Cucsa.058150 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.058150 vs. Swiss-Prot
Match: C7A29_PANGI (Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 1.7e-178
Identity = 299/512 (58.40%), Postives = 387/512 (75.59%), Query Frame = 1

Query: 14  MIISF-VLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEA 73
           +I SF  +V+++LLGW++ +W+W RP+KLEK LR QGF GNSYR+  GD+KE   M +EA
Sbjct: 7   LISSFCAIVVVILLGWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMIVMLKEA 66

Query: 74  MSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQR 133
            SKP+N  + I PR+IP   + I +YGKNSF+W+G  P VHIM+P+ +K V S    FQ+
Sbjct: 67  KSKPINLYDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQK 126

Query: 134 PTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMV 193
           P  NPL KL   G+   EG +W KHRK+I+PAFHL+KLK+M+P  Y S +E+V+KWE MV
Sbjct: 127 PRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMV 186

Query: 194 SEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGW 253
           S +G  ELDV+PYL+ + SDVISRTAFGSSYE+G+KIF+LQ +     IQ+S  IY+PG 
Sbjct: 187 STKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQTIYLPGM 246

Query: 254 RFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGE-AIQTDLLSILIESNLNEIKEHG 313
           RFLPTK N +MKEI+KE+   +  ++N++ K+M+AGE +   DLL IL+ESN  EIK+HG
Sbjct: 247 RFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNSKEIKQHG 306

Query: 314 NNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKK 373
           N    GL++ +VI+ECKLF+  GQETT+ LL+WTM++LS + +WQ+RA++EV   FGN K
Sbjct: 307 NT-NFGLTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNK 366

Query: 374 PDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTD 433
           PD+DGL+ LKVV MI  EVLRLYPP+    RT+ +E KLG ++LPAGV+L LPIIL+  D
Sbjct: 367 PDFDGLNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYD 426

Query: 434 RELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQR 493
           +E+WG DA EFNP RFSEGV KATK    Y PF WGPRICIG NFAM+EAKMA++MILQR
Sbjct: 427 QEIWGDDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQR 486

Query: 494 FSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           FSF LSPSY H P A +T QPQ+GAH+ILH L
Sbjct: 487 FSFVLSPSYAHAPHAIITLQPQYGAHLILHSL 517

BLAST of Cucsa.058150 vs. Swiss-Prot
Match: C7A15_ARATH (Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 7.1e-164
Identity = 269/509 (52.85%), Postives = 379/509 (74.46%), Query Frame = 1

Query: 16  ISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMSK 75
           IS VL ++    W+   W+WF+PK LE  LR+QG AG  Y  L GDLK++  M  EA SK
Sbjct: 10  ISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEARSK 69

Query: 76  PMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQRPTM 135
           P+  ++ I+PRV+P   Q  + YG+  F W G +P + IMDPEQ+K VF+ + DFQ+P  
Sbjct: 70  PLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHT 129

Query: 136 NPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMVSEE 195
            PL  +   GL +++G KW KHR+II+PAFH++K+K+M+P F+ SC E+V +W+ +VS++
Sbjct: 130 FPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDK 189

Query: 196 GS-CELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGWRF 255
           GS CE+DV P L +M +DVISRTAFGSSY++G++IFELQ++L    IQ+    +IPG+ +
Sbjct: 190 GSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGYSY 249

Query: 256 LPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHGNNK 315
           LPTKSN +MK  ++EI  ++ G++N++ ++ +AGEA   DLL IL+ESNL +       +
Sbjct: 250 LPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQ------TE 309

Query: 316 GMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKPDY 375
           G G+S +D+++ECKLFY  GQETT+ LL+WTMV+LS + +WQ RAR+EV ++FG+K+PD 
Sbjct: 310 GNGMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 376 DGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDREL 435
           +GL++LKV+TMI  EVLRLYPPV+   R ++KE KLG+LTLP GV +SLPI+L+Q D EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIEL 429

Query: 436 WGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRFSF 495
           WG DA EFNP+RF +G+SKATK+  ++ PF WGPRICIG NFA++EAKMA+++IL+RFSF
Sbjct: 430 WGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSF 489

Query: 496 QLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           ++SPSY H P   +T  PQ GA +I+HKL
Sbjct: 490 EISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of Cucsa.058150 vs. Swiss-Prot
Match: C72A1_CATRO (Secologanin synthase OS=Catharanthus roseus GN=CYP72A1 PE=2 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 2.3e-162
Identity = 262/511 (51.27%), Postives = 366/511 (71.62%), Query Frame = 1

Query: 15  IISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMS 74
           I + +  L++   W++ DW WF PK++EK LRQQGF GN YR L GD+KES  M +EA+S
Sbjct: 12  IAATIFALVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQEALS 71

Query: 75  KPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQR-- 134
           KPM F+N I PR++P ++ TI  YG+NSF W+G +PR+H+M+PE +K V +  + +Q+  
Sbjct: 72  KPMEFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNF 131

Query: 135 PTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMV 194
              NPL+K    G+ S EG KW KHR+IISPAF L+KLK M+P F    ++M++KWE + 
Sbjct: 132 DVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKWEKIA 191

Query: 195 SEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGW 254
            ++GS E+D+ P    + SDVIS+ AFGS+YE+G KIF L  +L    I     +YIPGW
Sbjct: 192 EKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDVYIPGW 251

Query: 255 RFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHGN 314
            +LPTK N +MKEI+KEI+ ++  ++N++ K++KAGE  + DLL +L+ESN+ EI++ GN
Sbjct: 252 SYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGEPGEDDLLGVLLESNIQEIQKQGN 311

Query: 315 NKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKP 374
            K  G+SI DVI+ECKLFY  GQETT  LL WT ++LS + EWQERAR+EV + FG  KP
Sbjct: 312 KKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKP 371

Query: 375 DYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDR 434
           +++ L+ LK V+MI  EVLRLYPPV    + V+K+TKLG+ T+PAG  + LP +++  ++
Sbjct: 372 EFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVMLPTVMLHREK 431

Query: 435 ELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRF 494
            +WG+DA EFNP RF +GV+ ATKN   Y+PF WGPR+C+G NFA+++AK+ L+MILQRF
Sbjct: 432 SIWGEDAMEFNPMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLGLAMILQRF 491

Query: 495 SFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
            F ++PSY H P   LT QPQ G+H+I  KL
Sbjct: 492 KFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522

BLAST of Cucsa.058150 vs. Swiss-Prot
Match: C7263_MEDTR (11-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula GN=CYP72A63 PE=1 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.9e-162
Identity = 273/518 (52.70%), Postives = 370/518 (71.43%), Query Frame = 1

