Cucsa.032530 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.032530
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProtein DETOXIFICATION
Locationscaffold00429 : 1835743 .. 1838829 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGAAGAATCAAAGCTTCAACTTTTACTTTCCCTTCCATTGATTCTTACAAATGTATTCTACTATATGATAACTTTGGTCTCTGTTATGTTTGCTGATCATCTTGGTGAACTTGAGCTTGCTGCATGCTGCTACTCTTGCTAATAAATACTTTGGCTAGTATTACTGCCTTTGCTTTCGTGGTATCTCACTTTCTCTCTTTCTTTTTGTGATTTCATGATTTTGGGAAATGAGTCTTTAACTATCTCATTTATGCTGTTAAGAGATGAAAATGGAAGAAATTGGAAATGGGTTGTGTTTACAATGATAAGAAGCTCACTCTTTTTCTTCTTAAGGTCATTATTGTTCCCACCAAAATTGCAGACAAAGGTTAAGAGATCATAGGTTGAATTCAAACTCAAGGATTAATAGGTATTGATCGCTGCCTACTTAGAATTTAATATCTCATGGTTTTATTTACACTCATATATTTTAGAGTTCAGGCAACTTGTGTTGTGAGATTAGTTTAAGGGTAAATAAGCTGACTTTGATAGTCATGAATATCAATAAAAAAAaTTCAGATAAAAATCGTTTTGTGTTAGCCTATGGAACAAAATCTTTATCTCAGAGAAGATGAGTGAATTTCTAATTCTTCTTCATCAATAGTTAGGGAGAATAGTTTGATGTCCAAGATTTTtGTTTTAGGTCCACATAGGCCTCAGTTACTGCTtTTTTTTtAAAAAAAaAaTGAAAGAATGTAGGTTCTTTTGAAACAAAGGAGAAAGTAAAGTTCAGGGTACTTTTATAATTAAAGTTATAATTAATTATGATTCTGAAAAAATTCTTGTGAACTTGATGGGTAACTTCTCATTTCTCAACATGTTGAAGGGAAGCAAGCAAGGATTGGATGACTGAAAAGATTGTCAACATCTATATAGAATCACAAAATTCTCCTTTTATTGGCATAGTTGAAACTTTCTAAAGTTAAGGATCAACACAACTATGAAAGTTGAAGCATCAAATTTGCTATTAATAGCATATCAAGCCATTAAAAGATGCCTCTACTTGGTGGTATGATCTGTTGTGTGGTAATCTCTAATATTTACAATATTTCAGCCCATAGCATATAAGAAATGGAGTATTCAACTATTATGAGGATGTCAAAATTTGTTGCATTTTCCTATCCAAAGCTCATTAACAACACTAAAACTCAACTTTACCTCGTTAAAATTTGTTCTAAATTCCAAAGAGAATTTGCCTATGATTATGATAACTCGGCTTTATCTGTTTTGGTTTTAGTAGTGTACCTCTTTAGGCAGTTAACTTGTTTCTTGTTCTTTAGACTGGACTGAGCGGAGCGCTTGAAACACTTTGTGGCCAGGCATTTGGTAGGAAGTTTTATGGGAAGATGGGGTTGTATCTTCAAGGCTCTTGCATCTTAACATTCTTTTGTTCCATCATTCTATCGGTTTTATGGATTTAAACAGAACAAATTCTAGTGTTGCTCCATCAAGAACCAGAAATTTCAAGGATATCTGCAATGTATATGAAGTTTCTCATCCCAAAATTATTTGCATATGGCTTACTCCAAAACATCATGAGATTCCTCCAAACACAGTTTGTTGTCATGCCTTTGGTTCTCTTTTCTAGTGTCCCAATGCTCATCCACATACCTATTGCATACGGTCTTGTTCACTAGACACATCTCGGTTTCAATGGAGCTTCATTAGCTGCTTTAATCTCATTATGGGTGGCATGCCTGATTTTGGCCATATGTGTACTTAAAGCAAACAAGTTTGAGAAATCTTAGAATGGCTTCTCATTAGAAGCTTTTAGCTATAGTCTTTCAAGCTTAAAGCTAGCTATTCATCTGCAGCAATGTTATGGTAAGCATCCTCCACTTTTTGCCTTGAATGAAGCTTATGGAATAACATGTCGATTGGTCAACCAATTGGTAACTCATGGGTCTTTCTTGCTTTCTGTTATTATAATCGGTTAAAGTTTGGAGTATTGGACGGTCGAGGTTATGGTTTTCTTAGTTGGACTGATGCCAAACTCGAAAACAAGTACTTCACTGGTAGCAATGTGGTAAGTAAATTCTATTTGTCATGTTGTTCTCAGTCCTTCCCATTTTTCTTATTTAATTTTCCTTTTCTTGATCCTTTAGTGACAGTACACAAACCATTGCCTACATGATCACTTGTGGACTCAGTGCTACAGCAAGGTTTGTTATTTCTACATAGATTGAATTGGGTTTTAATTTTATCTCAATTGAAGTATTGATATGATTAAGTGATTTTTAATCCATCAGCTTAAACTTCTAGGCATTTCCAACTAATTAACTATAATAATACTATTCCAAAATCCTTTTTATTACAATGTTCATTATTTTCTGCCCATTCTCGTTTTCTTTTATGTTATAGAATTTATTATTTTCTAGATAGACTAAAATAGTTTGAATCACAAACACAAACAATTATAACCCACAACTTATGGTAACTACTAGCTATAATAACCCACTTAACGTTCGAACATTCCCTAGTTTATTGAACTCAAATCTTAATATAATTTCCTAATTTAATCTTCACATGTACCTTATTTTATTTTGTTCACCATCTCCTTCTGCAACACTAAATATATTGAAAGTTTTCCTTCTCAAACAAATGATACTTTCTGACAGCACAAGGGTTTCAAATGAATTGGGAGCAGGCAATTTTGACAAAGCCAAAACTGCCATGTTTGCAACTCTCAAGCTTTCTGTTCCTTCCTCTGCTGGTTGTTTTAGCACTTCATTTGGTCATAACACCTGGTCTAGCTTCTTCATCAACAATATTACAATAATGGATGAGTTTTCTTCCATGGTGCCATTTCTCGCTATCTCCATAACGCTCAACTCAGTGCAGGGCGCATATCAGGTTCGGTGCTAAAGAAAACATCCTTCCCATTCATCATTGCCATCGTTCTTATAATTACCATTCAAGTGCAAGGGTAGCTAGAGGCTATGGGTGGCAGCACTTGGTTGTATACATAAACTTGTCCATGTTCTACTTTATCGGTGTCACAATATCGATCCTTCTTGGGTTTAAGCTTAGATTATATGCTAAG

