Cucsa.058110 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.058110 vs. Swiss-Prot
Match: C7A29_PANGI (Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 8.1e-176
Identity = 295/512 (57.62%), Postives = 379/512 (74.02%), Query Frame = 1

Query: 13  VIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEA 72
           +I +   +++++ LGW++ +W+W  PR+LE+ LR QGF GNSY++  GD+KE   M KEA
Sbjct: 7   LISSFCAIVVVILLGWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMIVMLKEA 66

Query: 73  LSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQR 132
            SKP+   + I PR+IP     I  YGKNS +WLGP P VHIM+P+ +K V S F  FQ+
Sbjct: 67  KSKPINLYDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQK 126

Query: 133 PTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMV 192
           P  +P  +LL  G+   EG++W KHRK+INPAFHLEKLK M+PA Y S +E+V+KWE MV
Sbjct: 127 PRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMV 186

Query: 193 SREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGW 252
           S +G  ELDV+PYL+ + +DVISRTAFGSSYE G+KIF+LQ E   L+IQAS   Y+PG 
Sbjct: 187 STKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQTIYLPGM 246

Query: 253 RFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGE-EIQTDLLSILMESNMNEIKHGR 312
           RFLPTK N+++KE  +E+   +  IIN++ K+M+AGE     DLL IL+ESN  EIK   
Sbjct: 247 RFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNSKEIKQHG 306

Query: 313 NSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKK 372
           N+ + G+++  VIEEC+LF+ AGQETT+ LL+WTM+LLS + DWQ+RA+ EV   FGN K
Sbjct: 307 NT-NFGLTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNK 366

Query: 373 PDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRD 432
           PD+DGLN LKVV MI  EVLRLYPP+  L RT  +E KLG ++LP G++L LPIIL+  D
Sbjct: 367 PDFDGLNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYD 426

Query: 433 PELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQH 492
            E+WG+DA EFNPERFSEGV KATK    + PF WGPRICIGQNFAM+EAKMA++MILQ 
Sbjct: 427 QEIWGDDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQR 486

Query: 493 FSFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           FSF LSPSY H P A +T QPQ+GAH+ILH L
Sbjct: 487 FSFVLSPSYAHAPHAIITLQPQYGAHLILHSL 517

BLAST of Cucsa.058110 vs. Swiss-Prot
Match: C7A15_ARATH (Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.4e-167
Identity = 278/511 (54.40%), Postives = 377/511 (73.78%), Query Frame = 1

Query: 14  IITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEAL 73
           +  S+VL ++ +  W+   W+WF P+ LE  LRRQG  G  Y  L GD+K++  M  EA 
Sbjct: 8   VTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEAR 67

Query: 74  SKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRP 133
           SKP+K ++ I+PRV+P      + YG+    W GP+P + IMDPEQ+K VF+   DFQ+P
Sbjct: 68  SKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKP 127

Query: 134 TTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVS 193
            T P   ++  GL + +G+KW KHR+IINPAFH+EK+K MVPAF+ SC E+V +W+ +VS
Sbjct: 128 HTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVS 187

Query: 194 REGS-CELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGW 253
            +GS CE+DV P L +M ADVISRTAFGSSY+ G++IF+LQ EL  L+IQA  + +IPG+
Sbjct: 188 DKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGY 247

Query: 254 RFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEIKHGRN 313
            +LPTKSNR++K   REI  ++ GI+N++ ++ +AGE    DLL IL+ESN+      R 
Sbjct: 248 SYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNL------RQ 307

Query: 314 SKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKP 373
           ++  GMS + ++EEC+LFY AGQETT+ LL+WTMVLLS + DWQ RAR EV ++FG+K+P
Sbjct: 308 TEGNGMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEP 367

Query: 374 DYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDP 433
           D +GLN+LKV+TMI  EVLRLYPPV+ L R   KE KLG++TLPGG+ +SLPI+L+Q D 
Sbjct: 368 DAEGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDI 427

Query: 434 ELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHF 493
           ELWG DA EFNP+RF +G+ KATK+  +F PF WGPRICIGQNFA++EAKMA+++IL+ F
Sbjct: 428 ELWGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRF 487

Query: 494 SFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           SFE+SPSY H P+  +T  PQ GA +I+HKL
Sbjct: 488 SFEISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of Cucsa.058110 vs. Swiss-Prot
Match: C7A14_ARATH (Cytochrome P450 72A14 OS=Arabidopsis thaliana GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.2e-165
Identity = 279/508 (54.92%), Postives = 376/508 (74.02%), Query Frame = 1

Query: 17  SLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEALSKP 76
           SL ++++ +  W+   W+WF P+ LE+ LRRQG +G SY  L GD K+  +M  EA SKP
Sbjct: 11  SLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKP 70

Query: 77  MKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRPTTD 136
           +K ++ I PRV+P     ++ +G+ ++ W GP+P + IMDPEQ+K VF+   DFQ+  T 
Sbjct: 71  IKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTF 130

Query: 137 PFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVSREG 196
           P  ++L  GLVS +G+KW +HR+IINPAFHLEK+K MV  F+ SC+E+V +W+ +VS +G
Sbjct: 131 PLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKG 190

Query: 197 S-CELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGWRFL 256
           S CE+DV P L +M ADVISRTAFGSSY  G +IF+LQ EL  LV+QA  + +IPG+ +L
Sbjct: 191 SSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYL 250

Query: 257 PTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEIKHGRNSKD 316
           PTK NR++K   REI  ++ GIIN++E++ ++GE    DLL IL+ESN+ + +       
Sbjct: 251 PTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNLGQTEGN----- 310

Query: 317 VGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKPDYD 376
            GMS + ++EEC+LFY+AGQETT+ LL+WTMVLLS + DWQ RAR EV ++FG+K+PD +
Sbjct: 311 -GMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTE 370

Query: 377 GLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDPELW 436
           GLN+LKV+TMI  EVLRLYPPV  L R   KE KLG++TLPGG+ +SLP++L+ RD ELW
Sbjct: 371 GLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELW 430

Query: 437 GEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHFSFE 496
           G DA EF PERF +G+ KATKN  +F PF WGPRICIGQNF ++EAKMA+S+ILQ FSFE
Sbjct: 431 GNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFE 490

