Cucsa.084320 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.084320
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Kelch repeat-containing F-box family protein
Location	scaffold00862 : 79893 .. 80487 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTAGCCTGATTGAAGGTCTTCCTCATGACGTTGCCCTCAGATGCCTTGCTTTTGTTCCATTTTACCTCCACGCCACGTTGGAGCAAGTTTGCCATTCGTGGAGAGATGCCATTTGTAGtGGCGAAATTTATAAAGTTCGATCTGAGTGTGGTACGGCGGAGGATTTGCTATTCGTATGCTGTCATGATGAAGAAAaTAAATGGCAGTTTTATGAtCCTATTGAAAACTTTTGGGTCACCCTCCCTGAACTTCCAGGGGgAAGAAAGCATTATTTTGGTGTTGTCTCCACTCATCAAAAGCTGTTTATTCTTGGTGGTCTCTTGATAAATGCTATTGATCCATCAATCGATGAAGATTTTTCATGCAATGAGGTATGGTCCTTCAACCCTATGACTCGAAAATGGTCAATACAAGCACCAATGCACGAGGCACGTTCATTGTTTGCTTGTGGTATTTTGGATGGAATGATCATTGTTGTAGGTGGCATGAATAAAAAATTcGAATCAACTCCCAAGGCAGAAATGTATGACCCTGTGAAGGATGTTTGGATTCAATTGCCTGACCTGCCTCGAATCTGTGATTCGGGCATTT

mRNA sequence

ATGTCTAGCCTGATTGAAGGTCTTCCTCATGACGTTGCCCTCAGATGCCTTGCTTTTGTTCCATTTTACCTCCACGCCACGTTGGAGCAAGTTTGCCATTCGTGGAGAGATGCCATTTGTAGTGGCGAAATTTATAAAGTTCGATCTGAGTGTGGTACGGCGGAGGATTTGCTATTCGTATGCTGTCATGATGAAGAAAATAAATGGCAGTTTTATGATCCTATTGAAAACTTTTGGGTCACCCTCCCTGAACTTCCAGGGGGAAGAAAGCATTATTTTGGTGTTGTCTCCACTCATCAAAAGCTGTTTATTCTTGGTGGTCTCTTGATAAATGCTATTGATCCATCAATCGATGAAGATTTTTCATGCAATGAGGTATGGTCCTTCAACCCTATGACTCGAAAATGGTCAATACAAGCACCAATGCACGAGGCACGTTCATTGTTTGCTTGTGGTATTTTGGATGGAATGATCATTGTTGTAGGTGGCATGAATAAAAAATTCGAATCAACTCCCAAGGCAGAAATGTATGACCCTGTGAAGGATGTTTGGATTCAATTGCCTGACCTGCCTCGAATCTGTGATTCGGGCATTT

Coding sequence (CDS)

ATGTCTAGCCTGATTGAAGGTCTTCCTCATGACGTTGCCCTCAGATGCCTTGCTTTTGTTCCATTTTACCTCCACGCCACGTTGGAGCAAGTTTGCCATTCGTGGAGAGATGCCATTTGTAGtGGCGAAATTTATAAAGTTCGATCTGAGTGTGGTACGGCGGAGGATTTGCTATTCGTATGCTGTCATGATGAAGAAAaTAAATGGCAGTTTTATGAtCCTATTGAAAACTTTTGGGTCACCCTCCCTGAACTTCCAGGGGgAAGAAAGCATTATTTTGGTGTTGTCTCCACTCATCAAAAGCTGTTTATTCTTGGTGGTCTCTTGATAAATGCTATTGATCCATCAATCGATGAAGATTTTTCATGCAATGAGGTATGGTCCTTCAACCCTATGACTCGAAAATGGTCAATACAAGCACCAATGCACGAGGCACGTTCATTGTTTGCTTGTGGTATTTTGGATGGAATGATCATTGTTGTAGGTGGCATGAATAAAAAATTcGAATCAACTCCCAAGGCAGAAATGTATGACCCTGTGAAGGATGTTTGGATTCAATTGCCTGACCTGCCTCGAATCTGTGATTCGGGCATTT

Protein sequence

MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPVKDVWIQLPDLPRICDSGIX

Homology

[Close]

