BLAST of Cucsa.073050 vs. Swiss-Prot
Match:
C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 501.9 bits (1291), Expect = 8.1e-141
Identity = 256/489 (52.35%), Postives = 341/489 (69.73%), Query Frame = 1
Query: 12 FLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYGHVFSLRFG 71
F+I++ + LK+ KNLPP P ++P+IG+LH LKHP+H+T +LS+ YG +FSL FG
Sbjct: 12 FIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFG 71
Query: 72 SYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWRRLRRIST 131
S LVVV+SSPS ECFTKNDIILANRP TGK++ YN+T L A YG+ WR LRRI+T
Sbjct: 72 SRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 131
Query: 132 CEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFTSNIVMRMV 191
++ S ++LNSF +R+DE RL+ KL ++ V E F+ VEL P L + T N +MRM+
Sbjct: 132 IDVLSNNRLNSFLGVRRDETNRLIQKLL-KDVVSEGFGFTKVELRPRLTEMTFNAMMRMI 191
Query: 192 GGKRYFGDD--VLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKIMKVG 251
GKRY+GDD V D +A++FR+++ +M GA N GDF+PL +D LEK+ ++
Sbjct: 192 SGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIA 251
Query: 252 EEADEIFQGLIDEIR--NEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVILLAGIDT 311
+ ++ +GLI+E R N DG TMI HLL L +QPEY+SD +IKGLI +LLAG DT
Sbjct: 252 KRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDT 311
Query: 312 ISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETLRLTPA 371
+VT+EW +S LLN+P+V+KKA+ E++ +G+ +LV+E DLS L YLQ II ETLRL P
Sbjct: 312 SAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPP 371
Query: 372 APLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHENAIELSDS 431
APLL+PH +SEDC I +++P+DTII N W I RD W D SFKPER E E++
Sbjct: 372 APLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEEEVN-- 431
Query: 432 YKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPKAQPL 491
K++ FGLGRRACPG+ +AQR +G T+ LIQCF+WER +DM EG+GITMP PL
Sbjct: 432 -KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPL 491
Query: 492 VAKCKPRPI 497
A CK PI
Sbjct: 492 RAMCKALPI 495
BLAST of Cucsa.073050 vs. Swiss-Prot
Match:
C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 497.7 bits (1280), Expect = 1.5e-139
Identity = 261/504 (51.79%), Postives = 345/504 (68.45%), Query Frame = 1
Query: 5 ILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYGH 64
+++Y F LI L++ K L +NLPP+P LP+IGHL LLK PIH+TL S S+ H
Sbjct: 8 VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67
Query: 65 -----VFSLRFGSYLVVVISSPS-AVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAP 124
V SLR GS LV V+SS A ECF KND++LANRP + GKH+ YN T + AP
Sbjct: 68 NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127
Query: 125 YGELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPI 184
YG+ WR LRR+ T EIFST +LN F +R DEV+RL+ +L ++ + VEL+P+
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK----TVVELKPM 187
Query: 185 LLDFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDP 244
L+D T N +MRM+ GKRY+G++ DE +A++ R +V V + N D++P+
Sbjct: 188 LMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF-- 247
Query: 245 TGLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIH 304
+ E ++ K+GEE D+ QGLID+ R ++E G TMI HLL LQ + EY++DQIIKG+I
Sbjct: 248 SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIIL 307
Query: 305 VILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGII 364
++++AG +T +VTLEW LS+LLN+P VI KAR EI++ VG +RL+ E DLS L YL+ I+
Sbjct: 308 IMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIV 367
Query: 365 LETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERH 424
LETLRL PA PLLVPH ASEDC+I YD+PR T + VNAWAI RD + W+D SFKPER
Sbjct: 368 LETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF 427
Query: 425 ENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQG 484
E ++ KLL FGLGRRACPG G+AQR++GL L SLIQCF+WER+ + VDM EG G
Sbjct: 428 EKE---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVG 487
Query: 485 ITMPKAQPLVAKCKPRPIMRAHLS 503
T+PKA PL A CK RP + +S
Sbjct: 488 NTVPKAIPLKAICKARPFLHKIIS 502
BLAST of Cucsa.073050 vs. Swiss-Prot
Match:
C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 496.9 bits (1278), Expect = 2.6e-139
Identity = 257/493 (52.13%), Postives = 341/493 (69.17%), Query Frame = 1
Query: 4 NILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYG 63
++++ +FF +I+L + KNLPP P LP+IG+LH LK P+H T H+LS+KYG
Sbjct: 7 SLIISLFFLIITLKVFFNTSRKF-KNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQKYG 66
Query: 64 HVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELW 123
+FSL FGS LVVV+SS + +ECFTKNDI+LANRP TGK++ YN T +A +PYG+ W
Sbjct: 67 QIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHW 126
Query: 124 RRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFT 183
R LRRI + EI S+ +LNSF IR+DE+ RL+ KL ++ F+ VEL P+ + T
Sbjct: 127 RNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNG----FTEVELRPMFSEMT 186
Query: 184 SNIVMRMVGGKRYFGDD--VLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGL 243
N +MRMV GKRY+G+D V D +A FR ++K V+ GA N GDF+ W D GL
Sbjct: 187 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL 246
Query: 244 EKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVIL 303
EK++ K+ + D QGLIDE R + + NTMI HLL Q +QPEY++DQIIKGL+ V+L
Sbjct: 247 EKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVML 306
Query: 304 LAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILET 363
