Cucsa.058130 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.058130 vs. Swiss-Prot
Match: C7A29_PANGI (Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 3.9e-186
Identity = 313/512 (61.13%), Postives = 393/512 (76.76%), Query Frame = 1

Query: 14  MIISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEA 73
           +I SF  ++V+++LGW+I +W+W RP+KLEK LR QGF GNSYR+  GD+KE   M KEA
Sbjct: 7   LISSFCAIVVVILLGWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMIVMLKEA 66

Query: 74  MSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQK 133
            SKP+N  + I PR+IP     I  YGKNSF+WLGP P VHIM+P+ IK V S    FQK
Sbjct: 67  KSKPINLYDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQK 126

Query: 134 PTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMV 193
           P  NPLT+LL  G+A+ EG++WAKHRK+INPAFHLEKLK+M+PA Y S +E+V+KWE MV
Sbjct: 127 PRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMV 186

Query: 194 SREGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGW 253
           S +G  ELDV+P L+ + SDVISRTAFGSSYE+G+KIF+LQ E   L+IQAS  IY+PG 
Sbjct: 187 STKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQTIYLPGM 246

Query: 254 SFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGE-AIQTDLLSILMESNMNEIKQHG 313
            FLPTK N++MKEI+KE+   +  I+N+R K+M AGE +   DLL IL+ESN  EIKQHG
Sbjct: 247 RFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNSKEIKQHG 306

Query: 314 NNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKK 373
           N  + G++++EVIEECKLF+ AGQETT+ LLVWTM+LLS + +WQ+RA+ EV   FGN K
Sbjct: 307 NT-NFGLTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNK 366

Query: 374 PNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRD 433
           P++DGLN LKVV MI  EVLRLYPP+    R + +E KLG ++LP GV+L LPI+L+  D
Sbjct: 367 PDFDGLNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYD 426

Query: 434 PELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQR 493
            E+WG+DA EFNPERFSEGV KATK    + PF WGPRICIG NF M+EAKM ++MILQR
Sbjct: 427 QEIWGDDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQR 486

Query: 494 FSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           FSF LSPSY H+P A +T QPQ+GAH+ILH L
Sbjct: 487 FSFVLSPSYAHAPHAIITLQPQYGAHLILHSL 517

BLAST of Cucsa.058130 vs. Swiss-Prot
Match: C7A15_ARATH (Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 5.1e-170
Identity = 287/516 (55.62%), Postives = 386/516 (74.81%), Query Frame = 1

Query: 13  VMIISFVLLLVLLILGWKI---VDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAM 72
           + + S  + +VL ++ W I   + W+WF+PK LE  LRRQG  G  Y  L GDLK++  M
Sbjct: 3   ISVASVTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTM 62

Query: 73  RKEAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFIN 132
             EA SKP+  ++ I+PRV+P      + YG+  F W GP+P + IMDPEQIK VF+ + 
Sbjct: 63  LSEARSKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVY 122

Query: 133 DFQKPTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKW 192
           DFQKP   PL  ++ +G+AN +G+KWAKHR+IINPAFH+EK+K+MVPAF+ SC E+V +W
Sbjct: 123 DFQKPHTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEW 182

Query: 193 ESMVSREGS-CELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGI 252
           + +VS +GS CE+DV P L +M +DVISRTAFGSSY++G++IF+LQ EL  L+IQA    
Sbjct: 183 DQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKA 242

Query: 253 YIPGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEI 312
           +IPG+S+LPTKSNR+MK  ++EI  ++ GI+N+R ++  AGEA   DLL IL+ESN+ + 
Sbjct: 243 FIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQT 302

Query: 313 KQHGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIF 372
           +  GN    GMS E+++EECKLFY AGQETT+ LLVWTMVLLS + +WQ RAR EV ++F
Sbjct: 303 E--GN----GMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVF 362

Query: 373 GNKKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVL 432
           G+K+P+ +GLN+LKV+TMI  EVLRLYPPV+   R + KE KLG+LTLP GV + LPI+L
Sbjct: 363 GDKEPDAEGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILL 422

Query: 433 IQRDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSM 492
           +Q D ELWG DA EFNP+RF +G+SKATK+  +F PF WGPRICIG NF ++EAKM +++
Sbjct: 423 VQHDIELWGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMAL 482

Query: 493 ILQRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           IL+RFSFE+SPSY H+P   +T  PQ GA +I+HKL
Sbjct: 483 ILRRFSFEISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of Cucsa.058130 vs. Swiss-Prot
Match: C72A1_CATRO (Secologanin synthase OS=Catharanthus roseus GN=CYP72A1 PE=2 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.6e-168
Identity = 281/526 (53.42%), Postives = 379/526 (72.05%), Query Frame = 1

Query: 1   MSYVVDTISNRIVMIISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILH 60
           M   +DTI   I   I F L++      W+++DW WF PK++EK LR+QGF GN YR L 
Sbjct: 1   MEMDMDTIRKAIAATI-FALVMAW---AWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLV 60

Query: 61  GDLKESAAMRKEAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQ 120
           GD+KES  M +EA+SKPM F+N I PR++P + HTI  YG+NSF W+G +PR+H+M+PE 
Sbjct: 61  GDVKESGKMHQEALSKPMEFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPEL 120

Query: 121 IKTVFSFINDFQK--PTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAF 180
           IK V +  + +QK     NPL + L+ G+ + EG KW+KHR+II+PAF LEKLK M+PAF
Sbjct: 121 IKEVLTHSSKYQKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAF 180

Query: 181 YHSCNEMVSKWESMVSREGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELG 240
               ++M++KWE +  ++GS E+D+ P    + SDVIS+ AFGS+YE+G KIF+L  EL 
Sbjct: 181 AICYHDMLTKWEKIAEKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELM 240

Query: 241 NLVIQASLGIYIPGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLS 300
           +L I     +YIPGWS+LPTK N++MKEI+KEIT ++  I+N+R K++ AGE  + DLL 
Sbjct: 241 DLTIDCMRDVYIPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGEPGEDDLLG 300

Query: 301 ILMESNMNEIKQHGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQE 360
           +L+ESN+ EI++ GN KD GMSI +VIEECKLFY AGQETT  LL WT +LLS + EWQE
Sbjct: 301 VLLESNIQEIQKQGNKKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQE 360

Query: 361 RARAEVFEIFGNKKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPK 420
           RAR EV + FG  KP ++ LN LK V+MI  EVLRLYPPV    +IV K+TKLG+ T+P 
Sbjct: 361 RAREEVLQAFGKNKPEFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPA 420

Query: 421 GVMLGLPIVLIQRDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFT 480
           G  + LP V++ R+  +WGEDA EFNP RF +GV+ ATKN   ++PF WGPR+C+G NF 
Sbjct: 421 GTQVMLPTVMLHREKSIWGEDAMEFNPMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFA 480

Query: 481 MIEAKMTLSMILQRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           +++AK+ L+MILQRF F+++PSY H+P   LT QPQ G+H+I  KL
Sbjct: 481 LLQAKLGLAMILQRFKFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522

