Cucsa.054620 (gene) Cucumber (Gy14) v1

NameCucsa.054620
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionLeucine-rich receptor-like protein kinase family protein
Locationscaffold00585 : 191203 .. 193963 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGGAAAGGAGTGGGGTGTGATCATACCAATGGCGGTCATGTTGTCAAGCTTGATCTTCGAAATTATGAGTATTTCTATTCCTCTGCTTTCCTTAGCAATGGTGTGGACTCTAGTTTGTTTGAGTCGAAATATTTGAATTACTTGGGTTTAAGTGCAAATTTTTTCAACTACACTCCCATTCCTAATTCTTTTGCTGGGTTGTTGGGATTAACATATCTCAATTTATCTTCCACGTATTTTCATGGAGCAATTCAACCTTTTCTTGGAAATCTAACTAAATTGCTGGTTCTTGATTTCAACAACAAGGGGCAGTTGAATGGTCCACATAATTTTCCTTTGGGGGAGTTGTTTATTGATGGCCTTGCCTTTAGATGGATTTCTAGTCTTCGATGTGTAGAATACATCGACTTAAGTGGTGTAAGAGTAGTTCAGAGTGGAAAGTTGGATGTAGATTATTTGATCCAACTATTGAATTCAATTCCTTCTTGCTTCTCATTAAATCTGAGTTCTTGTGCTCTTCAAAACTACCAGCTTCTTGATGCTCCACTAAATTCTTCCTTTCGTTCCAAACTTCAACATCTAGATTTGTCTTATAACGAGTTTGATGGTCCAATTCCTATTATTCTTCGAAACATGACTTCTTTGAGATACCTAAACCTTAACGGTAATTTATTATAATTATGAAATCCTTTCTTGGTTAAGTAATCTTAGGAATCTTGATACTTTGAGTCTTGGATACAACTTGTTCAGTAGCATTGAAGGTGGTTTGTCCTCGATGGTTAGAAACAATTGTCATATGAAAAGTCTTGATTTATTATTTAACAATTTCGTTGGTAAAGATGTTTTTGGAAGTTATGAAATTTTTTCTTCTGGTTGTAACGAGTATGGTCTACAAAGGCTTTATCTAGAGGAGATGATTGGATTTGGGATGCATACAATTCCAAAGGCTTTATCTAGAGGAGATGATTGGATTTGGGATGCATACAATTCCAAGTTGGTTGGGAGAATTAAAAAGCTTGAAGTCTCTTTCTCTTCGAGGGAATGCACTTCATGGTCCAATTCCCTCCTCATTTGGAAATTTGTCAAGTCTTAAGACTTGGATATTTCTAATAACATGATTAGTGGAGGAATTCCAATCTCATTTGGACAACTTCGAAATCTCAATACGTTTGATGTTTCTAAGAATTCTCTCAAAGGTGTTATTACAGAAACACACTTTGCTAATCTCTCACAGTTGAAGATGGTAGCTGTGAGTAGCAATGAGCATCTATCTTTTGAAATAAAACATGATTGGGTCCCTCCTTTCCAATTACAATATTTTTGAGTGAGATCAACTAAGGGCTTTGGATCAAATGGATTTCATCGATGGTTGGTAACACAAAAAGATGAACTAGGTTTAGTTTTAGTTTTGTCAAACATCAGTCTTTGAGGACCTATACCAACATGGCTAAGCTTTCCAAATTTTATTTATTTGGACATTTCTAACAATCAAATTAGTGGACCACTCCCAGACAACATTGGCTATCAAATTCCTAATATTTTTGCCTTTTATATTTCCAACAACATGCATATCAATGGTTCCTTGCCACCATCTGTTTGCAAATGGAGGCATCTAGTATTTTTGGGACTTTCAAATAATGAATTATCAGGGACAATTCCTAGTTGTCTGTTGATACCAAACTTGACCGTTTTTTACTTATCATCAAACAAGTTCTCAGGGGTTTTTCCAAGTAATTCATTCTATAATATTTCTACACTTGAACTGATGAACTTGGCAAACAACAAACTTGAAGGAGAGTCACTAGTTGATGTGAGCAATTGCACATTCTTCAAGGAAACCAATTCTCTGGAAGTATTCCCTCATGGATGGGTAGAAGACTTCAAAGTTTGCAAATTTTGAATCTTCAAGGAAATTCATTCGACGACACTATTCCTTTTTCTCTATGGATATTGCCTCGCTTACAAATTTTGATTCTTGCAGACAACAAACTAGAAGGAGAGATCCCACCAATTGAGGCAAAGTTTGCAACCAAATTTGAAAAATCAACCGTTTCAGGTGTAATGTGCAACTCTAAAGAAGATCAATATGTAATTTGTTACATGAGCTACCTTAGACAAGTCATGAAATCAAACAATTTAAACGACTCTTACGTACATGTATATTCAATGGTGAATGTCGACCTCTCCAATAACAACTTGCAAGGACATATTCCAAGGGAAATAGTGATGATAAATAGTTTGTCTAACTTAAATTTGTCTCATAATAATCTATCAGGATCCATTCCTGTGGAGATTGGAAGATCATTAAGATTGGAATCCCTTGACCTCTCATCCAATCAACTCTCCGGATCAATCCCACTCAACATGGAAAAATTAAATTCACTTAGAGCTTTAAATTTGTCCAACAACAATTTCTCAGGACGCATACCTCGAGGAGGTCATCTTTCAACCTTTAAGGATGCATCCAGTTAGGAAGGGAATCCACATCTTTGTGGTGATCCATTTCCTAATACATGTCCAAATGAAAATGGTAACCATAGTGATGTAAAAGATAAATTGGAGACAATGTGGTTTTGTGTGATTGTGGTGGCTGGATATTCTTTCGGATTTTGGGGAGTTGTTGGAACTTTGATATTGAAGAAAAGTTGGAGACATGCTTACTTTCGGTTTATGGATGAAACGAAAGACAAGATGAGTGTTGCAATATTGGTGAATATGGCAAGGTTGAAACAACGAATGAGAGGAAATTACATGTTATAA

mRNA sequence

TTGGAAAGGAGTGGGGTGTGATCATACCAATGGCGGTCATGTTGTCAAGCTTGATCTTCGAAATTATGAGTATTTCTATTCCTCTGCTTTCCTTAGCAATGGTGTGGACTCTAGTTTGTTTGAGTCGAAATATTTGAATTACTTGGGTTTAAGTGCAAATTTTTTCAACTACACTCCCATTCCTAATTCTTTTGCTGGGTTGTTGGGATTAACATATCTCAATTTATCTTCCACGTATTTTCATGGAGCAATTCAACCTTTTCTTGGAAATCTAACTAAATTGCTGGTTCTTGATTTCAACAACAAGGGGCAGTTGAATGGTCCACATAATTTTCCTTTGGGGGAGTTGTTTATTGATGGCCTTGCCTTTAGATGGATTTCTAGTCTTCGATGTGTAGAATACATCGACTTAAGTGGTGTAAGAGTAGTTCAGAGTGGAAAGTTGGATGTAGATTATTTGATCCAACTATTGAATTCAATTCCTTCTTGCTTCTCATTAAATCTGAGTTCTTGTGCTCTTCAAAACTACCAGCTTCTTGATGCTCCACTAAATTCTTCCTTTCGTTCCAAACTTCAACATCTAGATTTGTCTTATAACGAGTTTGATGGTCCAATTCCTATTATTCTTCGAAACATGACTTCTTTGAGATACCTAAACCTTAACGGTGGTTTGTCCTCGATGGTTAGAAACAATTGTCATATGAAAAGTCTTGATTTATTATTTAACAATTTCGTTGAGGAGATGATTGGATTTGGGATGCATACAATTCCAAGTTGGTTGGGAGAATTAAAAAGCTTGAAGTCTCTTTCTCTTCGAGGGAATGCACTTCATGGTCCAATTCCCTCCTCATTTGGAAATTTGTCAAGAATTCCAATCTCATTTGGACAACTTCGAAATCTCAATACGTTTGATGTTTCTAAGAATTCTCTCAAAGGTGTTATTACAGAAACACACTTTGCTAATCTCTCACAGTTGAAGATGGTAGCTGTGAGTAGCAATGAGCATCTATCTTTTGAAATAAAACATGATTGGGGCTTTGGATCAAATGGATTTCATCGATGGTTGGTAACACAAAAAGATGAACTAGGACCTATACCAACATGGCTAAGCTTTCCAAATTTTATTTATTTGGACATTTCTAACAATCAAATTAGTGGACCACTCCCAGACAACATTGGCTATCAAATTCCTAATATTTTTGCCTTTTATATTTCCAACAACATGCATATCAATGGTTCCTTGCCACCATCTGTTTGCAAATGGAGGCATCTAGTATTTTTGGGACTTTCAAATAATGAATTATCAGGGACAATTCCTAGTTGTCTGTTGATACCAAACTTGACCGTTTTTTACTTATCATCAAACAAGTTCTCAGGGGTTTTTCCAAGAAACCAATTCTCTGGAAGTATTCCCTCATGGATGGGTAGAAGACTTCAAAGTTTGCAAATTTTGAATCTTCAAGGAAATTCATTCGACGACACTATTCCTTTTTCTCTATGGATATTGCCTCGCTTACAAATTTTGATTCTTGCAGACAACAAACTAGAAGGAGAGATCCCACCAATTGAGGCAAAGTTTGCAACCAAATTTGAAAAATCAACCGTTTCAGGTGTAATGTGCAACTCTAAAGAAGATCAATATGTAATTTGTTACATGAGCTACCTTAGACAAGTCATGAAATCAAACAATTTAAACGACTCTTACGTACATGTATATTCAATGGTGAATGTCGACCTCTCCAATAACAACTTGCAAGGACATATTCCAAGGGAAATAGTGATGATAAATAGTTTGTCTAACTTAAATTTGTCTCATAATAATCTATCAGGATCCATTCCTGTGGAGATTGGAAGATCATTAAGATTGGAATCCCTTGACCTCTCATCCAATCAACTCTCCGGATCAATCCCACTCAACATGGAAAAATTAAATTCACTTAGAGCTTTAAATTTGTCCAACAACAATTTCTCAGGACGCATACCTCGAGGAGGGAATCCACATCTTTGTGGTGATCCATTTCCTAATACATGTCCAAATGAAAATGGTAACCATAGTGATGTAAAAGATAAATTGGAGACAATGTGGTTTTGTGTGATTGTGGTGGCTGGATATTCTTTCGGATTTTGGGGAGTTGTTGGAACTTTGATATTGAAGAAAAGTTGGAGACATGCTTACTTTCGGTTTATGGATGAAACGAAAGACAAGATGAGTGTTGCAATATTGGTGAATATGGCAAGGTTGAAACAACGAATGAGAGGAAATTACATGTTATAA

Coding sequence (CDS)

TTGGAAAGGAGTGGGGTGTGATCATACCAATGGCGGTCATGTTGTCAAGCTTGATCTTCGAAATTATGAGTATTTCTATTCCTCTGCTTTCCTTAGCAATGGTGTGGACTCTAGTTTGTTTGAGTCGAAATATTTGAATTACTTGGGTTTAAGTGCAAATTTTTTCAACTACACTCCCATTCCTAATTCTTTTGCTGGGTTGTTGGGATTAACATATCTCAATTTATCTTCCACGTATTTTCATGGAGCAATTCAACCTTTTCTTGGAAATCTAACTAAATTGCTGGTTCTTGATTTCAACAACAAGGGGCAGTTGAATGGTCCACATAATTTTCCTTTGGGGGAGTTGTTTATTGATGGCCTTGCCTTTAGATGGATTTCTAGTCTTCGATGTGTAGAATACATCGACTTAAGTGGTGTAAGAGTAGTTCAGAGTGGAAAGTTGGATGTAGATTATTTGATCCAACTATTGAATTCAATTCCTTCTTGCTTCTCATTAAATCTGAGTTCTTGTGCTCTTCAAAACTACCAGCTTCTTGATGCTCCACTAAATTCTTCCTTTCGTTCCAAACTTCAACATCTAGATTTGTCTTATAACGAGTTTGATGGTCCAATTCCTATTATTCTTCGAAACATGACTTCTTTGAGATACCTAAACCTTAACGGTGGTTTGTCCTCGATGGTTAGAAACAATTGTCATATGAAAAGTCTTGATTTATTATTTAACAATTTCGTTGAGGAGATGATTGGATTTGGGATGCATACAATTCCAAGTTGGTTGGGAGAATTAAAAAGCTTGAAGTCTCTTTCTCTTCGAGGGAATGCACTTCATGGTCCAATTCCCTCCTCATTTGGAAATTTGTCAAGAATTCCAATCTCATTTGGACAACTTCGAAATCTCAATACGTTTGATGTTTCTAAGAATTCTCTCAAAGGTGTTATTACAGAAACACACTTTGCTAATCTCTCACAGTTGAAGATGGTAGCTGTGAGTAGCAATGAGCATCTATCTTTTGAAATAAAACATGATTGGGGCTTTGGATCAAATGGATTTCATCGATGGTTGGTAACACAAAAAGATGAACTAGGACCTATACCAACATGGCTAAGCTTTCCAAATTTTATTTATTTGGACATTTCTAACAATCAAATTAGTGGACCACTCCCAGACAACATTGGCTATCAAATTCCTAATATTTTTGCCTTTTATATTTCCAACAACATGCATATCAATGGTTCCTTGCCACCATCTGTTTGCAAATGGAGGCATCTAGTATTTTTGGGACTTTCAAATAATGAATTATCAGGGACAATTCCTAGTTGTCTGTTGATACCAAACTTGACCGTTTTTTACTTATCATCAAACAAGTTCTCAGGGGTTTTTCCAAGAAACCAATTCTCTGGAAGTATTCCCTCATGGATGGGTAGAAGACTTCAAAGTTTGCAAATTTTGAATCTTCAAGGAAATTCATTCGACGACACTATTCCTTTTTCTCTATGGATATTGCCTCGCTTACAAATTTTGATTCTTGCAGACAACAAACTAGAAGGAGAGATCCCACCAATTGAGGCAAAGTTTGCAACCAAATTTGAAAAATCAACCGTTTCAGGTGTAATGTGCAACTCTAAAGAAGATCAATATGTAATTTGTTACATGAGCTACCTTAGACAAGTCATGAAATCAAACAATTTAAACGACTCTTACGTACATGTATATTCAATGGTGAATGTCGACCTCTCCAATAACAACTTGCAAGGACATATTCCAAGGGAAATAGTGATGATAAATAGTTTGTCTAACTTAAATTTGTCTCATAATAATCTATCAGGATCCATTCCTGTGGAGATTGGAAGATCATTAAGATTGGAATCCCTTGACCTCTCATCCAATCAACTCTCCGGATCAATCCCACTCAACATGGAAAAATTAAATTCACTTAGAGCTTTAAATTTGTCCAACAACAATTTCTCAGGACGCATACCTCGAGGAGGGAATCCACATCTTTGTGGTGATCCATTTCCTAATACATGTCCAAATGAAAATGGTAACCATAGTGATGTAAAAGATAAATTGGAGACAATGTGGTTTTGTGTGATTGTGGTGGCTGGATATTCTTTCGGATTTTGGGGAGTTGTTGGAACTTTGATATTGAAGAAAAGTTGGAGACATGCTTACTTTCGGTTTATGGATGAAACGAAAGACAAGATGAGTGTTGCAATATTGGTGAATATGGCAAGGTTGAAACAACGAATGAGAGGAAATTACATGTTATAA

Protein sequence

WKGVGCDHTNGGHVVKLDLRNYEYFYSSAFLSNGVDSSLFESKYLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLNGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLLIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIPRGGNPHLCGDPFPNTCPNENGNHSDVKDKLETMWFCVIVVAGYSFGFWGVVGTLILKKSWRHAYFRFMDETKDKMSVAILVNMARLKQRMRGNYML*
BLAST of Cucsa.054620 vs. Swiss-Prot
Match: GSO1_ARATH (LRR receptor-like serine/threonine-protein kinase GSO1 OS=Arabidopsis thaliana GN=GSO1 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 2.8e-52
Identity = 189/639 (29.58%), Postives = 296/639 (46.32%), Query Frame = 1

Query: 53  NFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFP 112
           N+ ++T +     GL  +  LNL+     G+I P+ G    L+ LD ++   L GP    
Sbjct: 56  NYCSWTGVTCDNTGLFRVIALNLTGLGLTGSISPWFGRFDNLIHLDLSSNN-LVGPIPTA 115

Query: 113 LGELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLI----QLLNSIPSCFSLNL 172
           L  L      F + + L       L  +  ++S ++  + L+    + L ++ +   L L
Sbjct: 116 LSNLTSLESLFLFSNQLTGEIPSQLGSLVNIRSLRIGDNELVGDIPETLGNLVNLQMLAL 175

Query: 173 SSCALQNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLN-----LNGG 232
           +SC L        P       ++Q L L  N  +GPIP  L N + L         LNG 
Sbjct: 176 ASCRLTG----PIPSQLGRLVRVQSLILQDNYLEGPIPAELGNCSDLTVFTAAENMLNGT 235

Query: 233 LSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSS 292
           + + +    +++ L+L  N+   E        IPS LGE+  L+ LSL  N L G IP S
Sbjct: 236 IPAELGRLENLEILNLANNSLTGE--------IPSQLGEMSQLQYLSLMANQLQGLIPKS 295

Query: 293 FGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHD 352
             +L           NL T D+S N+L G I E  F N+SQL +  V +N HLS  +   
Sbjct: 296 LADLG----------NLQTLDLSANNLTGEIPE-EFWNMSQL-LDLVLANNHLSGSLPKS 355

Query: 353 WGFGSNGFHRWLVTQKDELGPIPTWLS-FPNFIYLDISNNQISGPLPDNIGYQIPNIFAF 412
               +    + +++     G IP  LS   +   LD+SNN ++G +P+ + +++  +   
Sbjct: 356 ICSNNTNLEQLVLSGTQLSGEIPVELSKCQSLKQLDLSNNSLAGSIPEAL-FELVELTDL 415

Query: 413 YISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCL-LIPNLTVFYLSSNKFSGVF 472
           Y+ NN  + G+L PS+    +L +L L +N L G +P  +  +  L V +L  N+FSG  
Sbjct: 416 YLHNNT-LEGTLSPSISNLTNLQWLVLYHNNLEGKLPKEISALRKLEVLFLYENRFSGEI 475

Query: 473 PR---------------NQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQ 532
           P+               N F G IP  +GR L+ L +L+L+ N     +P SL    +L 
Sbjct: 476 PQEIGNCTSLKMIDMFGNHFEGEIPPSIGR-LKELNLLHLRQNELVGGLPASLGNCHQLN 535

Query: 533 ILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLND 592
           IL LADN+L G IP     F    E+  +            +I   +  R  +  N LN 
Sbjct: 536 ILDLADNQLSGSIPS-SFGFLKGLEQLMLYNNSLQGNLPDSLISLRNLTRINLSHNRLNG 595

Query: 593 SYVHVY----SMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRL 652
           + +H      S ++ D++NN  +  IP E+    +L  L L  N L+G IP  +G+   L
Sbjct: 596 T-IHPLCGSSSYLSFDVTNNGFEDEIPLELGNSQNLDRLRLGKNQLTGKIPWTLGKIREL 655

Query: 653 ESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIP 662
             LD+SSN L+G+IPL +     L  ++L+NN  SG IP
Sbjct: 656 SLLDMSSNALTGTIPLQLVLCKKLTHIDLNNNFLSGPIP 664


HSP 2 Score: 179.5 bits (454), Expect = 1.4e-43
Identity = 202/744 (27.15%), Postives = 312/744 (41.94%), Query Frame = 1

Query: 31  LSNGVDSSLFESKYLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGN 90
           L+  + S L E   L YL L AN      IP S A L  L  L+LS+    G I     N
Sbjct: 251 LTGEIPSQLGEMSQLQYLSLMANQLQGL-IPKSLADLGNLQTLDLSANNLTGEIPEEFWN 310

Query: 91  LTKLLVLDFNNKGQLNGP-------HNFPLGELFIDGLAFRW-----ISSLRCVEYIDLS 150
           +++LL L   N   L+G        +N  L +L + G          +S  + ++ +DLS
Sbjct: 311 MSQLLDLVLANN-HLSGSLPKSICSNNTNLEQLVLSGTQLSGEIPVELSKCQSLKQLDLS 370

Query: 151 GVRVVQSGKLDVDYLIQLLNSIPSCFS--LNLSSCALQNYQLLDAPLNSSFRS--KLQHL 210
                            L  SIP      + L+   L N   L+  L+ S  +   LQ L
Sbjct: 371 NN--------------SLAGSIPEALFELVELTDLYLHN-NTLEGTLSPSISNLTNLQWL 430

Query: 211 DLSYNEFDGPIPIILRNMTSLRYLNL-----NGGLSSMVRNNCHMKSLDLLFNNFVEEMI 270
            L +N  +G +P  +  +  L  L L     +G +   + N   +K +D+  N+F  E  
Sbjct: 431 VLYHNNLEGKLPKEISALRKLEVLFLYENRFSGEIPQEIGNCTSLKMIDMFGNHFEGE-- 490

Query: 271 GFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSR--------------IPISFG 330
                 IP  +G LK L  L LR N L G +P+S GN  +              IP SFG
Sbjct: 491 ------IPPSIGRLKELNLLHLRQNELVGGLPASLGNCHQLNILDLADNQLSGSIPSSFG 550

Query: 331 QLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWL 390
            L+ L    +  NSL+G + ++   +L  L  + +S N                      
Sbjct: 551 FLKGLEQLMLYNNSLQGNLPDS-LISLRNLTRINLSHNRLN------------------- 610

Query: 391 VTQKDELGPIPTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLP 450
                  G I       +++  D++NN     +P  +G    N+    +  N  + G +P
Sbjct: 611 -------GTIHPLCGSSSYLSFDVTNNGFEDEIPLELGNS-QNLDRLRLGKNQ-LTGKIP 670

Query: 451 PSVCKWRHLVFLGLSNNELSGTIPSCLLIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMG 510
            ++ K R L  L +S+N L+GTIP  L++         +N F         SG IP W+G
Sbjct: 671 WTLGKIRELSLLDMSSNALTGTIPLQLVLCKKLTHIDLNNNF--------LSGPIPPWLG 730

