Cucsa.046250 (gene) Cucumber (Gy14) v1

NameCucsa.046250
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProtein kinase
Locationscaffold00542 : 1812918 .. 1816506 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTGTGGATCTCTGTGTGGGATTTGCCAGGCTTTTTACCCAAGGTGGAGTTTGACTTTGAAACCAAATTAAAAAGATGGATTAAAGGAGGAAGGGGAAGTTTGGTGTTAAACAGAACTCTGCTGCTTTGTGGCTTGAGAGTCATCCCTGTTTCTGGTTGTTGGTTTTTGCTTTAAGCGATTGTTTTGCTGGTGGGAATGAATAGGATTTTGGGGGGATCCAGAATCTCGATTATTTATTGAGTTGGGTCTTTGTGGATCTCAATGCTTCTTTAATTCGATGAAACAAAGAATGTTTCTTCCTTAGTCTTGATGGGTTTGAAGAAATCCAGCTTTTGGGGCCATCAAATTAATCATACCCTTTTGTTTCCGAGAACAACTTCAGTTTAGTCCTCTTCCTGAGCAAGTGGGGGAGAAAATCAAGGACGTTTGTTGTTGCAAGATCTTTTGAGAGGTCCATCTAGGAATTATAGGAATCGGGTGGAAAAAAATGGACGAAGAGGCTAATTCTTGGCTTAGGAGAACGAAGTTTTCTCACACGGTTTATCATCGATGGGACTCATTAAAATTAAACCCAGTTCCTTTCATCGTCGAACCACCTCGCAATTCTGGCTTGAAATCGAGGCCTCCATCGGCTTCATCTGCTCAGAAGCCGAATCCTGATATTTCGAAGATTCAGCGAAGTTTCATTTCCAATAAACAGAGGTCTCTGTCTCCTCTCCCTGAGAGCAACCTCTCTGAAGTTTTCAAGGAAGCAAAATCCGAGAGTAAGAGATTCTCAACTCCAACTCCTCGACTTAGAGAACGAACTAAGGAATTCAAGAACAAGCTATTCAATAAGGACCCTCAAGATTCAAAATCATCCAATTCCAAGTCATCGTTGAATACCAGTCCCTTGAAACAACTATCTTCAGGGAAAGGGGGTGATAAGTCAAAGTTGAAGAAGGACTCTTCCTGGACTAAATATTTCGATTCTGGAAAAGTCACTGCGGTTGAAACAGCGGATGACTGGACTGTTGATCTCTCCAAGTTGTTTGTTGGATTAAGGTTTGCTCATGGAGCTCATAGTCGACTTTATCATGGAAAGTACAACGACGAACCTGTTGCTGTTAAAATTATTAGAGTGCCTGATGATGACGAAAACGGAACGCTTGCGGCTCGATTGGAGAAGCAATTCACCAGAGAAGTTACCCTTTTATCGCGTCTCTATCATCCAAATGTTATAAAGGTAATTCAGTTGTTAGATTGTAAGTGATGTTTTGAACTACATAGCAACTTATTAGTTTTCTTGATCTTTGTTTGTTTTCTGTTGCAGTTTGTAGCTGCTTGTAGGAACCCACCTGTATACTGTGTCATTACTGAATATCTGTCTCAAGGATCATTAAGGGCATACTTACACAAACTTGAGCACCAATCCCTCCCTTTGCAAAAACTAATTAAATTTGCTTTAGATGTCGCTCGTGGCATGGAATACCTTCACTCACAAGGTGTAATTCATCGGGATCTTAAGCCTGAGAATGTTCTTATTGATGAAGACATGCACTTGAAAATTGCAGATTTTGGTATAGCTTGTCCTGAGGCCTTTTTTGACCCATTAGCTGATGATCCCGGAACTTACCGATGGATGGCACCGGAGATGATCAAACACAAACCTTGTAGTCGAAAGGTTGACGTGTACAGCTTTGGGCTTATGTTATGGGAAATGGTATCTGGTGCAATTCCATACGAGGATATGACTCCCATTCAAGCAGCCTTTGCTGTTGTGAATAAGGTTAGTTTTAATTTGATCACGTGTTCCTTGACCATCTTCTTCTTTAGGTTTATCATGTGACTCATGGTGTTTCATTGATACCCATTCTATGTTTTCTTCGGAAAGTTCACATGTTCCATGTTTATATTGTGAGTGCCTCAATATCCTGCATGTGACCAGATGAAACTTGTGCTTATTCATCGAATTTACTCGTCTTTTTGAATCTCATTCCTCGTAGTCCCAAAAATATCAACGCTTGTTATGGGATGCTGTTTAACTGTAAAGTTGAAGATGAAATTTAATTAGTTATCAGATAATATCTTTGAAAGAAAAGTCTTTGAATCTGAGAAGCAAGTCATCACAATTGGCAGAAAACTGAGTTCGTACACATCCATTATTGATGATAAGGTATCCCTTCGTTATAGGTAGTAGATGAAGTTTCATTTGAGGTTTGGCAGTATTCAGTGTACAGTAGGTTTTGTTGAAGAATATGCTCAAGCAACTAGTTTGAAAAAAAAAACCAAGTTTTCGATGGAGATTGTTTAGTCCCAAAGCACGGGGCCACTTAGTCATGGTTTAAAATACTATACGGTTTTCTCGGCACCAAATGTTGTTGTAAATGAGCTTTTTTTTTTTTTTGCAATTTATCTTTAGTGAAAGTAAGTGTACAGGTTATTTTTGTTAGATTTTCTTAGATACCCCAAGTTTCTCAATAATAGAAGTGGTTTACAATCTTTTTATATTGCATTCTTTCAACAAAACTAAAAAAACTGTGCTCATTTTGTTTTTCAGAATCTTAGGCCTGTTATCTCAAGTGATTGTCCATTAGCAATGCGAGCTTTAATCGAGCAATGTTGGTCGCTACAACCGGACAAGAGACCTGATTTCTGGCAGATTGTGAAGGTATTAGAGCAATTCGAATCTTCGCTTGCTCGTGATGGGACACTGAATTTATTAGGTAACCCTCTGTCTAGTTTCCATGACCATAAGAAAGGGCTCCTCCATTGGATTCAAAAGCTTGGCCCCCTACATCCCGAAGTTTCATCCTCGCCCGTGCCAAAACCAAAATTCTCATAAGTCGTTCTCCATGTTACAACCTAGCTTTCGTAGTGTTCTTGTAGGCAAGTTTTTGCATTTTGTAATTAAAACTAGACGTTGTTCACTTCCCTTTATTTGGCGAGTTTATGATGAATTTGTGTATTTAGCATACTATTCCTGCCTTGATTCGATTCTTGTCTTGCAATAATCAAATGGTCAGCATCTTGTAGGATCAAACCATATTGTGATAAACACAAGTAAGAAAAAGCAGAAGTGCCGTCTTTCTTCGTCT