Query: 10  TPTVMIISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYR--ILHGDLKESAA 69
           T T +IIS + VLL ++ W L + +W +PK+ EKLL+ QGF G  Y   +L    K++  
Sbjct: 8   TGTTVIISVLSVLLAVIPWYLLNKLWLKPKRFEKLLKAQGFQGEPYNLSVLKDKSKQNYM 67

Query: 70  MR--EEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFS 129
           ++  +E  SK +  S   AP +   VHQT++ YG NSF+W GT PRV I DP+Q+K VF+
Sbjct: 68  LKLQQEDKSKSIGLSKEAAPSIFTPVHQTVRKYGNNSFLWEGTTPRVIITDPDQIKDVFN 127

Query: 130 FINDFQRPTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMV 189
            I+DF +P +  + K  + G+  HEG+KW KHRKI +PAFHL+KLK M+P F HSCNEM+
Sbjct: 128 KIDDFPKPKLRSIAKYLSVGILDHEGKKWAKHRKIANPAFHLEKLKVMLPAFSHSCNEMI 187

Query: 190 SKWESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSL 249
           SKW+ ++S +G+CE+DV P LQN   DVISRTAFGSSY +G K+F+L  K G F + +  
Sbjct: 188 SKWKELLSSDGTCEIDVWPSLQNFTCDVISRTAFGSSYAEGTKLFQLLKKQG-FLLMTGR 247

Query: 250 GIYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLN 309
               P W  L T +  KMKEI +EI   + G++ ++EK++K GE    DLL IL++SN  
Sbjct: 248 HTNNPLWGLLATTTKTKMKEIDREIHDSLEGIIEKREKALKNGETTNDDLLGILLQSNHA 307

Query: 310 EIKEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFE 369
           E +  GN+K +G++ QDVIDECKLFY+ GQETT++LL+WTMV+L  Y EWQ RAR+EV +
Sbjct: 308 EKQGQGNSKNIGMTTQDVIDECKLFYLAGQETTSSLLVWTMVLLGRYPEWQARAREEVLQ 367

Query: 370 IFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPI 429
           +FGN+ P+ +GLS+LK+VTMI  EVLRL+PP+  + R + K+ KLGNL LP G  +SLPI
Sbjct: 368 VFGNQNPNNEGLSQLKIVTMILYEVLRLFPPLIYFNRALRKDLKLGNLLLPEGTQISLPI 427

Query: 430 ILIQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMAL 489
           +LI  D +LWG DA EF P RF+EG++KATK   +Y PFGWGPRIC+G NFA++EAK+A+
Sbjct: 428 LLIHQDHDLWGDDAKEFKPERFAEGIAKATKGQVSYFPFGWGPRICLGQNFALLEAKIAV 487

Query: 490 SMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           S++LQ FSF+LSP+Y H P   LT QP++GA IILHKL
Sbjct: 488 SLLLQNFSFELSPNYVHVPTTVLTLQPKNGASIILHKL 524

BLAST of Cucsa.058150 vs. Swiss-Prot
Match: C7A13_ARATH (Cytochrome P450 72A13 OS=Arabidopsis thaliana GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.3e-161
Identity = 267/509 (52.46%), Postives = 376/509 (73.87%), Query Frame = 1

Query: 16  ISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMSK 75
           +S  +V++    W+    +W +PK LE  LR+QG AG  Y  L GDLK + +M  EA SK
Sbjct: 10  VSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEARSK 69

Query: 76  PMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQRPTM 135
           P+N ++ I PR++P   Q ++ +G+  F W G +P + IMDPEQ+K VF+ + DFQ+   
Sbjct: 70  PINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHT 129

Query: 136 NPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMVSE- 195
            PL +L   GL S++G KW KHR+II+PAFHL+K+K+M+P F+ SC+E+V +W+ +V++ 
Sbjct: 130 FPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDK 189

Query: 196 EGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGWRF 255
           + SCE+D+ P+L +M +DVISRTAFGSSY++G++IFELQ++L    IQ+     IPG+R+
Sbjct: 190 QSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGYRY 249

Query: 256 LPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHGNNK 315
            PTK N +MK  ++EI  ++ G++N++ ++ +AGEA   DLL IL+ESNL      G  K
Sbjct: 250 FPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSDDLLGILLESNL------GQTK 309

Query: 316 GMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKPDY 375
           G G+S +++++ECKLFY  GQETT  LL+WTMV+LS + +WQ RAR+EV ++FG+K+PD 
Sbjct: 310 GNGMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 376 DGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDREL 435
           +GL++LKV+TMI  EVLRLYPPV    R ++KE +LG+LTLP GV +SLPI+LIQ DREL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDREL 429

Query: 436 WGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRFSF 495
           WG DA EF P+RF +G+SKATKN  ++ PF WGPRICIG NFA++EAKMA+++IL++FSF
Sbjct: 430 WGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSF 489

Query: 496 QLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           +LSPSY H P   LTT PQ GA +ILHKL
Sbjct: 490 ELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of Cucsa.058150 vs. TrEMBL
Match: W9QZH6_9ROSA (Secologanin synthase OS=Morus notabilis GN=L484_024637 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 3.2e-187
Identity = 303/507 (59.76%), Postives = 400/507 (78.90%), Query Frame = 1

Query: 16  ISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMSK 75
           + F ++L+    W++ +W+W RPK+LE  LR+QG  GNSYR+  GDLKES+ M +EA SK
Sbjct: 11  VVFAILLITTWAWRVLNWVWLRPKRLENCLRKQGLKGNSYRLWSGDLKESSLMIKEASSK 70

Query: 76  PMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQRPTM 135
           P+N S  I  R+ P    T+ +YGK+SFMWIG  PRV I +PE +K +F+ + DF +P  
Sbjct: 71  PINLSEDITARIFPFGLHTVNNYGKDSFMWIGPTPRVSITNPEYIKDIFNKLGDFPKPQS 130

Query: 136 NPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMVSEE 195
            PL+KL   G+ ++EG+KW +HRKII+PAFH++KLK ++P FY SC+EM++KWE +++ E
Sbjct: 131 IPLVKLLATGVATYEGEKWTRHRKIINPAFHMEKLKRLVPAFYLSCSEMLNKWERLLNSE 190

Query: 196 GSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGWRFL 255
           GSCELDV  YLQNM  DVISRTAFGSSYE+G++IF+LQ + G  AI+S   +YIPGWRF+
Sbjct: 191 GSCELDVWSYLQNMTGDVISRTAFGSSYEEGRRIFQLQKEQGELAIKSLQTVYIPGWRFV 250

Query: 256 PTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHGNNKG 315
           PTK N +MK+I  EI  L+  +++++E +M+AGEA   DLL IL+ESNL EI+EHGNNK 
Sbjct: 251 PTKLNRRMKKIYNEIEALVKNIIHKREGAMRAGEAPNDDLLGILMESNLKEIQEHGNNKN 310