mRNA sequence

ATGGTGGAAGAATCAAAGCTTCAACTTTTACTTTCCCTTCCATTGATTCTTACAAATGTATTCTACTATATGATAACTTTGGTCTCTGTTATGTTTGCTGATCATCTTGAACAAATTCTAGTGTTGCTCCATCAAGAACCAGAAATTTCAAGGATATCTGCAATGTATATGAAGTTTCTCATCCCAAAATTATTTGCATATGGCTTACTCCAAAACATCATGAGATTCCTCCAAACACAGTTTGTTGTCATGCCTTTGGTTCTCTTTTCTAGTGTCCCAATGCTCATCCACATACCTATTGCATACGGTCTTCAATGTTATGGTAAGCATCCTCCACTTTTTGCCTTGAATGAAGCTTATGGAATAACATGTCGATTGGTCAACCAATTGGTAACTCATGGTTTGGAGTATTGGACGGTCGAGGTTATGGTTTTCTTAGTTGGACTGATGCCAAACTCGAAAACAAGTACTTCACTGGTAGCAATGTGTGACAGTACACAAACCATTGCCTACATGATCACTTGTGGACTCAGTGCTACAGCAAGCACAAGGGTTTCAAATGAATTGGGAGCAGGCAATTTTGACAAAGCCAAAACTGCCATGTTTGCAACTCTCAAGCTTTCTGTTCCTTCCTCTGCTGGTTGTTTTAGCACTTCATTTGGTCATAACACCTGGTCTAGCTTCTTCATCAACAATATTACAATAATGGATGAGTTTTCTTCCATGGTGCCATTTCTCGCTATCTCCATAACGCTCAACTCAGTGCAGGGCGCATATCAGGTTCGGGTAGCTAGAGGCTATGGGTGGCAGCACTTGGTTGTATACATAAACTTGTCCATGTTCTACTTTATCGGTGTCACAATATCGATCCTTCTTGGGTTTAAGCTTAGATTATATGCTAAG

Coding sequence (CDS)

ATGGTGGAAGAATCAAAGCTTCAACTTTTACTTTCCCTTCCATTGATTCTTACAAATGTATTCTACTATATGATAACTTTGGTCTCTGTTATGTTTGCTGATCATCTTGAACAAATTCTAGTGTTGCTCCATCAAGAACCAGAAATTTCAAGGATATCTGCAATGTATATGAAGTTTCTCATCCCAAAATTATTTGCATATGGCTTACTCCAAAACATCATGAGATTCCTCCAAACACAGTTTGTTGTCATGCCTTTGGTTCTCTTTTCTAGTGTCCCAATGCTCATCCACATACCTATTGCATACGGTCTTCAATGTTATGGTAAGCATCCTCCACTTTTTGCCTTGAATGAAGCTTATGGAATAACATGTCGATTGGTCAACCAATTGGTAACTCATGGTTTGGAGTATTGGACGGTCGAGGTTATGGTTTTCTTAGTTGGACTGATGCCAAACTCGAAAACAAGTACTTCACTGGTAGCAATGTGTGACAGTACACAAACCATTGCCTACATGATCACTTGTGGACTCAGTGCTACAGCAAGCACAAGGGTTTCAAATGAATTGGGAGCAGGCAATTTTGACAAAGCCAAAACTGCCATGTTTGCAACTCTCAAGCTTTCTGTTCCTTCCTCTGCTGGTTGTTTTAGCACTTCATTTGGTCATAACACCTGGTCTAGCTTCTTCATCAACAATATTACAATAATGGATGAGTTTTCTTCCATGGTGCCATTTCTCGCTATCTCCATAACGCTCAACTCAGTGCAGGGCGCATATCAGGTTCGGGTAGCTAGAGGCTATGGGTGGCAGCACTTGGTTGTATACATAAACTTGTCCATGTTCTACTTTATCGGTGTCACAATATCGATCCTTCTTGGGTTTAAGCTTAGATTATATGCTAAG

Protein sequence

MVEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQFVVMPLVLFSSVPMLIHIPIAYGLQCYGKHPPLFALNEAYGITCRLVNQLVTHGLEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVPSSAGCFSTSFGHNTWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYFIGVTISILLGFKLRLYAK
Homology
[Close]
BLAST of Cucsa.032530 vs. Swiss-Prot
Match: DTX18_ARATH (Protein DETOXIFICATION 18 OS=Arabidopsis thaliana GN=DTX18 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-58
Identity = 139/332 (41.87%), Postives = 190/332 (57.23%), Query Frame = 1 

Query: 7   LQLLLSLPLILTNVFYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFA 66
           L + L    I++ VF  +IT++        E + +LL Q+P IS+ +A+YMK+L P L A
Sbjct: 109 LGIHLQSSCIVSLVFTILITILWFF----TESVFLLLRQDPSISKQAALYMKYLAPGLLA 168

Query: 67  YGLLQNIMRFLQTQFVVMPLVLFSSVPMLIHIPIAYGLQ--------------------- 126
           YG LQNI+RF QTQ +V PLVLFS +P++I+I   Y L                      
Sbjct: 169 YGFLQNILRFCQTQCIVTPLVLFSFLPLVINIGTTYALVHLAGLGFIGAPIATSISLWIA 228

Query: 127 ---------CYGKHPPL---FALNEAYGITCRLVNQLVTHG---LEYWTVEVMVFLVGLM 186
                    C  K       F++   + +   L   + +     LEYW  E++VFL GLM
Sbjct: 229 FVSLGFYVICSDKFKETWTGFSMESFHHVVLNLTLSIPSAAMVCLEYWAFEILVFLAGLM 288

Query: 187 PNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVP 246
            N + +TSLVA+C +T++I+YM+TCGLSA  STRVSNELGAGN   AK A   ++KLS+ 
Sbjct: 289 RNPEITTSLVAICVNTESISYMLTCGLSAATSTRVSNELGAGNVKGAKKATSVSVKLSLV 348

Query: 247 SSAG-CFSTSFGHNTWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGW 302
            + G   +   GH+ W   F N+  I + F+S+  FLA SITL+S+QG     VARG GW
Sbjct: 349 LALGVVIAILVGHDAWVGLFSNSHVIKEGFASLRFFLAASITLDSIQGVLS-GVARGCGW 408


HSP 2 Score: 50.1 bits (118), Expect = 5.0e-05
Identity = 23/38 (60.53%), Postives = 29/38 (76.32%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          VEE+K Q++ SLP+I TN+FYY I L SVMFA  L Q+
Sbjct: 29 VEEAKTQIIYSLPMIFTNLFYYCIPLTSVMFASQLGQL 66

[Close]
BLAST of Cucsa.032530 vs. Swiss-Prot
Match: DTX19_ARATH (Protein DETOXIFICATION 19 OS=Arabidopsis thaliana GN=DTX19 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.7e-40
Identity = 89/168 (52.98%), Postives = 115/168 (68.45%), Query Frame = 1 

Query: 135 LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNF 194
           LEYW  E++VFL G+MPN + +TSLVA+C +T+ I+YM+T GLSA ASTRVSNELGAGN 
Sbjct: 277 LEYWAFEILVFLAGVMPNPEINTSLVAICVNTEAISYMLTYGLSAAASTRVSNELGAGNV 336

Query: 195 DKAKTAMFATLKLSVPSSAGCFSTSF-GHNTWSSFFINNITIMDEFSSMVPFLAISITLN 254
             AK A   ++KLS+  + G       GH+ W   F ++  I +EF+S+  FLA SITL+
Sbjct: 337 KGAKKATSVSVKLSLVLALGVVIVLLVGHDGWVGLFSDSYVIKEEFASLRFFLAASITLD 396

Query: 255 SVQGAYQVRVARGYGWQHLVVYINLSMFYFIGVTISILLGFKLRLYAK 302
           S+QG     VARG GWQ LV  INL+ FY IG+ I+   GFKL+ YAK
Sbjct: 397 SIQGVLS-GVARGCGWQRLVTVINLATFYLIGMPIAAFCGFKLKFYAK 443