mRNA sequence

TATTGTGGATCTCTGTGTGGGATTTGCCAGGCTTTTTACCCAAGGTGGAGTTTGACTTTGAAACCAAATTAAAAAGATGGATTAAAGGAGGAAGGGGAAGTTTGGTGTTAAACAGAACTCTGCTGCTTTGTGGCTTGAGAGTCATCCCTGTTTCTGGTTGTTGGTTTTTGCTTTAAGCGATTGTTTTGCTGGTGGGAATGAATAGGATTTTGGGGGGATCCAGAATCTCGATTATTTATTGAGTTGGGTCTTTGTGGATCTCAATGCTTCTTTAATTCGATGAAACAAAGAATGTTTCTTCCTTAGTCTTGATGGGTTTGAAGAAATCCAGCTTTTGGGGCCATCAAATTAATCATACCCTTTTGTTTCCGAGAACAACTTCAGTTTAGTCCTCTTCCTGAGCAAGTGGGGGAGAAAATCAAGGACGTTTGTTGTTGCAAGATCTTTTGAGAGGTCCATCTAGGAATTATAGGAATCGGGTGGAAAAAAATGGACGAAGAGGCTAATTCTTGGCTTAGGAGAACGAAGTTTTCTCACACGGTTTATCATCGATGGGACTCATTAAAATTAAACCCAGTTCCTTTCATCGTCGAACCACCTCGCAATTCTGGCTTGAAATCGAGGCCTCCATCGGCTTCATCTGCTCAGAAGCCGAATCCTGATATTTCGAAGATTCAGCGAAGTTTCATTTCCAATAAACAGAGGTCTCTGTCTCCTCTCCCTGAGAGCAACCTCTCTGAAGTTTTCAAGGAAGCAAAATCCGAGAGTAAGAGATTCTCAACTCCAACTCCTCGACTTAGAGAACGAACTAAGGAATTCAAGAACAAGCTATTCAATAAGGACCCTCAAGATTCAAAATCATCCAATTCCAAGTCATCGTTGAATACCAGTCCCTTGAAACAACTATCTTCAGGGAAAGGGGGTGATAAGTCAAAGTTGAAGAAGGACTCTTCCTGGACTAAATATTTCGATTCTGGAAAAGTCACTGCGGTTGAAACAGCGGATGACTGGACTGTTGATCTCTCCAAGTTGTTTGTTGGATTAAGGTTTGCTCATGGAGCTCATAGTCGACTTTATCATGGAAAGTACAACGACGAACCTGTTGCTGTTAAAATTATTAGAGTGCCTGATGATGACGAAAACGGAACGCTTGCGGCTCGATTGGAGAAGCAATTCACCAGAGAAGTTACCCTTTTATCGCGTCTCTATCATCCAAATGTTATAAAGTTTGTAGCTGCTTGTAGGAACCCACCTGTATACTGTGTCATTACTGAATATCTGTCTCAAGGATCATTAAGGGCATACTTACACAAACTTGAGCACCAATCCCTCCCTTTGCAAAAACTAATTAAATTTGCTTTAGATGTCGCTCGTGGCATGGAATACCTTCACTCACAAGGTGTAATTCATCGGGATCTTAAGCCTGAGAATGTTCTTATTGATGAAGACATGCACTTGAAAATTGCAGATTTTGGTATAGCTTGTCCTGAGGCCTTTTTTGACCCATTAGCTGATGATCCCGGAACTTACCGATGGATGGCACCGGAGATGATCAAACACAAACCTTGTAGTCGAAAGGTTGACGTGTACAGCTTTGGGCTTATGTTATGGGAAATGGTATCTGGTGCAATTCCATACGAGGATATGACTCCCATTCAAGCAGCCTTTGCTGTTGTGAATAAGAATCTTAGGCCTGTTATCTCAAGTGATTGTCCATTAGCAATGCGAGCTTTAATCGAGCAATGTTGGTCGCTACAACCGGACAAGAGACCTGATTTCTGGCAGATTGTGAAGGTATTAGAGCAATTCGAATCTTCGCTTGCTCGTGATGGGACACTGAATTTATTAGGTAACCCTCTGTCTAGTTTCCATGACCATAAGAAAGGGCTCCTCCATTGGATTCAAAAGCTTGGCCCCCTACATCCCGAAGTTTCATCCTCGCCCGTGCCAAAACCAAAATTCTCATAAGTCGTTCTCCATGTTACAACCTAGCTTTCGTAGTGTTCTTGTAGGCAAGTTTTTGCATTTTGTAATTAAAACTAGACGTTGTTCACTTCCCTTTATTTGGCGAGTTTATGATGAATTTGTGTATTTAGCATACTATTCCTGCCTTGATTCGATTCTTGTCTTGCAATAATCAAATGGTCAGCATCTTGTAGGATCAAACCATATTGTGATAAACACAAGTAAGAAAAAGCAGAAGTGCCGTCTttcttcgtct

Coding sequence (CDS)

ATGGACGAAGAGGCTAATTCTTGGCTTAGGAGAACGAAGTTTTCTCACACGGTTTATCATCGATGGGACTCATTAAAATTAAACCCAGTTCCTTTCATCGTCGAACCACCTCGCAATTCTGGCTTGAAATCGAGGCCTCCATCGGCTTCATCTGCTCAGAAGCCGAATCCTGATATTTCGAAGATTCAGCGAAGTTTCATTTCCAATAAACAGAGGTCTCTGTCTCCTCTCCCTGAGAGCAACCTCTCTGAAGTTTTCAAGGAAGCAAAATCCGAGAGTAAGAGATTCTCAACTCCAACTCCTCGACTTAGAGAACGAACTAAGGAATTCAAGAACAAGCTATTCAATAAGGACCCTCAAGATTCAAAATCATCCAATTCCAAGTCATCGTTGAATACCAGTCCCTTGAAACAACTATCTTCAGGGAAAGGGGGTGATAAGTCAAAGTTGAAGAAGGACTCTTCCTGGACTAAATATTTCGATTCTGGAAAAGTCACTGCGGTTGAAACAGCGGATGACTGGACTGTTGATCTCTCCAAGTTGTTTGTTGGATTAAGGTTTGCTCATGGAGCTCATAGTCGACTTTATCATGGAAAGTACAACGACGAACCTGTTGCTGTTAAAATTATTAGAGTGCCTGATGATGACGAAAACGGAACGCTTGCGGCTCGATTGGAGAAGCAATTCACCAGAGAAGTTACCCTTTTATCGCGTCTCTATCATCCAAATGTTATAAAGTTTGTAGCTGCTTGTAGGAACCCACCTGTATACTGTGTCATTACTGAATATCTGTCTCAAGGATCATTAAGGGCATACTTACACAAACTTGAGCACCAATCCCTCCCTTTGCAAAAACTAATTAAATTTGCTTTAGATGTCGCTCGTGGCATGGAATACCTTCACTCACAAGGTGTAATTCATCGGGATCTTAAGCCTGAGAATGTTCTTATTGATGAAGACATGCACTTGAAAATTGCAGATTTTGGTATAGCTTGTCCTGAGGCCTTTTTTGACCCATTAGCTGATGATCCCGGAACTTACCGATGGATGGCACCGGAGATGATCAAACACAAACCTTGTAGTCGAAAGGTTGACGTGTACAGCTTTGGGCTTATGTTATGGGAAATGGTATCTGGTGCAATTCCATACGAGGATATGACTCCCATTCAAGCAGCCTTTGCTGTTGTGAATAAGAATCTTAGGCCTGTTATCTCAAGTGATTGTCCATTAGCAATGCGAGCTTTAATCGAGCAATGTTGGTCGCTACAACCGGACAAGAGACCTGATTTCTGGCAGATTGTGAAGGTATTAGAGCAATTCGAATCTTCGCTTGCTCGTGATGGGACACTGAATTTATTAGGTAACCCTCTGTCTAGTTTCCATGACCATAAGAAAGGGCTCCTCCATTGGATTCAAAAGCTTGGCCCCCTACATCCCGAAGTTTCATCCTCGCCCGTGCCAAAACCAAAATTCTCATAA

Protein sequence

MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDISKIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQDSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPEVSSSPVPKPKFS*
BLAST of Cucsa.046250 vs. Swiss-Prot
Match: HT1_ARATH (Serine/threonine-protein kinase HT1 OS=Arabidopsis thaliana GN=HT1 PE=1 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.4e-92
Identity = 175/381 (45.93%), Postives = 245/381 (64.30%), Query Frame = 1

Query: 114 LFNKDPQDSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETA-- 173
           L +K P +    N   + ++S   + +  +  +K +     SW+   +S  V   E +  
Sbjct: 15  LRSKLPLEPSLPNLPCNPSSSKTNRYAEAETMEKKRFDSMESWSMILESENVETWEASKG 74

Query: 174 --DDWTVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQF 233
             ++WT DLS+LF+G +FA GAHSR+Y G Y    VAVK++R+P   E     A+LE+QF
Sbjct: 75  EREEWTADLSQLFIGNKFASGAHSRIYRGIYKQRAVAVKMVRIPTHKEE--TRAKLEQQF 134

Query: 234 TREVTLLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKF 293
             EV LLSRL+HPN+++F+AAC+ PPVYC+ITEY+SQG+LR YL+K E  SL ++ +++ 
Sbjct: 135 KSEVALLSRLFHPNIVQFIAACKKPPVYCIITEYMSQGNLRMYLNKKEPYSLSIETVLRL 194

Query: 294 ALDVARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRW 353
           ALD++RGMEYLHSQGVIHRDLK  N+L++++M +K+ADFG +C E        + GTYRW
Sbjct: 195 ALDISRGMEYLHSQGVIHRDLKSNNLLLNDEMRVKVADFGTSCLETQCREAKGNMGTYRW 254

Query: 354 MAPEMIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCP 413
           MAPEMIK KP +RKVDVYSFG++LWE+ +  +P++ MTP+QAAFAV  KN RP + + C 
Sbjct: 255 MAPEMIKEKPYTRKVDVYSFGIVLWELTTALLPFQGMTPVQAAFAVAEKNERPPLPASCQ 314

Query: 414 LAMRALIEQCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLN---LLGNPLSSFHDHKKG 473
            A+  LI++CWS  P KRPDF  IV VLE+++  +     L     L     +  DH KG
Sbjct: 315 PALAHLIKRCWSENPSKRPDFSNIVAVLEKYDECVKEGLPLTSHASLTKTKKAILDHLKG 374

Query: 474 LLHWIQKLGPLHPEVSSSPVP 488
            +  I          SSS VP
Sbjct: 375 CVTSISS------PFSSSSVP 387

BLAST of Cucsa.046250 vs. Swiss-Prot
Match: STY46_ARATH (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana GN=STY46 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.6e-61
Identity = 113/277 (40.79%), Postives = 175/277 (63.18%), Query Frame = 1

Query: 172 DDWTVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTR 231
           D W ++L  L  G + A G++  LY G Y  + VA+K+++    D +      LEK+F +
Sbjct: 281 DVWEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLKPERLDSD------LEKEFAQ 340