Query: 511 RRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTV 570
           + L  L  L L  N F +++P  L+   +L +L L  N L G IP          E   +
Sbjct: 731 K-LSQLGELKLSSNQFVESLPTELFNCTKLLVLSLDGNSLNGSIPQ---------EIGNL 790

Query: 571 SGV-MCNSKEDQYVICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVM 630
             + + N  ++Q+             S +L  +   +  +  + LS N+L G IP EI  
Sbjct: 791 GALNVLNLDKNQF-------------SGSLPQAMGKLSKLYELRLSRNSLTGEIPVEIGQ 850

Query: 631 INSL-SNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSN 690
           +  L S L+LS+NN +G IP  IG   +LE+LDLS NQL+G +P ++  + SL  LN+S 
Sbjct: 851 LQDLQSALDLSYNNFTGDIPSTIGTLSKLETLDLSHNQLTGEVPGSVGDMKSLGYLNVSF 906

Query: 691 NNFSGRIPRG----------GNPHLCGDPFPNTCPNENGNHSDVKDKLETMWFCVIVVAG 728
           NN  G++ +           GN  LCG P  + C     N+        ++     + A 
Sbjct: 911 NNLGGKLKKQFSRWPADSFLGNTGLCGSPL-SRCNRVRSNNKQQGLSARSVVIISAISAL 906


HSP 3 Score: 173.7 bits (439), Expect = 7.6e-42
Identity = 184/652 (28.22%), Postives = 283/652 (43.40%), Query Frame = 1

Query: 59  PIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFI 118
           PIP+    L+ +  L L   Y  G I   LGN + L V     +  LNG     LG L  
Sbjct: 182 PIPSQLGRLVRVQSLILQDNYLEGPIPAELGNCSDLTVFTAA-ENMLNGTIPAELGRL-- 241

Query: 119 DGLAFRWISSLRCVEYIDLSGVRVVQSGKLD-VDYLIQLLNSIPSCFSLNLSSCALQNYQ 178
           + L    +++        L+G    Q G++  + YL  + N +      +L+   L N Q
Sbjct: 242 ENLEILNLANN------SLTGEIPSQLGEMSQLQYLSLMANQLQGLIPKSLAD--LGNLQ 301

Query: 179 LLDAPLNSSF---------RSKLQHLDLSYNEFDGPIP-IILRNMTSLRYLNLNG----G 238
            LD   N+            S+L  L L+ N   G +P  I  N T+L  L L+G    G
Sbjct: 302 TLDLSANNLTGEIPEEFWNMSQLLDLVLANNHLSGSLPKSICSNNTNLEQLVLSGTQLSG 361

Query: 239 LSSMVRNNCH-MKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPS 298
              +  + C  +K LDL  N+           +IP  L EL  L  L L  N L G +  
Sbjct: 362 EIPVELSKCQSLKQLDLSNNSLAG--------SIPEALFELVELTDLYLHNNTLEGTLSP 421

Query: 299 SFGNLS--------------RIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMV 358
           S  NL+              ++P     LR L    + +N   G I +    N + LKM+
Sbjct: 422 SISNLTNLQWLVLYHNNLEGKLPKEISALRKLEVLFLYENRFSGEIPQ-EIGNCTSLKMI 481

Query: 359 AVSSNEHLSFEIKHDWGFGS--NGFHRWLVTQKDELGPIPTWLSFPNFI-YLDISNNQIS 418
            +  N H   EI    G     N  H   + Q + +G +P  L   + +  LD+++NQ+S
Sbjct: 482 DMFGN-HFEGEIPPSIGRLKELNLLH---LRQNELVGGLPASLGNCHQLNILDLADNQLS 541

Query: 419 GPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLLIP 478
           G +P + G+ +  +    + NN  + G+LP S+   R+L  + LS+N L+GTI       
Sbjct: 542 GSIPSSFGF-LKGLEQLMLYNNS-LQGNLPDSLISLRNLTRINLSHNRLNGTIHPLCGSS 601

Query: 479 NLTVFYLSSNKFSGVFP---------------RNQFSGSIPSWMGRRLQSLQILNLQGNS 538
           +   F +++N F    P               +NQ +G IP W   +++ L +L++  N+
Sbjct: 602 SYLSFDVTNNGFEDEIPLELGNSQNLDRLRLGKNQLTGKIP-WTLGKIRELSLLDMSSNA 661

Query: 539 FDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVIC 598
              TIP  L +  +L  + L +N L G IPP   K +   E    S     S   +   C
Sbjct: 662 LTGTIPLQLVLCKKLTHIDLNNNFLSGPIPPWLGKLSQLGELKLSSNQFVESLPTELFNC 721

Query: 599 YMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSG 658
                                  ++ + L  N+L G IP+EI  + +L+ LNL  N  SG
Sbjct: 722 T---------------------KLLVLSLDGNSLNGSIPQEIGNLGALNVLNLDKNQFSG 781

Query: 659 SIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLR-ALNLSNNNFSGRIP 662
           S+P  +G+  +L  L LS N L+G IP+ + +L  L+ AL+LS NNF+G IP
Sbjct: 782 SLPQAMGKLSKLYELRLSRNSLTGEIPVEIGQLQDLQSALDLSYNNFTGDIP 785

BLAST of Cucsa.054620 vs. Swiss-Prot
Match: GSO2_ARATH (LRR receptor-like serine/threonine-protein kinase GSO2 OS=Arabidopsis thaliana GN=GSO2 PE=2 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 3.1e-51
Identity = 192/640 (30.00%), Postives = 299/640 (46.72%), Query Frame = 1

Query: 51  SANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHN 110
           S ++ N+T +      ++GL   NLS     G+I P +G    L+ +D ++  +L GP  
Sbjct: 57  SPSYCNWTGVTCGGREIIGL---NLSGLGLTGSISPSIGRFNNLIHIDLSSN-RLVGPIP 116

Query: 111 FPLGELFIDGLAFRWISSLRCVEYIDLSGVRV-VQSGKLDVDYLIQLLNSIPSCFS--LN 170
             L  L     +    S+L   +     G  V ++S KL  +   +L  +IP  F   +N
Sbjct: 117 TTLSNLSSSLESLHLFSNLLSGDIPSQLGSLVNLKSLKLGDN---ELNGTIPETFGNLVN 176

Query: 171 LSSCALQNYQLLDA-PLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLR-----YLNLN 230
           L   AL + +L    P       +LQ L L  NE +GPIP  + N TSL      +  LN
Sbjct: 177 LQMLALASCRLTGLIPSRFGRLVQLQTLILQDNELEGPIPAEIGNCTSLALFAAAFNRLN 236

Query: 231 GGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIP 290
           G L + +    ++++L+L  N+F  E        IPS LG+L S++ L+L GN L G IP
Sbjct: 237 GSLPAELNRLKNLQTLNLGDNSFSGE--------IPSQLGDLVSIQYLNLIGNQLQGLIP 296

Query: 291 SSFGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIK 350
                L+          NL T D+S N+L GVI E  F  ++QL+ + ++ N  LS  + 
Sbjct: 297 KRLTELA----------NLQTLDLSSNNLTGVIHE-EFWRMNQLEFLVLAKNR-LSGSLP 356

Query: 351 HDWGFGSNGFHRWLVTQKDELGPIPTWLS-FPNFIYLDISNNQISGPLPDNIGYQIPNIF 410
                 +    +  +++    G IP  +S   +   LD+SNN ++G +PD++ +Q+  + 
Sbjct: 357 KTICSNNTSLKQLFLSETQLSGEIPAEISNCQSLKLLDLSNNTLTGQIPDSL-FQLVELT 416

Query: 411 AFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCL-LIPNLTVFYLSSNKFSG 470
             Y++NN  + G+L  S+    +L    L +N L G +P  +  +  L + YL  N+FSG
Sbjct: 417 NLYLNNNS-LEGTLSSSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKLEIMYLYENRFSG 476

Query: 471 VFP---------------RNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPR 530
             P                N+ SG IPS +GR L+ L  L+L+ N     IP SL    +
Sbjct: 477 EMPVEIGNCTRLQEIDWYGNRLSGEIPSSIGR-LKDLTRLHLRENELVGNIPASLGNCHQ 536

Query: 531 LQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNL 590
           + ++ LADN+L G IP     F T  E   +            +I   +  R    SN  
Sbjct: 537 MTVIDLADNQLSGSIPS-SFGFLTALELFMIYNNSLQGNLPDSLINLKNLTRINFSSNKF 596

Query: 591 NDSYVHVY---SMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLR 650
           N S   +    S ++ D++ N  +G IP E+    +L  L L  N  +G IP   G+   
Sbjct: 597 NGSISPLCGSSSYLSFDVTENGFEGDIPLELGKSTNLDRLRLGKNQFTGRIPRTFGKISE 656

Query: 651 LESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIP 662
           L  LD+S N LSG IP+ +     L  ++L+NN  SG IP
Sbjct: 657 LSLLDISRNSLSGIIPVELGLCKKLTHIDLNNNYLSGVIP 665


HSP 2 Score: 166.4 bits (420), Expect = 1.2e-39
Identity = 197/690 (28.55%), Postives = 290/690 (42.03%), Query Frame = 1

Query: 32  SNGVDSSLFESKYLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNL 91
           S  + S L +   + YL L  N      IP     L  L  L+LSS    G I      +
Sbjct: 253 SGEIPSQLGDLVSIQYLNLIGNQLQGL-IPKRLTELANLQTLDLSSNNLTGVIHEEFWRM 312

Query: 92  TKLLVLDFNNKGQLNGP-------HNFPLGELFID-----GLAFRWISSLRCVEYIDLSG 151
            +L  L    K +L+G        +N  L +LF+      G     IS+ + ++ +DLS 
Sbjct: 313 NQLEFLVLA-KNRLSGSLPKTICSNNTSLKQLFLSETQLSGEIPAEISNCQSLKLLDLSN 372

Query: 152 VRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLLDAPLNSSFRS--KLQHLDLS 211
             +  +G++  D L QL+          L++  L N  L +  L+SS  +   LQ   L 
Sbjct: 373 NTL--TGQIP-DSLFQLVE---------LTNLYLNNNSL-EGTLSSSISNLTNLQEFTLY 432

Query: 212 YNEFDGPIPIILRNMTSLRYLNL-----NGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFG 271
           +N  +G +P  +  +  L  + L     +G +   + N   ++ +D   N    E     
Sbjct: 433 HNNLEGKVPKEIGFLGKLEIMYLYENRFSGEMPVEIGNCTRLQEIDWYGNRLSGE----- 492

Query: 272 MHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSR--------------IPISFGQLR 331
              IPS +G LK L  L LR N L G IP+S GN  +              IP SFG L 
Sbjct: 493 ---IPSSIGRLKDLTRLHLRENELVGNIPASLGNCHQMTVIDLADNQLSGSIPSSFGFLT 552

Query: 332 NLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQ 391
            L  F +  NSL+G + ++   NL  L  +                 F SN F+      
Sbjct: 553 ALELFMIYNNSLQGNLPDS-LINLKNLTRI----------------NFSSNKFN------ 612

Query: 392 KDELGPIPTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSV 451
               G I       +++  D++ N   G +P  +G +  N+    +  N    G +P + 
Sbjct: 613 ----GSISPLCGSSSYLSFDVTENGFEGDIPLELG-KSTNLDRLRLGKNQ-FTGRIPRTF 672

Query: 452 CKWRHLVFLGLSNNELSGTIPSCL-LIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMGRR 511
            K   L  L +S N LSG IP  L L   LT   L++N  SGV         IP+W+G+ 
Sbjct: 673 GKISELSLLDISRNSLSGIIPVELGLCKKLTHIDLNNNYLSGV---------IPTWLGK- 732

Query: 512 LQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPP----IEAKFATKFEKS 571
           L  L  L L  N F  ++P  ++ L  +  L L  N L G IP     ++A  A   E++
Sbjct: 733 LPLLGELKLSSNKFVGSLPTEIFSLTNILTLFLDGNSLNGSIPQEIGNLQALNALNLEEN 792

Query: 572 TVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIV 631
            +SG +             S + ++ K             +  + LS N L G IP EI 
Sbjct: 793 QLSGPL------------PSTIGKLSK-------------LFELRLSRNALTGEIPVEIG 852

Query: 632 MINSL-SNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLS 673
            +  L S L+LS+NN +G IP  I    +LESLDLS NQL G +P  +  + SL  LNLS
Sbjct: 853 QLQDLQSALDLSYNNFTGRIPSTISTLPKLESLDLSHNQLVGEVPGQIGDMKSLGYLNLS 855


HSP 3 Score: 165.2 bits (417), Expect = 2.7e-39
Identity = 177/654 (27.06%), Postives = 285/654 (43.58%), Query Frame = 1

Query: 60  IPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLV-------LDFNNKGQLNGPHNFP 119
           IP+ F  L+ L  L L      G I   +GN T L +       L+ +   +LN   N  
Sbjct: 184 IPSRFGRLVQLQTLILQDNELEGPIPAEIGNCTSLALFAAAFNRLNGSLPAELNRLKNLQ 243

Query: 120 ---LGELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLS 179
              LG+    G     +  L  ++Y++L G ++          + + L  + +  +L+LS
Sbjct: 244 TLNLGDNSFSGEIPSQLGDLVSIQYLNLIGNQLQ-------GLIPKRLTELANLQTLDLS 303

Query: 180 SCALQNYQLLDAPLNSSF--RSKLQHLDLSYNEFDGPIP-IILRNMTSLRYL-----NLN 239
           S        L   ++  F   ++L+ L L+ N   G +P  I  N TSL+ L      L+
Sbjct: 304 S------NNLTGVIHEEFWRMNQLEFLVLAKNRLSGSLPKTICSNNTSLKQLFLSETQLS 363

Query: 240 GGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIP 299
           G + + + N   +K LDL  N    +        IP  L +L  L +L L  N+L G + 
Sbjct: 364 GEIPAEISNCQSLKLLDLSNNTLTGQ--------IPDSLFQLVELTNLYLNNNSLEGTLS 423

Query: 300 SSFGNLS--------------RIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKM 359
           SS  NL+              ++P   G L  L    + +N   G +      N ++L+ 
Sbjct: 424 SSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKLEIMYLYENRFSGEMP-VEIGNCTRLQE 483

Query: 360 VAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWL-SFPNFIYLDISNNQISG 419
           +    N  LS EI    G       R  + + + +G IP  L +      +D+++NQ+SG
Sbjct: 484 IDWYGNR-LSGEIPSSIG-RLKDLTRLHLRENELVGNIPASLGNCHQMTVIDLADNQLSG 543

Query: 420 PLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLLIPN 479
            +P + G+ +  +  F I NN  + G+LP S+   ++L  +  S+N+ +G+I       +
Sbjct: 544 SIPSSFGF-LTALELFMIYNNS-LQGNLPDSLINLKNLTRINFSSNKFNGSISPLCGSSS 603

Query: 480 LTVFYLSSNKFSGVFP---------------RNQFSGSIPSWMGRRLQSLQILNLQGNSF 539
              F ++ N F G  P               +NQF+G IP   G+ +  L +L++  NS 
Sbjct: 604 YLSFDVTENGFEGDIPLELGKSTNLDRLRLGKNQFTGRIPRTFGK-ISELSLLDISRNSL 663

Query: 540 DDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICY 599
              IP  L +  +L  + L +N L G IP    K     E    S     S   +     
Sbjct: 664 SGIIPVELGLCKKLTHIDLNNNYLSGVIPTWLGKLPLLGELKLSSNKFVGSLPTE----- 723

Query: 600 MSYLRQVMKSNNLNDSYVHVYSMVNV---DLSNNNLQGHIPREIVMINSLSNLNLSHNNL 659
                              ++S+ N+    L  N+L G IP+EI  + +L+ LNL  N L
Sbjct: 724 -------------------IFSLTNILTLFLDGNSLNGSIPQEIGNLQALNALNLEENQL 783

Query: 660 SGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLR-ALNLSNNNFSGRIP 662
           SG +P  IG+  +L  L LS N L+G IP+ + +L  L+ AL+LS NNF+GRIP
Sbjct: 784 SGPLPSTIGKLSKLFELRLSRNALTGEIPVEIGQLQDLQSALDLSYNNFTGRIP 786

BLAST of Cucsa.054620 vs. Swiss-Prot
Match: Y1341_ARATH (Probable LRR receptor-like serine/threonine-protein kinase At1g34110 OS=Arabidopsis thaliana GN=At1g34110 PE=2 SV=2)

HSP 1 Score: 193.4 bits (490), Expect = 9.3e-48
Identity = 209/689 (30.33%), Postives = 314/689 (45.57%), Query Frame = 1

Query: 54  FFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPL 113
           F N + IP+  + L  L +LNLSST   G I P  G LT L +LD ++   L+GP    L
Sbjct: 78  FLNLSSIPD-LSSLSSLQFLNLSSTNLSGPIPPSFGKLTHLRLLDLSSNS-LSGPIPSEL 137

Query: 114 GELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLI----QLLN-SIPSCFSLNL 173
           G L    L F  +++ +      LSG    Q   L    ++     LLN SIPS F    
Sbjct: 138 GRL--STLQFLILNANK------LSGSIPSQISNLFALQVLCLQDNLLNGSIPSSFG--- 197

Query: 174 SSCALQNYQL-----LDAPLNSS--FRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNL- 233
           S  +LQ ++L     L  P+ +   F   L  L  + +   G IP    N+ +L+ L L 
Sbjct: 198 SLVSLQQFRLGGNTNLGGPIPAQLGFLKNLTTLGFAASGLSGSIPSTFGNLVNLQTLALY 257

Query: 234 ----NGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNAL 293
               +G +   +     +++L L  N            +IP  LG+L+ + SL L GN+L
Sbjct: 258 DTEISGTIPPQLGLCSELRNLYLHMNKLTG--------SIPKELGKLQKITSLLLWGNSL 317

Query: 294 HGPIPSSFGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHL 353
            G IP    N S          +L  FDVS N L G I       L  L+ + +S N   
Sbjct: 318 SGVIPPEISNCS----------SLVVFDVSANDLTGDIPGD-LGKLVWLEQLQLSDNMFT 377

Query: 354 SFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLS-FPNFIYLDISNNQISGPLPDNIGYQ 413
                                     G IP  LS   + I L +  N++SG +P  IG  
Sbjct: 378 --------------------------GQIPWELSNCSSLIALQLDKNKLSGSIPSQIG-N 437

Query: 414 IPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLL-IPNLTVFYLSS 473
           + ++ +F++  N  I+G++P S      LV L LS N+L+G IP  L  +  L+   L  
Sbjct: 438 LKSLQSFFLWENS-ISGTIPSSFGNCTDLVALDLSRNKLTGRIPEELFSLKRLSKLLLLG 497

Query: 474 NKFSGVFPR---------------NQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSL 533
           N  SG  P+               NQ SG IP  +G  LQ+L  L+L  N F   +P+ +
Sbjct: 498 NSLSGGLPKSVAKCQSLVRLRVGENQLSGQIPKEIGE-LQNLVFLDLYMNHFSGGLPYEI 557

Query: 534 WILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICY--MSYLRQ 593
             +  L++L + +N + G+IP  +       E+  +S    NS      + +  +SYL +
Sbjct: 558 SNITVLELLDVHNNYITGDIPA-QLGNLVNLEQLDLSR---NSFTGNIPLSFGNLSYLNK 617

Query: 594 VMKSNNLNDSYV-----HVYSMVNVDLSNNNLQGHIPREIVMINSLS-NLNLSHNNLSGS 653
           ++ +NNL    +     ++  +  +DLS N+L G IP+E+  + SL+ NL+LS+N  +G+
Sbjct: 618 LILNNNLLTGQIPKSIKNLQKLTLLDLSYNSLSGEIPQELGQVTSLTINLDLSYNTFTGN 677

Query: 654 IPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIPRG-------- 689
           IP       +L+SLDLSSN L G I + +  L SL +LN+S NNFSG IP          
Sbjct: 678 IPETFSDLTQLQSLDLSSNSLHGDIKV-LGSLTSLASLNISCNNFSGPIPSTPFFKTIST 700


HSP 2 Score: 49.7 bits (117), Expect = 1.7e-04
Identity = 63/232 (27.16%), Postives = 100/232 (43.10%), Query Frame = 1

Query: 31  LSNGVDSSLFESKYLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGN 90
           LS G+  S+ + + L  L +  N  +   IP     L  L +L+L   +F G +   + N
Sbjct: 440 LSGGLPKSVAKCQSLVRLRVGENQLS-GQIPKEIGELQNLVFLDLYMNHFSGGLPYEISN 499

Query: 91  LTKLLVLDFNNKGQLNGPHNFPLGELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKL-- 150
           +T L +LD +N               +I G     + +L  +E +DLS      +  L  
Sbjct: 500 ITVLELLDVHNN--------------YITGDIPAQLGNLVNLEQLDLSRNSFTGNIPLSF 559

Query: 151 -DVDYLIQL-LNSIPSCFSLNLSSCALQNYQLLDAPLNS----------SFRSKLQHLDL 210
            ++ YL +L LN+      +  S   LQ   LLD   NS             S   +LDL
Sbjct: 560 GNLSYLNKLILNNNLLTGQIPKSIKNLQKLTLLDLSYNSLSGEIPQELGQVTSLTINLDL 619

Query: 211 SYNEFDGPIPIILRNMTSLRYLNLNG----GLSSMVRNNCHMKSLDLLFNNF 245
           SYN F G IP    ++T L+ L+L+     G   ++ +   + SL++  NNF
Sbjct: 620 SYNTFTGNIPETFSDLTQLQSLDLSSNSLHGDIKVLGSLTSLASLNISCNNF 656

BLAST of Cucsa.054620 vs. Swiss-Prot
Match: FLS2_ARATH (LRR receptor-like serine/threonine-protein kinase FLS2 OS=Arabidopsis thaliana GN=FLS2 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 6.7e-46
Identity = 206/703 (29.30%), Postives = 310/703 (44.10%), Query Frame = 1

Query: 13  HVVKLDLRNYEYFYSSAFLSNGVDSSLFESKYLNYLGLSANFFNYT-PIPNSFAGLLGLT 72
           ++  LDLRN         LS  V   + ++  L  +G   N  N T  IP     L+ L 
Sbjct: 145 NIFYLDLRNN-------LLSGDVPEEICKTSSLVLIGFDYN--NLTGKIPECLGDLVHLQ 204

