Cucsa.061550 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.061550 vs. Swiss-Prot
Match: PTR9_ARATH (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 1.4e-181
Identity = 313/553 (56.60%), Postives = 412/553 (74.50%), Query Frame = 1

Query: 2   ELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTG 61
           E  TPLL+ TV    D++ +P ++  SGGWRSA F+IGVEVAERFAYYGI SNLITYLTG
Sbjct: 9   EAGTPLLAVTV----DYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTG 68

Query: 62  PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAM 121
           PLGQS A AA  VN WSG + LL LLGAF+ADSF GR+RTIL +SA+Y++GL +L+ SAM
Sbjct: 69  PLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAM 128

Query: 122 LPTT-------SSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSK 181
           +P+        SS + +FQ++ FF +LYL+ + QGGHKPCVQAFGADQFD   P+E K+K
Sbjct: 129 IPSDCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAK 188

Query: 182 SSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSI 241
           SSFFNWW+FG+C GT   + ++ Y ++NLSW+LGFGIPCI M++A  + L GT TYR+SI
Sbjct: 189 SSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSI 248

Query: 242 KIYAQTPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDS 301
           +   Q+PF+RIG V+V+A++N   S+  +   E     +S  ++ QF FLNKA +    S
Sbjct: 249 RREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNGS 308

Query: 302 DKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIP 361
                 CS  E+EEAK+VLR+ P+W+T LV+A+VFAQ  TFFTKQGAT++RSI  G+ I 
Sbjct: 309 ------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKIS 368

Query: 362 AAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMV 421
            A L SF+ LSIVIFI IYD + +PIAR+FT    GIT LQRIGTG+ +S  +M+VAA+V
Sbjct: 369 PATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALV 428

Query: 422 ERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKS 481
           E KRL+ A ++GLVD PD  +PMS WWLVPQY LFG+ +VF +VGLQEFFYDQVP +L+S
Sbjct: 429 EMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRS 488

Query: 482 MGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVA 541
           +GLA Y S+ G+G+ LSS ++S I++ T  S Q SWF+NNLN+AHLDYFY+LL+ LS + 
Sbjct: 489 VGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFIG 548

Query: 542 FVAFLFVSKSHTA 548
             ++L+V+KS+ +
Sbjct: 549 LASYLYVAKSYVS 551

BLAST of Cucsa.061550 vs. Swiss-Prot
Match: PTR23_ARATH (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 605.9 bits (1561), Expect = 4.5e-172
Identity = 307/547 (56.12%), Postives = 394/547 (72.03%), Query Frame = 1

Query: 5   TPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           T L  + V  AVD +G    R  +G WR+A F+IGVEVAERFA YGIGSNLI+YLTGPLG
Sbjct: 7   TSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLG 66

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAML-- 124
           QS A AA  VN WSGIS +L LLGAF+AD+F GRY TI+ +S IYVLGL+ L+ SA L  
Sbjct: 67  QSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIP 126

Query: 125 --PTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 184
                +S  S F   LFF SLYL+ IGQ GHKPCVQAFGADQFD  +PQE   +SSFFNW
Sbjct: 127 NNTEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNW 186

Query: 185 WFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQ- 244
           W+  +CAG  +AIL+V Y +EN+SW+LGFGIPC+ M+I+  LF+ G  +YR+S     + 
Sbjct: 187 WYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEE 246

Query: 245 -TPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDSDKHG 304
             PF RIGRVF  A +N R +S+ +   E    + SQ++  +  FLNKA +VP DSD+  
Sbjct: 247 TNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPNDSDEGE 306

Query: 305 VMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIPAAAL 364
           V C + +VE+A A++R+ PVW+T L +AI FAQ  TFFTKQG T++R+I  G  IP A+L
Sbjct: 307 VACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASL 366

Query: 365 DSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMVERKR 424
              + +SIV+F+ IYD + VPI R+ T    GITTL+RIGTG+V++  +M+VAA+VE KR
Sbjct: 367 QVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESKR 426

Query: 425 LRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLA 484
           L  A E+GL+D+P   +PMS WWL PQY L GLA+V TLVG+QEFFY QVPT+L+S+GLA
Sbjct: 427 LETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLA 486

Query: 485 FYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVAFVAF 544
            Y S +G+GS+LSSLL+  ID  TGG   NSWF++NLN+AHLDYFY+LL+ +S V F  F
Sbjct: 487 IYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFTF 546

Query: 545 LFVSKSH 546
           LF+SKS+
Sbjct: 547 LFISKSY 553

BLAST of Cucsa.061550 vs. Swiss-Prot
Match: PTR22_ARATH (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 605.1 bits (1559), Expect = 7.6e-172
Identity = 308/545 (56.51%), Postives = 393/545 (72.11%), Query Frame = 1

Query: 7   LLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQS 66
           L  + V  AVD +G    R  +G WR+A F+IGVEVAERFAYYGIGSNLI+YLTGPLG+S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 67  VATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAML-PTT 126
            A AA  VN WSGI+ LL +LGAF+AD+F GRYRTI+ SS IYVLGL+ L+ SA L P T
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNT 128

Query: 127 S---SQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNWWF 186
           +   S  S F  +LFF SLYL+ IGQ GHKPCVQAFGADQFD    QE   +SSFFNWW+
Sbjct: 129 TEVTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWY 188

Query: 187 FGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQ--T 246
             + AG   AIL+V Y +E  SW+ GFGIPC+ M+I+  LF+ G   YRYS + + +   
Sbjct: 189 LSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEIN 248

Query: 247 PFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDSDKHGVM 306
           PF RIGRVF  A++N R SS+ +   E +     ++ +    F NKA +VP DS +    
Sbjct: 249 PFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQS----FFNKALLVPNDSSQGENA 308

Query: 307 CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIPAAALDS 366
             +S+VE+A A++R+ PVW T L +AI +AQ  TFFTKQG T+DR+I  G  IP A+L  
Sbjct: 309 SKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQV 368

Query: 367 FVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMVERKRLR 426
           F+ +SIV+F+ IYD +FVPIAR  T    GITTL+RIGTG+V+S  +M++AA+VE KRL 
Sbjct: 369 FIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLE 428

Query: 427 VADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLAFY 486
            A EHGL+D+P+  +PMS WWL+PQY L GLA+V+TLVG+QEFFY QVPT+L+S+GLA Y
Sbjct: 429 TAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALY 488

Query: 487 TSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVAFVAFLF 546
            S LG+GS+LSSLL+S ID  TGG   NSWF++NLN+AHLDYFY+LL+ +S V F  FLF
Sbjct: 489 LSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTFLF 548

BLAST of Cucsa.061550 vs. Swiss-Prot
Match: PTR25_ARATH (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 2.2e-158
Identity = 288/552 (52.17%), Postives = 383/552 (69.38%), Query Frame = 1