Query: 232 EVTLLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFAL 291
           EV ++ ++ H NV++F+ AC  PP  C++TE++  GS+  YLHK +     L  L K A+
Sbjct: 341 EVFIMRKVRHKNVVQFIGACTKPPHLCIVTEFMPGGSVYDYLHK-QKGVFKLPTLFKVAI 400

Query: 292 DVARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMA 351
           D+ +GM YLH   +IHRDLK  N+L+DE+  +K+ADFG+A  +A    +  + GTYRWMA
Sbjct: 401 DICKGMSYLHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTGVMTAETGTYRWMA 460

Query: 352 PEMIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 411
           PE+I+HKP   K DV+S+G++LWE+++G +PYE MTP+QAA  VV K LRP I  +    
Sbjct: 461 PEVIEHKPYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPK 520

Query: 412 MRALIEQCWSLQPDKRPDFWQIVKVLEQFESSLARDG 449
           +  L+E+ W     +RPDF +I++ L++    +  +G
Sbjct: 521 LAELLERLWEHDSTQRPDFSEIIEQLQEIAKEVGEEG 550

BLAST of Cucsa.046250 vs. Swiss-Prot
Match: STY8_ARATH (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana GN=STY8 PE=1 SV=2)

HSP 1 Score: 236.9 bits (603), Expect = 4.8e-61
Identity = 117/304 (38.49%), Postives = 190/304 (62.50%), Query Frame = 1

Query: 136 LKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSKLFVGLRFAHGAHSRL 195
           LK     K    S  + D S  +   +      +  D+W +D+++L +  + A G++  L
Sbjct: 241 LKDQPGSKQKSISFFEHDKSSNELIPACIEIPTDGTDEWEIDVTQLKIEKKVASGSYGDL 300

Query: 196 YHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLYHPNVIKFVAACRNPP 255
           + G Y  + VA+K ++ PD   N  L     ++F++EV ++ ++ H NV++F+ AC   P
Sbjct: 301 HRGTYCSQEVAIKFLK-PDRVNNEML-----REFSQEVFIMRKVRHKNVVQFLGACTRSP 360

Query: 256 VYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYLHSQGVIHRDLKPENV 315
             C++TE++++GS+  +LHK +  +  LQ L+K ALDVA+GM YLH   +IHRDLK  N+
Sbjct: 361 TLCIVTEFMARGSIYDFLHK-QKCAFKLQTLLKVALDVAKGMSYLHQNNIIHRDLKTANL 420

Query: 316 LIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPCSRKVDVYSFGLMLWE 375
           L+DE   +K+ADFG+A  +     +  + GTYRWMAPE+I+HKP + K DV+S+ ++LWE
Sbjct: 421 LMDEHGLVKVADFGVARVQIESGVMTAETGTYRWMAPEVIEHKPYNHKADVFSYAIVLWE 480

Query: 376 MVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCWSLQPDKRPDFWQIVK 435
           +++G IPY  +TP+QAA  VV K LRP I       ++ L+E+CW   P++RP F +I++
Sbjct: 481 LLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPKVKGLLERCWHQDPEQRPLFEEIIE 537

Query: 436 VLEQ 440
           +L+Q
Sbjct: 541 MLQQ 537

BLAST of Cucsa.046250 vs. Swiss-Prot
Match: STY17_ARATH (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana GN=STY17 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 9.0e-60
Identity = 118/334 (35.33%), Postives = 197/334 (58.98%), Query Frame = 1

Query: 121 DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKK------DSSWTKYFDSGKVTAVETADDW 180
           D  S      L  +  K++   K    SK K       D S  +   +      +  D+W
Sbjct: 226 DGWSQEETEGLKDALKKEIRKFKDQPCSKQKSITFFEHDKSTNELLPACVEIPTDGTDEW 285

Query: 181 TVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVT 240
            +D+ +L +  + A G++  L+ G Y  + VA+KI++ P+      + A + ++F++EV 
Sbjct: 286 EIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILK-PE-----RVNAEMLREFSQEVY 345

Query: 241 LLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSL-PLQKLIKFALDV 300
           ++ ++ H NV++F+ AC   P  C++TE++++GS+  +LHK  H+ +  +Q L+K ALDV
Sbjct: 346 IMRKVRHKNVVQFIGACTRSPNLCIVTEFMTRGSIYDFLHK--HKGVFKIQSLLKVALDV 405

Query: 301 ARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPE 360
           ++GM YLH   +IHRDLK  N+L+DE   +K+ADFG+A  +     +  + GTYRWMAPE
Sbjct: 406 SKGMNYLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTESGVMTAETGTYRWMAPE 465

Query: 361 MIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMR 420
           +I+HKP   + DV+S+ ++LWE+++G +PY  +TP+QAA  VV K LRP I  +    + 
Sbjct: 466 VIEHKPYDHRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLT 525

Query: 421 ALIEQCWSLQPDKRPDFWQIVKVLEQFESSLARD 448
            L+E+CW   P  RP+F +I+++L Q    +  D
Sbjct: 526 ELLEKCWQQDPALRPNFAEIIEMLNQLIREVGDD 551

BLAST of Cucsa.046250 vs. Swiss-Prot
Match: Y9955_DICDI (Probable serine/threonine-protein kinase DDB_G0267514 OS=Dictyostelium discoideum GN=DDB_G0267514 PE=3 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.4e-51
Identity = 116/331 (35.05%), Postives = 184/331 (55.59%), Query Frame = 1

Query: 111 KNKLFNKDPQDSKSSNSKSSLNTSPLKQLSSGK--GGDKSKLKKDSSWTKYFDSGKVTAV 170
           +N +FN +    ++ N+    N SP +  + G       S ++  S   +YF        
Sbjct: 605 QNNIFNHN----QNQNNHLINNHSPNQYNNQGNILKNSGSVVEPPSQQQQYFS------- 664

Query: 171 ETADDWTVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQ 230
               D  +  S+L +  +   G    +Y G +    VA+K I++ +D  N  L     ++
Sbjct: 665 ----DIEISFSELKISSKLGEGTFGVVYKGLWRGSSVAIKQIKINEDVNNQVL-----EE 724

Query: 231 FTREVTLLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIK 290
           F +E+T+LSRL HPN++  +AAC  PP  C ITEYL  GSL   LH  + + + +Q   K
Sbjct: 725 FRKELTILSRLRHPNIVLLMAACTAPPNLCFITEYLPGGSLYDALHSKKIK-MNMQLYKK 784

Query: 291 FALDVARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYR 350
            A+ +A+GM YLH  GVIHRD+K  N+L+DE M++KI DFG++  ++    +    G+  
Sbjct: 785 LAIQIAQGMNYLHLSGVIHRDIKSLNLLLDEHMNVKICDFGLSKLKSKSTEMTKSIGSPI 844

Query: 351 WMAPEMIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDC 410
           WM+PE++  +  + KVDVY+FG++LWE+ +G +PY  +  +Q A AV  K+LRP I +  
Sbjct: 845 WMSPELLMGEDYTEKVDVYAFGIILWELGTGELPYSGLDSVQLALAVTTKSLRPPIPNAW 904

Query: 411 PLAMRALIEQCWSLQPDKRPDFWQIVKVLEQ 440
           P  +  LI+ CW   P KRP F +I+ +L +
Sbjct: 905 PYQLSHLIQACWHQDPLKRPSFTEILNLLNE 914

BLAST of Cucsa.046250 vs. TrEMBL
Match: A0A0A0KNC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G517390 PE=4 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 3.2e-290
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS 60
           MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS 60

Query: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ 120
           KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ
Sbjct: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ 120

Query: 121 DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSK 180
           DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSK
Sbjct: 121 DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSK 180

Query: 181 LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLY 240
           LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLY
Sbjct: 181 LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLY 240

Query: 241 HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL 300
           HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL
Sbjct: 241 HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL 300

Query: 301 HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC 360
           HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC
Sbjct: 301 HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC 360

Query: 361 SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW 420
           SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW
Sbjct: 361 SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW 420

Query: 421 SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPE 480
           SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPE
Sbjct: 421 SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPE 480

Query: 481 VSSSPVPKPKFS 493
           VSSSPVPKPKFS
Sbjct: 481 VSSSPVPKPKFS 492

BLAST of Cucsa.046250 vs. TrEMBL
Match: Q9FDV7_FAGSY (Protein kinase (PK) (Fragment) OS=Fagus sylvatica GN=pk1 PE=2 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 4.3e-202
Identity = 358/494 (72.47%), Postives = 405/494 (81.98%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS 60
           M EE NSW+RRTKFSHTV HR D+ +L   P I+ P RNSGLKSRP + SS++K      
Sbjct: 1   MGEEGNSWIRRTKFSHTVCHRLDASRLASFPIIIRPERNSGLKSRPGATSSSEKFGT--- 60