Query: 73  YLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDGLAFRWISSLRC 132
               +  +  G+I   +G L  L  LD +   QL G      G L         + SL  
Sbjct: 205 MFVAAGNHLTGSIPVSIGTLANLTDLDLSGN-QLTGKIPRDFGNLLN-------LQSLVL 264

Query: 133 VEYIDLSGVRVVQSG------KLDVDYLIQLLNSIPSCFSLNLSSCALQNYQ--LLDAPL 192
            E + L G    + G      +L++ Y  QL   IP+     +   AL+ Y+  L  +  
Sbjct: 265 TENL-LEGDIPAEIGNCSSLVQLEL-YDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIP 324

Query: 193 NSSFR-SKLQHLDLSYNEFDGPIPIILRNMTSLRYL-----NLNGGLSSMVRNNCHMKSL 252
           +S FR ++L HL LS N   GPI   +  + SL  L     N  G     + N  ++  L
Sbjct: 325 SSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVL 384

Query: 253 DLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSRIPISFGQL 312
            + FNN   E+        P+ LG L +L++LS   N L GPIPSS  N +         
Sbjct: 385 TVGFNNISGEL--------PADLGLLTNLRNLSAHDNLLTGPIPSSISNCT--------- 444

Query: 313 RNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVT 372
             L   D+S N + G I    F  ++ L  +++  N H + EI  D  F  +      V 
Sbjct: 445 -GLKLLDLSHNQMTGEIPR-GFGRMN-LTFISIGRN-HFTGEIPDDI-FNCSNLETLSVA 504

Query: 373 QKDELGPI-PTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPP 432
             +  G + P          L +S N ++GP+P  IG  + ++   Y+ +N    G +P 
Sbjct: 505 DNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIG-NLKDLNILYLHSN-GFTGRIPR 564

Query: 433 SVCKWRHLVFLGLSNNELSGTIPSCLL-IPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMG 492
            +     L  L + +N+L G IP  +  +  L+V  LS+NKFSG  P           + 
Sbjct: 565 EMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPA----------LF 624

Query: 493 RRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPP------IEAKFATK 552
            +L+SL  L+LQGN F+ +IP SL  L  L    ++DN L G IP          +    
Sbjct: 625 SKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLN 684

Query: 553 FEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLND-----SYVHVYSMVNVDLSNNNL 612
           F  + ++G +   KE    +  +  ++++  SNNL       S     ++  +D S NNL
Sbjct: 685 FSNNLLTGTI--PKE----LGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNL 744

Query: 613 QGHIPREIVM-INSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKL 672
            GHIP E+   ++ + +LNLS N+ SG IP   G    L SLDLSSN L+G IP ++  L
Sbjct: 745 SGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANL 788

Query: 673 NSLRALNLSNNNFSGRIPRG------------GNPHLCGDPFP 675
           ++L+ L L++NN  G +P              GN  LCG   P
Sbjct: 805 STLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKP 788


HSP 2 Score: 172.2 bits (435), Expect = 2.2e-41
Identity = 195/719 (27.12%), Postives = 301/719 (41.86%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDL-------------RNYEYFYSSAFLSNG----VDSSLFESK 60
           W G+ CD T  GHVV + L              N  Y       SN     + + + +  
Sbjct: 63  WTGITCDST--GHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLT 122

Query: 61  YLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVL--DFNN 120
            LN L L  N+F+ + IP+    L  + YL+L +    G +   +   + L+++  D+NN
Sbjct: 123 ELNQLILYLNYFSGS-IPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNN 182

Query: 121 KGQLNGPHNFPLGELF-----------IDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDV 180
              L G     LG+L            + G     I +L  +  +DLSG           
Sbjct: 183 ---LTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGN---------- 242

Query: 181 DYLIQLLNSIPSCFS--LNLSSCAL-QNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPI 240
               QL   IP  F   LNL S  L +N    D P      S L  L+L  N+  G IP 
Sbjct: 243 ----QLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPA 302

Query: 241 ILRNMTSLRYLN-----LNGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGE 300
            L N+  L+ L      L   + S +     +  L L  N+ V          I   +G 
Sbjct: 303 ELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGP--------ISEEIGF 362

Query: 301 LKSLKSLSLRGNALHGPIPSSFGNL--------------SRIPISFGQLRNLNTFDVSKN 360
           L+SL+ L+L  N   G  P S  NL                +P   G L NL       N
Sbjct: 363 LESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDN 422

Query: 361 SLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTW 420
            L G I  +  +N + LK++ +S N+                            G IP  
Sbjct: 423 LLTGPIPSS-ISNCTGLKLLDLSHNQMT--------------------------GEIPRG 482

Query: 421 LSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLG 480
               N  ++ I  N  +G +PD+I +   N+    +++N ++ G+L P + K + L  L 
Sbjct: 483 FGRMNLTFISIGRNHFTGEIPDDI-FNCSNLETLSVADN-NLTGTLKPLIGKLQKLRILQ 542

Query: 481 LSNNELSGTIPSCL-LIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQ 540
           +S N L+G IP  +  + +L + YL SN F+G  PR   +          L  LQ L + 
Sbjct: 543 VSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSN----------LTLLQGLRMY 602

Query: 541 GNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQY 600
            N  +  IP  ++ +  L +L L++NK  G+IP + +K  +                   
Sbjct: 603 SNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLES------------------- 662

Query: 601 VICYMSYLRQVMKSNNLNDSYVHVYSMVNV-DLSNNNLQGHIPREIVMINSLSN----LN 660
            + Y+S   Q  K N    + +   S++N  D+S+N L G IP E++   SL N    LN
Sbjct: 663 -LTYLSL--QGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLA--SLKNMQLYLN 690

Query: 661 LSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIP 662
            S+N L+G+IP E+G+   ++ +DLS+N  SGSIP +++   ++  L+ S NN SG IP
Sbjct: 723 FSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIP 690


HSP 3 Score: 152.9 bits (385), Expect = 1.4e-35
Identity = 184/660 (27.88%), Postives = 270/660 (40.91%), Query Frame = 1

Query: 67  LLGLTYLNLSSTYFHGAI----QPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDGLA 126
           LL  T+L L+ T+F   I    Q F   +  L       K   NG  N PLG L      
Sbjct: 3   LLSKTFLILTLTFFFFGIALAKQSFEPEIEAL-------KSFKNGISNDPLGVLS----D 62

Query: 127 FRWISSLRCVEYIDLSGVRVVQSGKL-DVDYLIQLLNSIPSCFSLNLSSCALQNYQLLDA 186
           +  I SLR   + + +G+    +G +  V  L + L  + S    NL+       Q+LD 
Sbjct: 63  WTIIGSLR---HCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYL-----QVLDL 122

Query: 187 PLNS---------SFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLNGGLSS--MVRN 246
             NS            ++L  L L  N F G IP  +  + ++ YL+L   L S  +   
Sbjct: 123 TSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEE 182

Query: 247 NCHMKSLDLL---FNNF---VEEMIGFGMH-------------TIPSWLGELKSLKSLSL 306
            C   SL L+   +NN    + E +G  +H             +IP  +G L +L  L L
Sbjct: 183 ICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDL 242

Query: 307 RGNALHGPIPSSFGNLSR--------------IPISFGQLRNLNTFDVSKNSLKGVITET 366
            GN L G IP  FGNL                IP   G   +L   ++  N L G I   
Sbjct: 243 SGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIP-A 302

Query: 367 HFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLSFPNFIYL 426
              NL QL+ + +  N+  S          S+ F    +TQ   LG              
Sbjct: 303 ELGNLVQLQALRIYKNKLTS-------SIPSSLFR---LTQLTHLG-------------- 362

Query: 427 DISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGT 486
            +S N + GP+ + IG+ + ++    + +N +  G  P S+   R+L  L +  N +SG 
Sbjct: 363 -LSENHLVGPISEEIGF-LESLEVLTLHSN-NFTGEFPQSITNLRNLTVLTVGFNNISGE 422

Query: 487 IPSCL-LIPNLTVFYLSSNKFSGVFP---------------RNQFSGSIPSWMGRRLQSL 546
           +P+ L L+ NL       N  +G  P                NQ +G IP   GR   +L
Sbjct: 423 LPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGR--MNL 482

Query: 547 QILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCN 606
             +++  N F   IP  ++    L+ L +ADN L G + P+  K     +K  +  V  N
Sbjct: 483 TFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKL----QKLRILQVSYN 542

Query: 607 SKEDQYVICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNL 662
           S             R++    +LN  Y+H          +N   G IPRE+  +  L  L
Sbjct: 543 SLTGPIP-------REIGNLKDLNILYLH----------SNGFTGRIPREMSNLTLLQGL 592

BLAST of Cucsa.054620 vs. Swiss-Prot
Match: Y1571_ARATH (Probable leucine-rich repeat receptor-like protein kinase At1g35710 OS=Arabidopsis thaliana GN=At1g35710 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 2.5e-45
Identity = 185/631 (29.32%), Postives = 272/631 (43.11%), Query Frame = 1

Query: 64  FAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDGLAF 123
           F  L  L Y++LS     G I P  GNL+KL+  D +    L G  +  LG L    + +
Sbjct: 98  FISLSNLAYVDLSMNLLSGTIPPQFGNLSKLIYFDLSTN-HLTGEISPSLGNLKNLTVLY 157

Query: 124 RWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCAL----QNYQLL 183
              + L  V   +L  +  +    L  +   +L  SIPS    NL +  +    +NY   
Sbjct: 158 LHQNYLTSVIPSELGNMESMTDLALSQN---KLTGSIPSSLG-NLKNLMVLYLYENYLTG 217

Query: 184 DAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNL-----NGGLSSMVRNNCHM 243
             P        +  L LS N+  G IP  L N+ +L  L L      G +   + N   M
Sbjct: 218 VIPPELGNMESMTDLALSQNKLTGSIPSTLGNLKNLMVLYLYENYLTGVIPPEIGNMESM 277

Query: 244 KSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNL------- 303
            +L L  N            +IPS LG LK+L  LSL  N L G IP   GN+       
Sbjct: 278 TNLALSQNKLTG--------SIPSSLGNLKNLTLLSLFQNYLTGGIPPKLGNIESMIDLE 337

Query: 304 -------SRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEI 363
                    IP S G L+NL    + +N L GVI      N+  +  + +++N+ L+  I
Sbjct: 338 LSNNKLTGSIPSSLGNLKNLTILYLYENYLTGVI-PPELGNMESMIDLQLNNNK-LTGSI 397

Query: 364 KHDWGFGSNGFHRWLVTQKDELGPIPTWL-SFPNFIYLDISNNQISGPLPDNIGYQIPNI 423
              +G   N  + +L       G IP  L +  + I LD+S N+++G +PD+ G     +
Sbjct: 398 PSSFGNLKNLTYLYLYLNY-LTGVIPQELGNMESMINLDLSQNKLTGSVPDSFG-NFTKL 457

Query: 424 FAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLL----IPNLTVFYLSSN 483
            + Y+  N H++G++PP V    HL  L L  N  +G  P  +     + N+++ Y    
Sbjct: 458 ESLYLRVN-HLSGAIPPGVANSSHLTTLILDTNNFTGFFPETVCKGRKLQNISLDY---- 517

Query: 484 KFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKL 543
                   N   G IP  + R  +SL      GN F   I  +  I P L  +  + NK 
Sbjct: 518 --------NHLEGPIPKSL-RDCKSLIRARFLGNKFTGDIFEAFGIYPDLNFIDFSHNKF 577

Query: 544 EGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDSYV----HV 603
            GEI       ++ +EKS   G +                  +M +NN+  +      ++
Sbjct: 578 HGEI-------SSNWEKSPKLGAL------------------IMSNNNITGAIPTEIWNM 637

Query: 604 YSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQ 663
             +V +DLS NNL G +P  I  + +LS L L+ N LSG +P  +     LESLDLSSN 
Sbjct: 638 TQLVELDLSTNNLFGELPEAIGNLTNLSRLRLNGNQLSGRVPAGLSFLTNLESLDLSSNN 672


HSP 2 Score: 176.0 bits (445), Expect = 1.5e-42
Identity = 205/735 (27.89%), Postives = 302/735 (41.09%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEYFYSSAFLSNGVDSSLFESKYLNYLGLSANFFNYTPI 60
           W GV C+  + G + +L+L N      +       D        L Y+ LS N  + T I
Sbjct: 68  WYGVSCN--SRGSIEELNLTN------TGIEGTFQDFPFISLSNLAYVDLSMNLLSGT-I 127

Query: 61  PNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNK----------GQLNGPHN 120
           P  F  L  L Y +LS+ +  G I P LGNL  L VL  +            G +    +
Sbjct: 128 PPQFGNLSKLIYFDLSTNHLTGEISPSLGNLKNLTVLYLHQNYLTSVIPSELGNMESMTD 187

Query: 121 FPLGELFIDGLAFRWISSLR--CVEYID---LSGVRVVQSGKLD--VDYLI---QLLNSI 180
             L +  + G     + +L+   V Y+    L+GV   + G ++   D  +   +L  SI
Sbjct: 188 LALSQNKLTGSIPSSLGNLKNLMVLYLYENYLTGVIPPELGNMESMTDLALSQNKLTGSI 247

Query: 181 PSCFSLNLSSCAL----QNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLR 240
           PS    NL +  +    +NY     P        + +L LS N+  G IP  L N+ +L 
Sbjct: 248 PSTLG-NLKNLMVLYLYENYLTGVIPPEIGNMESMTNLALSQNKLTGSIPSSLGNLKNLT 307

Query: 241 YLNL-----NGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSL 300
            L+L      GG+   + N   M  L+L  N            +IPS LG LK+L  L L
Sbjct: 308 LLSLFQNYLTGGIPPKLGNIESMIDLELSNNKLTG--------SIPSSLGNLKNLTILYL 367

Query: 301 RGNALHGPIPSSFGNL--------------SRIPISFGQLRNLNTFDVSKNSLKGVITET 360
             N L G IP   GN+                IP SFG L+NL    +  N L GVI + 
Sbjct: 368 YENYLTGVIPPELGNMESMIDLQLNNNKLTGSIPSSFGNLKNLTYLYLYLNYLTGVIPQ- 427

Query: 361 HFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLSFPNFIYL 420
              N+  +  + +S N+                            G +P   SF NF  L
Sbjct: 428 ELGNMESMINLDLSQNK--------------------------LTGSVPD--SFGNFTKL 487

Query: 421 D---ISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNEL 480
           +   +  N +SG +P  +            +NN    G  P +VCK R L  + L  N L
Sbjct: 488 ESLYLRVNHLSGAIPPGVANSSHLTTLILDTNN--FTGFFPETVCKGRKLQNISLDYNHL 547

Query: 481 SGTIPSCLL-IPNLTVFYLSSNKFSG---------------VFPRNQFSGSIPS-WMGRR 540
            G IP  L    +L       NKF+G                F  N+F G I S W   +
Sbjct: 548 EGPIPKSLRDCKSLIRARFLGNKFTGDIFEAFGIYPDLNFIDFSHNKFHGEISSNW--EK 607

Query: 541 LQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSG 600
              L  L +  N+    IP  +W + +L  L L+ N L GE+P       T   +  ++G
Sbjct: 608 SPKLGALIMSNNNITGAIPTEIWNMTQLVELDLSTNNLFGELPEAIGNL-TNLSRLRLNG 667

Query: 601 VMCNSKEDQYVICYMSYLRQV----MKSNNLN-------DSYVHVYSMVNVDLSNNNLQG 660
              + +    V   +S+L  +    + SNN +       DS++ ++ M   +LS N   G
Sbjct: 668 NQLSGR----VPAGLSFLTNLESLDLSSNNFSSEIPQTFDSFLKLHDM---NLSRNKFDG 727

Query: 661 HIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSL 662
            IPR +  +  L+ L+LSHN L G IP ++     L+ LDLS N LSG IP   E + +L
Sbjct: 728 SIPR-LSKLTQLTQLDLSHNQLDGEIPSQLSSLQSLDKLDLSHNNLSGLIPTTFEGMIAL 742


HSP 3 Score: 131.0 bits (328), Expect = 5.7e-29
Identity = 118/417 (28.30%), Postives = 194/417 (46.52%), Query Frame = 1

Query: 319 FANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLSFP-----N 378
           F N S+L      +N + SF     +G   N             G   T+  FP     N
Sbjct: 44  FTNSSKLSSWVHDANTNTSFSCTSWYGVSCNSRGSIEELNLTNTGIEGTFQDFPFISLSN 103

Query: 379 FIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNE 438
             Y+D+S N +SG +P   G  +  +  F +S N H+ G + PS+   ++L  L L  N 
Sbjct: 104 LAYVDLSMNLLSGTIPPQFG-NLSKLIYFDLSTN-HLTGEISPSLGNLKNLTVLYLHQNY 163

Query: 439 LSGTIPSCL-LIPNLTVFYLSSNKFSGVFP---------------RNQFSGSIPSWMGRR 498
           L+  IPS L  + ++T   LS NK +G  P                N  +G IP  +G  
Sbjct: 164 LTSVIPSELGNMESMTDLALSQNKLTGSIPSSLGNLKNLMVLYLYENYLTGVIPPELGN- 223

Query: 499 LQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPP----IEAKFATKFEKS 558
           ++S+  L L  N    +IP +L  L  L +L L +N L G IPP    +E+       ++
Sbjct: 224 MESMTDLALSQNKLTGSIPSTLGNLKNLMVLYLYENYLTGVIPPEIGNMESMTNLALSQN 283

Query: 559 TVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIV 618
            ++G + +S  +   +  +S  +  + +  +     ++ SM++++LSNN L G IP  + 
Sbjct: 284 KLTGSIPSSLGNLKNLTLLSLFQNYL-TGGIPPKLGNIESMIDLELSNNKLTGSIPSSLG 343

Query: 619 MINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSN 678
            + +L+ L L  N L+G IP E+G    +  L L++N+L+GSIP +   L +L  L L  
Sbjct: 344 NLKNLTILYLYENYLTGVIPPELGNMESMIDLQLNNNKLTGSIPSSFGNLKNLTYLYLYL 403

Query: 679 NNFSGRIPR--GG-----NPHLCGDPFPNTCPNENGNHSDVKDKLETMWFCVIVVAG 704
           N  +G IP+  G      N  L  +    + P+  GN +    KLE+++  V  ++G
Sbjct: 404 NYLTGVIPQELGNMESMINLDLSQNKLTGSVPDSFGNFT----KLESLYLRVNHLSG 452

BLAST of Cucsa.054620 vs. TrEMBL
Match: M5WZX0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001022mg PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 5.5e-124
Identity = 327/893 (36.62%), Postives = 446/893 (49.94%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEYFYSSAFLSNGVD-SSLFESKYLNYLGLSANFFNYTP 60
           WKGV CD  + GHVVKLDLR         F +  V  S L E KYL YL LS N     P
Sbjct: 64  WKGVSCDDQSAGHVVKLDLRAKRLRTEVHFQATEVSLSCLLELKYLKYLDLSGNNLGDGP 123

Query: 61  IPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFID 120
           IP  F  +  LTYLNLSST F G +   LGNLT L +LD +N+         PL  L +D
Sbjct: 124 IPKFFGSMKQLTYLNLSSTQFSGTVPHHLGNLTSLQILDLSNQ---------PL--LIVD 183

Query: 121 GLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLL 180
            L   W+S L  + ++D+S + + ++  L     +Q+L+ +PS   L LS+C L   Q  
Sbjct: 184 DLL--WLSRLSSLHHLDMSDLYLGETLNL-----MQVLSMLPSLLWLRLSNCGLDETQFP 243

Query: 181 DAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLR----------------------- 240
               N++F   LQ LDL+ N   GPIP  LRNMTSLR                       
Sbjct: 244 GGGTNATFLHNLQSLDLANNGLLGPIPDALRNMTSLRRLDLSFNILQSSIPAWLGNLENL 303

Query: 241 -YLN--------LNGGLSSMVRNNCHMKSLDLLFNNFVEEMIG----------FGMHT-- 300
            +LN        + GGL S++RN CH+KSLDL  N F  E++G          + + T  
Sbjct: 304 VHLNLSHNIFNSIEGGLLSILRNACHLKSLDLSLNQFQGEILGTSGTLSGCTEYDLDTLR 363

Query: 301 ---------IPSWLGELKSLKSLSLRGNALHGPIPSSFGNLS--------------RIPI 360
                    +P WLG+L++LK L  + N+  GPIP S GNLS               +P 
Sbjct: 364 LVNNGASGRLPDWLGKLRNLKYLDFQMNSFSGPIPFSLGNLSTLRELYLSHNQLNGTVPE 423

Query: 361 SFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDW-------- 420
           S GQL NL   D+S N++ G+++E H ANLS+L+++  + N HL+  +K +W        
Sbjct: 424 SLGQLSNLTVLDLSSNNIDGIVSEVHIANLSKLEILNFALN-HLTIRVKSNWEPPFQLKH 483

Query: 421 ------GFGSNGFHRWLVTQK---------DELGPIPTWLSFPNFIYLDISNNQISGPLP 480
                  FG+  F +WL+TQK            G IP  L   +  YLD+S N+I+G LP
Sbjct: 484 VRMESCKFGTQ-FPQWLLTQKTITLDLSNASISGTIPKSLHGLHLTYLDLSYNEITGSLP 543

Query: 481 DNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLL-IPNLT 540
                 +P + + ++ +N HI GS+P S+C       L LS N LSG IP CL  + NL 
Sbjct: 544 QKFSDGMPRLDSLFLGSN-HIKGSVPTSLCNIETSGALDLSKNRLSGEIPGCLKNLQNLY 603

Query: 541 VFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQIL 600
              LSSN  SGV         IPS +G  L+ L  LNL  NS    +P +L     L+ L
Sbjct: 604 AVDLSSNNLSGV---------IPSSVG-YLRHLVWLNLNNNSLHGELPSALRNCSYLKHL 663

Query: 601 ILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQY--VICYMSYLRQVMKSNNLND 660
            L +N+L G IP   A     FE   +  +  N+        +C +S L+ V  S N  +
Sbjct: 664 DLGNNRLSGHIP---AWIGDSFEFLIILRLRSNTFNGSIPSQLCQLSPLQLVQYSTNQPE 723

Query: 661 SYVHVYSM---------------------------VNVDLSNNNLQGHIPREIVMINSLS 720
           S    Y +                           V++DLS+NNL G IP E+  ++ L 
Sbjct: 724 SDQQGYDIDAEPNNKNIWQVLKGREYESARISSLGVSMDLSSNNLVGFIPDELTSLSGLL 783