BLAST of Cucsa.058130 vs. Swiss-Prot
Match: C7A14_ARATH (Cytochrome P450 72A14 OS=Arabidopsis thaliana GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 9.9e-166
Identity = 284/514 (55.25%), Postives = 383/514 (74.51%), Query Frame = 1

Query: 13  VMIISFVLLLVLLILG-WKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRK 72
           V  ++F L +V++    W+ + W+WF PK LE+ LRRQG +G SY  L GD K+  +M  
Sbjct: 5   VSSVTFSLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFI 64

Query: 73  EAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDF 132
           EA SKP+  ++ I PRV+P     ++ +G+ +  W GP+P + IMDPEQIK VF+ + DF
Sbjct: 65  EATSKPIKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDF 124

Query: 133 QKPTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWES 192
           QK    PL+++L  G+ + +G+KWA+HR+IINPAFHLEK+K+MV  F+ SC+E+V +W+ 
Sbjct: 125 QKAHTFPLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDK 184

Query: 193 MVSREGS-CELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYI 252
           +VS +GS CE+DV P L +M +DVISRTAFGSSY +G +IF+LQ EL  LV+QA    +I
Sbjct: 185 LVSDKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFI 244

Query: 253 PGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQ 312
           PG+ +LPTK NR+MK  ++EI  ++ GI+N+RE++  +GEA   DLL IL+ESN+ + + 
Sbjct: 245 PGYIYLPTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNLGQTE- 304

Query: 313 HGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGN 372
            GN    GMS E+++EECKLFY+AGQETT+ LLVWTMVLLS + +WQ RAR EV ++FG+
Sbjct: 305 -GN----GMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGD 364

Query: 373 KKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQ 432
           K+P+ +GLN+LKV+TMI  EVLRLYPPV    R + KE KLG+LTLP GV + LP++L+ 
Sbjct: 365 KQPDTEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVH 424

Query: 433 RDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMIL 492
           RD ELWG DA EF PERF +G+SKATKN  +F PF WGPRICIG NFT++EAKM +S+IL
Sbjct: 425 RDTELWGNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLIL 484

Query: 493 QRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           QRFSFELSPSY H+P   +T  PQ GAH++LHKL
Sbjct: 485 QRFSFELSPSYVHAPYTIITLYPQFGAHLMLHKL 512

BLAST of Cucsa.058130 vs. Swiss-Prot
Match: C7A11_ARATH (Cytochrome P450 72A11 OS=Arabidopsis thaliana GN=CYP72A11 PE=2 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 1.1e-164
Identity = 278/497 (55.94%), Postives = 369/497 (74.25%), Query Frame = 1

Query: 29  WKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEAMSKPMNFSNHIAPRV 88
           W+ + W+WF+PK LE  LRRQG  G  Y  L GDLK++ +MR EA SKP+N ++ I PR+
Sbjct: 22  WRTLQWVWFKPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEARSKPINLTDDITPRI 81

Query: 89  IPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQKPTMNPLTRLLVQGIA 148
           +P     ++ +G+  F W G +P + IMDPEQI  V + + DFQK    PL RL+  G+ 
Sbjct: 82  VPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYDFQKAHTFPLGRLIATGVL 141

Query: 149 NLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS-REGSCELDVMPCL 208
           + +G+KWAKHR+IINPAFHLEK+K+MVPAF+ SC+E+V KW+ +VS +E SCE+DV P L
Sbjct: 142 SYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVSDKESSCEVDVWPGL 201

Query: 209 KNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGWSFLPTKSNRKMKEI 268
            +M +DVISRTAFGSS  +G++IF+LQ EL  L+IQ     +IPG+S+LPTK NR+MK  
Sbjct: 202 VSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRKAFIPGYSYLPTKGNRRMKAK 261

Query: 269 SKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQHGNNKDIGMSIEEVIEE 328
           ++EI  ++ GI+N+R ++  AGEA   DLL IL+ESN+ + K  GN    GMS E+++EE
Sbjct: 262 AREIQVILRGIVNKRLRAREAGEAPNDDLLGILLESNLGQTK--GN----GMSTEDLMEE 321

Query: 329 CKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKPNYDGLNRLKVVTMI 388
           CKLFY  GQETT+ LLVWTMVLLS + +WQ RAR EV ++FG+K+P+ +GLN+LKV+TMI
Sbjct: 322 CKLFYFVGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMI 381

Query: 389 FNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDPELWGEDAHEFNPER 448
             EVLRLYPP+    R + KE +LG+LTLP GV++ LPI+L+QRD ELWG DA EF P+R
Sbjct: 382 LYEVLRLYPPIPQLSRAIHKEMELGDLTLPGGVLINLPILLVQRDTELWGNDAGEFKPDR 441

Query: 449 FSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRFSFELSPSYTHSPSA 508
           F +G+SKATKN  +F PF WG RICIG NF ++EAKM +++ILQRFSFELSPSY H+P  
Sbjct: 442 FKDGLSKATKNQASFFPFAWGSRICIGQNFALLEAKMAMALILQRFSFELSPSYVHAPYT 501

Query: 509 SLTTQPQHGAHIILHKL 525
             T  PQ GA +I+HKL
Sbjct: 502 VFTIHPQFGAPLIMHKL 512

BLAST of Cucsa.058130 vs. TrEMBL
Match: V7BTM1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G063300g PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 1.1e-190
Identity = 314/508 (61.81%), Postives = 406/508 (79.92%), Query Frame = 1

Query: 19  VLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEAMSKPM 78
           +++++LL   WKI++W+WF PK+LEKLLR QGF GN Y++L GD KE   MRKEA+SKPM
Sbjct: 11  LIVILLLTWAWKILNWLWFNPKRLEKLLREQGFQGNPYKLLVGDSKEFLKMRKEALSKPM 70

Query: 79  NFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQKPTMNP 138
           + S+ I PRV   + H+++ +GKNSF+WLGP PRV I+DPEQIK +F+ I DF KP +NP
Sbjct: 71  DLSDDIVPRVSSYINHSVKSHGKNSFIWLGPTPRVTILDPEQIKDIFNKIYDFPKPDINP 130

Query: 139 LTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSREGS 198
           L +LL  G+A  EGEKW+KHR++INPAF+LEKLK M+P F+ SCN++V+KWE M+S +GS
Sbjct: 131 LFKLLATGLAGYEGEKWSKHRRLINPAFNLEKLKAMLPLFFESCNDLVNKWERMLSSDGS 190

Query: 199 CELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGWSFLPT 258
           CE+D  P L+NM SDVI+R+AFGSS+E+G++IF+LQ E   L +Q  L + IPGW FLPT
Sbjct: 191 CEIDAWPFLQNMTSDVIARSAFGSSFEEGRRIFQLQREQTKLALQVILKVQIPGWRFLPT 250

Query: 259 KSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQH--GNNKD 318
           KS+R+MKEI ++I   +  ++ +REK++  GEAI  DLL IL+ESN  EI++H  G+NK+
Sbjct: 251 KSHRRMKEIDRDIKASLKDMIYKREKALKKGEAINDDLLGILLESNHKEIQEHAKGDNKN 310