Query: 497 LSPSYTHTPFAALTTQPQHGAHIILHKL 524
           LSPSY H P+  +T  PQ GAH++LHKL
Sbjct: 491 LSPSYVHAPYTIITLYPQFGAHLMLHKL 512

BLAST of Cucsa.058110 vs. Swiss-Prot
Match: C7A13_ARATH (Cytochrome P450 72A13 OS=Arabidopsis thaliana GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.4e-164
Identity = 276/511 (54.01%), Postives = 373/511 (72.99%), Query Frame = 1

Query: 14  IITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEAL 73
           +  S+ ++++ +  W+    +W  P+ LE  LRRQG  G  Y  L GD+K + +M  EA 
Sbjct: 8   VTVSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEAR 67

Query: 74  SKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRP 133
           SKP+  ++ I PR++P     ++ +G+    W GP+P + IMDPEQ+K VF+   DFQ+ 
Sbjct: 68  SKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKA 127

Query: 134 TTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVS 193
            T P  RL+  GLVS +G+KW KHR+IINPAFHLEK+K MVPAF+ SC+E+V +W+ +V+
Sbjct: 128 HTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVT 187

Query: 194 -REGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGW 253
            ++ SCE+D+ P+L +M ADVISRTAFGSSY+ G++IF+LQ EL  L+IQA  +  IPG+
Sbjct: 188 DKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGY 247

Query: 254 RFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEIKHGRN 313
           R+ PTK NR++K   REI  ++ GI+N++ ++ +AGE    DLL IL+ESN+ + K    
Sbjct: 248 RYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSDDLLGILLESNLGQTKGN-- 307

Query: 314 SKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKP 373
               GMS + ++EEC+LFY AGQETT  LL+WTMVLLS + DWQ RAR EV ++FG+K+P
Sbjct: 308 ----GMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEP 367

Query: 374 DYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDP 433
           D +GLN+LKV+TMI  EVLRLYPPV  L R   KE +LG++TLPGG+ +SLPI+LIQRD 
Sbjct: 368 DAEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDR 427

Query: 434 ELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHF 493
           ELWG DA EF P+RF +G+ KATKN  +F PF WGPRICIGQNFA++EAKMA+++IL+ F
Sbjct: 428 ELWGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKF 487

Query: 494 SFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           SFELSPSY H P+  LTT PQ GA +ILHKL
Sbjct: 488 SFELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of Cucsa.058110 vs. Swiss-Prot
Match: C72A1_CATRO (Secologanin synthase OS=Catharanthus roseus GN=CYP72A1 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 7.9e-163
Identity = 264/511 (51.66%), Postives = 368/511 (72.02%), Query Frame = 1

Query: 15  ITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEALS 74
           I + +  L++   W++ DW WF P+R+E+ LR+QGF GN Y+ L GD+KES  M +EALS
Sbjct: 12  IAATIFALVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQEALS 71

Query: 75  KPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQR-- 134
           KPM+F+N I PR++P + HTI  YG+NS  W+G +PR+H+M+PE +K V +  + +Q+  
Sbjct: 72  KPMEFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNF 131

Query: 135 PTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMV 194
              +P ++ L+ G+ S EG KW KHR+II+PAF LEKLK+M+PAF    ++M++KWE + 
Sbjct: 132 DVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKWEKIA 191

Query: 195 SREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGW 254
            ++GS E+D+ P    + +DVIS+ AFGS+YE G KIF+L  EL +L I    + YIPGW
Sbjct: 192 EKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDVYIPGW 251

Query: 255 RFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEIKHGRN 314
            +LPTK N+++KE N+EIT ++  IIN++ K++KAGE  + DLL +L+ESN+ EI+   N
Sbjct: 252 SYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGEPGEDDLLGVLLESNIQEIQKQGN 311

Query: 315 SKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKP 374
            KD GMSI  VIEEC+LFY AGQETT  LL WT +LLS + +WQERAR EV + FG  KP
Sbjct: 312 KKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKP 371

Query: 375 DYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDP 434
           +++ LN LK V+MI  EVLRLYPPV  L +   K+TKLG+ T+P G  + LP +++ R+ 
Sbjct: 372 EFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVMLPTVMLHREK 431

Query: 435 ELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHF 494
            +WGEDA EFNP RF +GV  ATKN   ++PF WGPR+C+GQNFA+++AK+ L+MILQ F
Sbjct: 432 SIWGEDAMEFNPMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLGLAMILQRF 491

Query: 495 SFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
            F+++PSY H PF  LT QPQ G+H+I  KL
Sbjct: 492 KFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522

BLAST of Cucsa.058110 vs. TrEMBL
Match: V7BTM1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G063300g PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 4.9e-188
Identity = 310/509 (60.90%), Postives = 400/509 (78.59%), Query Frame = 1

Query: 17  SLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEALSKP 76
           +L+++LLL   WK+ +W+WF P+RLE+LLR QGF GN YK+L GD KE   MRKEALSKP
Sbjct: 10  TLIVILLLTWAWKILNWLWFNPKRLEKLLREQGFQGNPYKLLVGDSKEFLKMRKEALSKP 69

Query: 77  MKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRPTTD 136
           M  S+ I PRV   + H+++ +GKNS +WLGP PRV I+DPEQ+K +F+   DF +P  +
Sbjct: 70  MDLSDDIVPRVSSYINHSVKSHGKNSFIWLGPTPRVTILDPEQIKDIFNKIYDFPKPDIN 129

Query: 137 PFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVSREG 196
           P  +LL  GL   EGEKW KHR++INPAF+LEKLKAM+P F+ SCN++V+KWE M+S +G
Sbjct: 130 PLFKLLATGLAGYEGEKWSKHRRLINPAFNLEKLKAMLPLFFESCNDLVNKWERMLSSDG 189

Query: 197 SCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGWRFLP 256
           SCE+D  P+LQNM +DVI+R+AFGSS+E G++IF+LQ E T L +Q  L+  IPGWRFLP
Sbjct: 190 SCEIDAWPFLQNMTSDVIARSAFGSSFEEGRRIFQLQREQTKLALQVILKVQIPGWRFLP 249

Query: 257 TKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEI-KHGR-NSK 316
           TKS+R++KE +R+I   +  +I ++EK++K GE I  DLL IL+ESN  EI +H + ++K
Sbjct: 250 TKSHRRMKEIDRDIKASLKDMIYKREKALKKGEAINDDLLGILLESNHKEIQEHAKGDNK 309

