Cucsa.047700 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.047700 vs. Swiss-Prot
Match: MNS3_ARATH (Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 OS=Arabidopsis thaliana GN=MNS3 PE=1 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 7.3e-264
Identity = 454/634 (71.61%), Postives = 525/634 (82.81%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYSVKD+HYDNAKFR RS +KV +Q+LLT S KR+Y SCSTGKFL+L+L FG+A 
Sbjct: 1   MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVAC 60

Query: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDE---KGSN 120
           L+L S +P          S  +EK ++   GG+    L RK PRLPP+L PDE   +GS+
Sbjct: 61  LMLMSKSP--------NESGLNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGSS 120

Query: 121 TSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGA 180
           T+     N   S+  W +RQQ VK+AF HAWSGY++YAMGYDELMP+SQ G DGLGGLGA
Sbjct: 121 TNGSTISN---SDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLGA 180

Query: 181 TVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRA 240
           TVVDALDTAMIMG  +IVSEAGSW+E +L++RI +KGQVNLFETTIR++GGLLSAYHL  
Sbjct: 181 TVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSG 240

Query: 241 GEYGKT-FGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSS 300
           GE G     + GPKP +YL IAK+LADRLLSAFTSSPT +P CDV+L +S+AH APGG+S
Sbjct: 241 GEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGAS 300

Query: 301 STSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGET 360
           ST+EV+++QLEFNYLS++SGDPKYS  AMKVL H+K+LPK EGLVPIYISP TG F GE 
Sbjct: 301 STAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGEN 360

Query: 361 IRLGSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVG 420
           IRLGSRGDSYYEYL+KVWLQ+    NSNFTYLHDMY EAMKGV+HLLV+ SIP GLVFVG
Sbjct: 361 IRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFVG 420

Query: 421 ELPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAK 480
           ELPYGS    SPKMDHLVCFL GTLALGATKG+TKE+A+ ++LL+F+DLENL LAEDLAK
Sbjct: 421 ELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLAK 480

Query: 481 TCFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVE 540
           TCFEMYEVT+TGLAPEIAYFHT++Y+E GLDGGNK S Y +DIIIKP DRHNL+RPETVE
Sbjct: 481 TCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETVE 540

Query: 541 SLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGE 600
           SLFVLYRIT D KYR  GW+IFEAFE++TKV SGGYTSLDDVT +PP RRDKMETFFLGE
Sbjct: 541 SLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGE 600

Query: 601 TLKYLYLLFGDSSTIPLDKYVFNTEAHPLPMDGN 631
           TLKYLYLLFGD S IPLDK+VFNTEAHPLP+  N
Sbjct: 601 TLKYLYLLFGDDSVIPLDKFVFNTEAHPLPIRRN 623

BLAST of Cucsa.047700 vs. Swiss-Prot
Match: MNS1_CANAX (Mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Candida albicans GN=MNS1 PE=3 SV=2)

HSP 1 Score: 399.8 bits (1026), Expect = 5.5e-110
Identity = 243/509 (47.74%), Postives = 313/509 (61.49%), Query Frame = 1

Query: 130 ESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTD-GLGGLGATVVDALDTAMI 189
           +S W   Q +V+ A + +W  Y++Y  GYD   P+ Q G + G   LG  +VD+LDT MI
Sbjct: 32  QSKWERAQSEVRQAILDSWHTYEKYGWGYDVYHPIKQEGENMGPKPLGWMIVDSLDTLMI 91

Query: 190 MGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEYGKTFGNEG 249
           M   + VS A  WI KN +D   +   VN FETTIR++GGLLSAYH         F N+ 
Sbjct: 92  MDCPEEVSRARDWI-KNDLDYTFDYN-VNTFETTIRMLGGLLSAYH---------FSNDD 151

Query: 250 PKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSA--HAAPGGSSSTSEVSTLQL 309
               VYL+ A  LA+ L  A+  SP+ IP   V LK      +    G+SST+E +T+QL
Sbjct: 152 ----VYLDKAVQLANALHGAY-DSPSGIPYSSVNLKSGKGIKNHVDNGASSTAEAATVQL 211

Query: 310 EFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLGSRGDSY 369
           E  YLS ++G+  +   A KV++ ++S    +GLVPIY++P TGK+ G  IRLGSRGDSY
Sbjct: 212 EMKYLSKLTGEILWWNLAEKVMQVLESNKPQDGLVPIYVNPDTGKYQGHLIRLGSRGDSY 271

Query: 370 YEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTI 429
           YEYLLK +LQ     N       DMY E+++GVK  LV  S P+GL F+GEL  G    +
Sbjct: 272 YEYLLKQYLQT----NKQELVYWDMYRESVEGVKKHLVSDSYPSGLTFIGELDNGIGGKL 331

Query: 430 SPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMY-EVT 489
           S KMDHLVCF  G LALGAT G+T  EA S    N +   +  L E+L  TC++MY +V+
Sbjct: 332 STKMDHLVCFYGGLLALGATGGLTLNEAQSLKSWNEEREADFKLGEELTYTCYKMYHDVS 391

Query: 490 STGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFVLYRIT 549
            TGL+PEI  F+ +            KSK   D IIKPLDRHNL RPETVESLF LYR+T
Sbjct: 392 PTGLSPEIVVFNED----------TSKSK---DFIIKPLDRHNLQRPETVESLFYLYRLT 451

Query: 550 GDPKYRAWGWEIFEAFERHTKV--SSG--GYTSLDDVTTIP----PRRRDKMETFFLGET 609
           GD KYR  G+EIF+ F ++TKV  S G   ++SL DVT+      P+ +D  E+F+  ET
Sbjct: 452 GDVKYREMGYEIFQNFIKYTKVVNSEGEVSFSSLSDVTSFDSNGLPKFKDNTESFWWAET 507

Query: 610 LKYLYLLFGDSSTIPLDKYVFNTEAHPLP 627
           LKYLYLLF D++ IPL  YVFNTEAHP P
Sbjct: 512 LKYLYLLFDDTNKIPLTDYVFNTEAHPFP 507

BLAST of Cucsa.047700 vs. Swiss-Prot
Match: MA1B1_HUMAN (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Homo sapiens GN=MAN1B1 PE=1 SV=2)

HSP 1 Score: 393.3 bits (1009), Expect = 5.1e-108
Identity = 238/532 (44.74%), Postives = 324/532 (60.90%), Query Frame = 1

Query: 101 KAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDE 160
           + P  PP LPP  +   T  H++            RQ+ V D F+HAW GY+++A G+DE
Sbjct: 227 EVPTKPP-LPP-ARTQGTPVHLN-----------YRQKGVIDVFLHAWKGYRKFAWGHDE 286

Query: 161 LMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFE 220
           L P+S+  ++  G LG T++DALDT  I+G      EA  W+ K L    ++   VNLFE
Sbjct: 287 LKPVSRSFSEWFG-LGLTLIDALDTMWILGLRKEFEEARKWVSKKL--HFEKDVDVNLFE 346

Query: 221 TTIRIVGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCD 280
           +TIRI+GGLLSAYHL                +++L  A++  +RL+ AF + P+ IP  D
Sbjct: 347 STIRILGGLLSAYHLSGD-------------SLFLRKAEDFGNRLMPAFRT-PSKIPYSD 406