LAG DT SVT+EW +S+LLN+P+++KKA+ E++ +G +R V+E D+S L YLQ I+ ET
Sbjct: 307 LAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYET 366
Query: 364 LRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHENA 423
LRL AAPLLVPH +SED + GY+IP++TI+ VNAW I RD +LW D T FKPER E
Sbjct: 367 LRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKE 426
Query: 424 IELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITM 483
E++ KLL FGLGRRACPG ++QR GLTL LIQCF+W+R+ +DM E +GIT
Sbjct: 427 GEVN---KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITA 486
Query: 484 PKAQPLVAKCKPR 495
K L A CK R
Sbjct: 487 GKKTSLNAMCKVR 490
BLAST of Cucsa.073050 vs. Swiss-Prot
Match:
C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 491.9 bits (1265), Expect = 8.4e-138
Identity = 244/491 (49.69%), Postives = 341/491 (69.45%), Query Frame = 1
Query: 9 IFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYGHVFSL 68
+F+ + + + + KNLPP P SLP+IG+LH LK P+H+T LS+KYGHVFSL
Sbjct: 11 VFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 70
Query: 69 RFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWRRLRR 128
FGS LVVV+SS S ++CFTKND++LANRP +GK++ YN+T L YGE WR LRR
Sbjct: 71 WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 130
Query: 129 ISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFTSNIVM 188
I+ ++ S ++NSFS IR+DE +RL+ +L + +S F+ +EL L D T N +M
Sbjct: 131 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTN----FAEMELSSRLYDMTFNNIM 190
Query: 189 RMVGGKRYFGDD--VLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKIM 248
RM+ GKRY+G+D D +A +FRD+V ++ +GA N DF+PL ++D LEK++
Sbjct: 191 RMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLK 250
Query: 249 KVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVILLAGID 308
+ + D +GLI+E R ++E NTMI HLL+LQ++QPEY++DQIIKGL +LLAG D
Sbjct: 251 DISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTD 310
Query: 309 TISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETLRLTP 368
+ +VTLEW +S+LLN+P+V+KK + E++ VGQ+RLV+E DL L+YL+ +I ETLRL
Sbjct: 311 SSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYT 370
Query: 369 AAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHENAIELSD 428
APLL+PH S++C I GY +P+DTI+ +NAWAI RD LW + T+FKPER E EL
Sbjct: 371 PAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE- 430
Query: 429 SYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPKAQP 488
KL+ FG+GRRACPG G+A R + +TLA LIQCFDW+ ++ +D+ E G T+ K P
Sbjct: 431 --KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVP 490
Query: 489 LVAKCKPRPIM 498
L A CK RP++
Sbjct: 491 LKAMCKSRPVI 493
BLAST of Cucsa.073050 vs. Swiss-Prot
Match:
C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 483.4 bits (1243), Expect = 3.0e-135
Identity = 243/499 (48.70%), Postives = 348/499 (69.74%), Query Frame = 1
Query: 5 ILVYIFF--FLISLLLGLKLLLHHGK--NLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 64
IL Y+ + F +S+ ++LL K NLPP P SLP+IG+LH LK P+++T +L++
Sbjct: 3 ILSYLCYSLFYLSIFFIIRLLFQSRKFKNLPPGPT-SLPIIGNLHHLKRPLNRTFKALTE 62
Query: 65 KYGHVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 124
KYG+V SL FGS LVVV+SS S +ECFTKND++LANRP +GK++ YN+T L YG
Sbjct: 63 KYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 122
Query: 125 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL 184
E WR LRRI++ ++ S ++N+F+ IR+DE +RL+ KL +S + F+ VEL
Sbjct: 123 EHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTK----FAEVELTFRFF 182
Query: 185 DFTSNIVMRMVGGKRYFGDD--VLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDP 244
D T N +MRM+ GKRY+GDD + + +A +FRD+V ++ +GA N DF+PL ++D
Sbjct: 183 DMTFNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDF 242
Query: 245 TGLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIH 304
LEK++ ++GE+ D GL+ E R+++E NTMI HLL++Q +QPEY++D IIKGL
Sbjct: 243 ENLEKRVKRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCL 302
Query: 305 VILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGII 364
+LLAG D+ +VTLEW +S++LN P+V+KK R E++ VGQ+RLV+E DL L+YL+ +I
Sbjct: 303 AMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVI 362
Query: 365 LETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERH 424
ETLRL APLL+PH +++C + GY +PRDTI+ +NAWAI RD W + T+FKPER
Sbjct: 363 YETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF 422
Query: 425 ENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQG 484
+ EL K++ FG+GRRACPG G+A R + +TLA L+QCFDW+R++ +DM+E G
Sbjct: 423 DKKGELE---KMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDG 482
Query: 485 ITMPKAQPLVAKCKPRPIM 498
TM K PL A CK RP++
Sbjct: 483 FTMTKLLPLKAMCKTRPVV 493
BLAST of Cucsa.073050 vs. TrEMBL
Match:
A0A0A0LTD0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425740 PE=3 SV=1)
HSP 1 Score: 1004.2 bits (2595), Expect = 5.6e-290
Identity = 498/502 (99.20%), Postives = 499/502 (99.40%), Query Frame = 1
Query: 1 MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 60
MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK
Sbjct: 1 MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 60
Query: 61 KYGHVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 120
KYGHVFSLRFGS+LVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG
Sbjct: 61 KYGHVFSLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 120