Query: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ 120
                 I NKQRSLSPLPES LS+VFKEA+SE+KRF TP PR RE+ K    K  +KD  
Sbjct: 61  ------IYNKQRSLSPLPESRLSDVFKEARSETKRFMTPLPRRREQAKGVMGKFVHKDFH 120

Query: 121 DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFD--SGKVTAVETADDWTVDL 180
           + K+S+SKS  N+S L+ LSS K  DKSK +K+SSW KYFD   G+V AVET+D+WTVDL
Sbjct: 121 EVKASDSKSLANSSALRHLSSMKVNDKSKYRKESSWAKYFDHGGGRVNAVETSDEWTVDL 180

Query: 181 SKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSR 240
           SKLF+GL+FAHGAHSRLYHG YNDEPVAVKIIRVP+DDENG L ARLEKQF REVTLLSR
Sbjct: 181 SKLFLGLKFAHGAHSRLYHGIYNDEPVAVKIIRVPEDDENGALGARLEKQFNREVTLLSR 240

Query: 241 LYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGME 300
           L+  N+IKFVAACR PPVYCV+TEYLS+GSLRAYLHKLE +SLPLQKLI FALD+ARGME
Sbjct: 241 LHFHNIIKFVAACRKPPVYCVVTEYLSEGSLRAYLHKLERKSLPLQKLIAFALDIARGME 300

Query: 301 YLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHK 360
           Y+HSQGVIHRDLKPENVLID++ HLKIADFGIAC EA+ D LADDPGTYRWMAPEMIKHK
Sbjct: 301 YIHSQGVIHRDLKPENVLIDQEFHLKIADFGIACEEAYCDSLADDPGTYRWMAPEMIKHK 360

Query: 361 PCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQ 420
              RKVDVYSFGL+LWEMV+G IPYEDM P+QAAFAVVNKNLRPVI   CP AMRALIEQ
Sbjct: 361 SYGRKVDVYSFGLILWEMVAGTIPYEDMNPVQAAFAVVNKNLRPVIPRYCPPAMRALIEQ 420

Query: 421 CWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLH 480
           CWSLQ +KRP+FWQ+VKVLEQFESSLARDGTLNL+ +   +  DHKKGLLHWI KLGP+H
Sbjct: 421 CWSLQSEKRPEFWQVVKVLEQFESSLARDGTLNLVQS--LTCQDHKKGLLHWIHKLGPVH 480

Query: 481 PEVSSSPVPKPKFS 493
           P  S   +PKPK +
Sbjct: 481 PNGS---MPKPKLT 480

BLAST of Cucsa.046250 vs. TrEMBL
Match: A0A061GMB7_THECC (Tyrosine kinase, putative OS=Theobroma cacao GN=TCM_029853 PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 2.1e-201
Identity = 356/500 (71.20%), Postives = 413/500 (82.60%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNS----GLKSRPPSASSAQKPN 60
           M ++ANSW+RRTKFSHTV HR DS +L   PF ++  RN     GLK R   AS+ QK  
Sbjct: 1   MADDANSWIRRTKFSHTVCHRLDSSRLVSFPFHLQSDRNLEWSLGLKPRSAVASAKQKSV 60

Query: 61  PDISKIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFN 120
            +  +IQR+ I+NKQRS+SPLP++ LS+ FKEA+SE +RFSTP P+ +E  K F+ K F+
Sbjct: 61  HNDPQIQRNPITNKQRSVSPLPQTILSDTFKEARSERRRFSTPNPQRKESDKRFRGKFFH 120

Query: 121 KDPQDSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFD--SGKVTAVETADDW 180
           K+  D K SNS +S N+SPL+ L S K  D+SK +K+S+WTKYFD   G+V AVE AD+ 
Sbjct: 121 KESHDKKGSNSPASSNSSPLRHLGSIKVQDRSKSRKESAWTKYFDHAGGRVNAVEAADEH 180

Query: 181 TVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVT 240
           TV+LS+LF+GLRFAHGAHSRLYHG Y +EPVAVKIIRVPDDDENG LAARLEKQF REVT
Sbjct: 181 TVELSQLFLGLRFAHGAHSRLYHGIYKEEPVAVKIIRVPDDDENGNLAARLEKQFNREVT 240

Query: 241 LLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVA 300
           LLSRL+HPNVIKFVAACR PPV+CVITEYLS+GSLRAYLHKL+H+SLP++KLI   L+VA
Sbjct: 241 LLSRLHHPNVIKFVAACRKPPVFCVITEYLSEGSLRAYLHKLDHKSLPMEKLIAIVLEVA 300

Query: 301 RGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEM 360
           RGMEY+HSQGVIHRDLKPENVLID++ HLKIADFGIAC EA+ D LADDPGTYRWMAPEM
Sbjct: 301 RGMEYIHSQGVIHRDLKPENVLIDQEFHLKIADFGIACEEAYCDLLADDPGTYRWMAPEM 360

Query: 361 IKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRA 420
           IK K   RKVDVYSFGL+LWEMV+G IPYEDM PIQAAFAVVNKNLRPVI  +CP AMRA
Sbjct: 361 IKKKSYGRKVDVYSFGLILWEMVAGTIPYEDMNPIQAAFAVVNKNLRPVIPHNCPPAMRA 420

Query: 421 LIEQCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKL 480
           LIEQCWSL P+KRPDFWQIVKVLEQFESSL +DGTL L+ NP  S  DHKKGLLHWIQKL
Sbjct: 421 LIEQCWSLHPEKRPDFWQIVKVLEQFESSLNQDGTLKLVQNP--SCQDHKKGLLHWIQKL 480

Query: 481 GPLHPEVS--SSPVPKPKFS 493
           GP+HP  +  SSP+PKPKF+
Sbjct: 481 GPVHPHSNAYSSPMPKPKFT 498

BLAST of Cucsa.046250 vs. TrEMBL
Match: A5BU69_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_022074 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 7.6e-199
Identity = 343/492 (69.72%), Postives = 400/492 (81.30%), Query Frame = 1

Query: 3   EEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDISKI 62
           E+ANSW+RRTKFSHT++HR DS +L   P  + P RNSGLKSRP   S   K  P   + 
Sbjct: 2   EDANSWIRRTKFSHTIFHRLDSTRLASFPLTISPERNSGLKSRPQRGSLNLKSEPSDPQG 61

Query: 63  QRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQDS 122
           +R+  +NKQRSLSPLPES LS+ FKEA+S+ KRFSTP PR +E  K    KLF+KD QD 
Sbjct: 62  RRNAFTNKQRSLSPLPESKLSDEFKEARSDRKRFSTPVPRRKEHEKGIMGKLFSKDSQDP 121

Query: 123 KSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFD--SGKVTAVETADDWTVDLSK 182
           K+SNS    NTSPL+ L+S K  DK+K +K+ SW KYFD   G+VTAVE AD+W  DLSK
Sbjct: 122 KASNSNKQSNTSPLRHLTSTKNHDKAKFRKEPSWIKYFDHGGGRVTAVEAADEWMADLSK 181

Query: 183 LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLY 242
           L++GLRF+HGAHSRLYHG Y ++PVAVK+I VPD++ENG LA++LEKQF  EV+ LSRL+
Sbjct: 182 LYLGLRFSHGAHSRLYHGVYEEQPVAVKVIMVPDEEENGALASKLEKQFNGEVSCLSRLH 241

Query: 243 HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL 302
           H NVIKFVAA R PPV+ VITEYLS+GSLRAYLHKLEH+SLPL+KLI  ALD+ARGMEY+
Sbjct: 242 HQNVIKFVAAWRRPPVFVVITEYLSEGSLRAYLHKLEHKSLPLEKLITIALDIARGMEYI 301

Query: 303 HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC 362
           HSQG+IHRDLKPENVL+ +D H+KIADFGIAC EA+ D LADDPGTYRWMAPEMIKHK  
Sbjct: 302 HSQGIIHRDLKPENVLVTKDFHMKIADFGIACEEAYCDSLADDPGTYRWMAPEMIKHKSY 361

Query: 363 SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW 422
            RKVDVYSFGL+LWEMV+G IPYEDM PIQAAFAVVNKNLRPVI  DCP AMRALIEQCW
Sbjct: 362 GRKVDVYSFGLILWEMVAGTIPYEDMAPIQAAFAVVNKNLRPVIPVDCPPAMRALIEQCW 421

Query: 423 SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPE 482
           SL PDKRP+FWQ+VKVLEQF+SSLA DGTLNL+    ++  DHKKGLLHWIQKLGP+H  
Sbjct: 422 SLHPDKRPEFWQVVKVLEQFKSSLALDGTLNLV--RYATCQDHKKGLLHWIQKLGPVHHN 481

Query: 483 VSSSPVPKPKFS 493
            SS  +PKPKF+
Sbjct: 482 TSS--MPKPKFT 489

BLAST of Cucsa.046250 vs. TrEMBL
Match: A0A067JN83_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21450 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 9.9e-199
Identity = 353/501 (70.46%), Postives = 408/501 (81.44%), Query Frame = 1