HSP 2 Score: 80.9 bits (198), Expect = 2.7e-14
Identity = 44/89 (49.44%), Postives = 60/89 (67.42%), Query Frame = 1 

Query: 16  ILTNVFYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMR 75
           I++ VF  +IT+    F    E I  LL Q+P IS+ +A+YMK+  P L AYG LQNI+R
Sbjct: 126 IVSLVFSILITI----FWFFTESIFGLLRQDPSISKQAALYMKYQAPGLLAYGFLQNILR 185

Query: 76  FLQTQFVVMPLVLFSSVPMLIHIPIAYGL 105
           F QTQ ++ PLV+FS VP++I+I  AY L
Sbjct: 186 FCQTQSIIAPLVIFSFVPLVINIATAYVL 210


HSP 3 Score: 55.1 bits (131), Expect = 1.6e-06
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          VEE+K Q++ SLP+ILTNVFYY I + SVMFA HL Q+
Sbjct: 37 VEEAKAQMIYSLPMILTNVFYYCIPITSVMFASHLGQL 74

[Close]
BLAST of Cucsa.032530 vs. Swiss-Prot
Match: DTX16_ARATH (Protein DETOXIFICATION 16 OS=Arabidopsis thaliana GN=DTX16 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.8e-34
Identity = 104/312 (33.33%), Postives = 151/312 (48.40%), Query Frame = 1 

Query: 23  YMITLVSVMFA---DHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQT 82
           +++TL S+  +    + E +LV   Q   I+ ++  Y KF+IP +FAYGLLQ   RFLQ 
Sbjct: 113 FVLTLASIPLSIIWANTEHLLVFFGQNKSIATLAGSYAKFMIPSIFAYGLLQCFNRFLQA 172

Query: 83  QFVVMPLVLFSSVPMLIHI---------------------PIAYGLQ-----CYGKHPPL 142
           Q  V P+V  S V   +H+                      I+Y L      CY K  P 
Sbjct: 173 QNNVFPVVFCSGVTTSLHVLLCWVLVFKSGLGFQGAALANSISYWLNVVLLFCYVKFSPS 232

Query: 143 F----------ALNEAYGITCRLVNQLVTHGLEYWTVEVMVFLVGLMPNSKTSTSLVAMC 202
                      AL +        V   +   LE W+ E++V L GL+PN    TS++++C
Sbjct: 233 CSLTWTGFSKEALRDILPFLRLAVPSALMVCLEMWSFELLVLLSGLLPNPVLETSVLSIC 292

Query: 203 DSTQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVPSSAGCFST-SFGH 262
            +T    +MI  GLS  ASTR+SNELGAGN   AK A+   + ++V  S    S      
Sbjct: 293 LNTSGTMWMIPFGLSGAASTRISNELGAGNPKVAKLAVRVVICIAVAESIVIGSVLILIR 352

Query: 263 NTWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFY 295
           N W   + + + ++   +SM+P LA+   L+S+Q      VARG GWQ +   INL  +Y
Sbjct: 353 NIWGLAYSSELEVVSYVASMMPILALGNFLDSLQCVLS-GVARGCGWQKIGAIINLGSYY 412

[Close]
BLAST of Cucsa.032530 vs. Swiss-Prot
Match: DTX17_ARATH (Protein DETOXIFICATION 17 OS=Arabidopsis thaliana GN=DTX17 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 8.8e-34
Identity = 105/327 (32.11%), Postives = 157/327 (48.01%), Query Frame = 1 

Query: 9   LLLSLPLILTNVFYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYG 68
           L+LS+PL             S+++A+  EQILVL+HQ+  I+ ++  Y K++IP LFAYG
Sbjct: 114 LILSVPL-------------SIIWAN-TEQILVLVHQDKSIASVAGSYAKYMIPSLFAYG 173

Query: 69  LLQNIMRFLQTQFVVMPLVLFSSVPMLIH---------------------IPIAYG---- 128
           LLQ I RFLQ Q  V P+ + S +   +H                     I ++Y     
Sbjct: 174 LLQCINRFLQAQNNVFPVFVCSGITTCLHLLLCWLFVLKTGLGYRGAALAISVSYWFNVI 233

Query: 129 -LQCYGKHPPLF----------ALNEAYGITCRLVNQLVTHGLEYWTVEVMVFLVGLMPN 188
            L CY K  P            A  E Y  +       V   LE W+ E++V   GL+PN
Sbjct: 234 LLSCYVKFSPSCSHSWTGFSKEAFQELYDFSKIAFPSAVMVCLELWSFELLVLASGLLPN 293

Query: 189 SKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVPSS 248
               TS++++C +T    + I+ GL   AS RVSNELGAGN   AK A++  + ++V   
Sbjct: 294 PVLETSVLSICLNTSLTIWQISVGLGGAASIRVSNELGAGNPQVAKLAVYVIVGIAVAEG 353

Query: 249 AGCFSTSFG-HNTWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQH 299
               +            F ++  I+   +SM+P +A    L+ +Q      VARG GWQ 
Sbjct: 354 IVVVTVLLSIRKILGHAFSSDPKIIAYAASMIPIVACGNFLDGLQCVLS-GVARGCGWQK 413

[Close]
BLAST of Cucsa.032530 vs. Swiss-Prot
Match: DTX1_ARATH (Protein DETOXIFICATION 1 OS=Arabidopsis thaliana GN=DTX1 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.0e-33
Identity = 95/312 (30.45%), Postives = 156/312 (50.00%), Query Frame = 1 

Query: 27  LVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQFVVMPL 86
           L+S+++  ++E+IL+ L Q+PEISRI+  Y  +LIP LF   ++  + RFL TQ +V+PL
Sbjct: 122 LISILWL-YIEKILISLGQDPEISRIAGSYAFWLIPALFGQAIVIPLSRFLLTQGLVIPL 181

Query: 87  VLFSSVPMLIHIPIA------YGLQCYGK----------HPPLFALNEAYGITCRL---- 146
           +  +   +L H+ +       +GL C G           +  + +    +  +C      
Sbjct: 182 LFTAVTTLLFHVLVCWTLVFLFGLGCNGPAMATSVSFWFYAVILSCYVRFSSSCEKTRGF 241

Query: 147 --------VNQLVTHG--------LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIA 206
                   + Q   +G        LE+W  E+++   GL+PN K  TS++++C + +T+ 
Sbjct: 242 VSRDFVSSIKQFFQYGIPSAAMICLEWWLFEILILCSGLLPNPKLETSVLSICLTIETLH 301

Query: 207 YMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVPSSAGCFSTSFG-HNTWSSFF 266
           Y+I+ G++A  STRVSN LGAGN   A+ ++ A L L +  SA      F   N     F
Sbjct: 302 YVISAGVAAAVSTRVSNNLGAGNPQVARVSVLAGLCLWIVESAFFSILLFTCRNIIGYAF 361

Query: 267 INNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYFIGVTIS 302
            N+  ++D  + + P L +S  L+         VARG GWQH+  + N   +Y +G  + 
Sbjct: 362 SNSKEVLDYVADLTPLLCLSFILDGFTAVLN-GVARGSGWQHIGAWNNTVSYYLVGAPVG 421