Query: 319 IGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKPNYD 378
           +GMS+E+VIEECKLFY AGQETT+ LLVWTMVLLS Y +WQERAR EV ++FGN+KP++D
Sbjct: 311 VGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQERAREEVLQVFGNQKPSFD 370

Query: 379 GLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDPELW 438
           GLNRLK+VTMI NEVLRLYPP     R+ +++ KLGN+TLP GV + LP VL+  D E W
Sbjct: 371 GLNRLKIVTMILNEVLRLYPPALGMNRLAKRDLKLGNITLPAGVQIFLPTVLVHHDCEFW 430

Query: 439 GEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRFSFE 498
           GEDA +FNPERFSEGV KATK   +F PFGWGPRICIG NF+++EAKM LSMILQ FSFE
Sbjct: 431 GEDAKQFNPERFSEGVLKATKGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQHFSFE 490

Query: 499 LSPSYTHSPSASLTTQPQHGAHIILHKL 525
           LSP+Y H+P+  +T QPQ+GAHIILHK+
Sbjct: 491 LSPAYAHAPAMLITLQPQYGAHIILHKV 518

BLAST of Cucsa.058130 vs. TrEMBL
Match: V7BXP5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G063200g PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.4e-190
Identity = 314/508 (61.81%), Postives = 407/508 (80.12%), Query Frame = 1

Query: 19  VLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEAMSKPM 78
           +++++LLI  WKI++W+WF PK+LEKLLR QGF GN Y++L GD KE   MRKEA+SKPM
Sbjct: 11  LIVILLLIWAWKILNWLWFNPKRLEKLLREQGFQGNPYKLLVGDSKEFLKMRKEALSKPM 70

Query: 79  NFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQKPTMNP 138
           + S+ I PRV   + H+++ +GKNSF+WLGP PRV I+DPEQIK +F+ I DF KP +NP
Sbjct: 71  DLSDDIVPRVSSYINHSVKSHGKNSFIWLGPTPRVTILDPEQIKDIFNKIYDFPKPDINP 130

Query: 139 LTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSREGS 198
           L +LL  G+A+ EGEKW+KHR++INPAF+LEKLK M+P F+ SCN++V+KWE M+S +GS
Sbjct: 131 LLKLLATGLADYEGEKWSKHRRLINPAFNLEKLKAMLPLFFESCNDLVNKWERMLSSDGS 190

Query: 199 CELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGWSFLPT 258
           CE+D  P L+NM SDVI+R+AFGSS+E+G++IF+LQ E   L +Q  L I IPGW FLPT
Sbjct: 191 CEIDAWPFLQNMTSDVIARSAFGSSFEEGRRIFQLQREQAKLALQVILKIQIPGWRFLPT 250

Query: 259 KSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQH--GNNKD 318
           KS+R+MKEI ++I   +  ++ +REK++  GEAI  DLL IL+ESN  EI++H  G+NK+
Sbjct: 251 KSHRRMKEIDRDIKASLKDMIYKREKALKKGEAINDDLLGILLESNHKEIQEHAKGDNKN 310

Query: 319 IGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKPNYD 378
           +GMS+E+VIEECKLFY AGQETT+ LLVWTMVLLS Y +WQERAR EV ++FGN+KP++D
Sbjct: 311 VGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQERAREEVLQVFGNQKPSFD 370

Query: 379 GLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDPELW 438
           GLNRLK+VTMI NEVLRLYPP     R+ +++ KLGN+ LP GV + LP VL+  + E W
Sbjct: 371 GLNRLKIVTMILNEVLRLYPPALGMNRLAKRDLKLGNIILPAGVQIFLPTVLVHHNCEFW 430

Query: 439 GEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRFSFE 498
           GEDA +FNPERFSEGV KATK   +F PFGWGPRICIG NF+++EAKM LSMILQ FSFE
Sbjct: 431 GEDAKQFNPERFSEGVLKATKGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQHFSFE 490

Query: 499 LSPSYTHSPSASLTTQPQHGAHIILHKL 525
           LSP+Y H+P+  +T QPQ+GAHIILHK+
Sbjct: 491 LSPAYAHAPAMLITLQPQYGAHIILHKV 518

BLAST of Cucsa.058130 vs. TrEMBL
Match: W9QZH6_9ROSA (Secologanin synthase OS=Morus notabilis GN=L484_024637 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 1.8e-190
Identity = 318/510 (62.35%), Postives = 399/510 (78.24%), Query Frame = 1

Query: 16  ISFVLLLVLLIL--GWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEA 75
           +S V+  +LLI    W++++W+W RPK+LE  LR+QG  GNSYR+  GDLKES+ M KEA
Sbjct: 8   LSSVVFAILLITTWAWRVLNWVWLRPKRLENCLRKQGLKGNSYRLWSGDLKESSLMIKEA 67

Query: 76  MSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQK 135
            SKP+N S  I  R+ P   HT+  YGK+SFMW+GP PRV I +PE IK +F+ + DF K
Sbjct: 68  SSKPINLSEDITARIFPFGLHTVNNYGKDSFMWIGPTPRVSITNPEYIKDIFNKLGDFPK 127

Query: 136 PTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMV 195
           P   PL +LL  G+A  EGEKW +HRKIINPAFH+EKLK +VPAFY SC+EM++KWE ++
Sbjct: 128 PQSIPLVKLLATGVATYEGEKWTRHRKIINPAFHMEKLKRLVPAFYLSCSEMLNKWERLL 187

Query: 196 SREGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGW 255
           + EGSCELDV   L+NM  DVISRTAFGSSYE+G++IF+LQ E G L I++   +YIPGW
Sbjct: 188 NSEGSCELDVWSYLQNMTGDVISRTAFGSSYEEGRRIFQLQKEQGELAIKSLQTVYIPGW 247

Query: 256 SFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQHGN 315
            F+PTK NR+MK+I  EI  L+  I+++RE +M AGEA   DLL ILMESN+ EI++HGN
Sbjct: 248 RFVPTKLNRRMKKIYNEIEALVKNIIHKREGAMRAGEAPNDDLLGILMESNLKEIQEHGN 307

Query: 316 NKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKP 375
           NK++G+SI +VIEECKLFYIAGQETT+TLLVWTMVLLS +  WQ+RAR EV +IFGN KP
Sbjct: 308 NKNMGISIRDVIEECKLFYIAGQETTSTLLVWTMVLLSRFPSWQDRAREEVLQIFGNNKP 367

Query: 376 NYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDP 435
            +DGL +LKVVTMIF EVLRLYPP+ +F R V K+T+LGNL+LP GV + +P +L+ RD 
Sbjct: 368 GFDGLTQLKVVTMIFYEVLRLYPPLILFTRKVEKKTQLGNLSLPAGVEVSIPTILVHRDT 427