BLAST of Cucsa.084320 vs. Swiss-Prot
Match: SKI30_ARATH (F-box/kelch-repeat protein SKIP30 OS=Arabidopsis thaliana GN=SKIP30 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 9.9e-42
Identity = 89/194 (45.88%), Postives = 122/194 (62.89%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MS L++G+P  VALRCLA VP +LH  LE V  SWR AI S E+++VR E  ++E LL V
Sbjct: 8   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 67

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKH--YFGVVSTHQKLFILGG--LLINAIDPS 120
           C  D EN WQ Y P  + W+TLP LP   +H  +FG V+T   LF+LGG    ++ +   
Sbjct: 68  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 127

Query: 121 IDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEM 180
            D  F+ ++VWS++ + R+W+ +A M   R++FAC +L G I+V GG     +S   AEM
Sbjct: 128 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 187

Query: 181 YDPVKDVWIQLPDL 191
           YDP  DVW  +PDL
Sbjct: 188 YDPENDVWTSIPDL 201

[Close]

BLAST of Cucsa.084320 vs. Swiss-Prot
Match: FBK5_ARATH (F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana GN=At1g16250 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.9e-23
Identity = 64/196 (32.65%), Postives = 92/196 (46.94%), Query Frame = 1


Query: 3   SLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCC 62
           S+I GLP D+ALRC+A +    H  LE V   WRD +   +    ++  G +   LFV  
Sbjct: 7   SIIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVLT 66

Query: 63  HDEENKWQFYDPIENFWVTLPEL----PGGRKHYFGVVSTHQKLFILGGLLINAIDP-SI 122
              +N+W  YDP  + W  LP       G     F  V     L ++GG    ++     
Sbjct: 67  ERSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVCVSNCLLVIGGCYAPSVSSFPH 126

Query: 123 DEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMN-KKFESTPKAEM 182
            +     +V  F+P  ++W + A M   R+ FAC  + G + V GG N       P AE+
Sbjct: 127 QKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYVAGGRNLTHSRGIPSAEV 186

Query: 183 YDPVKDVWIQLPDLPR 193
           YDPV D W +LP +PR
Sbjct: 187 YDPVADRWEELPAMPR 202

[Close]

BLAST of Cucsa.084320 vs. Swiss-Prot
Match: FBK28_ARATH (F-box/kelch-repeat protein At1g67480 OS=Arabidopsis thaliana GN=At1g67480 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.7e-20
Identity = 65/192 (33.85%), Postives = 95/192 (49.48%), Query Frame = 1


Query: 4   LIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCCH 63
           LI GLP DVA +CLA VP     ++  VC  WR  + S E   VR   G  E+ L+V   
Sbjct: 39  LIPGLPDDVAKQCLALVPRARFPSMGSVCKKWRFVVQSKEFITVRRLAGMLEEWLYVLTM 98

Query: 64  D---EENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 123
           +   ++N+W+  D +     +LP +PG  K  F VV    KL ++ G  +  I+ S+   
Sbjct: 99  NAGGKDNRWEVMDCLGQKLSSLPPMPGPAKTGFKVVVVDGKLLVIAGCCM--INGSL--- 158

Query: 124 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 183
            +  +V+ ++     WS  A +  AR  FAC  ++G + VVGG     ES   AE+YDP 
Sbjct: 159 VASADVYQYDTCLNSWSRLADLEVARYDFACAEVNGHVYVVGGHGVDGESLSSAEVYDPE 218

Query: 184 KDVWIQLPDLPR 193
              W  +  L R
Sbjct: 219 TCTWTFIESLRR 225

[Close]

BLAST of Cucsa.084320 vs. Swiss-Prot
Match: FK132_ARATH (F-box/kelch-repeat protein At5g60570 OS=Arabidopsis thaliana GN=At5g60570 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.9e-16
Identity = 60/200 (30.00%), Postives = 91/200 (45.50%), Query Frame = 1


Query: 3   SLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCC 62
           S++ GL  DVAL CLA+VP   + +L  V   +   I SG ++ +R E G  E L+F+ C
Sbjct: 48  SVLPGLIDDVALNCLAWVPRSDYPSLSCVNKKYNKLINSGHLFALRKELGIVEYLVFMVC 107

Query: 63  HDEENKWQFYDPIENFWVTLPELPGGRKHYFG---VVSTHQKLFILGGLLINAIDPSIDE 122
             +   W  + P++  W+ LP++P            ++   +L + G  L          
Sbjct: 108 --DPRGWLMFSPMKKKWMVLPKMPCDECFNHADKESLAVDDELLVFGRELFQF------- 167