Query: 281 VVLKDSSAHAAPGGSSST-SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLP-KVE 340
           V +    AH     S ST +EV+++QLEF  LS ++GD K+  A  KV +H+  L  K +
Sbjct: 407 VNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKD 466

Query: 341 GLVPIYISPHTGKFSGETI-RLGSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMK 400
           GLVP++I+ H+G F+   +  LG+R DSYYEYLLK W+Q    G    T L + Y EA++
Sbjct: 467 GLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQ----GGKQETQLLEDYVEAIE 526

Query: 401 GVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSK 460
           GV+  L+R S P+ L FVGEL +G     S KMDHLVCFL GTLALG   G+        
Sbjct: 527 GVRTHLLRHSEPSKLTFVGELAHGR---FSAKMDHLVCFLPGTLALGVYHGLPAS----- 586

Query: 461 SLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYID 520
                    ++ LA++L +TC++M     TGL+PEI +F+   Y + G            
Sbjct: 587 ---------HMELAQELMETCYQMNRQMETGLSPEIVHFNL--YPQPGRR---------- 646

Query: 521 DIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDD 580
           D+ +KP DRHNL+RPETVESLF LYR+TGD KY+ WGWEI ++F R T+V SGGY+S+++
Sbjct: 647 DVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINN 695

Query: 581 V-TTIPPRRRDKMETFFLGETLKYLYLLFGDS-STIPLDKYVFNTEAHPLPM 628
           V     P  RDKME+FFLGETLKYL+LLF D  + + LD YVFNTEAHPLP+
Sbjct: 707 VQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPI 695

BLAST of Cucsa.047700 vs. Swiss-Prot
Match: MA1B1_RAT (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Rattus norvegicus GN=Man1b1 PE=2 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 1.1e-105
Identity = 236/542 (43.54%), Postives = 331/542 (61.07%), Query Frame = 1

Query: 99  WRKAPRLPPQL--PPDEKGSNTSYHIDPNTLISESLWIS------RQQKVKDAFIHAWSG 158
           WR A   P Q   PP ++   +   I P  L S  +W        RQ+ V +AF+HAW G
Sbjct: 166 WRGAVIEPEQATEPPSKRAEAS---IKPLFLASR-IWKEPAPPNERQKGVIEAFLHAWKG 225

Query: 159 YKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKNLMDRI 218
           Y+++A G+DEL P+S+  ++  G LG T++DALDT  I+G      EA  W+ +NL    
Sbjct: 226 YQKFAWGHDELKPVSKTFSEWFG-LGLTLIDALDTMWILGLKQEFKEARKWVSENL--DF 285

Query: 219 KEKGQVNLFETTIRIVGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLSAFT 278
           ++   VNLFE+TIRI+GGLLSAYHL                +++L  A++  +RL+ AFT
Sbjct: 286 QKNVDVNLFESTIRILGGLLSAYHLSGD-------------SLFLSKAEDFGNRLMPAFT 345

Query: 279 SSPTAIPLCDVVLKDSSAHAAPGGSSST-SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLE 338
           + P+ IP  DV +    AH+    S ST +EV+++QLEF  LS ++G  K+  A  +V +
Sbjct: 346 T-PSKIPYSDVNIGTGFAHSPQWTSDSTVAEVTSIQLEFRELSRLTGIKKFQEAVEEVTK 405

Query: 339 HMKSLP-KVEGLVPIYISPHTGKFSGETI-RLGSRGDSYYEYLLKVWLQKRTSGNSNFTY 398
           H+ SL  K +GLVP++I+ ++G F+   +  LG+R DSYYEYLLK W+Q    G    T 
Sbjct: 406 HIHSLSGKKDGLVPMFINTNSGLFTHPGVFTLGARADSYYEYLLKQWIQ----GGKKETQ 465

Query: 399 LHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDHLVCFLSGTLALGATK 458
           L + Y  A++G+K  L+R+S P  L FVGEL +G     S KMDHLVCFL GTLALG   
Sbjct: 466 LLEDYVRAIEGIKAHLLRQSQPRKLTFVGELAHGR---FSAKMDHLVCFLPGTLALGVHH 525

Query: 459 GITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGLD 518
           G+  +              +++LA  L +TC++M +   TGL+PEIA+F+    ++    
Sbjct: 526 GLPAD--------------HMDLARALMETCYQMNQQMETGLSPEIAHFNMYPRADH--- 585

Query: 519 GGNKKSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTKV 578
                     D+ +KP DRHNL+RPETVESLF LYR+T D KY+ WGWEI ++F ++T+V
Sbjct: 586 ---------KDVEVKPADRHNLLRPETVESLFYLYRVTKDRKYQDWGWEILQSFNKYTRV 645

Query: 579 SSGGYTSLDDV-TTIPPRRRDKMETFFLGETLKYLYLLFGDS-STIPLDKYVFNTEAHPL 628
            SGGY+S+++V  +  P  RDKME+FF+GETLKYLYLLF D    + LD  VFNTEAHPL
Sbjct: 646 PSGGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLFSDDLELLGLDTCVFNTEAHPL 653

BLAST of Cucsa.047700 vs. Swiss-Prot
Match: MA1B1_MOUSE (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Mus musculus GN=Man1b1 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.2e-104
Identity = 222/497 (44.67%), Postives = 314/497 (63.18%), Query Frame = 1

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIV 195
           RQ+ V +AF+HAW GY+++A G+DEL P+S+  ++  G LG T++DALDT  I+G     
Sbjct: 208 RQKGVIEAFLHAWKGYQKFAWGHDELKPVSKTFSEWFG-LGLTLIDALDTMWILGLKQEF 267

Query: 196 SEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEYGKTFGNEGPKPAVYL 255
            +A  W+ +NL    ++   VNLFE+TIRI+GGLLS YHL                +++L
Sbjct: 268 KQARKWVSENL--DFQKNVDVNLFESTIRILGGLLSTYHLSGD-------------SLFL 327

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSST-SEVSTLQLEFNYLSTV 315
             A++   RL+ AFT+ P+ IP  DV +    AH+    S ST +EV+++QLEF  LS +
Sbjct: 328 TKAEDFGKRLMPAFTT-PSKIPYSDVNIGTGFAHSPQWTSDSTVAEVTSIQLEFRELSRL 387

Query: 316 SGDPKYSIAAMKVLEHMKSLP-KVEGLVPIYISPHTGKFSGETI-RLGSRGDSYYEYLLK 375
           +G  K+  A  +V +H+ SL  K +GLVP++I+ ++G F+   +  LG+R DSYYEYLLK
Sbjct: 388 TGIKKFQEAVEEVTKHIHSLSGKKDGLVPMFINTNSGLFTHPGVFTLGARADSYYEYLLK 447

Query: 376 VWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDH 435
            W+Q    G    T L + Y +A++G+K  L+R+S P  L FVGEL +G     S KMDH
Sbjct: 448 QWIQ----GGKKETQLLEDYVKAIEGIKAHLLRQSQPRKLTFVGELAHGR---FSAKMDH 507