Query: 721 NLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKL--NSLRALNLSNNNFS 745
            LNLSHN+L GSIP +IG    LES D+S+NQLSG+IP++M  L   +L  LNLS NN S
Sbjct: 784 GLNLSHNHLEGSIPQKIGELKSLESFDISNNQLSGTIPMSMWSLAMATLSHLNLSYNNLS 843

BLAST of Cucsa.054620 vs. TrEMBL
Match: M5WZX0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001022mg PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.2e-15
Identity = 137/526 (26.05%), Postives = 217/526 (41.25%), Query Frame = 1

Query: 153 LIQLLNSIPSCFSLNLSSCALQNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNM 212
           L+  L +IP CF +N ++  ++     D      F+   Q     ++ + G      ++ 
Sbjct: 12  LVLSLAAIPFCFCINSTASCVER----DREALIKFQQSFQDTPDGFSSWKG------KDC 71

Query: 213 TSLRYLNLNGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLR 272
              +      G+S   ++  H+  LDL       E+         S L ELK LK L L 
Sbjct: 72  CKWK------GVSCDDQSAGHVVKLDLRAKRLRTEVHFQATEVSLSCLLELKYLKYLDLS 131

Query: 273 GNAL-HGPIPSSFGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVS 332
           GN L  GPIP  FG++        QL  LN   +S     G +   H  NL+ L+++ +S
Sbjct: 132 GNNLGDGPIPKFFGSMK-------QLTYLN---LSSTQFSGTVP-HHLGNLTSLQILDLS 191

Query: 333 SNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLS-FPNFIYLDISNNQISGPLP- 392
           +   L                       D+L     WLS   +  +LD+S+  +   L  
Sbjct: 192 NQPLLIV---------------------DDL----LWLSRLSSLHHLDMSDLYLGETLNL 251

Query: 393 DNIGYQIPNIFAFYISNNMHINGSLP---PSVCKWRHLVFLGLSNNELSGTIPSCLLIPN 452
             +   +P++    +SN        P    +     +L  L L+NN L G IP  L   N
Sbjct: 252 MQVLSMLPSLLWLRLSNCGLDETQFPGGGTNATFLHNLQSLDLANNGLLGPIPDALR--N 311

Query: 453 LTVFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPR-- 512
           +T        F      N    SIP+W+G  L++L  LNL  N F+      L IL    
Sbjct: 312 MTSLRRLDLSF------NILQSSIPAWLGN-LENLVHLNLSHNIFNSIEGGLLSILRNAC 371

Query: 513 -LQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMK--S 572
            L+ L L+ N+ +GEI              T+SG  C   +       +  LR V    S
Sbjct: 372 HLKSLDLSLNQFQGEILGTSG---------TLSG--CTEYD-------LDTLRLVNNGAS 431

Query: 573 NNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLR 632
             L D    + ++  +D   N+  G IP  +  +++L  L LSHN L+G++P  +G+   
Sbjct: 432 GRLPDWLGKLRNLKYLDFQMNSFSGPIPFSLGNLSTLRELYLSHNQLNGTVPESLGQLSN 458

Query: 633 LESLDLSSNQLSGSI-PLNMEKLNSLRALNLSNNNFSGRIPRGGNP 667
           L  LDLSSN + G +  +++  L+ L  LN + N+ + R+     P
Sbjct: 492 LTVLDLSSNNIDGIVSEVHIANLSKLEILNFALNHLTIRVKSNWEP 458


HSP 2 Score: 392.9 bits (1008), Expect = 8.9e-106
Identity = 207/260 (79.62%), Postives = 210/260 (80.77%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEYFYSSAFLSNGVDSSLFESKYLNYLGLSANFFNYTPI 60
           WKGVGCDHTNGGHVVKLDLRNYEYFYSSA LSNGVDSSLFESKYLNYLGLSANFFNYTPI
Sbjct: 29  WKGVGCDHTNGGHVVKLDLRNYEYFYSSALLSNGVDSSLFESKYLNYLGLSANFFNYTPI 88

Query: 61  PNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDG 120
           PNSF GLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLN              
Sbjct: 89  PNSFGGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLN-------------- 148

Query: 121 LAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLLD 180
                       EY+DLSGVRVV+SGKLDVDYLIQLLNSIPSCFSLNLSS ALQNYQLLD
Sbjct: 149 ------------EYLDLSGVRVVESGKLDVDYLIQLLNSIPSCFSLNLSSSALQNYQLLD 208

Query: 181 APLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLNGGLSSMVRNNCHMKSLDLL 240
           APLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLNG         C    L  L
Sbjct: 209 APLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLNG---------CKEYGLQRL 250

Query: 241 FNNFVEEMIGFGMHTIPSWL 261
              + EEMIGFGMHTIP  L
Sbjct: 269 ---YPEEMIGFGMHTIPKAL 250

BLAST of Cucsa.054620 vs. TrEMBL
Match: A0A059BIT6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G03161 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 9.2e-103
Identity = 297/866 (34.30%), Postives = 420/866 (48.50%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDL-------RNYEYFYSSAFL---SNGVDSSLFESKYLNYLGL 60
           W+GV CD   G HV+ L L           YF     L   S  ++SSL   KYLN+L L
Sbjct: 77  WEGVICDRVYG-HVISLQLLPQPTKDETSTYFRMGPKLLQASGELNSSLLHLKYLNHLDL 136

Query: 61  SANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHN 120
           S    N+  IP     +  L YLNL    FHG +   LGNLTKL VLD  N+ +      
Sbjct: 137 SGIDSNHGRIPEFIGSIKQLRYLNLLFANFHGTVPQQLGNLTKLEVLDLGNEDE------ 196

Query: 121 FPLGELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSS 180
                L +D +   W+S LR ++Y+D+SG+ + +         +Q+ + +P+   L+LS 
Sbjct: 197 ----NLVVDDIL--WVSHLRSLKYLDMSGLMIAKER-----VPMQVTSMLPALLHLSLSG 256

Query: 181 CALQNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRN-MTSLRYL--NLNGGLSSM 240
           C L N+ +     N    S   HLDLS+N F+  IP+   N + SL     +L G L + 
Sbjct: 257 CGLHNFHMSS---NHFANSTSLHLDLSWNSFNSSIPMWFYNSLESLEICENHLKGALPNW 316

Query: 241 VRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNL 300
           + +  ++KS+ L  N F           IPS +G L +L++L L  N L+G IPSS G L
Sbjct: 317 LEHFTNLKSIMLSSNFF--------SCPIPSVIGSLSNLETLDLSDNGLNGTIPSSIGKL 376

Query: 301 S--------------RIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSN 360
           S               IP S G+L  L    +  N L+GVITE HF+NLS+LK  +++ N
Sbjct: 377 SSLRSLDLSYNELVGHIPKSLGKLGALQELLLYNNHLEGVITEIHFSNLSRLKEYSINGN 436

Query: 361 EHLSFEIKHDW---------GFGSNGFH----RWLVTQKDE-----LGPIPTWLSFPNFI 420
            +LSF+ K  W         G  S  F     RW+ TQ D       GP+P   +  N  
Sbjct: 437 NNLSFKAKPSWIPPFQLEIIGMSSCKFEVEVPRWIQTQLDLSYNQITGPLPNMST--NCS 496

Query: 421 YLDISNNQISGPLPDNI----------------GYQIPN------IFAFYISNNMHINGS 480
           +LD+S+N ISG L  NI                   +P+      +    ++NN  ++GS
Sbjct: 497 FLDLSHNLISGSLLTNISTMYQVRILYLNDNLIDGTLPSSLCNLELIVLNLANNS-LSGS 556

Query: 481 LPPSVCKWRHLVFLGLSNNELSGTIPSCL-LIPNLTVFYLSSNKFSGVFPR--------- 540
           +    C    L  L LS N+LSG IPS L  +P+LT  +L+ N+ +G  P+         
Sbjct: 557 ILD--CWKDSLSLLTLSFNKLSGVIPSSLGSLPHLTTLHLNRNRLNGELPQALQYCTSLV 616

Query: 541 ------NQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEG 600
                 N  SGSIP+W      SL IL L+ N F  +IP  L  L RL+IL +A N L G
Sbjct: 617 IIDLGENNLSGSIPTWFDGTFHSLLILRLRENRFVGSIPPQLCSLSRLRILDMAMNNLTG 676

Query: 601 EIPPIEAKFATKFE----KSTVSGVMCNSKE-----DQYVICYMSYLRQVMKSNNLND-S 660
            IP      ++        S  S  + +S        +Y    ++ +  ++     N+ +
Sbjct: 677 MIPHCLGNMSSMINLNQTNSFGSVAIAHSSSLSLLMGRYYSPVLTLVTLLLLKGRYNEYT 736

Query: 661 YVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDL 720
            + +  +VN+DLS+N L G IP E+  ++ L  LNLSHN+LSG+IP+ IG    LESLDL
Sbjct: 737 KIVLQLVVNLDLSSNFLNGSIPEELSFLSRLHGLNLSHNHLSGNIPIGIGNMTSLESLDL 796

Query: 721 SSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIPRG-------------GNPHLCGD--- 750
           S+N LSG+IP  +  L SL  LNLS N+  G+IP+G             GNP LCGD   
Sbjct: 797 SNNHLSGTIPQGISALTSLAHLNLSQNDLMGQIPKGNQIQTLDDPSIYAGNPLLCGDLLR 856

BLAST of Cucsa.054620 vs. TrEMBL
Match: A0A0A0KL22_CUCSA (Serine/threonine-protein kinase bri1 OS=Cucumis sativus GN=Csa_5G167740 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 2.0e-97
Identity = 252/608 (41.45%), Postives = 340/608 (55.92%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLR-NYEYFYSSAFLS-NGVDSSLFESKYLNYLGLSANFFNYT 60
           W GVGC+ T G +V  +DLR +Y    S   LS N +DSSL E KYLN+L LS N+FN+T
Sbjct: 67  WNGVGCNQTTG-YVTIIDLRQDYNQVDSYESLSSNFIDSSLLELKYLNHLDLSGNYFNHT 126

Query: 61  PIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFN-NKGQLNGPHNFPL-GEL 120
            IPN    +L LTYLNLS T   G I P LGNL+KL +LD + +  + +     PL G++
Sbjct: 127 QIPNFLGSMLELTYLNLSRTSLIGKIPPHLGNLSKLEILDLDRDYYEFDATRWVPLNGDI 186

Query: 121 FIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNY 180
                   WIS L  +  + LSG+    S  ++   L+Q+++S+PS  SL L SC+LQN 
Sbjct: 187 -------EWISHLSSLHTLHLSGMNF--SNAIN---LMQVISSLPSLSSLRLRSCSLQNN 246

Query: 181 QL-LDAPLN-SSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLN---------GGLS 240
              L + LN SSF S++Q LDL  N+  G IP I +NMTSL+YL+L+         GG+S
Sbjct: 247 DFSLGSWLNYSSFISRVQLLDLHDNQLSGQIPTIFQNMTSLKYLDLSLNNFTAIFEGGIS 306

Query: 241 SMVRNNCHMKSLDLLFN-----------NFVEEMIGFGMHT-----------IPSWLGEL 300
           + + NNC +K LDL  N           ++  E +G  +             IP+WL +L
Sbjct: 307 TFIENNCGLKVLDLSLNFYFGTDDVFESSYENETMGCDLQVLNLRYTSLKTKIPNWLEKL 366

Query: 301 KSLKSLSLRGNALHGPIPSSFGNLSR--------------IPISFGQLRNLNTFDVSKNS 360
           K+L+++ L+ + ++G IP+S GNLS               IP SF +L NL   DVS+NS
Sbjct: 367 KNLQTIDLQSSQIYGLIPTSLGNLSNLKYLDLSINNLTGTIPASFERLLNLEVLDVSENS 426

Query: 361 LKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWG---------------FGSNGFHRW 420
           LKGV+TE  FANL +L  + +  NE+LS EIK +W                FGS  F +W
Sbjct: 427 LKGVLTEASFANLYRLHTLNLGYNENLSLEIKANWNPPFQLLFFDASRICCFGSE-FPQW 486

Query: 421 LVTQKDELG-----------PIPTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFY 480
           L TQK  +G            IPTW +  N I L++SNNQI GPLP NIG Q+P +    
Sbjct: 487 LQTQKALVGLSLSNTNLSISSIPTWFTPQNLITLNLSNNQIMGPLPTNIGDQMPKLQRLR 546

Query: 481 ISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLLIPNLTVFYLSSNKFSGVFP- 514
           +++N+ I+GSLP S+C+W++L  L LSNN LSG I  C L PNL +  LSSN FSG+FP 
Sbjct: 547 LNDNL-IDGSLPRSICRWKNLNVLNLSNNRLSGMIEGCFLNPNLYLLDLSSNNFSGIFPY 606

BLAST of Cucsa.054620 vs. TrEMBL
Match: A0A0A0KL22_CUCSA (Serine/threonine-protein kinase bri1 OS=Cucumis sativus GN=Csa_5G167740 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.2e-57
Identity = 220/662 (33.23%), Postives = 315/662 (47.58%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLR-NYEYFYSSAFLS-NGVDSSLFESKYLNYLGLSANFFNYT 60
           W GVGC+ T G +V  +DLR +Y    S   LS N +DSSL E KYLN+L LS N+FN+T
Sbjct: 67  WNGVGCNQTTG-YVTIIDLRQDYNQVDSYESLSSNFIDSSLLELKYLNHLDLSGNYFNHT 126

Query: 61  PIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKG-QLNGPHNFPL-GEL 120
            IPN    +L LTYLNLS T   G I P LGNL+KL +LD +    + +     PL G++
Sbjct: 127 QIPNFLGSMLELTYLNLSRTSLIGKIPPHLGNLSKLEILDLDRDYYEFDATRWVPLNGDI 186

Query: 121 FIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNY 180
                   WIS L  +  + LSG+    +  L     +Q+++S+PS  SL L SC+LQN 
Sbjct: 187 -------EWISHLSSLHTLHLSGMNFSNAINL-----MQVISSLPSLSSLRLRSCSLQNN 246

Query: 181 QL-LDAPLN-SSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLN---------GGLS 240
              L + LN SSF S++Q LDL  N+  G IP I +NMTSL+YL+L+         GG+S
Sbjct: 247 DFSLGSWLNYSSFISRVQLLDLHDNQLSGQIPTIFQNMTSLKYLDLSLNNFTAIFEGGIS 306

Query: 241 SMVRNNCHMKSLDLLFNNFVEEMIGFGMHTI--PSWLGELKS--LKSLSLRGNALHGPIP 300
           + + NNC +K LDL  N +      FG   +   S+  E     L+ L+LR  +L     
Sbjct: 307 TFIENNCGLKVLDLSLNFY------FGTDDVFESSYENETMGCDLQVLNLRYTSLK---- 366

Query: 301 SSFGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIK 360
                 ++IP    +L+NL T D+  + + G+I  T   NLS LK        +L   I 
Sbjct: 367 ------TKIPNWLEKLKNLQTIDLQSSQIYGLIP-TSLGNLSNLK--------YLDLSIN 426

Query: 361 HDWGFGSNGFHRWLVTQ-----KDELGPIPTWLSFPNFIYLDISNNQISGPLPDNI--GY 420
           +  G     F R L  +     ++ L  + T  SF N   L   N   +  L   I   +
Sbjct: 427 NLTGTIPASFERLLNLEVLDVSENSLKGVLTEASFANLYRLHTLNLGYNENLSLEIKANW 486

Query: 421 QIPNIFAFYISNNMHINGS-LPPSVCKWRHLVFLGLSNNELS-GTIPSCLLIPNLTVFYL 480
             P    F+ ++ +   GS  P  +   + LV L LSN  LS  +IP+     NL    L
Sbjct: 487 NPPFQLLFFDASRICCFGSEFPQWLQTQKALVGLSLSNTNLSISSIPTWFTPQNLITLNL 546

Query: 481 SSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILAD 540
           S+N+  G  P N         +G ++  LQ L L  N  D ++P S+     L +L L++
Sbjct: 547 SNNQIMGPLPTN---------IGDQMPKLQRLRLNDNLIDGSLPRSICRWKNLNVLNLSN 606

Query: 541 NKLEGEIPPIEAK---FATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDSYV 600
           N+L G I         +      +  SG+   S E+      +SY+ QV           
Sbjct: 607 NRLSGMIEGCFLNPNLYLLDLSSNNFSGIFPYSHEN------LSYIEQVY---------- 656

Query: 601 HVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLR-LESLDLS 631
                    L NNN +G +P  +    SL  L+L+ N  SG+IP  +G +L  L+ L L 
Sbjct: 667 ---------LRNNNFEGSMPIVLKNAKSLEILDLTGNKFSGTIPTWVGENLESLQFLILR 656


HSP 2 Score: 341.3 bits (874), Expect = 3.1e-90
Identity = 266/769 (34.59%), Postives = 377/769 (49.02%), Query Frame = 1

Query: 70  LTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDGLAFRWISSL 129
           L  L+LS ++F G I   LGNLTKLL+LDF+    L           ++D   + WIS L
Sbjct: 9   LQVLSLSDSHFSGRIPYHLGNLTKLLLLDFSFNSLL-----------YVDD--YNWISQL 68

Query: 130 RCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQL----------- 189
             ++Y+ +S V +   GK     L+Q LN++PS   + L +C L                
Sbjct: 69  SSLQYLYMSDVHL---GK--AQNLLQALNTLPSLLEIELMNCTLNKLHTHQHVKATNLSR 128

Query: 190 ----------LDAPLNSSFRS--KLQHLDLSYNEFDGPIPIILRNMTSLRYLNLNGG--- 249
                     L AP   +F++   L  +DLSYN     IP  LRN  +L  L +N     
Sbjct: 129 LEVLNLAENGLQAPFLDAFQNMTSLAEIDLSYNNLSS-IPFWLRNCINLLNLYVNSNALY 188

Query: 250 --LSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIP 309
             L S ++N   + SLDL  NNF           +PSW  +L  L  L+L  N+L+G IP
Sbjct: 189 ASLPSALQNLTSLISLDLSENNF---------DYVPSWY-DLMQL-DLNLTRNSLNGTIP 248

Query: 310 SSFGNLSR--------------IPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKM 369
            + G+L                IP SFGQL NL   D+S N L     E+  ++++  K 
Sbjct: 249 QNIGHLKNLITLYLSDNILHGNIPRSFGQLSNLQNLDMSLNHL-----ESLVSDITWPKQ 308

Query: 370 VAVSS--NEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLSFPNFIYLDISNNQIS 429
           +   +  N H++  +  D                D L         PN  +L + NN IS
Sbjct: 309 IVYLNLTNNHITGSLPQDIA--------------DRL---------PNVTHLLLGNNLIS 368

Query: 430 GPLPDNIGYQIPNIFAFYISNNMHINGSLPPS-VCKWRHLVFLGLSNNELSGTIPSCL-L 489
           G +P ++  +I +++   +S N+ ++G +P       ++L  + L++N+LSG IPS L  
Sbjct: 369 GSIPKSL-CKIDSLYNLDLSGNL-LSGEIPNCWSATQQNLSEINLASNKLSGVIPSSLGN 428

Query: 490 IPNLTVFYLSSNKFSGVFP---------------RNQFSGSIPSWMGRRLQSLQILNLQG 549
           +  L   +L++N   G FP                N    +IP WMG    SLQIL L+ 
Sbjct: 429 LSTLAWLHLNNNSLHGGFPSSLRNLKHLLILDVGENHLFWTIPLWMGNIFSSLQILRLRQ 488

Query: 550 NSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYV 609
           N    TIP  L  L  L+IL L+DN L G IP     F         S     S+  +YV
Sbjct: 489 NKLHGTIPSQLCQLSALKILDLSDNNLMGSIPQCIGNFTGMISGKKSSSTQ-PSEGPRYV 548

Query: 610 ICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNL 669
             Y   +RQV+K   L D   ++  +VN+DLSNNNL G IP  I ++++L NLNLS+N+L
Sbjct: 549 EWYEQEVRQVIKGVEL-DYTRNLKLVVNMDLSNNNLSGSIPEGITLLSALHNLNLSYNHL 608

Query: 670 SGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIPRG----- 729
           SG IP  IG    LESLD+S +QLSG IP NM  L SL  LNLS NN SG IP G     
Sbjct: 609 SGRIPERIGDMKSLESLDISHDQLSGRIPDNMSSLTSLSHLNLSYNNLSGPIPEGRQLST 668

Query: 730 --------GNPHLCGDPFPNTCPNENGNHSDVKD----------KLETMWFCVIVVAGYS 755
                   GNP LCG P PN C  ++ +  D +D          KLE +WF  +V  GY+
Sbjct: 669 LDNPFIYAGNPFLCGPPRPNKCFTDDEDDDDDEDEDEDEDGKKGKLEKLWFYFVVAFGYA 715

BLAST of Cucsa.054620 vs. TAIR10
Match: AT2G34930.1 (AT2G34930.1 disease resistance family protein / LRR family protein)

HSP 1 Score: 213.4 bits (542), Expect = 4.9e-55
Identity = 206/723 (28.49%), Postives = 306/723 (42.32%), Query Frame = 1

Query: 37  SSLFESKYLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLV 96
           SS  + K L  L LS N  N +PIPN   GL  L  L L   +  G+I     NL  L  
Sbjct: 241 SSSADLKLLEVLDLSENSLN-SPIPNWLFGLTNLRKLFLRWDFLQGSIPTGFKNLKLLET 300

Query: 97  LDFNNKGQLNGPHNFPLGELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQL 156
           LD +N   L G     LG+L               ++++DLS   +  +G++        
Sbjct: 301 LDLSNNLALQGEIPSVLGDL-------------PQLKFLDLSANEL--NGQIHGFLDAFS 360

Query: 157 LNSIPSCFSLNLSSCALQNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLR 216
            N   S   L+LSS  L        P +      LQ LDLS N F G +P  + NM SL+
Sbjct: 361 RNKGNSLVFLDLSSNKLAG----TLPESLGSLRNLQTLDLSSNSFTGSVPSSIGNMASLK 420

Query: 217 YLNL-----NGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSL 276
            L+L     NG ++  +     +  L+L+ N +       G     S    L+SLKS+ L
Sbjct: 421 KLDLSNNAMNGTIAESLGQLAELVDLNLMANTW-------GGVLQKSHFVNLRSLKSIRL 480