Query: 123 DFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDP 182
                 +W ++  +R W     MH  R LFA G L G+ IV GG +        AE+YD 
Sbjct: 168 -----AIWKYSLRSRCWVKCEGMHRPRCLFASGSLGGIAIVAGGTDMNGNILASAELYDS 227

Query: 183 VKDVWIQLPDL--PRICDSG 198
               W  LP++  PR   SG
Sbjct: 228 SSGRWEMLPNMHSPRRLCSG 233

[Close]

BLAST of Cucsa.084320 vs. Swiss-Prot
Match: AFR_ARATH (F-box protein AFR OS=Arabidopsis thaliana GN=AFR PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 7.2e-16
Identity = 58/201 (28.86%), Postives = 85/201 (42.29%), Query Frame = 1


Query: 4   LIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCCH 63
           LI GLP+D+A  CL  +P+  HA    V  SW   I +      +     +   LFV   
Sbjct: 28  LISGLPNDIAELCLLRLPYPYHALYRSVSSSWNKTITNPRFLFSKQSLSISSPYLFVFAF 87

Query: 64  DEEN---KWQFYDPIENFWVTLPELPG-----GRKHYFGVVSTHQ--KLFILGGLLINAI 123
           ++     +WQ  D     W  LP +P         H     S  +  KLF+LGG  +N  
Sbjct: 88  NKSTARIQWQSLDLASGRWFVLPPMPNSFTKISSPHALSCASMPRQGKLFVLGGGDVN-- 147

Query: 124 DPSIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGG-MNKKFESTP 183
                          +  +T +WS  +PM   R+ F  G ++G I+ VGG +    E+T 
Sbjct: 148 ----------RSAVVYTALTNRWSCISPMMSPRTYFVSGNVNGKIMAVGGSVGGNGEATT 207

Query: 184 KAEMYDPVKDVWIQLPDLPRI 194
           + E YDP  D W  +  LP +
Sbjct: 208 EVESYDPDNDTWTVVKKLPMV 216

[Close]

BLAST of Cucsa.084320 vs. TrEMBL
Match: A0A0A0K4Y2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G387790 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 5.5e-116
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV
Sbjct: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 120
           CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED
Sbjct: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 120

Query: 121 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 180
           FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV
Sbjct: 121 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 180

Query: 181 KDVWIQLPDLPRICDSGI 199
           KDVWIQLPDLPRICDSGI
Sbjct: 181 KDVWIQLPDLPRICDSGI 198

[Close]

BLAST of Cucsa.084320 vs. TrEMBL
Match: A0A0A0K7B1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G387780 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.1e-54
Identity = 111/201 (55.22%), Postives = 139/201 (69.15%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MS LIEGLP  ++LRCLA++P+YLH  LE V  SW+ AI S E+++ R E G +ED L V
Sbjct: 1   MSGLIEGLPDAISLRCLAYIPYYLHPKLELVSRSWKAAIRSVELFRARQEVGFSEDFLCV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHY--FGVVSTHQKLFILGGLLINAIDP--- 120
           C +   N WQ YDP+ N W+TLPELP  R H   F  VST QKLF+LGG   +A+DP   
Sbjct: 61  CSYHPNNTWQLYDPLPNRWMTLPELPSKRMHLGNFCAVSTSQKLFVLGG-RSDAVDPVTG 120

Query: 121 SIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAE 180
             D++FS NEVWSF+P+TR WS++APM   R++FAC ++DG IIV GG   K +ST KAE
Sbjct: 121 DRDDNFSTNEVWSFDPITRTWSMRAPMLVPRAMFACCVVDGKIIVAGGFTSKSKSTSKAE 180

Query: 181 MYDPVKDVWIQLPDLPRICDS 197
           MYD  KDVW  LPDL +  DS
Sbjct: 181 MYDSEKDVWTPLPDLLQTHDS 200

[Close]

BLAST of Cucsa.084320 vs. TrEMBL
Match: B9SFJ2_RICCO (Protein AFR, putative OS=Ricinus communis GN=RCOM_0646580 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-52
Identity = 107/201 (53.23%), Postives = 140/201 (69.65%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MS LIEGLP  +A+RC+A VPFYLH  LE V HSWR AI S E++K R E G+AEDLL V
Sbjct: 1   MSELIEGLPDAIAIRCIARVPFYLHPKLELVSHSWRSAIRSPELFKARQEVGSAEDLLCV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKH--YFGVVSTHQKLFILGGLLINAIDP--- 120
           C  + EN WQ YDP+ + W+TLP LP   +H  +FGVVST  KL++LGG   +A+DP   
Sbjct: 61  CAFEPENLWQLYDPLRDLWITLPVLPSKIRHLAHFGVVSTAGKLYVLGG-GSDAVDPLTG 120