Query: 9   SQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQSVA 68
           ++ VE +VDF+G P +R  SG W+S+ F +  EVAE+FAY+GI SNLITY T  LG+S A
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 69  TAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLP----- 128
            AA  VN+W G +  L L+   +ADSF GR+RTIL +S+ Y++GL LL+FSA +P     
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSLCND 135

Query: 129 --TTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNWW 188
             T  S  SQ ++++FF +LYLI +G+GG K C++AFGADQFD   P E+K+KSS+FNW 
Sbjct: 136 QETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSYFNWL 195

Query: 189 FFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQ-- 248
           +F I  G     L+  Y +ENLSW+LG+ IPC+ M++A FLFL G  TYR+S     +  
Sbjct: 196 YFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGEGRQG 255

Query: 249 ----TPFLRIGRVFVSAIRNCRA--SSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKD 308
                PF+RIGRVFV+A RN R   S T +         L  ++  +FRFL++A I    
Sbjct: 256 KKHDNPFVRIGRVFVAAARNRRQTPSDTCLL--------LPNESTKKFRFLDRAVI---- 315

Query: 309 SDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFII 368
                  C + EVEEAKAVL + P+W+  LVF IVFAQ  TFFTKQG+T+DRSI S   +
Sbjct: 316 ------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTLQV 375

Query: 369 PAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAM 428
           PAA L  F+ L+I++FI IYD LFVPIAR+ T   +GITTLQRI TG+ +S  SM++AA+
Sbjct: 376 PAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIAAL 435

Query: 429 VERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLK 488
           VE KRL+ A +HGLVD P   +PMS  WL+PQY LFG+++VFT+VGLQEFFY +VP  L+
Sbjct: 436 VEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQLR 495

Query: 489 SMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVV 546
           SMGLA Y S++G+G+ LSS +VS I+E T  S Q SWFSNNLN+AHLDYFY+LL+ LS +
Sbjct: 496 SMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLSSL 549

BLAST of Cucsa.061550 vs. Swiss-Prot
Match: PTR11_ARATH (Protein NRT1/ PTR FAMILY 5.15 OS=Arabidopsis thaliana GN=NPF5.15 PE=2 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 8.2e-158
Identity = 286/556 (51.44%), Postives = 387/556 (69.60%), Query Frame = 1

Query: 7   LLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQS 66
           LL   V  +VD +G P  +  +GGWRSA F+IGVEVAERFAY+GI  NLITYLTGPLGQS
Sbjct: 10  LLEDYVSDSVDHRGFPAGKSSTGGWRSAWFIIGVEVAERFAYFGIACNLITYLTGPLGQS 69

Query: 67  VATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAML---- 126
            A AA  VN WSG + +L +LGAF+AD++ GRYRTI+ +S IY+LGL LL+ SA L    
Sbjct: 70  TAKAAVNVNTWSGTASILPILGAFVADAYLGRYRTIVVASLIYILGLGLLTLSASLIIMG 129

Query: 127 -----PTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSF 186
                   S++ S +   LFF SLYL+ IGQGGHKPCVQAFGADQFDA  P+E  ++ SF
Sbjct: 130 LSKQRNDASAKPSIWVNTLFFCSLYLVAIGQGGHKPCVQAFGADQFDAEDPKEVIARGSF 189

Query: 187 FNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSI--- 246
           FNWWF  + AG  ++I++V Y +EN++W+ GFGIPC+ M++A  +FL G   YRY     
Sbjct: 190 FNWWFLSLSAGISISIIVVAYVQENVNWAFGFGIPCLFMVMALAIFLLGRKIYRYPKGHH 249

Query: 247 -KIYAQTPFLRIGRVFVSAIRNCRAS---STVIFDEEGDGPDLSQQNAGQFRFLNKACIV 306
            ++ +   F RIGRVFV A +N +     S++  D+       S++   +  FL KA I 
Sbjct: 250 EEVNSSNTFARIGRVFVIAFKNRKLRLEHSSLELDQGLLEDGQSEKRKDRLNFLAKAMI- 309

Query: 307 PKDSDKHGVM-CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRS 366
                + GV  CS  +V++AKA++R+ P+WIT +V  I +AQ  TFFTKQG T+DR I  
Sbjct: 310 ----SREGVEPCSGRDVDDAKALVRLIPIWITYVVSTIPYAQYITFFTKQGVTVDRRILP 369

Query: 367 GFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSML 426
           G  IPAA+L SFV +SI+I + +Y+ +F+PIAR  T    GIT LQRIG G+V+S F+M+
Sbjct: 370 GVEIPAASLLSFVGVSILISVPLYERVFLPIARKITKKPFGITMLQRIGAGMVLSVFNMM 429

Query: 427 VAAMVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVP 486
           +AA+VE KRL++A EHGLVD+PD+ +PMS WW VPQY L G+ ++F++VG QEFFYDQVP
Sbjct: 430 LAALVESKRLKIAREHGLVDKPDVTVPMSIWWFVPQYLLLGMIDLFSMVGTQEFFYDQVP 489

Query: 487 TDLKSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSG 546
           T+L+S+GL+   S +G+ S LS  L+S ID  TG   ++ WF++NLN+AH+DYFY+LL+ 
Sbjct: 490 TELRSIGLSLSLSAMGLSSFLSGFLISLIDWATG---KDGWFNSNLNRAHVDYFYWLLAA 549

BLAST of Cucsa.061550 vs. TrEMBL
Match: A0A067K512_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20272 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 1.9e-198
Identity = 336/557 (60.32%), Postives = 430/557 (77.20%), Query Frame = 1

Query: 2   ELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTG 61
           ++ TPLL  TVE AVD+KGRPV R  SGGWRSASF+IGVE+AERFAYYGI SNLITYLTG
Sbjct: 9   DIQTPLLYDTVEPAVDYKGRPVFRSRSGGWRSASFIIGVEIAERFAYYGIASNLITYLTG 68

Query: 62  PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAM 121
            LGQS ATAAE VN WSG + LL L GAF+ADSF GRYRTI+ +S IY+LGL LL+ SAM
Sbjct: 69  SLGQSTATAAENVNAWSGTASLLPLFGAFVADSFLGRYRTIVVASLIYILGLGLLTLSAM 128

Query: 122 LPTTSSQN-------------SQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHP 181
           L ++ S +             S  Q++LFF SLYL+ +GQGGHKPCVQAFGADQFD   P
Sbjct: 129 LASSHSASDCPSTNIIKSCSPSSCQVVLFFFSLYLVALGQGGHKPCVQAFGADQFDGQDP 188

Query: 182 QEAKSKSSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTN 241
           +E KSKSSFFNWW+F +C GT V++L + Y ++NL+W+LGFGIPCIMM++A F+FL GT 
Sbjct: 189 EECKSKSSFFNWWYFCMCGGTLVSLLFLNYIQDNLNWALGFGIPCIMMVVALFIFLVGTK 248

Query: 242 TYRYSIKIYAQTPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKAC 301
           TYRYS+K+  ++ FLRIGRVFV+ ++N +   + I  EE     L   ++ QF+FLNKA 
Sbjct: 249 TYRYSVKVEERSAFLRIGRVFVATVKNWQTLPSAIDLEEEARGILPHHSSDQFKFLNKAL 308

Query: 302 IVPKDSDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIR 361
           + P  S + G +CS  EVEEAKAVLR+ P+W T L++A+VFAQ +TFFTKQG T+DRSI 
Sbjct: 309 VAPNGSKEGGKVCSLGEVEEAKAVLRLVPIWFTCLIYAVVFAQSATFFTKQGVTMDRSIS 368

Query: 362 SGFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSM 421
            GF +P A+L SF+ L+IV+F+ IYD +FVPIAR  T   SGIT LQRIGTG+ +SA SM
Sbjct: 369 KGFDVPPASLQSFISLAIVLFVPIYDRVFVPIARTLTRKPSGITMLQRIGTGMFLSALSM 428

Query: 422 LVAAMVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQV 481
            +AA+VE +RL ++ E+GLVD P++ +PMS WWL+PQY LFG+A+VFT+VGLQEFFYDQV
Sbjct: 429 AIAALVEMRRLEISREYGLVDMPNVTVPMSVWWLIPQYVLFGVADVFTMVGLQEFFYDQV 488

Query: 482 PTDLKSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLS 541
           P++L+S+GL+ Y  + G+GS +SS LVS I++ TGG   +SWFSNNLN+AHLDYFY+LL+
Sbjct: 489 PSELRSVGLSLYLGIFGVGSFISSFLVSAIEKATGGEGHDSWFSNNLNRAHLDYFYWLLA 548

Query: 542 GLSVVAFVAFLFVSKSH 546
           GLS+V  +AFL+ +K +
Sbjct: 549 GLSIVQLIAFLYFAKRY 565

BLAST of Cucsa.061550 vs. TrEMBL
Match: M5VYB7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003472mg PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.8e-197
Identity = 340/552 (61.59%), Postives = 427/552 (77.36%), Query Frame = 1

Query: 5   TPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           TPLL   VEGAVD+KG PV R  SGGWRSA F+IGVEVAERFAYYGI  NLIT+LTGPLG
Sbjct: 13  TPLLDDVVEGAVDYKGLPVHRSRSGGWRSAWFIIGVEVAERFAYYGISCNLITFLTGPLG 72

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLPT 124
           QS ATAAE VNIWSG + LL LLGAF+ADSF GRYRTI+F+S +Y+LGL LL+ SA+LP+
Sbjct: 73  QSTATAAENVNIWSGTASLLPLLGAFVADSFLGRYRTIVFASLLYILGLGLLTLSAVLPS 132

Query: 125 -----------TSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKS 184
                      ++S +SQFQ+L FF SLYL+ + QGGHKPCVQAFGADQFDA  P+E K+
Sbjct: 133 LTGSYCQKFNQSTSCSSQFQVLFFFFSLYLVAVAQGGHKPCVQAFGADQFDASDPEECKA 192

Query: 185 KSSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYS 244
           KSSFFNWW+F +C GT   ++L+TY ++NLSW LGFGIPCI M++A F+FL GT TYR S
Sbjct: 193 KSSFFNWWYFSLCFGTTFTLILLTYIQDNLSWGLGFGIPCIAMVLALFIFLLGTRTYRCS 252

Query: 245 IKIYAQTPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKD 304
           IK   ++PF+RIGRVFV+A+RN R + + I  EE     L  +++ QF+FLNKA + P D
Sbjct: 253 IKGDEESPFVRIGRVFVAALRNWRTTPSAITCEEESRGTLPHKSSEQFKFLNKALLAPDD 312

Query: 305 SDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFII 364
             +   +C+ +EVEEAK+VLR+FP+W+T L +A+VFAQ STFFTKQGAT+DR+I  G  I
Sbjct: 313 LKESRKVCTVAEVEEAKSVLRLFPIWVTCLAYAVVFAQYSTFFTKQGATMDRTIAPGLDI 372

Query: 365 PAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAM 424
           PAA+L +F+ ++IVIF+ IYD +FVPIAR FT   SGIT LQRIG G+ +S  SM+VAA+
Sbjct: 373 PAASLQTFISITIVIFVPIYDRIFVPIARVFTRKPSGITMLQRIGIGMFISIISMVVAAL 432

Query: 425 VERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLK 484
           VE KRL+ A ++GL+D P   +PMS WWLVPQY L GLA+VFT+VGLQEFFYDQVP +LK
Sbjct: 433 VEMKRLKTAKDYGLLDMPSATVPMSIWWLVPQYLLAGLADVFTMVGLQEFFYDQVPNELK 492

Query: 485 SMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVV 544
           S+GLA Y S+ G+GS LSS L+S ID+ T  + + SWFS NLN+AHLDYFY+LL G+SVV
Sbjct: 493 SIGLALYLSIFGVGSFLSSFLISAIDDATTWAGETSWFSTNLNRAHLDYFYWLLGGISVV 552

Query: 545 AFVAFLFVSKSH 546
               +L+ +KS+
Sbjct: 553 ELAIYLYFAKSY 564

BLAST of Cucsa.061550 vs. TrEMBL
Match: A0A0A0KSZ8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G652240 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 6.3e-197
Identity = 356/575 (61.91%), Postives = 435/575 (75.65%), Query Frame = 1

Query: 1   MELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           M+  TPLL+  V+GAVD+KG+P+LR  S  WRSASF+IG+EVAERF++YGIG+NLITYLT
Sbjct: 7   MDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFSFYGIGANLITYLT 66

Query: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120
           GPLG SV  AAE VN+W+  SMLLTL GAFLADSFFGRYRTI+F+S  Y+LGL LL  S 
Sbjct: 67  GPLGISVVAAAEIVNVWAATSMLLTLFGAFLADSFFGRYRTIVFASISYILGLGLLVLST 126

Query: 121 MLPTTSSQ------------NSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHP 180
           +LPT SS             + +  L+LFF+SL LIGI QGGH+PCVQAFGADQFD  +P
Sbjct: 127 LLPTPSSSICAILNKFTPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNP 186

Query: 181 QEAKSKSSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTN 240
           QEAK KSSFFNWW+FG C G   AI  V+Y +ENLSW++GFGIPC+ M+    LFL GT 
Sbjct: 187 QEAKFKSSFFNWWYFGACFGIVAAIPTVSYAQENLSWAVGFGIPCVSMLTGFILFLLGTE 246

Query: 241 TYRYS-IKIYAQTPFLRIGRVFVSAIRNCRAS-STVIFDEEGDGPDLSQQNAGQF----- 300
           TYR++ +K   ++PF RIGRVF+++IRN RAS ST+ F +E     LS     +      
Sbjct: 247 TYRFNTLKQSDKSPFWRIGRVFLASIRNLRASASTITFQDEQTAKYLSTSQQFKMHIMPT 306