Query: 277 RGN---ALHGPIPSSF-------------GNLSRIPISFGQLRNLNTFDVSKNSLKGVIT 336
                 +L   +PS++               +   P+       LN   +    ++  I 
Sbjct: 481 TTEPYRSLVFKLPSTWIPPFRLELIQIENCRIGLFPMWLQVQTKLNFVTLRNTGIEDTIP 540

Query: 337 ETHFANLSQLKMVAVSSNEHLSFEIKHDWGF--------GSNGFHRWLVTQKDELGPIPT 396
           ++ F+ +S      + +N  +   +     F         SN F           G  P 
Sbjct: 541 DSWFSGISSKVTYLILANNRIKGRLPQKLAFPKLNTIDLSSNNFE----------GTFPL 600

Query: 397 WLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFL 456
           W +  N   L +  N  SG LP NI   +P +   Y+ +N    G++P S+C+   L  L
Sbjct: 601 WST--NATELRLYENNFSGSLPQNIDVLMPRMEKIYLFSNS-FTGNIPSSLCEVSGLQIL 660

Query: 457 GLSNNELSGTIPSCL-------------------------LIPNLTVFYLSSNKFSGVFP 516
            L  N  SG+ P C                          ++P+L+V  L+ N   G  P
Sbjct: 661 SLRKNHFSGSFPKCWHRQFMLWGIDVSENNLSGEIPESLGMLPSLSVLLLNQNSLEGKIP 720

Query: 517 R---------------NQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQI 576
                           N+ +G +PSW+G+ L SL +L LQ NSF   IP  L  +P L+I
Sbjct: 721 ESLRNCSGLTNIDLGGNKLTGKLPSWVGK-LSSLFMLRLQSNSFTGQIPDDLCNVPNLRI 780

Query: 577 LILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDS 636
           L L+ NK+ G IP   +   T   + T      N++  Q ++  ++  R+     N    
Sbjct: 781 LDLSGNKISGPIPKCISNL-TAIARGT------NNEVFQNLVFIVTRAREYEAIAN---- 840

Query: 637 YVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDL 681
                   +++LS NN+ G IPREI+ +  L  LNLS N+++GSIP +I    RLE+LDL
Sbjct: 841 --------SINLSGNNISGEIPREILGLLYLRILNLSRNSMAGSIPEKISELSRLETLDL 900


HSP 2 Score: 174.1 bits (440), Expect = 3.3e-43
Identity = 197/709 (27.79%), Postives = 302/709 (42.60%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEYF-----YSSAFLSNGVDSSLFESKYLNYLGLSANFF 60
           W GV CD     HVVK+DLRN         Y    L   +  SL + K+L+YL LS+N F
Sbjct: 66  WPGVLCD-ARTSHVVKIDLRNPSQDVRSDEYKRGSLRGKIHPSLTQLKFLSYLDLSSNDF 125

Query: 61  NYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGE 120
           N   IP     ++ L YLNLSS+ F G I   LGNL+KL  LD   +   +       G 
Sbjct: 126 NELEIPEFIGQIVSLRYLNLSSSSFSGEIPTSLGNLSKLESLDLYAESFGDS------GT 185

Query: 121 LFIDGLAFRWISSLRC-VEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQ 180
           L +     RW+SSL   ++Y+++  V +  +G+      +Q  + I +   L+L +  L+
Sbjct: 186 LSLRASNLRWLSSLSSSLKYLNMGYVNLSGAGET----WLQDFSRISALKELHLFNSELK 245

Query: 181 NYQLLDAPLNSSFRSKL-QHLDLSYNEFDGPIPIILRNMTSLRYLNLN-----GGLSSMV 240
           N   L   L+SS   KL + LDLS N  + PIP  L  +T+LR L L      G + +  
Sbjct: 246 N---LPPTLSSSADLKLLEVLDLSENSLNSPIPNWLFGLTNLRKLFLRWDFLQGSIPTGF 305

Query: 241 RNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLS 300
           +N   +++LDL  N  ++         IPS LG+L  LK L L  N L+G I       S
Sbjct: 306 KNLKLLETLDLSNNLALQG-------EIPSVLGDLPQLKFLDLSANELNGQIHGFLDAFS 365

Query: 301 R-------------------IPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVA 360
           R                   +P S G LRNL T D+S NS  G +  +   N++ LK + 
Sbjct: 366 RNKGNSLVFLDLSSNKLAGTLPESLGSLRNLQTLDLSSNSFTGSVPSS-IGNMASLKKLD 425

Query: 361 VSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIP----TW---LSFPNFIYL-DISN 420
           +S+N               NG     + Q  EL  +     TW   L   +F+ L  + +
Sbjct: 426 LSNN-------------AMNGTIAESLGQLAELVDLNLMANTWGGVLQKSHFVNLRSLKS 485

Query: 421 NQISGPLPDNIGYQIPNIFA--FYIS----NNMHINGSLPPSVCKWRHLVFLGLSNNELS 480
            +++     ++ +++P+ +   F +      N  I G  P  +     L F+ L N  + 
Sbjct: 486 IRLTTEPYRSLVFKLPSTWIPPFRLELIQIENCRI-GLFPMWLQVQTKLNFVTLRNTGIE 545

Query: 481 GTIPSCL---LIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFD 540
            TIP      +   +T   L++N+  G  P+                 L  ++L  N+F+
Sbjct: 546 DTIPDSWFSGISSKVTYLILANNRIKGRLPQKL-----------AFPKLNTIDLSSNNFE 605

Query: 541 DTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYM 600
            T P  LW     + L L +N   G +P        + EK                    
Sbjct: 606 GTFP--LWSTNATE-LRLYENNFSGSLPQNIDVLMPRMEKI------------------- 665

Query: 601 SYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSI 660
            YL     + N+  S   V  +  + L  N+  G  P+       L  +++S NNLSG I
Sbjct: 666 -YLFSNSFTGNIPSSLCEVSGLQILSLRKNHFSGSFPKCWHRQFMLWGIDVSENNLSGEI 704

Query: 661 PVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIP 662
           P  +G    L  L L+ N L G IP ++   + L  ++L  N  +G++P
Sbjct: 726 PESLGMLPSLSVLLLNQNSLEGKIPESLRNCSGLTNIDLGGNKLTGKLP 704


HSP 3 Score: 77.0 bits (188), Expect = 5.5e-14
Identity = 123/510 (24.12%), Postives = 212/510 (41.57%), Query Frame = 1

Query: 147 KLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIP 206
           KL   + I  L  I    +LN  S A       +     +FR+ L  L      + GP  
Sbjct: 4   KLRPSFFISFLILILLLKNLNYGSAASPKCISTERQALLTFRAALTDLSSRLFSWSGP-- 63

Query: 207 IILRNMTSLRYLNLNGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSL 266
               +  +   +  +   S +V+ +    S D+  + +    +   +H  PS L +LK L
Sbjct: 64  ----DCCNWPGVLCDARTSHVVKIDLRNPSQDVRSDEYKRGSLRGKIH--PS-LTQLKFL 123

Query: 267 KSLSLRGNALHGPIPSSFGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLK 326
             L L  N           N   IP   GQ+ +L   ++S +S  G I  T   NLS+L+
Sbjct: 124 SYLDLSSNDF---------NELEIPEFIGQIVSLRYLNLSSSSFSGEIP-TSLGNLSKLE 183

Query: 327 MVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLSFPNFIYLDISNNQISG 386
            + + +             FG +G      +    L  + + L + N  Y+++S    +G
Sbjct: 184 SLDLYAES-----------FGDSGTLSLRASNLRWLSSLSSSLKYLNMGYVNLSG---AG 243

Query: 387 PLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCK---WRHLVFLGLSNNELSGTIPSCLL 446
                   +I  +   ++ N+   N  LPP++      + L  L LS N L+  IP+ L 
Sbjct: 244 ETWLQDFSRISALKELHLFNSELKN--LPPTLSSSADLKLLEVLDLSENSLNSPIPNWLF 303

Query: 447 -IPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGN-SFDDTIPFSLWI 506
            + NL   +L  +   G         SIP+   + L+ L+ L+L  N +    IP  L  
Sbjct: 304 GLTNLRKLFLRWDFLQG---------SIPTGF-KNLKLLETLDLSNNLALQGEIPSVLGD 363

Query: 507 LPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKS 566
           LP+L+ L L+ N+L G+I      F+     S V   + ++K                 +
Sbjct: 364 LPQLKFLDLSANELNGQIHGFLDAFSRNKGNSLVFLDLSSNK----------------LA 423

Query: 567 NNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLR 626
             L +S   + ++  +DLS+N+  G +P  I  + SL  L+LS+N ++G+I   +G+   
Sbjct: 424 GTLPESLGSLRNLQTLDLSSNSFTGSVPSSIGNMASLKKLDLSNNAMNGTIAESLGQLAE 452

Query: 627 LESLDLSSNQLSGSIP----LNMEKLNSLR 648
           L  L+L +N   G +     +N+  L S+R
Sbjct: 484 LVDLNLMANTWGGVLQKSHFVNLRSLKSIR 452


HSP 4 Score: 30.0 bits (66), Expect = 7.7e+00
Identity = 20/58 (34.48%), Postives = 27/58 (46.55%), Query Frame = 1

Query: 31  LSNGVDSSLFESKYLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFL 89
           ++  +   + E   L  L LS N F+   IP SFA +  L  LNLS     G+I   L
Sbjct: 821 MAGSIPEKISELSRLETLDLSKNKFSGA-IPQSFAAISSLQRLNLSFNKLEGSIPKLL 877

BLAST of Cucsa.054620 vs. TAIR10
Match: AT4G20140.1 (AT4G20140.1 Leucine-rich repeat transmembrane protein kinase)

HSP 1 Score: 208.4 bits (529), Expect = 1.6e-53
Identity = 189/639 (29.58%), Postives = 296/639 (46.32%), Query Frame = 1

Query: 53  NFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFP 112
           N+ ++T +     GL  +  LNL+     G+I P+ G    L+ LD ++   L GP    
Sbjct: 56  NYCSWTGVTCDNTGLFRVIALNLTGLGLTGSISPWFGRFDNLIHLDLSSNN-LVGPIPTA 115

Query: 113 LGELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLI----QLLNSIPSCFSLNL 172
           L  L      F + + L       L  +  ++S ++  + L+    + L ++ +   L L
Sbjct: 116 LSNLTSLESLFLFSNQLTGEIPSQLGSLVNIRSLRIGDNELVGDIPETLGNLVNLQMLAL 175

Query: 173 SSCALQNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLN-----LNGG 232
           +SC L        P       ++Q L L  N  +GPIP  L N + L         LNG 
Sbjct: 176 ASCRLTG----PIPSQLGRLVRVQSLILQDNYLEGPIPAELGNCSDLTVFTAAENMLNGT 235

Query: 233 LSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSS 292
           + + +    +++ L+L  N+   E        IPS LGE+  L+ LSL  N L G IP S
Sbjct: 236 IPAELGRLENLEILNLANNSLTGE--------IPSQLGEMSQLQYLSLMANQLQGLIPKS 295

Query: 293 FGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHD 352
             +L           NL T D+S N+L G I E  F N+SQL +  V +N HLS  +   
Sbjct: 296 LADLG----------NLQTLDLSANNLTGEIPE-EFWNMSQL-LDLVLANNHLSGSLPKS 355

Query: 353 WGFGSNGFHRWLVTQKDELGPIPTWLS-FPNFIYLDISNNQISGPLPDNIGYQIPNIFAF 412
               +    + +++     G IP  LS   +   LD+SNN ++G +P+ + +++  +   
Sbjct: 356 ICSNNTNLEQLVLSGTQLSGEIPVELSKCQSLKQLDLSNNSLAGSIPEAL-FELVELTDL 415

Query: 413 YISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCL-LIPNLTVFYLSSNKFSGVF 472
           Y+ NN  + G+L PS+    +L +L L +N L G +P  +  +  L V +L  N+FSG  
Sbjct: 416 YLHNNT-LEGTLSPSISNLTNLQWLVLYHNNLEGKLPKEISALRKLEVLFLYENRFSGEI 475

Query: 473 PR---------------NQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQ 532
           P+               N F G IP  +GR L+ L +L+L+ N     +P SL    +L 
Sbjct: 476 PQEIGNCTSLKMIDMFGNHFEGEIPPSIGR-LKELNLLHLRQNELVGGLPASLGNCHQLN 535

Query: 533 ILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLND 592
           IL LADN+L G IP     F    E+  +            +I   +  R  +  N LN 
Sbjct: 536 ILDLADNQLSGSIPS-SFGFLKGLEQLMLYNNSLQGNLPDSLISLRNLTRINLSHNRLNG 595

Query: 593 SYVHVY----SMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRL 652
           + +H      S ++ D++NN  +  IP E+    +L  L L  N L+G IP  +G+   L
Sbjct: 596 T-IHPLCGSSSYLSFDVTNNGFEDEIPLELGNSQNLDRLRLGKNQLTGKIPWTLGKIREL 655

Query: 653 ESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIP 662
             LD+SSN L+G+IPL +     L  ++L+NN  SG IP
Sbjct: 656 SLLDMSSNALTGTIPLQLVLCKKLTHIDLNNNFLSGPIP 664


HSP 2 Score: 179.5 bits (454), Expect = 7.8e-45
Identity = 202/744 (27.15%), Postives = 312/744 (41.94%), Query Frame = 1

Query: 31  LSNGVDSSLFESKYLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGN 90
           L+  + S L E   L YL L AN      IP S A L  L  L+LS+    G I     N
Sbjct: 251 LTGEIPSQLGEMSQLQYLSLMANQLQGL-IPKSLADLGNLQTLDLSANNLTGEIPEEFWN 310

Query: 91  LTKLLVLDFNNKGQLNGP-------HNFPLGELFIDGLAFRW-----ISSLRCVEYIDLS 150
           +++LL L   N   L+G        +N  L +L + G          +S  + ++ +DLS
Sbjct: 311 MSQLLDLVLANN-HLSGSLPKSICSNNTNLEQLVLSGTQLSGEIPVELSKCQSLKQLDLS 370

Query: 151 GVRVVQSGKLDVDYLIQLLNSIPSCFS--LNLSSCALQNYQLLDAPLNSSFRS--KLQHL 210
                            L  SIP      + L+   L N   L+  L+ S  +   LQ L
Sbjct: 371 NN--------------SLAGSIPEALFELVELTDLYLHN-NTLEGTLSPSISNLTNLQWL 430

Query: 211 DLSYNEFDGPIPIILRNMTSLRYLNL-----NGGLSSMVRNNCHMKSLDLLFNNFVEEMI 270
            L +N  +G +P  +  +  L  L L     +G +   + N   +K +D+  N+F  E  
Sbjct: 431 VLYHNNLEGKLPKEISALRKLEVLFLYENRFSGEIPQEIGNCTSLKMIDMFGNHFEGE-- 490

Query: 271 GFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSR--------------IPISFG 330
                 IP  +G LK L  L LR N L G +P+S GN  +              IP SFG
Sbjct: 491 ------IPPSIGRLKELNLLHLRQNELVGGLPASLGNCHQLNILDLADNQLSGSIPSSFG 550

Query: 331 QLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWL 390
            L+ L    +  NSL+G + ++   +L  L  + +S N                      
Sbjct: 551 FLKGLEQLMLYNNSLQGNLPDS-LISLRNLTRINLSHNRLN------------------- 610

Query: 391 VTQKDELGPIPTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLP 450
                  G I       +++  D++NN     +P  +G    N+    +  N  + G +P
Sbjct: 611 -------GTIHPLCGSSSYLSFDVTNNGFEDEIPLELGNS-QNLDRLRLGKNQ-LTGKIP 670

Query: 451 PSVCKWRHLVFLGLSNNELSGTIPSCLLIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMG 510
            ++ K R L  L +S+N L+GTIP  L++         +N F         SG IP W+G
Sbjct: 671 WTLGKIRELSLLDMSSNALTGTIPLQLVLCKKLTHIDLNNNF--------LSGPIPPWLG 730

Query: 511 RRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTV 570
           + L  L  L L  N F +++P  L+   +L +L L  N L G IP          E   +
Sbjct: 731 K-LSQLGELKLSSNQFVESLPTELFNCTKLLVLSLDGNSLNGSIPQ---------EIGNL 790

Query: 571 SGV-MCNSKEDQYVICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVM 630
             + + N  ++Q+             S +L  +   +  +  + LS N+L G IP EI  
Sbjct: 791 GALNVLNLDKNQF-------------SGSLPQAMGKLSKLYELRLSRNSLTGEIPVEIGQ 850

Query: 631 INSL-SNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSN 690
           +  L S L+LS+NN +G IP  IG   +LE+LDLS NQL+G +P ++  + SL  LN+S 
Sbjct: 851 LQDLQSALDLSYNNFTGDIPSTIGTLSKLETLDLSHNQLTGEVPGSVGDMKSLGYLNVSF 906

Query: 691 NNFSGRIPRG----------GNPHLCGDPFPNTCPNENGNHSDVKDKLETMWFCVIVVAG 728
           NN  G++ +           GN  LCG P  + C     N+        ++     + A 
Sbjct: 911 NNLGGKLKKQFSRWPADSFLGNTGLCGSPL-SRCNRVRSNNKQQGLSARSVVIISAISAL 906


HSP 3 Score: 173.7 bits (439), Expect = 4.3e-43
Identity = 184/652 (28.22%), Postives = 283/652 (43.40%), Query Frame = 1

Query: 59  PIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFI 118
           PIP+    L+ +  L L   Y  G I   LGN + L V     +  LNG     LG L  
Sbjct: 182 PIPSQLGRLVRVQSLILQDNYLEGPIPAELGNCSDLTVFTAA-ENMLNGTIPAELGRL-- 241

Query: 119 DGLAFRWISSLRCVEYIDLSGVRVVQSGKLD-VDYLIQLLNSIPSCFSLNLSSCALQNYQ 178
           + L    +++        L+G    Q G++  + YL  + N +      +L+   L N Q
Sbjct: 242 ENLEILNLANN------SLTGEIPSQLGEMSQLQYLSLMANQLQGLIPKSLAD--LGNLQ 301

Query: 179 LLDAPLNSSF---------RSKLQHLDLSYNEFDGPIP-IILRNMTSLRYLNLNG----G 238
            LD   N+            S+L  L L+ N   G +P  I  N T+L  L L+G    G
Sbjct: 302 TLDLSANNLTGEIPEEFWNMSQLLDLVLANNHLSGSLPKSICSNNTNLEQLVLSGTQLSG 361

Query: 239 LSSMVRNNCH-MKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPS 298
              +  + C  +K LDL  N+           +IP  L EL  L  L L  N L G +  
Sbjct: 362 EIPVELSKCQSLKQLDLSNNSLAG--------SIPEALFELVELTDLYLHNNTLEGTLSP 421

Query: 299 SFGNLS--------------RIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMV 358
           S  NL+              ++P     LR L    + +N   G I +    N + LKM+
Sbjct: 422 SISNLTNLQWLVLYHNNLEGKLPKEISALRKLEVLFLYENRFSGEIPQ-EIGNCTSLKMI 481

Query: 359 AVSSNEHLSFEIKHDWGFGS--NGFHRWLVTQKDELGPIPTWLSFPNFI-YLDISNNQIS 418
            +  N H   EI    G     N  H   + Q + +G +P  L   + +  LD+++NQ+S
Sbjct: 482 DMFGN-HFEGEIPPSIGRLKELNLLH---LRQNELVGGLPASLGNCHQLNILDLADNQLS 541

Query: 419 GPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLLIP 478
           G +P + G+ +  +    + NN  + G+LP S+   R+L  + LS+N L+GTI       
Sbjct: 542 GSIPSSFGF-LKGLEQLMLYNNS-LQGNLPDSLISLRNLTRINLSHNRLNGTIHPLCGSS 601

Query: 479 NLTVFYLSSNKFSGVFP---------------RNQFSGSIPSWMGRRLQSLQILNLQGNS 538
           +   F +++N F    P               +NQ +G IP W   +++ L +L++  N+
Sbjct: 602 SYLSFDVTNNGFEDEIPLELGNSQNLDRLRLGKNQLTGKIP-WTLGKIRELSLLDMSSNA 661

Query: 539 FDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVIC 598
              TIP  L +  +L  + L +N L G IPP   K +   E    S     S   +   C
Sbjct: 662 LTGTIPLQLVLCKKLTHIDLNNNFLSGPIPPWLGKLSQLGELKLSSNQFVESLPTELFNC 721

Query: 599 YMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSG 658
                                  ++ + L  N+L G IP+EI  + +L+ LNL  N  SG
Sbjct: 722 T---------------------KLLVLSLDGNSLNGSIPQEIGNLGALNVLNLDKNQFSG 781

Query: 659 SIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLR-ALNLSNNNFSGRIP 662
           S+P  +G+  +L  L LS N L+G IP+ + +L  L+ AL+LS NNF+G IP
Sbjct: 782 SLPQAMGKLSKLYELRLSRNSLTGEIPVEIGQLQDLQSALDLSYNNFTGDIP 785

BLAST of Cucsa.054620 vs. TAIR10
Match: AT5G44700.1 (AT5G44700.1 Leucine-rich repeat transmembrane protein kinase)

HSP 1 Score: 204.9 bits (520), Expect = 1.7e-52
Identity = 192/640 (30.00%), Postives = 299/640 (46.72%), Query Frame = 1

Query: 51  SANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHN 110
           S ++ N+T +      ++GL   NLS     G+I P +G    L+ +D ++  +L GP  
Sbjct: 57  SPSYCNWTGVTCGGREIIGL---NLSGLGLTGSISPSIGRFNNLIHIDLSSN-RLVGPIP 116

Query: 111 FPLGELFIDGLAFRWISSLRCVEYIDLSGVRV-VQSGKLDVDYLIQLLNSIPSCFS--LN 170
             L  L     +    S+L   +     G  V ++S KL  +   +L  +IP  F   +N
Sbjct: 117 TTLSNLSSSLESLHLFSNLLSGDIPSQLGSLVNLKSLKLGDN---ELNGTIPETFGNLVN 176

Query: 171 LSSCALQNYQLLDA-PLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLR-----YLNLN 230
           L   AL + +L    P       +LQ L L  NE +GPIP  + N TSL      +  LN
Sbjct: 177 LQMLALASCRLTGLIPSRFGRLVQLQTLILQDNELEGPIPAEIGNCTSLALFAAAFNRLN 236