Query: 121 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL 180
ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL
Sbjct: 121 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL 180
Query: 181 DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG 240
DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG
Sbjct: 181 DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG 240
Query: 241 LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI 300
LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI
Sbjct: 241 LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI 300
Query: 301 LLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE 360
LAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE
Sbjct: 301 FLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE 360
Query: 361 TLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHEN 420
TLRLTPAAPLLVPHCASEDCQIEGYDIPR TIIFVNAWAI RDSSLWEDVTSFKPERHEN
Sbjct: 361 TLRLTPAAPLLVPHCASEDCQIEGYDIPRGTIIFVNAWAIHRDSSLWEDVTSFKPERHEN 420
Query: 421 AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT 480
AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT
Sbjct: 421 AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT 480
Query: 481 MPKAQPLVAKCKPRPIMRAHLS 503
MPKAQPLVAKCKPRPIMRAHLS
Sbjct: 481 MPKAQPLVAKCKPRPIMRAHLS 502
BLAST of Cucsa.073050 vs. TrEMBL
Match:
A0A0A0LQI5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423640 PE=3 SV=1)
HSP 1 Score: 689.5 bits (1778), Expect = 3.1e-195
Identity = 338/508 (66.54%), Postives = 411/508 (80.91%), Query Frame = 1
Query: 1 MDNNILVYIFFFLISLLLGLKLLLH-HGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLS 60
MD + L+Y LISLLLG L H NLPPTPLF LP IGHLHLLKHP+H+TL +LS
Sbjct: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
Query: 61 KKYGHVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPY 120
+KYGHVFSL+FGS LV+++SSPSAV+ECFTKNDIILANRP LN+GK+LSYN T +AV+PY
Sbjct: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
Query: 121 GELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPIL 180
GE WR LRRI T EIFST++LNSFS IR++EVKRLL KLC E+ F VELE +L
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDE--FRVVELESML 180
Query: 181 LDFTSNIVMRMVGGKRYFGDD------VLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLW 240
LD TSNIVMRMVGGK++ ++ + DEG ++ +++V ++M +AG+TNPGDFIPLW
Sbjct: 181 LDLTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLW 240
Query: 241 NWIDPTGLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQII 300
NWIDP+G K+IMK+G DE+ Q L+DEIRNEE++GNTMI HLL LQ T P+Y+SD II
Sbjct: 241 NWIDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLII 300
Query: 301 KGLIHVILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSY 360
KGLI IL+AGIDT +VTL+W LSHLLNNP V+ KA+ EI+ +GQER+VNE DLSSLSY
Sbjct: 301 KGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSY 360
Query: 361 LQGIILETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSF 420
LQGII ETLRL+P PLLVPHCASEDC+I GYD+PR+TI+ +NAWAI RD ++WED SF
Sbjct: 361 LQGIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSF 420
Query: 421 KPERHENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDM 480
KPERH NA+ +SYKLLPFGLGRRACPG+ MAQRV+GLTLASLIQCF+W++M + LVDM
Sbjct: 421 KPERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDM 480
Query: 481 TEGQGITMPKAQPLVAKCKPRPIMRAHL 502
EG+G+TMPK + LVAKC+PR IM+ L
Sbjct: 481 REGEGLTMPKVESLVAKCRPRFIMKVVL 506
BLAST of Cucsa.073050 vs. TrEMBL
Match:
A0A0A0LNA4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425750 PE=3 SV=1)
HSP 1 Score: 676.4 bits (1744), Expect = 2.7e-191
Identity = 325/499 (65.13%), Postives = 409/499 (81.96%), Query Frame = 1
Query: 5 ILVYIFFFLISLLLGLKLLLH-HGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYG 64
+++ I L SLLL LL H +NLPPTP F LP+IGHLHL+KHP+H+ LH+LSK YG
Sbjct: 4 LILSISLSLFSLLLAFNFLLRPHRQNLPPTPFFCLPVIGHLHLIKHPLHRILHNLSKSYG 63
Query: 65 HVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELW 124
HVFSLRFGS LVV+ISSPS V+ECFTKNDIILANRP L+TGKHL+YN T +AVAPYGE W
Sbjct: 64 HVFSLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLDTGKHLAYNHTTMAVAPYGEHW 123
Query: 125 RRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFT 184
R LRRI E+FSTS++N FS +R++EVK L+ +LC +S+E F AVE+E +LLD
Sbjct: 124 RNLRRIGALELFSTSRINLFSRMREEEVKALMVRLCGSSSLEG---FRAVEMESMLLDLM 183
Query: 185 SNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEK 244
N++M M+GGK+ +D EG++++FR++V ++M GA+NPGDFI +WNWIDP+GL+K
Sbjct: 184 YNVIMGMMGGKKGCEED---EGKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGLKK 243
Query: 245 KIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVILLA 304
KI+K+G+ D + Q L+D +RNE +GNTMIH LL LQ +PE SDQIIKGLI +IL+A
Sbjct: 244 KILKLGQTMDVLLQELVDGMRNESGEGNTMIHRLLQLQKIEPENHSDQIIKGLIQIILIA 303
Query: 305 GIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETLR 364
GIDT +VTLEW LSHLLNNP V++KA++EI+++VGQ+RLVNE DL SL+YLQGII ETLR
Sbjct: 304 GIDTAAVTLEWALSHLLNNPDVLEKAKIEIDNVVGQKRLVNEADLPSLTYLQGIIFETLR 363
Query: 365 LTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHENAIE 424
L+PAAPLLVPHC+SEDC+I GYD+PRDTI+ +NAWAI RD +LWED TSFKPERH N I
Sbjct: 364 LSPAAPLLVPHCSSEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDATSFKPERHTNPIG 423
Query: 425 LSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPK 484
