Cucsa.061520 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.061520
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	scaffold00651 : 51750 .. 53362 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACATGGAAGCTTATAGGTTGTGTTTGTGGGTTTTATCCCCCCAACTCCCAACAGGATATTTTTAGGATATTTGTTTTGGATGTCATCCATTGGCTGCACTGCAAATCTTCTGTTGGCTAAGAGAGAATGGGATTTGCCTCTGCTGGAGCGCAGTTGATTTCCCTTCCACCAACTGGGAGCAGTAGCCGCCGTGTCGGGAAGCCATTTCAACCACCTTACTTCAAATTTGCTTATCCTCTCCCTTCTGTATGTTTATTTCTCCTTTTCTTCTCTACCTTAAATCTATTCTCCAACATGTTTGCTACAATGATGGAATCCTGATTTTCTTAAGGTTAAAGGATTGAGCCTTTTTTTTTTTTTTTAATAGAGGAAATACAGTAATATGGGTGTTTGGAGATGGAAGGGTAAACTGCGTTATAGCGTAGAGGGCCGAATGAAGGATGATGATGATGATGATGCTTGTGAACTAGTGAATGGAATGGAGCTTTCAATTGGGGAACAACCTCATCATTCAATCCCTGCATATCTCTTTAAGGCAGTCAAGAACAATAATGGAACTGCTATTCTTCTCTTGTCCGATCTCTTTGCCTTTCAACATTCTTCCATTAGAGACTTTGCTTATCGTCTTGCTTGCATTGGCTTCAAGTATGCTGCCCAAACATTCACATTCAATTTCACTCTCAATACCAATTTTGTATCAAATAATATGAAAATGTTAAAATAATACATCAAAGTTAACGAAATTTATGTTATAATGAAGAAATGTTCATCGCACTTTTATTTCGAATTTAAGAAATTTCGAATCTATCTGATGGATAAGTATAATTTTTTTTTTTTATATTTGAAAATGTCCACTTTGAAACAGGGTTTAAAAATTATGTATCACGTAACATTTTGCTGCAGAATGCATCTTTTTCCTCTTAACCACGCCACAATAGTTTGCTGAAGTTGAACTGCAGTGTCTTACTTCCAGATTTAGTTCACGAAGATGTATGGGAGAAACCGCCAGCTGAACACTGTGCAAAACAAGAGATGCAGAGGATTGCAGAAGATATAGGAACAGTAACAAAATGGTTGACCGAAGAGTTTTCAGCAGCAGGCCTCTCAATGAAGCTTGGGATCATTGGATTTGGGTACGGGGGAGGCCGAGTAATAGATGTTCTAGCTCGAGACGAGGGTACCTGTTTCTTGATTGGAGCCTCCATCTATGGCACTCAGGTAGATCCATCACTTGCTACCAAAGTCAAGGTTCCTGTGTTGCTCATCTCAGGGGACAATGATCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAAGGGATGGGAGAAGGCTCCAAATTAGTGATATTTGGGGGAAGAGGCCATGGCTTTGTTCACAATCCTGGATCTCCAGAAGAAGATGATGATGCTGAAAAGGCTTTTCTAATGCTTAGAAATTGGCTACATCACGGCTTGGTTGCAATTAATAATTTAGCTAACTGCCATGGGTAATATAGTAATAAATGAAATCAATGATTCTGGTCCCCTTTACTTTGATACCAAAATGCCCAAGGCCAGGGCGATGCCACACTTTTACGCAATTCAGCAATGCGATGCCGTCATTAATAAAC