Query: 231 GGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIP 290
           G L + +    ++++L+L  N+F  E        IPS LG+L S++ L+L GN L G IP
Sbjct: 237 GSLPAELNRLKNLQTLNLGDNSFSGE--------IPSQLGDLVSIQYLNLIGNQLQGLIP 296

Query: 291 SSFGNLSRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIK 350
                L+          NL T D+S N+L GVI E  F  ++QL+ + ++ N  LS  + 
Sbjct: 297 KRLTELA----------NLQTLDLSSNNLTGVIHE-EFWRMNQLEFLVLAKNR-LSGSLP 356

Query: 351 HDWGFGSNGFHRWLVTQKDELGPIPTWLS-FPNFIYLDISNNQISGPLPDNIGYQIPNIF 410
                 +    +  +++    G IP  +S   +   LD+SNN ++G +PD++ +Q+  + 
Sbjct: 357 KTICSNNTSLKQLFLSETQLSGEIPAEISNCQSLKLLDLSNNTLTGQIPDSL-FQLVELT 416

Query: 411 AFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCL-LIPNLTVFYLSSNKFSG 470
             Y++NN  + G+L  S+    +L    L +N L G +P  +  +  L + YL  N+FSG
Sbjct: 417 NLYLNNNS-LEGTLSSSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKLEIMYLYENRFSG 476

Query: 471 VFP---------------RNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPR 530
             P                N+ SG IPS +GR L+ L  L+L+ N     IP SL    +
Sbjct: 477 EMPVEIGNCTRLQEIDWYGNRLSGEIPSSIGR-LKDLTRLHLRENELVGNIPASLGNCHQ 536

Query: 531 LQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNL 590
           + ++ LADN+L G IP     F T  E   +            +I   +  R    SN  
Sbjct: 537 MTVIDLADNQLSGSIPS-SFGFLTALELFMIYNNSLQGNLPDSLINLKNLTRINFSSNKF 596

Query: 591 NDSYVHVY---SMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLR 650
           N S   +    S ++ D++ N  +G IP E+    +L  L L  N  +G IP   G+   
Sbjct: 597 NGSISPLCGSSSYLSFDVTENGFEGDIPLELGKSTNLDRLRLGKNQFTGRIPRTFGKISE 656

Query: 651 LESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIP 662
           L  LD+S N LSG IP+ +     L  ++L+NN  SG IP
Sbjct: 657 LSLLDISRNSLSGIIPVELGLCKKLTHIDLNNNYLSGVIP 665


HSP 2 Score: 166.4 bits (420), Expect = 6.9e-41
Identity = 197/690 (28.55%), Postives = 290/690 (42.03%), Query Frame = 1

Query: 32  SNGVDSSLFESKYLNYLGLSANFFNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNL 91
           S  + S L +   + YL L  N      IP     L  L  L+LSS    G I      +
Sbjct: 253 SGEIPSQLGDLVSIQYLNLIGNQLQGL-IPKRLTELANLQTLDLSSNNLTGVIHEEFWRM 312

Query: 92  TKLLVLDFNNKGQLNGP-------HNFPLGELFID-----GLAFRWISSLRCVEYIDLSG 151
            +L  L    K +L+G        +N  L +LF+      G     IS+ + ++ +DLS 
Sbjct: 313 NQLEFLVLA-KNRLSGSLPKTICSNNTSLKQLFLSETQLSGEIPAEISNCQSLKLLDLSN 372

Query: 152 VRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLLDAPLNSSFRS--KLQHLDLS 211
             +  +G++  D L QL+          L++  L N  L +  L+SS  +   LQ   L 
Sbjct: 373 NTL--TGQIP-DSLFQLVE---------LTNLYLNNNSL-EGTLSSSISNLTNLQEFTLY 432

Query: 212 YNEFDGPIPIILRNMTSLRYLNL-----NGGLSSMVRNNCHMKSLDLLFNNFVEEMIGFG 271
           +N  +G +P  +  +  L  + L     +G +   + N   ++ +D   N    E     
Sbjct: 433 HNNLEGKVPKEIGFLGKLEIMYLYENRFSGEMPVEIGNCTRLQEIDWYGNRLSGE----- 492

Query: 272 MHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSR--------------IPISFGQLR 331
              IPS +G LK L  L LR N L G IP+S GN  +              IP SFG L 
Sbjct: 493 ---IPSSIGRLKDLTRLHLRENELVGNIPASLGNCHQMTVIDLADNQLSGSIPSSFGFLT 552

Query: 332 NLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQ 391
            L  F +  NSL+G + ++   NL  L  +                 F SN F+      
Sbjct: 553 ALELFMIYNNSLQGNLPDS-LINLKNLTRI----------------NFSSNKFN------ 612

Query: 392 KDELGPIPTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSV 451
               G I       +++  D++ N   G +P  +G +  N+    +  N    G +P + 
Sbjct: 613 ----GSISPLCGSSSYLSFDVTENGFEGDIPLELG-KSTNLDRLRLGKNQ-FTGRIPRTF 672

Query: 452 CKWRHLVFLGLSNNELSGTIPSCL-LIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMGRR 511
            K   L  L +S N LSG IP  L L   LT   L++N  SGV         IP+W+G+ 
Sbjct: 673 GKISELSLLDISRNSLSGIIPVELGLCKKLTHIDLNNNYLSGV---------IPTWLGK- 732

Query: 512 LQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPP----IEAKFATKFEKS 571
           L  L  L L  N F  ++P  ++ L  +  L L  N L G IP     ++A  A   E++
Sbjct: 733 LPLLGELKLSSNKFVGSLPTEIFSLTNILTLFLDGNSLNGSIPQEIGNLQALNALNLEEN 792

Query: 572 TVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIV 631
            +SG +             S + ++ K             +  + LS N L G IP EI 
Sbjct: 793 QLSGPL------------PSTIGKLSK-------------LFELRLSRNALTGEIPVEIG 852

Query: 632 MINSL-SNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLS 673
            +  L S L+LS+NN +G IP  I    +LESLDLS NQL G +P  +  + SL  LNLS
Sbjct: 853 QLQDLQSALDLSYNNFTGRIPSTISTLPKLESLDLSHNQLVGEVPGQIGDMKSLGYLNLS 855


HSP 3 Score: 165.2 bits (417), Expect = 1.5e-40
Identity = 177/654 (27.06%), Postives = 285/654 (43.58%), Query Frame = 1

Query: 60  IPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLV-------LDFNNKGQLNGPHNFP 119
           IP+ F  L+ L  L L      G I   +GN T L +       L+ +   +LN   N  
Sbjct: 184 IPSRFGRLVQLQTLILQDNELEGPIPAEIGNCTSLALFAAAFNRLNGSLPAELNRLKNLQ 243

Query: 120 ---LGELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLS 179
              LG+    G     +  L  ++Y++L G ++          + + L  + +  +L+LS
Sbjct: 244 TLNLGDNSFSGEIPSQLGDLVSIQYLNLIGNQLQ-------GLIPKRLTELANLQTLDLS 303

Query: 180 SCALQNYQLLDAPLNSSF--RSKLQHLDLSYNEFDGPIP-IILRNMTSLRYL-----NLN 239
           S        L   ++  F   ++L+ L L+ N   G +P  I  N TSL+ L      L+
Sbjct: 304 S------NNLTGVIHEEFWRMNQLEFLVLAKNRLSGSLPKTICSNNTSLKQLFLSETQLS 363

Query: 240 GGLSSMVRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIP 299
           G + + + N   +K LDL  N    +        IP  L +L  L +L L  N+L G + 
Sbjct: 364 GEIPAEISNCQSLKLLDLSNNTLTGQ--------IPDSLFQLVELTNLYLNNNSLEGTLS 423

Query: 300 SSFGNLS--------------RIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKM 359
           SS  NL+              ++P   G L  L    + +N   G +      N ++L+ 
Sbjct: 424 SSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKLEIMYLYENRFSGEMP-VEIGNCTRLQE 483

Query: 360 VAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWL-SFPNFIYLDISNNQISG 419
           +    N  LS EI    G       R  + + + +G IP  L +      +D+++NQ+SG
Sbjct: 484 IDWYGNR-LSGEIPSSIG-RLKDLTRLHLRENELVGNIPASLGNCHQMTVIDLADNQLSG 543

Query: 420 PLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLLIPN 479
            +P + G+ +  +  F I NN  + G+LP S+   ++L  +  S+N+ +G+I       +
Sbjct: 544 SIPSSFGF-LTALELFMIYNNS-LQGNLPDSLINLKNLTRINFSSNKFNGSISPLCGSSS 603

Query: 480 LTVFYLSSNKFSGVFP---------------RNQFSGSIPSWMGRRLQSLQILNLQGNSF 539
              F ++ N F G  P               +NQF+G IP   G+ +  L +L++  NS 
Sbjct: 604 YLSFDVTENGFEGDIPLELGKSTNLDRLRLGKNQFTGRIPRTFGK-ISELSLLDISRNSL 663

Query: 540 DDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICY 599
              IP  L +  +L  + L +N L G IP    K     E    S     S   +     
Sbjct: 664 SGIIPVELGLCKKLTHIDLNNNYLSGVIPTWLGKLPLLGELKLSSNKFVGSLPTE----- 723

Query: 600 MSYLRQVMKSNNLNDSYVHVYSMVNV---DLSNNNLQGHIPREIVMINSLSNLNLSHNNL 659
                              ++S+ N+    L  N+L G IP+EI  + +L+ LNL  N L
Sbjct: 724 -------------------IFSLTNILTLFLDGNSLNGSIPQEIGNLQALNALNLEENQL 783

Query: 660 SGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLR-ALNLSNNNFSGRIP 662
           SG +P  IG+  +L  L LS N L+G IP+ + +L  L+ AL+LS NNF+GRIP
Sbjct: 784 SGPLPSTIGKLSKLFELRLSRNALTGEIPVEIGQLQDLQSALDLSYNNFTGRIP 786

BLAST of Cucsa.054620 vs. TAIR10
Match: AT3G28890.1 (AT3G28890.1 receptor like protein 43)

HSP 1 Score: 203.8 bits (517), Expect = 3.9e-52
Identity = 162/522 (31.03%), Postives = 241/522 (46.17%), Query Frame = 1

Query: 189 SKLQHLDLSYNEFDGPIPIILRNMTSLRYLNL-----NGGLSSMVRNNCHMKSLDLLFNN 248
           S L +LDLS+N F G +P  + N++ L +L+L     +G + S + N  H+ +L+L FN 
Sbjct: 145 SHLTYLDLSFNHFSGQVPSSIGNLSHLTFLDLYCNQFSGQVPSSIGNLSHLTTLELSFNR 204

Query: 249 FVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSRIPISFGQLRNLNTF 308
           F      FG    PS +G L  L +L+L  N   G IPSS GNLS          NL + 
Sbjct: 205 F------FGQ--FPSSIGGLSHLTTLNLFVNNFLGQIPSSIGNLS----------NLTSL 264

Query: 309 DVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWLVTQKDELG 368
            + KN+  G I  +   NLSQL  + +SSN                             G
Sbjct: 265 YLCKNNFSGQIP-SFIGNLSQLTRLDLSSNNFF--------------------------G 324

Query: 369 PIPTWL-SFPNFIYLDISNNQISGPLPDNIGYQIPN----IFAFYISNNMHINGSLPPSV 428
            IP WL + PN  Y+++S N         IG+Q PN         + +N +  G +P  +
Sbjct: 325 EIPGWLWTLPNLFYVNLSYNTF-------IGFQRPNKPEPSMGHLLGSNNNFTGKIPSFI 384

Query: 429 CKWRHLVFLGLSNNELSGTIPSCL--LIPNLTVFYLSSNKFSGVFPR------------- 488
           C+ R L  L LS+N  SG IP C+  L  NL+   L  N  SG  P+             
Sbjct: 385 CELRSLETLDLSDNNFSGLIPRCMGNLKSNLSHLNLRQNNLSGGLPKHIFEILRSLDVGH 444

Query: 489 NQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIE 548
           NQ  G +P  + R   +L++LN++ N  +DT PF L  LP+LQ+L+L  N   G I   E
Sbjct: 445 NQLVGKLPRSL-RFFSTLEVLNVESNRINDTFPFWLTSLPKLQVLVLRSNAFHGPIH--E 504

Query: 549 AKFAT----KFEKSTVSGVM-------------CNSKEDQYVICYMSYL----RQVMKSN 608
           A F          +  +G +               + ED+    YM  +      V+ + 
Sbjct: 505 ASFLKLRIIDISHNHFNGTLPSDYFVKWSAMSSLGTDEDRSNANYMGSVYYQDSMVLMNK 564

Query: 609 NLNDSYVHVYSMVN-VDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLR 664
            +    + + ++   +D S N  +G IP+ I ++  L  LNLS+N  +G IP  +G+   
Sbjct: 565 GVESELIRILTIYTALDFSGNKFEGEIPKSIGLLKELLVLNLSNNAFTGHIPSSMGKLTA 611


HSP 2 Score: 104.4 bits (259), Expect = 3.2e-22
Identity = 156/546 (28.57%), Postives = 229/546 (41.94%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEYFYSSAFLSNGVDSSLFESKYLNYLGLSANFFNYTPI 60
           W+GV C+    G V++LDL +  Y +   F SN   SS+    +L  L LS N F    I
Sbjct: 85  WEGVTCN-AKSGEVIELDL-SCSYLHGR-FHSN---SSIRNLHFLTTLDLSFNDFK-GQI 144

Query: 61  PNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDG 120
            +S   L  LTYL+LS  +F G +   +GNL+ L  LD     Q +G     +G L    
Sbjct: 145 MSSIENLSHLTYLDLSFNHFSGQVPSSIGNLSHLTFLDLYCN-QFSGQVPSSIGNL--SH 204

Query: 121 LAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFS--LNLSSCAL-QNYQ 180
           L    +S  R       S   +     L++ ++   L  IPS      NL+S  L +N  
Sbjct: 205 LTTLELSFNRFFGQFPSSIGGLSHLTTLNL-FVNNFLGQIPSSIGNLSNLTSLYLCKNNF 264

Query: 181 LLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLN-GGLSSMVRNNCHMKS 240
               P      S+L  LDLS N F G IP  L  + +L Y+NL+        R N    S
Sbjct: 265 SGQIPSFIGNLSQLTRLDLSSNNFFGEIPGWLWTLPNLFYVNLSYNTFIGFQRPNKPEPS 324

Query: 241 LDLLF---NNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSRIPIS 300
           +  L    NNF  +        IPS++ EL+SL++L L  N   G IP   GNL      
Sbjct: 325 MGHLLGSNNNFTGK--------IPSFICELRSLETLDLSDNNFSGLIPRCMGNLKS---- 384

Query: 301 FGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHR 360
                NL+  ++ +N+L G + +  F  L  L +     +  L  ++     F S     
Sbjct: 385 -----NLSHLNLRQNNLSGGLPKHIFEILRSLDV----GHNQLVGKLPRSLRFFST--LE 444

Query: 361 WLVTQKDELGP-IPTWL-SFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHIN 420
            L  + + +    P WL S P    L + +N   GP+ +    ++  I    IS+N H N
Sbjct: 445 VLNVESNRINDTFPFWLTSLPKLQVLVLRSNAFHGPIHEASFLKLRIID---ISHN-HFN 504

Query: 421 GSLPPSV-CKWRHLVFLGLSNNELSGTIPSCLLIPNLTVFYLSSNKFSGVFPRNQFSGSI 480
           G+LP     KW  +  LG   +  +            +V+Y  S            +  +
Sbjct: 505 GTLPSDYFVKWSAMSSLGTDEDRSNANYMG-------SVYYQDSMVL--------MNKGV 564

Query: 481 PSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATKF 537
            S + R L     L+  GN F+  IP S+ +L  L +L L++N   G IP    K  T  
Sbjct: 565 ESELIRILTIYTALDFSGNKFEGEIPKSIGLLKELLVLNLSNNAFTGHIPSSMGKL-TAL 576


HSP 3 Score: 95.9 bits (237), Expect = 1.1e-19
Identity = 163/655 (24.89%), Postives = 253/655 (38.63%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEYFYSSAFLSNGVDSSLFESKYLNYLGLSANFFNYTPI 60
           W+GV C+    G V++LDL       S ++L     S+                      
Sbjct: 85  WEGVTCN-AKSGEVIELDL-------SCSYLHGRFHSN---------------------- 144

Query: 61  PNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLD--FNN-KGQLNGPHNFPLGELF 120
            +S   L  LT L+LS   F G I   + NL+ L  LD  FN+  GQ+            
Sbjct: 145 -SSIRNLHFLTTLDLSFNDFKGQIMSSIENLSHLTYLDLSFNHFSGQVPSS--------- 204

Query: 121 IDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFS--LNLSSCALQ- 180
                   I +L  + ++DL              Y  Q    +PS      +L++  L  
Sbjct: 205 --------IGNLSHLTFLDL--------------YCNQFSGQVPSSIGNLSHLTTLELSF 264

Query: 181 NYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYL-----NLNGGLSSMVR 240
           N      P +    S L  L+L  N F G IP  + N+++L  L     N +G + S + 
Sbjct: 265 NRFFGQFPSSIGGLSHLTTLNLFVNNFLGQIPSSIGNLSNLTSLYLCKNNFSGQIPSFIG 324

Query: 241 NNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHG------PIPSS 300
           N   +  LDL  NNF  E        IP WL  L +L  ++L  N   G      P PS 
Sbjct: 325 NLSQLTRLDLSSNNFFGE--------IPGWLWTLPNLFYVNLSYNTFIGFQRPNKPEPS- 384

Query: 301 FGNL--------SRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEH 360
            G+L         +IP    +LR+L T D+S N+  G+I       +  LK    S+  H
Sbjct: 385 MGHLLGSNNNFTGKIPSFICELRSLETLDLSDNNFSGLIPRC----MGNLK----SNLSH 444

Query: 361 LSFEIKHDWGFGSNGFHRWLVTQKDELGPIPTWLSFPNFIYLDISNNQISGPLPDNIGYQ 420
           L+                    Q +  G +P  + F     LD+ +NQ+ G LP ++ + 
Sbjct: 445 LNLR------------------QNNLSGGLPKHI-FEILRSLDVGHNQLVGKLPRSLRF- 504

Query: 421 IPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLLIPNLTVFYLSSN 480
              +    + +N  IN + P  +     L  L L +N   G I     +  L +  +S N
Sbjct: 505 FSTLEVLNVESN-RINDTFPFWLTSLPKLQVLVLRSNAFHGPIHEASFL-KLRIIDISHN 564

Query: 481 KFSGVFPRNQF-SGSIPSWMGRRLQSLQILNLQGNSFDDTIPFS--------LWILPRLQ 540
            F+G  P + F   S  S +G          +    + D++           + IL    
Sbjct: 565 HFNGTLPSDYFVKWSAMSSLGTDEDRSNANYMGSVYYQDSMVLMNKGVESELIRILTIYT 609

Query: 541 ILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLND 600
            L  + NK EGEIP           KS                  +  L++++  N  N+
Sbjct: 625 ALDFSGNKFEGEIP-----------KS------------------IGLLKELLVLNLSNN 609

Query: 601 SYV-HVYSMV-------NVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIP 614
           ++  H+ S +       ++D+S N L G IP+EI  ++ LS +N SHN L+G +P
Sbjct: 685 AFTGHIPSSMGKLTALESLDVSQNKLYGEIPQEIGNLSFLSCMNFSHNQLAGLVP 609


HSP 4 Score: 88.6 bits (218), Expect = 1.8e-17
Identity = 90/312 (28.85%), Postives = 128/312 (41.03%), Query Frame = 1

Query: 375 IYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNEL 434
           I LD+S + + G    N   +  +       +     G +  S+    HL +L LS N  
Sbjct: 98  IELDLSCSYLHGRFHSNSSIRNLHFLTTLDLSFNDFKGQIMSSIENLSHLTYLDLSFNHF 157

Query: 435 SGTIPSCLLIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDT 494
           SG +PS       ++  LS   F  ++  NQFSG +PS +G  L  L  L L  N F   
Sbjct: 158 SGQVPS-------SIGNLSHLTFLDLYC-NQFSGQVPSSIGN-LSHLTTLELSFNRFFGQ 217

Query: 495 IPFSLWILPRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSY 554
            P S+  L  L  L L  N   G+IP      +     +  S  +C +     +  ++  
Sbjct: 218 FPSSIGGLSHLTTLNLFVNNFLGQIPSSIGNLS-----NLTSLYLCKNNFSGQIPSFIGN 277

Query: 555 LRQVMKSNNLNDSYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHN-------- 614
           L Q+ +                +DLS+NN  G IP  +  + +L  +NLS+N        
Sbjct: 278 LSQLTR----------------LDLSSNNFFGEIPGWLWTLPNLFYVNLSYNTFIGFQRP 337

Query: 615 ---------------NLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNS-LRALN 663
                          N +G IP  I     LE+LDLS N  SG IP  M  L S L  LN
Sbjct: 338 NKPEPSMGHLLGSNNNFTGKIPSFICELRSLETLDLSDNNFSGLIPRCMGNLKSNLSHLN 379


HSP 5 Score: 68.6 bits (166), Expect = 1.9e-11
Identity = 56/181 (30.94%), Postives = 82/181 (45.30%), Query Frame = 1

Query: 537 GVMCNSKEDQYVICYMS--YLRQVMKSNN--------------LNDSYVHVYSMVN---- 596
           GV CN+K  + +   +S  YL     SN+               ND    + S +     
Sbjct: 87  GVTCNAKSGEVIELDLSCSYLHGRFHSNSSIRNLHFLTTLDLSFNDFKGQIMSSIENLSH 146

Query: 597 ---VDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLS 656
              +DLS N+  G +P  I  ++ L+ L+L  N  SG +P  IG    L +L+LS N+  
Sbjct: 147 LTYLDLSFNHFSGQVPSSIGNLSHLTFLDLYCNQFSGQVPSSIGNLSHLTTLELSFNRFF 206

Query: 657 GSIPLNMEKLNSLRALNLSNNNFSGRIPRG-GN------PHLCGDPFPNTCPNENGNHSD 688
           G  P ++  L+ L  LNL  NNF G+IP   GN       +LC + F    P+  GN S 
Sbjct: 207 GQFPSSIGGLSHLTTLNLFVNNFLGQIPSSIGNLSNLTSLYLCKNNFSGQIPSFIGNLSQ 266