+ +SYK LPFGLGRRACPG+G+AQR+ LTLA++IQCF+W+R SSLVDM+EG+G+TMPK
Sbjct: 424 V-ESYKFLPFGLGRRACPGIGIAQRMTNLTLATMIQCFEWKREGSSLVDMSEGEGLTMPK 483
Query: 485 AQPLVAKCKPRPIMRAHLS 503
AQPL+AKCKPRPIM+A S
Sbjct: 484 AQPLIAKCKPRPIMKAMFS 495
BLAST of Cucsa.073050 vs. TrEMBL
Match:
W9RLY5_9ROSA (Isoflavone 2'-hydroxylase OS=Morus notabilis GN=L484_009301 PE=3 SV=1)
HSP 1 Score: 588.2 bits (1515), Expect = 9.6e-165
Identity = 298/505 (59.01%), Postives = 371/505 (73.47%), Query Frame = 1
Query: 1 MDNNILVYIFFFLISLLLGLKLLLHHGK---NLPPTPLFSLPLIGHLHLLKHPIHQTLHS 60
M+ +++Y FLI L+ KL L K NLPP+P SLP+IGHLHLLK PIH+T H
Sbjct: 1 MEETLVLYTCLFLIITLVAFKLFLQTKKPHKNLPPSPP-SLPIIGHLHLLKIPIHRTFHR 60
Query: 61 LSKKYGHVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVA 120
LS+KYG VFSLRFGS +VV+SSPSAV ECFTKNDI+LANRP L GKHL YN+T +AV+
Sbjct: 61 LSQKYGAVFSLRFGSRHLVVVSSPSAVEECFTKNDIVLANRPRLLMGKHLGYNYTTVAVS 120
Query: 121 PYGELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEP 180
PYG+ WR LRRI EIFS+ +LN+FS IR+DE+KRLL +L RNS++ F+ V+L
Sbjct: 121 PYGDHWRNLRRIGALEIFSSGRLNAFSGIRRDEIKRLLRRL-TRNSLQH---FARVDL-- 180
Query: 181 ILLDFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWID 240
+ T NI++RM+ GKRY+GD+V DE +A FRD+VK+ Y GA NPGDF+P+ NW
Sbjct: 181 VFSGLTFNIILRMLTGKRYYGDEVSDEEEARLFRDIVKKASAYGGAANPGDFLPILNWFG 240
Query: 241 PTGLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLI 300
EK++ ++ ++ D QGLID+ R +E N+++ HLL LQ +QPEY++DQIIKG I
Sbjct: 241 SNSYEKRVRRLAKKTDAFLQGLIDQNRRMKERPNSILSHLLSLQESQPEYYTDQIIKGFI 300
Query: 301 HVILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGI 360
V+LLAG +T SVTLEW +S+LLN+P V+KK + E++ VGQ+ LV E DLS L YLQ
Sbjct: 301 LVLLLAGTETSSVTLEWAMSNLLNHPHVLKKVKAELDTQVGQQNLVEEPDLSKLQYLQNT 360
Query: 361 ILETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPER 420
I ETLRL PA PLLVPH +S+DC I GY++PRDTI+ VNAWAI RD LW+D SFKPER
Sbjct: 361 ISETLRLYPATPLLVPHYSSDDCTIAGYNVPRDTILLVNAWAIHRDPKLWDDAESFKPER 420
Query: 421 HENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQ 480
E E KLLPFGLGRRACPG G+AQRV+GLTLASLIQCFDW R++ VDM EG+
Sbjct: 421 FETHHEGDQINKLLPFGLGRRACPGAGLAQRVVGLTLASLIQCFDWGRVNDEKVDMAEGR 480
Query: 481 GITMPKAQPLVAKCKPRPIMRAHLS 503
G+TMPKA PL A CK RPIM LS
Sbjct: 481 GLTMPKAVPLEAMCKARPIMNTVLS 498
BLAST of Cucsa.073050 vs. TrEMBL
Match:
W9R984_9ROSA (Putative receptor-like protein kinase OS=Morus notabilis GN=L484_009298 PE=4 SV=1)
HSP 1 Score: 582.8 bits (1501), Expect = 4.0e-163
Identity = 296/504 (58.73%), Postives = 373/504 (74.01%), Query Frame = 1
Query: 2 DNNILVYIFFFLISLLLGLKLLL---HHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSL 61
D N+++Y LI LL+ LKL L KNLPP+P +LP+IGHLHLL PIH+T L
Sbjct: 3 DPNLILYTSLPLIILLITLKLFLLTKKPQKNLPPSPP-ALPVIGHLHLLNSPIHRTFRDL 62
Query: 62 SKKYGHVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAP 121
S+KYG +FSLRFGS VVV+SSPSAV EC TKND++LANRP L T KH+SYN+T + +P
Sbjct: 63 SQKYGTIFSLRFGSRRVVVVSSPSAVEECCTKNDVVLANRPPLITAKHISYNYTTIVSSP 122
Query: 122 YGELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPI 181
YG+ WR LRRI T +IFS+S+LN F +R+DEVKRLL ++ ++NS+ VEL+ +
Sbjct: 123 YGDHWRNLRRIGTLQIFSSSRLNVFRGLRRDEVKRLLRRI-SQNSLRGS---GKVELKSM 182
Query: 182 LLDFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDP 241
L + T N++MRMV GKRY+GDDV D+ +A+KF++V+K V G NP DF+P+ NWI
Sbjct: 183 LSELTLNVIMRMVAGKRYYGDDVSDQDEAKKFKEVIKEVSENGGGANPADFVPILNWIWG 242
Query: 242 TGLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIH 301
G EKK+ ++ D I Q LIDE R+ +E NTMI HLL LQ +QPEY++DQIIKG
Sbjct: 243 -GFEKKMKRLAMRTDTILQSLIDEHRSRKESKNTMIDHLLSLQKSQPEYYTDQIIKGFAL 302
Query: 302 VILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGII 361
++LLAG DT SVTLEW +S+LLN+P ++KKA+ E++ VGQ+ LV E DLS L+YLQ II
Sbjct: 303 IMLLAGTDTSSVTLEWAMSNLLNHPSILKKAKAELDAQVGQQHLVEESDLSKLNYLQNII 362
Query: 362 LETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERH 421
ETLRL PA PLLVPH +S DC + GYD+PR T++F+NAWAI RD LWED SFKPER
Sbjct: 363 SETLRLYPAVPLLVPHYSSGDCTVGGYDVPRGTVLFINAWAIHRDPKLWEDAESFKPERF 422
Query: 422 ENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQG 481
EN S+SYKL PFGLGRR+CPG+G+AQRV+GLTL SLIQCF+WER+ VDM EG+G
Sbjct: 423 ENG--ESESYKLFPFGLGRRSCPGIGLAQRVVGLTLGSLIQCFEWERIGEEEVDMAEGKG 482
Query: 482 ITMPKAQPLVAKCKPRPIMRAHLS 503
+TMPKA PL A CK RPIM LS
Sbjct: 483 LTMPKAVPLEALCKARPIMNVVLS 498
BLAST of Cucsa.073050 vs. TAIR10
Match:
AT4G37370.1 (AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8)
HSP 1 Score: 498.0 bits (1281), Expect = 6.6e-141
Identity = 253/497 (50.91%), Postives = 349/497 (70.22%), Query Frame = 1
Query: 1 MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 60
M+ L++ F++ L+ L L NLPP+P +SLP+IGHL LLK PIH+T SLS+
Sbjct: 1 METKTLIFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQ 60
Query: 61 KYGH--VFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAP 120
+ +FSLR G+ LV V SS S ECFTKND++LANRP KH++Y++T + A
Sbjct: 61 SLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAAS 120
Query: 121 YGELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPI 180
YG+ WR LRRI + EIFS +LNSF IR+DE++RL+ +L +RN +E F V+++ +
Sbjct: 121 YGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRL-SRNFSQE---FVKVDMKSM 180
Query: 181 LLDFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDP 240