[Close]
BLAST of Cucsa.032530 vs. TrEMBL
Match: A0A0A0L783_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G233980 PE=4 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-69
Identity = 146/168 (86.90%), Postives = 149/168 (88.69%), Query Frame = 1 

Query: 135 LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNF 194
           LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNF
Sbjct: 4   LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNF 63

Query: 195 DKAKTAMFATLKLSV-PSSAGCFSTSFGHNTWSSFFINNITIMDEFSSMVPFLAISITLN 254
           DKAKTAMFATLKLSV        + +FGHNTWSSFFINNITIMDEFSSMVPFLAISITL+
Sbjct: 64  DKAKTAMFATLKLSVLLPLLVVLALAFGHNTWSSFFINNITIMDEFSSMVPFLAISITLD 123

Query: 255 SVQGAYQVRVARGYGWQHLVVYINLSMFYFIGVTISILLGFKLRLYAK 302
           SVQGAY          QHLVVYINLSMFYFIGVTISILLGFKLRLYAK
Sbjct: 124 SVQGAY----------QHLVVYINLSMFYFIGVTISILLGFKLRLYAK 161

[Close]
BLAST of Cucsa.032530 vs. TrEMBL
Match: U7DZ93_POPTR (Protein DETOXIFICATION OS=Populus trichocarpa GN=POPTR_0131s00210g PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.0e-69
Identity = 159/318 (50.00%), Postives = 203/318 (63.84%), Query Frame = 1 

Query: 21  FYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQ 80
           F + IT+  + F  + E IL+LLHQ+  +S  +A+YMK+LIP LFAYG++QNI+RFLQTQ
Sbjct: 128 FIFCITISVIWF--YTEPILLLLHQDAHVSMTAALYMKYLIPGLFAYGIMQNILRFLQTQ 187

Query: 81  FVVMPLVLFSSVPMLIHIPIAYGLQCYG----KHPPLFA--------LNEAYGITCRLVN 140
            VVMP V+FS VP+ IHI IAY L  Y     K  PL A        L  A  + C    
Sbjct: 188 SVVMPPVVFSLVPLCIHIGIAYALVHYTALGFKGAPLAASISLWISVLMLAIYVICAKKF 247

Query: 141 QLVTHG------------------------LEYWTVEVMVFLVGLMPNSKTSTSLVAMCD 200
           +    G                        LEYW  E++VFL GLMP+S+ STSL+A+C 
Sbjct: 248 EHTWGGFSFESFHYILHDLKLALPSAAMVCLEYWAFEILVFLAGLMPSSEISTSLIAICV 307

Query: 201 STQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSV-PSSAGCFSTSFGHN 260
           +T+T+AYM+T GLSA ASTRVSNELG GN ++AK AM  TLKLSV  +     + +FGHN
Sbjct: 308 NTETVAYMLTYGLSAAASTRVSNELGEGNPERAKNAMAVTLKLSVLLALLVVLALAFGHN 367

Query: 261 TWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYF 302
            W+  F ++ TI  EF+SM PFLAISITL+SVQG +   VARG GWQHL VY NL+ FY 
Sbjct: 368 IWAGLFSSSPTIAKEFASMAPFLAISITLDSVQGVFS-GVARGCGWQHLAVYANLATFYC 427

[Close]
BLAST of Cucsa.032530 vs. TrEMBL
Match: U7DZ93_POPTR (Protein DETOXIFICATION OS=Populus trichocarpa GN=POPTR_0131s00210g PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 7.1e-06
Identity = 28/38 (73.68%), Postives = 34/38 (89.47%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          VEE+K Q+L SLP+ILTNVFYY+ITLVSVMFA HL ++
Sbjct: 36 VEEAKNQILFSLPMILTNVFYYLITLVSVMFAGHLGEL 73


HSP 2 Score: 270.8 bits (691), Expect = 2.0e-69
Identity = 159/318 (50.00%), Postives = 203/318 (63.84%), Query Frame = 1 

Query: 21  FYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQ 80
           F + IT+  + F  + E IL+LLHQ+  +S  +A+YMK+LIP LFAYG++QNI+RFLQTQ
Sbjct: 36  FIFCITISVIWF--YTEPILLLLHQDAHVSMTAALYMKYLIPGLFAYGIMQNILRFLQTQ 95

Query: 81  FVVMPLVLFSSVPMLIHIPIAYGLQCYG----KHPPLFA--------LNEAYGITCRLVN 140
            VVMP V+FS VP+ IHI IAY L  Y     K  PL A        L  A  + C    
Sbjct: 96  SVVMPPVVFSLVPLCIHIGIAYALVHYTALGFKGAPLAASISLWISVLMLAIYVICAKKF 155

Query: 141 QLVTHG------------------------LEYWTVEVMVFLVGLMPNSKTSTSLVAMCD 200
           +    G                        LEYW  E++VFL GLMP+S+ STSL+A+C 
Sbjct: 156 EHTWGGFSFESFHYILHDLKLALPSAAMVCLEYWAFEILVFLAGLMPSSEISTSLIAICV 215

Query: 201 STQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSV-PSSAGCFSTSFGHN 260
           +T+T+AYM+T GLSA ASTRVSNELG GN ++AK AM  TLKLSV  +     + +FGHN
Sbjct: 216 NTETVAYMLTYGLSAAASTRVSNELGEGNPERAKNAMAVTLKLSVLLALLVVLALAFGHN 275

Query: 261 TWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYF 302
            W+  F ++ TI  EF+SM PFLAISITL+SVQG +   VARG GWQHL VY NL+ FY 
Sbjct: 276 IWAGLFSSSPTIAKEFASMAPFLAISITLDSVQGVFS-GVARGCGWQHLAVYANLATFYC 335

[Close]
BLAST of Cucsa.032530 vs. TrEMBL
Match: U5FWG3_POPTR (Protein DETOXIFICATION OS=Populus trichocarpa GN=POPTR_0011s11810g PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-68
Identity = 157/318 (49.37%), Postives = 200/318 (62.89%), Query Frame = 1 

Query: 21  FYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQ 80
           F + IT+  + F  + E IL+LLHQ+  +S  +A+YMK+LIP LFAYG++QNI+RFLQTQ
Sbjct: 128 FIFCITISVIWF--YTEPILLLLHQDAHVSMTAALYMKYLIPGLFAYGIMQNILRFLQTQ 187

Query: 81  FVVMPLVLFSSVPMLIHIPIAYGLQCYG----KHPPL---------FALNEAYGITCRLV 140
            VVMP V+FS +P+ IHI IAY L  Y     K  PL         F +   Y I  +  
Sbjct: 188 SVVMPPVVFSLIPLCIHIGIAYALVHYTALGFKGAPLAASISLWISFLMLAIYVICAKKF 247

Query: 141 N-----------QLVTHG------------LEYWTVEVMVFLVGLMPNSKTSTSLVAMCD 200
                         + H             LEYW  E++VFL GLMP S+ STSL+A+C 
Sbjct: 248 EHTWAGFSFESFHYILHDLKLALPSAAMVCLEYWAFEILVFLAGLMPGSEISTSLIAICV 307

Query: 201 STQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSV-PSSAGCFSTSFGHN 260
           +T+T+AYM+T GLSA ASTRVSNELG GN D+AK AM  TLKLSV  +     + +FGHN
Sbjct: 308 NTETVAYMLTYGLSAAASTRVSNELGEGNPDRAKNAMAVTLKLSVLLALLVVLALAFGHN 367