BLAST of Cucsa.054620 vs. TAIR10
Match: AT1G74170.1 (AT1G74170.1 receptor like protein 13)

HSP 1 Score: 202.2 bits (513), Expect = 1.1e-51
Identity = 234/816 (28.68%), Postives = 349/816 (42.77%), Query Frame = 1

Query: 18  DLRNYEYFYSSAFLSNGVDS--SLFESKYLNYLGLSANFFNYT-PIPNSFAGLLGLT--- 77
           DL N E    S    NG     +LF  + L  L LS N F+ +  +   FA    L+   
Sbjct: 196 DLTNVELLDLSRNRFNGSIPVRALFALRKLKALDLSDNEFSSSVELQGKFAKTKPLSGTC 255

Query: 78  ------YLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDGLAFRW 137
                  L LS+    G     L +LT L VLD ++  QL G  N P             
Sbjct: 256 PWKNMEELKLSNNKLAGQFPLCLTSLTGLRVLDLSSN-QLTG--NVPSA----------- 315

Query: 138 ISSLRCVEYIDLSGVR---------VVQSGKLDVDYLIQLLNSIPSCFSLN------LSS 197
           +++L  +EY+ L G           +    KL V  L    NS+   F  +      L  
Sbjct: 316 LANLESLEYLSLFGNNFEGFFSLGLLANLSKLKVLRLDSQSNSLEVEFETSWKPKFQLVV 375

Query: 198 CALQNYQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPI-ILRNMTSLRYLNL--NGGLSSM 257
            AL++  L   P     +  L H+DLS N+  G  P  +L N T L  L L  N   S  
Sbjct: 376 IALRSCNLEKVPHFLLHQKDLHHVDLSDNQIHGNFPSWLLENNTKLEVLLLQNNSFTSFQ 435

Query: 258 VRNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNL 317
           +  + H    +LLF N               W+  L  L  ++L  N   G +PSS  N+
Sbjct: 436 LPKSAH----NLLFLNVSVNKFNHLFLQNFGWI--LPHLVCVNLAYNGFQGNLPSSLDNM 495

Query: 318 SRIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFG 377
                     +++   D+S N   G +          L ++ +S N+ LS E+  +    
Sbjct: 496 ----------KSIEFLDLSHNRFHGKLPRRFLKGCYNLTILKLSHNK-LSGEVFPE---A 555

Query: 378 SNGFHRWLVTQKDEL--GPIPT-WLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYI 437
           +N    W+++  + L  G I   + S P+   LDISNN+++G +P  IG +   +FA  +
Sbjct: 556 ANFTRLWVMSMDNNLFTGNIGKGFRSLPSLNVLDISNNKLTGVIPSWIGER-QGLFALQL 615

Query: 438 SNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLL-IPNLTVFYLSSNKFSGVFP- 497
           SNNM + G +P S+    +L  L LS+N LSG IP  +  I +  V  L +N  SGV P 
Sbjct: 616 SNNM-LEGEIPTSLFNISYLQLLDLSSNRLSGDIPPHVSSIYHGAVLLLQNNNLSGVIPD 675

Query: 498 ------------RNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILIL 557
                        N+ SG++P ++    Q++ IL L+GN+F   IP     L  +Q+L L
Sbjct: 676 TLLLNVIVLDLRNNRLSGNLPEFINT--QNISILLLRGNNFTGQIPHQFCSLSNIQLLDL 735

Query: 558 ADNKLEGEIPPIEAKFATKFEKST------VSGVMCNSKEDQYV--ICYMSYLRQVMKSN 617
           ++NK  G IP   +  +    K        V      +K+  Y   +  +     V ++N
Sbjct: 736 SNNKFNGSIPSCLSNTSFGLRKGDDSYRYDVPSRFGTAKDPVYFESLLMIDEFNMVNETN 795

Query: 618 NLN----------DSYV--HVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSG 677
           +            D+Y+  ++  +  +DLS N L G IP E+  +  L  LNLSHNNLSG
Sbjct: 796 SQTKIEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVELGGLVELEALNLSHNNLSG 855

Query: 678 SIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIPRG------- 737
            I         +ESLDLS N+L G IPL +  + SL   N+S NN SG +P+G       
Sbjct: 856 VILESFSGLKNVESLDLSFNRLQGPIPLQLTDMISLAVFNVSYNNLSGIVPQGRQFNTFE 915

Query: 738 -----GNPHLCGDPFPNTC------PNENGNHSDVKDKLETMWFCVIVVAGYSFGFWGVV 749
                GNP LCG     +C      P +NG  +D +  ++   F    VA Y     G++
Sbjct: 916 TQSYFGNPLLCGKSIDISCASNNFHPTDNGVEAD-ESTVDMESFYWSFVAAYVTILLGIL 972


HSP 2 Score: 148.3 bits (373), Expect = 1.9e-35
Identity = 179/650 (27.54%), Postives = 277/650 (42.62%), Query Frame = 1

Query: 45  LNYLGLSANFFNYTPIP----NSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDF- 104
           L +L L  N FN + IP    NS      L  L+LS   F+  I PFL + T L  L   
Sbjct: 123 LEHLDLRGNRFNGS-IPTQDYNSLRRFRKLEILDLSDNLFNSRIFPFLNSATSLKSLSLW 182

Query: 105 -NNKGQLNGPHNFPLGEL---------------FIDGLAFRWISSLRCVEYIDLSGVRVV 164
            NN G   GP  FP  EL               F   +  R + +LR ++ +DLS     
Sbjct: 183 GNNMG---GP--FPAKELRDLTNVELLDLSRNRFNGSIPVRALFALRKLKALDLSDNEFS 242

Query: 165 QSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLLDA-PLNSSFRSKLQHLDLSYNEFD 224
            S +L   +      S  +C   N+    L N +L    PL  +  + L+ LDLS N+  
Sbjct: 243 SSVELQGKFAKTKPLS-GTCPWKNMEELKLSNNKLAGQFPLCLTSLTGLRVLDLSSNQLT 302

Query: 225 GPIPIILRNMTSLRYLNLNGG-------------LSSM--VRNNCHMKSLDLLFNN---- 284
           G +P  L N+ SL YL+L G              LS +  +R +    SL++ F      
Sbjct: 303 GNVPSALANLESLEYLSLFGNNFEGFFSLGLLANLSKLKVLRLDSQSNSLEVEFETSWKP 362

Query: 285 ----FVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPS-SFGNLSRIPISFGQLR 344
                V  +    +  +P +L   K L  + L  N +HG  PS    N +++ +   Q  
Sbjct: 363 KFQLVVIALRSCNLEKVPHFLLHQKDLHHVDLSDNQIHGNFPSWLLENNTKLEVLLLQNN 422

Query: 345 NLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDW--------GFGSNG 404
           +  +F + K++           NL  L  V+V+   HL  +    W            NG
Sbjct: 423 SFTSFQLPKSA----------HNLLFLN-VSVNKFNHLFLQ-NFGWILPHLVCVNLAYNG 482

Query: 405 FHRWLVTQKDELGPIPTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHI 464
           F   L +  D +  I          +LD+S+N+  G LP        N+    +S+N  +
Sbjct: 483 FQGNLPSSLDNMKSIE---------FLDLSHNRFHGKLPRRFLKGCYNLTILKLSHNK-L 542

Query: 465 NGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLL-IPNLTVFYLSSNKFSGVFPRNQFSGS 524
           +G + P    +  L  + + NN  +G I      +P+L V  +S+NK +GV         
Sbjct: 543 SGEVFPEAANFTRLWVMSMDNNLFTGNIGKGFRSLPSLNVLDISNNKLTGV--------- 602

Query: 525 IPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIEAKFATK 584
           IPSW+G R Q L  L L  N  +  IP SL+ +  LQ+L L+ N+L G+IPP        
Sbjct: 603 IPSWIGER-QGLFALQLSNNMLEGEIPTSLFNISYLQLLDLSSNRLSGDIPP-------- 662

Query: 585 FEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDSY--VHVYSMVNVDLSNNNLQGH 638
              S   G +                  ++++NNL+       + +++ +DL NN L G+
Sbjct: 663 HVSSIYHGAVL-----------------LLQNNNLSGVIPDTLLLNVIVLDLRNNRLSGN 707

BLAST of Cucsa.054620 vs. NCBI nr
Match: gi|659068439|ref|XP_008444420.1| (PREDICTED: leucine-rich repeat receptor protein kinase EXS-like [Cucumis melo])

HSP 1 Score: 783.5 bits (2022), Expect = 3.4e-223
Identity = 442/763 (57.93%), Postives = 494/763 (64.74%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEYFYSSAFLSNGVDSSLFESKYLNYLGLSANFFNYTPI 60
           W+GVGCD  NGGHVVKLDLRNYEYFYSSA LSNGVDSSLFE KYLNYL LS NFFNYT I
Sbjct: 64  WEGVGCDRANGGHVVKLDLRNYEYFYSSALLSNGVDSSLFELKYLNYLDLSGNFFNYTQI 123

Query: 61  PNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDG 120
           PNSFA LL LTYLNLSSTYFHG IQPF GNLTKLLVLDFNN+  LNGP  FPLGELFIDG
Sbjct: 124 PNSFAELLELTYLNLSSTYFHGTIQPFPGNLTKLLVLDFNNEENLNGPDYFPLGELFIDG 183

Query: 121 LAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLLD 180
           LA RW+S L C+EY+ LSGVRVVQSGKL VDYLIQLLN++PS  SL LSSCALQN  LL 
Sbjct: 184 LALRWVSGLLCLEYLHLSGVRVVQSGKLGVDYLIQLLNTVPSLLSLKLSSCALQNDLLLY 243

Query: 181 APLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLR------------------------ 240
           APLNSSF SKLQHLDLSYNEFDGPIPIIL+NMTSL+                        
Sbjct: 244 APLNSSFLSKLQHLDLSYNEFDGPIPIILQNMTSLKYLNLNGNLYNSSIPSWLSNLRNLD 303

Query: 241 --------YLNLNGGLSSMVRNNCHMKSLDLLFNNFV----------------------- 300
                   + ++ GG  SMVRNNCH+KSLDL FN+FV                       
Sbjct: 304 TLSLGSNSFSSIEGGFPSMVRNNCHLKSLDLSFNHFVGEDVFGSYENLSSGCKEYGLQRL 363

Query: 301 --EEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSR-------------- 360
             E MI FG HTIPSWLGELK+LKSLSLR NAL+GPIPSSFGNLS               
Sbjct: 364 YLERMIRFGTHTIPSWLGELKNLKSLSLRENALYGPIPSSFGNLSSLEDLDISYNRLRGG 423

Query: 361 IPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDW----- 420
           IPISFGQLRNLN+ DVSKNSLKGVITETHFANLSQLKMVA+SSNEHLSFEIKHDW     
Sbjct: 424 IPISFGQLRNLNSLDVSKNSLKGVITETHFANLSQLKMVAMSSNEHLSFEIKHDWVPPFQ 483

Query: 421 ----------GFGSNGFHRWLVTQKDEL------------GPIPTWLSFPNFIYLDISNN 480
                     GFGSNGF RWLVTQKDEL            GPIPTWLSFPNFIYLDISNN
Sbjct: 484 LQYFSVRSTKGFGSNGFPRWLVTQKDELVLYLVLSNTSLSGPIPTWLSFPNFIYLDISNN 543

Query: 481 QISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCL 540
           QISGPLP NIGYQIPN+FAFYISNNMHINGSLPPSVCKWRHL+ LGLSNNELSGTIPSCL
Sbjct: 544 QISGPLPYNIGYQIPNMFAFYISNNMHINGSLPPSVCKWRHLMLLGLSNNELSGTIPSCL 603

Query: 541 LIPNLTVFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWIL 600
           + PNLTVF LSSNKFSG FP N F           + +L+++NL  N  +     +L   
Sbjct: 604 VTPNLTVFDLSSNKFSGFFPTNSFD---------NITTLKLVNLANNKLEGEPLVALSSC 663

Query: 601 PRLQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSN 660
             L IL L  N+  G IP    +     +   + G   N      +         ++  N
Sbjct: 664 TSLSILDLQGNQFSGSIPSWMGRSLQSLQILNLQGNSFNGTIPLSIWILPRLQILILADN 723

Query: 661 NLNDSYVHVYSMVNVDLSNNNLQGHIPRE----IVMINSLSNLNLSHNNLSGSIPVEIGR 662
            L      + +     +         P E    I  ++ +S +  S N     +     +
Sbjct: 724 KLEGEIPPIGAKFATKVEQLTYIVCNPEENEFAICYVSYISQVMKSIN-----LKYSYLQ 783

BLAST of Cucsa.054620 vs. NCBI nr
Match: gi|659068439|ref|XP_008444420.1| (PREDICTED: leucine-rich repeat receptor protein kinase EXS-like [Cucumis melo])

HSP 1 Score: 671.8 bits (1732), Expect = 1.4e-189
Identity = 395/681 (58.00%), Postives = 454/681 (66.67%), Query Frame = 1

Query: 186 SFRSKLQHLDL------SYNEFDGPIPIILRNMTSLRYLNLNG---------GLSSMVRN 245
           S+ S L++LD       S++  +G  P ++RN   L+ L+L+          G    + +
Sbjct: 294 SWLSNLRNLDTLSLGSNSFSSIEGGFPSMVRNNCHLKSLDLSFNHFVGEDVFGSYENLSS 353

Query: 246 NCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLSR- 305
            C    L  L+   +E MI FG HTIPSWLGELK+LKSLSLR NAL+GPIPSSFGNLS  
Sbjct: 354 GCKEYGLQRLY---LERMIRFGTHTIPSWLGELKNLKSLSLRENALYGPIPSSFGNLSSL 413

Query: 306 -------------IPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHL 365
                        IPISFGQLRNLN+ DVSKNSLKGVITETHFANLSQLKMVA+SSNEHL
Sbjct: 414 EDLDISYNRLRGGIPISFGQLRNLNSLDVSKNSLKGVITETHFANLSQLKMVAMSSNEHL 473

Query: 366 SFEIKHDW---------------GFGSNGFHRWLVTQKDEL------------GPIPTWL 425
           SFEIKHDW               GFGSNGF RWLVTQKDEL            GPIPTWL
Sbjct: 474 SFEIKHDWVPPFQLQYFSVRSTKGFGSNGFPRWLVTQKDELVLYLVLSNTSLSGPIPTWL 533

Query: 426 SFPNFIYLD-------------------------ISNNQ-ISGPLPDNIGYQIPNIFAFY 485
           SFPNFIYLD                         ISNN  I+G LP ++  +  ++    
Sbjct: 534 SFPNFIYLDISNNQISGPLPYNIGYQIPNMFAFYISNNMHINGSLPPSV-CKWRHLMLLG 593

Query: 486 ISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLL--IPNLTVFYLSSNKFSGV- 545
           +SNN  ++G++P S     +L    LS+N+ SG  P+     I  L +  L++NK  G  
Sbjct: 594 LSNN-ELSGTIP-SCLVTPNLTVFDLSSNKFSGFFPTNSFDNITTLKLVNLANNKLEGEP 653

Query: 546 --------------FPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQ 605
                            NQFSGSIPSWMGR LQSLQILNLQGNSF+ TIP S+WILPRLQ
Sbjct: 654 LVALSSCTSLSILDLQGNQFSGSIPSWMGRSLQSLQILNLQGNSFNGTIPLSIWILPRLQ 713

Query: 606 ILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLND 665
           ILILADNKLEGEIPPI AKFATK E+ T   ++CN +E+++ ICY+SY+ QVMKS NL  
Sbjct: 714 ILILADNKLEGEIPPIGAKFATKVEQLTY--IVCNPEENEFAICYVSYISQVMKSINLKY 773

Query: 666 SYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLD 725
           SY+ +YSMVNVDLSNNNLQGHIPR IV IN L  LNLSHNNL+GSIPVEIGRSL LESLD
Sbjct: 774 SYLQLYSMVNVDLSNNNLQGHIPRGIVTINGLFALNLSHNNLTGSIPVEIGRSLTLESLD 833

Query: 726 LSSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIPR-------------GGNPHLCGDPF 749
           LSSNQLSGSIPLNM  LNSL ALNLSNNNFSGRIPR              GNP+LCGDP 
Sbjct: 834 LSSNQLSGSIPLNMANLNSLGALNLSNNNFSGRIPREGHLSTFNNASSYEGNPYLCGDPL 893


HSP 2 Score: 70.5 bits (171), Expect = 1.5e-08
Identity = 100/385 (25.97%), Postives = 165/385 (42.86%), Query Frame = 1

Query: 72  YLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFIDGLAFRWISSLRC 131
           YL LS+T   G I  +L +    + LD +N  Q++GP  + +G    +  AF        
Sbjct: 514 YLVLSNTSLSGPIPTWL-SFPNFIYLDISNN-QISGPLPYNIGYQIPNMFAF-------- 573

Query: 132 VEYIDLSGVRVVQSGKLDVDYLIQLLNSIPS--CFSLNLSSCALQNYQLLDAPLNSSFRS 191
             YI  +               + +  S+P   C   +L    L N +L     +     
Sbjct: 574 --YISNN---------------MHINGSLPPSVCKWRHLMLLGLSNNELSGTIPSCLVTP 633

Query: 192 KLQHLDLSYNEFDGPIPI-ILRNMTSLRYLNLNG----GLSSMVRNNCHMKS-LDLLFNN 251
            L   DLS N+F G  P     N+T+L+ +NL      G   +  ++C   S LDL  N 
Sbjct: 634 NLTVFDLSSNKFSGFFPTNSFDNITTLKLVNLANNKLEGEPLVALSSCTSLSILDLQGNQ 693

Query: 252 FVEEMIGFGMHTIPSWLGE-LKSLKSLSLRGNALHGPIPSSFGNLSRIPISF-------G 311
           F          +IPSW+G  L+SL+ L+L+GN+ +G IP S   L R+ I         G
Sbjct: 694 F--------SGSIPSWMGRSLQSLQILNLQGNSFNGTIPLSIWILPRLQILILADNKLEG 753

Query: 312 QLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDWGFGSNGFHRWL 371
           ++  +     +K      I      N   +  V+  S    S  +K+ +           
Sbjct: 754 EIPPIGAKFATKVEQLTYIVCNPEENEFAICYVSYISQVMKSINLKYSY-LQLYSMVNVD 813

Query: 372 VTQKDELGPIPTWLSFPNFIY-LDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSL 431
           ++  +  G IP  +   N ++ L++S+N ++G +P  IG  +  + +  +S+N  ++GS+
Sbjct: 814 LSNNNLQGHIPRGIVTINGLFALNLSHNNLTGSIPVEIGRSL-TLESLDLSSNQ-LSGSI 860

Query: 432 PPSVCKWRHLVFLGLSNNELSGTIP 440
           P ++     L  L LSNN  SG IP
Sbjct: 874 PLNMANLNSLGALNLSNNNFSGRIP 860


HSP 3 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 357/917 (38.93%), Postives = 478/917 (52.13%), Query Frame = 1

Query: 1   WKGVGCDH-TNGGHVVKLDLRNYEYFYSSAFLS--------------------------- 60
           W GV CDH TN  HVVKLDLRNYEY YSSA LS                           
Sbjct: 61  WDGVVCDHITNASHVVKLDLRNYEYSYSSALLSKGYLYGSDGYYYPGELIVDGLEWVSSL 120

Query: 61  ----------------NGVD-SSLFE------SKYLNYLGLSANFFN-YTPIPNSFAGLL 120
                           +G+D   +F       S  L+  GL   + + Y P+ +SF  L 
Sbjct: 121 SSLEYLDLGGVLVPSKSGLDLMKVFNAMPPLLSLKLSSCGLQNTYHHIYAPLNSSF--LS 180

Query: 121 GLTYLNLSSTYFHGAIQPFLGNLTKLLVLD-FNNKGQLNGPHNFPLGELFIDGLAFRWIS 180
            + +L+LS     G I   L N+T +  L+ ++N+   + P                W+S
Sbjct: 181 KIQHLDLSYNMLDGPIPSILQNMTSMKFLNLYDNRYNSSIPS---------------WLS 240

Query: 181 SLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQL-----LDAP 240
           +L+ ++ ++L            +++L  +   + S    N   C L+N  L     L+  
Sbjct: 241 NLKNLDTLNLG-----------LNFLSSIQGGLSSMVRNN---CHLKNVDLSFNHFLNED 300

Query: 241 LNSSFRSK--------LQHLDLSYNEFDG-PIPIILRNMTSLRYLNLN-----GGLSSMV 300
           +  SF +         L+ LDL + +     IP  L  + +L++LNL      G + S  
Sbjct: 301 VFGSFENLSTGCQEYCLERLDLEHTQLGSHKIPSWLGELRNLKHLNLGNNSLYGPIPSSF 360

Query: 301 RNNCHMKSLDLLFNNFVEEMIGFGMHTIPSWLGELKSLKSLSLRGNALHGPIPSSFGNLS 360
            N  +++ L + +N    E        IP   G+L +L  L LR N L G IP SFG   
Sbjct: 361 GNLSNLEELFISYNMLTGE--------IPISFGKLSNLMILDLRENNLDGAIPKSFG--- 420

Query: 361 RIPISFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDW---- 420
                  QL NL   D SKN LKGV++E HF NL++LK + ++ N  LSFE+ H+W    
Sbjct: 421 -------QLTNLFGLDFSKNDLKGVLSEIHFVNLTRLKYLFMNENGLLSFEMNHNWVPPF 480

Query: 421 -----------GFGSNGFHRWLVTQK----------DELGPIPTWLSFPNFIYLDISNNQ 480
                      GFG   F RW+ TQK          + +GPIPTWL   N  +LD+S NQ
Sbjct: 481 QLKIFSVSSILGFGKCEFPRWIGTQKQMSYLNLFNTNVIGPIPTWLRSKNLQFLDLSYNQ 540

Query: 481 ISGPLPDNIGYQ------------------------IPNIFAFYISNNMHINGSLPPSVC 540
           I+G LP++I  Q                        + N+    +SNN  ++G++  S  
Sbjct: 541 ITGALPNSIDDQMPNLIALIISNAHISGSLPQSLCRLKNLSFLRVSNN-RLSGTIS-SCL 600

Query: 541 KWRHLVFLGLSNNELSGTIP-SCLLIPNLTVFYLSSNKFSGV---------------FPR 600
               LV L LS+N L G  P S   + NL V  L+ N+  G                   
Sbjct: 601 SVLDLVILDLSSNNLQGVFPISFQNLSNLEVMNLARNQLEGEPLMAMRSFKFLSVLDLEG 660