L D T N ++RMV GKRY+GD V D+ +A++ R ++ V+ AGA N D++P+ +
Sbjct: 181 LSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV-- 240
Query: 241 TGLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIH 300
+ E ++ K+ DE QGL+DE R +E GNTMI HLL LQ +QP+YF+D+IIKG +
Sbjct: 241 SDYETRVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNML 300
Query: 301 VILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGII 360
++LAG DT +VTLEW LS++LN+P V+ KAR EI+ +G +RL++E D+S+L YLQ I+
Sbjct: 301 ALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIV 360
Query: 361 LETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERH 420
ETLRL PAAP+L+PH ASEDC++ GYD+PR TI+ N WAI RD LW+D SFKPER
Sbjct: 361 SETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF 420
Query: 421 ENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQG 480
E ++ KL+PFGLGRRACPG G+A R++ LTL SLIQC +WE++ VDM+EG+G
Sbjct: 421 EKE---GEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKG 480
Query: 481 ITMPKAQPLVAKCKPRP 496
+TMPKA+PL A C+ RP
Sbjct: 481 VTMPKAKPLEAMCRARP 487
BLAST of Cucsa.073050 vs. TAIR10
Match:
AT5G36220.1 (AT5G36220.1 cytochrome p450 81d1)
HSP 1 Score: 497.7 bits (1280), Expect = 8.6e-141
Identity = 261/504 (51.79%), Postives = 345/504 (68.45%), Query Frame = 1
Query: 5 ILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYGH 64
+++Y F LI L++ K L +NLPP+P LP+IGHL LLK PIH+TL S S+ H
Sbjct: 8 VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67
Query: 65 -----VFSLRFGSYLVVVISSPS-AVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAP 124
V SLR GS LV V+SS A ECF KND++LANRP + GKH+ YN T + AP
Sbjct: 68 NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127
Query: 125 YGELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPI 184
YG+ WR LRR+ T EIFST +LN F +R DEV+RL+ +L ++ + VEL+P+
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK----TVVELKPM 187
Query: 185 LLDFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDP 244
L+D T N +MRM+ GKRY+G++ DE +A++ R +V V + N D++P+
Sbjct: 188 LMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF-- 247
Query: 245 TGLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIH 304
+ E ++ K+GEE D+ QGLID+ R ++E G TMI HLL LQ + EY++DQIIKG+I
Sbjct: 248 SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIIL 307
Query: 305 VILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGII 364
++++AG +T +VTLEW LS+LLN+P VI KAR EI++ VG +RL+ E DLS L YL+ I+
Sbjct: 308 IMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIV 367
Query: 365 LETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERH 424
LETLRL PA PLLVPH ASEDC+I YD+PR T + VNAWAI RD + W+D SFKPER
Sbjct: 368 LETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF 427
Query: 425 ENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQG 484
E ++ KLL FGLGRRACPG G+AQR++GL L SLIQCF+WER+ + VDM EG G
Sbjct: 428 EKE---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVG 487
Query: 485 ITMPKAQPLVAKCKPRPIMRAHLS 503
T+PKA PL A CK RP + +S
Sbjct: 488 NTVPKAIPLKAICKARPFLHKIIS 502
BLAST of Cucsa.073050 vs. TAIR10
Match:
AT4G37330.1 (AT4G37330.1 cytochrome P450, family 81, subfamily D, polypeptide 4)
HSP 1 Score: 493.8 bits (1270), Expect = 1.2e-139
Identity = 255/497 (51.31%), Postives = 338/497 (68.01%), Query Frame = 1
Query: 3 NNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKY 62
N IL+ F L +L + L NLPP+P SLP+IGHLHLLK P+H+T SLSK
Sbjct: 4 NQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSI 63
Query: 63 GH--VFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 122
G+ VF LR G+ LV VISS S ECFTKND++LANRP KHL YN T L A YG
Sbjct: 64 GNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYG 123
Query: 123 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL 182
+ WR LRRI+ EIFST +LNSF IR+DE++RL+ L S + L F VE++ +L
Sbjct: 124 DHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHL----SRDSLHGFVEVEMKTLLT 183
Query: 183 DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG 242
+ SN +RM+ GKRYFG+D D A+ +++V + AGA NP D++ + W+ +
Sbjct: 184 NLASNTTIRMLAGKRYFGEDNDD---AKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SS 243
Query: 243 LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI 302
EK+I +G D Q L+DE R E+E G TMI HLL LQ+ QP+Y++D IIKG+I +
Sbjct: 244 YEKRIKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTL 303
Query: 303 LLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE 362
++AG DT SVTLEW +S+LLN+P+++KKAR+EI+ VG +RLV+E D+ +LSYLQ I+LE
Sbjct: 304 IIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLE 363
Query: 363 TLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHEN 422
TLR+ PA PLL+PH +SEDC++ GYDIP T++ NAWA+ RD +WED FKPER E
Sbjct: 364 TLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEK 423
Query: 423 AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT 482
++ KL+ FG+GRRACPG G+A R++ L SL+QCF+WER+ VDMTE +G T
Sbjct: 424 E---GEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGAT 483
Query: 483 MPKAQPLVAKCKPRPIM 498
+PKA PL A CK R I+
Sbjct: 484 LPKAIPLRAMCKARSIV 488
BLAST of Cucsa.073050 vs. TAIR10
Match:
AT4G37320.1 (AT4G37320.1 cytochrome P450, family 81, subfamily D, polypeptide 5)
HSP 1 Score: 488.8 bits (1257), Expect = 4.0e-138
Identity = 246/498 (49.40%), Postives = 341/498 (68.47%), Query Frame = 1
Query: 5 ILVYIFFFLISLLLGLKLLL---HHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKK 64