mRNA sequence

ACATGGAAGCTTATAGGTTGTGTTTGTGGGTTTTATCCCCCCAACTCCCAACAGGATATTTTTAGGATATTTGTTTTGGATGTCATCCATTGGCTGCACTGCAAATCTTCTGTTGGCTAAGAGAGAATGGGATTTGCCTCTGCTGGAGCGCAGTTGATTTCCCTTCCACCAACTGGGAGCAGTAGCCGCCGTGTCGGGAAGCCATTTCAACCACCTTACTTCAAATTTGCTTATCCTCTCCCTTCTAGGAAATACAGTAATATGGGTGTTTGGAGATGGAAGGGTAAACTGCGTTATAGCGTAGAGGGCCGAATGAAGGATGATGATGATGATGATGCTTGTGAACTAGTGAATGGAATGGAGCTTTCAATTGGGGAACAACCTCATCATTCAATCCCTGCATATCTCTTTAAGGCAGTCAAGAACAATAATGGAACTGCTATTCTTCTCTTGTCCGATCTCTTTGCCTTTCAACATTCTTCCATTAGAGACTTTGCTTATCGTCTTGCTTGCATTGGCTTCAAATTTAGTTCACGAAGATGTATGGGAGAAACCGCCAGCTGAACACTGTGCAAAACAAGAGATGCAGAGGATTGCAGAAGATATAGGAACAGTAACAAAATGGTTGACCGAAGAGTTTTCAGCAGCAGGCCTCTCAATGAAGCTTGGGATCATTGGATTTGGGTACGGGGGAGGCCGAGTAATAGATGTTCTAGCTCGAGACGAGGGTACCTGTTTCTTGATTGGAGCCTCCATCTATGGCACTCAGGTAGATCCATCACTTGCTACCAAAGTCAAGGTTCCTGTGTTGCTCATCTCAGGGGACAATGATCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAAGGGATGGGAGAAGGCTCCAAATTAGTGATATTTGGGGGAAGAGGCCATGGCTTTGTTCACAATCCTGGATCTCCAGAAGAAGATGATGATGCTGAAAAGGCTTTTCTAATGCTTAGAAATTGGCTACATCACGGCTTGGTTGCAATTAATAATTTAGCTAACTGCCATGGGTAATATAGTAATAAATGAAATCAATGATTCTGGTCCCCTTTACTTTGATACCAAAATGCCCAAGGCCAGGGCGATGCCACACTTTTACGCAATTCAGCAATGCGATGCCGTCATTAATAAAC

Coding sequence (CDS)

ATGGAATGGAGCTTTCAATTGGGGAACAACCTCATCATTCAATCCCTGCATATCTCTTTAAGGCAGTCAAGAACAATAATGGAACTGCTATTCTTCTCTTGTCCGATCTCTTTGCCTTTCAACATTCTTCCATTAGAGACTTTGCTTATCGTCTTGCTTGCATTGGCTTCAAATTTAGTTCACGAAGATGTATGGGAGAAACCGCCAGCTGAACACTGTGCAAAACAAGAGATGCAGAGGATTGCAGAAGATATAGGAACAGTAACAAAATGGTTGACCGAAGAGTTTTCAGCAGCAGGCCTCTCAATGAAGCTTGGGATCATTGGATTTGGGTACGGGGGAGGCCGAGTAATAGATGTTCTAGCTCGAGACGAGGGTACCTGTTTCTTGATTGGAGCCTCCATCTATGGCACTCAGGTAGATCCATCACTTGCTACCAAAGTCAAGGTTCCTGTGTTGCTCATCTCAGGGGACAATGATCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAAGGGATGGGAGAAGGCTCCAAATTAGTGATATTTGGGGGAAGAGGCCATGGCTTTGTTCACAATCCTGGATCTCCAGAAGAAGATGATGATGCTGAAAAGGCTTTTCTAATGCTTAGAAATTGGCTACATCACGGCTTGGTTGCAATTAATAATTTAGCTAACTGCCATGGGTAA

Protein sequence

MEWSFQLGNNLIIQSLHISLRQSRTIMELLFFSCPISLPFNILPLETLLIVLLALASNLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLVAINNLANCHG*

Homology

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BLAST of Cucsa.061520 vs. TrEMBL
Match: A0A0A0KT04_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.0e-180
Identity = 309/310 (99.68%), Postives = 310/310 (100.00%), Query Frame = 1


Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYSVE 60
           MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYSVE
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYSVE 60

Query: 61  GRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120
           GRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI
Sbjct: 61  GRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120