Query: 261 TWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYF 302
            W+  F ++ TI  EF+SM PFLAISI L+SVQG     VARG GWQHL VY NL+ FY 
Sbjct: 368 IWAGLFSSSPTIAKEFASMAPFLAISIALDSVQGVLS-GVARGCGWQHLAVYANLATFYC 427

[Close]
BLAST of Cucsa.032530 vs. TrEMBL
Match: U5FWG3_POPTR (Protein DETOXIFICATION OS=Populus trichocarpa GN=POPTR_0011s11810g PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 7.1e-06
Identity = 28/38 (73.68%), Postives = 34/38 (89.47%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          VEE+K Q+L SLP+ILTNVFYY+ITLVSVMFA HL ++
Sbjct: 36 VEEAKNQILFSLPMILTNVFYYLITLVSVMFAGHLGEL 73


HSP 2 Score: 268.1 bits (684), Expect = 1.3e-68
Identity = 157/318 (49.37%), Postives = 200/318 (62.89%), Query Frame = 1 

Query: 21  FYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQ 80
           F + IT+  + F  + E IL+LLHQ+  +S  +A+YMK+LIP LFAYG++QNI+RFLQTQ
Sbjct: 128 FIFCITISVIWF--YTEPILLLLHQDAHVSMTAALYMKYLIPGLFAYGIMQNILRFLQTQ 187

Query: 81  FVVMPLVLFSSVPMLIHIPIAYGLQCYG----KHPPL---------FALNEAYGITCRLV 140
            VVMP V+FS +P+ IHI IAY L  Y     K  PL         F +   Y I  +  
Sbjct: 188 SVVMPPVVFSLIPLCIHIGIAYALVHYTALGFKGAPLAASISLWISFLMLAIYVICAKKF 247

Query: 141 N-----------QLVTHG------------LEYWTVEVMVFLVGLMPNSKTSTSLVAMCD 200
                         + H             LEYW  E++VFL GLMP S+ STSL+A+C 
Sbjct: 248 EHTWAGFSFESFHYILHDLKLALPSAAMVCLEYWAFEILVFLAGLMPGSEISTSLIAICV 307

Query: 201 STQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSV-PSSAGCFSTSFGHN 260
           +T+T+AYM+T GLSA ASTRVSNELG GN D+AK AM  TLKLSV  +     + +FGHN
Sbjct: 308 NTETVAYMLTYGLSAAASTRVSNELGEGNPDRAKNAMAVTLKLSVLLALLVVLALAFGHN 367

Query: 261 TWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYF 302
            W+  F ++ TI  EF+SM PFLAISI L+SVQG     VARG GWQHL VY NL+ FY 
Sbjct: 368 IWAGLFSSSPTIAKEFASMAPFLAISIALDSVQGVLS-GVARGCGWQHLAVYANLATFYC 427

[Close]
BLAST of Cucsa.032530 vs. TAIR10
Match: AT3G23550.1 (AT3G23550.1 MATE efflux family protein)

HSP 1 Score: 226.9 bits (577), Expect = 1.7e-59
Identity = 139/332 (41.87%), Postives = 190/332 (57.23%), Query Frame = 1 

Query: 7   LQLLLSLPLILTNVFYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFA 66
           L + L    I++ VF  +IT++        E + +LL Q+P IS+ +A+YMK+L P L A
Sbjct: 109 LGIHLQSSCIVSLVFTILITILWFF----TESVFLLLRQDPSISKQAALYMKYLAPGLLA 168

Query: 67  YGLLQNIMRFLQTQFVVMPLVLFSSVPMLIHIPIAYGLQ--------------------- 126
           YG LQNI+RF QTQ +V PLVLFS +P++I+I   Y L                      
Sbjct: 169 YGFLQNILRFCQTQCIVTPLVLFSFLPLVINIGTTYALVHLAGLGFIGAPIATSISLWIA 228

Query: 127 ---------CYGKHPPL---FALNEAYGITCRLVNQLVTHG---LEYWTVEVMVFLVGLM 186
                    C  K       F++   + +   L   + +     LEYW  E++VFL GLM
Sbjct: 229 FVSLGFYVICSDKFKETWTGFSMESFHHVVLNLTLSIPSAAMVCLEYWAFEILVFLAGLM 288

Query: 187 PNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVP 246
            N + +TSLVA+C +T++I+YM+TCGLSA  STRVSNELGAGN   AK A   ++KLS+ 
Sbjct: 289 RNPEITTSLVAICVNTESISYMLTCGLSAATSTRVSNELGAGNVKGAKKATSVSVKLSLV 348

Query: 247 SSAG-CFSTSFGHNTWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGW 302
            + G   +   GH+ W   F N+  I + F+S+  FLA SITL+S+QG     VARG GW
Sbjct: 349 LALGVVIAILVGHDAWVGLFSNSHVIKEGFASLRFFLAASITLDSIQGVLS-GVARGCGW 408


HSP 2 Score: 50.1 bits (118), Expect = 2.8e-06
Identity = 23/38 (60.53%), Postives = 29/38 (76.32%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          VEE+K Q++ SLP+I TN+FYY I L SVMFA  L Q+
Sbjct: 29 VEEAKTQIIYSLPMIFTNLFYYCIPLTSVMFASQLGQL 66

[Close]
BLAST of Cucsa.032530 vs. TAIR10
Match: AT3G23560.1 (AT3G23560.1 MATE efflux family protein)

HSP 1 Score: 166.8 bits (421), Expect = 2.1e-41
Identity = 89/168 (52.98%), Postives = 115/168 (68.45%), Query Frame = 1 

Query: 135 LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNF 194
           LEYW  E++VFL G+MPN + +TSLVA+C +T+ I+YM+T GLSA ASTRVSNELGAGN 
Sbjct: 277 LEYWAFEILVFLAGVMPNPEINTSLVAICVNTEAISYMLTYGLSAAASTRVSNELGAGNV 336

Query: 195 DKAKTAMFATLKLSVPSSAGCFSTSF-GHNTWSSFFINNITIMDEFSSMVPFLAISITLN 254
             AK A   ++KLS+  + G       GH+ W   F ++  I +EF+S+  FLA SITL+
Sbjct: 337 KGAKKATSVSVKLSLVLALGVVIVLLVGHDGWVGLFSDSYVIKEEFASLRFFLAASITLD 396

Query: 255 SVQGAYQVRVARGYGWQHLVVYINLSMFYFIGVTISILLGFKLRLYAK 302
           S+QG     VARG GWQ LV  INL+ FY IG+ I+   GFKL+ YAK
Sbjct: 397 SIQGVLS-GVARGCGWQRLVTVINLATFYLIGMPIAAFCGFKLKFYAK 443


HSP 2 Score: 80.9 bits (198), Expect = 1.5e-15
Identity = 44/89 (49.44%), Postives = 60/89 (67.42%), Query Frame = 1 

Query: 16  ILTNVFYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMR 75
           I++ VF  +IT+    F    E I  LL Q+P IS+ +A+YMK+  P L AYG LQNI+R
Sbjct: 126 IVSLVFSILITI----FWFFTESIFGLLRQDPSISKQAALYMKYQAPGLLAYGFLQNILR 185