IL+ F FL + +KLLL + NLPP+P + LP+IGHLHLLK P+H+T HS+SK
Sbjct: 6 ILLLSFLFLT---ISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKS 65
Query: 65 YGH--VFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPY 124
G+ +F LR G+ LV VISS S ECFTKND++LANRP + KH+ YNFT + A Y
Sbjct: 66 LGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASY 125
Query: 125 GELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPIL 184
G+ WR LRRI+ EIFS+ ++++FS IR+DE++RL+ L S + L F VEL+ +L
Sbjct: 126 GDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHL----SRDSLHGFVEVELKSLL 185
Query: 185 LDFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPT 244
+ N ++ MV GKRY+G D +A+ R+++ +M AG+ N D++P NW+ T
Sbjct: 186 TNLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--T 245
Query: 245 GLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHV 304
E + +G D + Q L+DE R E+E G T+I HLL Q T+PEY++D IIKG+I
Sbjct: 246 NFENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILA 305
Query: 305 ILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIIL 364
++LAG DT SVTLEW +S+LLN+P++++KAR EI+ +G +RLV E D+ +L YLQ I+
Sbjct: 306 LVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVS 365
Query: 365 ETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHE 424
ETLRL PA PLL+PH +S++C++ GYD+PR T++ N WA+ RD LWE+ FKPER E
Sbjct: 366 ETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFE 425
Query: 425 NAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGI 484
++ KL+PFG+GRRACPG + +R++ L L LIQ F+WER+ + LVDMTEG+GI
Sbjct: 426 KE---GEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGI 485
Query: 485 TMPKAQPLVAKCKPRPIM 498
TMPKA PL A CK R I+
Sbjct: 486 TMPKATPLRAMCKARAIV 491
BLAST of Cucsa.073050 vs. TAIR10
Match:
AT4G37340.1 (AT4G37340.1 cytochrome P450, family 81, subfamily D, polypeptide 3)
HSP 1 Score: 486.9 bits (1252), Expect = 1.5e-137
Identity = 254/501 (50.70%), Postives = 340/501 (67.86%), Query Frame = 1
Query: 1 MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 60
M+ L++ F F+ L + + NLPP+P ++LP+IGHL LLK P+H+ S+S+
Sbjct: 1 MEGQTLIFTFLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSE 60
Query: 61 KYGH--VFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAP 120
G + SLR G+ LV V+SS S ECFTKND++LANR KH+SY T + A
Sbjct: 61 SLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTAS 120
Query: 121 YGELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPI 180
YG+ WR LRRI EIFS +LNSFS IR+DE+ RL+ L +S+E F+ VE++ +
Sbjct: 121 YGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLE----FTKVEMKSM 180
Query: 181 LLDFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDP 240
+ T N ++RM+ GK Y+GD D+ +A++ R+++ M GA N D++P+ WI
Sbjct: 181 FSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI-- 240
Query: 241 TGLEKKIMKVGEEADEIFQGLIDEIRN-EEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLI 300
TG EK+I K+ DE QGL+DE R +E+ NTM+ HLL LQ TQPEY++D IIKG++
Sbjct: 241 TGSEKRIKKIASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIM 300
Query: 301 HVILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGI 360
++LAG DT +VTLEW LS LLN+P+++ KAR EI++ VG RLV E DLS L YLQ I
Sbjct: 301 LSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNI 360
Query: 361 ILETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPER 420
+ E+LRL PA+PLLVPH ASEDC++ GY +PR T++ NAWAI RD +W+D TSFKPER
Sbjct: 361 VSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPER 420
Query: 421 HENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTE-G 480
E ++ KLL FGLGRRACPG G+AQR+ LT+ SLIQCF+WER+ VDMTE G
Sbjct: 421 FEKE---GEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGG 480
Query: 481 QGITMPKAQPLVAKCKPRPIM 498
G+ MPKA PLVA CK RP++
Sbjct: 481 GGVIMPKAIPLVAMCKARPVV 492
BLAST of Cucsa.073050 vs. NCBI nr
Match:
gi|778673620|ref|XP_011650030.1| (PREDICTED: cytochrome P450 81D1-like [Cucumis sativus])
HSP 1 Score: 1004.2 bits (2595), Expect = 8.0e-290
Identity = 498/502 (99.20%), Postives = 499/502 (99.40%), Query Frame = 1
Query: 1 MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 60
MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK
Sbjct: 1 MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 60
Query: 61 KYGHVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 120
KYGHVFSLRFGS+LVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG
Sbjct: 61 KYGHVFSLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 120
Query: 121 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL 180
ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL
Sbjct: 121 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL 180
Query: 181 DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG 240
DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG
Sbjct: 181 DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG 240
Query: 241 LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI 300
LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI
Sbjct: 241 LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI 300
Query: 301 LLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE 360
LAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE
Sbjct: 301 FLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE 360