Query: 436 LVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPE 495
           LVCFL GTLALG   G+  +              +++LA  L +TC++M +   TGL+PE
Sbjct: 508 LVCFLPGTLALGVHHGLPAD--------------HMDLARALMETCYQMNQQMETGLSPE 567

Query: 496 IAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRA 555
           IA+F+    ++              D+ +KP DRHNL+RPETVESLF LYR+T D KY+ 
Sbjct: 568 IAHFNMYPRADH------------KDVEVKPADRHNLLRPETVESLFYLYRVTRDRKYQD 627

Query: 556 WGWEIFEAFERHTKVSSGGYTSLDDV-TTIPPRRRDKMETFFLGETLKYLYLLFGDS-ST 615
           WGWEI ++F ++T+V SGGY+S+++V  +  P  RDKME+FF+GETLKYLYLLF D    
Sbjct: 628 WGWEILQSFNKYTRVPSGGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLFSDDLEL 654

Query: 616 IPLDKYVFNTEAHPLPM 628
           + LD  VFNTEAHPLP+
Sbjct: 688 LSLDSCVFNTEAHPLPI 654

BLAST of Cucsa.047700 vs. TrEMBL
Match: A0A0A0KLY9_CUCSA (alpha-1,2-Mannosidase OS=Cucumis sativus GN=Csa_6G523340 PE=3 SV=1)

HSP 1 Score: 1274.6 bits (3297), Expect = 0.0e+00
Identity = 634/634 (100.00%), Postives = 634/634 (100.00%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY
Sbjct: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
           HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE
Sbjct: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600
           LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 635
           LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 634

BLAST of Cucsa.047700 vs. TrEMBL
Match: B9H405_POPTR (alpha-1,2-Mannosidase OS=Populus trichocarpa GN=POPTR_0004s13920g PE=3 SV=2)

HSP 1 Score: 954.9 bits (2467), Expect = 4.9e-275
Identity = 472/632 (74.68%), Postives = 537/632 (84.97%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYS+KDVHYDNAKFRQRS+VKV+TQ+LLT + K D LSCSTGKFL LL+IFGLA 
Sbjct: 1   MSKSLPYSMKDVHYDNAKFRQRSFVKVITQSLLTSNAKHDCLSCSTGKFLALLMIFGLAC 60

Query: 61  LLLT-SATPVHVPNKLAK-VSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNT 120
           L+ T  +TP  V    A+ +  N E + I ++GG  F+  WRK PRLPPQLPPDEKGS  
Sbjct: 61  LMFTHKSTPHSVSQGQAEGIKVNKEVKGISDSGG-RFQRFWRKPPRLPPQLPPDEKGSIG 120

Query: 121 SYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGAT 180
           +   +   L ++S W+ RQQ+V+ AFIHAWSGYK YAMGYDELMPLSQ G DGLGGLGAT
Sbjct: 121 NLPHELQKLNTDSKWVDRQQRVQKAFIHAWSGYKMYAMGYDELMPLSQRGVDGLGGLGAT 180

Query: 181 VVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAG 240
           VVDALDTA+IMG  ++VSEAG WIE +L DRI +KGQVNLFETTIR++GGLLSAYH   G
Sbjct: 181 VVDALDTALIMGVDEVVSEAGLWIETHLSDRISKKGQVNLFETTIRVLGGLLSAYHFSGG 240

Query: 241 EYGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSST 300
           E G    ++GPKP VYLE A+NLADRLL AFTSSPT+IP  DVVL D SAH AP G SST
Sbjct: 241 EQGTNLTHKGPKPTVYLETARNLADRLLLAFTSSPTSIPFSDVVLHDPSAHRAPDGLSST 300

Query: 301 SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIR 360
           +EVST+QLEFNYLSTVSGDP+YSI AMKVLEH+K LPKVEGLVPIYISP +G+FSGE IR
Sbjct: 301 AEVSTVQLEFNYLSTVSGDPRYSIEAMKVLEHIKDLPKVEGLVPIYISPDSGEFSGENIR 360

Query: 361 LGSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGEL 420
           LGSRGDSYYEYL+KVWLQ+  + +SNFTYL+DMYEEAMKGV+HLLV+KSIPNGLVFVGEL
Sbjct: 361 LGSRGDSYYEYLIKVWLQQGRNRDSNFTYLYDMYEEAMKGVRHLLVQKSIPNGLVFVGEL 420

Query: 421 PYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTC 480
           PYG   + SPKMDHLVCFL GTLALGATKG+TKE+AM ++LL F+DLENL LAEDLAKTC
Sbjct: 421 PYGPKGSFSPKMDHLVCFLPGTLALGATKGLTKEKAMKENLLKFEDLENLKLAEDLAKTC 480

Query: 481 FEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESL 540
           FEMY VTSTGLAPEIAYFHTEEYSE GLDGGNK SK+++DIIIK  DRHNL+RPETVESL
Sbjct: 481 FEMYSVTSTGLAPEIAYFHTEEYSEHGLDGGNKNSKFVNDIIIKHADRHNLLRPETVESL 540

Query: 541 FVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETL 600
           F+LYRIT DPKY+ WGW+IFEAFE++TKV SGGY+SL+DVT +PPRRRDKMETFFLGETL
Sbjct: 541 FILYRITEDPKYQEWGWQIFEAFEKYTKVDSGGYSSLEDVTVLPPRRRDKMETFFLGETL 600

Query: 601 KYLYLLFGDSSTIPLDKYVFNTEAHPLPMDGN 631
           KY YLLFGD S IPLDKYVFNTEAHPLP+ G+
Sbjct: 601 KYFYLLFGDRSVIPLDKYVFNTEAHPLPIKGS 631

BLAST of Cucsa.047700 vs. TrEMBL
Match: F6HLR4_VITVI (alpha-1,2-Mannosidase OS=Vitis vinifera GN=VIT_10s0003g03280 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 1.3e-272
Identity = 471/634 (74.29%), Postives = 533/634 (84.07%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYS+KDVHYDNAKFR RS  K++TQ +LT + KRD +SCSTGKFLVLL+I GLAY
Sbjct: 1   MSKSLPYSMKDVHYDNAKFRHRSCFKMITQTMLTSNLKRDCISCSTGKFLVLLMIGGLAY 60

Query: 61  LLLTSATPVHVP-NKLAK--VSENSEKQEIKN--NGGINFRNLWRKAPRLPPQLPPDEKG 120
           L+LT+ +  H   N LAK  + +   K+  K+  +GG  FR  WRK PRLPP+L PDEKG
Sbjct: 61  LMLTNTSSGHSDSNGLAKGVIFKGGIKEGDKSIMDGGNRFRRFWRKPPRLPPRLSPDEKG 120

Query: 121 SNTSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGL 180
           SN S+  DP  L ++S+WI+RQQ VK AFIHAWSGYK+YAMG+DELMPLS  G DGLGGL
Sbjct: 121 SNNSFIHDPVKLNADSIWIARQQSVKKAFIHAWSGYKKYAMGFDELMPLSHRGVDGLGGL 180

Query: 181 GATVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHL 240
           GAT+VDALDTAMIMGA ++V+EAGSWIE +L  RIKEKGQVNLFETTIR++GGLLSAYHL
Sbjct: 181 GATIVDALDTAMIMGADEVVTEAGSWIETHLSHRIKEKGQVNLFETTIRVLGGLLSAYHL 240