Query: 76  FLQTQFVVMPLVLFSSVPMLIHIPIAYGL 105
           F QTQ ++ PLV+FS VP++I+I  AY L
Sbjct: 186 FCQTQSIIAPLVIFSFVPLVINIATAYVL 210


HSP 3 Score: 55.1 bits (131), Expect = 8.8e-08
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          VEE+K Q++ SLP+ILTNVFYY I + SVMFA HL Q+
Sbjct: 37 VEEAKAQMIYSLPMILTNVFYYCIPITSVMFASHLGQL 74

[Close]
BLAST of Cucsa.032530 vs. TAIR10
Match: AT5G52450.1 (AT5G52450.1 MATE efflux family protein)

HSP 1 Score: 146.0 bits (367), Expect = 3.8e-35
Identity = 104/312 (33.33%), Postives = 151/312 (48.40%), Query Frame = 1 

Query: 23  YMITLVSVMFA---DHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQT 82
           +++TL S+  +    + E +LV   Q   I+ ++  Y KF+IP +FAYGLLQ   RFLQ 
Sbjct: 113 FVLTLASIPLSIIWANTEHLLVFFGQNKSIATLAGSYAKFMIPSIFAYGLLQCFNRFLQA 172

Query: 83  QFVVMPLVLFSSVPMLIHI---------------------PIAYGLQ-----CYGKHPPL 142
           Q  V P+V  S V   +H+                      I+Y L      CY K  P 
Sbjct: 173 QNNVFPVVFCSGVTTSLHVLLCWVLVFKSGLGFQGAALANSISYWLNVVLLFCYVKFSPS 232

Query: 143 F----------ALNEAYGITCRLVNQLVTHGLEYWTVEVMVFLVGLMPNSKTSTSLVAMC 202
                      AL +        V   +   LE W+ E++V L GL+PN    TS++++C
Sbjct: 233 CSLTWTGFSKEALRDILPFLRLAVPSALMVCLEMWSFELLVLLSGLLPNPVLETSVLSIC 292

Query: 203 DSTQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVPSSAGCFST-SFGH 262
            +T    +MI  GLS  ASTR+SNELGAGN   AK A+   + ++V  S    S      
Sbjct: 293 LNTSGTMWMIPFGLSGAASTRISNELGAGNPKVAKLAVRVVICIAVAESIVIGSVLILIR 352

Query: 263 NTWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFY 295
           N W   + + + ++   +SM+P LA+   L+S+Q      VARG GWQ +   INL  +Y
Sbjct: 353 NIWGLAYSSELEVVSYVASMMPILALGNFLDSLQCVLS-GVARGCGWQKIGAIINLGSYY 412

[Close]
BLAST of Cucsa.032530 vs. TAIR10
Match: AT1G73700.1 (AT1G73700.1 MATE efflux family protein)

HSP 1 Score: 145.6 bits (366), Expect = 5.0e-35
Identity = 105/327 (32.11%), Postives = 157/327 (48.01%), Query Frame = 1 

Query: 9   LLLSLPLILTNVFYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYG 68
           L+LS+PL             S+++A+  EQILVL+HQ+  I+ ++  Y K++IP LFAYG
Sbjct: 114 LILSVPL-------------SIIWAN-TEQILVLVHQDKSIASVAGSYAKYMIPSLFAYG 173

Query: 69  LLQNIMRFLQTQFVVMPLVLFSSVPMLIH---------------------IPIAYG---- 128
           LLQ I RFLQ Q  V P+ + S +   +H                     I ++Y     
Sbjct: 174 LLQCINRFLQAQNNVFPVFVCSGITTCLHLLLCWLFVLKTGLGYRGAALAISVSYWFNVI 233

Query: 129 -LQCYGKHPPLF----------ALNEAYGITCRLVNQLVTHGLEYWTVEVMVFLVGLMPN 188
            L CY K  P            A  E Y  +       V   LE W+ E++V   GL+PN
Sbjct: 234 LLSCYVKFSPSCSHSWTGFSKEAFQELYDFSKIAFPSAVMVCLELWSFELLVLASGLLPN 293

Query: 189 SKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVPSS 248
               TS++++C +T    + I+ GL   AS RVSNELGAGN   AK A++  + ++V   
Sbjct: 294 PVLETSVLSICLNTSLTIWQISVGLGGAASIRVSNELGAGNPQVAKLAVYVIVGIAVAEG 353

Query: 249 AGCFSTSFG-HNTWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQH 299
               +            F ++  I+   +SM+P +A    L+ +Q      VARG GWQ 
Sbjct: 354 IVVVTVLLSIRKILGHAFSSDPKIIAYAASMIPIVACGNFLDGLQCVLS-GVARGCGWQK 413

[Close]
BLAST of Cucsa.032530 vs. TAIR10
Match: AT2G04040.1 (AT2G04040.1 MATE efflux family protein)

HSP 1 Score: 144.4 bits (363), Expect = 1.1e-34
Identity = 95/312 (30.45%), Postives = 156/312 (50.00%), Query Frame = 1 

Query: 27  LVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQFVVMPL 86
           L+S+++  ++E+IL+ L Q+PEISRI+  Y  +LIP LF   ++  + RFL TQ +V+PL
Sbjct: 122 LISILWL-YIEKILISLGQDPEISRIAGSYAFWLIPALFGQAIVIPLSRFLLTQGLVIPL 181

Query: 87  VLFSSVPMLIHIPIA------YGLQCYGK----------HPPLFALNEAYGITCRL---- 146
           +  +   +L H+ +       +GL C G           +  + +    +  +C      
Sbjct: 182 LFTAVTTLLFHVLVCWTLVFLFGLGCNGPAMATSVSFWFYAVILSCYVRFSSSCEKTRGF 241

Query: 147 --------VNQLVTHG--------LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIA 206
                   + Q   +G        LE+W  E+++   GL+PN K  TS++++C + +T+ 
Sbjct: 242 VSRDFVSSIKQFFQYGIPSAAMICLEWWLFEILILCSGLLPNPKLETSVLSICLTIETLH 301

Query: 207 YMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSVPSSAGCFSTSFG-HNTWSSFF 266
           Y+I+ G++A  STRVSN LGAGN   A+ ++ A L L +  SA      F   N     F
Sbjct: 302 YVISAGVAAAVSTRVSNNLGAGNPQVARVSVLAGLCLWIVESAFFSILLFTCRNIIGYAF 361

Query: 267 INNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYFIGVTIS 302
            N+  ++D  + + P L +S  L+         VARG GWQH+  + N   +Y +G  + 
Sbjct: 362 SNSKEVLDYVADLTPLLCLSFILDGFTAVLN-GVARGSGWQHIGAWNNTVSYYLVGAPVG 421

[Close]
BLAST of Cucsa.032530 vs. NCBI nr
Match: gi|659112022|ref|XP_008456028.1| (PREDICTED: MATE efflux family protein LAL5-like [Cucumis melo])

HSP 1 Score: 345.9 bits (886), Expect = 7.1e-92
Identity = 201/317 (63.41%), Postives = 227/317 (71.61%), Query Frame = 1 

Query: 22  YYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQF 81
           ++   ++SV++  + EQILVLLHQEPEISRISAMYMKFLIP LFAYGLLQNIMRFLQTQ 
Sbjct: 120 FFCSIILSVLWI-YTEQILVLLHQEPEISRISAMYMKFLIPGLFAYGLLQNIMRFLQTQS 179