Query: 317 DVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKPDY 376
           +VGMS++ VIEEC+LFY AGQETT+ LL+WTMVLLS Y DWQERAR EV ++FGN+KP +
Sbjct: 310 NVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQERAREEVLQVFGNQKPSF 369

Query: 377 DGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDPEL 436
           DGLNRLK+VTMI NEVLRLYPP   + R  +++ KLGN+TLP G+ + LP +L+  D E 
Sbjct: 370 DGLNRLKIVTMILNEVLRLYPPALGMNRLAKRDLKLGNITLPAGVQIFLPTVLVHHDCEF 429

Query: 437 WGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHFSF 496
           WGEDA +FNPERFSEGV KATK   +F PFGWGPRICIGQNF+++EAKMALSMILQHFSF
Sbjct: 430 WGEDAKQFNPERFSEGVLKATKGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQHFSF 489

Query: 497 ELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           ELSP+Y H P   +T QPQ+GAHIILHK+
Sbjct: 490 ELSPAYAHAPAMLITLQPQYGAHIILHKV 518

BLAST of Cucsa.058110 vs. TrEMBL
Match: V7BXP5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G063200g PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 1.6e-186
Identity = 307/509 (60.31%), Postives = 399/509 (78.39%), Query Frame = 1

Query: 17  SLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEALSKP 76
           +L+++LLL   WK+ +W+WF P+RLE+LLR QGF GN YK+L GD KE   MRKEALSKP
Sbjct: 10  TLIVILLLIWAWKILNWLWFNPKRLEKLLREQGFQGNPYKLLVGDSKEFLKMRKEALSKP 69

Query: 77  MKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRPTTD 136
           M  S+ I PRV   + H+++ +GKNS +WLGP PRV I+DPEQ+K +F+   DF +P  +
Sbjct: 70  MDLSDDIVPRVSSYINHSVKSHGKNSFIWLGPTPRVTILDPEQIKDIFNKIYDFPKPDIN 129

Query: 137 PFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVSREG 196
           P ++LL  GL   EGEKW KHR++INPAF+LEKLKAM+P F+ SCN++V+KWE M+S +G
Sbjct: 130 PLLKLLATGLADYEGEKWSKHRRLINPAFNLEKLKAMLPLFFESCNDLVNKWERMLSSDG 189

Query: 197 SCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGWRFLP 256
           SCE+D  P+LQNM +DVI+R+AFGSS+E G++IF+LQ E   L +Q  L+  IPGWRFLP
Sbjct: 190 SCEIDAWPFLQNMTSDVIARSAFGSSFEEGRRIFQLQREQAKLALQVILKIQIPGWRFLP 249

Query: 257 TKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEI-KHGR-NSK 316
           TKS+R++KE +R+I   +  +I ++EK++K GE I  DLL IL+ESN  EI +H + ++K
Sbjct: 250 TKSHRRMKEIDRDIKASLKDMIYKREKALKKGEAINDDLLGILLESNHKEIQEHAKGDNK 309

Query: 317 DVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKPDY 376
           +VGMS++ VIEEC+LFY AGQETT+ LL+WTMVLLS Y DWQERAR EV ++FGN+KP +
Sbjct: 310 NVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQERAREEVLQVFGNQKPSF 369

Query: 377 DGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDPEL 436
           DGLNRLK+VTMI NEVLRLYPP   + R  +++ KLGN+ LP G+ + LP +L+  + E 
Sbjct: 370 DGLNRLKIVTMILNEVLRLYPPALGMNRLAKRDLKLGNIILPAGVQIFLPTVLVHHNCEF 429

Query: 437 WGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHFSF 496
           WGEDA +FNPERFSEGV KATK   +F PFGWGPRICIGQNF+++EAKMALSMILQHFSF
Sbjct: 430 WGEDAKQFNPERFSEGVLKATKGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQHFSF 489

Query: 497 ELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           ELSP+Y H P   +T QPQ+GAHIILHK+
Sbjct: 490 ELSPAYAHAPAMLITLQPQYGAHIILHKV 518

BLAST of Cucsa.058110 vs. TrEMBL
Match: A0A0B2SBC8_GLYSO (Secologanin synthase OS=Glycine soja GN=glysoja_039974 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 1.0e-185
Identity = 310/511 (60.67%), Postives = 397/511 (77.69%), Query Frame = 1

Query: 15  ITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEALS 74
           I  L+L+L L   WK+ +W+W  P+RLE+LLR QGF GN Y +  GD KE   MRKEA+S
Sbjct: 8   ILLLILILALICSWKVLNWLWLRPKRLEKLLREQGFRGNPYTLFFGDSKEFLKMRKEAVS 67

Query: 75  KPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRPT 134
           KPM  S+ I PRV   V H++ ++GKNS +W GP+PRV I+DPEQ+K VF+   DF +P 
Sbjct: 68  KPMTLSDDIVPRVSAYVQHSVNKHGKNSFIWFGPMPRVTILDPEQIKDVFNKNYDFPKPN 127

Query: 135 TDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVSR 194
            +P ++LL  GL   EGEKW KHR+IINPAF+LEKLK M+P F  SCN++VSKWE M+S 
Sbjct: 128 LNPLVKLLATGLAGYEGEKWSKHRRIINPAFNLEKLKIMLPLFLQSCNDLVSKWEGMLSS 187

Query: 195 EGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGWRF 254
           EGSCE+D  P+LQN+ +DVI+R+AFGSSYE G++IF+LQ E T L+++  L+  IPGWRF
Sbjct: 188 EGSCEMDAWPFLQNLTSDVIARSAFGSSYEEGRRIFQLQREQTELLMKVILKIQIPGWRF 247

Query: 255 LPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEI-KHG-RN 314
           LPTK++R++KE +REI   +  +IN++EK++K+GE  + DLL IL+ESN  EI +HG RN
Sbjct: 248 LPTKTHRRMKEIDREIKASLKNMINKREKALKSGEATKNDLLGILLESNHKEIQEHGNRN 307

Query: 315 SKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKP 374
           SK+VGMS++ VIEEC+LFY AGQETT+ LL+WTMVLLS Y DWQ RAR E F++FG +KP
Sbjct: 308 SKNVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEAFQVFGYQKP 367