Query: 436 ELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRF 495
           ELWGEDA +F PERF+EG+SK TK   +F PFGWGPRIC+G NF M+EAKM L++ILQRF
Sbjct: 428 ELWGEDAKDFKPERFAEGMSKVTKGQVSFFPFGWGPRICVGQNFAMVEAKMALALILQRF 487

Query: 496 SFELSPSYTHSPSASLTTQPQHGAHIILHK 524
           +FELSPSY H+P   LT QPQ+GAHIILHK
Sbjct: 488 TFELSPSYAHAPLTILTLQPQYGAHIILHK 517

BLAST of Cucsa.058130 vs. TrEMBL
Match: Q2MJ18_MEDTR (Cytochrome P450 monooxygenase CYP72A59 OS=Medicago truncatula GN=CYP72A59 PE=2 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 9.0e-190
Identity = 317/510 (62.16%), Postives = 397/510 (77.84%), Query Frame = 1

Query: 15  IISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEAM 74
           II F L+ + LI  W+I++W+W +PKKLEKLLR QG  GNSYR+L GD+ +   MRKEA 
Sbjct: 7   IILFTLMTLSLIWAWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDMNDLLKMRKEAT 66

Query: 75  SKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQKP 134
           SKPMN S+ I PRV   V+ ++  +GKNSF+W GP PRV+I DP+QIK V + I DFQKP
Sbjct: 67  SKPMNLSDDIVPRVYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVLNKIYDFQKP 126

Query: 135 TMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS 194
             NPL RLL  G+ +LEGE+W+KHRKIINPAFHLEKLK M+P F+ SC++++SKWE M+S
Sbjct: 127 NANPLVRLLANGLVSLEGEQWSKHRKIINPAFHLEKLKIMLPIFHKSCDDLISKWEKMLS 186

Query: 195 REGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGWS 254
            +GSCE+DV P L+N ASDVISR AFGSSYE+GK+IF+LQTE   L  +  + +YIPGW 
Sbjct: 187 SDGSCEMDVWPFLQNFASDVISRAAFGSSYEEGKRIFQLQTEQAELTTKIMMKVYIPGWR 246

Query: 255 FLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQHGNN 314
           FLPT + R+MKEI ++I   +  ++N RE+++ AGEA + DLL IL+ESN  E+++HGNN
Sbjct: 247 FLPTATPRRMKEIDRDIKASLTDMINNRERALKAGEATKDDLLGILLESNHKEMEEHGNN 306

Query: 315 KDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKPN 374
           KD+GMS+++VIEECKLFY AGQETT+ LLVWTMVLLS Y +WQ RAR EV ++FGN KP+
Sbjct: 307 KDVGMSLDDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVLQVFGNNKPD 366

Query: 375 YDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDPE 434
           +DGL+ LK+VTMI  EVLRLYPPV    R V K+ KLGNL LP GV + L I+++  D E
Sbjct: 367 FDGLSHLKIVTMILYEVLRLYPPVIALARRVHKDVKLGNLALPAGVQVFLSIIMVHHDIE 426

Query: 435 LWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRFS 494
           LWGEDA  FNPERFSEGV KAT    +F PF  GPRICIG NF+M+EAKM ++MILQRFS
Sbjct: 427 LWGEDAKVFNPERFSEGVLKATNGRNSFFPFAGGPRICIGQNFSMLEAKMAIAMILQRFS 486

Query: 495 FELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           FELSPSY H+P+  +T QPQ+GAHIILHKL
Sbjct: 487 FELSPSYAHAPATVITLQPQYGAHIILHKL 516

BLAST of Cucsa.058130 vs. TrEMBL
Match: A0A0B2SBC8_GLYSO (Secologanin synthase OS=Glycine soja GN=glysoja_039974 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 1.5e-189
Identity = 318/511 (62.23%), Postives = 402/511 (78.67%), Query Frame = 1

Query: 16  ISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEAMS 75
           I  ++L++ LI  WK+++W+W RPK+LEKLLR QGF GN Y +  GD KE   MRKEA+S
Sbjct: 8   ILLLILILALICSWKVLNWLWLRPKRLEKLLREQGFRGNPYTLFFGDSKEFLKMRKEAVS 67

Query: 76  KPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQKPT 135
           KPM  S+ I PRV   V H++  +GKNSF+W GPMPRV I+DPEQIK VF+   DF KP 
Sbjct: 68  KPMTLSDDIVPRVSAYVQHSVNKHGKNSFIWFGPMPRVTILDPEQIKDVFNKNYDFPKPN 127

Query: 136 MNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSR 195
           +NPL +LL  G+A  EGEKW+KHR+IINPAF+LEKLK M+P F  SCN++VSKWE M+S 
Sbjct: 128 LNPLVKLLATGLAGYEGEKWSKHRRIINPAFNLEKLKIMLPLFLQSCNDLVSKWEGMLSS 187

Query: 196 EGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGWSF 255
           EGSCE+D  P L+N+ SDVI+R+AFGSSYE+G++IF+LQ E   L+++  L I IPGW F
Sbjct: 188 EGSCEMDAWPFLQNLTSDVIARSAFGSSYEEGRRIFQLQREQTELLMKVILKIQIPGWRF 247

Query: 256 LPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQHG--N 315
           LPTK++R+MKEI +EI   +  ++N+REK++ +GEA + DLL IL+ESN  EI++HG  N
Sbjct: 248 LPTKTHRRMKEIDREIKASLKNMINKREKALKSGEATKNDLLGILLESNHKEIQEHGNRN 307

Query: 316 NKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKP 375
           +K++GMS+E+VIEECKLFY AGQETT+ LLVWTMVLLS Y +WQ RAR E F++FG +KP
Sbjct: 308 SKNVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEAFQVFGYQKP 367

Query: 376 NYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDP 435
           ++DGL+RLK+VTMI  EVLRLYPP+    R+V K+ KLGNLTLP GV + LP VLI  D 
Sbjct: 368 DFDGLSRLKIVTMILYEVLRLYPPLIGMNRLVEKDVKLGNLTLPAGVQVFLPTVLIHHDS 427

Query: 436 ELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRF 495
           ELWGEDA +FNPERFSEGV KAT    +F PFGWGPRICIG NF+++EAKM LSMILQ F
Sbjct: 428 ELWGEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQNF 487

Query: 496 SFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
            FELSP+Y H+P+ S+TTQPQ+GAHIIL K+
Sbjct: 488 LFELSPAYAHAPTMSITTQPQYGAHIILRKV 518

BLAST of Cucsa.058130 vs. TAIR10
Match: AT3G14610.1 (AT3G14610.1 cytochrome P450, family 72, subfamily A, polypeptide 7)

HSP 1 Score: 602.8 bits (1553), Expect = 2.0e-172
Identity = 289/508 (56.89%), Postives = 379/508 (74.61%), Query Frame = 1

Query: 19  VLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEAMSKPM 78
           VL+ V+++  W+IV W+W +PK LE  L+RQG TG  Y  L GD+K +  M  EA SKP+
Sbjct: 11  VLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEARSKPI 70