Query: 3   EEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQK----PNPD 62
           EE +SW+RRTKFSHTV HR DS +L   P  +   RN+GLKSRP  +S  QK    P   
Sbjct: 2   EEDSSWIRRTKFSHTVCHRLDSSRLVSFPLSIPSERNAGLKSRPGKSSPYQKSVSFPTQR 61

Query: 63  IS-----KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNK 122
            S     +IQR+ I+NKQRSLSPLPE+ LS+ FKEA+ + KRFSTP PR ++  K    K
Sbjct: 62  SSVTSDFQIQRNPITNKQRSLSPLPETTLSDTFKEARFDRKRFSTPHPRRKDHDKGIMGK 121

Query: 123 LFNKDPQDSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFD--SGKVTAVETA 182
            F+K+  ++KS+N+ S  N+SPL  L   KG +KSK KKDS+WTKYFD   G+V AVE A
Sbjct: 122 FFHKESSETKSTNTSS--NSSPLLHL---KGHEKSKGKKDSAWTKYFDHAGGRVNAVEAA 181

Query: 183 DDWTVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTR 242
           D+++VD+S+LF+GLRFAHGAHSRLYHG Y DEPVAVKIIR PDDDENGTLA RL+ Q+ R
Sbjct: 182 DEFSVDMSQLFLGLRFAHGAHSRLYHGVYKDEPVAVKIIRAPDDDENGTLALRLKNQYDR 241

Query: 243 EVTLLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFAL 302
           EVTLLSRL+HPNVI+FVAAC+ P VYCVITEYLS+GSLRAYLHKLEH+ LPL KLI  AL
Sbjct: 242 EVTLLSRLHHPNVIRFVAACKKPAVYCVITEYLSEGSLRAYLHKLEHKLLPLPKLIAIAL 301

Query: 303 DVARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMA 362
           D+ARGMEY+HSQGVIHRDLKPENVLID++ HLKIADFGIAC EA+ D LADDPGTYRWMA
Sbjct: 302 DIARGMEYIHSQGVIHRDLKPENVLIDQEFHLKIADFGIACEEAYCDALADDPGTYRWMA 361

Query: 363 PEMIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 422
           PEMIK K   R+VDVYSFGL+LWEMV+G IPYEDM PIQAAFAVVNKNLRPV+  DCP A
Sbjct: 362 PEMIKKKSYGRRVDVYSFGLILWEMVAGTIPYEDMNPIQAAFAVVNKNLRPVVPRDCPPA 421

Query: 423 MRALIEQCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWI 482
           MRALIEQCWSLQP+KRP+FWQIVKVLE+FESSLARDGTLNL+ NP  +F DHKKGLLHWI
Sbjct: 422 MRALIEQCWSLQPEKRPEFWQIVKVLEEFESSLARDGTLNLVQNP--TFQDHKKGLLHWI 481

Query: 483 QKLGPLHPEVSSSPVPKPKFS 493
           QKLGP+HP  S +P PKPKF+
Sbjct: 482 QKLGPVHPSGSPTPTPKPKFT 495

BLAST of Cucsa.046250 vs. TAIR10
Match: AT5G58950.1 (AT5G58950.1 Protein kinase superfamily protein)

HSP 1 Score: 604.4 bits (1557), Expect = 6.4e-173
Identity = 310/529 (58.60%), Postives = 392/529 (74.10%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPP---SASSAQKPNP 60
           MDEEA SW+RR KFS TV +R +S KL  +PF++   + SGLK+ P    S+SSA   +P
Sbjct: 1   MDEEATSWIRRAKFSQTVSYRLNSSKLASLPFMINQDKFSGLKAIPQRSSSSSSASSSDP 60

Query: 61  DI-----------------------SKIQRSFISNKQRSLSPLPESNLSEVFKEAKSESK 120
            +                       S+IQ + +++KQRS+SP P+  + +VFKEA+SE K
Sbjct: 61  KLVSSNSQTTGDTSSLEAADVYVVDSEIQTNPVTHKQRSVSPSPQMAVPDVFKEARSERK 120

Query: 121 RFSTPTPRLRERTKEFKNKLFNKDPQDSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDS 180
           RFSTP PR  E  K  K KL +K+  D +S N +S   + P++ L + +  ++ K KKD+
Sbjct: 121 RFSTPHPRRVESEKGMKPKLSHKNSFDKRSFNLRSP--SGPIRDLGTLRIQERVKSKKDT 180

Query: 181 SWTKYFDSG--KVTAVETADDWTVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRV 240
            W+K FD+   +V+AVE ++++ VD+SKLF GL+FAHG +SRLYHGKY D+ VAVK+I V
Sbjct: 181 GWSKLFDNTGRRVSAVEASEEFRVDMSKLFFGLKFAHGLYSRLYHGKYEDKAVAVKLITV 240

Query: 241 PDDDENGTLAARLEKQFTREVTLLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAY 300
           PDDD+NG L ARLEKQFT+EVTLLSRL HPNVIKFV A ++PPVYCV+T+YL +GSLR++
Sbjct: 241 PDDDDNGCLGARLEKQFTKEVTLLSRLTHPNVIKFVGAYKDPPVYCVLTQYLPEGSLRSF 300

Query: 301 LHKLEHQSLPLQKLIKFALDVARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIAC 360
           LHK E++SLPL+KLI+FA+D+ARGMEY+HS+ +IHRDLKPENVLIDE+ HLKIADFGIAC
Sbjct: 301 LHKPENRSLPLKKLIEFAIDIARGMEYIHSRRIIHRDLKPENVLIDEEFHLKIADFGIAC 360

Query: 361 PEAFFDPLADDPGTYRWMAPEMIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAA 420
            E + D LADDPGTYRWMAPEMIK KP  RK DVYSFGL+LWEMV+GAIPYEDM PIQAA
Sbjct: 361 EEEYCDMLADDPGTYRWMAPEMIKRKPHGRKADVYSFGLVLWEMVAGAIPYEDMNPIQAA 420

Query: 421 FAVVNKNLRPVISSDCPLAMRALIEQCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNL 480
           FAVV+KN+RP I  DCP+AM+ALIEQCWS+ PDKRP+FWQIVKVLEQF  SL R+G LNL
Sbjct: 421 FAVVHKNIRPAIPGDCPVAMKALIEQCWSVAPDKRPEFWQIVKVLEQFAISLEREGNLNL 480

Query: 481 LGNPLSSFHDHKKGLLHWIQKLGPLH---------PEVSSSPVPKPKFS 493
             + +    D +KGL HWIQKLGP+H           +  S +PKPKF+
Sbjct: 481 SSSKICK--DPRKGLKHWIQKLGPVHAGGGGGSSSSGLGGSALPKPKFA 525

BLAST of Cucsa.046250 vs. TAIR10
Match: AT3G46930.1 (AT3G46930.1 Protein kinase superfamily protein)

HSP 1 Score: 463.4 bits (1191), Expect = 1.8e-130
Identity = 253/503 (50.30%), Postives = 329/503 (65.41%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS 60
           MD E  SW+RR  FSHTV +R  +  L  +PF V                          
Sbjct: 1   MDGEVTSWIRRANFSHTVCYRMITPSLESMPFTVNQ-----------------------E 60

Query: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFN-KDP 120
           K+QR+ ++NK+RS+SPLP   LS+ F EAKS+ KRFSTP PR  E  K  K K  + KD 
Sbjct: 61  KMQRNPVTNKKRSVSPLPHMALSDAFIEAKSDIKRFSTPHPRRVEPEKGMKAKSSSRKDS 120

Query: 121 QDSKSSNSKSSLNTSPLKQLSSGKGGD--KSKLKKDSSWTKYFDSGKVTAVETADDWTVD 180
            + KS N +S  ++ P++ LS+ K  +  KSK+ K SS +  +   KV++    ++  +D
Sbjct: 121 SEKKSVNLRSLSHSGPIRDLSTQKVKERGKSKIDKKSSKSVDYRGSKVSSAGVLEECLID 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLS 240
           +SKL  G RFAHG +S++YHG+Y  + VA+KII  P+D ++  L ARLEK+F  E TLLS
Sbjct: 181 VSKLSYGDRFAHGKYSQIYHGEYEGKAVALKIITAPEDSDDIFLGARLEKEFIVEATLLS 240

Query: 241 RLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGM 300
           RL HPNV+KFV         C+ITEY+ +GSLR+YLHKLE +SLPL++LI F LD+A+GM
Sbjct: 241 RLSHPNVVKFVGVNTGN---CIITEYVPRGSLRSYLHKLEQKSLPLEQLIDFGLDIAKGM 300

Query: 301 EYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EY+HS+ ++H+DLKPENVLID D HLKIADFGIAC E + D L D+ GTYRWMAPE++K 
Sbjct: 301 EYIHSREIVHQDLKPENVLIDNDFHLKIADFGIACEEEYCDVLGDNIGTYRWMAPEVLKR 360