Query: 375 DYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDP 434
           D+DGL+RLK+VTMI  EVLRLYPP+  + R   K+ KLGN+TLP G+ + LP +LI  D 
Sbjct: 368 DFDGLSRLKIVTMILYEVLRLYPPLIGMNRLVEKDVKLGNLTLPAGVQVFLPTVLIHHDS 427

Query: 435 ELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHF 494
           ELWGEDA +FNPERFSEGV KAT    +F PFGWGPRICIGQNF+++EAKMALSMILQ+F
Sbjct: 428 ELWGEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQNF 487

Query: 495 SFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
            FELSP+Y H P  ++TTQPQ+GAHIIL K+
Sbjct: 488 LFELSPAYAHAPTMSITTQPQYGAHIILRKV 518

BLAST of Cucsa.058110 vs. TrEMBL
Match: I1MIY4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G244200 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 3.9e-185
Identity = 311/511 (60.86%), Postives = 397/511 (77.69%), Query Frame = 1

Query: 15  ITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEALS 74
           I +L+L+L L   WK+  W+W  P+RLE+LLR QG  GN Y +  GD KE   MRKEALS
Sbjct: 8   ILTLILILALICAWKVLIWLWLRPKRLEKLLREQGLQGNPYTLFVGDSKEFLKMRKEALS 67

Query: 75  KPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRPT 134
           KPM  S+ I PRV     H++ ++GKNS +WLGP+PRV I+DPEQ+K VF+   DF +P 
Sbjct: 68  KPMNLSDDIIPRVSSYEQHSVNKHGKNSFIWLGPIPRVTILDPEQIKDVFNKIYDFPKPN 127

Query: 135 TDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVSR 194
            +P ++LL  GL   EGEKW KHR+IINPAF+LEKLK M+P F+ SCN++VSKWE M+S 
Sbjct: 128 MNPLVKLLATGLAGYEGEKWSKHRRIINPAFNLEKLKIMLPLFFKSCNDLVSKWEGMLSS 187

Query: 195 EGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGWRF 254
           EGSCE+D  P+LQN+A+DVI+R+AFGSSYE G++IF+LQ E   L+I+  L+  IPGWRF
Sbjct: 188 EGSCEMDAWPFLQNLASDVIARSAFGSSYEEGRRIFQLQREQAELLIKVLLKIQIPGWRF 247

Query: 255 LPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEI-KHG-RN 314
           LPT ++R++KE +R+I   +  +IN++EK++KAGE  + DLL IL+ESN  EI +HG RN
Sbjct: 248 LPTNTHRRMKEIDRDIKASLKDMINKREKALKAGEATKNDLLGILLESNHKEIQEHGNRN 307

Query: 315 SKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKP 374
           +K+VGMS++ VIEEC+LFY AGQETT+ LL+WTMVLLS Y DWQ RAR EVF++FG +KP
Sbjct: 308 NKNVGMSLEEVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVFQVFGYQKP 367

Query: 375 DYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDP 434
           D+DGL+RLK+VTMI  EVLRLYPPV+ + R+  K+ KLG +TLP G+ + LP ILI  D 
Sbjct: 368 DFDGLSRLKIVTMILYEVLRLYPPVAGMTRSIEKDVKLGTLTLPAGVHVLLPTILIHHDR 427

Query: 435 ELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHF 494
           + WGEDA +FNPERFSEGV KAT    +F PFGWGPRICIGQNF+++EAKMALSMILQHF
Sbjct: 428 KFWGEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQHF 487

Query: 495 SFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           SFELSP+Y H P A +T QPQ+GAHIIL K+
Sbjct: 488 SFELSPAYAHAPTALITIQPQYGAHIILRKV 518

BLAST of Cucsa.058110 vs. TrEMBL
Match: I1MIY5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G244300 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 6.6e-185
Identity = 309/511 (60.47%), Postives = 396/511 (77.50%), Query Frame = 1

Query: 15  ITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEALS 74
           I  L+L+L L   WK+ +W+W  P+RLE+LLR QGF GN Y +  GD KE   MRKEA+S
Sbjct: 8   ILLLILILALICSWKVLNWLWLRPKRLEKLLREQGFRGNPYTLFFGDSKEFLKMRKEAVS 67

Query: 75  KPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRPT 134
           KPM  S+ I PRV   V H++ ++GKNS +W GP+PRV I+DPEQ+K VF+   DF +P 
Sbjct: 68  KPMTLSDDIVPRVSAYVQHSVNKHGKNSFIWFGPMPRVTILDPEQIKDVFNKNYDFPKPN 127

Query: 135 TDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVSR 194
            +P ++LL  GL   EGEKW KHR+IINPAF+LEKLK M+P F  SCN++VSKWE M+S 
Sbjct: 128 LNPLVKLLATGLAGYEGEKWSKHRRIINPAFNLEKLKIMLPLFLQSCNDLVSKWEGMLSS 187

Query: 195 EGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGWRF 254
           EGSCE+D  P+LQN+ +DVI+R+AFGSSYE G++IF+LQ E T  +++  L+  IPGWRF
Sbjct: 188 EGSCEMDAWPFLQNLTSDVIARSAFGSSYEEGRRIFQLQREQTEHLMKVILKIQIPGWRF 247

Query: 255 LPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEI-KHG-RN 314
           LPTK++R++KE +REI   +  +IN++EK++K+GE  + DLL IL+ESN  EI +HG RN
Sbjct: 248 LPTKTHRRMKEIDREIKASLKNMINKREKALKSGEATKNDLLGILLESNHKEIQEHGNRN 307

Query: 315 SKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKP 374
           SK+VGMS++ VIEEC+LFY AGQETT+ LL+WTMVLLS Y DWQ RAR E F++FG +KP
Sbjct: 308 SKNVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEAFQVFGYQKP 367

Query: 375 DYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDP 434
           D+DGL+RLK+VTMI  EVLRLYPP+  + R   K+ KLGN+TLP G+ + LP +LI  D 
Sbjct: 368 DFDGLSRLKIVTMILYEVLRLYPPLIGMNRLVEKDVKLGNLTLPAGVQVFLPTVLIHHDS 427

Query: 435 ELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHF 494
           ELWGEDA +FNPERFSEGV KAT    +F PFGWGPRICIGQNF+++EAKMALSMILQ+F
Sbjct: 428 ELWGEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQNF 487

