVAVLQAAQIDCFDAATLEMDYRIVTNSIV----CGSLG--VGYGPLAVG 262

Query: 254 PRWLAYP-------SVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSD----------RTKA 313
           PRW+AY        S     S  V LS+ SP  +Q     S   +           R+  
Sbjct: 263 PRWIAYSGSRIATSSSAIFTSEIVSLSTSSPSVAQFARDSSKQLASGIANLGDKGYRSLT 322

Query: 314 HY----------PVKSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCF 373
            Y          P   G   G     +A+  GMV VKD   +++ITQF+AH SP+SALCF
Sbjct: 323 KYCSEVLPNPYIPGLKGIGVGNEKVADAESIGMVIVKDITNKSVITQFKAHKSPISALCF 382

Query: 374 DPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDIC 433
           D SG LLVTASI G+NIN+FRI+P   +  +  +    ++  HL++L RG T+A+IQDIC
Sbjct: 383 DQSGLLLVTASIQGHNINVFRIMPTISTSRAVKT----TTFAHLFRLQRGFTNAVIQDIC 442

Query: 434 FSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIIN 493
           FS  S  + + SS+GT H+F ++P            +G+ P                   
Sbjct: 443 FSKDSNLIVVGSSRGTSHLFEINP----------EKEGDAPV------------------ 502

Query: 494 QQSFPPPPPVTLSVVSRIKY-SSFGWLNTVNNSAGAGKGFVPS---GAVAAIF------- 553
                      +S +SRI+  +S GW+ TV+ +A A  G V     G V + F       
Sbjct: 503 ----------PMSAISRIRSGNSSGWIGTVSGAASAAAGMVAGSVPGTVTSTFCYCDEKS 562

Query: 554 HNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLP---SFGAEPSLHSSRTESSSVLH 613
           +N    ++  + SK N    LLV+ PSG + Q+ L       G E +  +     S +  
Sbjct: 563 NNNYYGSVADMCSKTN----LLVFAPSGCMTQYALREHQVGVGHETAAMTGFDSESGL-- 622

Query: 614 MQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQNERTDTEKTHEL 673
             E + KL V+PI+ W   +     E     HD  SD         I    T  +   ++
Sbjct: 623 --ETEGKLAVDPIRRWSMIQNQSRRE----THDPHSD---------IYGGGTSVDSKSKV 682

Query: 674 DFQEMNDGSSADEILRVRGQSGITH--EQSHWFLSNAEVQLS--SGRLPIWQNSK----- 733
            F E+    S +E  +V  + G T   ++ H ++  AE Q +    +LP+W   K     
Sbjct: 683 -FPEVVRKQSVEEAWKV-SKKGTTRVVDKRHLYIYEAEQQQTHLPTQLPLWARRKFRFQE 742

Query: 734 IYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRCIS 787
           + +     I+   G E EIE +  + IE R ++L+P + +  S +S       ++   I 
Sbjct: 743 LVLNRGEEISGGGGREMEIEGIQTRTIEARTRDLVPVWGYLQSPRS-----QQVINESIQ 743

BLAST of Cucsa.084820 vs. Swiss-Prot
Match: BCAS3_DROME (Breast carcinoma-amplified sequence 3 homolog OS=Drosophila melanogaster GN=rudhira PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 4.5e-28
Identity = 127/463 (27.43%), Postives = 197/463 (42.55%), Query Frame = 1

Query: 54  EDEKDQVTWAGF------------DILEVEPYIIRHILL-LGYLNGFQLLDVEDASNFKE 113
           +D KD +TWA F            D  E+E      +LL LGY  G Q+  +       E
Sbjct: 75  QDPKDTITWARFETCADVSDPRFGDDWELEGNAAPPLLLILGYGLGVQVWAIPANGEAVE 134

Query: 114 LVSKRGGPVSFLQILPSPAKPGTPPAKSDRHELL---RRSHPLLLIVAGEESKDVAMGQN 173
           ++S R G V+ L++LP+PA            E +       PL+  V G  +    +   
Sbjct: 135 VLSWRHGVVTALRVLPTPATAAALDENGRADEPVDSFAEKRPLVAFVDGGSAAASGLLAG 194

Query: 174 HSPMGVLPG-------------------SCANSHNAVQFYSLKSHSYVHVLRFRSAVCMV 233
            S +G+  G                   S +   +AV F SLK+   V  ++F++AV  +
Sbjct: 195 SSGLGLGGGGGGVTTVGGSVGGVSGIGVSASAQFSAVNFMSLKTGVQVKTIKFKNAVLDI 254

Query: 234 RCSSQIVAVGLATQIYCFDAITLEVVFSVLTS-PVPEISGQGTTGTNVGYGPMAVGPRWL 293
           + +   V +    +I  FDA TLE   ++ T  P P I+            P+A+GPRWL
Sbjct: 255 QANRSAVVITFHERIAVFDARTLEDRLTITTCYPSPGINP----------NPIALGPRWL 314

Query: 294 AYP---------SVG-----PVPSTTVPLSSQSPFPSQSV------------NPPSLPGS 353
           AY          S G      VPS T  + + +   S+ +               +  G+
Sbjct: 315 AYAEHKLLHSKRSGGGCDGEGVPSYTATVLNAAKSLSKGLREFGEQVAAGLTGTTAGSGA 374

Query: 354 DRTKAHYPVKSGWKAGRLAGMEADYPGMVAVKDFVTRA----------------IITQFR 413
               + +   SG    + +G+         VKD+   +                I+  F 
Sbjct: 375 SSKSSSFDSASGGPDAKQSGVVTIIDVKHPVKDYSPTSGTPLSSTAGSQGGGDPIVAHFV 434

Query: 414 AHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHR 439
           AH+  L A+ FD SG LL+TA   G++ ++FR+ P+    S        ++  HLY LHR
Sbjct: 435 AHSEALVAMEFDSSGMLLLTADRRGHDFHVFRVQPHPVGPSL-------AAVHHLYVLHR 494

BLAST of Cucsa.084820 vs. Swiss-Prot
Match: BCAS3_MOUSE (Breast carcinoma-amplified sequence 3 homolog OS=Mus musculus GN=Bcas3 PE=1 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 2.3e-16
Identity = 49/122 (40.16%), Postives = 74/122 (60.66%), Query Frame = 1

Query: 318 GMVAV-KDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPNSRSGS 377
           G V V +D  +  I+  F AH  P+  + F+ SG LLVT    G++ ++F+I+ +  S S
Sbjct: 330 GQVLVSEDSDSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQILTHPWSSS 389

Query: 378 SGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAG 437
                 C + H HLY LHRG T A +QDICFS+  +WV + + +GT HVF ++P+GG+  
Sbjct: 390 Q-----C-AVH-HLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPC 444

Query: 438 LR 439
           +R
Sbjct: 450 VR 444

HSP 2 Score: 64.7 bits (156), Expect = 6.1e-09
Identity = 63/246 (25.61%), Postives = 104/246 (42.28%), Query Frame = 1

Query: 28  TNASTVASTVRSAGASVAASISAASSEDEKDQVTWAGF---DILEVEPYIIRH------- 87
           T  S + S V      V  + S +   +EK+++ W  F   D+ +    +  H       
Sbjct: 28  TEQSYMESVVTFLQDVVPQAYSGSPLTEEKEKIVWVRFENADLNDTSRNLEFHELHSTGN 87

Query: 88  ----ILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAKPGTPPAKSDRHEL 147
               ++++GY +G Q+  +  +   +EL S R GPV   +ILP+      P   + + + 
Sbjct: 88  EPPLLVMIGYSDGMQVWGIPISGEAQELFSVRHGPVRAARILPA------PQLGAQKCDN 147

Query: 148 LRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSLKSHSYVHVLRFRS 207
                PLL +     S            G  P  C      V  YSL++   V  ++F++
Sbjct: 148 FAEKRPLLGVCKSIGSS-----------GTTPPYC-----CVDLYSLRTGEMVKSIQFKT 207

Query: 208 AVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGTTGTNVGYGPMAVG 260
            +  + C+ +I+ V L  +I  FD+ T    F V TS  P        G N+   P+A+G
Sbjct: 208 PIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFV-TSCYP------CPGPNM--NPIALG 242

BLAST of Cucsa.084820 vs. TrEMBL
Match: A0A0A0K530_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G391270 PE=4 SV=1)

HSP 1 Score: 1876.7 bits (4860), Expect = 0.0e+00
Identity = 927/928 (99.89%), Postives = 927/928 (99.89%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV
Sbjct: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK
Sbjct: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 180
           PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL
Sbjct: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 180