Query: 121 SIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAE 180
             D +F+ NEVWS++P+ R+W+++A M   R++FAC +L G I+V GG     +S  +AE
Sbjct: 121 DQDGNFATNEVWSYDPVIRQWALRASMLVPRAMFACCVLKGKIVVAGGFTSCRKSISQAE 180

Query: 181 MYDPVKDVWIQLPDLPRICDS 197
           MYDP KDVWI +PDL R  +S
Sbjct: 181 MYDPEKDVWIPIPDLHRTHNS 200

[Close]

BLAST of Cucsa.084320 vs. TrEMBL
Match: F6HI50_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0151g00770 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 4.3e-52
Identity = 107/201 (53.23%), Postives = 138/201 (68.66%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MS LIEGLP  VALRCLA+VPFYLH  LE V  SWRDAI   E++K R E G++EDLL V
Sbjct: 1   MSGLIEGLPDAVALRCLAWVPFYLHPRLELVSRSWRDAIRGPELFKARQEVGSSEDLLCV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKH--YFGVVSTHQKLFILGGLLINAIDP--- 120
           C  D EN WQ YDP ++ W++LP LP   +H  +FG VST  KLF+LGG   +A+DP   
Sbjct: 61  CAFDPENLWQLYDPRKDLWISLPVLPSRIRHLAHFGAVSTAGKLFVLGG-GSDAVDPLTG 120

Query: 121 SIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAE 180
             D  F+ NEVWS++P+ R+W+ +APM   R++FAC +LDG I+V GG     +S  +AE
Sbjct: 121 DQDGSFATNEVWSYDPIIRQWAPRAPMLVPRAMFACCVLDGKIVVAGGFTSCRKSISQAE 180

Query: 181 MYDPVKDVWIQLPDLPRICDS 197
           +YDP KD W+ +PDL R  +S
Sbjct: 181 IYDPEKDAWVSIPDLHRTHNS 200

[Close]

BLAST of Cucsa.084320 vs. TrEMBL
Match: A0A059AZG4_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H01462 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 5.7e-52
Identity = 108/201 (53.73%), Postives = 138/201 (68.66%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MS LIEGLP  VALRCLA VPF+LH TLE V  SW+ AI S E++K R E G+AEDLL V
Sbjct: 1   MSGLIEGLPDAVALRCLARVPFHLHPTLELVSRSWKAAIRSPELFKARQEVGSAEDLLCV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKH--YFGVVSTHQKLFILGGLLINAIDPSI- 120
           C  D EN WQ YDP+ + W+TLP LP   +H  +FG VST  KLF++GG   +A+DP   
Sbjct: 61  CAFDPENLWQLYDPLRDLWITLPVLPSKIRHLSHFGTVSTAGKLFVIGG-GSDAVDPQTG 120

Query: 121 --DEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAE 180
             D  F+ NEVWS++P+TR+W+  A M   R++FAC  L+G I+V GG     +S  +AE
Sbjct: 121 DQDGSFATNEVWSYDPVTRQWASHASMLVPRAMFACCALNGKIVVAGGFTSCRKSISQAE 180

Query: 181 MYDPVKDVWIQLPDLPRICDS 197
           MYDP KD+W+Q+PDL R  +S
Sbjct: 181 MYDPDKDLWVQIPDLHRTHNS 200

[Close]

BLAST of Cucsa.084320 vs. TAIR10
Match: AT3G63220.2 (AT3G63220.2 Galactose oxidase/kelch repeat superfamily protein)

HSP 1 Score: 171.4 bits (433), Expect = 5.6e-43
Identity = 89/194 (45.88%), Postives = 122/194 (62.89%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MS L++G+P  VALRCLA VP +LH  LE V  SWR AI S E+++VR E  ++E LL V
Sbjct: 8   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 67

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKH--YFGVVSTHQKLFILGG--LLINAIDPS 120
           C  D EN WQ Y P  + W+TLP LP   +H  +FG V+T   LF+LGG    ++ +   
Sbjct: 68  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 127

Query: 121 IDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEM 180
            D  F+ ++VWS++ + R+W+ +A M   R++FAC +L G I+V GG     +S   AEM
Sbjct: 128 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 187