Query: 495 SFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
            FELSP+Y H P  ++TTQPQ+GAHIIL K+
Sbjct: 488 LFELSPAYAHAPTMSITTQPQYGAHIILRKV 518

BLAST of Cucsa.058110 vs. TAIR10
Match: AT3G14610.1 (AT3G14610.1 cytochrome P450, family 72, subfamily A, polypeptide 7)

HSP 1 Score: 593.2 bits (1528), Expect = 1.6e-169
Identity = 284/518 (54.83%), Postives = 377/518 (72.78%), Query Frame = 1

Query: 8   ISTPIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAA 67
           +S  +V    +++ +++   W++  W+W  P+ LE  L+RQG TG  Y  L GD+K +  
Sbjct: 1   MSFSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVD 60

Query: 68  MRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFF 127
           M  EA SKP+  ++ I PR++P     +  +GK   +W+GPLP + I +PEQ+K VF+  
Sbjct: 61  MMMEARSKPINVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKV 120

Query: 128 NDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSK 187
           NDF++ +T P IRLL  GL S +G+KW  HR+IINPAFHLEK+K M+PAFYH C+E+V +
Sbjct: 121 NDFEKASTFPLIRLLAGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQ 180

Query: 188 WESMVS-REGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLE 247
           WE + + +E   E+DV P+L NM ADVIS TAFGSSY+ G++IF+LQ EL  L+ QA  +
Sbjct: 181 WEKLFTDKESPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKK 240

Query: 248 TYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNE 307
           +YIPG RF PTKSNR++K  +RE+  ++ GI++++EK+ +AGE    DLL IL+ESN  E
Sbjct: 241 SYIPGSRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEPANDDLLGILLESNSEE 300

Query: 308 IKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEI 367
                 S+  GMS++ V++EC+LFY AGQETT+ LL+WTMVLLS + DWQ RAR EV ++
Sbjct: 301 ------SQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQV 360

Query: 368 FG-NKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPI 427
            G N KPD + LN LKV+TMIFNEVLRLYPPV+ L R   KE KLG +TLP GI + LP 
Sbjct: 361 LGENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPT 420

Query: 428 ILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMAL 487
           IL+QRD ELWG+DA +F PERF +G+ KATKN  +F PFGWGPRICIGQNFAM+EAKMA+
Sbjct: 421 ILVQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAM 480

Query: 488 SMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           ++ILQ FSFELSPSY H P   +TT+PQ GAH+ILHKL
Sbjct: 481 ALILQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of Cucsa.058110 vs. TAIR10
Match: AT3G14690.1 (AT3G14690.1 cytochrome P450, family 72, subfamily A, polypeptide 15)

HSP 1 Score: 590.9 bits (1522), Expect = 7.8e-169
Identity = 278/511 (54.40%), Postives = 377/511 (73.78%), Query Frame = 1

Query: 14  IITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEAL 73
           +  S+VL ++ +  W+   W+WF P+ LE  LRRQG  G  Y  L GD+K++  M  EA 
Sbjct: 8   VTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEAR 67

Query: 74  SKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRP 133
           SKP+K ++ I+PRV+P      + YG+    W GP+P + IMDPEQ+K VF+   DFQ+P
Sbjct: 68  SKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKP 127

Query: 134 TTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVS 193
            T P   ++  GL + +G+KW KHR+IINPAFH+EK+K MVPAF+ SC E+V +W+ +VS
Sbjct: 128 HTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVS 187

Query: 194 REGS-CELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGW 253
            +GS CE+DV P L +M ADVISRTAFGSSY+ G++IF+LQ EL  L+IQA  + +IPG+
Sbjct: 188 DKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGY 247

Query: 254 RFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEIKHGRN 313
            +LPTKSNR++K   REI  ++ GI+N++ ++ +AGE    DLL IL+ESN+      R 
Sbjct: 248 SYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNL------RQ 307

Query: 314 SKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKP 373
           ++  GMS + ++EEC+LFY AGQETT+ LL+WTMVLLS + DWQ RAR EV ++FG+K+P
Sbjct: 308 TEGNGMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEP 367

Query: 374 DYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDP 433
           D +GLN+LKV+TMI  EVLRLYPPV+ L R   KE KLG++TLPGG+ +SLPI+L+Q D 
Sbjct: 368 DAEGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDI 427

Query: 434 ELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHF 493
           ELWG DA EFNP+RF +G+ KATK+  +F PF WGPRICIGQNFA++EAKMA+++IL+ F
Sbjct: 428 ELWGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRF 487

Query: 494 SFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           SFE+SPSY H P+  +T  PQ GA +I+HKL
Sbjct: 488 SFEISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of Cucsa.058110 vs. TAIR10
Match: AT3G14630.1 (AT3G14630.1 cytochrome P450, family 72, subfamily A, polypeptide 9)

HSP 1 Score: 589.7 bits (1519), Expect = 1.7e-168
Identity = 281/512 (54.88%), Postives = 373/512 (72.85%), Query Frame = 1

Query: 13  VIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEA 72
           ++I SL L+++L+  W++ +W+W  P+ LE  LRRQG  G  Y  L GD++ S +M KEA
Sbjct: 3   IVIASLALVVVLWCIWRILEWVWLKPKMLESYLRRQGLVGTRYTPLVGDVRRSFSMLKEA 62

Query: 73  LSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQR 132
            SKPMK ++ +   V+P  +H +  YGK    W GP+P + IM+P+ +K V++ F DF++
Sbjct: 63  RSKPMKPTDDLISLVMPYSFHMLNTYGKTFFTWSGPIPAITIMNPQLIKEVYNKFYDFEK 122

Query: 133 PTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMV 192
             T P   LL  GL + +G+KWVKHRKIINPAFH EK+K MVP FY SC E++ +WE +V
Sbjct: 123 THTFPLTSLLTDGLANADGDKWVKHRKIINPAFHFEKIKNMVPTFYKSCIEVMCEWEKLV 182

Query: 193 SREGS-CELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPG 252
           S +GS CELDV P++ NM  DVISRTAFGSSY+ G++IF LQ EL +L+I A  + YIP 
Sbjct: 183 SDKGSSCELDVWPWIVNMTGDVISRTAFGSSYKEGQRIFILQAELAHLIILALGKNYIPA 242