Query: 241 RAGEYGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGS 300
             GE G    + GPK  VYLE AK LAD LLSAFTSSPT IP  DVVL+D SAH AP G 
Sbjct: 241 SGGEQGMNSTHMGPKSIVYLETAKQLADLLLSAFTSSPTPIPFSDVVLRDHSAHPAPDGQ 300

Query: 301 SSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGE 360
           SSTSEVSTLQLEFNYLSTVSGDPKY + AMKVLEH+K+LPKVEGLVPIYISPH+G+F+GE
Sbjct: 301 SSTSEVSTLQLEFNYLSTVSGDPKYGVEAMKVLEHLKTLPKVEGLVPIYISPHSGEFNGE 360

Query: 361 TIRLGSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFV 420
            IRLGSRGDSYYEYL+KVWLQ+    + NF YL+DMYEEA KG++HLLV+KSIP GLVFV
Sbjct: 361 NIRLGSRGDSYYEYLIKVWLQQGAERDGNFKYLYDMYEEATKGIRHLLVQKSIPKGLVFV 420

Query: 421 GELPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLA 480
           GELP GS    SPKMDHLVCFL GTLALGATKGITKE+AM  +LL F+DLENL LAEDLA
Sbjct: 421 GELPSGSKGAFSPKMDHLVCFLPGTLALGATKGITKEKAMKDNLLTFEDLENLKLAEDLA 480

Query: 481 KTCFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETV 540
           KTCFEMY VTSTGLAPEIAYFHTE+Y EQGLDGGNK S+Y++DIIIK  DRHNL+RPETV
Sbjct: 481 KTCFEMYSVTSTGLAPEIAYFHTEDYFEQGLDGGNKSSEYMNDIIIKYADRHNLLRPETV 540

Query: 541 ESLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLG 600
           ESLFVLYRIT +PKYR WGW+IFEAFE+HTKV SGGY+SLDDVT +PP+RRDKMETFFLG
Sbjct: 541 ESLFVLYRITENPKYREWGWQIFEAFEKHTKVDSGGYSSLDDVTRLPPQRRDKMETFFLG 600

Query: 601 ETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPMDG 630
           ETLKYLYLLF DS+ IPLDK+VFNTEAHP P+ G
Sbjct: 601 ETLKYLYLLFADSTVIPLDKFVFNTEAHPFPIKG 634

BLAST of Cucsa.047700 vs. TrEMBL
Match: I1MF37_SOYBN (alpha-1,2-Mannosidase OS=Glycine max GN=GLYMA_15G094500 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 1.7e-272
Identity = 464/631 (73.53%), Postives = 535/631 (84.79%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYS KDV YDNAKFR RS+ KV TQNLLT + +RD  SCSTGKFL L+LIFG+AY
Sbjct: 1   MSKSLPYSTKDVDYDNAKFRPRSFFKVATQNLLTSNLRRDCGSCSTGKFLFLILIFGVAY 60

Query: 61  LLL--TSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGS-N 120
           L+L  TS+  V V +  A V+ NS       NG    +  WR+ PRLPP+L PDEKGS N
Sbjct: 61  LMLAHTSSGGV-VSDDEAIVNRNSYANSSIVNGAGKIKRFWRRPPRLPPRLSPDEKGSGN 120

Query: 121 TSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGA 180
            + H+  N  +  S+WI+RQ+KVK+AF+HAWSGYK++AMGYDELMPLSQHG DGLGGLGA
Sbjct: 121 GNDHVSGNPDVVRSMWIARQKKVKEAFVHAWSGYKKFAMGYDELMPLSQHGIDGLGGLGA 180

Query: 181 TVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRA 240
           TVVDALDTAMIMG  ++V+EAGSW+E++L +RI +KGQVNLFETTIR++GGLLSAYHL  
Sbjct: 181 TVVDALDTAMIMGLDEVVAEAGSWVEEHLSERISKKGQVNLFETTIRVLGGLLSAYHLSG 240

Query: 241 GEYGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSS 300
           GE G    + GPKPAVYLE AK+LADRLLSAFT+SPTAIP  DV+L + SAH APGG SS
Sbjct: 241 GEKGTNLTHAGPKPAVYLETAKDLADRLLSAFTASPTAIPFSDVILHEKSAHPAPGGLSS 300

Query: 301 TSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETI 360
           TSEVSTLQLEFNYLS +SGD KYS+ AMKV+EHMK+LPK+EGLVPIYISPH+G+FSGE I
Sbjct: 301 TSEVSTLQLEFNYLSQISGDQKYSLEAMKVMEHMKTLPKIEGLVPIYISPHSGEFSGENI 360

Query: 361 RLGSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGE 420
           RLGSRGDSYYEYL+KVWLQ   S NSN +YL++MY EAM GV+HLLVRKSIPNGLVFVGE
Sbjct: 361 RLGSRGDSYYEYLIKVWLQSGASSNSNTSYLYEMYNEAMNGVRHLLVRKSIPNGLVFVGE 420

Query: 421 LPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKT 480
           LPYGS+S  SPKMDHLVCFL GTLA+GATKG+TK++AM  ++LNF+DLENL LAEDL KT
Sbjct: 421 LPYGSNSNFSPKMDHLVCFLPGTLAIGATKGLTKKQAMKNNMLNFEDLENLKLAEDLTKT 480

Query: 481 CFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVES 540
           CFEMY VTSTGLAPEIAYFHTEE+SEQG DGGNK S++++DIIIKP DRHNL+RPETVES
Sbjct: 481 CFEMYAVTSTGLAPEIAYFHTEEFSEQGHDGGNKSSEFVNDIIIKPADRHNLLRPETVES 540

Query: 541 LFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGET 600
           LFVLYRIT DPKYR WGW+IFEAFE+HTKV +GGY SLDDVT +PP RRDKMETFFLGET
Sbjct: 541 LFVLYRITEDPKYREWGWQIFEAFEKHTKVDTGGYCSLDDVTIVPPHRRDKMETFFLGET 600

Query: 601 LKYLYLLFGDSSTIPLDKYVFNTEAHPLPMD 629
           LKY YLLF DSS IPLDK+VFNTEAHP+P++
Sbjct: 601 LKYFYLLFADSSLIPLDKFVFNTEAHPIPIN 630

BLAST of Cucsa.047700 vs. TrEMBL
Match: A0A067L0E5_JATCU (alpha-1,2-Mannosidase OS=Jatropha curcas GN=JCGZ_04608 PE=3 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 5.1e-272
Identity = 470/630 (74.60%), Postives = 535/630 (84.92%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYS+KDVHYDNAKFRQRS VKV++Q+L+T + KR+ +SCSTGKFL+LL+IFGLAY
Sbjct: 1   MSKSLPYSMKDVHYDNAKFRQRSLVKVISQSLITTNLKRECISCSTGKFLLLLMIFGLAY 60

Query: 61  LLLTS-ATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTS 120
           L+LT  +TP    + LAK  + SE+ ++  +G       WRK PRLPP+L PDEK  N  
Sbjct: 61  LVLTHPSTPHSFSDGLAKGIKGSEEDKLITDGNSRLGRFWRKPPRLPPRLSPDEKVRNNK 120