Query: 181 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT 240
           KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT
Sbjct: 181 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT 240

Query: 241 TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV 300
           TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV
Sbjct: 241 TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV 300

Query: 301 KSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYG 360
           KSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYG
Sbjct: 301 KSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYG 360

Query: 361 NNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSK 420
           NNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSK
Sbjct: 361 NNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSK 420

Query: 421 GTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSV 480
           GTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSV
Sbjct: 421 GTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSV 480

Query: 481 VSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYT 540
           VSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYT
Sbjct: 481 VSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYT 540

Query: 541 PSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERG 600
           PSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERG
Sbjct: 541 PSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERG 600

Query: 601 ECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQS 660
           ECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQS
Sbjct: 601 ECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQS 660

Query: 661 HWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPC 720
           HWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPC
Sbjct: 661 HWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPC 720

Query: 721 FDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESSDGG 780
           FDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTE IVVICHSKPASLSSTESSDGG
Sbjct: 721 FDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTEEIVVICHSKPASLSSTESSDGG 780

Query: 781 SSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDG 840
           SSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDG
Sbjct: 781 SSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDG 840

Query: 841 SPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDH 900
           SPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDH
Sbjct: 841 SPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDH 900

Query: 901 VDGFEYENICSDENDKIFGDMFTSSEEG 929
           VDGFEYENICSDENDKIFGDMFTSSEEG
Sbjct: 901 VDGFEYENICSDENDKIFGDMFTSSEEG 928

BLAST of Cucsa.084820 vs. TrEMBL
Match: M5VIN0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000785mg PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 9.4e-275
Identity = 536/928 (57.76%), Postives = 654/928 (70.47%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKK KG+N GLLPNSL+IISSCLKTVSTNASTVASTVRSAGASVAASISA  SED+KDQV
Sbjct: 1   MKKSKGKNNGLLPNSLRIISSCLKTVSTNASTVASTVRSAGASVAASISA--SEDQKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGF  LE+     +H+LLLGY NGFQ+ DVEDASNF ELVSKR GPVSFLQ+ PSPA 
Sbjct: 61  TWAGFGRLELSHSAFKHVLLLGYQNGFQVFDVEDASNFSELVSKRDGPVSFLQMQPSPAA 120

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVL---------PGSCANS 180
                  SD ++  R +HPLLL+VAG+++    +  N S +G +         PG+   S
Sbjct: 121 -------SDGNQGFRMAHPLLLVVAGDDTNGPGIVHNTSHLGGIGRDSNLESRPGNPVGS 180

Query: 181 HNAVQFYSLKSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSP 240
             AV+FYSL+SH YVHVLRFRSAVCM+RCS +IVAVGLATQIYCFDA+TLE  FSVLT P
Sbjct: 181 PTAVRFYSLRSHGYVHVLRFRSAVCMIRCSPRIVAVGLATQIYCFDALTLENKFSVLTYP 240

Query: 241 VPEISGQGTTGTNVGYGPMAVGPRW-------------------LAYPSVGPVPSTT--- 300
           VP+++GQG+ G NVGYGPMAVGPRW                      PS G  PST+   
Sbjct: 241 VPQLAGQGSIGFNVGYGPMAVGPRWLAYASNSPLVSNTGRLGPQNLTPSPGVSPSTSPGS 300

Query: 301 ------VPLSSQSPFPSQSVNPPSLPGS-----------DRTKAHYPVKSGWKAGRLAGM 360
                   + S     +  +N   +              D + +     SGWK  R AG 
Sbjct: 301 GSYVARYAMESSKHLAAGIINLGDMGCKTLYKYCQDLLPDGSNSPISSNSGWKVSRHAGT 360

Query: 361 EADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPNS 420
           E D  GMV VKDFV++A+I+QF+AHTSP+SALCFDPSGTLLVTASIYGNNINIFRI+P+S
Sbjct: 361 EMDNAGMVVVKDFVSQAVISQFKAHTSPISALCFDPSGTLLVTASIYGNNINIFRIMPSS 420

Query: 421 R-SGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPF 480
           + SGS G + D +SSHVHLYKLHRGITSAMIQDICFS+YSQWVAIVSSKGTCHVF+LSPF
Sbjct: 421 KHSGSGGQNLDWSSSHVHLYKLHRGITSAMIQDICFSHYSQWVAIVSSKGTCHVFVLSPF 480

Query: 481 GGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGW 540
           GG+AG R+LN+QGEEP + PVL+LPWWSTSS I NQQS PPP PV LSVVSRIKYSSFGW
Sbjct: 481 GGDAGFRLLNTQGEEPSLYPVLSLPWWSTSSCIFNQQSCPPPAPVALSVVSRIKYSSFGW 540

Query: 541 LNTVNNSAGA--GKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHE 600
           L+ VNN+A +  GK FVPSGAVAA+FHN+LS + +  NS+ ++LEHLLVYTPSGHVVQHE
Sbjct: 541 LSPVNNTASSTTGKVFVPSGAVAAVFHNSLSQSPRQSNSRTSTLEHLLVYTPSGHVVQHE 600

Query: 601 LLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSD 660
           L P  G + S   S T++++ +HMQE+DL++KVEPIQWWD CRRSD+ ER + +  +TSD
Sbjct: 601 LQPRIGVDQS--HSGTQAATSMHMQEEDLRVKVEPIQWWDVCRRSDWPEREDIVLGTTSD 660

Query: 661 GQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEV 720
            QD+A+ N     ++ ++ TH ++  ++N     +   R+   SG  +++SHW+LSNAEV
Sbjct: 661 RQDVAEIN---QTKSGSDGTHGMESLDLNGAVGGER--RLETYSGKLNDRSHWYLSNAEV 720

Query: 721 QLSSGRLPIWQNSKI--YVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSL 780
           Q+SS RLPIWQ SKI  Y M  PR++S A GEFEIEKVPV EIE+R+KELLP F+    +
Sbjct: 721 QISSLRLPIWQKSKICFYTMGCPRVDSFADGEFEIEKVPVHEIEMRQKELLPVFEQFHII 780

Query: 781 KSGCNDRGLLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESSDGGSSRRMES 840
           KS  +DR +  GR  S +SSE +Q++ K+ E   VICHSKPASLSSTESSDGGSSRR+E 
Sbjct: 781 KSSWDDR-VPGGRFPSHSSSEPHQAQDKILEE-TVICHSKPASLSSTESSDGGSSRRIEH 840

Query: 841 SIDFDQASCEKSCTPLCQHLNEMYWEKRAS----ESFTTPKSSNILCTQVEGSRIDGSPC 872
            +DFDQ + EK+ T +CQ LN    E+RA+     S     S +ILCT  E  +   S  
Sbjct: 841 FLDFDQTNNEKARTTVCQILNGP--ERRANTIVEPSLENHISFSILCTPSEHFKNIDSQV 900

BLAST of Cucsa.084820 vs. TrEMBL
Match: A0A067HHB0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g001814mg PE=4 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 5.7e-272
Identity = 551/1013 (54.39%), Postives = 672/1013 (66.34%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKGRN GLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASIS AS ED KDQV
Sbjct: 1   MKKGKGRNNGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISNAS-EDLKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGFD LE  P + + +LLLGY NGFQ+LDVEDASNF ELVSKR GPVSFLQ+ P P K
Sbjct: 61  TWAGFDRLEYGPSVFKQVLLLGYQNGFQVLDVEDASNFNELVSKRDGPVSFLQMQPFPVK 120

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVL--------PGSCANSH 180
                   D  E  R+ HP LL+VAGE++  +A GQN S +G +         G+C NS 
Sbjct: 121 -------DDGCEGFRKLHPFLLVVAGEDTNTLAPGQNRSHLGGVRDGMMDSQSGNCVNSP 180

Query: 181 NAVQFYSLKSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPV 240
            AV+FYS +SH Y HVLRFRS+VCMVRCS +IVAVGLATQIYCFDA+TLE  FSVLT PV
Sbjct: 181 TAVRFYSFQSHCYEHVLRFRSSVCMVRCSPRIVAVGLATQIYCFDALTLENKFSVLTYPV 240

Query: 241 PEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSD 300
           P+++GQG  G NVGYGPMAVGPRWLAY S   + S +  LS Q+  PS  V+P + PG  
Sbjct: 241 PQLAGQGAVGINVGYGPMAVGPRWLAYASNTLLLSNSGRLSPQNLTPS-GVSPSTSPGGS 300