Query: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180
           RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG
Sbjct: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180

Query: 181 LSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240
           LSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDND
Sbjct: 181 LSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240

Query: 241 PLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           PLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV
Sbjct: 241 PLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300

Query: 301 AINNLANCHG 311
           AINNLANCHG
Sbjct: 301 AINNLANCHG 310

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BLAST of Cucsa.061520 vs. TrEMBL
Match: V4T3Z9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001943mg PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.9e-85
Identity = 156/245 (63.67%), Postives = 193/245 (78.78%), Query Frame = 1


Query: 64  KDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDF 123
           +D  DD+ CELVNG+ELSIGE+   +I AYLFK VKNNNGT +LLLSD+F FQ S+ RDF
Sbjct: 65  EDGLDDETCELVNGVELSIGEEADDAIQAYLFKPVKNNNGTGLLLLSDIFGFQDSATRDF 124

Query: 124 AYRLACIGFNVLLPDLVHEDVW--EKPPA---EHCAKQEMQRIAEDIGTVTKWLTEEFSA 183
           AYR+AC G+NVL+PDL   D W  E+P A   E  AKQ+ +RI++DI T TKW+ +EF A
Sbjct: 125 AYRVACNGYNVLVPDLFRGDPWSKERPKAMFEEWLAKQDFERISKDIYTSTKWMVDEFMA 184

Query: 184 AGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGD 243
           AG+S KLGI+GF +GGGRVIDVLA+D+G CF IG S YGT++D SLA+ V VPVL +SGD
Sbjct: 185 AGISKKLGIVGFCFGGGRVIDVLAQDQGGCFGIGVSFYGTRIDQSLASNVNVPVLFVSGD 244

Query: 244 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHG 303
           NDP+C V+V+KD+EK +G GSK++IF GRGHGF H P SPEED+DAE+AF  +RNWLH G
Sbjct: 245 NDPVCAVNVLKDVEKSIGRGSKVLIFKGRGHGFAHRPSSPEEDEDAEQAFTEMRNWLHDG 304

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BLAST of Cucsa.061520 vs. TrEMBL
Match: A0A061FJU8_THECC (Alpha/beta-Hydrolases superfamily protein isoform 2 (Fragment) OS=Theobroma cacao GN=TCM_036508 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.3e-83
Identity = 155/244 (63.52%), Postives = 193/244 (79.10%), Query Frame = 1


Query: 62  RMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIR 121
           +++DD DD+ACELVNG+ELSIGE  ++ I AYLFKAVKNNNGT +LLLSD+F FQ S+ R
Sbjct: 37  KVEDDIDDEACELVNGVELSIGEGDNN-IQAYLFKAVKNNNGTCVLLLSDIFGFQDSATR 96

Query: 122 DFAYRLACIGFNVLLPDLVHEDVW-EKPPA----EHCAKQEMQRIAEDIGTVTKWLTEEF 181
           DFAY++AC G+NVL+PDL   D W E  P     +  A Q+ +R+A+DI T TKW+ +EF
Sbjct: 97  DFAYQVACHGYNVLVPDLFRGDPWAEGRPRTMLEQWLASQDPERVAKDIATSTKWIVDEF 156

Query: 182 SAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLIS 241
           +AAG+S KLGIIGF +GGGRVIDVLA D+G  F    S YGT++DPS A+KVKVPVL IS
Sbjct: 157 TAAGISKKLGIIGFCFGGGRVIDVLAADQGAYFSTAVSFYGTRMDPSAASKVKVPVLFIS 216

Query: 242 GDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLH 301
           GDNDPLCPVSV+ + EK +G+GS++VIF GRGH F H PGSPEED DAE+AF ++RNWLH
Sbjct: 217 GDNDPLCPVSVLSEFEKSIGQGSRVVIFKGRGHAFAHRPGSPEEDGDAEQAFTLMRNWLH 276

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BLAST of Cucsa.061520 vs. TrEMBL
Match: A0A061FJ42_THECC (Alpha/beta-Hydrolases superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_036508 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.3e-83
Identity = 155/244 (63.52%), Postives = 193/244 (79.10%), Query Frame = 1