Query: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATV 180
              +   L ++  W  RQQKVKDAFIHAWSGYK YAMGYDELMPLS+ G DGLGGLGATV
Sbjct: 121 PIHESEKLNADPKWTERQQKVKDAFIHAWSGYKNYAMGYDELMPLSKRGVDGLGGLGATV 180

Query: 181 VDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGE 240
           VDALDTAMIMGA DIVSEAGSWIEK+L DRI +KGQVNLFETTIR++GGLLSAYHL  GE
Sbjct: 181 VDALDTAMIMGADDIVSEAGSWIEKHLNDRISKKGQVNLFETTIRVLGGLLSAYHLSGGE 240

Query: 241 YGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300
            G    ++GPKP VYLE A+NLADRLL AFTSSPT IP  DVVL D SAH++P G SSTS
Sbjct: 241 QGTDSVHKGPKPIVYLETARNLADRLLLAFTSSPTPIPFSDVVLHDPSAHSSPDGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRL 360
           EVSTLQLEFNYLSTVSGDPKY + AMKVL HMK+LPKVEGLVPIYISP +G+FSGE IRL
Sbjct: 301 EVSTLQLEFNYLSTVSGDPKYGVEAMKVLAHMKNLPKVEGLVPIYISPASGEFSGENIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELP 420
           GSRGDSYYEYL+KVWLQ+ TS ++NFTYL++MYEEA+KGV++LLV+KSIPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLIKVWLQQGTSRDANFTYLYNMYEEAIKGVRNLLVQKSIPNGLVFVGELP 420

Query: 421 YGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCF 480
            G   + SPKMDHLVCFL GTLALGATKGITKE+AM  +LL F+DLENL LAEDLAKTCF
Sbjct: 421 NGPKGSFSPKMDHLVCFLPGTLALGATKGITKEKAMKANLLKFEDLENLKLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLF 540
           EMY VTSTGLAPEIAYFHT+EYSE GL+GGNK SK+IDDIIIK  DRHNL+RPETVESLF
Sbjct: 481 EMYSVTSTGLAPEIAYFHTKEYSEAGLNGGNKSSKFIDDIIIKHADRHNLLRPETVESLF 540

Query: 541 VLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLK 600
           VLYRIT DPKYR WGW+IFEAFE++TKV S GY+SL+DVT +PPRRRDKMETF+LGETLK
Sbjct: 541 VLYRITQDPKYREWGWQIFEAFEKYTKVESAGYSSLEDVTMLPPRRRDKMETFYLGETLK 600

Query: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPMDG 630
           YLYLLFGD++ IPLDK+VFNTEAHP P+ G
Sbjct: 601 YLYLLFGDTNIIPLDKFVFNTEAHPFPIKG 630

BLAST of Cucsa.047700 vs. TAIR10
Match: AT1G30000.1 (AT1G30000.1 alpha-mannosidase 3)

HSP 1 Score: 911.0 bits (2353), Expect = 4.1e-265
Identity = 454/634 (71.61%), Postives = 525/634 (82.81%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYSVKD+HYDNAKFR RS +KV +Q+LLT S KR+Y SCSTGKFL+L+L FG+A 
Sbjct: 1   MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVAC 60

Query: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDE---KGSN 120
           L+L S +P          S  +EK ++   GG+    L RK PRLPP+L PDE   +GS+
Sbjct: 61  LMLMSKSP--------NESGLNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGSS 120

Query: 121 TSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGA 180
           T+     N   S+  W +RQQ VK+AF HAWSGY++YAMGYDELMP+SQ G DGLGGLGA
Sbjct: 121 TNGSTISN---SDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLGA 180

Query: 181 TVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRA 240
           TVVDALDTAMIMG  +IVSEAGSW+E +L++RI +KGQVNLFETTIR++GGLLSAYHL  
Sbjct: 181 TVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSG 240

Query: 241 GEYGKT-FGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSS 300
           GE G     + GPKP +YL IAK+LADRLLSAFTSSPT +P CDV+L +S+AH APGG+S
Sbjct: 241 GEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGAS 300

Query: 301 STSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGET 360
           ST+EV+++QLEFNYLS++SGDPKYS  AMKVL H+K+LPK EGLVPIYISP TG F GE 
Sbjct: 301 STAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGEN 360

Query: 361 IRLGSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVG 420
           IRLGSRGDSYYEYL+KVWLQ+    NSNFTYLHDMY EAMKGV+HLLV+ SIP GLVFVG
Sbjct: 361 IRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFVG 420

Query: 421 ELPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAK 480
           ELPYGS    SPKMDHLVCFL GTLALGATKG+TKE+A+ ++LL+F+DLENL LAEDLAK
Sbjct: 421 ELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLAK 480

Query: 481 TCFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVE 540
           TCFEMYEVT+TGLAPEIAYFHT++Y+E GLDGGNK S Y +DIIIKP DRHNL+RPETVE
Sbjct: 481 TCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETVE 540

Query: 541 SLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGE 600
           SLFVLYRIT D KYR  GW+IFEAFE++TKV SGGYTSLDDVT +PP RRDKMETFFLGE
Sbjct: 541 SLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGE 600

Query: 601 TLKYLYLLFGDSSTIPLDKYVFNTEAHPLPMDGN 631
           TLKYLYLLFGD S IPLDK+VFNTEAHPLP+  N
Sbjct: 601 TLKYLYLLFGDDSVIPLDKFVFNTEAHPLPIRRN 623

BLAST of Cucsa.047700 vs. TAIR10
Match: AT1G51590.1 (AT1G51590.1 alpha-mannosidase 1)

HSP 1 Score: 347.4 bits (890), Expect = 1.8e-95
Identity = 219/494 (44.33%), Postives = 297/494 (60.12%), Query Frame = 1

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIV 195
           R+QKVK+A IHAWS Y++YA G DEL P ++ GTD  GGLGAT+VD+LDT  IMG  +  
Sbjct: 97  RRQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQF 156

Query: 196 SEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEYGKTFGNEGPKPAVYL 255
            +A  W+  +L     +    ++FETTIR+VGGLLSAY L            G K  ++L
Sbjct: 157 QKAREWVASSL--DFDKDYDASMFETTIRVVGGLLSAYDL-----------SGDK--MFL 216

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHA---APGGSSSTSEVSTLQLEFNYLS 315
           E AK++ADRLL A+ ++PT IP   + L++ +AH    A GG S  ++  T QLEF  LS
Sbjct: 217 EKAKDIADRLLPAW-NTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALS 276

Query: 316 TVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLGSRGDSYYEYLLK 375
             +GDPKY     KV+  +      +GL+PIYI+P     S  T   G+ GDS+YEYLLK
Sbjct: 277 QRTGDPKYQQKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLK 336

Query: 376 VWLQ-KRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMD 435
           VW+Q  +TS    +    DM+E++MKG+   LV+KS P+   ++ E    + + +  KMD
Sbjct: 337 VWVQGNKTSAVKPY---RDMWEKSMKGLLS-LVKKSTPSSFTYICE---KNGNNLIDKMD 396