Query: 181 YDPVKDVWIQLPDL 191
           YDP  DVW  +PDL
Sbjct: 188 YDPENDVWTSIPDL 201

[Close]

BLAST of Cucsa.084320 vs. TAIR10
Match: AT1G16250.1 (AT1G16250.1 Galactose oxidase/kelch repeat superfamily protein)

HSP 1 Score: 108.6 bits (270), Expect = 4.5e-24
Identity = 64/196 (32.65%), Postives = 92/196 (46.94%), Query Frame = 1


Query: 3   SLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCC 62
           S+I GLP D+ALRC+A +    H  LE V   WRD +   +    ++  G +   LFV  
Sbjct: 7   SIIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVLT 66

Query: 63  HDEENKWQFYDPIENFWVTLPEL----PGGRKHYFGVVSTHQKLFILGGLLINAIDP-SI 122
              +N+W  YDP  + W  LP       G     F  V     L ++GG    ++     
Sbjct: 67  ERSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVCVSNCLLVIGGCYAPSVSSFPH 126

Query: 123 DEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMN-KKFESTPKAEM 182
            +     +V  F+P  ++W + A M   R+ FAC  + G + V GG N       P AE+
Sbjct: 127 QKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYVAGGRNLTHSRGIPSAEV 186

Query: 183 YDPVKDVWIQLPDLPR 193
           YDPV D W +LP +PR
Sbjct: 187 YDPVADRWEELPAMPR 202

[Close]

BLAST of Cucsa.084320 vs. TAIR10
Match: AT1G67480.1 (AT1G67480.1 Galactose oxidase/kelch repeat superfamily protein)

HSP 1 Score: 99.8 bits (247), Expect = 2.1e-21
Identity = 65/192 (33.85%), Postives = 95/192 (49.48%), Query Frame = 1


Query: 4   LIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCCH 63
           LI GLP DVA +CLA VP     ++  VC  WR  + S E   VR   G  E+ L+V   
Sbjct: 39  LIPGLPDDVAKQCLALVPRARFPSMGSVCKKWRFVVQSKEFITVRRLAGMLEEWLYVLTM 98

Query: 64  D---EENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 123
           +   ++N+W+  D +     +LP +PG  K  F VV    KL ++ G  +  I+ S+   
Sbjct: 99  NAGGKDNRWEVMDCLGQKLSSLPPMPGPAKTGFKVVVVDGKLLVIAGCCM--INGSL--- 158

Query: 124 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 183
            +  +V+ ++     WS  A +  AR  FAC  ++G + VVGG     ES   AE+YDP 
Sbjct: 159 VASADVYQYDTCLNSWSRLADLEVARYDFACAEVNGHVYVVGGHGVDGESLSSAEVYDPE 218

Query: 184 KDVWIQLPDLPR 193
              W  +  L R
Sbjct: 219 TCTWTFIESLRR 225

[Close]

BLAST of Cucsa.084320 vs. TAIR10
Match: AT5G60570.1 (AT5G60570.1 Galactose oxidase/kelch repeat superfamily protein)

HSP 1 Score: 87.4 bits (215), Expect = 1.1e-17
Identity = 60/200 (30.00%), Postives = 91/200 (45.50%), Query Frame = 1


Query: 3   SLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCC 62
           S++ GL  DVAL CLA+VP   + +L  V   +   I SG ++ +R E G  E L+F+ C
Sbjct: 48  SVLPGLIDDVALNCLAWVPRSDYPSLSCVNKKYNKLINSGHLFALRKELGIVEYLVFMVC 107

Query: 63  HDEENKWQFYDPIENFWVTLPELPGGRKHYFG---VVSTHQKLFILGGLLINAIDPSIDE 122
             +   W  + P++  W+ LP++P            ++   +L + G  L          
Sbjct: 108 --DPRGWLMFSPMKKKWMVLPKMPCDECFNHADKESLAVDDELLVFGRELFQF------- 167

Query: 123 DFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDP 182
                 +W ++  +R W     MH  R LFA G L G+ IV GG +        AE+YD 
Sbjct: 168 -----AIWKYSLRSRCWVKCEGMHRPRCLFASGSLGGIAIVAGGTDMNGNILASAELYDS 227

Query: 183 VKDVWIQLPDL--PRICDSG 198
               W  LP++  PR   SG
Sbjct: 228 SSGRWEMLPNMHSPRRLCSG 233

[Close]