Query: 79  NFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQKPTMNP 138
           N ++ I PR++P     +  +GK  F+W+GP+P + I +PEQIK VF+ +NDF+K +  P
Sbjct: 71  NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDFEKASTFP 130

Query: 139 LTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS-REG 198
           L RLL  G+A+ +G+KWA HR+IINPAFHLEK+K+M+PAFYH C+E+V +WE + + +E 
Sbjct: 131 LIRLLAGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLFTDKES 190

Query: 199 SCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGWSFLP 258
             E+DV P L NM +DVIS TAFGSSY++G++IF+LQ EL  L+ QA    YIPG  F P
Sbjct: 191 PLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKKSYIPGSRFYP 250

Query: 259 TKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQHGNNKDI 318
           TKSNR+MK I +E+  ++ GI+++REK+  AGE    DLL IL+ESN  E    GN    
Sbjct: 251 TKSNRRMKAIDREVDVILRGIVSKREKAREAGEPANDDLLGILLESNSEE--SQGN---- 310

Query: 319 GMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFG-NKKPNYD 378
           GMS+E+V++ECKLFY AGQETT+ LLVWTMVLLS + +WQ RAR EV ++ G N KP+ +
Sbjct: 311 GMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGENNKPDME 370

Query: 379 GLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDPELW 438
            LN LKV+TMIFNEVLRLYPPV+   R+V KE KLG LTLP G+ + LP +L+QRD ELW
Sbjct: 371 SLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILVQRDTELW 430

Query: 439 GEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRFSFE 498
           G+DA +F PERF +G+SKATKN  +F PFGWGPRICIG NF M+EAKM +++ILQ+FSFE
Sbjct: 431 GDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMALILQKFSFE 490

Query: 499 LSPSYTHSPSASLTTQPQHGAHIILHKL 525
           LSPSY H+P   +TT+PQ GAH+ILHKL
Sbjct: 491 LSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of Cucsa.058130 vs. TAIR10
Match: AT3G14690.1 (AT3G14690.1 cytochrome P450, family 72, subfamily A, polypeptide 15)

HSP 1 Score: 599.0 bits (1543), Expect = 2.9e-171
Identity = 287/516 (55.62%), Postives = 386/516 (74.81%), Query Frame = 1

Query: 13  VMIISFVLLLVLLILGWKI---VDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAM 72
           + + S  + +VL ++ W I   + W+WF+PK LE  LRRQG  G  Y  L GDLK++  M
Sbjct: 3   ISVASVTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTM 62

Query: 73  RKEAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFIN 132
             EA SKP+  ++ I+PRV+P      + YG+  F W GP+P + IMDPEQIK VF+ + 
Sbjct: 63  LSEARSKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVY 122

Query: 133 DFQKPTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKW 192
           DFQKP   PL  ++ +G+AN +G+KWAKHR+IINPAFH+EK+K+MVPAF+ SC E+V +W
Sbjct: 123 DFQKPHTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEW 182

Query: 193 ESMVSREGS-CELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGI 252
           + +VS +GS CE+DV P L +M +DVISRTAFGSSY++G++IF+LQ EL  L+IQA    
Sbjct: 183 DQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKA 242

Query: 253 YIPGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEI 312
           +IPG+S+LPTKSNR+MK  ++EI  ++ GI+N+R ++  AGEA   DLL IL+ESN+ + 
Sbjct: 243 FIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQT 302

Query: 313 KQHGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIF 372
           +  GN    GMS E+++EECKLFY AGQETT+ LLVWTMVLLS + +WQ RAR EV ++F
Sbjct: 303 E--GN----GMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVF 362

Query: 373 GNKKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVL 432
           G+K+P+ +GLN+LKV+TMI  EVLRLYPPV+   R + KE KLG+LTLP GV + LPI+L
Sbjct: 363 GDKEPDAEGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILL 422

Query: 433 IQRDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSM 492
           +Q D ELWG DA EFNP+RF +G+SKATK+  +F PF WGPRICIG NF ++EAKM +++
Sbjct: 423 VQHDIELWGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMAL 482

Query: 493 ILQRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           IL+RFSFE+SPSY H+P   +T  PQ GA +I+HKL
Sbjct: 483 ILRRFSFEISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of Cucsa.058130 vs. TAIR10
Match: AT3G14680.1 (AT3G14680.1 cytochrome P450, family 72, subfamily A, polypeptide 14)

HSP 1 Score: 584.7 bits (1506), Expect = 5.6e-167
Identity = 284/514 (55.25%), Postives = 383/514 (74.51%), Query Frame = 1

Query: 13  VMIISFVLLLVLLILG-WKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRK 72
           V  ++F L +V++    W+ + W+WF PK LE+ LRRQG +G SY  L GD K+  +M  
Sbjct: 5   VSSVTFSLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFI 64

Query: 73  EAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDF 132
           EA SKP+  ++ I PRV+P     ++ +G+ +  W GP+P + IMDPEQIK VF+ + DF
Sbjct: 65  EATSKPIKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDF 124

Query: 133 QKPTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWES 192
           QK    PL+++L  G+ + +G+KWA+HR+IINPAFHLEK+K+MV  F+ SC+E+V +W+ 
Sbjct: 125 QKAHTFPLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDK 184

Query: 193 MVSREGS-CELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYI 252
           +VS +GS CE+DV P L +M +DVISRTAFGSSY +G +IF+LQ EL  LV+QA    +I
Sbjct: 185 LVSDKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFI 244

Query: 253 PGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQ 312
           PG+ +LPTK NR+MK  ++EI  ++ GI+N+RE++  +GEA   DLL IL+ESN+ + + 
Sbjct: 245 PGYIYLPTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNLGQTE- 304

Query: 313 HGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGN 372
            GN    GMS E+++EECKLFY+AGQETT+ LLVWTMVLLS + +WQ RAR EV ++FG+
Sbjct: 305 -GN----GMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGD 364

Query: 373 KKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQ 432
           K+P+ +GLN+LKV+TMI  EVLRLYPPV    R + KE KLG+LTLP GV + LP++L+ 
Sbjct: 365 KQPDTEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVH 424

Query: 433 RDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMIL 492
           RD ELWG DA EF PERF +G+SKATKN  +F PF WGPRICIG NFT++EAKM +S+IL
Sbjct: 425 RDTELWGNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLIL 484

Query: 493 QRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           QRFSFELSPSY H+P   +T  PQ GAH++LHKL
Sbjct: 485 QRFSFELSPSYVHAPYTIITLYPQFGAHLMLHKL 512

BLAST of Cucsa.058130 vs. TAIR10
Match: AT3G14630.1 (AT3G14630.1 cytochrome P450, family 72, subfamily A, polypeptide 9)

HSP 1 Score: 584.3 bits (1505), Expect = 7.3e-167
Identity = 284/512 (55.47%), Postives = 373/512 (72.85%), Query Frame = 1

Query: 14  MIISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEA 73
           ++I+ + L+V+L   W+I++W+W +PK LE  LRRQG  G  Y  L GD++ S +M KEA
Sbjct: 3   IVIASLALVVVLWCIWRILEWVWLKPKMLESYLRRQGLVGTRYTPLVGDVRRSFSMLKEA 62