Query: 62  RMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIR 121
           +++DD DD+ACELVNG+ELSIGE  ++ I AYLFKAVKNNNGT +LLLSD+F FQ S+ R
Sbjct: 90  KVEDDIDDEACELVNGVELSIGEGDNN-IQAYLFKAVKNNNGTCVLLLSDIFGFQDSATR 149

Query: 122 DFAYRLACIGFNVLLPDLVHEDVW-EKPPA----EHCAKQEMQRIAEDIGTVTKWLTEEF 181
           DFAY++AC G+NVL+PDL   D W E  P     +  A Q+ +R+A+DI T TKW+ +EF
Sbjct: 150 DFAYQVACHGYNVLVPDLFRGDPWAEGRPRTMLEQWLASQDPERVAKDIATSTKWIVDEF 209

Query: 182 SAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLIS 241
           +AAG+S KLGIIGF +GGGRVIDVLA D+G  F    S YGT++DPS A+KVKVPVL IS
Sbjct: 210 TAAGISKKLGIIGFCFGGGRVIDVLAADQGAYFSTAVSFYGTRMDPSAASKVKVPVLFIS 269

Query: 242 GDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLH 301
           GDNDPLCPVSV+ + EK +G+GS++VIF GRGH F H PGSPEED DAE+AF ++RNWLH
Sbjct: 270 GDNDPLCPVSVLSEFEKSIGQGSRVVIFKGRGHAFAHRPGSPEEDGDAEQAFTLMRNWLH 329

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BLAST of Cucsa.061520 vs. TrEMBL
Match: A0A061FKZ6_THECC (Alpha/beta-Hydrolases superfamily protein isoform 3 OS=Theobroma cacao GN=TCM_036508 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.3e-83
Identity = 155/244 (63.52%), Postives = 193/244 (79.10%), Query Frame = 1


Query: 62  RMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIR 121
           +++DD DD+ACELVNG+ELSIGE  ++ I AYLFKAVKNNNGT +LLLSD+F FQ S+ R
Sbjct: 67  KVEDDIDDEACELVNGVELSIGEGDNN-IQAYLFKAVKNNNGTCVLLLSDIFGFQDSATR 126

Query: 122 DFAYRLACIGFNVLLPDLVHEDVW-EKPPA----EHCAKQEMQRIAEDIGTVTKWLTEEF 181
           DFAY++AC G+NVL+PDL   D W E  P     +  A Q+ +R+A+DI T TKW+ +EF
Sbjct: 127 DFAYQVACHGYNVLVPDLFRGDPWAEGRPRTMLEQWLASQDPERVAKDIATSTKWIVDEF 186

Query: 182 SAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLIS 241
           +AAG+S KLGIIGF +GGGRVIDVLA D+G  F    S YGT++DPS A+KVKVPVL IS
Sbjct: 187 TAAGISKKLGIIGFCFGGGRVIDVLAADQGAYFSTAVSFYGTRMDPSAASKVKVPVLFIS 246

Query: 242 GDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLH 301
           GDNDPLCPVSV+ + EK +G+GS++VIF GRGH F H PGSPEED DAE+AF ++RNWLH
Sbjct: 247 GDNDPLCPVSVLSEFEKSIGQGSRVVIFKGRGHAFAHRPGSPEEDGDAEQAFTLMRNWLH 306

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BLAST of Cucsa.061520 vs. TAIR10
Match: AT1G35420.2 (AT1G35420.2 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 275.8 bits (704), Expect = 3.3e-74
Identity = 133/248 (53.63%), Postives = 179/248 (72.18%), Query Frame = 1


Query: 58  SVEGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQH 117
           ++   + DD++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQ 
Sbjct: 73  AINVEVDDDEEEEACELVNGTEVSVD-----GVEGYLLTAVKNNNGTGLLLLSDVFGFQD 132

Query: 118 SSIRDFAYRLACIGFNVLLPDLVHEDVWEK--PPAEH---CAKQEMQRIAEDIGTVTKWL 177
           S+ RDFAYR+AC G+NVL+PDL   D W K  P +E+       +  RI +D  T TKW+
Sbjct: 133 SATRDFAYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWM 192