Query: 301 RTKAHYPVK-SGWKAGRLAGMEADY--------------------PGMVAVKDFVT---- 360
              A Y ++ S   A  L+   + Y                     G  A  D       
Sbjct: 301 SLVARYAMEHSKQFAAGLSKTLSKYCQELLPDGSSSPVSPNSVWKVGRHAGADMDNAGIV 360

Query: 361 -------RAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPN-SRSGSSGL 420
                  RAII+QF+AHTSP+SALCFDPSGTLLVTAS+YGNNINIFRI+P+  RSGS   
Sbjct: 361 VVKDFVTRAIISQFKAHTSPISALCFDPSGTLLVTASVYGNNINIFRIMPSCMRSGSGNH 420

Query: 421 SFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRI 480
            +D NSSHVHLYKLHRGITSA IQDICFS+YSQW+AIVSSKGTCHVF+LSPFGG++G + 
Sbjct: 421 KYDWNSSHVHLYKLHRGITSATIQDICFSHYSQWIAIVSSKGTCHVFVLSPFGGDSGFQT 480

Query: 481 LNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSA 540
           L+SQG +P + PVL+LPWW TSS I  QQ   PPPPVTLSVVSRIKYSSFGWLNTV+N++
Sbjct: 481 LSSQGGDPYLFPVLSLPWWCTSSGISEQQCVLPPPPVTLSVVSRIKYSSFGWLNTVSNAS 540

Query: 541 GA--GKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAE 600
            +  GK FVPSGAVAA+FHN+++H+ QHVNS+ NSLEHLLVYTPSG+VVQHELLPS G  
Sbjct: 541 ASSMGKVFVPSGAVAAVFHNSIAHSSQHVNSRTNSLEHLLVYTPSGYVVQHELLPSIGMG 600

Query: 601 PSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTN 660
           PS   SR  ++S++ +QEDDL+++VEP+QWWD CRRSD+ ER E I ++T DG    +  
Sbjct: 601 PSDDGSRIRAASLMCLQEDDLQVRVEPVQWWDVCRRSDWPEREEFISEATCDGHGAVE-- 660

Query: 661 AIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEVQLSSGRLP 720
            I   ++D E  + +DF ++ND     E    +  S  ++E+SHW+LSNAEVQ+SSGRLP
Sbjct: 661 -IFQNKSDCEDNYGIDFLDIND--CIVEKSTFKNCSVKSYERSHWYLSNAEVQMSSGRLP 720

Query: 721 IWQNSKI--YVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRG 780
           IWQ+SKI  + M SPR N+ A GEFEIEKV V E+E++ KELLP FDH   +K   N+RG
Sbjct: 721 IWQSSKISFFKMDSPRANTHASGEFEIEKVSVHEVEIKRKELLPVFDHFQCIKPSWNNRG 780

Query: 781 LLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESSDGGSSRRMESSIDFDQAS 840
           L   +     SS  YQ+E K+ +   VICHS PASLSSTESS+GGSSRR+E+ +D DQ +
Sbjct: 781 LAEEKRPLSPSSGPYQAEDKIAQQ-TVICHSNPASLSSTESSEGGSSRRIENLLDLDQVN 840

Query: 841 CEKSCTPLCQHLNEMY---WEKRASESFTTPKSS-NILCTQVEGSRIDGSPCGLHFSNSN 900
            +K   P  Q LNE+Y    E    ES T  K   +I+ T  E S  D      H  N  
Sbjct: 841 NDKLYVPTGQTLNEIYNGRHEVTMVESSTLNKRCLDIVSTSPEHSENDNPHVNNHIPN-- 900

Query: 901 FGFPSIEQ---VSSKTPPFNEVTF-------QHI---------------------CQEEP 929
            G PS+E     + +      V+         H+                      +EE 
Sbjct: 901 -GLPSLESNLPSAGRDDTIVAVSMLGADYYDSHMGIIMEDRALPLLSCPVNLGVSLREEH 960

BLAST of Cucsa.084820 vs. TrEMBL
Match: A0A067H6H2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g001814mg PE=4 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 5.7e-272
Identity = 551/1013 (54.39%), Postives = 672/1013 (66.34%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKGRN GLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASIS AS ED KDQV
Sbjct: 1   MKKGKGRNNGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISNAS-EDLKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGFD LE  P + + +LLLGY NGFQ+LDVEDASNF ELVSKR GPVSFLQ+ P P K
Sbjct: 61  TWAGFDRLEYGPSVFKQVLLLGYQNGFQVLDVEDASNFNELVSKRDGPVSFLQMQPFPVK 120

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVL--------PGSCANSH 180
                   D  E  R+ HP LL+VAGE++  +A GQN S +G +         G+C NS 
Sbjct: 121 -------DDGCEGFRKLHPFLLVVAGEDTNTLAPGQNRSHLGGVRDGMMDSQSGNCVNSP 180

Query: 181 NAVQFYSLKSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPV 240
            AV+FYS +SH Y HVLRFRS+VCMVRCS +IVAVGLATQIYCFDA+TLE  FSVLT PV
Sbjct: 181 TAVRFYSFQSHCYEHVLRFRSSVCMVRCSPRIVAVGLATQIYCFDALTLENKFSVLTYPV 240

Query: 241 PEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSD 300
           P+++GQG  G NVGYGPMAVGPRWLAY S   + S +  LS Q+  PS  V+P + PG  
Sbjct: 241 PQLAGQGAVGINVGYGPMAVGPRWLAYASNTLLLSNSGRLSPQNLTPS-GVSPSTSPGGS 300

Query: 301 RTKAHYPVK-SGWKAGRLAGMEADY--------------------PGMVAVKDFVT---- 360
              A Y ++ S   A  L+   + Y                     G  A  D       
Sbjct: 301 SLVARYAMEHSKQFAAGLSKTLSKYCQELLPDGSSSPVSPNSVWKVGRHAGADMDNAGIV 360

Query: 361 -------RAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPN-SRSGSSGL 420
                  RAII+QF+AHTSP+SALCFDPSGTLLVTAS+YGNNINIFRI+P+  RSGS   
Sbjct: 361 VVKDFVTRAIISQFKAHTSPISALCFDPSGTLLVTASVYGNNINIFRIMPSCMRSGSGNH 420

Query: 421 SFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRI 480
            +D NSSHVHLYKLHRGITSA IQDICFS+YSQW+AIVSSKGTCHVF+LSPFGG++G + 
Sbjct: 421 KYDWNSSHVHLYKLHRGITSATIQDICFSHYSQWIAIVSSKGTCHVFVLSPFGGDSGFQT 480

Query: 481 LNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSA 540
           L+SQG +P + PVL+LPWW TSS I  QQ   PPPPVTLSVVSRIKYSSFGWLNTV+N++
Sbjct: 481 LSSQGGDPYLFPVLSLPWWCTSSGISEQQCVLPPPPVTLSVVSRIKYSSFGWLNTVSNAS 540

Query: 541 GA--GKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAE 600
            +  GK FVPSGAVAA+FHN+++H+ QHVNS+ NSLEHLLVYTPSG+VVQHELLPS G  
Sbjct: 541 ASSMGKVFVPSGAVAAVFHNSIAHSSQHVNSRTNSLEHLLVYTPSGYVVQHELLPSIGMG 600

Query: 601 PSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTN 660
           PS   SR  ++S++ +QEDDL+++VEP+QWWD CRRSD+ ER E I ++T DG    +  
Sbjct: 601 PSDDGSRIRAASLMCLQEDDLQVRVEPVQWWDVCRRSDWPEREEFISEATCDGHGAVE-- 660

Query: 661 AIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEVQLSSGRLP 720
            I   ++D E  + +DF ++ND     E    +  S  ++E+SHW+LSNAEVQ+SSGRLP
Sbjct: 661 -IFQNKSDCEDNYGIDFLDIND--CIVEKSTFKNCSVKSYERSHWYLSNAEVQMSSGRLP 720

Query: 721 IWQNSKI--YVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRG 780
           IWQ+SKI  + M SPR N+ A GEFEIEKV V E+E++ KELLP FDH   +K   N+RG
Sbjct: 721 IWQSSKISFFKMDSPRANTHASGEFEIEKVSVHEVEIKRKELLPVFDHFQCIKPSWNNRG 780