BLAST of Cucsa.084320 vs. TAIR10
Match: AT2G24540.1 (AT2G24540.1 Galactose oxidase/kelch repeat superfamily protein)

HSP 1 Score: 85.5 bits (210), Expect = 4.0e-17
Identity = 58/201 (28.86%), Postives = 85/201 (42.29%), Query Frame = 1


Query: 4   LIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFVCCH 63
           LI GLP+D+A  CL  +P+  HA    V  SW   I +      +     +   LFV   
Sbjct: 28  LISGLPNDIAELCLLRLPYPYHALYRSVSSSWNKTITNPRFLFSKQSLSISSPYLFVFAF 87

Query: 64  DEEN---KWQFYDPIENFWVTLPELPG-----GRKHYFGVVSTHQ--KLFILGGLLINAI 123
           ++     +WQ  D     W  LP +P         H     S  +  KLF+LGG  +N  
Sbjct: 88  NKSTARIQWQSLDLASGRWFVLPPMPNSFTKISSPHALSCASMPRQGKLFVLGGGDVN-- 147

Query: 124 DPSIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGG-MNKKFESTP 183
                          +  +T +WS  +PM   R+ F  G ++G I+ VGG +    E+T 
Sbjct: 148 ----------RSAVVYTALTNRWSCISPMMSPRTYFVSGNVNGKIMAVGGSVGGNGEATT 207

Query: 184 KAEMYDPVKDVWIQLPDLPRI 194
           + E YDP  D W  +  LP +
Sbjct: 208 EVESYDPDNDTWTVVKKLPMV 216

[Close]

BLAST of Cucsa.084320 vs. NCBI nr
Match: gi|700189562|gb|KGN44795.1| (hypothetical protein Csa_7G387790 [Cucumis sativus])

HSP 1 Score: 424.9 bits (1091), Expect = 8.0e-116
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV
Sbjct: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 120
           CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED
Sbjct: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 120

Query: 121 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 180
           FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV
Sbjct: 121 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 180

Query: 181 KDVWIQLPDLPRICDSGI 199
           KDVWIQLPDLPRICDSGI
Sbjct: 181 KDVWIQLPDLPRICDSGI 198

[Close]

BLAST of Cucsa.084320 vs. NCBI nr
Match: gi|778730390|ref|XP_011659773.1| (PREDICTED: F-box/kelch-repeat protein SKIP30-like [Cucumis sativus])

HSP 1 Score: 424.9 bits (1091), Expect = 8.0e-116
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV
Sbjct: 40  MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 99

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 120
           CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED
Sbjct: 100 CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 159

Query: 121 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 180
           FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV
Sbjct: 160 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 219

Query: 181 KDVWIQLPDLPRICDSGI 199
           KDVWIQLPDLPRICDSGI
Sbjct: 220 KDVWIQLPDLPRICDSGI 237

[Close]

BLAST of Cucsa.084320 vs. NCBI nr
Match: gi|659100900|ref|XP_008451325.1| (PREDICTED: F-box/kelch-repeat protein SKIP30-like [Cucumis melo])

HSP 1 Score: 401.7 bits (1031), Expect = 7.2e-109
Identity = 186/198 (93.94%), Postives = 190/198 (95.96%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MSSLIEGLP DVALRCLAFVPFYLH TLEQVC SWRDAICSGEIY+VR ECG AEDLLFV
Sbjct: 1   MSSLIEGLPRDVALRCLAFVPFYLHPTLEQVCRSWRDAICSGEIYRVRHECGKAEDLLFV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGGLLINAIDPSIDED 120
           CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVST+QKLFILGGLLIN IDPS DED
Sbjct: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTYQKLFILGGLLINPIDPSADED 120

Query: 121 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAEMYDPV 180
           FSCNEVWSFNPMTRKWSIQAPMHEARSLFACG+LDGMIIVVGGMNKKFE+TPKAEMYDPV
Sbjct: 121 FSCNEVWSFNPMTRKWSIQAPMHEARSLFACGVLDGMIIVVGGMNKKFEATPKAEMYDPV 180

Query: 181 KDVWIQLPDLPRICDSGI 199
           KDVW QLPDLPRICDSGI
Sbjct: 181 KDVWTQLPDLPRICDSGI 198

[Close]