Query: 178 TEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPV 237
            EEF+AAG+S KLG++GF +GGGRV+DVLA DE   F  G S YGT++D ++A  VKVPV
Sbjct: 193 VEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPV 252

Query: 238 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLR 297
           L I+GD DPLC V  + ++E+ +GEGSK+V++ GRGHGFVH P +PE+D DAE+AF ++R
Sbjct: 253 LFIAGDRDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMR 312

Query: 298 NWLHHGLV 301
           NWLHH L+
Sbjct: 313 NWLHHHLI 315

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BLAST of Cucsa.061520 vs. NCBI nr
Match: gi|449447627|ref|XP_004141569.1| (PREDICTED: uncharacterized protein LOC101210518 [Cucumis sativus])

HSP 1 Score: 640.6 bits (1651), Expect = 1.4e-180
Identity = 309/310 (99.68%), Postives = 310/310 (100.00%), Query Frame = 1


Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYSVE 60
           MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYSVE
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYSVE 60

Query: 61  GRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120
           GRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI
Sbjct: 61  GRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120

Query: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180
           RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG
Sbjct: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180

Query: 181 LSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240
           LSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDND
Sbjct: 181 LSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240

Query: 241 PLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           PLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV
Sbjct: 241 PLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300

Query: 301 AINNLANCHG 311
           AINNLANCHG
Sbjct: 301 AINNLANCHG 310

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BLAST of Cucsa.061520 vs. NCBI nr
Match: gi|659119499|ref|XP_008459689.1| (PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo])

HSP 1 Score: 571.6 bits (1472), Expect = 8.2e-160
Identity = 281/311 (90.35%), Postives = 295/311 (94.86%), Query Frame = 1


Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLR-YSV 60
           MGFASAGAQLISLPPTGSSSRRV KP QPPY +FAYPLPS KY+N G W+WKGKLR YSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  EGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSS 120
           +GR+K DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ SS
Sbjct: 61  KGRVK-DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSS 120

Query: 121 IRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAA 180
           IRDFAYRLAC GFNVLLPDLV+ED WEKPPA+H AKQEMQRIAEDIGTVTKWLT+EFSAA
Sbjct: 121 IRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAA 180

Query: 181 GLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDN 240
           GLS KLGIIGFG+GGGRVIDVLARD+G CFLIGAS+YGT+VDPSLATKVKVPVLLISGD+
Sbjct: 181 GLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDD 240

Query: 241 DPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGL 300
           DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVHNPGSPEEDDDAE+AFLMLRNWLHHGL
Sbjct: 241 DPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGL 300

Query: 301 VAINNLANCHG 311
           VA NNLANCHG
Sbjct: 301 VATNNLANCHG 310

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BLAST of Cucsa.061520 vs. NCBI nr
Match: gi|568838486|ref|XP_006473242.1| (PREDICTED: carboxymethylenebutenolidase homolog [Citrus sinensis])

HSP 1 Score: 325.1 bits (832), Expect = 1.3e-85
Identity = 157/245 (64.08%), Postives = 193/245 (78.78%), Query Frame = 1


Query: 64  KDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDF 123
           +D  DD+ CELVNG+ELSIGE+   +I AYLFK VKNNNGT +LLLSD+F FQ S+ RDF
Sbjct: 65  EDGLDDETCELVNGVELSIGEEADDAIQAYLFKPVKNNNGTGLLLLSDIFGFQDSATRDF 124

Query: 124 AYRLACIGFNVLLPDLVHEDVW--EKPPA---EHCAKQEMQRIAEDIGTVTKWLTEEFSA 183
           AYR+AC G+NVL+PDL   D W  E+P A   E  AKQ+ +RI++DI T TKW+ +EF A
Sbjct: 125 AYRVACNGYNVLVPDLFRGDPWSKERPKAMFEEWIAKQDFERISKDIYTSTKWMVDEFMA 184