Query: 82  VVMPLVLFSSVPMLIHIPIAYGL-QC--YGKHPPLFALNEAYGITCRLVNQLVTHGLEY- 141
           VVMPLV FS+VPM IHIPIAY L  C   G      A + +  + C ++   V    ++ 
Sbjct: 180 VVMPLVFFSAVPMFIHIPIAYALVHCTRLGFSGAPLAASISLWVACLILAIYVLKAKKFE 239

Query: 142 -----WTVEVM---------------------------VFLVGLMPNSKTSTSLVAMCDS 201
                +++E                             VFL GLMPN +TSTSL+AMCD+
Sbjct: 240 KSWNGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLAGLMPNPETSTSLIAMCDN 299

Query: 202 TQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSV-PSSAGCFSTSFGHNT 261
           TQTIAYMITCGLSA  STRVSNELGAGNFDKAKTAMFATLKLSV        + +FGHNT
Sbjct: 300 TQTIAYMITCGLSAAVSTRVSNELGAGNFDKAKTAMFATLKLSVLLPLLVVLALAFGHNT 359

Query: 262 WSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYFI 302
           WS+FFIN+ TI DEFSSMVPFLAISITL+SVQGA    VARGYGWQHL VYINLSMFYFI
Sbjct: 360 WSNFFINSNTIRDEFSSMVPFLAISITLDSVQGAIS-GVARGYGWQHLAVYINLSMFYFI 419

[Close]
BLAST of Cucsa.032530 vs. NCBI nr
Match: gi|659112022|ref|XP_008456028.1| (PREDICTED: MATE efflux family protein LAL5-like [Cucumis melo])

HSP 1 Score: 65.1 bits (157), Expect = 2.4e-07
Identity = 32/38 (84.21%), Postives = 35/38 (92.11%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          +EESKLQLL SLPLILTNVFYYMITLVSVMFA HL ++
Sbjct: 28 MEESKLQLLFSLPLILTNVFYYMITLVSVMFAGHLGEL 65


HSP 2 Score: 271.6 bits (693), Expect = 1.7e-69
Identity = 159/334 (47.60%), Postives = 207/334 (61.98%), Query Frame = 1 

Query: 5   SKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKL 64
           +K   +L + L  +++  ++ +++      + E IL+LL Q+P+IS  +A+Y+K+LIP L
Sbjct: 110 AKSYRMLGIYLQASSIISFLFSIIISAVWFYTEPILILLQQDPQISMSAAVYIKYLIPGL 169

Query: 65  FAYGLLQNIMRFLQTQFVVMPLVLFSSVPMLIHIPIAYGLQCYG----KHPPLFA----- 124
           FAYG L NI+RFLQTQ VVMPLV  S++P++ HI IAYGL  +     K  PL A     
Sbjct: 170 FAYGFLNNILRFLQTQSVVMPLVFLSAIPLVFHIGIAYGLVHWTALGFKGAPLAASISLC 229

Query: 125 ---LNEAYGITCRLVNQLVTHG------------------------LEYWTVEVMVFLVG 184
              L  A  + C    +    G                        LEYW  E++V L G
Sbjct: 230 ISFLMLATYVVCAKKFEHTWEGFSLESFHYVLTDLKLALPSAAMVCLEYWAFEILVLLAG 289

Query: 185 LMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLS 244
           LMPNS+ +TSL+AMC +T+TIAYM+T GLSA ASTRVSNELG GN D+AK AM  TLKLS
Sbjct: 290 LMPNSEQTTSLIAMCVNTETIAYMLTYGLSAAASTRVSNELGEGNPDRAKNAMAVTLKLS 349

Query: 245 VPSSA-GCFSTSFGHNTWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGY 302
           V        + + GHN W+ FF ++ TI+ +F+ M PFLAISI L+SVQG     VARG 
Sbjct: 350 VLLGVLVVLALALGHNIWAGFFSDSTTIIKQFAYMTPFLAISIMLDSVQGVLS-GVARGC 409

[Close]
BLAST of Cucsa.032530 vs. NCBI nr
Match: gi|470120896|ref|XP_004296523.1| (PREDICTED: MATE efflux family protein LAL5-like isoform X2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-05
Identity = 28/38 (73.68%), Postives = 34/38 (89.47%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          VEE+K Q+L SLP+ILTNVFYY+ITLVSVMFA HL ++
Sbjct: 36 VEEAKNQVLFSLPMILTNVFYYLITLVSVMFAGHLGEL 73


HSP 2 Score: 271.6 bits (693), Expect = 1.7e-69
Identity = 146/168 (86.90%), Postives = 149/168 (88.69%), Query Frame = 1 

Query: 135 LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNF 194
           LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNF
Sbjct: 4   LEYWTVEVMVFLVGLMPNSKTSTSLVAMCDSTQTIAYMITCGLSATASTRVSNELGAGNF 63

Query: 195 DKAKTAMFATLKLSV-PSSAGCFSTSFGHNTWSSFFINNITIMDEFSSMVPFLAISITLN 254
           DKAKTAMFATLKLSV        + +FGHNTWSSFFINNITIMDEFSSMVPFLAISITL+
Sbjct: 64  DKAKTAMFATLKLSVLLPLLVVLALAFGHNTWSSFFINNITIMDEFSSMVPFLAISITLD 123

Query: 255 SVQGAYQVRVARGYGWQHLVVYINLSMFYFIGVTISILLGFKLRLYAK 302
           SVQGAY          QHLVVYINLSMFYFIGVTISILLGFKLRLYAK
Sbjct: 124 SVQGAY----------QHLVVYINLSMFYFIGVTISILLGFKLRLYAK 161

[Close]
BLAST of Cucsa.032530 vs. NCBI nr
Match: gi|743853396|ref|XP_011029503.1| (PREDICTED: MATE efflux family protein ALF5-like [Populus euphratica])

HSP 1 Score: 271.2 bits (692), Expect = 2.2e-69
Identity = 160/318 (50.31%), Postives = 201/318 (63.21%), Query Frame = 1 

Query: 21  FYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQ 80
           F + IT+  + F  + E IL+LLHQ+  +S  +A+YMK+LIP LFAYG++QNI+RFLQTQ
Sbjct: 128 FIFCITISVIWF--YTEPILLLLHQDAHVSMTAALYMKYLIPGLFAYGIMQNILRFLQTQ 187

Query: 81  FVVMPLVLFSSVPMLIHIPIAYGLQCYG----KHPPLFA--------LNEAYGITCRLVN 140
            VVMP V+FS VP+ IH  IAY L  Y     K  PL A        L  A  + C    
Sbjct: 188 SVVMPPVIFSLVPLCIHFGIAYALVHYTALGFKGAPLAASISLWISVLMLAVYVICAKKF 247

Query: 141 QLVTHG------------------------LEYWTVEVMVFLVGLMPNSKTSTSLVAMCD 200
           +    G                        LEYW  E++VFL GLMP+S+ STSL+A+C 
Sbjct: 248 EHTWGGFSFESFHYILHDLKLALPSAAMVCLEYWAFEILVFLAGLMPSSEISTSLIAICV 307