Query: 316 MGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKPDYD 375
           MG+SI+DVI+ECKLFYI GQETT+TLL+WTMV+LS +  WQ+RAR+EV +IFGN KP +D
Sbjct: 311 MGISIRDVIEECKLFYIAGQETTSTLLVWTMVLLSRFPSWQDRAREEVLQIFGNNKPGFD 370

Query: 376 GLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDRELW 435
           GL++LKVVTMIF EVLRLYPP+ ++ R V K+T+LGNL+LPAGV +S+P IL+  D ELW
Sbjct: 371 GLTQLKVVTMIFYEVLRLYPPLILFTRKVEKKTQLGNLSLPAGVEVSIPTILVHRDTELW 430

Query: 436 GQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRFSFQ 495
           G+DA +F P RF+EG+SK TK   ++ PFGWGPRIC+G NFAM+EAKMAL++ILQRF+F+
Sbjct: 431 GEDAKDFKPERFAEGMSKVTKGQVSFFPFGWGPRICVGQNFAMVEAKMALALILQRFTFE 490

Query: 496 LSPSYTHTPIAGLTTQPQHGAHIILHK 523
           LSPSY H P+  LT QPQ+GAHIILHK
Sbjct: 491 LSPSYAHAPLTILTLQPQYGAHIILHK 517

BLAST of Cucsa.058150 vs. TrEMBL
Match: H1A979_MEDTR (Cytochrome P450 monooxygenase OS=Medicago truncatula GN=CYP72A59v2 PE=2 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 6.0e-186
Identity = 306/516 (59.30%), Postives = 401/516 (77.71%), Query Frame = 1

Query: 8   ISTPTVMIISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAA 67
           ++T T +I+  ++ L L+  W++ +W+W +PKKLEKLLR+QG  GNSYR+L GD+K+   
Sbjct: 1   MATTTTIILLTLMTLSLIWAWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDIKDLLK 60

Query: 68  MREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFI 127
           +R+EA +KPMN S+ I PRV   VHQ++  +GKNSF+W G  PRV+I DP+Q+K V + I
Sbjct: 61  IRKEATTKPMNLSDDIVPRVYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVLNKI 120

Query: 128 NDFQRPTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSK 187
            DFQ+P  NPL++L  NGL S EG++W KHRKII+PAFHL+KLK M+P F+ SC++M+SK
Sbjct: 121 YDFQKPNANPLVRLLANGLVSLEGEQWSKHRKIINPAFHLEKLKIMLPIFHKSCDDMISK 180

Query: 188 WESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGI 247
           WE M+S +GSCE+DV P+LQN ASDVISR AFGSSYE+GK+IF+LQ++      +  + +
Sbjct: 181 WEKMLSSDGSCEMDVWPFLQNFASDVISRAAFGSSYEEGKRIFQLQTEQAELTTKIMMKV 240

Query: 248 YIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEI 307
           YIPGWRFLPT +  +MKEI ++I   +  ++N +E+++KAGEA + DLL IL+ESN  E+
Sbjct: 241 YIPGWRFLPTATPRRMKEIDRDIKASLTDMINNRERALKAGEATKDDLLGILLESNHKEM 300

Query: 308 KEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIF 367
           +EHGNNK +G+S+ DVI+ECKLFY  GQETT+ LL+WTMV+LS Y +WQ RAR+EV ++F
Sbjct: 301 EEHGNNKDVGMSLDDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVLQVF 360

Query: 368 GNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIIL 427
           GN KPD+DGLS LK+VTMI  EVLRLYPPV   AR V+K+ KLGNL LPAGV + L II+
Sbjct: 361 GNNKPDFDGLSHLKIVTMILYEVLRLYPPVIALARRVHKDVKLGNLALPAGVQVFLSIIM 420

Query: 428 IQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSM 487
           +  D ELWG+DA  FNP RFSEGV KAT    ++ PF  GPRICIG NF+M+EAKMA++M
Sbjct: 421 VHHDIELWGEDAKVFNPERFSEGVLKATNGRNSFFPFAGGPRICIGQNFSMLEAKMAIAM 480

Query: 488 ILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           ILQRFSF+LSPSY H P   +T QPQ+GAHIILHKL
Sbjct: 481 ILQRFSFELSPSYAHAPATVITLQPQYGAHIILHKL 516

BLAST of Cucsa.058150 vs. TrEMBL
Match: Q2MJ18_MEDTR (Cytochrome P450 monooxygenase CYP72A59 OS=Medicago truncatula GN=CYP72A59 PE=2 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 7.9e-186
Identity = 306/516 (59.30%), Postives = 400/516 (77.52%), Query Frame = 1

Query: 8   ISTPTVMIISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAA 67
           ++T T +I+  ++ L L+  W++ +W+W +PKKLEKLLR+QG  GNSYR+L GD+ +   
Sbjct: 1   MATTTTIILFTLMTLSLIWAWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDMNDLLK 60

Query: 68  MREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFI 127
           MR+EA SKPMN S+ I PRV   VHQ++  +GKNSF+W G  PRV+I DP+Q+K V + I
Sbjct: 61  MRKEATSKPMNLSDDIVPRVYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVLNKI 120

Query: 128 NDFQRPTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSK 187
            DFQ+P  NPL++L  NGL S EG++W KHRKII+PAFHL+KLK M+P F+ SC++++SK
Sbjct: 121 YDFQKPNANPLVRLLANGLVSLEGEQWSKHRKIINPAFHLEKLKIMLPIFHKSCDDLISK 180

Query: 188 WESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGI 247
           WE M+S +GSCE+DV P+LQN ASDVISR AFGSSYE+GK+IF+LQ++      +  + +
Sbjct: 181 WEKMLSSDGSCEMDVWPFLQNFASDVISRAAFGSSYEEGKRIFQLQTEQAELTTKIMMKV 240

Query: 248 YIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEI 307
           YIPGWRFLPT +  +MKEI ++I   +  ++N +E+++KAGEA + DLL IL+ESN  E+
Sbjct: 241 YIPGWRFLPTATPRRMKEIDRDIKASLTDMINNRERALKAGEATKDDLLGILLESNHKEM 300

Query: 308 KEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIF 367
           +EHGNNK +G+S+ DVI+ECKLFY  GQETT+ LL+WTMV+LS Y +WQ RAR+EV ++F
Sbjct: 301 EEHGNNKDVGMSLDDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVLQVF 360

Query: 368 GNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIIL 427
           GN KPD+DGLS LK+VTMI  EVLRLYPPV   AR V+K+ KLGNL LPAGV + L II+
Sbjct: 361 GNNKPDFDGLSHLKIVTMILYEVLRLYPPVIALARRVHKDVKLGNLALPAGVQVFLSIIM 420