Query: 361 TLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHEN 420
TLRLTPAAPLLVPHCASEDCQIEGYDIPR TIIFVNAWAI RDSSLWEDVTSFKPERHEN
Sbjct: 361 TLRLTPAAPLLVPHCASEDCQIEGYDIPRGTIIFVNAWAIHRDSSLWEDVTSFKPERHEN 420
Query: 421 AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT 480
AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT
Sbjct: 421 AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT 480
Query: 481 MPKAQPLVAKCKPRPIMRAHLS 503
MPKAQPLVAKCKPRPIMRAHLS
Sbjct: 481 MPKAQPLVAKCKPRPIMRAHLS 502
BLAST of Cucsa.073050 vs. NCBI nr
Match:
gi|778674449|ref|XP_004151924.2| (PREDICTED: cytochrome P450 81D1-like [Cucumis sativus])
HSP 1 Score: 689.5 bits (1778), Expect = 4.4e-195
Identity = 338/508 (66.54%), Postives = 411/508 (80.91%), Query Frame = 1
Query: 1 MDNNILVYIFFFLISLLLGLKLLLH-HGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLS 60
MD + L+Y LISLLLG L H NLPPTPLF LP IGHLHLLKHP+H+TL +LS
Sbjct: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
Query: 61 KKYGHVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPY 120
+KYGHVFSL+FGS LV+++SSPSAV+ECFTKNDIILANRP LN+GK+LSYN T +AV+PY
Sbjct: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
Query: 121 GELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPIL 180
GE WR LRRI T EIFST++LNSFS IR++EVKRLL KLC E+ F VELE +L
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDE--FRVVELESML 180
Query: 181 LDFTSNIVMRMVGGKRYFGDD------VLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLW 240
LD TSNIVMRMVGGK++ ++ + DEG ++ +++V ++M +AG+TNPGDFIPLW
Sbjct: 181 LDLTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLW 240
Query: 241 NWIDPTGLEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQII 300
NWIDP+G K+IMK+G DE+ Q L+DEIRNEE++GNTMI HLL LQ T P+Y+SD II
Sbjct: 241 NWIDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLII 300
Query: 301 KGLIHVILLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSY 360
KGLI IL+AGIDT +VTL+W LSHLLNNP V+ KA+ EI+ +GQER+VNE DLSSLSY
Sbjct: 301 KGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSY 360
Query: 361 LQGIILETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSF 420
LQGII ETLRL+P PLLVPHCASEDC+I GYD+PR+TI+ +NAWAI RD ++WED SF
Sbjct: 361 LQGIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSF 420
Query: 421 KPERHENAIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDM 480
KPERH NA+ +SYKLLPFGLGRRACPG+ MAQRV+GLTLASLIQCF+W++M + LVDM
Sbjct: 421 KPERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDM 480
Query: 481 TEGQGITMPKAQPLVAKCKPRPIMRAHL 502
EG+G+TMPK + LVAKC+PR IM+ L
Sbjct: 481 REGEGLTMPKVESLVAKCRPRFIMKVVL 506
BLAST of Cucsa.073050 vs. NCBI nr
Match:
gi|778673623|ref|XP_011650031.1| (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus])
HSP 1 Score: 676.4 bits (1744), Expect = 3.8e-191
Identity = 325/499 (65.13%), Postives = 409/499 (81.96%), Query Frame = 1
Query: 5 ILVYIFFFLISLLLGLKLLLH-HGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYG 64
+++ I L SLLL LL H +NLPPTP F LP+IGHLHL+KHP+H+ LH+LSK YG
Sbjct: 4 LILSISLSLFSLLLAFNFLLRPHRQNLPPTPFFCLPVIGHLHLIKHPLHRILHNLSKSYG 63
Query: 65 HVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELW 124
HVFSLRFGS LVV+ISSPS V+ECFTKNDIILANRP L+TGKHL+YN T +AVAPYGE W
Sbjct: 64 HVFSLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLDTGKHLAYNHTTMAVAPYGEHW 123
Query: 125 RRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFT 184
R LRRI E+FSTS++N FS +R++EVK L+ +LC +S+E F AVE+E +LLD
Sbjct: 124 RNLRRIGALELFSTSRINLFSRMREEEVKALMVRLCGSSSLEG---FRAVEMESMLLDLM 183
Query: 185 SNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEK 244
N++M M+GGK+ +D EG++++FR++V ++M GA+NPGDFI +WNWIDP+GL+K
Sbjct: 184 YNVIMGMMGGKKGCEED---EGKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGLKK 243
Query: 245 KIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVILLA 304
KI+K+G+ D + Q L+D +RNE +GNTMIH LL LQ +PE SDQIIKGLI +IL+A
Sbjct: 244 KILKLGQTMDVLLQELVDGMRNESGEGNTMIHRLLQLQKIEPENHSDQIIKGLIQIILIA 303
Query: 305 GIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETLR 364
GIDT +VTLEW LSHLLNNP V++KA++EI+++VGQ+RLVNE DL SL+YLQGII ETLR
Sbjct: 304 GIDTAAVTLEWALSHLLNNPDVLEKAKIEIDNVVGQKRLVNEADLPSLTYLQGIIFETLR 363
Query: 365 LTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHENAIE 424
L+PAAPLLVPHC+SEDC+I GYD+PRDTI+ +NAWAI RD +LWED TSFKPERH N I
Sbjct: 364 LSPAAPLLVPHCSSEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDATSFKPERHTNPIG 423
Query: 425 LSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPK 484
+ +SYK LPFGLGRRACPG+G+AQR+ LTLA++IQCF+W+R SSLVDM+EG+G+TMPK
Sbjct: 424 V-ESYKFLPFGLGRRACPGIGIAQRMTNLTLATMIQCFEWKREGSSLVDMSEGEGLTMPK 483
Query: 485 AQPLVAKCKPRPIMRAHLS 503
AQPL+AKCKPRPIM+A S
Sbjct: 484 AQPLIAKCKPRPIMKAMFS 495
BLAST of Cucsa.073050 vs. NCBI nr
Match:
gi|659111602|ref|XP_008455814.1| (PREDICTED: LOW QUALITY PROTEIN: isoflavone 2'-hydroxylase-like [Cucumis melo])
HSP 1 Score: 675.6 bits (1742), Expect = 6.5e-191
Identity = 326/500 (65.20%), Postives = 410/500 (82.00%), Query Frame = 1
Query: 5 ILVYIFFFLISLLLGLKLLLH-HGKNLPPTPLFS-LPLIGHLHLLKHPIHQTLHSLSKKY 64
+++ I L SLLL L LL H +NLPPTP F LP+IGHLHL+KHP+H+ LH+LSKKY
Sbjct: 4 LILSISLSLFSLLLALNFLLRPHRQNLPPTPFFPXLPIIGHLHLIKHPLHRILHNLSKKY 63
Query: 65 GHVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGEL 124