Query: 436 HLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAP 495
            L CF  G LALGA+ G   +E          + + L+LA +LA TC+  Y+ T T LA 
Sbjct: 397 ELACFAPGMLALGAS-GYGPDE----------EKKFLSLAGELAWTCYNFYQSTPTKLAG 456

Query: 496 EIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYR 555
           E  YF T   + Q +  G                  N++RPETVESLF L+R+TG+  Y+
Sbjct: 457 E-NYFFT---AGQDMSVGTS---------------WNILRPETVESLFYLWRLTGNKTYQ 516

Query: 556 AWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKYLYLLFGDSSTI 615
            WGW IF+AFE++++V S GY  L DV T    + +KM++FFL ETLKYLYLLF  SS I
Sbjct: 517 EWGWNIFQAFEKNSRVES-GYVGLKDVNT--GAKDNKMQSFFLAETLKYLYLLFSPSSVI 534

Query: 616 PLDKYVFNTEAHPL 626
            LD++VFNTEAHPL
Sbjct: 577 SLDEWVFNTEAHPL 534

BLAST of Cucsa.047700 vs. TAIR10
Match: AT3G21160.1 (AT3G21160.1 alpha-mannosidase 2)

HSP 1 Score: 340.5 bits (872), Expect = 2.2e-93
Identity = 214/494 (43.32%), Postives = 288/494 (58.30%), Query Frame = 1

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIV 195
           R Q+VK+A +HAWS Y++YA G DEL P ++ G D  GGLGAT++DALDT  IMG  +  
Sbjct: 98  RMQRVKEAMVHAWSSYEKYAWGQDELQPQTKDGVDSFGGLGATMIDALDTLYIMGLDEQF 157

Query: 196 SEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEYGKTFGNEGPKPAVYL 255
            +A  W+  +L D  K+    ++FETTIR+VGGLLSAY L            G K  ++L
Sbjct: 158 QKAREWVASSL-DFDKDYA-ASMFETTIRVVGGLLSAYDL-----------SGDK--IFL 217

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAH--AAPGGSSSTSEVSTLQLEFNYLST 315
           E A ++ADRLL A+  + + IP   + LK  +AH     GG S  ++  T QLEF  LS 
Sbjct: 218 EKAMDIADRLLPAW-DTQSGIPYNIINLKHGNAHNPTWAGGDSILADSGTEQLEFIALSQ 277

Query: 316 VSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLGSRGDSYYEYLLKV 375
            +GDPKY     KV+  +      +GL+PIYI+P T   S  TI  G+ GDS+YEYLLKV
Sbjct: 278 RTGDPKYQQKVEKVISVLNKNFPADGLLPIYINPDTANPSQSTITFGAMGDSFYEYLLKV 337

Query: 376 WLQKRTSGNSNFTYLH--DMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMD 435
           W+     GN      H  DM+E++M G+   LV+KS P    ++ E    S +++  KMD
Sbjct: 338 WV----FGNKTSAVKHYRDMWEKSMNGLLS-LVKKSTPLSFTYICE---KSGNSLIDKMD 397

Query: 436 HLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAP 495
            L CF  G LALGA+     + A  K          L LAE+LA TC+  Y+ T T LA 
Sbjct: 398 ELACFAPGMLALGASG--YSDPAEGKKF--------LTLAEELAWTCYNFYQSTPTKLAG 457

Query: 496 EIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYR 555
           E  +F++      G                      N++RPETVESLF L+R+TG+  Y+
Sbjct: 458 ENYFFNSGSDMSVGTS-------------------WNILRPETVESLFYLWRLTGNKTYQ 517

Query: 556 AWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKYLYLLFGDSSTI 615
            WGW IFEAFE+++++ S GY  L DV T    + +KM++FFL ETLKYLYLLF  ++ I
Sbjct: 518 EWGWNIFEAFEKNSRIES-GYVGLKDVNT--GVKDNKMQSFFLAETLKYLYLLFSPTTVI 535

Query: 616 PLDKYVFNTEAHPL 626
           PLD++VFNTEAHPL
Sbjct: 578 PLDEWVFNTEAHPL 535

BLAST of Cucsa.047700 vs. TAIR10
Match: AT5G43710.1 (AT5G43710.1 Glycosyl hydrolase family 47 protein)

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-31
Identity = 95/267 (35.58%), Postives = 143/267 (53.56%), Query Frame = 1

Query: 137 QQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVS 196
           + +V+  F HA+ GY   A   DEL PLS  G D LGG   T++D+LDT  ++G  +  +
Sbjct: 41  RDEVRGMFYHAFDGYMNNAFPLDELRPLSCQGEDTLGGYALTLIDSLDTLALLGDRERFT 100

Query: 197 EAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEYG-----KTFGNEGPKP 256
            +  WI KNL   I +   V++FETTIR++GGLLSA HL A +Y       ++ NE    
Sbjct: 101 SSVEWIGKNLQFNINK--TVSVFETTIRVLGGLLSA-HLIASDYATGMRIPSYNNE---- 160

Query: 257 AVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYL 316
              L +A+NLA R+L AF  +PT IP   V L            +ST+   TL LEF  L
Sbjct: 161 --LLVLAENLARRMLPAF-DTPTGIPFGSVNLM-YGVDKHESKITSTAGGGTLSLEFGVL 220

Query: 317 STVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLGSRGDSYYEYLL 376
           S ++ DP +   A   +  + +      LV  +I+  TG+++ +   +G+  DS+YEYLL
Sbjct: 221 SRLTNDPVFEQVAKNAVRGLWARRSNLDLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLL 280

Query: 377 KVWLQKRTSGNSNFTYL-HDMYEEAMK 398
           K ++     G+  + Y+  + Y  AM+
Sbjct: 281 KAYI---LFGDEEYLYIFQEAYRSAMQ 293

HSP 2 Score: 67.8 bits (164), Expect = 2.8e-11
Identity = 42/110 (38.18%), Postives = 62/110 (56.36%), Query Frame = 1

Query: 530 MRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKM 589
           +RPE +ES + LY+ T DP+Y   G +   + +   K   G Y  + DV     ++ D M
Sbjct: 373 LRPELIESTYWLYKATRDPRYLDAGRDFVASLQYGAKCPCG-YCHITDVEL--HKQEDHM 432

Query: 590 ETFFLGETLKYLYLLFG---DSSTIPLD---KYVFNTEAHPLPMDGNIQI 634
           E+FFL ET+KYL+LLF    DS  +  +   KY+F+TE H LP+   I +
Sbjct: 433 ESFFLAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEGHLLPITPQISL 479

BLAST of Cucsa.047700 vs. TAIR10
Match: AT1G27520.1 (AT1G27520.1 Glycosyl hydrolase family 47 protein)

HSP 1 Score: 119.4 bits (298), Expect = 8.1e-27
Identity = 90/302 (29.80%), Postives = 148/302 (49.01%), Query Frame = 1

Query: 116 SNTSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGL 175
           S T + +DP++   E      ++KV++ F HA+  Y  YA  +DEL PL++  TD L  L
Sbjct: 17  SLTFFVVDPSSQHIEVKKKQMREKVREMFYHAYDNYMTYAFPHDELKPLTKSFTDSLSEL 76