Query: 428 IQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSM 487
           +  D ELWG+DA  FNP RFSEGV KAT    ++ PF  GPRICIG NF+M+EAKMA++M
Sbjct: 421 VHHDIELWGEDAKVFNPERFSEGVLKATNGRNSFFPFAGGPRICIGQNFSMLEAKMAIAM 480

Query: 488 ILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           ILQRFSF+LSPSY H P   +T QPQ+GAHIILHKL
Sbjct: 481 ILQRFSFELSPSYAHAPATVITLQPQYGAHIILHKL 516

BLAST of Cucsa.058150 vs. TrEMBL
Match: M5WPE9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004271mg PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 3.9e-185
Identity = 304/516 (58.91%), Postives = 404/516 (78.29%), Query Frame = 1

Query: 7   TISTPTVMIISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESA 66
           +++   V+ + +V V++    W++ +W+W RPKKLE+ LRQQG AGNSYR+L GD KES+
Sbjct: 4   SVAAGVVLRVVWVSVIITW-AWRVLNWVWLRPKKLERCLRQQGLAGNSYRLLIGDTKESS 63

Query: 67  AMREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSF 126
            M +EA SKPM+  + +APRV+P VHQ++  YGKNSF+WIG  PRV+I +PE +K +F+ 
Sbjct: 64  MMLKEAKSKPMDLFHDLAPRVLPFVHQSMNTYGKNSFIWIGPTPRVNISNPEDMKDIFAK 123

Query: 127 INDFQRPTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVS 186
             DFQ+P  NPL+KL   GL ++EG+KW KHRKII+PAFH +KLK M+P F+ SC+E++ 
Sbjct: 124 YEDFQKPVKNPLVKLLATGLANYEGEKWAKHRKIINPAFHSEKLKRMLPAFHQSCSELIE 183

Query: 187 KWESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLG 246
           +WES+ S+EGSCELDV PYLQN+ +DVISRTAFGSSYE+G+KIF+L        I++   
Sbjct: 184 EWESLASKEGSCELDVWPYLQNLTADVISRTAFGSSYEEGRKIFQLLKDQAQLTIKAIQS 243

Query: 247 IYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNE 306
           +YIPGWRFLPT+ N +MKEI KEI   ++G++N++E++++  EA + DLL IL++SNL E
Sbjct: 244 VYIPGWRFLPTRMNKRMKEIDKEIKGSLMGIINKREEAIRVREATRDDLLGILMDSNLKE 303

Query: 307 IKEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEI 366
           I+E+GN K +G+SI+DVI+ECKLFY  GQETT+ LL+WT+V+LS   +WQ RAR EV ++
Sbjct: 304 IRENGNTKNVGMSIEDVIEECKLFYFAGQETTSVLLVWTLVLLSQNQDWQARARDEVLQV 363

Query: 367 FGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPII 426
           FG+ KPD+DGLS LKVVTMI  EVLRLYP V + +R ++K T+LG L+LPAGV +SL  +
Sbjct: 364 FGSNKPDFDGLSHLKVVTMILLEVLRLYPAVVVLSRAIHKNTQLGKLSLPAGVEVSLNTL 423

Query: 427 LIQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALS 486
           L+  D+ELWG+DA+EF P RFS GVSKATKN  AY PFG GPRICIG NFAM+EAK+AL+
Sbjct: 424 LVHHDKELWGEDANEFKPERFSSGVSKATKNKFAYFPFGGGPRICIGQNFAMVEAKLALA 483

Query: 487 MILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHK 523
           +ILQ F+F+LSPSY H P A +T QPQ GAHIILHK
Sbjct: 484 LILQHFAFELSPSYAHAPSADITLQPQFGAHIILHK 518

BLAST of Cucsa.058150 vs. TrEMBL
Match: I1MIY4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G244200 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 1.9e-184
Identity = 305/511 (59.69%), Postives = 400/511 (78.28%), Query Frame = 1

Query: 15  IISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMS 74
           I++ +L+L L+  WK+  W+W RPK+LEKLLR+QG  GN Y +  GD KE   MR+EA+S
Sbjct: 8   ILTLILILALICAWKVLIWLWLRPKRLEKLLREQGLQGNPYTLFVGDSKEFLKMRKEALS 67

Query: 75  KPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQRPT 134
           KPMN S+ I PRV      ++  +GKNSF+W+G +PRV I+DPEQ+K VF+ I DF +P 
Sbjct: 68  KPMNLSDDIIPRVSSYEQHSVNKHGKNSFIWLGPIPRVTILDPEQIKDVFNKIYDFPKPN 127

Query: 135 MNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMVSE 194
           MNPL+KL   GL  +EG+KW KHR+II+PAF+L+KLK M+P F+ SCN++VSKWE M+S 
Sbjct: 128 MNPLVKLLATGLAGYEGEKWSKHRRIINPAFNLEKLKIMLPLFFKSCNDLVSKWEGMLSS 187

Query: 195 EGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGWRF 254
           EGSCE+D  P+LQN+ASDVI+R+AFGSSYE+G++IF+LQ +     I+  L I IPGWRF
Sbjct: 188 EGSCEMDAWPFLQNLASDVIARSAFGSSYEEGRRIFQLQREQAELLIKVLLKIQIPGWRF 247

Query: 255 LPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHG--N 314
           LPT ++ +MKEI ++I   +  ++N++EK++KAGEA + DLL IL+ESN  EI+EHG  N
Sbjct: 248 LPTNTHRRMKEIDRDIKASLKDMINKREKALKAGEATKNDLLGILLESNHKEIQEHGNRN 307

Query: 315 NKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKP 374
           NK +G+S+++VI+ECKLFY  GQETT+ LL+WTMV+LS Y +WQ RAR+EVF++FG +KP
Sbjct: 308 NKNVGMSLEEVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVFQVFGYQKP 367

Query: 375 DYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDR 434
           D+DGLSRLK+VTMI  EVLRLYPPV+   R++ K+ KLG LTLPAGV + LP ILI  DR
Sbjct: 368 DFDGLSRLKIVTMILYEVLRLYPPVAGMTRSIEKDVKLGTLTLPAGVHVLLPTILIHHDR 427

Query: 435 ELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRF 494
           + WG+DA +FNP RFSEGV KAT    ++ PFGWGPRICIG NF+++EAKMALSMILQ F
Sbjct: 428 KFWGEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQHF 487

Query: 495 SFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           SF+LSP+Y H P A +T QPQ+GAHIIL K+
Sbjct: 488 SFELSPAYAHAPTALITIQPQYGAHIILRKV 518

BLAST of Cucsa.058150 vs. TAIR10
Match: AT3G14690.1 (AT3G14690.1 cytochrome P450, family 72, subfamily A, polypeptide 15)