Query: 781 LLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESSDGGSSRRMESSIDFDQAS 840
           L   +     SS  YQ+E K+ +   VICHS PASLSSTESS+GGSSRR+E+ +D DQ +
Sbjct: 781 LAEEKRPLSPSSGPYQAEDKIAQQ-TVICHSNPASLSSTESSEGGSSRRIENLLDLDQVN 840

Query: 841 CEKSCTPLCQHLNEMY---WEKRASESFTTPKSS-NILCTQVEGSRIDGSPCGLHFSNSN 900
            +K   P  Q LNE+Y    E    ES T  K   +I+ T  E S  D      H  N  
Sbjct: 841 NDKLYVPTGQTLNEIYNGRHEVTMVESSTLNKRCLDIVSTSPEHSENDNPHVNNHIPN-- 900

Query: 901 FGFPSIEQ---VSSKTPPFNEVTF-------QHI---------------------CQEEP 929
            G PS+E     + +      V+         H+                      +EE 
Sbjct: 901 -GLPSLESNLPSAGRDDTIVAVSMLGADYYDSHMGIIMEDRALPLLSCPVNLGVSLREEH 960

BLAST of Cucsa.084820 vs. TrEMBL
Match: V4TV77_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018690mg PE=4 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 9.7e-272
Identity = 551/1013 (54.39%), Postives = 671/1013 (66.24%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKGRN GLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASIS AS ED KDQV
Sbjct: 1   MKKGKGRNNGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISNAS-EDLKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGFD LE  P + + +LLLGY NGFQ+LDVEDASNF ELVSKR GPVSFLQ+ P P K
Sbjct: 61  TWAGFDRLEYGPSVFKQVLLLGYQNGFQVLDVEDASNFNELVSKRDGPVSFLQMQPFPVK 120

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVL--------PGSCANSH 180
                   D  E  R+ HP LL+VAGE++  +A GQN S +G +         G+C NS 
Sbjct: 121 -------DDGCEGFRKLHPFLLVVAGEDTNTLAPGQNRSHLGGVRDGMMDSQSGNCVNSP 180

Query: 181 NAVQFYSLKSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPV 240
            AV+FYS +SH Y HVLRFRS+VCMVRCS +IVAVGLATQIYCFDA+TLE  FSVLT PV
Sbjct: 181 TAVRFYSFQSHCYEHVLRFRSSVCMVRCSPRIVAVGLATQIYCFDALTLENKFSVLTYPV 240

Query: 241 PEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSD 300
           P+++GQG  G NVGYGPMAVGPRWLAY S   + S +  LS Q+  PS  V+P + PG  
Sbjct: 241 PQLAGQGAVGINVGYGPMAVGPRWLAYASNTLLLSNSGRLSPQNLTPS-GVSPSTSPGGS 300

Query: 301 RTKAHYPVK-SGWKAGRLAGMEADY--------------------PGMVAVKDFVT---- 360
              A Y ++ S   A  L+   + Y                     G  A  D       
Sbjct: 301 SLVARYAMEHSKQFAAGLSKTLSKYCQELLPDGSSSPVSPNSVWKVGRHAGADMDNAGIV 360

Query: 361 -------RAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPN-SRSGSSGL 420
                  RAII+QF+AHTSP+SALCFDPSGTLLVTAS+YGNNINIFRI+P+  RSGS   
Sbjct: 361 VVKDFVTRAIISQFKAHTSPISALCFDPSGTLLVTASVYGNNINIFRIMPSCMRSGSGNH 420

Query: 421 SFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRI 480
            +D NSSHVHLYKLHRGITSA IQDICFS+YSQW+AIVSSKGTCHVF+LSPFGG++G + 
Sbjct: 421 KYDWNSSHVHLYKLHRGITSATIQDICFSHYSQWIAIVSSKGTCHVFVLSPFGGDSGFQT 480

Query: 481 LNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSA 540
           L+SQG +P + PVL+LPWW TSS I  QQ   PPPPVTLSVVSRIKYSSFGWLNTV+N++
Sbjct: 481 LSSQGGDPYLFPVLSLPWWCTSSGISEQQCVLPPPPVTLSVVSRIKYSSFGWLNTVSNAS 540

Query: 541 GA--GKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAE 600
            +  GK FVPSGAVAA+FHN+++H+ QHVNS+ NSLEHLLVYTPSG+VVQHELLPS G  
Sbjct: 541 ASSMGKVFVPSGAVAAVFHNSIAHSSQHVNSRTNSLEHLLVYTPSGYVVQHELLPSIGMG 600

Query: 601 PSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTN 660
           PS   SR  ++S++ +QEDDL+++VEP+QWWD CRRSD+ ER E I +ST DG    +  
Sbjct: 601 PSDDGSRIRAASLMCLQEDDLQVRVEPVQWWDVCRRSDWPEREEFISESTCDGHGAVE-- 660

Query: 661 AIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEVQLSSGRLP 720
            I   ++D E  + +DF ++ND     E    +  S  ++E+SHW+LSNAEVQ+SSGRLP
Sbjct: 661 -IFQNKSDCEDNYGIDFLDIND--CIVEKSTFKNCSVKSYERSHWYLSNAEVQMSSGRLP 720

Query: 721 IWQNSKI--YVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRG 780
           IWQ+SKI  + M SPR N+ A GEFEIEKV V E+E++ KELLP FDH   +K   N+RG
Sbjct: 721 IWQSSKISFFKMDSPRANTHASGEFEIEKVSVHEVEIKRKELLPVFDHFQCIKPSWNNRG 780

Query: 781 LLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESSDGGSSRRMESSIDFDQAS 840
           L   +     SS  YQ+E K+ +   VICHS PASLSSTESS+GGSSRR+E+ +D DQ +
Sbjct: 781 LAEEKRPLSPSSGPYQAEDKIAQQ-TVICHSNPASLSSTESSEGGSSRRIENLLDLDQVN 840

Query: 841 CEKSCTPLCQHLNEMY---WEKRASESFTTPKSS-NILCTQVEGSRIDGSPCGLHFSNSN 900
            +K   P  Q LNE+Y    E    ES T  K   +I+    E S  D      H  N  
Sbjct: 841 NDKLYVPTGQTLNEIYNGRHEVTMVESSTLNKRCLDIVSASPEHSENDNPHVNNHIPN-- 900

Query: 901 FGFPSIEQ---VSSKTPPFNEVTF-------QHI---------------------CQEEP 929
            G PS+E     + +      V+         H+                      +EE 
Sbjct: 901 -GLPSLESNLPSAGRDDTIVAVSMLGADYYDSHMGIIMEDRALPLLSCPVNLGVSLREEH 960

BLAST of Cucsa.084820 vs. TAIR10
Match: AT1G03380.1 (AT1G03380.1 homolog of yeast autophagy 18 (ATG18) G)

HSP 1 Score: 511.9 bits (1317), Expect = 8.2e-145
Identity = 297/626 (47.44%), Postives = 404/626 (64.54%), Query Frame = 1

Query: 304 WKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNI 363
           WK G ++G +A+  GMVAVKD V+ A+++QF+AHTSP+SALCFDPSGTLLVTAS+ GNNI
Sbjct: 344 WKVGGVSGSDAENAGMVAVKDLVSGALVSQFKAHTSPISALCFDPSGTLLVTASVCGNNI 403

Query: 364 NIFRIIPNSRSGSSG-LSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGT 423
           N+F+I+P+    + G LS++  SSHVHL+KLHRGITSA++QDICFS  SQWVAI+SSKGT
Sbjct: 404 NVFQIMPSRSHNAPGDLSYEWESSHVHLFKLHRGITSAIVQDICFSQQSQWVAIISSKGT 463

Query: 424 CHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVS 483
           CH+F+L+  G +A  +    +GEEP  LP  +LPWW T SL  NQQS  PP  V LSVVS
Sbjct: 464 CHIFVLNSSGSDAAFQ--PCEGEEPTRLPASSLPWWFTQSLSSNQQSLSPPTAVALSVVS 523

Query: 484 RIKYSSFGWLNTVNNS--AGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYT 543
           RIKYSSFGWLNTV+N+  A  GK FVPSGAVAA+FH +++H++Q +NS+ N+LEH+LVYT
Sbjct: 524 RIKYSSFGWLNTVSNATTAATGKVFVPSGAVAAVFHKSVTHDLQ-LNSRTNALEHILVYT 583

Query: 544 PSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERG 603
           PSGHVVQHELLPS   E   +  R + +S + +QEDDL++KVEPIQWWD CRRSD+ E  
Sbjct: 584 PSGHVVQHELLPSVCTESPENGLRVQKTSHVQVQEDDLRVKVEPIQWWDVCRRSDWLETE 643