Query: 253 WRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEIKHGR 312
           +R  PTK+NR++K   +EI  ++ GII+ +EK+  AGE    DLL IL++SN  +     
Sbjct: 243 YRHFPTKNNRRMKTIVKEIQVILRGIISHREKARDAGEAPSDDLLGILLKSNSEQ----- 302

Query: 313 NSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKK 372
            SK  G++++ ++EEC+LFY AGQETT+ LL WTMVLLS + DWQ RAR EV ++FG+ K
Sbjct: 303 -SKGNGLNMEEIMEECKLFYFAGQETTSVLLAWTMVLLSQHQDWQARAREEVMQVFGHNK 362

Query: 373 PDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRD 432
           PD  G+N+LKV+TMI  EVLRLYPPV  + R T KE KLG+MTLPGGI + +P++LI RD
Sbjct: 363 PDLQGINQLKVMTMIIYEVLRLYPPVIQMNRATHKEIKLGDMTLPGGIQVHMPVLLIHRD 422

Query: 433 PELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQH 492
            +LWG+DA EF PERF +G+ KATKN   F+PFGWGPRICIGQNFA++EAKMAL++ILQ 
Sbjct: 423 TKLWGDDAAEFKPERFKDGIAKATKNQVCFLPFGWGPRICIGQNFALLEAKMALALILQR 482

Query: 493 FSFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           FSFELSPSY H+P+   T  PQ GAH+ILHKL
Sbjct: 483 FSFELSPSYVHSPYRVFTIHPQCGAHLILHKL 508

BLAST of Cucsa.058110 vs. TAIR10
Match: AT3G14680.1 (AT3G14680.1 cytochrome P450, family 72, subfamily A, polypeptide 14)

HSP 1 Score: 583.6 bits (1503), Expect = 1.2e-166
Identity = 279/508 (54.92%), Postives = 376/508 (74.02%), Query Frame = 1

Query: 17  SLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEALSKP 76
           SL ++++ +  W+   W+WF P+ LE+ LRRQG +G SY  L GD K+  +M  EA SKP
Sbjct: 11  SLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKP 70

Query: 77  MKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRPTTD 136
           +K ++ I PRV+P     ++ +G+ ++ W GP+P + IMDPEQ+K VF+   DFQ+  T 
Sbjct: 71  IKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTF 130

Query: 137 PFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVSREG 196
           P  ++L  GLVS +G+KW +HR+IINPAFHLEK+K MV  F+ SC+E+V +W+ +VS +G
Sbjct: 131 PLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKG 190

Query: 197 S-CELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGWRFL 256
           S CE+DV P L +M ADVISRTAFGSSY  G +IF+LQ EL  LV+QA  + +IPG+ +L
Sbjct: 191 SSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYL 250

Query: 257 PTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEIKHGRNSKD 316
           PTK NR++K   REI  ++ GIIN++E++ ++GE    DLL IL+ESN+ + +       
Sbjct: 251 PTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNLGQTEGN----- 310

Query: 317 VGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKPDYD 376
            GMS + ++EEC+LFY+AGQETT+ LL+WTMVLLS + DWQ RAR EV ++FG+K+PD +
Sbjct: 311 -GMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTE 370

Query: 377 GLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDPELW 436
           GLN+LKV+TMI  EVLRLYPPV  L R   KE KLG++TLPGG+ +SLP++L+ RD ELW
Sbjct: 371 GLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELW 430

Query: 437 GEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHFSFE 496
           G DA EF PERF +G+ KATKN  +F PF WGPRICIGQNF ++EAKMA+S+ILQ FSFE
Sbjct: 431 GNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFE 490

Query: 497 LSPSYTHTPFAALTTQPQHGAHIILHKL 524
           LSPSY H P+  +T  PQ GAH++LHKL
Sbjct: 491 LSPSYVHAPYTIITLYPQFGAHLMLHKL 512

BLAST of Cucsa.058110 vs. TAIR10
Match: AT3G14660.1 (AT3G14660.1 cytochrome P450, family 72, subfamily A, polypeptide 13)

HSP 1 Score: 580.1 bits (1494), Expect = 1.4e-165
Identity = 276/511 (54.01%), Postives = 373/511 (72.99%), Query Frame = 1

Query: 14  IITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHGDMKESAAMRKEAL 73
           +  S+ ++++ +  W+    +W  P+ LE  LRRQG  G  Y  L GD+K + +M  EA 
Sbjct: 8   VTVSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEAR 67

Query: 74  SKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQLKTVFSFFNDFQRP 133
           SKP+  ++ I PR++P     ++ +G+    W GP+P + IMDPEQ+K VF+   DFQ+ 
Sbjct: 68  SKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKA 127

Query: 134 TTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHSCNEMVSKWESMVS 193
            T P  RL+  GLVS +G+KW KHR+IINPAFHLEK+K MVPAF+ SC+E+V +W+ +V+
Sbjct: 128 HTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVT 187

Query: 194 -REGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLVIQASLETYIPGW 253
            ++ SCE+D+ P+L +M ADVISRTAFGSSY+ G++IF+LQ EL  L+IQA  +  IPG+
Sbjct: 188 DKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGY 247

Query: 254 RFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILMESNMNEIKHGRN 313
           R+ PTK NR++K   REI  ++ GI+N++ ++ +AGE    DLL IL+ESN+ + K    
Sbjct: 248 RYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSDDLLGILLESNLGQTKGN-- 307

Query: 314 SKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERARAEVFEIFGNKKP 373
               GMS + ++EEC+LFY AGQETT  LL+WTMVLLS + DWQ RAR EV ++FG+K+P
Sbjct: 308 ----GMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEP 367

Query: 374 DYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIMLSLPIILIQRDP 433
           D +GLN+LKV+TMI  EVLRLYPPV  L R   KE +LG++TLPGG+ +SLPI+LIQRD 
Sbjct: 368 DAEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDR 427

Query: 434 ELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIEAKMALSMILQHF 493
           ELWG DA EF P+RF +G+ KATKN  +F PF WGPRICIGQNFA++EAKMA+++IL+ F
Sbjct: 428 ELWGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKF 487

Query: 494 SFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           SFELSPSY H P+  LTT PQ GA +ILHKL
Sbjct: 488 SFELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of Cucsa.058110 vs. NCBI nr
Match: gi|778695577|ref|XP_004146006.2| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 1061.6 bits (2744), Expect = 4.4e-307
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 1