HSP 1 Score: 578.6 bits (1490), Expect = 4.0e-165
Identity = 269/509 (52.85%), Postives = 379/509 (74.46%), Query Frame = 1

Query: 16  ISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMSK 75
           IS VL ++    W+   W+WF+PK LE  LR+QG AG  Y  L GDLK++  M  EA SK
Sbjct: 10  ISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEARSK 69

Query: 76  PMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQRPTM 135
           P+  ++ I+PRV+P   Q  + YG+  F W G +P + IMDPEQ+K VF+ + DFQ+P  
Sbjct: 70  PLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHT 129

Query: 136 NPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMVSEE 195
            PL  +   GL +++G KW KHR+II+PAFH++K+K+M+P F+ SC E+V +W+ +VS++
Sbjct: 130 FPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDK 189

Query: 196 GS-CELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGWRF 255
           GS CE+DV P L +M +DVISRTAFGSSY++G++IFELQ++L    IQ+    +IPG+ +
Sbjct: 190 GSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGYSY 249

Query: 256 LPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHGNNK 315
           LPTKSN +MK  ++EI  ++ G++N++ ++ +AGEA   DLL IL+ESNL +       +
Sbjct: 250 LPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQ------TE 309

Query: 316 GMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKPDY 375
           G G+S +D+++ECKLFY  GQETT+ LL+WTMV+LS + +WQ RAR+EV ++FG+K+PD 
Sbjct: 310 GNGMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 376 DGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDREL 435
           +GL++LKV+TMI  EVLRLYPPV+   R ++KE KLG+LTLP GV +SLPI+L+Q D EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIEL 429

Query: 436 WGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRFSF 495
           WG DA EFNP+RF +G+SKATK+  ++ PF WGPRICIG NFA++EAKMA+++IL+RFSF
Sbjct: 430 WGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSF 489

Query: 496 QLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           ++SPSY H P   +T  PQ GA +I+HKL
Sbjct: 490 EISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of Cucsa.058150 vs. TAIR10
Match: AT3G14610.1 (AT3G14610.1 cytochrome P450, family 72, subfamily A, polypeptide 7)

HSP 1 Score: 576.6 bits (1485), Expect = 1.5e-164
Identity = 270/518 (52.12%), Postives = 376/518 (72.59%), Query Frame = 1

Query: 8   ISTPTVMIISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAA 67
           +S   V  +  ++ +++L  W++  W+W +PK LE  L++QG  G  Y  L GD+K +  
Sbjct: 1   MSFSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVD 60

Query: 68  MREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFI 127
           M  EA SKP+N ++ I PR++P   + +  +GK  F+WIG +P + I +PEQ+K VF+ +
Sbjct: 61  MMMEARSKPINVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKV 120

Query: 128 NDFQRPTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSK 187
           NDF++ +  PL++L   GL S++G KW  HR+II+PAFHL+K+K+MIP FYH C+E+V +
Sbjct: 121 NDFEKASTFPLIRLLAGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQ 180

Query: 188 WESMVSE-EGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLG 247
           WE + ++ E   E+DV P+L NM +DVIS TAFGSSY++G++IF+LQ +L     Q+   
Sbjct: 181 WEKLFTDKESPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKK 240

Query: 248 IYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNE 307
            YIPG RF PTKSN +MK I +E+  ++ G+++++EK+ +AGE    DLL IL+ESN  E
Sbjct: 241 SYIPGSRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEPANDDLLGILLESNSEE 300

Query: 308 IKEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEI 367
                 ++G G+S++DV+ ECKLFY  GQETT+ LL+WTMV+LS + +WQ RAR+EV ++
Sbjct: 301 ------SQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQV 360

Query: 368 FG-NKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPI 427
            G N KPD + L+ LKV+TMIFNEVLRLYPPV+   R VNKE KLG LTLPAG+ + LP 
Sbjct: 361 LGENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPT 420

Query: 428 ILIQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMAL 487
           IL+Q D ELWG DA +F P RF +G+SKATKN  ++ PFGWGPRICIG NFAM+EAKMA+
Sbjct: 421 ILVQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAM 480

Query: 488 SMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           ++ILQ+FSF+LSPSY H P   +TT+PQ GAH+ILHKL
Sbjct: 481 ALILQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of Cucsa.058150 vs. TAIR10
Match: AT3G14660.1 (AT3G14660.1 cytochrome P450, family 72, subfamily A, polypeptide 13)

HSP 1 Score: 569.3 bits (1466), Expect = 2.4e-162
Identity = 267/509 (52.46%), Postives = 376/509 (73.87%), Query Frame = 1

Query: 16  ISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMSK 75
           +S  +V++    W+    +W +PK LE  LR+QG AG  Y  L GDLK + +M  EA SK
Sbjct: 10  VSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEARSK 69

Query: 76  PMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQRPTM 135
           P+N ++ I PR++P   Q ++ +G+  F W G +P + IMDPEQ+K VF+ + DFQ+   
Sbjct: 70  PINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHT 129

Query: 136 NPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMVSE- 195
            PL +L   GL S++G KW KHR+II+PAFHL+K+K+M+P F+ SC+E+V +W+ +V++ 
Sbjct: 130 FPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDK 189

Query: 196 EGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGWRF 255
           + SCE+D+ P+L +M +DVISRTAFGSSY++G++IFELQ++L    IQ+     IPG+R+
Sbjct: 190 QSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGYRY 249

Query: 256 LPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHGNNK 315
            PTK N +MK  ++EI  ++ G++N++ ++ +AGEA   DLL IL+ESNL      G  K
Sbjct: 250 FPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSDDLLGILLESNL------GQTK 309

Query: 316 GMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKPDY 375
           G G+S +++++ECKLFY  GQETT  LL+WTMV+LS + +WQ RAR+EV ++FG+K+PD 
Sbjct: 310 GNGMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 376 DGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDREL 435
           +GL++LKV+TMI  EVLRLYPPV    R ++KE +LG+LTLP GV +SLPI+LIQ DREL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDREL 429

Query: 436 WGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRFSF 495
           WG DA EF P+RF +G+SKATKN  ++ PF WGPRICIG NFA++EAKMA+++IL++FSF
Sbjct: 430 WGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSF 489

Query: 496 QLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           +LSPSY H P   LTT PQ GA +ILHKL
Sbjct: 490 ELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of Cucsa.058150 vs. TAIR10
Match: AT3G14650.1 (AT3G14650.1 cytochrome P450, family 72, subfamily A, polypeptide 11)

HSP 1 Score: 568.5 bits (1464), Expect = 4.1e-162
Identity = 265/509 (52.06%), Postives = 375/509 (73.67%), Query Frame = 1

Query: 16  ISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMSK 75
           +S  +V++    W+   W+WF+PK LE  LR+QG AG  Y  L GDLK++ +MR EA SK
Sbjct: 10  VSVAVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEARSK 69