Query: 176 G---------------ATVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFE 235
           G                T+V++L +  I+G S    +   W+ +NL   I  +  VNLFE
Sbjct: 77  GNLKLEHLPTDYNGSAVTLVESLSSLAILGNSTEFEKGVLWLSENLTFDIDAR--VNLFE 136

Query: 236 TTIRIVGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCD 295
             IR++GGL+SA+ L      +    +G      L +A++L  R L AF  +PT +P   
Sbjct: 137 CNIRVLGGLISAHLLAIDPNNRLI--QGSYNNQLLRLAEDLGKRFLPAF-ETPTGLPYAW 196

Query: 296 VVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGL 355
           + LK+          +STS   +L LE   LS ++GDP++  AA++ L  +  +     L
Sbjct: 197 INLKNGVMENET-TETSTSGCGSLVLEMGALSRLTGDPRFESAALRALRQLWRMRSSLDL 256

Query: 356 VPIYISPHTGKFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNSNF-TYLHDMYEEAMKGV 402
           +   +   TG++   +  +G+  DS+YEYLLK ++     G  ++    H  Y  + K  
Sbjct: 257 LGTTLDVVTGEWIEYSSSIGAGVDSFYEYLLKAYI---LFGKEDYWRMFHSAYLASQKYF 309

HSP 2 Score: 83.2 bits (204), Expect = 6.4e-16
Identity = 48/110 (43.64%), Postives = 65/110 (59.09%), Query Frame = 1

Query: 516 IDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSL 575
           +D  II P  ++  +RPE  ES F LY+ T DP Y   G  + ++   +TKV  GG+ S+
Sbjct: 371 LDHQIIHPTMKYYPLRPELAESTFYLYQATKDPWYLDVGESMVKSLNLYTKVP-GGFASV 430

Query: 576 DDVTTIPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPL 626
            DVTT+  +  D   +FFL ET KYLYLLF D S +    Y+F TE HP+
Sbjct: 431 RDVTTM--QLEDHQHSFFLAETCKYLYLLF-DDSFVAKRNYIFTTEGHPI 476

BLAST of Cucsa.047700 vs. NCBI nr
Match: gi|449433593|ref|XP_004134582.1| (PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis sativus])

HSP 1 Score: 1274.6 bits (3297), Expect = 0.0e+00
Identity = 634/634 (100.00%), Postives = 634/634 (100.00%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY
Sbjct: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
           HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE
Sbjct: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600
           LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 635
           LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 634

BLAST of Cucsa.047700 vs. NCBI nr
Match: gi|659078275|ref|XP_008439639.1| (PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis melo])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 615/634 (97.00%), Postives = 630/634 (99.37%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRSY+KVMTQNLLTGSRKRDYLSCSTGKFL+LL+IFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYLKVMTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAY 60

Query: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           LLLT+ATPVHVPN+ AKVSEN+EK+EIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY
Sbjct: 61  LLLTNATPVHVPNQPAKVSENNEKREIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
           HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSWIEK+LMDRIKEKGQVNLFETTIR+VGGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKHLMDRIKEKGQVNLFETTIRVVGGLLSAYHLRAGEY 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTG+FSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQKRTSGN+NFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           GSDS ISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLL+FDDLENLNLAEDLAKTCFE
Sbjct: 421 GSDSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEIAYFHTEEYSEQG DGGNK SKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGHDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600
           LYRITG+PKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTT+PPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 635
           LYLLFGDSSTIPLDKYVFNTEAHPLPM+GNIQIQ
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQIQ 634

BLAST of Cucsa.047700 vs. NCBI nr
Match: gi|1012248318|ref|XP_015942868.1| (PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Arachis duranensis])

HSP 1 Score: 959.9 bits (2480), Expect = 2.2e-276
Identity = 469/628 (74.68%), Postives = 539/628 (85.83%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS+SLPYS KDV YDNAKFR RS+ KV+TQ+ LT +RKRD  SCSTGKFL+LLLIFG+AY
Sbjct: 1   MSNSLPYSKKDVDYDNAKFRHRSFSKVITQSFLTSNRKRDCFSCSTGKFLLLLLIFGVAY 60

Query: 61  LLLTSATPVHVPNKLAKV-SENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTS 120
           L+LT  +P  V +K   + + NSE   I N  G   + LWR+APRLPP+LPPD KG+N  
Sbjct: 61  LMLTHTSPGDVVSKDQTIGNRNSEGISITNGAG-RLKKLWRRAPRLPPRLPPDAKGTNNG 120

Query: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATV 180
                    + S+WI+R+QKVKDAFIHAWSGYK+YAMG+DELMPLS HGTDGLGGLGATV
Sbjct: 121 IGHATENRDARSMWITRKQKVKDAFIHAWSGYKKYAMGHDELMPLSHHGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGE 240
           VDALDTAMIMG  ++V+EAG+WIE+NL DRI +KGQVNLFETTIR++GGLLSAYHL  GE
Sbjct: 181 VDALDTAMIMGIDEVVAEAGTWIEENLSDRISKKGQVNLFETTIRVLGGLLSAYHLSGGE 240

Query: 241 YGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300
            G    + GPKPAVYLE AKNLADRLLSAFT+SPTAIP  DV+L DSS HAAPGG SST+
Sbjct: 241 KGMNITHAGPKPAVYLETAKNLADRLLSAFTASPTAIPFSDVILHDSSGHAAPGGLSSTA 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRL 360
           EVSTLQLEFNYLS+VSGD KYS+AAMKV++H+K+L K EGLVPIYISPH+G+FSGE  RL
Sbjct: 301 EVSTLQLEFNYLSSVSGDQKYSLAAMKVMDHIKTLSKTEGLVPIYISPHSGQFSGENYRL 360

Query: 361 GSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQ   S +SN +YL+DMY+EAM GV+HLLVRK+IPNGLVF+GELP
Sbjct: 361 GSRGDSYYEYLLKVWLQSGASRDSNTSYLYDMYKEAMNGVRHLLVRKTIPNGLVFIGELP 420

Query: 421 YGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCF 480
           YG +   SPKMDHLVCFLSGTLALGATKG+T+E+AM  ++LNF+DLEN+ LAEDLAKTCF
Sbjct: 421 YGLNGDFSPKMDHLVCFLSGTLALGATKGLTREQAMKNNMLNFEDLENMKLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLF 540
           EMY VTSTGLAPEIAYFHTEEYSEQ LDGGNK S+Y++DIIIKP DRHNL+RPETVESLF
Sbjct: 481 EMYAVTSTGLAPEIAYFHTEEYSEQDLDGGNKSSEYVNDIIIKPADRHNLLRPETVESLF 540

Query: 541 VLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLK 600
           VLYRIT DPKYR WGW+IFEAFE++TKV +GGY SLDDVT+IPP +RDKMETFFLGETLK
Sbjct: 541 VLYRITEDPKYREWGWQIFEAFEKYTKVDTGGYCSLDDVTSIPPHKRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPM 628
           YLYLLF DSS IPLDK+VFNTEAHP+P+
Sbjct: 601 YLYLLFADSSLIPLDKFVFNTEAHPIPI 627

BLAST of Cucsa.047700 vs. NCBI nr
Match: gi|1021581253|ref|XP_016179993.1| (PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Arachis ipaensis])