Query: 301 ----------RFLNKACI-VPKDSDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFA 360
                     RFLNKACI VP DS+++ + CS SE+EEAKA+LRI  +W+TV+VF I F+
Sbjct: 307 EDASFHVWCDRFLNKACIIVPIDSNQNAMTCSVSELEEAKAILRILIIWVTVVVFTIAFS 366

Query: 361 QDSTFFTKQGATIDRSIRSGFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSG 420
           QD+TFFTKQ AT+DRSI SGFIIPAA+L++ +  +IVIFI +YDLLFVPIA+  TG  SG
Sbjct: 367 QDATFFTKQAATLDRSIMSGFIIPAASLEALISFTIVIFIVVYDLLFVPIAKKVTGNPSG 426

Query: 421 ITTLQRIGTGLVVSAFSMLVAAMVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFG 480
           ITTLQRIGTG+V+S  SM VA++VE+KRL+ A +HGLVD P++ IPM FWWLVPQY L G
Sbjct: 427 ITTLQRIGTGMVISTISMAVASLVEKKRLKTALDHGLVDTPEMTIPMRFWWLVPQYVLNG 486

Query: 481 LAEVFTLVGLQEFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSW 540
           LA+VFT+VGLQE  YDQVP DLKS+G A + S+LGMG+ILSSLL+S ID  T  +   SW
Sbjct: 487 LADVFTVVGLQELCYDQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSW 546

Query: 541 FSNNLNKAHLDYFYFLLSGLSVVAFVAFLFVSKSH 546
           FSNNLNKAHLDYFY LL+ LSV  FVAFLFV+KSH
Sbjct: 547 FSNNLNKAHLDYFYLLLAALSVFGFVAFLFVAKSH 581

BLAST of Cucsa.061550 vs. TrEMBL
Match: U5FXL3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s11120g PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 4.1e-196
Identity = 342/553 (61.84%), Postives = 427/553 (77.22%), Query Frame = 1

Query: 5   TPLLS-QTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPL 64
           TPLLS   V+G+VD+K RP  R  SGGWRSASF+IGVEVAERFAYYGI SNLITYLTGPL
Sbjct: 7   TPLLSYDIVDGSVDYKSRPAYRSNSGGWRSASFIIGVEVAERFAYYGISSNLITYLTGPL 66

Query: 65  GQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLP 124
           GQS ATAAE VN+W+G + LL LLGAF+ADSF GRYRTI+F+S IY+LGL LL+ SA+LP
Sbjct: 67  GQSTATAAENVNVWAGTASLLPLLGAFVADSFLGRYRTIIFASLIYILGLGLLTLSALLP 126

Query: 125 TTSSQNS-----------QFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAK 184
           + ++               FQL+LFF +LYL+ IGQ GHKPCVQAFGADQFD   PQE+K
Sbjct: 127 SHAAATGFRSTNLPSSPVPFQLILFFFALYLVAIGQSGHKPCVQAFGADQFDGQDPQESK 186

Query: 185 SKSSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRY 244
           +KSSFFNWW+  +CAGT V +L++ Y ++NL+W LGFGIPC +M+IA  +FL GT TYRY
Sbjct: 187 AKSSFFNWWYCFMCAGTVVTLLVLNYIQDNLNWGLGFGIPCAVMVIALIIFLLGTKTYRY 246

Query: 245 SIKIYAQTPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPK 304
           SIK   ++ FLRIG VFVS+IRN R + + I  EE     L  Q++ Q++FLNKA + P 
Sbjct: 247 SIKGDEKSAFLRIGWVFVSSIRNWRTTPSAIAFEEETRGTLPHQSSEQYKFLNKALLAPN 306

Query: 305 DSDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFI 364
              + G +CS  +VEEAKA+LR+ P+W T LVFAIVFAQ STFFTKQG T+DRSI     
Sbjct: 307 GLKEDGKVCSLGDVEEAKALLRLVPIWATCLVFAIVFAQTSTFFTKQGVTMDRSISPSLD 366

Query: 365 IPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAA 424
           +PAA+L SF+ LSIV+FI IYD + VPIARA T   SGIT LQRIG+G+ +S  +M+VAA
Sbjct: 367 LPAASLQSFISLSIVLFIPIYDRVLVPIARALTRKPSGITMLQRIGSGMFISVVAMIVAA 426

Query: 425 MVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDL 484
           +VE KRL+ A EHGLVD P++ IPMS WWL+PQY LFG+AE FT+VGLQEFFYDQVP+DL
Sbjct: 427 LVEMKRLKTAQEHGLVDLPNVTIPMSVWWLIPQYVLFGVAEAFTMVGLQEFFYDQVPSDL 486

Query: 485 KSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSV 544
           +S+GL+ Y S+ G+GS LSS L+S I++ TGG+ + SWF+NNLN+AHLDYFY+LL+GLS 
Sbjct: 487 RSVGLSLYLSIFGVGSFLSSFLISIIEKATGGNGRYSWFANNLNRAHLDYFYWLLAGLSA 546

Query: 545 VAFVAFLFVSKSH 546
           V  VA++  +KS+
Sbjct: 547 VQLVAYVCFAKSY 559

BLAST of Cucsa.061550 vs. TrEMBL
Match: A0A0B0P940_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_03296 PE=3 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 4.5e-195
Identity = 332/553 (60.04%), Postives = 427/553 (77.22%), Query Frame = 1

Query: 5   TPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           TPLL  T++ ++D+KGRPV R  SGGWRSASF+I VE+AERFAYYGI SNLITYLTGPLG
Sbjct: 11  TPLLDDTIDASLDYKGRPVRRSNSGGWRSASFIIAVEIAERFAYYGISSNLITYLTGPLG 70

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLPT 124
           +S A AA  VN WSG + LL LLGAF+ADSF GRYRTI+ +S +Y LGL LLS SA LP+
Sbjct: 71  ESTAAAAAQVNAWSGAASLLPLLGAFIADSFLGRYRTIILASLLYTLGLGLLSLSAALPS 130

Query: 125 TS------------SQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAK 184
            S            S ++QFQ++LFF SLYL+  GQGGHKPCVQAFGADQFD   P+E K
Sbjct: 131 VSNSGDQSTDNTILSSSAQFQVILFFFSLYLVAFGQGGHKPCVQAFGADQFDRQDPEECK 190

Query: 185 SKSSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRY 244
           ++SSFFNWW+FGICAGTF+ + ++TY ++NLSW+LGFGIP I+M++    F+ GT TYRY
Sbjct: 191 AQSSFFNWWYFGICAGTFITLWILTYIQDNLSWTLGFGIPAIVMVVGLLAFVLGTTTYRY 250

Query: 245 SIKIYAQTPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPK 304
           SIK   + PFLRIGRVF+ A+RN + +S+ I  EE     L  +++ QF+FLNKA + P 
Sbjct: 251 SIKGDEENPFLRIGRVFILAVRNWKTTSSAIAAEEEARGTLPTESSKQFKFLNKALLAPD 310