Query: 184 AGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGD 243
           AG+S KLGI+GF +GGGRVIDVLA+D+G CF IG S YGT++D SLA+ V VPVL +SGD
Sbjct: 185 AGISKKLGIVGFCFGGGRVIDVLAQDQGGCFGIGVSFYGTRIDQSLASNVNVPVLFVSGD 244

Query: 244 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHG 303
           NDPLCPV+ +KD+EK +G GSK++IF GRGHGF H P SPEED+DAE+AF  +RNWLH G
Sbjct: 245 NDPLCPVNALKDVEKSIGRGSKMLIFKGRGHGFAHRPSSPEEDEDAEQAFTEMRNWLHDG 304

[Close]

BLAST of Cucsa.061520 vs. NCBI nr
Match: gi|567884217|ref|XP_006434667.1| (hypothetical protein CICLE_v10001943mg [Citrus clementina])

HSP 1 Score: 322.0 bits (824), Expect = 1.1e-84
Identity = 156/245 (63.67%), Postives = 193/245 (78.78%), Query Frame = 1


Query: 64  KDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDF 123
           +D  DD+ CELVNG+ELSIGE+   +I AYLFK VKNNNGT +LLLSD+F FQ S+ RDF
Sbjct: 65  EDGLDDETCELVNGVELSIGEEADDAIQAYLFKPVKNNNGTGLLLLSDIFGFQDSATRDF 124

Query: 124 AYRLACIGFNVLLPDLVHEDVW--EKPPA---EHCAKQEMQRIAEDIGTVTKWLTEEFSA 183
           AYR+AC G+NVL+PDL   D W  E+P A   E  AKQ+ +RI++DI T TKW+ +EF A
Sbjct: 125 AYRVACNGYNVLVPDLFRGDPWSKERPKAMFEEWLAKQDFERISKDIYTSTKWMVDEFMA 184

Query: 184 AGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGD 243
           AG+S KLGI+GF +GGGRVIDVLA+D+G CF IG S YGT++D SLA+ V VPVL +SGD
Sbjct: 185 AGISKKLGIVGFCFGGGRVIDVLAQDQGGCFGIGVSFYGTRIDQSLASNVNVPVLFVSGD 244

Query: 244 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHG 303
           NDP+C V+V+KD+EK +G GSK++IF GRGHGF H P SPEED+DAE+AF  +RNWLH G
Sbjct: 245 NDPVCAVNVLKDVEKSIGRGSKVLIFKGRGHGFAHRPSSPEEDEDAEQAFTEMRNWLHDG 304

[Close]

BLAST of Cucsa.061520 vs. NCBI nr
Match: gi|1000959462|ref|XP_015576379.1| (PREDICTED: carboxymethylenebutenolidase homolog [Ricinus communis])

HSP 1 Score: 316.6 bits (810), Expect = 4.8e-83
Identity = 156/247 (63.16%), Postives = 198/247 (80.16%), Query Frame = 1


Query: 62  RMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIR 121
           +++DD +D+ACELVNG+ELS+GE    +I AYLF AVKNNNGT +LLLSD+F F+ SS R
Sbjct: 65  KVEDDINDEACELVNGVELSLGEGVD-TISAYLFTAVKNNNGTGVLLLSDIFGFEDSSTR 124

Query: 122 DFAYRLACIGFNVLLPDLVHEDVWEK--PPA---EHCAKQEMQRIAEDIGTVTKWLTEEF 181
           DFAYRLAC G+NVL+PDL   D W K  P A   +  AKQE QR+A+DI T  KW+ +EF
Sbjct: 125 DFAYRLACNGYNVLVPDLFCGDPWTKDRPKAMLEQWIAKQEPQRVAKDIATAAKWMADEF 184

Query: 182 SAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLIS 241
            AAG+S KLGIIGF +GGGRVI+VL+RD+G CF IG S YGT++DPSLA+++KVPVL IS
Sbjct: 185 LAAGISKKLGIIGFCFGGGRVIEVLSRDQGACFGIGVSFYGTRMDPSLASEIKVPVLFIS 244