Query: 201 STQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSV-PSSAGCFSTSFGHN 260
           +T+T+AYM+T GLSA ASTRVSNELG GN D+AK AM  TLKLSV  +     + +FGHN
Sbjct: 308 NTETVAYMLTYGLSAAASTRVSNELGEGNPDRAKNAMAVTLKLSVLLALLVVLALAFGHN 367

Query: 261 TWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYF 302
            W+ FF ++ TI  EF+SM PFLAISITL+SVQG     VARG GWQHL VY NL+ FY 
Sbjct: 368 IWAGFFSSSPTIAKEFASMAPFLAISITLDSVQGVLS-GVARGCGWQHLAVYANLATFYC 427

[Close]
BLAST of Cucsa.032530 vs. NCBI nr
Match: gi|743853396|ref|XP_011029503.1| (PREDICTED: MATE efflux family protein ALF5-like [Populus euphratica])

HSP 1 Score: 59.7 bits (143), Expect = 1.0e-05
Identity = 28/38 (73.68%), Postives = 34/38 (89.47%), Query Frame = 1 

Query: 2  VEESKLQLLLSLPLILTNVFYYMITLVSVMFADHLEQI 40
          VEE+K Q+L SLP+ILTNVFYY+ITLVSVMFA HL ++
Sbjct: 36 VEEAKNQILFSLPMILTNVFYYLITLVSVMFAGHLGEL 73


HSP 2 Score: 270.8 bits (691), Expect = 2.9e-69
Identity = 159/318 (50.00%), Postives = 203/318 (63.84%), Query Frame = 1 

Query: 21  FYYMITLVSVMFADHLEQILVLLHQEPEISRISAMYMKFLIPKLFAYGLLQNIMRFLQTQ 80
           F + IT+  + F  + E IL+LLHQ+  +S  +A+YMK+LIP LFAYG++QNI+RFLQTQ
Sbjct: 36  FIFCITISVIWF--YTEPILLLLHQDAHVSMTAALYMKYLIPGLFAYGIMQNILRFLQTQ 95

Query: 81  FVVMPLVLFSSVPMLIHIPIAYGLQCYG----KHPPLFA--------LNEAYGITCRLVN 140
            VVMP V+FS VP+ IHI IAY L  Y     K  PL A        L  A  + C    
Sbjct: 96  SVVMPPVVFSLVPLCIHIGIAYALVHYTALGFKGAPLAASISLWISVLMLAIYVICAKKF 155

Query: 141 QLVTHG------------------------LEYWTVEVMVFLVGLMPNSKTSTSLVAMCD 200
           +    G                        LEYW  E++VFL GLMP+S+ STSL+A+C 
Sbjct: 156 EHTWGGFSFESFHYILHDLKLALPSAAMVCLEYWAFEILVFLAGLMPSSEISTSLIAICV 215

Query: 201 STQTIAYMITCGLSATASTRVSNELGAGNFDKAKTAMFATLKLSV-PSSAGCFSTSFGHN 260
           +T+T+AYM+T GLSA ASTRVSNELG GN ++AK AM  TLKLSV  +     + +FGHN
Sbjct: 216 NTETVAYMLTYGLSAAASTRVSNELGEGNPERAKNAMAVTLKLSVLLALLVVLALAFGHN 275

Query: 261 TWSSFFINNITIMDEFSSMVPFLAISITLNSVQGAYQVRVARGYGWQHLVVYINLSMFYF 302
            W+  F ++ TI  EF+SM PFLAISITL+SVQG +   VARG GWQHL VY NL+ FY 
Sbjct: 276 IWAGLFSSSPTIAKEFASMAPFLAISITLDSVQGVFS-GVARGCGWQHLAVYANLATFYC 335

The following BLAST results are available for this feature:
BLAST of Cucsa.032530 vs. Swiss-Prot
Match NameE-valueIdentityDescription
DTX18_ARATH3.0e-5841.87Protein DETOXIFICATION 18 OS=Arabidopsis thaliana GN=DTX18 PE=2 SV=1[more]
DTX19_ARATH3.7e-4052.98Protein DETOXIFICATION 19 OS=Arabidopsis thaliana GN=DTX19 PE=2 SV=1[more]
DTX16_ARATH6.8e-3433.33Protein DETOXIFICATION 16 OS=Arabidopsis thaliana GN=DTX16 PE=2 SV=1[more]
DTX17_ARATH8.8e-3432.11Protein DETOXIFICATION 17 OS=Arabidopsis thaliana GN=DTX17 PE=2 SV=1[more]
DTX1_ARATH2.0e-3330.45Protein DETOXIFICATION 1 OS=Arabidopsis thaliana GN=DTX1 PE=2 SV=1[more]
BLAST of Cucsa.032530 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L783_CUCSA1.2e-6986.90Uncharacterized protein OS=Cucumis sativus GN=Csa_3G233980 PE=4 SV=1[more]
U7DZ93_POPTR2.0e-6950.00Protein DETOXIFICATION OS=Populus trichocarpa GN=POPTR_0131s00210g PE=3 SV=1[more]
U7DZ93_POPTR7.1e-0673.68Protein DETOXIFICATION OS=Populus trichocarpa GN=POPTR_0131s00210g PE=3 SV=1[more]
U5FWG3_POPTR1.3e-6849.37Protein DETOXIFICATION OS=Populus trichocarpa GN=POPTR_0011s11810g PE=3 SV=1[more]
U5FWG3_POPTR7.1e-0673.68Protein DETOXIFICATION OS=Populus trichocarpa GN=POPTR_0011s11810g PE=3 SV=1[more]
BLAST of Cucsa.032530 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G23550.11.7e-5941.87 MATE efflux family protein[more]
AT3G23560.12.1e-4152.98 MATE efflux family protein[more]
AT5G52450.13.8e-3533.33 MATE efflux family protein[more]
AT1G73700.15.0e-3532.11 MATE efflux family protein[more]
AT2G04040.11.1e-3430.45 MATE efflux family protein[more]
BLAST of Cucsa.032530 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659112022|ref|XP_008456028.1|7.1e-9263.41PREDICTED: MATE efflux family protein LAL5-like [Cucumis melo][more]
gi|659112022|ref|XP_008456028.1|2.4e-0784.21PREDICTED: MATE efflux family protein LAL5-like [Cucumis melo][more]
gi|470120896|ref|XP_004296523.1|1.3e-0573.68PREDICTED: MATE efflux family protein LAL5-like isoform X2 [Fragaria vesca subsp... [more]
gi|743853396|ref|XP_011029503.1|2.2e-6950.31PREDICTED: MATE efflux family protein ALF5-like [Populus euphratica][more]
gi|743853396|ref|XP_011029503.1|1.0e-0573.68PREDICTED: MATE efflux family protein ALF5-like [Populus euphratica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002528MATE_fam
Vocabulary: Biological Process
TermDefinition
GO:0006855drug transmembrane transport
GO:0055085transmembrane transport
Vocabulary: Molecular Function
TermDefinition
GO:0015238drug transmembrane transporter activity
GO:0015297antiporter activity
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006855 drug transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0015238 drug transmembrane transporter activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.032530.1Cucsa.032530.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 135..288
score: 1.9E-15coord: 12..102
score: 1.
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 2..301
score: 6.1E
NoneNo IPR availablePANTHERPTHR11206:SF78MATE EFFLUX FAMILY PROTEIN ALF5-RELATEDcoord: 2..301
score: 6.1E