Query: 604 ECIHDSTSDGQDIAKTNAIQNERTDTEKT-HELDFQEMNDGSSADEILRVRGQSGITHEQ 663
           E +  S ++ Q   +T  + N  T  E     LD   MN   S D+ L+   +     E+
Sbjct: 644 ERLPKSITEKQYDLET--VSNHLTSHEDACLSLD---MNSHFSEDKYLKSCSEK--PPER 703

Query: 664 SHWFLSNAEVQLSSGRLPIWQNSKI--YVMSSPRINSIAGGEFEIEKVPVQEIEVREKEL 723
           SH +LSN EV+++SG LP+WQNSKI  +VM SPR +S  GGEFEIEKVP  E+E+++K+L
Sbjct: 704 SHCYLSNFEVKVTSGMLPVWQNSKISFHVMDSPRDSSSTGGEFEIEKVPAHELEIKQKKL 763

Query: 724 LPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESS 783
           LP FDH  S K+   DR  +  +C   +++  +Q   K+ + I + CHSKP S+ S ESS
Sbjct: 764 LPVFDHFHSTKATLEDRFSM--KCYHTSATGSHQVNGKICQDI-INCHSKPGSIESAESS 823

Query: 784 DGGSSRRMESSIDFDQASCE-KSCTPLCQHLNEMY--WEKRASESFTTPKSSNILCTQVE 843
           + GS+++ME+  D D  S   KS  PL   +N +Y   EK  +  +     +  L T  E
Sbjct: 824 EEGSTKQMENLHDSDHMSNSIKSSLPLYPTVNGIYKEIEKNNANGWMEKPVTAKLSTLKE 883

Query: 844 GSRIDGSPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVLESNDGCQDIN 903
               +G       ++S     + + +S+  PP     F     EE CK +   D  ++  
Sbjct: 884 TRITNGFTTPPILTDS----VNEQMLSTGKPPMG---FGFALHEEHCKAVA--DPKEEHL 943

Query: 904 DISTDHVDGFEYENICSDENDKIFGD 921
               D V    + N+ ++  +K+ GD
Sbjct: 944 KKKLDEVTNVHHLNVNNNNTEKLQGD 947

HSP 2 Score: 352.1 bits (902), Expect = 1.1e-96
Identity = 192/311 (61.74%), Postives = 223/311 (71.70%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKG+N GLLPNS KIISSCLKTVS NA+ VAS+VRSAGASVAASISAA  ED+KDQV
Sbjct: 2   MKKGKGKNSGLLPNSFKIISSCLKTVSANATNVASSVRSAGASVAASISAA--EDDKDQV 61

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGF ILE+  ++ RH+LLLGY NGFQ+ DVEDASNF ELVSKRGGPVSFLQ+ P PA+
Sbjct: 62  TWAGFGILELGQHVTRHVLLLGYQNGFQVFDVEDASNFNELVSKRGGPVSFLQMQPLPAR 121

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLP---------GSCANS 180
            G        HE    SHPLLL+VAG+E+    +G + S  G L          G   N 
Sbjct: 122 SGD-------HEGFWNSHPLLLVVAGDETNGTGLGHSFSQNGSLARDGSSDSKAGDAINY 181

Query: 181 HNAVQFYSLKSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSP 240
              V+FYSL+SHSYV+VLRFRS+VCM+RCSS++VAVGLA QIYC DA+TLE  FSVLT P
Sbjct: 182 PTTVRFYSLRSHSYVYVLRFRSSVCMIRCSSRVVAVGLANQIYCVDALTLENKFSVLTYP 241

Query: 241 VPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGS 300
           VP+   QGTT  NVGYGPMAVGPRWLAY S   +   T  LS Q+   S S++P S  G 
Sbjct: 242 VPQPVRQGTTRVNVGYGPMAVGPRWLAYASKSSMTMKTGRLSPQTFTSSPSLSPSSSSGG 301

Query: 301 DRTKAHYPVKS 303
               A Y ++S
Sbjct: 302 SSFMARYAMES 303

BLAST of Cucsa.084820 vs. TAIR10
Match: AT1G54710.1 (AT1G54710.1 homolog of yeast autophagy 18 (ATG18) H)

HSP 1 Score: 381.3 bits (978), Expect = 1.7e-105
Identity = 222/464 (47.84%), Postives = 295/464 (63.58%), Query Frame = 1

Query: 303 GWKAGRLAG--MEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYG 362
           G K GR+     E+D  G V VKDF +RAII QFRAHTSP+SALCFDPSGTLLVTASI+G
Sbjct: 344 GRKVGRVGSHSAESDVVGTVIVKDFESRAIIAQFRAHTSPISALCFDPSGTLLVTASIHG 403

Query: 363 NNINIFRIIPN-SRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSS 422
           NNIN+FRI+P  +++G    S+D +SSHV LYKLHRG+TSA+IQDICFS+YSQW+AIVSS
Sbjct: 404 NNINVFRIMPTPTKNGPGAQSYDWSSSHVPLYKLHRGMTSAVIQDICFSSYSQWIAIVSS 463

Query: 423 KGTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLS 482
           K TCH+++LSPFGGE  L I NSQ + P + P L+LPWWS+ S +    S+PPP  VTLS
Sbjct: 464 KSTCHIYVLSPFGGENVLEIRNSQFDGPTLAPTLSLPWWSSPSFMTTHFSYPPPASVTLS 523

Query: 483 VVSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVY 542
           VVSRIK ++F        S+  GK   PSG +AA+FH ++    Q   S   +L++LLVY
Sbjct: 524 VVSRIKCNNFFHA----ASSVVGKPTFPSGCLAAVFHQSVP---QESQSSSPALDYLLVY 583

Query: 543 TPSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSER 602
           TPSGHVVQ++L+PS G + +  ++R  ++S L   E++L++KVEP+Q WD CRR+D+ ER
Sbjct: 584 TPSGHVVQYKLIPSLGGDQAESNTRNGATSGL-TSEEELRVKVEPVQCWDVCRRADWPER 643

Query: 603 GECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQ 662
            E I   T  G+  A+     ++  D  K                             E+
Sbjct: 644 EENICGLTYGGRKNAELTVDTSDSEDQTK---------------------------PLEK 703

Query: 663 SHWFLSNAEVQLSSGRLPIWQNSKI--YVMSSP-----RINS-IAGGEFEIEKVPVQEIE 722
            H +L+NAEV ++SGR PIWQNS+I  Y M  P      +NS   GGE EI KV   E++
Sbjct: 704 HHVYLANAEVLINSGRKPIWQNSEISFYPMYPPDSDGKNLNSHQGGGETEIGKVSANEVD 763

Query: 723 VREKELLPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVK 756
           +R K+LLP +D+  S+ +   +RG   G   S +SS     +VK
Sbjct: 764 IRRKDLLPVYDNFHSVYTSMRNRG-FSGERDSDSSSSSDPGQVK 771

HSP 2 Score: 259.6 bits (662), Expect = 7.3e-69
Identity = 153/302 (50.66%), Postives = 193/302 (63.91%), Query Frame = 1

Query: 3   KGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQVTW 62
           K  G N G LPNSLK IS+C++T S       S VRSA ASVAAS+S+ S E  KDQV W
Sbjct: 20  KNNGTN-GFLPNSLKFISTCIRTAS-------SGVRSASASVAASLSSDSHE-LKDQVLW 79

Query: 63  AGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAKPG 122
           + FD L       +++LLLGY NGFQ+LD++D+++  E VS+R  PV+FLQ+ P PAK  
Sbjct: 80  SSFDRLHTSESSFKNVLLLGYTNGFQVLDIDDSNDVTEFVSRRDDPVTFLQMQPLPAK-- 139

Query: 123 TPPAKSDRHELLRRSHPLLLIVAGEE--SKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 182
                 D  E  R SHP+LL VA E   S  +   ++ S         A S   V+FYSL
Sbjct: 140 -----CDGVEGFRSSHPILLAVADEAKGSGPIVTSRDGSVRNGYEDPLALSPTVVRFYSL 199

Query: 183 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT 242
           +SH+YVHVLRFRS V MVRCS +IVAVGL +QIYCFDA+TLE  FSVL+ PVP++  QG 
Sbjct: 200 RSHNYVHVLRFRSTVYMVRCSPRIVAVGLGSQIYCFDALTLENKFSVLSYPVPQLGNQGI 259