BLAST of Cucsa.084320 vs. NCBI nr
Match: gi|449458480|ref|XP_004146975.1| (PREDICTED: F-box/kelch-repeat protein SKIP30-like [Cucumis sativus])

HSP 1 Score: 220.3 bits (560), Expect = 3.0e-54
Identity = 111/201 (55.22%), Postives = 139/201 (69.15%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MS LIEGLP  ++LRCLA++P+YLH  LE V  SW+ AI S E+++ R E G +ED L V
Sbjct: 1   MSGLIEGLPDAISLRCLAYIPYYLHPKLELVSRSWKAAIRSVELFRARQEVGFSEDFLCV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHY--FGVVSTHQKLFILGGLLINAIDP--- 120
           C +   N WQ YDP+ N W+TLPELP  R H   F  VST QKLF+LGG   +A+DP   
Sbjct: 61  CSYHPNNTWQLYDPLPNRWMTLPELPSKRMHLGNFCAVSTSQKLFVLGG-RSDAVDPVTG 120

Query: 121 SIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAE 180
             D++FS NEVWSF+P+TR WS++APM   R++FAC ++DG IIV GG   K +ST KAE
Sbjct: 121 DRDDNFSTNEVWSFDPITRTWSMRAPMLVPRAMFACCVVDGKIIVAGGFTSKSKSTSKAE 180

Query: 181 MYDPVKDVWIQLPDLPRICDS 197
           MYD  KDVW  LPDL +  DS
Sbjct: 181 MYDSEKDVWTPLPDLLQTHDS 200

[Close]

BLAST of Cucsa.084320 vs. NCBI nr
Match: gi|659100898|ref|XP_008451324.1| (PREDICTED: F-box/kelch-repeat protein SKIP30-like [Cucumis melo])

HSP 1 Score: 219.5 bits (558), Expect = 5.1e-54
Identity = 111/201 (55.22%), Postives = 138/201 (68.66%), Query Frame = 1


Query: 1   MSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGTAEDLLFV 60
           MS LIEGLP  ++LRCLA++P+YLH  L  V  SW+ AI S E+++ R E G +ED L V
Sbjct: 1   MSGLIEGLPDAISLRCLAYIPYYLHPKLALVSRSWKAAIRSVELFRARQEVGFSEDFLCV 60

Query: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHY--FGVVSTHQKLFILGGLLINAIDP--- 120
           C +   N WQ YDP+ N W+TLPELP  R H   F  VST QKLF+LGG  I A+DP   
Sbjct: 61  CSYHPNNTWQLYDPLPNHWMTLPELPSKRMHLGNFCTVSTSQKLFVLGGRSI-AVDPVTG 120

Query: 121 SIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFESTPKAE 180
             D++FS NEVWSF+P+TR WS++APM   R++FAC ++DG IIV GG   K +ST KAE
Sbjct: 121 DRDDNFSTNEVWSFDPITRMWSMRAPMLVPRAMFACCVVDGKIIVAGGFTSKSKSTSKAE 180

Query: 181 MYDPVKDVWIQLPDLPRICDS 197
           MYD  KDVW  LPDL +  DS
Sbjct: 181 MYDSEKDVWTLLPDLLQTHDS 200

The following BLAST results are available for this feature:

BLAST of Cucsa.084320 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SKI30_ARATH	9.9e-42	45.88	F-box/kelch-repeat protein SKIP30 OS=Arabidopsis thaliana GN=SKIP30 PE=1 SV=2	[more]
FBK5_ARATH	7.9e-23	32.65	F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana GN=At1g16250 PE=2 S...	[more]
FBK28_ARATH	3.7e-20	33.85	F-box/kelch-repeat protein At1g67480 OS=Arabidopsis thaliana GN=At1g67480 PE=2 S...	[more]
FK132_ARATH	1.9e-16	30.00	F-box/kelch-repeat protein At5g60570 OS=Arabidopsis thaliana GN=At5g60570 PE=2 S...	[more]
AFR_ARATH	7.2e-16	28.86	F-box protein AFR OS=Arabidopsis thaliana GN=AFR PE=1 SV=2	[more]

BLAST of Cucsa.084320 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4Y2_CUCSA	5.5e-116	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G387790 PE=4 SV=1	[more]
A0A0A0K7B1_CUCSA	2.1e-54	55.22	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G387780 PE=4 SV=1	[more]
B9SFJ2_RICCO	1.5e-52	53.23	Protein AFR, putative OS=Ricinus communis GN=RCOM_0646580 PE=4 SV=1	[more]
F6HI50_VITVI	4.3e-52	53.23	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0151g00770 PE=4 SV=...	[more]
A0A059AZG4_EUCGR	5.7e-52	53.73	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H01462 PE=4 SV=1	[more]