Query: 74  MSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQK 133
            SKPM  ++ +   V+P  +H +  YGK  F W GP+P + IM+P+ IK V++   DF+K
Sbjct: 63  RSKPMKPTDDLISLVMPYSFHMLNTYGKTFFTWSGPIPAITIMNPQLIKEVYNKFYDFEK 122

Query: 134 PTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMV 193
               PLT LL  G+AN +G+KW KHRKIINPAFH EK+K+MVP FY SC E++ +WE +V
Sbjct: 123 THTFPLTSLLTDGLANADGDKWVKHRKIINPAFHFEKIKNMVPTFYKSCIEVMCEWEKLV 182

Query: 194 SREGS-CELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPG 253
           S +GS CELDV P + NM  DVISRTAFGSSY++G++IF LQ EL +L+I A    YIP 
Sbjct: 183 SDKGSSCELDVWPWIVNMTGDVISRTAFGSSYKEGQRIFILQAELAHLIILALGKNYIPA 242

Query: 254 WSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQHG 313
           +   PTK+NR+MK I KEI  ++ GI++ REK+ +AGEA   DLL IL++SN  + K  G
Sbjct: 243 YRHFPTKNNRRMKTIVKEIQVILRGIISHREKARDAGEAPSDDLLGILLKSNSEQSK--G 302

Query: 314 NNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKK 373
           N    G+++EE++EECKLFY AGQETT+ LL WTMVLLS + +WQ RAR EV ++FG+ K
Sbjct: 303 N----GLNMEEIMEECKLFYFAGQETTSVLLAWTMVLLSQHQDWQARAREEVMQVFGHNK 362

Query: 374 PNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRD 433
           P+  G+N+LKV+TMI  EVLRLYPPV    R   KE KLG++TLP G+ + +P++LI RD
Sbjct: 363 PDLQGINQLKVMTMIIYEVLRLYPPVIQMNRATHKEIKLGDMTLPGGIQVHMPVLLIHRD 422

Query: 434 PELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQR 493
            +LWG+DA EF PERF +G++KATKN   F+PFGWGPRICIG NF ++EAKM L++ILQR
Sbjct: 423 TKLWGDDAAEFKPERFKDGIAKATKNQVCFLPFGWGPRICIGQNFALLEAKMALALILQR 482

Query: 494 FSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           FSFELSPSY HSP    T  PQ GAH+ILHKL
Sbjct: 483 FSFELSPSYVHSPYRVFTIHPQCGAHLILHKL 508

BLAST of Cucsa.058130 vs. TAIR10
Match: AT3G14650.1 (AT3G14650.1 cytochrome P450, family 72, subfamily A, polypeptide 11)

HSP 1 Score: 581.3 bits (1497), Expect = 6.2e-166
Identity = 278/497 (55.94%), Postives = 369/497 (74.25%), Query Frame = 1

Query: 29  WKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEAMSKPMNFSNHIAPRV 88
           W+ + W+WF+PK LE  LRRQG  G  Y  L GDLK++ +MR EA SKP+N ++ I PR+
Sbjct: 22  WRTLQWVWFKPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEARSKPINLTDDITPRI 81

Query: 89  IPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQKPTMNPLTRLLVQGIA 148
           +P     ++ +G+  F W G +P + IMDPEQI  V + + DFQK    PL RL+  G+ 
Sbjct: 82  VPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYDFQKAHTFPLGRLIATGVL 141

Query: 149 NLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS-REGSCELDVMPCL 208
           + +G+KWAKHR+IINPAFHLEK+K+MVPAF+ SC+E+V KW+ +VS +E SCE+DV P L
Sbjct: 142 SYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVSDKESSCEVDVWPGL 201

Query: 209 KNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGWSFLPTKSNRKMKEI 268
            +M +DVISRTAFGSS  +G++IF+LQ EL  L+IQ     +IPG+S+LPTK NR+MK  
Sbjct: 202 VSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRKAFIPGYSYLPTKGNRRMKAK 261

Query: 269 SKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQHGNNKDIGMSIEEVIEE 328
           ++EI  ++ GI+N+R ++  AGEA   DLL IL+ESN+ + K  GN    GMS E+++EE
Sbjct: 262 AREIQVILRGIVNKRLRAREAGEAPNDDLLGILLESNLGQTK--GN----GMSTEDLMEE 321

Query: 329 CKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKPNYDGLNRLKVVTMI 388
           CKLFY  GQETT+ LLVWTMVLLS + +WQ RAR EV ++FG+K+P+ +GLN+LKV+TMI
Sbjct: 322 CKLFYFVGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMI 381

Query: 389 FNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDPELWGEDAHEFNPER 448
             EVLRLYPP+    R + KE +LG+LTLP GV++ LPI+L+QRD ELWG DA EF P+R
Sbjct: 382 LYEVLRLYPPIPQLSRAIHKEMELGDLTLPGGVLINLPILLVQRDTELWGNDAGEFKPDR 441

Query: 449 FSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRFSFELSPSYTHSPSA 508
           F +G+SKATKN  +F PF WG RICIG NF ++EAKM +++ILQRFSFELSPSY H+P  
Sbjct: 442 FKDGLSKATKNQASFFPFAWGSRICIGQNFALLEAKMAMALILQRFSFELSPSYVHAPYT 501

Query: 509 SLTTQPQHGAHIILHKL 525
             T  PQ GA +I+HKL
Sbjct: 502 VFTIHPQFGAPLIMHKL 512

BLAST of Cucsa.058130 vs. NCBI nr
Match: gi|659103032|ref|XP_008452438.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 948.7 bits (2451), Expect = 4.2e-273
Identity = 467/525 (88.95%), Postives = 497/525 (94.67%), Query Frame = 1

Query: 1   MSYVVDTISNRIVMIISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILH 60
           MSYV   I     +IIS+VLLL+LL+LGWK+VDWIWFRPKKLEK+LRRQGF GNSYRILH
Sbjct: 1   MSYVPIHIG----VIISYVLLLLLLLLGWKLVDWIWFRPKKLEKILRRQGFAGNSYRILH 60

Query: 61  GDLKESAAMRKEAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQ 120
           GDLKESAAMR+EAMSKPMNFSNHIAPRVIPSVYHTIQ+YGK S MWLGPMPRVHIMDPEQ
Sbjct: 61  GDLKESAAMREEAMSKPMNFSNHIAPRVIPSVYHTIQLYGKKSMMWLGPMPRVHIMDPEQ 120

Query: 121 IKTVFSFINDFQKPTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYH 180
           +K VFS INDFQKPTMNPLTRLLVQG+A+LEG+KW KHRKIINPAFH+EKLKDMVPAFYH
Sbjct: 121 LKIVFSSINDFQKPTMNPLTRLLVQGLASLEGQKWVKHRKIINPAFHVEKLKDMVPAFYH 180