Query: 243 TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV 302
           +G NVGYGPMAVG RWLAY S  P+ S+   LS Q+  P   V+P + P +    A Y +
Sbjct: 260 SGVNVGYGPMAVGARWLAYASNSPLSSSIGRLSPQNVTP-PGVSPSTSPNNGNLVARYAM 304

BLAST of Cucsa.084820 vs. TAIR10
Match: AT5G54730.1 (AT5G54730.1 homolog of yeast autophagy 18 (ATG18) F)

HSP 1 Score: 310.1 bits (793), Expect = 4.7e-84
Identity = 260/823 (31.59%), Postives = 381/823 (46.29%), Query Frame = 1

Query: 14  NSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQVTWAGFDILEVEPY 73
           +S + +S+CLK +S+ ASTVA +  SA +S          E   DQV WAGFD L+ E  
Sbjct: 23  SSFRALSNCLKVISSGASTVARSAVSAASSAV--------ESHHDQVLWAGFDNLQKEDG 82

Query: 74  IIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAKPGTPPAKSDRHEL 133
             R +LLL + +GFQ+ DVED  N   +VS   G   F+Q+L +P   G      DR   
Sbjct: 83  DTRRVLLLAFKSGFQVWDVEDTENVHVIVSAHDGQAFFMQMLLNPINSGV---LDDR--- 142

Query: 134 LRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSLKSHSYVHVLRFRS 193
             +S PLL +     SK ++     S     P +       V  YSLKS SYVH L+FR+
Sbjct: 143 FYKSRPLLAVCGDYSSKKISSDNPGSETVATPTN-------VYVYSLKSQSYVHTLKFRA 202

Query: 194 AVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGTTGTNVGYGPMAVG 253
            +  VRC S+IVAV  A QI CFDA TLE+ + ++T+ +      G+ G  VGYGP+AVG
Sbjct: 203 TIYSVRCCSRIVAVLQAAQIDCFDAATLEMDYRIVTNSIV----CGSLG--VGYGPLAVG 262

Query: 254 PRWLAYP-------SVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSD----------RTKA 313
           PRW+AY        S     S  V LS+ SP  +Q     S   +           R+  
Sbjct: 263 PRWIAYSGSRIATSSSAIFTSEIVSLSTSSPSVAQFARDSSKQLASGIANLGDKGYRSLT 322

Query: 314 HY----------PVKSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCF 373
            Y          P   G   G     +A+  GMV VKD   +++ITQF+AH SP+SALCF
Sbjct: 323 KYCSEVLPNPYIPGLKGIGVGNEKVADAESIGMVIVKDITNKSVITQFKAHKSPISALCF 382

Query: 374 DPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDIC 433
           D SG LLVTASI G+NIN+FRI+P   +  +  +    ++  HL++L RG T+A+IQDIC
Sbjct: 383 DQSGLLLVTASIQGHNINVFRIMPTISTSRAVKT----TTFAHLFRLQRGFTNAVIQDIC 442

Query: 434 FSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIIN 493
           FS  S  + + SS+GT H+F ++P            +G+ P                   
Sbjct: 443 FSKDSNLIVVGSSRGTSHLFEINP----------EKEGDAPV------------------ 502

Query: 494 QQSFPPPPPVTLSVVSRIKY-SSFGWLNTVNNSAGAGKGFVPS---GAVAAIF------- 553
                      +S +SRI+  +S GW+ TV+ +A A  G V     G V + F       
Sbjct: 503 ----------PMSAISRIRSGNSSGWIGTVSGAASAAAGMVAGSVPGTVTSTFCYCDEKS 562

Query: 554 HNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLP---SFGAEPSLHSSRTESSSVLH 613
           +N    ++  + SK N    LLV+ PSG + Q+ L       G E +  +     S +  
Sbjct: 563 NNNYYGSVADMCSKTN----LLVFAPSGCMTQYALREHQVGVGHETAAMTGFDSESGL-- 622

Query: 614 MQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQNERTDTEKTHEL 673
             E + KL V+PI+ W   +     E     HD  SD         I    T  +   ++
Sbjct: 623 --ETEGKLAVDPIRRWSMIQNQSRRE----THDPHSD---------IYGGGTSVDSKSKV 682

Query: 674 DFQEMNDGSSADEILRVRGQSGITH--EQSHWFLSNAEVQLS--SGRLPIWQNSK----- 733
            F E+    S +E  +V  + G T   ++ H ++  AE Q +    +LP+W   K     
Sbjct: 683 -FPEVVRKQSVEEAWKV-SKKGTTRVVDKRHLYIYEAEQQQTHLPTQLPLWARRKFRFQE 742

Query: 734 IYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRCIS 787
           + +     I+   G E EIE +  + IE R ++L+P + +  S +S       ++   I 
Sbjct: 743 LVLNRGEEISGGGGREMEIEGIQTRTIEARTRDLVPVWGYLQSPRS-----QQVINESIQ 743

BLAST of Cucsa.084820 vs. TAIR10
Match: AT5G05150.1 (AT5G05150.1 autophagy-related gene 18E)

HSP 1 Score: 55.5 bits (132), Expect = 2.1e-07
Identity = 37/115 (32.17%), Postives = 52/115 (45.22%), Query Frame = 1

Query: 316 YPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPNSRSG 375
           +PG V V D     +I   +AH S ++ +     G+LL TAS  G  I IF  +  +   
Sbjct: 183 HPGQVQVHDLRWN-VIKFIKAHDSAIACMTLTLDGSLLATASTKGTLIRIFNAVDGTL-- 242

Query: 376 SSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSP 431
                         L +  RG+  A I ++  S+  +WVA  S KGT HVF L P
Sbjct: 243 --------------LQEFRRGVERAEIYNVAISSNLKWVAASSEKGTLHVFRLRP 280

BLAST of Cucsa.084820 vs. NCBI nr
Match: gi|700189617|gb|KGN44850.1| (hypothetical protein Csa_7G391270 [Cucumis sativus])

HSP 1 Score: 1876.7 bits (4860), Expect = 0.0e+00
Identity = 927/928 (99.89%), Postives = 927/928 (99.89%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV
Sbjct: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK
Sbjct: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 180
           PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL
Sbjct: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 180

Query: 181 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT 240
           KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT
Sbjct: 181 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT 240

Query: 241 TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV 300
           TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV
Sbjct: 241 TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV 300

Query: 301 KSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYG 360
           KSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYG
Sbjct: 301 KSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYG 360

Query: 361 NNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSK 420
           NNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSK
Sbjct: 361 NNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSK 420

Query: 421 GTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSV 480
           GTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSV
Sbjct: 421 GTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSV 480

Query: 481 VSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYT 540
           VSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYT
Sbjct: 481 VSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYT 540

Query: 541 PSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERG 600
           PSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERG
Sbjct: 541 PSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERG 600

Query: 601 ECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQS 660
           ECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQS
Sbjct: 601 ECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQS 660

Query: 661 HWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPC 720
           HWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPC
Sbjct: 661 HWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPC 720

Query: 721 FDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESSDGG 780
           FDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTE IVVICHSKPASLSSTESSDGG
Sbjct: 721 FDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTEEIVVICHSKPASLSSTESSDGG 780

Query: 781 SSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDG 840
           SSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDG
Sbjct: 781 SSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDG 840

Query: 841 SPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDH 900
           SPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDH
Sbjct: 841 SPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDH 900

Query: 901 VDGFEYENICSDENDKIFGDMFTSSEEG 929
           VDGFEYENICSDENDKIFGDMFTSSEEG
Sbjct: 901 VDGFEYENICSDENDKIFGDMFTSSEEG 928

BLAST of Cucsa.084820 vs. NCBI nr
Match: gi|778727809|ref|XP_011659321.1| (PREDICTED: autophagy-related protein 18g isoform X2 [Cucumis sativus])

HSP 1 Score: 1866.3 bits (4833), Expect = 0.0e+00
Identity = 927/944 (98.20%), Postives = 927/944 (98.20%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV
Sbjct: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK
Sbjct: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 180
           PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL
Sbjct: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 180

Query: 181 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQ----------------IYCFDAITLEVV 240
           KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQ                IYCFDAITLEVV
Sbjct: 181 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQVRTKMTDNYGYRIDLMIYCFDAITLEVV 240

Query: 241 FSVLTSPVPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVN 300
           FSVLTSPVPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVN
Sbjct: 241 FSVLTSPVPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVN 300