GHVFSLRFGS LVV+ISSPS V+ECFTKNDIILANRP L TGKHL+YN T +AVAPYGE
Sbjct: 64 GHVFSLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLGTGKHLAYNHTTMAVAPYGEH 123
Query: 125 WRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDF 184
WR LRRI E+FSTS++N FS +R++EVK L+ +LC +S+E F AVE+E +LLD
Sbjct: 124 WRNLRRIGALELFSTSRINLFSRMREEEVKALMVRLCGSSSLEG---FRAVEMESMLLDL 183
Query: 185 TSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLE 244
N++MRM+GGK+ D DEG++++FR++V ++M GA+NPGDFI +WNWIDP+G +
Sbjct: 184 MYNVIMRMMGGKK---DCEEDEGKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGWK 243
Query: 245 KKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVILL 304
KKIMK+G+ D + Q L+D IRNE +GNTM+H LLHLQ +PE SDQIIKGLI +IL+
Sbjct: 244 KKIMKLGQTMDVLLQDLVDGIRNENGEGNTMLHRLLHLQKIEPENHSDQIIKGLIQIILI 303
Query: 305 AGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETL 364
AG+DT +VTLEW L+HLLNNP V++KA+LEI+++VGQERLV+E DL SL+YLQGII ETL
Sbjct: 304 AGVDTTAVTLEWALAHLLNNPDVLQKAKLEIDNVVGQERLVSEVDLPSLTYLQGIIFETL 363
Query: 365 RLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHENAI 424
RL+PAAPLLVPHC+ EDC+I GYD+PRDTI+ +NAWAI RD +LWED +SFKPERH N I
Sbjct: 364 RLSPAAPLLVPHCSFEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDASSFKPERHTNPI 423
Query: 425 ELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMP 484
+ +SYK +PFGLGRRACPG+G+AQ++ LTLA++IQCF+WER SSLVDM+EG+G+TMP
Sbjct: 424 GV-ESYKFIPFGLGRRACPGIGIAQKMANLTLATMIQCFEWEREGSSLVDMSEGEGLTMP 483
Query: 485 KAQPLVAKCKPRPIMRAHLS 503
KAQPL+AKCKPRPIM+A S
Sbjct: 484 KAQPLIAKCKPRPIMKAVFS 496
BLAST of Cucsa.073050 vs. NCBI nr
Match:
gi|659111621|ref|XP_008455824.1| (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])
HSP 1 Score: 669.5 bits (1726), Expect = 4.7e-189
Identity = 325/499 (65.13%), Postives = 397/499 (79.56%), Query Frame = 1
Query: 5 ILVYIFFFLISLLLGLKLLLH-HGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYG 64
+L+ I FFL+S++ K LL NLPPTPLF LPLIGHLHLLKHP+HQTL +LS+KYG
Sbjct: 4 LLLSISFFLLSVVFAFKFLLRPRWLNLPPTPLFWLPLIGHLHLLKHPVHQTLQNLSQKYG 63
Query: 65 HVFSLRFGSYLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELW 124
HVFSLRFGS LVVV+SSPSAV+ECFTKNDIILANRP L +GK+L YN + + ++PYGE W
Sbjct: 64 HVFSLRFGSRLVVVVSSPSAVQECFTKNDIILANRPLLESGKYLGYNHSSIGLSPYGEQW 123
Query: 125 RRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFT 184
R LRRI + E+FST +LN F IR+DEVKRLL KLC + E F VE+ P+L D T
Sbjct: 124 RNLRRIGSLEVFSTKRLNMFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLT 183
Query: 185 SNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEK 244
NIVMRMV GK ++ +D + KFR++V ++M +AGA+NPGDFIPLWNWIDPTG K
Sbjct: 184 LNIVMRMVSGKMFYEED------SNKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMK 243
Query: 245 KIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVILLA 304
K+ K+G+ +DE Q LID IRN+ + GNTMIHHLL LQN +PE+++D K +I ILLA
Sbjct: 244 KVKKLGKTSDEFLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQDILLA 303
Query: 305 GIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETLR 364
GIDT +VTL+W LSHLLNNP V++KA+ EI+ +GQER+VNE DLSSLSYLQGII ETLR
Sbjct: 304 GIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGIISETLR 363
Query: 365 LTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIQRDSSLWEDVTSFKPERHENAIE 424
L+PA PLLVPHC+SEDC+I GYD+PR+TI+ +NAWAI RD +LWED +SFKPERH NA
Sbjct: 364 LSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPERHVNAAG 423
Query: 425 LSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPK 484
+SYKLLPFG+GRRACPG+ MAQRV+GLTLASL+QCF+W+RM LVDM EG+G+TMPK
Sbjct: 424 FENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGEGLTMPK 483
Query: 485 AQPLVAKCKPRPIMRAHLS 503
+PLVAKC+P IM A LS
Sbjct: 484 VEPLVAKCRPCFIMEAVLS 496
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| C81E9_MEDTR | 8.1e-141 | 52.35 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1 | [more] |
| C81D1_ARATH | 1.5e-139 | 51.79 | Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1 | [more] |
| C81E8_MEDTR | 2.6e-139 | 52.13 | Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1 | [more] |
| C81E1_GLYEC | 8.4e-138 | 49.69 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2 | [more] |
| C81E7_MEDTR | 3.0e-135 | 48.70 | Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LTD0_CUCSA | 5.6e-290 | 99.20 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425740 PE=3 SV=1 | [more] |
| A0A0A0LQI5_CUCSA | 3.1e-195 | 66.54 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423640 PE=3 SV=1 | [more] |
| A0A0A0LNA4_CUCSA | 2.7e-191 | 65.13 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425750 PE=3 SV=1 | [more] |
| W9RLY5_9ROSA | 9.6e-165 | 59.01 | Isoflavone 2'-hydroxylase OS=Morus notabilis GN=L484_009301 PE=3 SV=1 | [more] |
| W9R984_9ROSA | 4.0e-163 | 58.73 | Putative receptor-like protein kinase OS=Morus notabilis GN=L484_009298 PE=4 SV=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G37370.1 | 6.6e-141 | 50.91 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
| AT5G36220.1 | 8.6e-141 | 51.79 | cytochrome p450 81d1 | [more] |
| AT4G37330.1 | 1.2e-139 | 51.31 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
| AT4G37320.1 | 4.0e-138 | 49.40 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |
| AT4G37340.1 | 1.5e-137 | 50.70 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