Query: 76  PMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQRPTM 135
           P+N ++ I PR++P   Q ++ +G+  F W G +P + IMDPEQ+  V + + DFQ+   
Sbjct: 70  PINLTDDITPRIVPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYDFQKAHT 129

Query: 136 NPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMVSE- 195
            PL +L   G+ S++G KW KHR+II+PAFHL+K+K+M+P F+ SC+E+V KW+ +VS+ 
Sbjct: 130 FPLGRLIATGVLSYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVSDK 189

Query: 196 EGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGWRF 255
           E SCE+DV P L +M +DVISRTAFGSS  +G++IFELQ++L    IQ+    +IPG+ +
Sbjct: 190 ESSCEVDVWPGLVSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRKAFIPGYSY 249

Query: 256 LPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHGNNK 315
           LPTK N +MK  ++EI  ++ G++N++ ++ +AGEA   DLL IL+ESNL      G  K
Sbjct: 250 LPTKGNRRMKAKAREIQVILRGIVNKRLRAREAGEAPNDDLLGILLESNL------GQTK 309

Query: 316 GMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKPDY 375
           G G+S +D+++ECKLFY  GQETT+ LL+WTMV+LS + +WQ RAR+EV ++FG+K+PD 
Sbjct: 310 GNGMSTEDLMEECKLFYFVGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 376 DGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDREL 435
           +GL++LKV+TMI  EVLRLYPP+   +R ++KE +LG+LTLP GV+++LPI+L+Q D EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPIPQLSRAIHKEMELGDLTLPGGVLINLPILLVQRDTEL 429

Query: 436 WGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRFSF 495
           WG DA EF P+RF +G+SKATKN  ++ PF WG RICIG NFA++EAKMA+++ILQRFSF
Sbjct: 430 WGNDAGEFKPDRFKDGLSKATKNQASFFPFAWGSRICIGQNFALLEAKMAMALILQRFSF 489

Query: 496 QLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           +LSPSY H P    T  PQ GA +I+HKL
Sbjct: 490 ELSPSYVHAPYTVFTIHPQFGAPLIMHKL 512

BLAST of Cucsa.058150 vs. TAIR10
Match: AT3G14680.1 (AT3G14680.1 cytochrome P450, family 72, subfamily A, polypeptide 14)

HSP 1 Score: 565.5 bits (1456), Expect = 3.5e-161
Identity = 264/508 (51.97%), Postives = 373/508 (73.43%), Query Frame = 1

Query: 17  SFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKESAAMREEAMSKP 76
           S  +V++    W+   W+WF PK LE+ LR+QG +G SY  L GD K+  +M  EA SKP
Sbjct: 11  SLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKP 70

Query: 77  MNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQLKTVFSFINDFQRPTMN 136
           +  ++ I PRV+P   Q ++ +G+ +  W G +P + IMDPEQ+K VF+ + DFQ+    
Sbjct: 71  IKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTF 130

Query: 137 PLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHSCNEMVSKWESMVSEEG 196
           PL K+   GL S++G KW +HR+II+PAFHL+K+K+M+  F+ SC+E+V +W+ +VS++G
Sbjct: 131 PLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKG 190

Query: 197 S-CELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFAIQSSLGIYIPGWRFL 256
           S CE+DV P L +M +DVISRTAFGSSY +G +IFELQ++L    +Q+    +IPG+ +L
Sbjct: 191 SSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYL 250

Query: 257 PTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILIESNLNEIKEHGNNKG 316
           PTK N +MK  ++EI  ++ G++N++E++ ++GEA   DLL IL+ESNL      G  +G
Sbjct: 251 PTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNL------GQTEG 310

Query: 317 MGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERARKEVFEIFGNKKPDYD 376
            G+S +D+++ECKLFY+ GQETT+ LL+WTMV+LS + +WQ RAR+EV ++FG+K+PD +
Sbjct: 311 NGMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTE 370

Query: 377 GLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVMLSLPIILIQTDRELW 436
           GL++LKV+TMI  EVLRLYPPV    R ++KE KLG+LTLP GV +SLP++L+  D ELW
Sbjct: 371 GLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELW 430

Query: 437 GQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIEAKMALSMILQRFSFQ 496
           G DA EF P RF +G+SKATKN  ++ PF WGPRICIG NF ++EAKMA+S+ILQRFSF+
Sbjct: 431 GNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFE 490

Query: 497 LSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           LSPSY H P   +T  PQ GAH++LHKL
Sbjct: 491 LSPSYVHAPYTIITLYPQFGAHLMLHKL 512

BLAST of Cucsa.058150 vs. NCBI nr
Match: gi|659103030|ref|XP_008452437.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 963.4 bits (2489), Expect = 1.6e-277
Identity = 477/523 (91.20%), Postives = 499/523 (95.41%), Query Frame = 1

Query: 1   MWYVPDTISTPTVMIISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHG 60
           M +V DTIS PTVMIIS VL LLLLLGWKL DWIWFRPK+LE++LR+QGFAGNSYRILHG
Sbjct: 1   MSFVKDTISVPTVMIISIVL-LLLLLGWKLVDWIWFRPKRLERVLRRQGFAGNSYRILHG 60

Query: 61  DLKESAAMREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQL 120
           DLKESAAMREEAMSKPM FSNHIAPRVIPSV+ TIQ YGKNSFMWIGT+PRVHIMDPEQL
Sbjct: 61  DLKESAAMREEAMSKPMKFSNHIAPRVIPSVYHTIQLYGKNSFMWIGTMPRVHIMDPEQL 120

Query: 121 KTVFSFINDFQRPTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHS 180
           KTVFS INDFQRPTM+PLLKLF NGLFS+EGQKWVKHRKIISPAFHLKKLKDMIPEFYHS
Sbjct: 121 KTVFSLINDFQRPTMSPLLKLFANGLFSYEGQKWVKHRKIISPAFHLKKLKDMIPEFYHS 180

Query: 181 CNEMVSKWESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFA 240
            NEMV KWESM+S EGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQS+LGA  
Sbjct: 181 SNEMVGKWESMISREGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSELGALV 240

Query: 241 IQSSLGIYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILI 300
           IQSSLGIYIPGWRFLPTKSN KMKE+SK+ISTLILGVMNEKEKSMKAGEAIQTDLLSILI
Sbjct: 241 IQSSLGIYIPGWRFLPTKSNRKMKELSKKISTLILGVMNEKEKSMKAGEAIQTDLLSILI 300

Query: 301 ESNLNEIKEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERAR 360
           ESNL+EIK+H NNKGMGLSIQD+IDECKLFYITGQETTATLLIWTMV+LSSYS+WQERAR
Sbjct: 301 ESNLDEIKDHRNNKGMGLSIQDIIDECKLFYITGQETTATLLIWTMVLLSSYSKWQERAR 360