Query: 301 PPSLPGSDRTKAHYPVKSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSAL 360
           PPSLPGSDRTKAHYPVKSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSAL
Sbjct: 301 PPSLPGSDRTKAHYPVKSGWKAGRLAGMEADYPGMVAVKDFVTRAIITQFRAHTSPLSAL 360

Query: 361 CFDPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQD 420
           CFDPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQD
Sbjct: 361 CFDPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSFDCNSSHVHLYKLHRGITSAMIQD 420

Query: 421 ICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLI 480
           ICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLI
Sbjct: 421 ICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILNSQGEEPCVLPVLTLPWWSTSSLI 480

Query: 481 INQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQ 540
           INQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQ
Sbjct: 481 INQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAGAGKGFVPSGAVAAIFHNTLSHNIQ 540

Query: 541 HVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEP 600
           HVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEP
Sbjct: 541 HVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAEPSLHSSRTESSSVLHMQEDDLKLKVEP 600

Query: 601 IQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSAD 660
           IQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSAD
Sbjct: 601 IQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQNERTDTEKTHELDFQEMNDGSSAD 660

Query: 661 EILRVRGQSGITHEQSHWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEK 720
           EILRVRGQSGITHEQSHWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEK
Sbjct: 661 EILRVRGQSGITHEQSHWFLSNAEVQLSSGRLPIWQNSKIYVMSSPRINSIAGGEFEIEK 720

Query: 721 VPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTEGIVVIC 780
           VPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTE IVVIC
Sbjct: 721 VPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQSEVKVTEEIVVIC 780

Query: 781 HSKPASLSSTESSDGGSSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPK 840
           HSKPASLSSTESSDGGSSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPK
Sbjct: 781 HSKPASLSSTESSDGGSSRRMESSIDFDQASCEKSCTPLCQHLNEMYWEKRASESFTTPK 840

Query: 841 SSNILCTQVEGSRIDGSPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVL 900
           SSNILCTQVEGSRIDGSPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVL
Sbjct: 841 SSNILCTQVEGSRIDGSPCGLHFSNSNFGFPSIEQVSSKTPPFNEVTFQHICQEEPCKVL 900

Query: 901 ESNDGCQDINDISTDHVDGFEYENICSDENDKIFGDMFTSSEEG 929
           ESNDGCQDINDISTDHVDGFEYENICSDENDKIFGDMFTSSEEG
Sbjct: 901 ESNDGCQDINDISTDHVDGFEYENICSDENDKIFGDMFTSSEEG 944

BLAST of Cucsa.084820 vs. NCBI nr
Match: gi|449462290|ref|XP_004148874.1| (PREDICTED: autophagy-related protein 18g isoform X1 [Cucumis sativus])

HSP 1 Score: 1857.4 bits (4810), Expect = 0.0e+00
Identity = 927/967 (95.86%), Postives = 927/967 (95.86%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV
Sbjct: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120
           TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK
Sbjct: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPAK 120

Query: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 180
           PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL
Sbjct: 121 PGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLPGSCANSHNAVQFYSL 180

Query: 181 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT 240
           KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT
Sbjct: 181 KSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTSPVPEISGQGT 240

Query: 241 TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV 300
           TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV
Sbjct: 241 TGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPGSDRTKAHYPV 300

Query: 301 K---------------------------------------SGWKAGRLAGMEADYPGMVA 360
           K                                       SGWKAGRLAGMEADYPGMVA
Sbjct: 301 KSGKQLAAGIFNLGGMGYKTWSNYYQDLNLNKYNFLIESNSGWKAGRLAGMEADYPGMVA 360

Query: 361 VKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSF 420
           VKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSF
Sbjct: 361 VKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSF 420

Query: 421 DCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILN 480
           DCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILN
Sbjct: 421 DCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILN 480

Query: 481 SQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAGA 540
           SQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAGA
Sbjct: 481 SQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAGA 540

Query: 541 GKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAEPSLH 600
           GKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAEPSLH
Sbjct: 541 GKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAEPSLH 600

Query: 601 SSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQN 660
           SSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQN
Sbjct: 601 SSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQN 660

Query: 661 ERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEVQLSSGRLPIWQN 720
           ERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEVQLSSGRLPIWQN
Sbjct: 661 ERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEVQLSSGRLPIWQN 720

Query: 721 SKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRC 780
           SKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRC
Sbjct: 721 SKIYVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRC 780

Query: 781 ISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESSDGGSSRRMESSIDFDQASCEKSCT 840
           ISPTSSEIYQSEVKVTE IVVICHSKPASLSSTESSDGGSSRRMESSIDFDQASCEKSCT
Sbjct: 781 ISPTSSEIYQSEVKVTEEIVVICHSKPASLSSTESSDGGSSRRMESSIDFDQASCEKSCT 840

Query: 841 PLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDGSPCGLHFSNSNFGFPSIEQVS 900
           PLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDGSPCGLHFSNSNFGFPSIEQVS
Sbjct: 841 PLCQHLNEMYWEKRASESFTTPKSSNILCTQVEGSRIDGSPCGLHFSNSNFGFPSIEQVS 900

Query: 901 SKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDHVDGFEYENICSDENDKIFGDM 929
           SKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDHVDGFEYENICSDENDKIFGDM
Sbjct: 901 SKTPPFNEVTFQHICQEEPCKVLESNDGCQDINDISTDHVDGFEYENICSDENDKIFGDM 960

BLAST of Cucsa.084820 vs. NCBI nr
Match: gi|659101518|ref|XP_008451647.1| (PREDICTED: autophagy-related protein 18g [Cucumis melo])

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 677/717 (94.42%), Postives = 696/717 (97.07%), Query Frame = 1

Query: 212 QIYCFDAITLEVVFSVLTSPVPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVP 271
           QIYCFDAITLEVVFSVLTSPVPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVP
Sbjct: 147 QIYCFDAITLEVVFSVLTSPVPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVP 206

Query: 272 LSSQSPFPSQSVNPPSLPGSDRTKAHYPVKSGWKAGRLAGMEADYPGMVAVKDFVTRAII 331
           LSSQ  FPSQ VNP SLPGSDRTKAHYPVKSGWKAGR  G+E DYPGMVAVKDF+TRAII
Sbjct: 207 LSSQCLFPSQGVNPSSLPGSDRTKAHYPVKSGWKAGRRVGVETDYPGMVAVKDFLTRAII 266

Query: 332 TQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPNSRSGSSGLSFDCNSSHVHLY 391
           TQFRAHTSP+SALCFDPSGTLLVTASIYGNNINIF+IIPNSRSGSSGLSFDCNSSHVHLY
Sbjct: 267 TQFRAHTSPISALCFDPSGTLLVTASIYGNNINIFQIIPNSRSGSSGLSFDCNSSHVHLY 326

Query: 392 KLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGLRILNSQGEEPCVLP 451
           KLHRGITSAMIQDICFSNYSQWVAIVS+KGTCHVFLLSPFGGEAG RILNSQGEEPC+LP
Sbjct: 327 KLHRGITSAMIQDICFSNYSQWVAIVSAKGTCHVFLLSPFGGEAGFRILNSQGEEPCLLP 386

Query: 452 VLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAGAGKGFVPSGAV 511
           VLTLPWWSTSSL+INQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAG+GKGFVPSGAV
Sbjct: 387 VLTLPWWSTSSLMINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNNSAGSGKGFVPSGAV 446

Query: 512 AAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFGAEPSLHSSRTESSSVL 571
           AAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFG EPSLHSSRTESSS L
Sbjct: 447 AAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFGVEPSLHSSRTESSSFL 506

Query: 572 HMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAKTNAIQNERTDTEKTHE 631
           HMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDS+SDGQD+AKT  IQNERTDTE+THE
Sbjct: 507 HMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSSSDGQDVAKT--IQNERTDTEETHE 566

Query: 632 LDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEVQLSSGRLPIWQNSKIYVMSSPR 691
           LDFQE+NDGSSADE+LRVRG+SGITHEQSHWFLSNAEVQLSSGR+PIWQNSKIYVMSSPR
Sbjct: 567 LDFQEINDGSSADEVLRVRGRSGITHEQSHWFLSNAEVQLSSGRVPIWQNSKIYVMSSPR 626

Query: 692 INSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQ 751
           INSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQ
Sbjct: 627 INSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCNDRGLLLGRCISPTSSEIYQ 686