Query: 601 NQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQILILADNKLEGEIPPIE 660
           N+F G+IP WMGRRL +LQILNL+GN F+ TIP +LW+LPRLQILILADNKL G IPP  
Sbjct: 661 NKFCGNIPKWMGRRLHNLQILNLRGNMFNGTIPSTLWLLPRLQILILADNKLVGNIPPNV 720

Query: 661 AKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNLNDSYVHVYSMVNVDLSNNN 720
             F+      T    +CN ++D + +CY+SY+ Q +KS+ LN SY+ +YSMV +DLSNNN
Sbjct: 721 GNFSASRGPITDDDAVCNPEKDPWALCYVSYITQFIKSSPLNYSYLQLYSMVTIDLSNNN 780

Query: 721 LQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKL 755
             GHIPRE+V+IN L  LNLSHN+LSG+IPVEIGRS+ LESLDLS NQL GSIP +M  L
Sbjct: 781 FHGHIPREVVVINGLIGLNLSHNDLSGTIPVEIGRSVALESLDLSFNQLFGSIPNSMSSL 840

BLAST of Cucsa.054620 vs. NCBI nr
Match: gi|659073186|ref|XP_008467299.1| (PREDICTED: LOW QUALITY PROTEIN: receptor-like protein 12 [Cucumis melo])

HSP 1 Score: 503.4 bits (1295), Expect = 6.7e-139
Identity = 354/920 (38.48%), Postives = 476/920 (51.74%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEY---FYSSA---FLSNGVDSSLFESKYLNYLGLSANF 60
           WKGVGC+   G HV  +DLR+ +Y    Y+S+   + +N +DSSL E KYLNYL LS N 
Sbjct: 73  WKGVGCNQITG-HVTIIDLRSDDYQVDSYNSSWPLYSNNSIDSSLLELKYLNYLDLSGNH 132

Query: 61  FNYTPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLG 120
           FNYT IP+    ++ LTYLNLS+T   G + P LGNLTKL V D +   QL   +    G
Sbjct: 133 FNYTQIPSFLGSMVELTYLNLSNTVISGKVPPHLGNLTKLKVFDLSFNYQLQSNN----G 192

Query: 121 ELFIDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQ 180
           ++        WIS L  ++++ L+ +   +S       L+Q+L+ +    SL LSSC+LQ
Sbjct: 193 DV-------EWISHLSSLQFLGLTDMDFSKSLN-----LMQVLSYLTMLSSLRLSSCSLQ 252

Query: 181 N-YQLLDAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLN--------GGL- 240
           N Y  L +   SSF S++Q LDLSYN+ +G  P   +NM+SL  LNL+        GGL 
Sbjct: 253 NIYFSLSSSNYSSFLSRVQLLDLSYNQLNGSTPKAFQNMSSLNLLNLSANKFTAIEGGLY 312

Query: 241 SSMVRNNCHMKSLDL---------LFNNFVEEMI---------------GFGMHT-IPSW 300
           SS +RNNC +K ++L         LF  +  E I               G  M T IP W
Sbjct: 313 SSFIRNNCGLKEINLSWNSDLDVDLFETYENESISCFNNEYDLQVLNLRGTSMRTKIPDW 372

Query: 301 LGELKSLKSLSLRGNALHGPIPSSFGNLS--------------RIPISFG---------- 360
           LG+LK+  SL+LR + ++GP+P+S GNLS               IP SFG          
Sbjct: 373 LGKLKTW-SLNLRYSKIYGPVPASLGNLSSLEYLYLSDNALTGAIPTSFGRLLNLRELYL 432

Query: 361 --------------QLRNLNTFDVSKN-SLKGVITETHFANLSQLKMVAVSSNEHLSFEI 420
                         QL NL   D+SKN   K V+TE  FANLS+L+ + +  NEHLS ++
Sbjct: 433 SNNRLEGVSDECFIQLGNLELLDISKNLFFKAVLTEASFANLSRLETLVIDHNEHLSLDM 492

Query: 421 KHDW---------------GFGSNGFHRWLVTQKDEL----------GPIPTWLSFPNFI 480
              W               G   + F  WL  QK  +            IPTWL+  N  
Sbjct: 493 DPKWIPPFQLKSLVADSCIGCFGSEFPPWLQNQKSLIDLLLSNMSISSAIPTWLAPQNLT 552

Query: 481 YLDISNNQISGPLPDNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELS 540
            LD+S+N+++G L   I  Q+PN+ + ++SNN+ IN SL   +C+ ++L  L LSNN LS
Sbjct: 553 TLDLSHNKMTGSLITRIVDQMPNLRSLFLSNNL-INDSLLSPLCQLKNLEILDLSNNRLS 612

Query: 541 GTIPSCLLIPNLTVFYLSSNKFSGVFP---------------RNQFSGSIPSWMGRRLQS 600
           G +  CLL PNL    LSSN F G FP                N F GS+P  + +    
Sbjct: 613 GIVQGCLLTPNLFYLDLSSNNFFGTFPHSQGNLSYIQQLYLRNNNFEGSMPIVL-KNSHI 672

Query: 601 LQILNLQGNSFDDTIPFSLWI---------------------------LPRLQILILADN 660
           L  L+L+GN F   IP   W+                           L  LQIL LA N
Sbjct: 673 LDTLDLEGNKFSGNIP--TWVGNNLLNLQLLILRGNLFNGTIPSTLCKLTGLQILDLAHN 732

Query: 661 KLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMS--YLRQVMKSNNLNDSYVHV 720
           +LEG+IPP  + F     K +  G     +     +CY S  Y+ Q +KS+ LN S   +
Sbjct: 733 QLEGDIPPNLSNFDVMTRKRSKDGYYYYCEYSDAEMCYHSEKYVVQNIKSSELNYSMEQL 792

Query: 721 YS-MVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSN 749
            + +V +DLS N+L G IP E++M+  L  LNLSHNNL G+IP EIG    LESLDLS N
Sbjct: 793 KTFLVKIDLSKNHLVGSIPSEVIMLKGLFGLNLSHNNLVGTIPAEIGEMEVLESLDLSFN 852

BLAST of Cucsa.054620 vs. NCBI nr
Match: gi|659074635|ref|XP_008437710.1| (PREDICTED: receptor-like protein 12 [Cucumis melo])

HSP 1 Score: 466.8 bits (1200), Expect = 7.0e-128
Identity = 332/888 (37.39%), Postives = 447/888 (50.34%), Query Frame = 1

Query: 1    WKGVGCDHTNGGHVVKLDLR---NYEYFYSSAFLSNGVDSSLFESKYLNYLGLSANFFNY 60
            W GVGC+     HV  +DLR   N   FYS    +N +DSSL E KYLNYL LS N FNY
Sbjct: 215  WDGVGCNQITQ-HVTMIDLRSDINQVDFYSLPLSANSIDSSLLELKYLNYLDLSGNHFNY 274

Query: 61   TPIPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELF 120
            T IP+    +  LTYLNLS   F G + P LGNLTKL VLD +         N+P     
Sbjct: 275  TQIPSFLGSMEELTYLNLSFALFSGKLPPHLGNLTKLAVLDLS--------FNWPETNDD 334

Query: 121  IDGLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQ 180
            I+     WIS L  ++++ L+G+   ++       LIQ+L S P   SL L+ C LQN  
Sbjct: 335  IE-----WISHLSSLQFLSLTGIDFSKTSN-----LIQVLTSFPWLVSLRLNYCNLQNIP 394

Query: 181  LLDAPLN-SSFRSKLQHLDLSYNEFDGPIPIILRNMTSLRYLNLN--------GGLSSMV 240
                  N SSF S++Q LDLSYN+  G IP   +NMTSL++L L+        GGLSS +
Sbjct: 395  FSLVSSNYSSFLSRVQLLDLSYNQLSGSIPKAFQNMTSLKFLYLSRNKFTGIEGGLSSFI 454

Query: 241  RNNCHMKSLDLLFN----------NFVEEMIG--------------FGMHT-IP-SWLGE 300
            RNNC +K ++L  N          ++  E +G                M T IP  WLG+
Sbjct: 455  RNNCGLKVINLSMNKDFGGDVFDSSYENEFVGCSHGHDLQVLQLSDTSMKTKIPIDWLGK 514

Query: 301  LKSLKSLSLRGNALHGPIPSSFGNLS--------------RIPISFGQLRNLNTFDVSKN 360
             K+LK + L  + +HG IP++ GNLS               IP S G+L NL   D+S N
Sbjct: 515  FKNLKLVDLSYSKIHGSIPAALGNLSSIEYLDLSNNALTGEIPTSMGRLLNLKVLDISSN 574

Query: 361  SLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDW---------------GFGSNGFHR 420
            SLKGV+TE HF NLS+L  + +S NE +S ++K +W               G   + F +
Sbjct: 575  SLKGVLTEAHFVNLSKLHTLYLSYNELISLDMKPNWIPPFQLKKLDIGSCIGSYGSEFPQ 634

Query: 421  WLVTQK--DEL---------GPIPTWLSFPNFIYLDISNNQISGPLPDNIGYQIPNIFAF 480
            WL TQK  DEL           +PTW +  N   LD+S+NQI GP+ ++I  Q+P +   
Sbjct: 635  WLQTQKALDELWLSNTSLSVSCLPTWFTPQNLTTLDLSHNQIVGPIFNSIADQMPRLTHL 694

Query: 481  YISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLLIPNLTVFYLSSNKFSGVFP 540
            ++++N  +N SLP  +CK + L  L LSNN LSGT+  CL   NLT+  LSSN FSG FP
Sbjct: 695  FLNDNF-LNDSLPLVLCKLKSLFTLDLSNNRLSGTVHGCLFTLNLTLLDLSSNYFSGTFP 754

Query: 541  ---------------RNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWI---LPR 600
                            N F GS+P  + +  +SL+IL+++GN F   I    W+   L  
Sbjct: 755  DSHANPSLLYELFLRNNNFEGSMPIVL-KNAKSLKILDMEGNKFSGNI--LTWVGDNLQS 814

Query: 601  LQILILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQYVICYMSYLRQVMKSNNL 660
            LQ+L L  N   G IP                  + +++ D  +   ++    ++    +
Sbjct: 815  LQVLRLRSNLFNGTIPSSLCNLPNLQILD-----LAHNQLDGSIPSNLNNFDVMITGKGI 874

Query: 661  NDSYVHVYSMVNVDLSNNNLQ----GH------------------------IPREIVMIN 720
             +     +  + ++   N +Q     H                        IP+EI M+ 
Sbjct: 875  QEFIRFCWRRLCLNTEKNVVQFIKSNHFNYSTTQLRLMVNIDLSNNSLVGFIPKEITMLK 934

Query: 721  SLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKLNSLRALNLSNNNF 741
             L  LNLS+NNL G+IP+EIG    LESLDLS N+LSG IP ++ KLNSL AL LS+NNF
Sbjct: 935  RLIGLNLSYNNLIGTIPMEIGELESLESLDLSFNKLSGRIPQSLSKLNSLGALELSHNNF 994

BLAST of Cucsa.054620 vs. NCBI nr
Match: gi|659074635|ref|XP_008437710.1| (PREDICTED: receptor-like protein 12 [Cucumis melo])

HSP 1 Score: 43.9 bits (102), Expect = 1.5e+00
Identity = 38/129 (29.46%), Postives = 59/129 (45.74%), Query Frame = 1

Query: 628 SSNQLSGSIPLNMEKLNSLRALNLSNNNFSGRIPRGGNPHLCGDPFPNTCPNENGNHSDV 687
           SS +LSG  P + E +  +R + L +NNF G +P       C +    T   EN    ++
Sbjct: 26  SSTRLSGIFPYSHENITDIRKVYLRDNNFEGSMPIVWKNATCLEILELT---ENKLTGNI 85

Query: 688 KDKLETMWFCVIVVAGYSFGFWGVVGTLILKKSWRHAYFRFMDETKDKMSVAI--LVNMA 747
              L   W   I+ + + F         I ++  R+ YF F+DET  K+ VAI   V M 
Sbjct: 86  PTWLGAYW---ILDSCWRFS--------IEEELERYTYFNFVDETNYKVRVAIWRFVEMV 140

Query: 748 RLKQRMRGN 755
           + K+ +  N
Sbjct: 146 KAKESVFAN 140


HSP 2 Score: 38.1 bits (87), Expect = 8.0e+01
Identity = 20/58 (34.48%), Postives = 31/58 (53.45%), Query Frame = 1

Query: 580 SNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIP 638
           S+  L G  P     I  +  + L  NN  GS+P+    +  LE L+L+ N+L+G+IP
Sbjct: 26  SSTRLSGIFPYSHENITDIRKVYLRDNNFEGSMPIVWKNATCLEILELTENKLTGNIP 83


HSP 3 Score: 30.8 bits (68), Expect = 1.3e+04
Identity = 18/58 (31.03%), Postives = 30/58 (51.72%), Query Frame = 1

Query: 567 SYVHVYSMVNVDLSNNNLQGHIPREIVMINSLSNLNLSHNNLSGSIPVEIGRSLRLES 625
           S+ ++  +  V L +NN +G +P        L  L L+ N L+G+IP  +G    L+S
Sbjct: 37  SHENITDIRKVYLRDNNFEGSMPIVWKNATCLEILELTENKLTGNIPTWLGAYWILDS 94


HSP 4 Score: 453.4 bits (1165), Expect = 8.0e-124
Identity = 327/893 (36.62%), Postives = 446/893 (49.94%), Query Frame = 1

Query: 1   WKGVGCDHTNGGHVVKLDLRNYEYFYSSAFLSNGVD-SSLFESKYLNYLGLSANFFNYTP 60
           WKGV CD  + GHVVKLDLR         F +  V  S L E KYL YL LS N     P
Sbjct: 64  WKGVSCDDQSAGHVVKLDLRAKRLRTEVHFQATEVSLSCLLELKYLKYLDLSGNNLGDGP 123

Query: 61  IPNSFAGLLGLTYLNLSSTYFHGAIQPFLGNLTKLLVLDFNNKGQLNGPHNFPLGELFID 120
           IP  F  +  LTYLNLSST F G +   LGNLT L +LD +N+         PL  L +D
Sbjct: 124 IPKFFGSMKQLTYLNLSSTQFSGTVPHHLGNLTSLQILDLSNQ---------PL--LIVD 183

Query: 121 GLAFRWISSLRCVEYIDLSGVRVVQSGKLDVDYLIQLLNSIPSCFSLNLSSCALQNYQLL 180
            L   W+S L  + ++D+S + + ++  L     +Q+L+ +PS   L LS+C L   Q  
Sbjct: 184 DLL--WLSRLSSLHHLDMSDLYLGETLNL-----MQVLSMLPSLLWLRLSNCGLDETQFP 243

Query: 181 DAPLNSSFRSKLQHLDLSYNEFDGPIPIILRNMTSLR----------------------- 240
               N++F   LQ LDL+ N   GPIP  LRNMTSLR                       
Sbjct: 244 GGGTNATFLHNLQSLDLANNGLLGPIPDALRNMTSLRRLDLSFNILQSSIPAWLGNLENL 303

Query: 241 -YLN--------LNGGLSSMVRNNCHMKSLDLLFNNFVEEMIG----------FGMHT-- 300
            +LN        + GGL S++RN CH+KSLDL  N F  E++G          + + T  
Sbjct: 304 VHLNLSHNIFNSIEGGLLSILRNACHLKSLDLSLNQFQGEILGTSGTLSGCTEYDLDTLR 363

Query: 301 ---------IPSWLGELKSLKSLSLRGNALHGPIPSSFGNLS--------------RIPI 360
                    +P WLG+L++LK L  + N+  GPIP S GNLS               +P 
Sbjct: 364 LVNNGASGRLPDWLGKLRNLKYLDFQMNSFSGPIPFSLGNLSTLRELYLSHNQLNGTVPE 423

Query: 361 SFGQLRNLNTFDVSKNSLKGVITETHFANLSQLKMVAVSSNEHLSFEIKHDW-------- 420
           S GQL NL   D+S N++ G+++E H ANLS+L+++  + N HL+  +K +W        
Sbjct: 424 SLGQLSNLTVLDLSSNNIDGIVSEVHIANLSKLEILNFALN-HLTIRVKSNWEPPFQLKH 483

Query: 421 ------GFGSNGFHRWLVTQK---------DELGPIPTWLSFPNFIYLDISNNQISGPLP 480
                  FG+  F +WL+TQK            G IP  L   +  YLD+S N+I+G LP
Sbjct: 484 VRMESCKFGTQ-FPQWLLTQKTITLDLSNASISGTIPKSLHGLHLTYLDLSYNEITGSLP 543

Query: 481 DNIGYQIPNIFAFYISNNMHINGSLPPSVCKWRHLVFLGLSNNELSGTIPSCLL-IPNLT 540
                 +P + + ++ +N HI GS+P S+C       L LS N LSG IP CL  + NL 
Sbjct: 544 QKFSDGMPRLDSLFLGSN-HIKGSVPTSLCNIETSGALDLSKNRLSGEIPGCLKNLQNLY 603

Query: 541 VFYLSSNKFSGVFPRNQFSGSIPSWMGRRLQSLQILNLQGNSFDDTIPFSLWILPRLQIL 600
              LSSN  SGV         IPS +G  L+ L  LNL  NS    +P +L     L+ L
Sbjct: 604 AVDLSSNNLSGV---------IPSSVG-YLRHLVWLNLNNNSLHGELPSALRNCSYLKHL 663

Query: 601 ILADNKLEGEIPPIEAKFATKFEKSTVSGVMCNSKEDQY--VICYMSYLRQVMKSNNLND 660
            L +N+L G IP   A     FE   +  +  N+        +C +S L+ V  S N  +
Sbjct: 664 DLGNNRLSGHIP---AWIGDSFEFLIILRLRSNTFNGSIPSQLCQLSPLQLVQYSTNQPE 723

Query: 661 SYVHVYSM---------------------------VNVDLSNNNLQGHIPREIVMINSLS 720
           S    Y +                           V++DLS+NNL G IP E+  ++ L 
Sbjct: 724 SDQQGYDIDAEPNNKNIWQVLKGREYESARISSLGVSMDLSSNNLVGFIPDELTSLSGLL 783

Query: 721 NLNLSHNNLSGSIPVEIGRSLRLESLDLSSNQLSGSIPLNMEKL--NSLRALNLSNNNFS 745
            LNLSHN+L GSIP +IG    LES D+S+NQLSG+IP++M  L   +L  LNLS NN S
Sbjct: 784 GLNLSHNHLEGSIPQKIGELKSLESFDISNNQLSGTIPMSMWSLAMATLSHLNLSYNNLS 843

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GSO1_ARATH2.8e-5229.58LRR receptor-like serine/threonine-protein kinase GSO1 OS=Arabidopsis thaliana G... [more]
GSO2_ARATH3.1e-5130.00LRR receptor-like serine/threonine-protein kinase GSO2 OS=Arabidopsis thaliana G... [more]
Y1341_ARATH9.3e-4830.33Probable LRR receptor-like serine/threonine-protein kinase At1g34110 OS=Arabidop... [more]
FLS2_ARATH6.7e-4629.30LRR receptor-like serine/threonine-protein kinase FLS2 OS=Arabidopsis thaliana G... [more]
Y1571_ARATH2.5e-4529.32Probable leucine-rich repeat receptor-like protein kinase At1g35710 OS=Arabidops... [more]
Match NameE-valueIdentityDescription
M5WZX0_PRUPE5.5e-12436.62Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001022mg PE=4 SV=1[more]
M5WZX0_PRUPE4.2e-1526.05Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001022mg PE=4 SV=1[more]
A0A059BIT6_EUCGR9.2e-10334.30Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G03161 PE=4 SV=1[more]
A0A0A0KL22_CUCSA2.0e-9741.45Serine/threonine-protein kinase bri1 OS=Cucumis sativus GN=Csa_5G167740 PE=4 SV=... [more]
A0A0A0KL22_CUCSA1.2e-5733.23Serine/threonine-protein kinase bri1 OS=Cucumis sativus GN=Csa_5G167740 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT2G34930.14.9e-5528.49 disease resistance family protein / LRR family protein[more]
AT4G20140.11.6e-5329.58 Leucine-rich repeat transmembrane protein kinase[more]
AT5G44700.11.7e-5230.00 Leucine-rich repeat transmembrane protein kinase[more]
AT3G28890.13.9e-5231.03 receptor like protein 43[more]
AT1G74170.11.1e-5128.68 receptor like protein 13[more]
Match NameE-valueIdentityDescription
gi|659068439|ref|XP_008444420.1|3.4e-22357.93PREDICTED: leucine-rich repeat receptor protein kinase EXS-like [Cucumis melo][more]
gi|659068439|ref|XP_008444420.1|1.4e-18958.00PREDICTED: leucine-rich repeat receptor protein kinase EXS-like [Cucumis melo][more]
gi|659073186|ref|XP_008467299.1|6.7e-13938.48PREDICTED: LOW QUALITY PROTEIN: receptor-like protein 12 [Cucumis melo][more]
gi|659074635|ref|XP_008437710.1|7.0e-12837.39PREDICTED: receptor-like protein 12 [Cucumis melo][more]
gi|659074635|ref|XP_008437710.1|1.5e+0029.46PREDICTED: receptor-like protein 12 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001611Leu-rich_rpt
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.054620.1Cucsa.054620.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 265..287
score: 0.77coord: 621..642
score:
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 189..203
score:
NoneNo IPR availablePRINTSPR00019LEURICHRPTcoord: 622..635
score: 3.1E-5coord: 643..656
score: 3.
NoneNo IPR availablePANTHERPTHR27004FAMILY NOT NAMEDcoord: 1..100
score: 2.7E-173coord: 149..746
score: 2.7E
NoneNo IPR availableunknownSSF52047RNI-likecoord: 570..663
score: 2.35E-43coord: 254..314
score: 2.35E-43coord: 361..525
score: 2.35

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.054620Csa1G435730Cucumber (Chinese Long) v2cgycuB039
Cucsa.054620MELO3C002747Melon (DHL92) v3.5.1cgymeB038
Cucsa.054620CSPI01G21040Wild cucumber (PI 183967)cgycpiB040
Cucsa.054620MELO3C002747.2Melon (DHL92) v3.6.1cgymedB038
The following gene(s) are paralogous to this gene:

None