Query: 361 KEVFEIFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVM 420
            EVFEIFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLG LTLPAGVM
Sbjct: 361 AEVFEIFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGKLTLPAGVM 420

Query: 421 LSLPIILIQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIE 480
           LSLPIILIQTD ELWGQDA+EFNP+RFSEGVSKATKNP A+VPFGWGPRICIGLNFAMIE
Sbjct: 421 LSLPIILIQTDPELWGQDAYEFNPDRFSEGVSKATKNPFAFVPFGWGPRICIGLNFAMIE 480

Query: 481 AKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           AKMALSMILQRFSF+LSPSYTHTP  GLTTQPQHGAHIILHKL
Sbjct: 481 AKMALSMILQRFSFELSPSYTHTPHTGLTTQPQHGAHIILHKL 522

BLAST of Cucsa.058150 vs. NCBI nr
Match: gi|778695562|ref|XP_011654016.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 901.4 bits (2328), Expect = 7.6e-259
Identity = 433/523 (82.79%), Postives = 478/523 (91.40%), Query Frame = 1

Query: 1   MWYVPDTISTPTVMIISFVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHG 60
           MWY PDTI       IS VLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHG
Sbjct: 1   MWYAPDTI-------ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHG 60

Query: 61  DLKESAAMREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQL 120
           DLKE AAMREEAMSKPMNFSNHIAPRVIPS++ TIQ YGKNSFMW+G +PRVHIMDPEQL
Sbjct: 61  DLKERAAMREEAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQL 120

Query: 121 KTVFSFINDFQRPTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYHS 180
           KTVFSF NDFQ+PTMNP  KL   G+ + +G+KWVKHRKII+PAFHL+KLKDM+P FYHS
Sbjct: 121 KTVFSFYNDFQKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHS 180

Query: 181 CNEMVSKWESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGAFA 240
           CNEMVSKWESMVS+EGSCELDVMPYL+NMASDVISRTAFGSSYEKGKKIF+LQ++L  F 
Sbjct: 181 CNEMVSKWESMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFV 240

Query: 241 IQSSLGIYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSILI 300
           IQ++LGIYIPGWRFLPTK N KMKEISKEI+TLILG+M+E+EKSMKAGEAIQTDLLSIL+
Sbjct: 241 IQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILM 300

Query: 301 ESNLNEIKEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQERAR 360
           ESN+NEIK+HGN K +G+SI+DVI+ECKLFYI GQETTATLL+WTM++LSSYSEWQERAR
Sbjct: 301 ESNMNEIKQHGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERAR 360

Query: 361 KEVFEIFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAGVM 420
            EVFEIFGNK+P+YDGL+RLKVVTMIFNEVLRLYPP S++ R V KET+LGNLTLP GVM
Sbjct: 361 AEVFEIFGNKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVM 420

Query: 421 LSLPIILIQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAMIE 480
           L LPI+LIQ D ELWG+DAHEFNP RFS GVSKATKNP A++PFGWGPRICIG  FAMIE
Sbjct: 421 LGLPIVLIQRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIE 480

Query: 481 AKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           AKMALSMILQRFSF+LSPSYTH+PIA LTTQPQHGAHIILHKL
Sbjct: 481 AKMALSMILQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 516

BLAST of Cucsa.058150 vs. NCBI nr
Match: gi|778695569|ref|XP_011654017.1| (PREDICTED: cytochrome P450 CYP72A219 isoform X2 [Cucumis sativus])

HSP 1 Score: 894.8 bits (2311), Expect = 7.2e-257
Identity = 436/526 (82.89%), Postives = 478/526 (90.87%), Query Frame = 1

Query: 1   MWYVPDTISTPTVMIISFVLVL-LLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILH 60
           M YV D IST  +++I  +L   LLLLGWKL DWIWFRPKKLEKLLRQQGF GNSYRILH
Sbjct: 1   MAYVLDMISTWLIVVIVVLLSSSLLLLGWKLVDWIWFRPKKLEKLLRQQGFTGNSYRILH 60

Query: 61  GDLKESAAMREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQ 120
           GDLKE AAMR++A+SKPMNFSN+IAPRVIPSVH TIQHYGKNSFMWIG +PRVHIMDPEQ
Sbjct: 61  GDLKERAAMRDQAISKPMNFSNYIAPRVIPSVHHTIQHYGKNSFMWIGPMPRVHIMDPEQ 120

Query: 121 LKTVFSFINDFQRPT--MNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEF 180
           LKTVFS IND+Q+PT  +NPL KL  +GL +HEGQKWVKHRKII+PAFHL+KLKDM+P F
Sbjct: 121 LKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGQKWVKHRKIINPAFHLEKLKDMVPAF 180

Query: 181 YHSCNEMVSKWESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLG 240
           YHSC+EM+SKWESMVS EG CELDVMPYLQNM +DVISRTAFGSSYEKGKKIF+LQ++LG
Sbjct: 181 YHSCDEMISKWESMVSVEGFCELDVMPYLQNMTADVISRTAFGSSYEKGKKIFKLQTELG 240

Query: 241 AFAIQSSLGIYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLS 300
              +QS+LGIYIPGWRFLPTKSNNKMK ISKEISTLI+ ++NE+E SMK+GEAIQTDLLS
Sbjct: 241 EMVMQSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMDIINERETSMKSGEAIQTDLLS 300

Query: 301 ILIESNLNEIKEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQE 360
           IL+ESNLNEIK+HGNNK +G+SI+DVIDECKLFYI GQETTATLL WTMV+LSSYSEWQE
Sbjct: 301 ILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQETTATLLTWTMVLLSSYSEWQE 360

Query: 361 RARKEVFEIFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPA 420
           RAR EVFEIFGNKKPDYDGLSRLKVVTMI NEVLRLYPPVSM+ R V KETKLG LTLPA
Sbjct: 361 RARAEVFEIFGNKKPDYDGLSRLKVVTMILNEVLRLYPPVSMFGRLVKKETKLGKLTLPA 420

Query: 421 GVMLSLPIILIQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFA 480
           GVML LP++L+Q D ELWG+DAHEF P RFSEGVSKA KNP A+VPFGWGPRICIGLNFA
Sbjct: 421 GVMLGLPVVLMQCDPELWGEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFA 480

Query: 481 MIEAKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           MIEAKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL
Sbjct: 481 MIEAKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 526

BLAST of Cucsa.058150 vs. NCBI nr
Match: gi|659103032|ref|XP_008452438.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 887.9 bits (2293), Expect = 8.7e-255
Identity = 434/525 (82.67%), Postives = 477/525 (90.86%), Query Frame = 1