Query: 752 SEVKVTEGIVVICHSKPASLSSTESSDGGSSRRMESSIDFDQASCEKSCTPLCQHLNEMY 811
           SEVKVTE IVVICHSKPASLSSTESSDGGSSRRMES IDFDQASCEKSCTPLCQHLNEMY
Sbjct: 687 SEVKVTEEIVVICHSKPASLSSTESSDGGSSRRMESFIDFDQASCEKSCTPLCQHLNEMY 746

Query: 812 WEKRASESFTTPKSSNILCTQVEGSRIDGSPCGLHFSNSNFGFPSIEQVSSKTPPFNEVT 871
           WEKRA+ESFTTPKSSNIL   VEGSRIDGSPC LHFSNS+FGFPSIEQVSSKTPPFNEVT
Sbjct: 747 WEKRATESFTTPKSSNIL---VEGSRIDGSPCDLHFSNSDFGFPSIEQVSSKTPPFNEVT 806

Query: 872 FQHICQEEPCKVLESNDGCQDINDISTDHVDGFEYENICSDENDKIFGDMFTSSEEG 929
           F+HICQEEPCKVLE+N GCQDINDISTDHVD  EYENICSDENDKIFGDMFTSSEEG
Sbjct: 807 FEHICQEEPCKVLEANVGCQDINDISTDHVDSVEYENICSDENDKIFGDMFTSSEEG 858

BLAST of Cucsa.084820 vs. NCBI nr
Match: gi|659101518|ref|XP_008451647.1| (PREDICTED: autophagy-related protein 18g [Cucumis melo])

HSP 1 Score: 201.4 bits (511), Expect = 6.7e-48
Identity = 104/106 (98.11%), Postives = 105/106 (99.06%), Query Frame = 1

Query: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60
           MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV
Sbjct: 1   MKKGKGRNLGLLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQV 60

Query: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRG 107
           TWAGFDILEVEPYIIRHILLLGYLNGFQ+LDVED SNFKELVSKRG
Sbjct: 61  TWAGFDILEVEPYIIRHILLLGYLNGFQVLDVEDDSNFKELVSKRG 106

HSP 2 Score: 1013.1 bits (2618), Expect = 3.2e-292
Identity = 544/881 (61.75%), Postives = 652/881 (74.01%), Query Frame = 1

Query: 1   MKKGKGRNLG-LLPNSLKIISSCLKTVSTNASTVASTVRSAGASVAASISAASSEDEKDQ 60
           MKKGKG+N G LLPNSL+IISSCLKTVSTNAS VASTVRSAGASVAA+ISAASSED+KDQ
Sbjct: 1   MKKGKGKNNGGLLPNSLRIISSCLKTVSTNASNVASTVRSAGASVAATISAASSEDQKDQ 60

Query: 61  VTWAGFDILEVEPYIIRHILLLGYLNGFQLLDVEDASNFKELVSKRGGPVSFLQILPSPA 120
           VTWAGFD LE+   + +H+LLLGY NGFQ+ DVEDASNF ELVSKR GPVSFLQ+ P PA
Sbjct: 61  VTWAGFDRLELPSSVFKHVLLLGYQNGFQVFDVEDASNFSELVSKRDGPVSFLQMQPFPA 120

Query: 121 KPGTPPAKSDRHELLRRSHPLLLIVAGEESKDVAMGQNHSPMGVLP---------GSCAN 180
                   SD +E  R SHPLLL+VAG++S   ++ QN S  G L          G+C +
Sbjct: 121 T-------SDGNEGYRASHPLLLVVAGDDSNSSSIVQNGSQSGGLGRNVNTESQLGNCVS 180

Query: 181 SHNAVQFYSLKSHSYVHVLRFRSAVCMVRCSSQIVAVGLATQIYCFDAITLEVVFSVLTS 240
           S   VQFYSL+SH YVHVLRFRSAVCMVRCS +IVAVGLATQIYCFDA+TLE  FSVL  
Sbjct: 181 SPTTVQFYSLRSHCYVHVLRFRSAVCMVRCSPRIVAVGLATQIYCFDAVTLENKFSVLIY 240

Query: 241 PVPEISGQGTTGTNVGYGPMAVGPRWLAYPSVGPVPSTTVPLSSQSPFPSQSVNPPSLPG 300
           PVP++SGQG+ G NVGYGPMAVGPRWLAY S  P+ + T  +S Q+  PS  V+P + PG
Sbjct: 241 PVPQLSGQGSIGVNVGYGPMAVGPRWLAYASNSPLVTNTGRVSPQNFSPSPGVSPSTSPG 300

Query: 301 SDRTKAHYPVK--------------------------------SGWKAGRLAGMEADYPG 360
                A Y ++                                SGWK GR AG E D  G
Sbjct: 301 GGSLVARYAMESSKQLAAVGYKTLSKYCQELLPDGSNSPVTSNSGWKIGRHAGTEMDNAG 360

Query: 361 MVAVKDFVTRAIITQFRAHTSPLSALCFDPSGTLLVTASIYGNNINIFRIIPN-SRSGSS 420
           MV VKDFV+RAII+QF+AHTSP+SALCFDPSGTLLVTASIYGNNINIFRI+P+ +R+GS 
Sbjct: 361 MVVVKDFVSRAIISQFKAHTSPISALCFDPSGTLLVTASIYGNNINIFRIMPSRTRNGSG 420

Query: 421 GLSFDCNSSHVHLYKLHRGITSAMIQDICFSNYSQWVAIVSSKGTCHVFLLSPFGGEAGL 480
               D ++SHVHLYKLHRGITSAMIQDICFSNYSQW+AIVSSKGTCH+F+LSPFGG+AG 
Sbjct: 421 VQGLDWSTSHVHLYKLHRGITSAMIQDICFSNYSQWIAIVSSKGTCHIFVLSPFGGDAGF 480

Query: 481 RILNSQGEEPCVLPVLTLPWWSTSSLIINQQSFPPPPPVTLSVVSRIKYSSFGWLNTVNN 540
           ++LNSQGEEP + P+L+LPWW TS  I N+QS PPPPPV LSVVSRIKYSSFGWL+TVN+
Sbjct: 481 QLLNSQGEEPALYPILSLPWWYTSLCITNRQSVPPPPPVALSVVSRIKYSSFGWLSTVNS 540

Query: 541 SAGA--GKGFVPSGAVAAIFHNTLSHNIQHVNSKPNSLEHLLVYTPSGHVVQHELLPSFG 600
           +A +  GK FVPSGAVAAIFHN+LS N+ H  S+ +SLEHLLVYTPSGH+VQHEL PS G
Sbjct: 541 TAASATGKVFVPSGAVAAIFHNSLSQNLPHGGSRVDSLEHLLVYTPSGHIVQHELQPSIG 600

Query: 601 AEPSLHSSRTESSSVLHMQEDDLKLKVEPIQWWDACRRSDYSERGECIHDSTSDGQDIAK 660
           AEPS   S T+S+S++HMQED+L++KVEPIQWWD CRRSD+ ER +C+  +T D +D+A 
Sbjct: 601 AEPSQGGSNTQSASLMHMQEDELRVKVEPIQWWDVCRRSDWPEREDCVLGTTFDKKDVA- 660

Query: 661 TNAIQNERTDTEKTHELDFQEMNDGSSADEILRVRGQSGITHEQSHWFLSNAEVQLSSGR 720
            + IQN ++  +  + L+F ++N G     +  V    G +HE+SHW+LSNAEVQ++S R
Sbjct: 661 -DKIQN-KSGPDNGYGLEFLDLNGGIGGRNL--VENYPGKSHERSHWYLSNAEVQINSLR 720

Query: 721 LPIWQNSKI--YVMSSPRINSIAGGEFEIEKVPVQEIEVREKELLPCFDHCLSLKSGCND 780
           LPIWQ SKI  YVM   R N +AGGEFE+EK+PV+EIE+R KELLP FDH  S K   N+
Sbjct: 721 LPIWQKSKICFYVMDCSRANGLAGGEFEVEKLPVREIEMRRKELLPVFDHVHSTKLIWNE 780

Query: 781 RGLLLGRCISPTSSEIYQSEVKVTEGIVVICHSKPASLSSTESSDGGSSRRMESSIDFDQ 832
           R +L GR  + T SE +  E K+ E   V+CHSKPASLSSTESSDGGSSRR+E+  DFDQ
Sbjct: 781 R-ILGGRYSNSTFSEPHHGEEKIMEE-TVMCHSKPASLSSTESSDGGSSRRIENLFDFDQ 840