Query: 305 DSDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFI 364
            S + G +CS  EVEEAK V+R+ P+W   L +AIV+AQ  TFFTKQGAT+DRSI +GF 
Sbjct: 311 GSKEQGKVCSTGEVEEAKTVIRLAPIWAASLFYAIVYAQTITFFTKQGATMDRSITAGFK 370

Query: 365 IPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAA 424
           IPAA+L SF+ L+I+IFI IYD +FVP+ARA TG  +GIT LQRIGTG+++SA SML+AA
Sbjct: 371 IPAASLLSFISLTIMIFIPIYDRIFVPLARALTGKPAGITMLQRIGTGMLLSAISMLIAA 430

Query: 425 MVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDL 484
           +VE +RL+ A+E+GLVD+P++ +PMS WWL+PQY L GL++VF +VGLQEFFY QVP +L
Sbjct: 431 LVEMRRLKTAEEYGLVDKPNVTVPMSIWWLLPQYILSGLSDVFAMVGLQEFFYHQVPKEL 490

Query: 485 KSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSV 544
           +S+GLA Y S+ G+GS LSS L+S I+++TG   Q+SWF+NNLN+AHLDYFY+LL+ LS 
Sbjct: 491 RSVGLALYLSIFGVGSFLSSFLISAIEKMTGEGGQDSWFANNLNRAHLDYFYWLLAALSA 550

Query: 545 VAFVAFLFVSKSH 546
           +    FL  +KS+
Sbjct: 551 MGLALFLSSAKSY 563

BLAST of Cucsa.061550 vs. TAIR10
Match: AT1G22540.1 (AT1G22540.1 Major facilitator superfamily protein)

HSP 1 Score: 637.5 bits (1643), Expect = 7.8e-183
Identity = 313/553 (56.60%), Postives = 412/553 (74.50%), Query Frame = 1

Query: 2   ELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTG 61
           E  TPLL+ TV    D++ +P ++  SGGWRSA F+IGVEVAERFAYYGI SNLITYLTG
Sbjct: 9   EAGTPLLAVTV----DYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTG 68

Query: 62  PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAM 121
           PLGQS A AA  VN WSG + LL LLGAF+ADSF GR+RTIL +SA+Y++GL +L+ SAM
Sbjct: 69  PLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAM 128

Query: 122 LPTT-------SSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSK 181
           +P+        SS + +FQ++ FF +LYL+ + QGGHKPCVQAFGADQFD   P+E K+K
Sbjct: 129 IPSDCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAK 188

Query: 182 SSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSI 241
           SSFFNWW+FG+C GT   + ++ Y ++NLSW+LGFGIPCI M++A  + L GT TYR+SI
Sbjct: 189 SSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSI 248

Query: 242 KIYAQTPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDS 301
           +   Q+PF+RIG V+V+A++N   S+  +   E     +S  ++ QF FLNKA +    S
Sbjct: 249 RREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNGS 308

Query: 302 DKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIP 361
                 CS  E+EEAK+VLR+ P+W+T LV+A+VFAQ  TFFTKQGAT++RSI  G+ I 
Sbjct: 309 ------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKIS 368

Query: 362 AAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMV 421
            A L SF+ LSIVIFI IYD + +PIAR+FT    GIT LQRIGTG+ +S  +M+VAA+V
Sbjct: 369 PATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALV 428

Query: 422 ERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKS 481
           E KRL+ A ++GLVD PD  +PMS WWLVPQY LFG+ +VF +VGLQEFFYDQVP +L+S
Sbjct: 429 EMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRS 488

Query: 482 MGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVA 541
           +GLA Y S+ G+G+ LSS ++S I++ T  S Q SWF+NNLN+AHLDYFY+LL+ LS + 
Sbjct: 489 VGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFIG 548

Query: 542 FVAFLFVSKSHTA 548
             ++L+V+KS+ +
Sbjct: 549 LASYLYVAKSYVS 551

BLAST of Cucsa.061550 vs. TAIR10
Match: AT1G72125.1 (AT1G72125.1 Major facilitator superfamily protein)

HSP 1 Score: 605.9 bits (1561), Expect = 2.5e-173
Identity = 307/547 (56.12%), Postives = 394/547 (72.03%), Query Frame = 1

Query: 5   TPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           T L  + V  AVD +G    R  +G WR+A F+IGVEVAERFA YGIGSNLI+YLTGPLG
Sbjct: 7   TSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLG 66

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAML-- 124
           QS A AA  VN WSGIS +L LLGAF+AD+F GRY TI+ +S IYVLGL+ L+ SA L  
Sbjct: 67  QSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIP 126

Query: 125 --PTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 184
                +S  S F   LFF SLYL+ IGQ GHKPCVQAFGADQFD  +PQE   +SSFFNW
Sbjct: 127 NNTEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNW 186

Query: 185 WFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQ- 244
           W+  +CAG  +AIL+V Y +EN+SW+LGFGIPC+ M+I+  LF+ G  +YR+S     + 
Sbjct: 187 WYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEE 246

Query: 245 -TPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDSDKHG 304
             PF RIGRVF  A +N R +S+ +   E    + SQ++  +  FLNKA +VP DSD+  
Sbjct: 247 TNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPNDSDEGE 306

Query: 305 VMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIPAAAL 364
           V C + +VE+A A++R+ PVW+T L +AI FAQ  TFFTKQG T++R+I  G  IP A+L
Sbjct: 307 VACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASL 366

Query: 365 DSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMVERKR 424
              + +SIV+F+ IYD + VPI R+ T    GITTL+RIGTG+V++  +M+VAA+VE KR
Sbjct: 367 QVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESKR 426

Query: 425 LRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLA 484
           L  A E+GL+D+P   +PMS WWL PQY L GLA+V TLVG+QEFFY QVPT+L+S+GLA
Sbjct: 427 LETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLA 486

Query: 485 FYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVAFVAF 544
            Y S +G+GS+LSSLL+  ID  TGG   NSWF++NLN+AHLDYFY+LL+ +S V F  F
Sbjct: 487 IYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFTF 546

Query: 545 LFVSKSH 546
           LF+SKS+
Sbjct: 547 LFISKSY 553

BLAST of Cucsa.061550 vs. TAIR10
Match: AT1G72120.1 (AT1G72120.1 Major facilitator superfamily protein)

HSP 1 Score: 605.1 bits (1559), Expect = 4.3e-173
Identity = 308/545 (56.51%), Postives = 393/545 (72.11%), Query Frame = 1

Query: 7   LLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQS 66
           L  + V  AVD +G    R  +G WR+A F+IGVEVAERFAYYGIGSNLI+YLTGPLG+S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 67  VATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAML-PTT 126
            A AA  VN WSGI+ LL +LGAF+AD+F GRYRTI+ SS IYVLGL+ L+ SA L P T
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNT 128