Query: 1   MWYVPDTISTPTVMIISFVLVLLLLL-GWKLADWIWFRPKKLEKLLRQQGFAGNSYRILH 60
           M YVP  I     +IIS+VL+LLLLL GWKL DWIWFRPKKLEK+LR+QGFAGNSYRILH
Sbjct: 1   MSYVPIHIG----VIISYVLLLLLLLLGWKLVDWIWFRPKKLEKILRRQGFAGNSYRILH 60

Query: 61  GDLKESAAMREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQ 120
           GDLKESAAMREEAMSKPMNFSNHIAPRVIPSV+ TIQ YGK S MW+G +PRVHIMDPEQ
Sbjct: 61  GDLKESAAMREEAMSKPMNFSNHIAPRVIPSVYHTIQLYGKKSMMWLGPMPRVHIMDPEQ 120

Query: 121 LKTVFSFINDFQRPTMNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEFYH 180
           LK VFS INDFQ+PTMNPL +L   GL S EGQKWVKHRKII+PAFH++KLKDM+P FYH
Sbjct: 121 LKIVFSSINDFQKPTMNPLTRLLVQGLASLEGQKWVKHRKIINPAFHVEKLKDMVPAFYH 180

Query: 181 SC-NEMVSKWESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLGA 240
           SC NEMVSKWE MVS EGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQ++L  
Sbjct: 181 SCYNEMVSKWEGMVSREGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQTELAN 240

Query: 241 FAIQSSLGIYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLSI 300
             IQ++LGIYIPGWRFLPTKSN KMKEISKEI+TLILG+MNEKEKS+KAGEAIQTDLLSI
Sbjct: 241 LVIQANLGIYIPGWRFLPTKSNRKMKEISKEITTLILGIMNEKEKSIKAGEAIQTDLLSI 300

Query: 301 LIESNLNEIKEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQER 360
           L+ESN+NEIK+HGNNK +G+SI+DVI+ECKLFYI GQETTATLL+WTMV+LSSYSEWQER
Sbjct: 301 LMESNMNEIKQHGNNKNIGMSIEDVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQER 360

Query: 361 ARKEVFEIFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPAG 420
           AR EVFEIFGNKKPDYDGL+RLKVVTMIFNEVLRLYPPV+M+ R + KET+LGNLTLP G
Sbjct: 361 ARAEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVNMFGRIIRKETRLGNLTLPEG 420

Query: 421 VMLSLPIILIQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFAM 480
           VML LP++LI  D ELWG+DAHEFNP RFSEG+SKATKNP A++PFGWGPRICIG NFAM
Sbjct: 421 VMLGLPVVLIHLDPELWGEDAHEFNPERFSEGISKATKNPSAFIPFGWGPRICIGQNFAM 480

Query: 481 IEAKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           IEAKMALS+ILQRFSF+LSPSYTH PIA LTTQPQHGAHIILHKL
Sbjct: 481 IEAKMALSIILQRFSFELSPSYTHAPIAVLTTQPQHGAHIILHKL 521

BLAST of Cucsa.058150 vs. NCBI nr
Match: gi|778695565|ref|XP_004146005.2| (PREDICTED: cytochrome P450 CYP72A219 isoform X1 [Cucumis sativus])

HSP 1 Score: 881.7 bits (2277), Expect = 6.3e-253
Identity = 429/526 (81.56%), Postives = 473/526 (89.92%), Query Frame = 1

Query: 1   MWYVPDTISTPTVMIISFVLVL-LLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILH 60
           M YV D IST  +++I  +L   LLLLGWKL DWIWFRPKKLEKLLRQQGF GNSYRILH
Sbjct: 1   MAYVLDMISTWLIVVIVVLLSSSLLLLGWKLVDWIWFRPKKLEKLLRQQGFTGNSYRILH 60

Query: 61  GDLKESAAMREEAMSKPMNFSNHIAPRVIPSVHQTIQHYGKNSFMWIGTVPRVHIMDPEQ 120
           GDLKE AAMR++A+SKPMNFSN+IAPRVIPSVH TIQHYGKNSFMWIG +PRVHIMDPEQ
Sbjct: 61  GDLKERAAMRDQAISKPMNFSNYIAPRVIPSVHHTIQHYGKNSFMWIGPMPRVHIMDPEQ 120

Query: 121 LKTVFSFINDFQRPT--MNPLLKLFTNGLFSHEGQKWVKHRKIISPAFHLKKLKDMIPEF 180
           LKTVFS IND+Q+PT  +NPL KL  +GL +HEGQKWVKHRKII+PAFHL+KLKDM+P F
Sbjct: 121 LKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGQKWVKHRKIINPAFHLEKLKDMVPAF 180

Query: 181 YHSCNEMVSKWESMVSEEGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSKLG 240
           YHSC+EM+SKWESMVS EG CELDVMPYLQNM +DVISRTAFGSSYEKGKKIF+LQ++LG
Sbjct: 181 YHSCDEMISKWESMVSVEGFCELDVMPYLQNMTADVISRTAFGSSYEKGKKIFKLQTELG 240

Query: 241 AFAIQSSLGIYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKSMKAGEAIQTDLLS 300
              +QS+LGIYIPGWRFLPTKSNNKMK ISKEISTLI+ ++NE+E SMK+GEAIQTDLLS
Sbjct: 241 EMVMQSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMDIINERETSMKSGEAIQTDLLS 300

Query: 301 ILIESNLNEIKEHGNNKGMGLSIQDVIDECKLFYITGQETTATLLIWTMVMLSSYSEWQE 360
           IL+ESNLNEIK+HGNNK +G+SI+DVIDECKLFYI GQETTATLL WTMV+LSSYSEWQE
Sbjct: 301 ILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQETTATLLTWTMVLLSSYSEWQE 360

Query: 361 RARKEVFEIFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGNLTLPA 420
           RAR EVFEIFGNKKPDYDGLSRLKVVTMI NEVLRLYPPVSM+ R V KETKLG LTLPA
Sbjct: 361 RARAEVFEIFGNKKPDYDGLSRLKVVTMILNEVLRLYPPVSMFGRLVKKETKLGKLTLPA 420

Query: 421 GVMLSLPIILIQTDRELWGQDAHEFNPNRFSEGVSKATKNPCAYVPFGWGPRICIGLNFA 480
           GVML LP++L+Q D ELWG+DAHEF P RFSEGVSKA KNP A+VPFGWGPRICIGLNFA
Sbjct: 421 GVMLGLPVVLMQCDPELWGEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFA 480

Query: 481 MIEAKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 524
           MIEAKM LSMILQRFS +LS SYTH PIA +TTQPQHGAHIILHKL
Sbjct: 481 MIEAKMTLSMILQRFSLELSSSYTHAPIAAITTQPQHGAHIILHKL 526