Query: 361 KPCSRKVDVYSFGLMLWEMVSGAIPYEDMT-PIQAAFAVVNKNLRPVISSDCPLAMRALI 420
            P  RK DVYSFGL+LWEMV+GA+PYE+M    Q A+AV+ K +RPVI +DCP AM+ LI
Sbjct: 361 IPHGRKCDVYSFGLLLWEMVAGALPYEEMKFAEQIAYAVIYKKIRPVIPTDCPAAMKELI 420

Query: 421 EQCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGP 480
           E+CWS Q DKRP+FWQIVKVLE F+ SL  +G LNLL  P     + KK    WI   G 
Sbjct: 421 ERCWSSQTDKRPEFWQIVKVLEHFKKSLTSEGKLNLL--PSQICPELKKCPKFWIHIFGS 475

Query: 481 LHPEVS-------SSPVPKPKFS 493
            H   S       +S +PKPKF+
Sbjct: 481 FHHHSSGGGSSSNNSALPKPKFA 475

BLAST of Cucsa.046250 vs. TAIR10
Match: AT1G62400.1 (AT1G62400.1 Protein kinase superfamily protein)

HSP 1 Score: 339.0 bits (868), Expect = 5.0e-93
Identity = 170/349 (48.71%), Postives = 232/349 (66.48%), Query Frame = 1

Query: 146 DKSKLKKDSSWTKYFDSGKVTAVETA----DDWTVDLSKLFVGLRFAHGAHSRLYHGKYN 205
           +K +     SW+   +S  V   E +    ++WT DLS+LF+G +FA GAHSR+Y G Y 
Sbjct: 2   EKKRFDSMESWSMILESENVETWEASKGEREEWTADLSQLFIGNKFASGAHSRIYRGIYK 61

Query: 206 DEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLYHPNVIKFVAACRNPPVYCVIT 265
              VAVK++R+P   E     A+LE+QF  EV LLSRL+HPN+++F+AAC+ PPVYC+IT
Sbjct: 62  QRAVAVKMVRIPTHKEE--TRAKLEQQFKSEVALLSRLFHPNIVQFIAACKKPPVYCIIT 121

Query: 266 EYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYLHSQGVIHRDLKPENVLIDEDM 325
           EY+SQG+LR YL+K E  SL ++ +++ ALD++RGMEYLHSQGVIHRDLK  N+L++++M
Sbjct: 122 EYMSQGNLRMYLNKKEPYSLSIETVLRLALDISRGMEYLHSQGVIHRDLKSNNLLLNDEM 181

Query: 326 HLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPCSRKVDVYSFGLMLWEMVSGAI 385
            +K+ADFG +C E        + GTYRWMAPEMIK KP +RKVDVYSFG++LWE+ +  +
Sbjct: 182 RVKVADFGTSCLETQCREAKGNMGTYRWMAPEMIKEKPYTRKVDVYSFGIVLWELTTALL 241

Query: 386 PYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCWSLQPDKRPDFWQIVKVLEQFE 445
           P++ MTP+QAAFAV  KN RP + + C  A+  LI++CWS  P KRPDF  IV VLE+++
Sbjct: 242 PFQGMTPVQAAFAVAEKNERPPLPASCQPALAHLIKRCWSENPSKRPDFSNIVAVLEKYD 301

Query: 446 SSLARDGTLN---LLGNPLSSFHDHKKGLLHWIQKLGPLHPEVSSSPVP 488
             +     L     L     +  DH KG +  I          SSS VP
Sbjct: 302 ECVKEGLPLTSHASLTKTKKAILDHLKGCVTSISS------PFSSSSVP 342

BLAST of Cucsa.046250 vs. TAIR10
Match: AT2G24360.1 (AT2G24360.1 Protein kinase superfamily protein)

HSP 1 Score: 287.3 bits (734), Expect = 1.7e-77
Identity = 129/273 (47.25%), Postives = 197/273 (72.16%), Query Frame = 1

Query: 172 DDWTVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTR 231
           D+WT+DL KL +G  FA GA  +LY G YN E VA+KI+  P++      A  +E+QF +
Sbjct: 121 DEWTIDLRKLNMGPAFAQGAFGKLYKGTYNGEDVAIKILERPENSPEK--AQFMEQQFQQ 180

Query: 232 EVTLLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFAL 291
           EV++L+ L HPN+++F+ ACR P V+C++TEY   GS+R +L + +++++PL+  +K AL
Sbjct: 181 EVSMLANLKHPNIVRFIGACRKPMVWCIVTEYAKGGSVRQFLTRRQNRAVPLKLAVKQAL 240

Query: 292 DVARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMA 351
           DVARGM Y+H +  IHRDLK +N+LI  D  +KIADFG+A  E   + +  + GTYRWMA
Sbjct: 241 DVARGMAYVHGRNFIHRDLKSDNLLISADKSIKIADFGVARIEVQTEGMTPETGTYRWMA 300

Query: 352 PEMIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 411
           PEMI+H+  ++KVDVYSFG++LWE+++G +P+++MT +QAAFAVVN+ +RP + +DC   
Sbjct: 301 PEMIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPNDCLPV 360

Query: 412 MRALIEQCWSLQPDKRPDFWQIVKVLEQFESSL 445
           +  ++ +CW   P+ RP F ++VK+LE  E+ +
Sbjct: 361 LSDIMTRCWDANPEVRPCFVEVVKLLEAAETEI 391

BLAST of Cucsa.046250 vs. TAIR10
Match: AT4G31170.1 (AT4G31170.1 Protein kinase superfamily protein)

HSP 1 Score: 284.3 bits (726), Expect = 1.5e-76
Identity = 129/273 (47.25%), Postives = 194/273 (71.06%), Query Frame = 1

Query: 172 DDWTVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTR 231
           ++WT+DL KL +G  FA GA  +LY G YN E VA+K++     D N   A  LE+QF +
Sbjct: 122 EEWTIDLRKLHMGPAFAQGAFGKLYRGTYNGEDVAIKLLE--RSDSNPEKAQALEQQFQQ 181

Query: 232 EVTLLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFAL 291
           EV++L+ L HPN+++F+ AC  P V+C++TEY   GS+R +L K +++++PL+  +  AL
Sbjct: 182 EVSMLAFLKHPNIVRFIGACIKPMVWCIVTEYAKGGSVRQFLTKRQNRAVPLKLAVMQAL 241

Query: 292 DVARGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMA 351
           DVARGM Y+H +  IHRDLK +N+LI  D  +KIADFG+A  E   + +  + GTYRWMA
Sbjct: 242 DVARGMAYVHERNFIHRDLKSDNLLISADRSIKIADFGVARIEVQTEGMTPETGTYRWMA 301

Query: 352 PEMIKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 411
           PEMI+H+P ++KVDVYSFG++LWE+++G +P+++MT +QAAFAVVN+ +RP + +DC   
Sbjct: 302 PEMIQHRPYTQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPADCLPV 361

Query: 412 MRALIEQCWSLQPDKRPDFWQIVKVLEQFESSL 445
           +  ++ +CW   P+ RP F +IV +LE  E+ +
Sbjct: 362 LGEIMTRCWDADPEVRPCFAEIVNLLEAAETEI 392

BLAST of Cucsa.046250 vs. NCBI nr
Match: gi|449433834|ref|XP_004134702.1| (PREDICTED: serine/threonine-protein kinase HT1 [Cucumis sativus])

HSP 1 Score: 1005.0 bits (2597), Expect = 4.6e-290
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS 60
           MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS 60

Query: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ 120
           KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ
Sbjct: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ 120

Query: 121 DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSK 180
           DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSK
Sbjct: 121 DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSK 180

Query: 181 LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLY 240
           LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLY
Sbjct: 181 LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLY 240

Query: 241 HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL 300
           HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL
Sbjct: 241 HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL 300

Query: 301 HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC 360
           HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC
Sbjct: 301 HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC 360

Query: 361 SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW 420
           SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW
Sbjct: 361 SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW 420

Query: 421 SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPE 480
           SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPE
Sbjct: 421 SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPE 480

Query: 481 VSSSPVPKPKFS 493
           VSSSPVPKPKFS
Sbjct: 481 VSSSPVPKPKFS 492

BLAST of Cucsa.046250 vs. NCBI nr
Match: gi|659078675|ref|XP_008439850.1| (PREDICTED: serine/threonine-protein kinase HT1 [Cucumis melo])

HSP 1 Score: 976.5 bits (2523), Expect = 1.8e-281
Identity = 479/492 (97.36%), Postives = 485/492 (98.58%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS 60
           MDEEANSWLRRTKFSHTVYHRWDSL+LNPVPFIVEPPRNSGLKSRP SASSAQKPNPDIS
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPRNSGLKSRPSSASSAQKPNPDIS 60

Query: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ 120
           KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLR RTKEFKNKLFNKDPQ
Sbjct: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRGRTKEFKNKLFNKDPQ 120

Query: 121 DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDSGKVTAVETADDWTVDLSK 180
           DSKS+NSK SLNTSPLKQLSSGKGGDKSKLK DSSWTKYFDSGKVTAVE+ADDWTVDLSK
Sbjct: 121 DSKSANSKPSLNTSPLKQLSSGKGGDKSKLK-DSSWTKYFDSGKVTAVESADDWTVDLSK 180

Query: 181 LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSRLY 240
           LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLA+RLEKQFTREVTLLSRL+
Sbjct: 181 LFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLASRLEKQFTREVTLLSRLH 240

Query: 241 HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL 300
           HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL
Sbjct: 241 HPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGMEYL 300

Query: 301 HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC 360
           HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC
Sbjct: 301 HSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPC 360

Query: 361 SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW 420
           SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW
Sbjct: 361 SRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCW 420

Query: 421 SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLHPE 480
           SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFH+HKKGLLHWIQKLGPLHPE
Sbjct: 421 SLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHEHKKGLLHWIQKLGPLHPE 480

Query: 481 VSSSPVPKPKFS 493
            S  PVPKPKFS
Sbjct: 481 TSPLPVPKPKFS 491

BLAST of Cucsa.046250 vs. NCBI nr
Match: gi|10241603|emb|CAC09580.1| (protein kinase (PK), partial [Fagus sylvatica])

HSP 1 Score: 712.2 bits (1837), Expect = 6.2e-202
Identity = 358/494 (72.47%), Postives = 405/494 (81.98%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPSASSAQKPNPDIS 60
           M EE NSW+RRTKFSHTV HR D+ +L   P I+ P RNSGLKSRP + SS++K      
Sbjct: 1   MGEEGNSWIRRTKFSHTVCHRLDASRLASFPIIIRPERNSGLKSRPGATSSSEKFGT--- 60

Query: 61  KIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDPQ 120
                 I NKQRSLSPLPES LS+VFKEA+SE+KRF TP PR RE+ K    K  +KD  
Sbjct: 61  ------IYNKQRSLSPLPESRLSDVFKEARSETKRFMTPLPRRREQAKGVMGKFVHKDFH 120

Query: 121 DSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFD--SGKVTAVETADDWTVDL 180
           + K+S+SKS  N+S L+ LSS K  DKSK +K+SSW KYFD   G+V AVET+D+WTVDL
Sbjct: 121 EVKASDSKSLANSSALRHLSSMKVNDKSKYRKESSWAKYFDHGGGRVNAVETSDEWTVDL 180

Query: 181 SKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLSR 240
           SKLF+GL+FAHGAHSRLYHG YNDEPVAVKIIRVP+DDENG L ARLEKQF REVTLLSR
Sbjct: 181 SKLFLGLKFAHGAHSRLYHGIYNDEPVAVKIIRVPEDDENGALGARLEKQFNREVTLLSR 240

Query: 241 LYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGME 300
           L+  N+IKFVAACR PPVYCV+TEYLS+GSLRAYLHKLE +SLPLQKLI FALD+ARGME
Sbjct: 241 LHFHNIIKFVAACRKPPVYCVVTEYLSEGSLRAYLHKLERKSLPLQKLIAFALDIARGME 300

Query: 301 YLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHK 360
           Y+HSQGVIHRDLKPENVLID++ HLKIADFGIAC EA+ D LADDPGTYRWMAPEMIKHK
Sbjct: 301 YIHSQGVIHRDLKPENVLIDQEFHLKIADFGIACEEAYCDSLADDPGTYRWMAPEMIKHK 360

Query: 361 PCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQ 420
              RKVDVYSFGL+LWEMV+G IPYEDM P+QAAFAVVNKNLRPVI   CP AMRALIEQ
Sbjct: 361 SYGRKVDVYSFGLILWEMVAGTIPYEDMNPVQAAFAVVNKNLRPVIPRYCPPAMRALIEQ 420

Query: 421 CWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGPLH 480
           CWSLQ +KRP+FWQ+VKVLEQFESSLARDGTLNL+ +   +  DHKKGLLHWI KLGP+H
Sbjct: 421 CWSLQSEKRPEFWQVVKVLEQFESSLARDGTLNLVQS--LTCQDHKKGLLHWIHKLGPVH 480

Query: 481 PEVSSSPVPKPKFS 493
           P  S   +PKPK +
Sbjct: 481 PNGS---MPKPKLT 480

BLAST of Cucsa.046250 vs. NCBI nr
Match: gi|590624433|ref|XP_007025602.1| (Tyrosine kinase, putative [Theobroma cacao])

HSP 1 Score: 709.9 bits (1831), Expect = 3.1e-201
Identity = 356/500 (71.20%), Postives = 413/500 (82.60%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNS----GLKSRPPSASSAQKPN 60
           M ++ANSW+RRTKFSHTV HR DS +L   PF ++  RN     GLK R   AS+ QK  
Sbjct: 1   MADDANSWIRRTKFSHTVCHRLDSSRLVSFPFHLQSDRNLEWSLGLKPRSAVASAKQKSV 60

Query: 61  PDISKIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFN 120
            +  +IQR+ I+NKQRS+SPLP++ LS+ FKEA+SE +RFSTP P+ +E  K F+ K F+
Sbjct: 61  HNDPQIQRNPITNKQRSVSPLPQTILSDTFKEARSERRRFSTPNPQRKESDKRFRGKFFH 120

Query: 121 KDPQDSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFD--SGKVTAVETADDW 180
           K+  D K SNS +S N+SPL+ L S K  D+SK +K+S+WTKYFD   G+V AVE AD+ 
Sbjct: 121 KESHDKKGSNSPASSNSSPLRHLGSIKVQDRSKSRKESAWTKYFDHAGGRVNAVEAADEH 180

Query: 181 TVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVT 240
           TV+LS+LF+GLRFAHGAHSRLYHG Y +EPVAVKIIRVPDDDENG LAARLEKQF REVT
Sbjct: 181 TVELSQLFLGLRFAHGAHSRLYHGIYKEEPVAVKIIRVPDDDENGNLAARLEKQFNREVT 240

Query: 241 LLSRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVA 300
           LLSRL+HPNVIKFVAACR PPV+CVITEYLS+GSLRAYLHKL+H+SLP++KLI   L+VA
Sbjct: 241 LLSRLHHPNVIKFVAACRKPPVFCVITEYLSEGSLRAYLHKLDHKSLPMEKLIAIVLEVA 300

Query: 301 RGMEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEM 360
           RGMEY+HSQGVIHRDLKPENVLID++ HLKIADFGIAC EA+ D LADDPGTYRWMAPEM
Sbjct: 301 RGMEYIHSQGVIHRDLKPENVLIDQEFHLKIADFGIACEEAYCDLLADDPGTYRWMAPEM 360

Query: 361 IKHKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRA 420
           IK K   RKVDVYSFGL+LWEMV+G IPYEDM PIQAAFAVVNKNLRPVI  +CP AMRA
Sbjct: 361 IKKKSYGRKVDVYSFGLILWEMVAGTIPYEDMNPIQAAFAVVNKNLRPVIPHNCPPAMRA 420

Query: 421 LIEQCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKL 480
           LIEQCWSL P+KRPDFWQIVKVLEQFESSL +DGTL L+ NP  S  DHKKGLLHWIQKL
Sbjct: 421 LIEQCWSLHPEKRPDFWQIVKVLEQFESSLNQDGTLKLVQNP--SCQDHKKGLLHWIQKL 480

Query: 481 GPLHPEVS--SSPVPKPKFS 493
           GP+HP  +  SSP+PKPKF+
Sbjct: 481 GPVHPHSNAYSSPMPKPKFT 498

BLAST of Cucsa.046250 vs. NCBI nr
Match: gi|1009168151|ref|XP_015902501.1| (PREDICTED: serine/threonine-protein kinase HT1-like [Ziziphus jujuba])

HSP 1 Score: 706.1 bits (1821), Expect = 4.4e-200
Identity = 355/495 (71.72%), Postives = 409/495 (82.63%), Query Frame = 1

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPPRNSGLKSRPPS--ASSAQKPNPD 60
           M+ E +SW+RRTKFSHTV HRWDS +L  VPF V+  R SGLKSRPP+  AS   K NP 
Sbjct: 1   MEGEVSSWIRRTKFSHTVCHRWDSSRLASVPFNVQIDRISGLKSRPPNREASGNHKENPS 60

Query: 61  ISKIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKD 120
            S+IQR+ ++NKQRSLSPLPE+ LSE FKEA+S+ KRFSTP  R +E  K     +F+KD
Sbjct: 61  YSQIQRNPVTNKQRSLSPLPETLLSEEFKEARSDWKRFSTPNRRRKEIDKGIVGNVFHKD 120

Query: 121 PQDSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFD--SGKVTAVETADDWTV 180
            QDS+ S + +S +TSPL+ L+S K  DKSK +++S W KYFD  +GKVTAVE AD+W+V
Sbjct: 121 SQDSQVSKALNS-STSPLRHLASMKVNDKSKNRRESPWAKYFDHAAGKVTAVEAADEWSV 180