Query: 127 S---SQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNWWF 186
           +   S  S F  +LFF SLYL+ IGQ GHKPCVQAFGADQFD    QE   +SSFFNWW+
Sbjct: 129 TEVTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWY 188

Query: 187 FGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQ--T 246
             + AG   AIL+V Y +E  SW+ GFGIPC+ M+I+  LF+ G   YRYS + + +   
Sbjct: 189 LSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEIN 248

Query: 247 PFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDSDKHGVM 306
           PF RIGRVF  A++N R SS+ +   E +     ++ +    F NKA +VP DS +    
Sbjct: 249 PFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQS----FFNKALLVPNDSSQGENA 308

Query: 307 CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIPAAALDS 366
             +S+VE+A A++R+ PVW T L +AI +AQ  TFFTKQG T+DR+I  G  IP A+L  
Sbjct: 309 SKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQV 368

Query: 367 FVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMVERKRLR 426
           F+ +SIV+F+ IYD +FVPIAR  T    GITTL+RIGTG+V+S  +M++AA+VE KRL 
Sbjct: 369 FIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLE 428

Query: 427 VADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLAFY 486
            A EHGL+D+P+  +PMS WWL+PQY L GLA+V+TLVG+QEFFY QVPT+L+S+GLA Y
Sbjct: 429 TAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALY 488

Query: 487 TSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVAFVAFLF 546
            S LG+GS+LSSLL+S ID  TGG   NSWF++NLN+AHLDYFY+LL+ +S V F  FLF
Sbjct: 489 LSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTFLF 548

BLAST of Cucsa.061550 vs. TAIR10
Match: AT1G72140.1 (AT1G72140.1 Major facilitator superfamily protein)

HSP 1 Score: 560.5 bits (1443), Expect = 1.2e-159
Identity = 288/552 (52.17%), Postives = 383/552 (69.38%), Query Frame = 1

Query: 9   SQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQSVA 68
           ++ VE +VDF+G P +R  SG W+S+ F +  EVAE+FAY+GI SNLITY T  LG+S A
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 69  TAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLP----- 128
            AA  VN+W G +  L L+   +ADSF GR+RTIL +S+ Y++GL LL+FSA +P     
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSLCND 135

Query: 129 --TTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNWW 188
             T  S  SQ ++++FF +LYLI +G+GG K C++AFGADQFD   P E+K+KSS+FNW 
Sbjct: 136 QETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSYFNWL 195

Query: 189 FFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQ-- 248
           +F I  G     L+  Y +ENLSW+LG+ IPC+ M++A FLFL G  TYR+S     +  
Sbjct: 196 YFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGEGRQG 255

Query: 249 ----TPFLRIGRVFVSAIRNCRA--SSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKD 308
                PF+RIGRVFV+A RN R   S T +         L  ++  +FRFL++A I    
Sbjct: 256 KKHDNPFVRIGRVFVAAARNRRQTPSDTCLL--------LPNESTKKFRFLDRAVI---- 315

Query: 309 SDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFII 368
                  C + EVEEAKAVL + P+W+  LVF IVFAQ  TFFTKQG+T+DRSI S   +
Sbjct: 316 ------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTLQV 375

Query: 369 PAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAM 428
           PAA L  F+ L+I++FI IYD LFVPIAR+ T   +GITTLQRI TG+ +S  SM++AA+
Sbjct: 376 PAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIAAL 435

Query: 429 VERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLK 488
           VE KRL+ A +HGLVD P   +PMS  WL+PQY LFG+++VFT+VGLQEFFY +VP  L+
Sbjct: 436 VEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQLR 495

Query: 489 SMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVV 546
           SMGLA Y S++G+G+ LSS +VS I+E T  S Q SWFSNNLN+AHLDYFY+LL+ LS +
Sbjct: 496 SMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLSSL 549

BLAST of Cucsa.061550 vs. TAIR10
Match: AT1G22570.1 (AT1G22570.1 Major facilitator superfamily protein)

HSP 1 Score: 558.5 bits (1438), Expect = 4.6e-159
Identity = 286/556 (51.44%), Postives = 387/556 (69.60%), Query Frame = 1

Query: 7   LLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQS 66
           LL   V  +VD +G P  +  +GGWRSA F+IGVEVAERFAY+GI  NLITYLTGPLGQS
Sbjct: 10  LLEDYVSDSVDHRGFPAGKSSTGGWRSAWFIIGVEVAERFAYFGIACNLITYLTGPLGQS 69

Query: 67  VATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAML---- 126
            A AA  VN WSG + +L +LGAF+AD++ GRYRTI+ +S IY+LGL LL+ SA L    
Sbjct: 70  TAKAAVNVNTWSGTASILPILGAFVADAYLGRYRTIVVASLIYILGLGLLTLSASLIIMG 129

Query: 127 -----PTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSF 186
                   S++ S +   LFF SLYL+ IGQGGHKPCVQAFGADQFDA  P+E  ++ SF
Sbjct: 130 LSKQRNDASAKPSIWVNTLFFCSLYLVAIGQGGHKPCVQAFGADQFDAEDPKEVIARGSF 189

Query: 187 FNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSI--- 246
           FNWWF  + AG  ++I++V Y +EN++W+ GFGIPC+ M++A  +FL G   YRY     
Sbjct: 190 FNWWFLSLSAGISISIIVVAYVQENVNWAFGFGIPCLFMVMALAIFLLGRKIYRYPKGHH 249

Query: 247 -KIYAQTPFLRIGRVFVSAIRNCRAS---STVIFDEEGDGPDLSQQNAGQFRFLNKACIV 306
            ++ +   F RIGRVFV A +N +     S++  D+       S++   +  FL KA I 
Sbjct: 250 EEVNSSNTFARIGRVFVIAFKNRKLRLEHSSLELDQGLLEDGQSEKRKDRLNFLAKAMI- 309

Query: 307 PKDSDKHGVM-CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRS 366
                + GV  CS  +V++AKA++R+ P+WIT +V  I +AQ  TFFTKQG T+DR I  
Sbjct: 310 ----SREGVEPCSGRDVDDAKALVRLIPIWITYVVSTIPYAQYITFFTKQGVTVDRRILP 369

Query: 367 GFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSML 426
           G  IPAA+L SFV +SI+I + +Y+ +F+PIAR  T    GIT LQRIG G+V+S F+M+
Sbjct: 370 GVEIPAASLLSFVGVSILISVPLYERVFLPIARKITKKPFGITMLQRIGAGMVLSVFNMM 429

Query: 427 VAAMVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVP 486
           +AA+VE KRL++A EHGLVD+PD+ +PMS WW VPQY L G+ ++F++VG QEFFYDQVP
Sbjct: 430 LAALVESKRLKIAREHGLVDKPDVTVPMSIWWFVPQYLLLGMIDLFSMVGTQEFFYDQVP 489