Query: 1   MWYVPHMISTPIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHG 60
           MWYVPHMISTPIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHG
Sbjct: 1   MWYVPHMISTPIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHG 60

Query: 61  DMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQL 120
           DMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQL
Sbjct: 61  DMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQL 120

Query: 121 KTVFSFFNDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHS 180
           KTVFSFFNDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHS
Sbjct: 121 KTVFSFFNDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHS 180

Query: 181 CNEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLV 240
           CNEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLV
Sbjct: 181 CNEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLV 240

Query: 241 IQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILM 300
           IQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILM
Sbjct: 241 IQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILM 300

Query: 301 ESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERAR 360
           ESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERAR
Sbjct: 301 ESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERAR 360

Query: 361 AEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIM 420
           AEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIM
Sbjct: 361 AEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIM 420

Query: 421 LSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIE 480
           LSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIE
Sbjct: 421 LSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIE 480

Query: 481 AKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           AKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL
Sbjct: 481 AKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 523

BLAST of Cucsa.058110 vs. NCBI nr
Match: gi|778695562|ref|XP_011654016.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 899.0 bits (2322), Expect = 3.8e-258
Identity = 430/523 (82.22%), Postives = 478/523 (91.40%), Query Frame = 1

Query: 1   MWYVPHMISTPIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHG 60
           MWY P  I        S+VL+LLL LGWKLADWIWF P++LE+LLR+QGF GNSY+ILHG
Sbjct: 1   MWYAPDTI-------ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHG 60

Query: 61  DMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQL 120
           D+KE AAMR+EA+SKPM FSNHIAPRVIPS+YHTIQRYGKNS MWLGP+PRVHIMDPEQL
Sbjct: 61  DLKERAAMREEAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQL 120

Query: 121 KTVFSFFNDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHS 180
           KTVFSF+NDFQ+PT +PF +LL+ G+V+L+GEKWVKHRKIINPAFHLEKLK MVPAFYHS
Sbjct: 121 KTVFSFYNDFQKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHS 180

Query: 181 CNEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLV 240
           CNEMVSKWESMVS+EGSCELDVMPYL+NMA+DVISRTAFGSSYE+GKKIFKLQTELTN V
Sbjct: 181 CNEMVSKWESMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFV 240

Query: 241 IQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILM 300
           IQ +L  YIPGWRFLPTK NRK+KE ++EITTL+LGI++E+EKSMKAGE IQTDLLSILM
Sbjct: 241 IQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILM 300

Query: 301 ESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERAR 360
           ESNMNEIK   N KD+GMSI+ VIEEC+LFYIAGQETTATLL+WTM+LLSSYS+WQERAR
Sbjct: 301 ESNMNEIKQHGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERAR 360

Query: 361 AEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIM 420
           AEVFEIFGNK+P+YDGLNRLKVVTMIFNEVLRLYPP S+  R  RKET+LGN+TLPGG+M
Sbjct: 361 AEVFEIFGNKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVM 420

Query: 421 LSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIE 480
           L LPI+LIQRDPELWGEDAHEFNPERFS GV KATKNP AF+PFGWGPRICIGQ FAMIE
Sbjct: 421 LGLPIVLIQRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIE 480

Query: 481 AKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           AKMALSMILQ FSFELSPSYTH+P A+LTTQPQHGAHIILHKL
Sbjct: 481 AKMALSMILQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 516

BLAST of Cucsa.058110 vs. NCBI nr
Match: gi|659103032|ref|XP_008452438.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 890.6 bits (2300), Expect = 1.3e-255
Identity = 433/524 (82.63%), Postives = 476/524 (90.84%), Query Frame = 1

Query: 1   MWYVPHMISTPIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHG 60
           M YVP  I    VII+ ++LLLLL LGWKL DWIWF P++LE++LRRQGF GNSY+ILHG
Sbjct: 1   MSYVPIHIG---VIISYVLLLLLLLLGWKLVDWIWFRPKKLEKILRRQGFAGNSYRILHG 60

Query: 61  DMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQL 120
           D+KESAAMR+EA+SKPM FSNHIAPRVIPSVYHTIQ YGK SMMWLGP+PRVHIMDPEQL
Sbjct: 61  DLKESAAMREEAMSKPMNFSNHIAPRVIPSVYHTIQLYGKKSMMWLGPMPRVHIMDPEQL 120

Query: 121 KTVFSFFNDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHS 180
           K VFS  NDFQ+PT +P  RLL+ GL SLEG+KWVKHRKIINPAFH+EKLK MVPAFYHS
Sbjct: 121 KIVFSSINDFQKPTMNPLTRLLVQGLASLEGQKWVKHRKIINPAFHVEKLKDMVPAFYHS 180

Query: 181 C-NEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNL 240
           C NEMVSKWE MVSREGSCELDVMPYLQNMA+DVISRTAFGSSYE+GKKIF+LQTEL NL
Sbjct: 181 CYNEMVSKWEGMVSREGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQTELANL 240

Query: 241 VIQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSIL 300
           VIQA+L  YIPGWRFLPTKSNRK+KE ++EITTL+LGI+NEKEKS+KAGE IQTDLLSIL
Sbjct: 241 VIQANLGIYIPGWRFLPTKSNRKMKEISKEITTLILGIMNEKEKSIKAGEAIQTDLLSIL 300

Query: 301 MESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERA 360
           MESNMNEIK   N+K++GMSI+ VIEEC+LFYIAGQETTATLL+WTMVLLSSYS+WQERA
Sbjct: 301 MESNMNEIKQHGNNKNIGMSIEDVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERA 360

Query: 361 RAEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGI 420
           RAEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPV+M GR  RKET+LGN+TLP G+
Sbjct: 361 RAEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVNMFGRIIRKETRLGNLTLPEGV 420

Query: 421 MLSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMI 480
           ML LP++LI  DPELWGEDAHEFNPERFSEG+ KATKNP AF+PFGWGPRICIGQNFAMI
Sbjct: 421 MLGLPVVLIHLDPELWGEDAHEFNPERFSEGISKATKNPSAFIPFGWGPRICIGQNFAMI 480

Query: 481 EAKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           EAKMALS+ILQ FSFELSPSYTH P A LTTQPQHGAHIILHKL
Sbjct: 481 EAKMALSIILQRFSFELSPSYTHAPIAVLTTQPQHGAHIILHKL 521