Query: 242 GDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLH 301
           G++DPLC V+V+KD+EK +G+ S++VIF GRGHGF H P SPEED DAE+AF+++RNWL+
Sbjct: 245 GNSDPLCSVNVLKDIEKDIGQRSRVVIFQGRGHGFAHRPNSPEEDKDAEQAFVIMRNWLY 304

Query: 302 HGLVAIN 304
            GLV  N
Sbjct: 305 DGLVLEN 310

The following BLAST results are available for this feature:

BLAST of Cucsa.061520 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT04_CUCSA	1.0e-180	99.68	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G652280 PE=4 SV=1	[more]
V4T3Z9_9ROSI	7.9e-85	63.67	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001943mg PE=4 SV=1	[more]
A0A061FJU8_THECC	3.3e-83	63.52	Alpha/beta-Hydrolases superfamily protein isoform 2 (Fragment) OS=Theobroma caca...	[more]
A0A061FJ42_THECC	3.3e-83	63.52	Alpha/beta-Hydrolases superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_03...	[more]
A0A061FKZ6_THECC	3.3e-83	63.52	Alpha/beta-Hydrolases superfamily protein isoform 3 OS=Theobroma cacao GN=TCM_03...	[more]

BLAST of Cucsa.061520 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G35420.2	3.3e-74	53.63	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Cucsa.061520 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449447627\|ref\|XP_004141569.1\|	1.4e-180	99.68	PREDICTED: uncharacterized protein LOC101210518 [Cucumis sativus]	[more]
gi\|659119499\|ref\|XP_008459689.1\|	8.2e-160	90.35	PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo]	[more]
gi\|568838486\|ref\|XP_006473242.1\|	1.3e-85	64.08	PREDICTED: carboxymethylenebutenolidase homolog [Citrus sinensis]	[more]
gi\|567884217\|ref\|XP_006434667.1\|	1.1e-84	63.67	hypothetical protein CICLE_v10001943mg [Citrus clementina]	[more]
gi\|1000959462\|ref\|XP_015576379.1\|	4.8e-83	63.16	PREDICTED: carboxymethylenebutenolidase homolog [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002925	Dienelactn_hydro
IPR002925	Dienelactn_hydro

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity
GO:0016787	hydrolase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.061520.2	Cucsa.061520.2	mRNA
Cucsa.061520.1	Cucsa.061520.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002925	Dienelactone hydrolase	PFAM	PF01738	DLH	coord: 75..217 score: 2.0
None	No IPR available	PANTHER	PTHR17630	DIENELACTONE HYDROLASE	coord: 74..225 score: 2.1
None	No IPR available	PANTHER	PTHR17630:SF45	DIENELACTONE HYDROLASE-LIKE PROTEIN	coord: 74..225 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.061520	Cla003861	Watermelon (97103) v1	cgywmB051
Cucsa.061520	Csa5G652280	Cucumber (Chinese Long) v2	cgycuB047
Cucsa.061520	MELO3C022228	Melon (DHL92) v3.5.1	cgymeB051
Cucsa.061520	ClCG08G000170	Watermelon (Charleston Gray)	cgywcgB052
Cucsa.061520	CSPI05G31520	Wild cucumber (PI 183967)	cgycpiB047
Cucsa.061520	Lsi08G000120	Bottle gourd (USVL1VR-Ls)	cgylsiB051
Cucsa.061520	MELO3C022228.2	Melon (DHL92) v3.6.1	cgymedB050
Cucsa.061520	CsaV3_5G040550	Cucumber (Chinese Long) v3	cgycucB049
Cucsa.061520	Cla97C08G144620	Watermelon (97103) v2	cgywmbB051
Cucsa.061520	Bhi06G000234	Wax gourd	cgywgoB052

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.061520	Cucurbita pepo (Zucchini)	cgycpeB0084
Cucsa.061520	Wax gourd	cgywgoB053
Cucsa.061520	Cucurbita maxima (Rimu)	cgycmaB0087
Cucsa.061520	Cucurbita moschata (Rifu)	cgycmoB0082