Query: 487 TDLKSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSG 546
           T+L+S+GL+   S +G+ S LS  L+S ID  TG   ++ WF++NLN+AH+DYFY+LL+ 
Sbjct: 490 TELRSIGLSLSLSAMGLSSFLSGFLISLIDWATG---KDGWFNSNLNRAHVDYFYWLLAA 549

BLAST of Cucsa.061550 vs. NCBI nr
Match: gi|778708116|ref|XP_011656125.1| (PREDICTED: protein NRT1/ PTR FAMILY 5.10 [Cucumis sativus])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 1

Query: 1   MELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           MELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120
           GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 MLPTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 180
           MLPTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW
Sbjct: 121 MLPTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 180

Query: 181 WFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQT 240
           WFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQT
Sbjct: 181 WFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQT 240

Query: 241 PFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDSDKHGVM 300
           PFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDSDKHGVM
Sbjct: 241 PFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDSDKHGVM 300

Query: 301 CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIPAAALDS 360
           CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIPAAALDS
Sbjct: 301 CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIPAAALDS 360

Query: 361 FVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMVERKRLR 420
           FVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMVERKRLR
Sbjct: 361 FVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMVERKRLR 420

Query: 421 VADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLAFY 480
           VADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLAFY
Sbjct: 421 VADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLAFY 480

Query: 481 TSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVAFVAFLF 540
           TSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVAFVAFLF
Sbjct: 481 TSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVAFVAFLF 540

Query: 541 VSKSH 546
           VSKSH
Sbjct: 541 VSKSH 545

BLAST of Cucsa.061550 vs. NCBI nr
Match: gi|659119482|ref|XP_008459680.1| (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo])

HSP 1 Score: 1035.8 bits (2677), Expect = 2.8e-299
Identity = 520/545 (95.41%), Postives = 531/545 (97.43%), Query Frame = 1

Query: 1   MELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           ME PTPLLSQTVE AVDFKG+P LRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MEQPTPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120
           GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 MLPTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 180
           MLPTTSSQNSQFQL+ FFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW
Sbjct: 121 MLPTTSSQNSQFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 180

Query: 181 WFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQT 240
           WFFG+CAGTFVAILLVTYTEENLSWSLGFGIPCIMM+IASFLFLFGTNTYRYSIK+YA+T
Sbjct: 181 WFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGTNTYRYSIKMYAKT 240

Query: 241 PFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKDSDKHGVM 300
           PFLRIGRVFVSAIRNCRASSTV FDEEGDGPDLS+QN GQF FLNKACIVPKDS+KHGVM
Sbjct: 241 PFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFSFLNKACIVPKDSNKHGVM 300

Query: 301 CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFIIPAAALDS 360
           CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSI SGFIIPAAALDS
Sbjct: 301 CSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSILSGFIIPAAALDS 360

Query: 361 FVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAMVERKRLR 420
           FVPLSIVIFITIYDLLFVPIARAFTG+QSGITTLQRIGTGLVVSA SMLVA MVERKRLR
Sbjct: 361 FVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISMLVATMVERKRLR 420

Query: 421 VADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLAFY 480
           +ADEHGLVDRPDI IPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLAFY
Sbjct: 421 IADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGLAFY 480

Query: 481 TSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVVAFVAFLF 540
           TSVLGMGSILSSLLVS IDEVTGGS+QNSWFSNNLNKAHLDYFY LLS LSVVAFVAFLF
Sbjct: 481 TSVLGMGSILSSLLVSIIDEVTGGSEQNSWFSNNLNKAHLDYFYLLLSALSVVAFVAFLF 540

Query: 541 VSKSH 546
           VSKSH
Sbjct: 541 VSKSH 545

BLAST of Cucsa.061550 vs. NCBI nr
Match: gi|778708112|ref|XP_011656124.1| (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis sativus])

HSP 1 Score: 704.5 bits (1817), Expect = 1.5e-199
Identity = 357/560 (63.75%), Postives = 436/560 (77.86%), Query Frame = 1

Query: 1   MELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           M+  TPLL+  V+GAVD+KG+P+LR  S  WRSASF+IG+EVAERF++YGIG+NLITYLT
Sbjct: 7   MDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFSFYGIGANLITYLT 66

Query: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120
           GPLG SV  AAE VN+W+  SMLLTL GAFLADSFFGRYRTI+F+S  Y+LGL LL  S 
Sbjct: 67  GPLGISVVAAAEIVNVWAATSMLLTLFGAFLADSFFGRYRTIVFASISYILGLGLLVLST 126

Query: 121 MLPTTSSQ------------NSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHP 180
           +LPT SS             + +  L+LFF+SL LIGI QGGH+PCVQAFGADQFD  +P
Sbjct: 127 LLPTPSSSICAILNKFTPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNP 186

Query: 181 QEAKSKSSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTN 240
           QEAK KSSFFNWW+FG C G   AI  V+Y +ENLSW++GFGIPC+ M+    LFL GT 
Sbjct: 187 QEAKFKSSFFNWWYFGACFGIVAAIPTVSYAQENLSWAVGFGIPCVSMLTGFILFLLGTE 246

Query: 241 TYRYS-IKIYAQTPFLRIGRVFVSAIRNCRAS-STVIFDEEGDGPDLSQQNAGQFRFLNK 300
           TYR++ +K   ++PF RIGRVF+++IRN RAS ST+ F +E     LS     QF+FLNK
Sbjct: 247 TYRFNTLKQSDKSPFWRIGRVFLASIRNLRASASTITFQDEQTAKYLSTSQ--QFKFLNK 306

Query: 301 ACI-VPKDSDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDR 360
           ACI VP DS+++ + CS SE+EEAKA+LRI  +W+TV+VF I F+QD+TFFTKQ AT+DR
Sbjct: 307 ACIIVPIDSNQNAMTCSVSELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDR 366

Query: 361 SIRSGFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSA 420
           SI SGFIIPAA+L++ +  +IVIFI +YDLLFVPIA+  TG  SGITTLQRIGTG+V+S 
Sbjct: 367 SIMSGFIIPAASLEALISFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVIST 426

Query: 421 FSMLVAAMVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFY 480
            SM VA++VE+KRL+ A +HGLVD P++ IPM FWWLVPQY L GLA+VFT+VGLQE  Y
Sbjct: 427 ISMAVASLVEKKRLKTALDHGLVDTPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCY 486

Query: 481 DQVPTDLKSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYF 540
           DQVP DLKS+G A + S+LGMG+ILSSLL+S ID  T  +   SWFSNNLNKAHLDYFY 
Sbjct: 487 DQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNKAHLDYFYL 546

Query: 541 LLSGLSVVAFVAFLFVSKSH 546
           LL+ LSV  FVAFLFV+KSH
Sbjct: 547 LLAALSVFGFVAFLFVAKSH 564

BLAST of Cucsa.061550 vs. NCBI nr
Match: gi|645270701|ref|XP_008240575.1| (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Prunus mume])