HSP 1 Score: 957.2 bits (2473), Expect = 1.4e-275
Identity = 464/627 (74.00%), Postives = 535/627 (85.33%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS+SLPYS KDV YDNAKFR RS+ KV+TQ+ LT +RKRD  SCSTGKFL+LLLIFG+AY
Sbjct: 1   MSNSLPYSKKDVDYDNAKFRHRSFSKVITQSFLTSNRKRDCFSCSTGKFLLLLLIFGVAY 60

Query: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           L+LT  +P  V +K   +   + +     NG    + LWR+APRLPP+LPPD KG+N   
Sbjct: 61  LMLTHTSPGDVVSKDQTIGNRNREGISITNGAGRLKKLWRRAPRLPPRLPPDAKGTNNGI 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
                   + ++WI+R+QKVKDAFIHAWSGYK+YAMG+DELMPLS HGTDGLGGLGATVV
Sbjct: 121 GHATENRDARTMWITRKQKVKDAFIHAWSGYKKYAMGHDELMPLSHHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240
           DALDTAMIMG  ++V+EAG+WIE+NL DRI +KGQVNLFETTIR++GGLLSAYHL  GE 
Sbjct: 181 DALDTAMIMGIDEVVAEAGTWIEENLSDRISKKGQVNLFETTIRVLGGLLSAYHLSGGEK 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           G    + GPKPAVYLE AKNLADRLLSAFT+SPTAIP  DV+L DSS  AAPGG SST+E
Sbjct: 241 GMNITHAGPKPAVYLETAKNLADRLLSAFTASPTAIPFSDVILHDSSGRAAPGGLSSTAE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360
           VSTLQLEFNYLS+VSGD KYS+ AMKV++H+K+LPK EGLVPIYISPH+G+FSGE  RLG
Sbjct: 301 VSTLQLEFNYLSSVSGDQKYSLEAMKVMDHIKTLPKTEGLVPIYISPHSGQFSGENYRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQ   S +SN +YL+DMY+EAM GV+HLLVRK+IPNGLVF+GELPY
Sbjct: 361 SRGDSYYEYLLKVWLQSGASRDSNTSYLYDMYKEAMNGVRHLLVRKTIPNGLVFIGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           G +   SPKMDHLVCFLSGTLALGATKG+TKE+AM  ++LNF+DLEN+ LAEDLAKTCFE
Sbjct: 421 GLNGDFSPKMDHLVCFLSGTLALGATKGLTKEQAMKNNMLNFEDLENMKLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MY VTSTGLAPEIAYFHTEEYSEQ LDGGNK S+Y++DIIIKP DRHNL+RPETVESLFV
Sbjct: 481 MYAVTSTGLAPEIAYFHTEEYSEQDLDGGNKSSEYVNDIIIKPADRHNLLRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600
           LYRIT DPKYR WGW+IFEAFE++TKV +GGY SLDDVT+IPP +RDKMETFFLGETLKY
Sbjct: 541 LYRITEDPKYREWGWQIFEAFEKYTKVDTGGYCSLDDVTSIPPHKRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPM 628
           LYLLF DSS IPLDK+VFNTEAHP+P+
Sbjct: 601 LYLLFSDSSLIPLDKFVFNTEAHPIPI 627

BLAST of Cucsa.047700 vs. NCBI nr
Match: gi|566166397|ref|XP_002306001.2| (glycoside hydrolase family 47 family protein [Populus trichocarpa])

HSP 1 Score: 954.9 bits (2467), Expect = 7.1e-275
Identity = 472/632 (74.68%), Postives = 537/632 (84.97%), Query Frame = 1

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYS+KDVHYDNAKFRQRS+VKV+TQ+LLT + K D LSCSTGKFL LL+IFGLA 
Sbjct: 1   MSKSLPYSMKDVHYDNAKFRQRSFVKVITQSLLTSNAKHDCLSCSTGKFLALLMIFGLAC 60

Query: 61  LLLT-SATPVHVPNKLAK-VSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNT 120
           L+ T  +TP  V    A+ +  N E + I ++GG  F+  WRK PRLPPQLPPDEKGS  
Sbjct: 61  LMFTHKSTPHSVSQGQAEGIKVNKEVKGISDSGG-RFQRFWRKPPRLPPQLPPDEKGSIG 120

Query: 121 SYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGAT 180
           +   +   L ++S W+ RQQ+V+ AFIHAWSGYK YAMGYDELMPLSQ G DGLGGLGAT
Sbjct: 121 NLPHELQKLNTDSKWVDRQQRVQKAFIHAWSGYKMYAMGYDELMPLSQRGVDGLGGLGAT 180

Query: 181 VVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAG 240
           VVDALDTA+IMG  ++VSEAG WIE +L DRI +KGQVNLFETTIR++GGLLSAYH   G
Sbjct: 181 VVDALDTALIMGVDEVVSEAGLWIETHLSDRISKKGQVNLFETTIRVLGGLLSAYHFSGG 240

Query: 241 EYGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSST 300
           E G    ++GPKP VYLE A+NLADRLL AFTSSPT+IP  DVVL D SAH AP G SST
Sbjct: 241 EQGTNLTHKGPKPTVYLETARNLADRLLLAFTSSPTSIPFSDVVLHDPSAHRAPDGLSST 300

Query: 301 SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIR 360
           +EVST+QLEFNYLSTVSGDP+YSI AMKVLEH+K LPKVEGLVPIYISP +G+FSGE IR
Sbjct: 301 AEVSTVQLEFNYLSTVSGDPRYSIEAMKVLEHIKDLPKVEGLVPIYISPDSGEFSGENIR 360

Query: 361 LGSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGEL 420
           LGSRGDSYYEYL+KVWLQ+  + +SNFTYL+DMYEEAMKGV+HLLV+KSIPNGLVFVGEL
Sbjct: 361 LGSRGDSYYEYLIKVWLQQGRNRDSNFTYLYDMYEEAMKGVRHLLVQKSIPNGLVFVGEL 420

Query: 421 PYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTC 480
           PYG   + SPKMDHLVCFL GTLALGATKG+TKE+AM ++LL F+DLENL LAEDLAKTC
Sbjct: 421 PYGPKGSFSPKMDHLVCFLPGTLALGATKGLTKEKAMKENLLKFEDLENLKLAEDLAKTC 480

Query: 481 FEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESL 540
           FEMY VTSTGLAPEIAYFHTEEYSE GLDGGNK SK+++DIIIK  DRHNL+RPETVESL
Sbjct: 481 FEMYSVTSTGLAPEIAYFHTEEYSEHGLDGGNKNSKFVNDIIIKHADRHNLLRPETVESL 540

Query: 541 FVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETL 600
           F+LYRIT DPKY+ WGW+IFEAFE++TKV SGGY+SL+DVT +PPRRRDKMETFFLGETL
Sbjct: 541 FILYRITEDPKYQEWGWQIFEAFEKYTKVDSGGYSSLEDVTVLPPRRRDKMETFFLGETL 600

Query: 601 KYLYLLFGDSSTIPLDKYVFNTEAHPLPMDGN 631
           KY YLLFGD S IPLDKYVFNTEAHPLP+ G+
Sbjct: 601 KYFYLLFGDRSVIPLDKYVFNTEAHPLPIKGS 631