BLAST of Cucsa.084320 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G63220.2	5.6e-43	45.88	Galactose oxidase/kelch repeat superfamily protein	[more]
AT1G16250.1	4.5e-24	32.65	Galactose oxidase/kelch repeat superfamily protein	[more]
AT1G67480.1	2.1e-21	33.85	Galactose oxidase/kelch repeat superfamily protein	[more]
AT5G60570.1	1.1e-17	30.00	Galactose oxidase/kelch repeat superfamily protein	[more]
AT2G24540.1	4.0e-17	28.86	Galactose oxidase/kelch repeat superfamily protein	[more]

BLAST of Cucsa.084320 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700189562\|gb\|KGN44795.1\|	8.0e-116	100.00	hypothetical protein Csa_7G387790 [Cucumis sativus]	[more]
gi\|778730390\|ref\|XP_011659773.1\|	8.0e-116	100.00	PREDICTED: F-box/kelch-repeat protein SKIP30-like [Cucumis sativus]	[more]
gi\|659100900\|ref\|XP_008451325.1\|	7.2e-109	93.94	PREDICTED: F-box/kelch-repeat protein SKIP30-like [Cucumis melo]	[more]
gi\|449458480\|ref\|XP_004146975.1\|	3.0e-54	55.22	PREDICTED: F-box/kelch-repeat protein SKIP30-like [Cucumis sativus]	[more]
gi\|659100898\|ref\|XP_008451324.1\|	5.1e-54	55.22	PREDICTED: F-box/kelch-repeat protein SKIP30-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001810	F-box_dom
IPR006652	Kelch_1
IPR015916	Galactose oxidase, beta-propeller

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.084320.1	Cucsa.084320.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001810	F-box domain	SMART	SM00256	fbox_2	coord: 8..48 score: 0.
IPR001810	F-box domain	unknown	SSF81383	F-box domain	coord: 4..46 score: 9.6
IPR006652	Kelch repeat type 1	PFAM	PF01344	Kelch_1	coord: 145..191 score: 4.
IPR006652	Kelch repeat type 1	SMART	SM00612	kelc_smart	coord: 101..156 score: 0.023coord: 157..196 score:
IPR015916	Galactose oxidase, beta-propeller	GENE3D	G3DSA:2.130.10.80		coord: 38..192 score: 4.4
None	No IPR available	GENE3D	G3DSA:1.20.1280.50		coord: 4..37 score: 4.
None	No IPR available	PANTHER	PTHR24414	FAMILY NOT NAMED	coord: 2..193 score: 2.7
None	No IPR available	unknown	SSF117281	Kelch motif	coord: 61..194 score: 1.83

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.084320	Bottle gourd (USVL1VR-Ls)	cgylsiB090
Cucsa.084320	Melon (DHL92) v3.6.1	cgymedB091
Cucsa.084320	Silver-seed gourd	carcgyB0063
Cucsa.084320	Silver-seed gourd	carcgyB0129
Cucsa.084320	Silver-seed gourd	carcgyB0842
Cucsa.084320	Cucumber (Chinese Long) v3	cgycucB087
Cucsa.084320	Watermelon (97103) v2	cgywmbB089
Cucsa.084320	Wax gourd	cgywgoB104
Cucsa.084320	Wax gourd	cgywgoB105
Cucsa.084320	Cucurbita maxima (Rimu)	cgycmaB0157
Cucsa.084320	Cucurbita maxima (Rimu)	cgycmaB0158
Cucsa.084320	Cucurbita moschata (Rifu)	cgycmoB0152
Cucsa.084320	Cucurbita moschata (Rifu)	cgycmoB0153
Cucsa.084320	Wild cucumber (PI 183967)	cgycpiB088
Cucsa.084320	Cucumber (Chinese Long) v2	cgycuB085
Cucsa.084320	Melon (DHL92) v3.5.1	cgymeB093
Cucsa.084320	Watermelon (Charleston Gray)	cgywcgB091
Cucsa.084320	Watermelon (97103) v1	cgywmB094
Cucsa.084320	Cucurbita pepo (Zucchini)	cgycpeB0151
Cucsa.084320	Cucurbita pepo (Zucchini)	cgycpeB0153