HSP 1 Score: 704.1 bits (1816), Expect = 1.9e-199
Identity = 342/552 (61.96%), Postives = 429/552 (77.72%), Query Frame = 1

Query: 5   TPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           TPLL   VEGAVD+KGRPV R  SGGWRSA F+IGVEVAERFAYYGI  NLIT+LTGPLG
Sbjct: 13  TPLLDDVVEGAVDYKGRPVHRSRSGGWRSAWFIIGVEVAERFAYYGISCNLITFLTGPLG 72

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLPT 124
           QS ATAAE VNIWSG + LL LLGAF+ADSF GRYRTI+F+S +Y+LGL LL+ SA+LP+
Sbjct: 73  QSTATAAENVNIWSGTASLLPLLGAFVADSFLGRYRTIVFASLLYILGLGLLTLSAVLPS 132

Query: 125 -----------TSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKS 184
                      ++S +SQFQ+L FF SLYL+ + QGGHKPCVQAFGADQFDA  P+E K+
Sbjct: 133 LTGSDCQKINQSTSCSSQFQVLFFFFSLYLVAVAQGGHKPCVQAFGADQFDASDPEECKA 192

Query: 185 KSSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYS 244
           KSSFFNWW+F +C GT   ++L+TY ++NLSW LGFGIPCI M++A F+FL GT TYRYS
Sbjct: 193 KSSFFNWWYFSLCFGTTFTLILLTYIQDNLSWGLGFGIPCIAMVLALFIFLLGTRTYRYS 252

Query: 245 IKIYAQTPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKACIVPKD 304
           IK   ++PF+RIGRVFV+A+RN R + + I  EE     L  +++ QF+FLNKA + P D
Sbjct: 253 IKGDEESPFVRIGRVFVTALRNWRTTPSAITSEEESRGTLPHESSEQFKFLNKALLAPDD 312

Query: 305 SDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRSGFII 364
             ++  +C+ +EVEEAK+VLR+FP+W+T L +A+VFAQ STFFTKQGAT+DR+I  G  I
Sbjct: 313 LKENRKVCTVAEVEEAKSVLRLFPIWVTCLAYAVVFAQYSTFFTKQGATMDRTIAPGLDI 372

Query: 365 PAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSMLVAAM 424
           PAA+L +F+ ++IVIF+ IYD +FVPIAR FT   SGIT LQRIG G+ +S  SM VAA+
Sbjct: 373 PAASLQTFISITIVIFVPIYDRIFVPIARVFTRKPSGITMLQRIGIGMFISIISMAVAAL 432

Query: 425 VERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLK 484
           VE KRL+ A ++GL+D P   +PMS WWLVPQY L GLA+VFT+VGLQEFFYDQVP +LK
Sbjct: 433 VEMKRLKTAKDYGLLDMPSATVPMSIWWLVPQYLLAGLADVFTMVGLQEFFYDQVPNELK 492

Query: 485 SMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLSGLSVV 544
           S+GLA Y S+ G+GS LSS L+S ID+ T  + + SWFS NLN+AHLDYFY+LL G+SVV
Sbjct: 493 SIGLALYLSIFGVGSFLSSFLISAIDDATTWAGETSWFSTNLNRAHLDYFYWLLGGISVV 552

Query: 545 AFVAFLFVSKSH 546
               +L+ +KS+
Sbjct: 553 ELAIYLYFAKSY 564

BLAST of Cucsa.061550 vs. NCBI nr
Match: gi|802712040|ref|XP_012084632.1| (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Jatropha curcas])

HSP 1 Score: 700.3 bits (1806), Expect = 2.8e-198
Identity = 336/557 (60.32%), Postives = 430/557 (77.20%), Query Frame = 1

Query: 2   ELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTG 61
           ++ TPLL  TVE AVD+KGRPV R  SGGWRSASF+IGVE+AERFAYYGI SNLITYLTG
Sbjct: 9   DIQTPLLYDTVEPAVDYKGRPVFRSRSGGWRSASFIIGVEIAERFAYYGIASNLITYLTG 68

Query: 62  PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAM 121
            LGQS ATAAE VN WSG + LL L GAF+ADSF GRYRTI+ +S IY+LGL LL+ SAM
Sbjct: 69  SLGQSTATAAENVNAWSGTASLLPLFGAFVADSFLGRYRTIVVASLIYILGLGLLTLSAM 128

Query: 122 LPTTSSQN-------------SQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHP 181
           L ++ S +             S  Q++LFF SLYL+ +GQGGHKPCVQAFGADQFD   P
Sbjct: 129 LASSHSASDCPSTNIIKSCSPSSCQVVLFFFSLYLVALGQGGHKPCVQAFGADQFDGQDP 188

Query: 182 QEAKSKSSFFNWWFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTN 241
           +E KSKSSFFNWW+F +C GT V++L + Y ++NL+W+LGFGIPCIMM++A F+FL GT 
Sbjct: 189 EECKSKSSFFNWWYFCMCGGTLVSLLFLNYIQDNLNWALGFGIPCIMMVVALFIFLVGTK 248

Query: 242 TYRYSIKIYAQTPFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQFRFLNKAC 301
           TYRYS+K+  ++ FLRIGRVFV+ ++N +   + I  EE     L   ++ QF+FLNKA 
Sbjct: 249 TYRYSVKVEERSAFLRIGRVFVATVKNWQTLPSAIDLEEEARGILPHHSSDQFKFLNKAL 308

Query: 302 IVPKDSDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIR 361
           + P  S + G +CS  EVEEAKAVLR+ P+W T L++A+VFAQ +TFFTKQG T+DRSI 
Sbjct: 309 VAPNGSKEGGKVCSLGEVEEAKAVLRLVPIWFTCLIYAVVFAQSATFFTKQGVTMDRSIS 368

Query: 362 SGFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSM 421
            GF +P A+L SF+ L+IV+F+ IYD +FVPIAR  T   SGIT LQRIGTG+ +SA SM
Sbjct: 369 KGFDVPPASLQSFISLAIVLFVPIYDRVFVPIARTLTRKPSGITMLQRIGTGMFLSALSM 428

Query: 422 LVAAMVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQV 481
            +AA+VE +RL ++ E+GLVD P++ +PMS WWL+PQY LFG+A+VFT+VGLQEFFYDQV
Sbjct: 429 AIAALVEMRRLEISREYGLVDMPNVTVPMSVWWLIPQYVLFGVADVFTMVGLQEFFYDQV 488

Query: 482 PTDLKSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQNSWFSNNLNKAHLDYFYFLLS 541
           P++L+S+GL+ Y  + G+GS +SS LVS I++ TGG   +SWFSNNLN+AHLDYFY+LL+
Sbjct: 489 PSELRSVGLSLYLGIFGVGSFISSFLVSAIEKATGGEGHDSWFSNNLNRAHLDYFYWLLA 548

Query: 542 GLSVVAFVAFLFVSKSH 546
           GLS+V  +AFL+ +K +
Sbjct: 549 GLSIVQLIAFLYFAKRY 565