Query: 181 SC-NEMVSKWESMVSREGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGN 240
           SC NEMVSKWE MVSREGSCELDVMP L+NMASDVISRTAFGSSYEKGKKIF+LQTEL N
Sbjct: 181 SCYNEMVSKWEGMVSREGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQTELAN 240

Query: 241 LVIQASLGIYIPGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSI 300
           LVIQA+LGIYIPGW FLPTKSNRKMKEISKEITTLILGIMNE+EKS+ AGEAIQTDLLSI
Sbjct: 241 LVIQANLGIYIPGWRFLPTKSNRKMKEISKEITTLILGIMNEKEKSIKAGEAIQTDLLSI 300

Query: 301 LMESNMNEIKQHGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQER 360
           LMESNMNEIKQHGNNK+IGMSIE+VIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQER
Sbjct: 301 LMESNMNEIKQHGNNKNIGMSIEDVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQER 360

Query: 361 ARAEVFEIFGNKKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKG 420
           ARAEVFEIFGNKKP+YDGLNRLKVVTMIFNEVLRLYPPV++FGRI+RKET+LGNLTLP+G
Sbjct: 361 ARAEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVNMFGRIIRKETRLGNLTLPEG 420

Query: 421 VMLGLPIVLIQRDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTM 480
           VMLGLP+VLI  DPELWGEDAHEFNPERFSEG+SKATKNP AFIPFGWGPRICIG NF M
Sbjct: 421 VMLGLPVVLIHLDPELWGEDAHEFNPERFSEGISKATKNPSAFIPFGWGPRICIGQNFAM 480

Query: 481 IEAKMTLSMILQRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           IEAKM LS+ILQRFSFELSPSYTH+P A LTTQPQHGAHIILHKL
Sbjct: 481 IEAKMALSIILQRFSFELSPSYTHAPIAVLTTQPQHGAHIILHKL 521

BLAST of Cucsa.058130 vs. NCBI nr
Match: gi|778695562|ref|XP_011654016.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 942.2 bits (2434), Expect = 3.9e-271
Identity = 464/510 (90.98%), Postives = 482/510 (94.51%), Query Frame = 1

Query: 15  IISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILHGDLKESAAMRKEAM 74
           IIS VL+L LL+LGWK+ DWIWFRPKKLEKLLR+QGF GNSYRILHGDLKE AAMR+EAM
Sbjct: 8   IISIVLVL-LLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAM 67

Query: 75  SKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQIKTVFSFINDFQKP 134
           SKPMNFSNHIAPRVIPS+YHTIQ YGKNSFMWLGP+PRVHIMDPEQ+KTVFSF NDFQKP
Sbjct: 68  SKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFYNDFQKP 127

Query: 135 TMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS 194
           TMNP T+LLVQGI NL+GEKW KHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS
Sbjct: 128 TMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS 187

Query: 195 REGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNLVIQASLGIYIPGWS 254
           +EGSCELDVMP LKNMASDVISRTAFGSSYEKGKKIFKLQTEL N VIQ +LGIYIPGW 
Sbjct: 188 KEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWR 247

Query: 255 FLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSILMESNMNEIKQHGNN 314
           FLPTK NRKMKEISKEITTLILGIM+EREKSM AGEAIQTDLLSILMESNMNEIKQHGN 
Sbjct: 248 FLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNK 307

Query: 315 KDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKPN 374
           KDIGMSIE+VIEECKLFYIAGQETTATLLVWTM+LLSSYSEWQERARAEVFEIFGNK+PN
Sbjct: 308 KDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPN 367

Query: 375 YDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGVMLGLPIVLIQRDPE 434
           YDGLNRLKVVTMIFNEVLRLYPP S+F RIVRKET+LGNLTLP GVMLGLPIVLIQRDPE
Sbjct: 368 YDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPE 427

Query: 435 LWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMIEAKMTLSMILQRFS 494
           LWGEDAHEFNPERFS GVSKATKNP AFIPFGWGPRICIG  F MIEAKM LSMILQRFS
Sbjct: 428 LWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFS 487

Query: 495 FELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           FELSPSYTHSP ASLTTQPQHGAHIILHKL
Sbjct: 488 FELSPSYTHSPIASLTTQPQHGAHIILHKL 516

BLAST of Cucsa.058130 vs. NCBI nr
Match: gi|778695569|ref|XP_011654017.1| (PREDICTED: cytochrome P450 CYP72A219 isoform X2 [Cucumis sativus])

HSP 1 Score: 903.3 bits (2333), Expect = 2.0e-259
Identity = 437/526 (83.08%), Postives = 483/526 (91.83%), Query Frame = 1

Query: 1   MSYVVDTISNRIVMIISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILH 60
           M+YV+D IS  ++++I  +L   LL+LGWK+VDWIWFRPKKLEKLLR+QGFTGNSYRILH
Sbjct: 1   MAYVLDMISTWLIVVIVVLLSSSLLLLGWKLVDWIWFRPKKLEKLLRQQGFTGNSYRILH 60

Query: 61  GDLKESAAMRKEAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQ 120
           GDLKE AAMR +A+SKPMNFSN+IAPRVIPSV+HTIQ YGKNSFMW+GPMPRVHIMDPEQ
Sbjct: 61  GDLKERAAMRDQAISKPMNFSNYIAPRVIPSVHHTIQHYGKNSFMWIGPMPRVHIMDPEQ 120

Query: 121 IKTVFSFINDFQKPT--MNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAF 180
           +KTVFS IND+QKPT  +NPL +LL  G+ N EG+KW KHRKIINPAFHLEKLKDMVPAF
Sbjct: 121 LKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGQKWVKHRKIINPAFHLEKLKDMVPAF 180

Query: 181 YHSCNEMVSKWESMVSREGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELG 240
           YHSC+EM+SKWESMVS EG CELDVMP L+NM +DVISRTAFGSSYEKGKKIFKLQTELG
Sbjct: 181 YHSCDEMISKWESMVSVEGFCELDVMPYLQNMTADVISRTAFGSSYEKGKKIFKLQTELG 240

Query: 241 NLVIQASLGIYIPGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLS 300
            +V+Q++LGIYIPGW FLPTKSN KMK ISKEI+TLI+ I+NERE SM +GEAIQTDLLS
Sbjct: 241 EMVMQSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMDIINERETSMKSGEAIQTDLLS 300

Query: 301 ILMESNMNEIKQHGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQE 360
           ILMESN+NEIKQHGNNKDIGMSIE+VI+ECKLFYIAGQETTATLL WTMVLLSSYSEWQE
Sbjct: 301 ILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQETTATLLTWTMVLLSSYSEWQE 360

Query: 361 RARAEVFEIFGNKKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPK 420
           RARAEVFEIFGNKKP+YDGL+RLKVVTMI NEVLRLYPPVS+FGR+V+KETKLG LTLP 
Sbjct: 361 RARAEVFEIFGNKKPDYDGLSRLKVVTMILNEVLRLYPPVSMFGRLVKKETKLGKLTLPA 420

Query: 421 GVMLGLPIVLIQRDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFT 480
           GVMLGLP+VL+Q DPELWGEDAHEF PERFSEGVSKA KNP AF+PFGWGPRICIGLNF 
Sbjct: 421 GVMLGLPVVLMQCDPELWGEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFA 480

Query: 481 MIEAKMTLSMILQRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           MIEAKM LSMILQRFSF+LSPSYTH+P A LTTQPQHGAHIILHKL
Sbjct: 481 MIEAKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 526

BLAST of Cucsa.058130 vs. NCBI nr
Match: gi|778695565|ref|XP_004146005.2| (PREDICTED: cytochrome P450 CYP72A219 isoform X1 [Cucumis sativus])

HSP 1 Score: 900.6 bits (2326), Expect = 1.3e-258
Identity = 436/526 (82.89%), Postives = 483/526 (91.83%), Query Frame = 1

Query: 1   MSYVVDTISNRIVMIISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILH 60
           M+YV+D IS  ++++I  +L   LL+LGWK+VDWIWFRPKKLEKLLR+QGFTGNSYRILH
Sbjct: 1   MAYVLDMISTWLIVVIVVLLSSSLLLLGWKLVDWIWFRPKKLEKLLRQQGFTGNSYRILH 60

Query: 61  GDLKESAAMRKEAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQ 120
           GDLKE AAMR +A+SKPMNFSN+IAPRVIPSV+HTIQ YGKNSFMW+GPMPRVHIMDPEQ
Sbjct: 61  GDLKERAAMRDQAISKPMNFSNYIAPRVIPSVHHTIQHYGKNSFMWIGPMPRVHIMDPEQ 120

Query: 121 IKTVFSFINDFQKPT--MNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAF 180
           +KTVFS IND+QKPT  +NPL +LL  G+ N EG+KW KHRKIINPAFHLEKLKDMVPAF
Sbjct: 121 LKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGQKWVKHRKIINPAFHLEKLKDMVPAF 180

Query: 181 YHSCNEMVSKWESMVSREGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELG 240
           YHSC+EM+SKWESMVS EG CELDVMP L+NM +DVISRTAFGSSYEKGKKIFKLQTELG
Sbjct: 181 YHSCDEMISKWESMVSVEGFCELDVMPYLQNMTADVISRTAFGSSYEKGKKIFKLQTELG 240

Query: 241 NLVIQASLGIYIPGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLS 300
            +V+Q++LGIYIPGW FLPTKSN KMK ISKEI+TLI+ I+NERE SM +GEAIQTDLLS
Sbjct: 241 EMVMQSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMDIINERETSMKSGEAIQTDLLS 300

Query: 301 ILMESNMNEIKQHGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQE 360
           ILMESN+NEIKQHGNNKDIGMSIE+VI+ECKLFYIAGQETTATLL WTMVLLSSYSEWQE
Sbjct: 301 ILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQETTATLLTWTMVLLSSYSEWQE 360

Query: 361 RARAEVFEIFGNKKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPK 420
           RARAEVFEIFGNKKP+YDGL+RLKVVTMI NEVLRLYPPVS+FGR+V+KETKLG LTLP 
Sbjct: 361 RARAEVFEIFGNKKPDYDGLSRLKVVTMILNEVLRLYPPVSMFGRLVKKETKLGKLTLPA 420

Query: 421 GVMLGLPIVLIQRDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFT 480
           GVMLGLP+VL+Q DPELWGEDAHEF PERFSEGVSKA KNP AF+PFGWGPRICIGLNF 
Sbjct: 421 GVMLGLPVVLMQCDPELWGEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFA 480

Query: 481 MIEAKMTLSMILQRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           MIEAKMTLSMILQRFS ELS SYTH+P A++TTQPQHGAHIILHKL
Sbjct: 481 MIEAKMTLSMILQRFSLELSSSYTHAPIAAITTQPQHGAHIILHKL 526

BLAST of Cucsa.058130 vs. NCBI nr
Match: gi|778695577|ref|XP_004146006.2| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 900.2 bits (2325), Expect = 1.7e-258
Identity = 439/524 (83.78%), Postives = 477/524 (91.03%), Query Frame = 1

Query: 1   MSYVVDTISNRIVMIISFVLLLVLLILGWKIVDWIWFRPKKLEKLLRRQGFTGNSYRILH 60
           M YV   IS  IV+I S VLLL LL LGWK+ DWIWF P++LE+LLRRQGFTGNSY+ILH
Sbjct: 1   MWYVPHMISTPIVIITSLVLLL-LLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILH 60

Query: 61  GDLKESAAMRKEAMSKPMNFSNHIAPRVIPSVYHTIQIYGKNSFMWLGPMPRVHIMDPEQ 120
           GD+KESAAMRKEA+SKPM FSNHIAPRVIPSVYHTIQ YGKNS MWLGP+PRVHIMDPEQ
Sbjct: 61  GDMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQ 120

Query: 121 IKTVFSFINDFQKPTMNPLTRLLVQGIANLEGEKWAKHRKIINPAFHLEKLKDMVPAFYH 180
           +KTVFSF NDFQ+PT +P  RLL+ G+ +LEGEKW KHRKIINPAFHLEKLK MVPAFYH
Sbjct: 121 LKTVFSFFNDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYH 180

Query: 181 SCNEMVSKWESMVSREGSCELDVMPCLKNMASDVISRTAFGSSYEKGKKIFKLQTELGNL 240
           SCNEMVSKWESMVSREGSCELDVMP L+NMA+DVISRTAFGSSYE+GKKIFKLQTEL NL
Sbjct: 181 SCNEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNL 240

Query: 241 VIQASLGIYIPGWSFLPTKSNRKMKEISKEITTLILGIMNEREKSMNAGEAIQTDLLSIL 300
           VIQASL  YIPGW FLPTKSNRK+KE ++EITTL+LGI+NE+EKSM AGE IQTDLLSIL
Sbjct: 241 VIQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSIL 300

Query: 301 MESNMNEIKQHGNNKDIGMSIEEVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERA 360
           MESNMNEIK   N+KD+GMSI+ VIEEC+LFYIAGQETTATLL+WTMVLLSSYS+WQERA
Sbjct: 301 MESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERA 360

Query: 361 RAEVFEIFGNKKPNYDGLNRLKVVTMIFNEVLRLYPPVSIFGRIVRKETKLGNLTLPKGV 420
           RAEVFEIFGNKKP+YDGLNRLKVVTMIFNEVLRLYPPVS+ GR  RKETKLGN+TLP G+
Sbjct: 361 RAEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGI 420

Query: 421 MLGLPIVLIQRDPELWGEDAHEFNPERFSEGVSKATKNPCAFIPFGWGPRICIGLNFTMI 480
           ML LPI+LIQRDPELWGEDAHEFNPERFSEGV KATKNP AF+PFGWGPRICIG NF MI
Sbjct: 421 MLSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMI 480

Query: 481 EAKMTLSMILQRFSFELSPSYTHSPSASLTTQPQHGAHIILHKL 525
           EAKM LSMILQ FSFELSPSYTH+P A+LTTQPQHGAHIILHKL
Sbjct: 481 EAKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 523