BLAST of Cucsa.058110 vs. NCBI nr
Match: gi|659103030|ref|XP_008452437.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 867.5 bits (2240), Expect = 1.2e-248
Identity = 421/523 (80.50%), Postives = 470/523 (89.87%), Query Frame = 1

Query: 1   MWYVPHMISTPIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHG 60
           M +V   IS P V+I S+VLLLLL LGWKL DWIWF P+RLE++LRRQGF GNSY+ILHG
Sbjct: 1   MSFVKDTISVPTVMIISIVLLLLL-LGWKLVDWIWFRPKRLERVLRRQGFAGNSYRILHG 60

Query: 61  DMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQL 120
           D+KESAAMR+EA+SKPMKFSNHIAPRVIPSVYHTIQ YGKNS MW+G +PRVHIMDPEQL
Sbjct: 61  DLKESAAMREEAMSKPMKFSNHIAPRVIPSVYHTIQLYGKNSFMWIGTMPRVHIMDPEQL 120

Query: 121 KTVFSFFNDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHS 180
           KTVFS  NDFQRPT  P ++L  +GL S EG+KWVKHRKII+PAFHL+KLK M+P FYHS
Sbjct: 121 KTVFSLINDFQRPTMSPLLKLFANGLFSYEGQKWVKHRKIISPAFHLKKLKDMIPEFYHS 180

Query: 181 CNEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLV 240
            NEMV KWESM+SREGSCELDVMPYLQNMA+DVISRTAFGSSYE+GKKIF+LQ+EL  LV
Sbjct: 181 SNEMVGKWESMISREGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQSELGALV 240

Query: 241 IQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILM 300
           IQ+SL  YIPGWRFLPTKSNRK+KE +++I+TL+LG++NEKEKSMKAGE IQTDLLSIL+
Sbjct: 241 IQSSLGIYIPGWRFLPTKSNRKMKELSKKISTLILGVMNEKEKSMKAGEAIQTDLLSILI 300

Query: 301 ESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERAR 360
           ESN++EIK  RN+K +G+SIQ +I+EC+LFYI GQETTATLLIWTMVLLSSYS WQERAR
Sbjct: 301 ESNLDEIKDHRNNKGMGLSIQDIIDECKLFYITGQETTATLLIWTMVLLSSYSKWQERAR 360

Query: 361 AEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIM 420
           AEVFEIFGNKKPDYDGL+RLKVVTMIFNEVLRLYPPVSM  RT  KETKLG +TLP G+M
Sbjct: 361 AEVFEIFGNKKPDYDGLSRLKVVTMIFNEVLRLYPPVSMYARTVNKETKLGKLTLPAGVM 420

Query: 421 LSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIE 480
           LSLPIILIQ DPELWG+DA+EFNP+RFSEGV KATKNP AF+PFGWGPRICIG NFAMIE
Sbjct: 421 LSLPIILIQTDPELWGQDAYEFNPDRFSEGVSKATKNPFAFVPFGWGPRICIGLNFAMIE 480

Query: 481 AKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           AKMALSMILQ FSFELSPSYTHTP   LTTQPQHGAHIILHKL
Sbjct: 481 AKMALSMILQRFSFELSPSYTHTPHTGLTTQPQHGAHIILHKL 522

BLAST of Cucsa.058110 vs. NCBI nr
Match: gi|778695569|ref|XP_011654017.1| (PREDICTED: cytochrome P450 CYP72A219 isoform X2 [Cucumis sativus])

HSP 1 Score: 847.8 bits (2189), Expect = 1.0e-242
Identity = 412/526 (78.33%), Postives = 464/526 (88.21%), Query Frame = 1

Query: 1   MWYVPHMIST-PIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILH 60
           M YV  MIST  IV+I  L+   LL LGWKL DWIWF P++LE+LLR+QGFTGNSY+ILH
Sbjct: 1   MAYVLDMISTWLIVVIVVLLSSSLLLLGWKLVDWIWFRPKKLEKLLRQQGFTGNSYRILH 60

Query: 61  GDMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQ 120
           GD+KE AAMR +A+SKPM FSN+IAPRVIPSV+HTIQ YGKNS MW+GP+PRVHIMDPEQ
Sbjct: 61  GDLKERAAMRDQAISKPMNFSNYIAPRVIPSVHHTIQHYGKNSFMWIGPMPRVHIMDPEQ 120

Query: 121 LKTVFSFFNDFQRPTT--DPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAF 180
           LKTVFS  ND+Q+PT   +P  +LL  GL++ EG+KWVKHRKIINPAFHLEKLK MVPAF
Sbjct: 121 LKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGQKWVKHRKIINPAFHLEKLKDMVPAF 180

Query: 181 YHSCNEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELT 240
           YHSC+EM+SKWESMVS EG CELDVMPYLQNM ADVISRTAFGSSYE+GKKIFKLQTEL 
Sbjct: 181 YHSCDEMISKWESMVSVEGFCELDVMPYLQNMTADVISRTAFGSSYEKGKKIFKLQTELG 240

Query: 241 NLVIQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLS 300
            +V+Q++L  YIPGWRFLPTKSN K+K  ++EI+TL++ IINE+E SMK+GE IQTDLLS
Sbjct: 241 EMVMQSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMDIINERETSMKSGEAIQTDLLS 300

Query: 301 ILMESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQE 360
           ILMESN+NEIK   N+KD+GMSI+ VI+EC+LFYIAGQETTATLL WTMVLLSSYS+WQE
Sbjct: 301 ILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQETTATLLTWTMVLLSSYSEWQE 360

Query: 361 RARAEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPG 420
           RARAEVFEIFGNKKPDYDGL+RLKVVTMI NEVLRLYPPVSM GR  +KETKLG +TLP 
Sbjct: 361 RARAEVFEIFGNKKPDYDGLSRLKVVTMILNEVLRLYPPVSMFGRLVKKETKLGKLTLPA 420

Query: 421 GIMLSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFA 480
           G+ML LP++L+Q DPELWGEDAHEF PERFSEGV KA KNPGAF+PFGWGPRICIG NFA
Sbjct: 421 GVMLGLPVVLMQCDPELWGEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFA 480

Query: 481 MIEAKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 524
           MIEAKMALSMILQ FSF+LSPSYTHTP A LTTQPQHGAHIILHKL
Sbjct: 481 MIEAKMALSMILQRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 526