Query: 181 DLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLL 240
           D+SKLF+GL+FAHGAHSRLYHG Y DEPVAVKIIRVPDDDE+G LAARLEKQF+REV LL
Sbjct: 181 DMSKLFLGLKFAHGAHSRLYHGIYFDEPVAVKIIRVPDDDESGALAARLEKQFSREVNLL 240

Query: 241 SRLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARG 300
           SRL+H NVIKF AAC  PPVYCVITEYLS+GSLRAYLHKLEH+SLPLQKLI  ALD+ARG
Sbjct: 241 SRLHHQNVIKFKAACGKPPVYCVITEYLSEGSLRAYLHKLEHKSLPLQKLIAIALDIARG 300

Query: 301 MEYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIK 360
           ME++HSQ VIHRDLKPEN+LID+D  LK+ADFGIAC E + D LADDPGTYRWMAPE+IK
Sbjct: 301 MEFIHSQHVIHRDLKPENILIDQDFRLKVADFGIACEETYCDSLADDPGTYRWMAPELIK 360

Query: 361 HKPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALI 420
           HK   RKVDVYSFGL+LWEMV+G IP+EDM PIQAAFAVVNKNLRP+I  DCP AMRALI
Sbjct: 361 HKCYGRKVDVYSFGLILWEMVAGTIPFEDMNPIQAAFAVVNKNLRPIIPGDCPPAMRALI 420

Query: 421 EQCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGP 480
           EQCWSLQPDKRP+FWQIVKVLEQF +SLA DGTL+L+ N   + HDHKK LL WIQKLGP
Sbjct: 421 EQCWSLQPDKRPEFWQIVKVLEQFVTSLAYDGTLSLVLN--QTCHDHKKQLLRWIQKLGP 480

Query: 481 LHPEVSSSPVPKPKF 492
           +H   SS P PKPKF
Sbjct: 481 VHSTSSSMPKPKPKF 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HT1_ARATH1.4e-9245.93Serine/threonine-protein kinase HT1 OS=Arabidopsis thaliana GN=HT1 PE=1 SV=1[more]
STY46_ARATH1.6e-6140.79Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana GN=STY46 PE=1 SV=1[more]
STY8_ARATH4.8e-6138.49Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana GN=STY8 PE=1 SV=2[more]
STY17_ARATH9.0e-6035.33Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana GN=STY17 PE=1 SV=1[more]
Y9955_DICDI3.4e-5135.05Probable serine/threonine-protein kinase DDB_G0267514 OS=Dictyostelium discoideu... [more]
Match NameE-valueIdentityDescription
A0A0A0KNC4_CUCSA3.2e-290100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G517390 PE=4 SV=1[more]
Q9FDV7_FAGSY4.3e-20272.47Protein kinase (PK) (Fragment) OS=Fagus sylvatica GN=pk1 PE=2 SV=1[more]
A0A061GMB7_THECC2.1e-20171.20Tyrosine kinase, putative OS=Theobroma cacao GN=TCM_029853 PE=4 SV=1[more]
A5BU69_VITVI7.6e-19969.72Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_022074 PE=4 SV=1[more]
A0A067JN83_JATCU9.9e-19970.46Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21450 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G58950.16.4e-17358.60 Protein kinase superfamily protein[more]
AT3G46930.11.8e-13050.30 Protein kinase superfamily protein[more]
AT1G62400.15.0e-9348.71 Protein kinase superfamily protein[more]
AT2G24360.11.7e-7747.25 Protein kinase superfamily protein[more]
AT4G31170.11.5e-7647.25 Protein kinase superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449433834|ref|XP_004134702.1|4.6e-290100.00PREDICTED: serine/threonine-protein kinase HT1 [Cucumis sativus][more]
gi|659078675|ref|XP_008439850.1|1.8e-28197.36PREDICTED: serine/threonine-protein kinase HT1 [Cucumis melo][more]
gi|10241603|emb|CAC09580.1|6.2e-20272.47protein kinase (PK), partial [Fagus sylvatica][more]
gi|590624433|ref|XP_007025602.1|3.1e-20171.20Tyrosine kinase, putative [Theobroma cacao][more]
gi|1009168151|ref|XP_015902501.1|4.4e-20071.72PREDICTED: serine/threonine-protein kinase HT1-like [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000719Prot_kinase_dom
IPR001245Ser-Thr/Tyr_kinase_cat_dom
IPR008271Ser/Thr_kinase_AS
IPR011009Kinase-like_dom_sf
IPR000719Prot_kinase_dom
IPR001245Ser-Thr/Tyr_kinase_cat_dom
IPR008271Ser/Thr_kinase_AS
IPR011009Kinase-like_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
GO:0006468protein phosphorylation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000165 MAPK cascade
biological_process GO:0018108 peptidyl-tyrosine phosphorylation
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004713 protein tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.046250.2Cucsa.046250.2mRNA
Cucsa.046250.1Cucsa.046250.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 181..440
score: 3.1
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 181..441
score: 41
IPR001245Serine-threonine/tyrosine-protein kinase catalytic domainPRINTSPR00109TYRKINASEcoord: 364..386
score: 4.3E-19coord: 408..430
score: 4.3E-19coord: 299..317
score: 4.3E-19coord: 345..355
score: 4.3E-19coord: 261..274
score: 4.3
IPR001245Serine-threonine/tyrosine-protein kinase catalytic domainPFAMPF07714Pkinase_Tyrcoord: 188..437
score: 3.0
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 305..317
scor
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 166..437
score: 4.05
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 250..435
score: 4.9
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 172..249
score: 3.7
NoneNo IPR availablePANTHERPTHR23257SERINE-THREONINE PROTEIN KINASEcoord: 58..488
score: 1.3E
NoneNo IPR availablePANTHERPTHR23257:SF81SUBFAMILY NOT NAMEDcoord: 58..488
score: 1.3E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.046250Cla009622Watermelon (97103) v1cgywmB026
Cucsa.046250Cla019713Watermelon (97103) v1cgywmB022
Cucsa.046250Csa1G074900Cucumber (Chinese Long) v2cgycuB015
Cucsa.046250Csa6G517390Cucumber (Chinese Long) v2cgycuB027
Cucsa.046250MELO3C007280Melon (DHL92) v3.5.1cgymeB027
Cucsa.046250MELO3C002609Melon (DHL92) v3.5.1cgymeB019
Cucsa.046250ClCG03G008190Watermelon (Charleston Gray)cgywcgB027
Cucsa.046250ClCG01G023030Watermelon (Charleston Gray)cgywcgB022
Cucsa.046250CSPI01G12170Wild cucumber (PI 183967)cgycpiB016
Cucsa.046250CSPI06G34660Wild cucumber (PI 183967)cgycpiB028
Cucsa.046250CmaCh13G006400Cucurbita maxima (Rimu)cgycmaB0035
Cucsa.046250CmaCh18G003740Cucurbita maxima (Rimu)cgycmaB0037
Cucsa.046250CmaCh03G012670Cucurbita maxima (Rimu)cgycmaB0045
Cucsa.046250CmaCh07G002400Cucurbita maxima (Rimu)cgycmaB0048
Cucsa.046250CmoCh03G012620Cucurbita moschata (Rifu)cgycmoB0040
Cucsa.046250CmoCh18G003540Cucurbita moschata (Rifu)cgycmoB0034
Cucsa.046250CmoCh13G006590Cucurbita moschata (Rifu)cgycmoB0033
Cucsa.046250Lsi01G003110Bottle gourd (USVL1VR-Ls)cgylsiB022
Cucsa.046250Lsi02G007590Bottle gourd (USVL1VR-Ls)cgylsiB023
Cucsa.046250Cp4.1LG10g04960Cucurbita pepo (Zucchini)cgycpeB0034
Cucsa.046250Cp4.1LG09g08300Cucurbita pepo (Zucchini)cgycpeB0032
Cucsa.046250Cp4.1LG19g07180Cucurbita pepo (Zucchini)cgycpeB0039
Cucsa.046250Cp4.1LG20g04440Cucurbita pepo (Zucchini)cgycpeB0040
Cucsa.046250MELO3C007280.2Melon (DHL92) v3.6.1cgymedB025
Cucsa.046250MELO3C002609.2Melon (DHL92) v3.6.1cgymedB019
Cucsa.046250CsaV3_6G050160Cucumber (Chinese Long) v3cgycucB028
Cucsa.046250CsaV3_1G012360Cucumber (Chinese Long) v3cgycucB018
Cucsa.046250Cla97C03G059500Watermelon (97103) v2cgywmbB024
Cucsa.046250Cla97C01G022910Watermelon (97103) v2cgywmbB017
Cucsa.046250Bhi03G000312Wax gourdcgywgoB026
Cucsa.046250Carg07780Silver-seed gourdcarcgyB0714
Cucsa.046250Carg15295Silver-seed gourdcarcgyB0623
Cucsa.046250Carg14623Silver-seed gourdcarcgyB0180
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cucsa.046250Cucsa.126740Cucumber (Gy14) v1cgycgyB004