Cucsa.025120 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.025120
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	tRNA isopentenyltransferase
Location	scaffold00345 : 9319 .. 10210 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATTGCAAAAAGAGAAGGTTGTGATCGTGATCGGTCCCACCGGAACGGGAAAATCTCGATTGTCGATTGACATCGCCACTCGATTCCCATCGGAAGTCATAAACTCCGACAAGATGCAAGTCTACCATGGCCTTGACATAGTAACCAATAAGATCACAAAGCAAGAACAATGTGGAGTGCCTCATCACTTGCTTGGAATTATCAATCCCTTCTTGGATTTCTCCGCTTCCAACTTCTCAGATATGGCCATGCTTTCCTTGGATGCTATTTCTAGCCACGACCGATTACCGATCATCGCCGGTGGCTCGAATTCTTATATCGAAGCCTTAATCGACTCAAATTTTCGATTGAGATATGAATGTTGCTTTCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCGTTTGTATCTGAACGAGTAGACAAGATGGTAGCAAATGGAATGGTTGACGAGGCTGAAAAATTCTTCGATCCCGCTAGAGATTATTCTCGAGGAATCAAAAGAGCGATTGGAGTTCCCGAGTTCGACTCATATTTTCGATACAGGTCATTGTTGGATGAGGAAGCTCAAGAAAAACTTTTGGAGGAATCCATAGCAAAAGTGAAGGAGCATACATGTAAGCTAGCATTAAGGCAATTGGGAAAAATCCAACGGCTGAGAAACGTCAAAGGATGGAACATACATAGATTGGATGCTACAGAGGCCTTTACAAAGCAAGGGAAAGAAGCAGATGAAGCATGGAACAAGCACGTCACCATACCGAGCACGACGATAATCGATCAGTTTCTTCACCAAAACGTCATCGCCACAAAGTTCCCAACGAACTTAGCATCCCTCATCGGCGTGCCCCGGAAAAAAAGTGCAGCCATGGCAGCTGCAACTCCTTGA

mRNA sequence

AAAATTGCAAAAAGAGAAGGTTGTGATCGTGATCGGTCCCACCGGAACGGGAAAATCTCGATTGTCGATTGACATCGCCACTCGATTCCCATCGGAAGTCATAAACTCCGACAAGATGCAAGTCTACCATGGCCTTGACATAGTAACCAATAAGATCACAAAGCAAGAACAATGTGGAGTGCCTCATCACTTGCTTGGAATTATCAATCCCTTCTTGGATTTCTCCGCTTCCAACTTCTCAGATATGGCCATGCTTTCCTTGGATGCTATTTCTAGCCACGACCGATTACCGATCATCGCCGGTGGCTCGAATTCTTATATCGAAGCCTTAATCGACTCAAATTTTCGATTGAGATATGAATGTTGCTTTCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCGTTTGTATCTGAACGAGTAGACAAGATGGTAGCAAATGGAATGGTTGACGAGGCTGAAAAATTCTTCGATCCCGCTAGAGATTATTCTCGAGGAATCAAAAGAGCGATTGGAGTTCCCGAGTTCGACTCATATTTTCGATACAGGTCATTGTTGGATGAGGAAGCTCAAGAAAAACTTTTGGAGGAATCCATAGCAAAAGTGAAGGAGCATACATGTAAGCTAGCATTAAGGCAATTGGGAAAAATCCAACGGCTGAGAAACGTCAAAGGATGGAACATACATAGATTGGATGCTACAGAGGCCTTTACAAAGCAAGGGAAAGAAGCAGATGAAGCATGGAACAAGCACGTCACCATACCGAGCACGACGATAATCGATCAGTTTCTTCACCAAAACGTCATCGCCACAAATGCAGCCATGGCAGCTGCAACTCCTTGA

Coding sequence (CDS)

AAAATTGCAAAAAGAGAAGGTTGTGATCGTGATCGGTCCCACCGGAACGGGAAAATCTCGATTGTCGATTGACATCGCCACTCGATTCCCATCGGAAGTCATAAACTCCGACAAGATGCAAGTCTACCATGGCCTTGACATAGTAACCAATAAGATCACAAAGCAAGAACAATGTGGAGTGCCTCATCACTTGCTTGGAATTATCAATCCCTTCTTGGATTTCTCCGCTTCCAACTTCTCAGATATGGCCATGCTTTCCTTGGATGCTATTTCTAGCCACGACCGATTACCGATCATCGCCGGTGGCTCGAATTCTTATATCGAAGCCTTAATCGACTCAAATTTTCGATTGAGATATGAATGTTGCTTTCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCGTTTGTATCTGAACGAGTAGACAAGATGGTAGCAAATGGAATGGTTGACGAGGCTGAAAAATTCTTCGATCCCGCTAGAGATTATTCTCGAGGAATCAAAAGAGCGATTGGAGTTCCCGAGTTCGACTCATATTTTCGATACAGGTCATTGTTGGATGAGGAAGCTCAAGAAAAACTTTTGGAGGAATCCATAGCAAAAGTGAAGGAGCATACATGTAAGCTAGCATTAAGGCAATTGGGAAAAATCCAACGGCTGAGAAACGTCAAAGGATGGAACATACATAGATTGGATGCTACAGAGGCCTTTACAAAGCAAGGGAAAGAAGCAGATGAAGCATGGAACAAGCACGTCACCATACCGAGCACGACGATAATCGATCAGTTTCTTCACCAAAACGTCATCGCCACAAATGCAGCCATGGCAGCTGCAACTCCTTGA

Protein sequence

KLQKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFLHQNVIATNAAMAAATP*

Homology

[Close]

BLAST of Cucsa.025120 vs. Swiss-Prot
Match: IPT5_ARATH (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana GN=IPT5 PE=1 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 5.1e-84
Identity = 159/267 (59.55%), Postives = 202/267 (75.66%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           +K+KVV V+G TGTGKSRL+ID+ATRFP+E++NSDK+QVY GLDIVTNK+T +E  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 63  HLLGII-NPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDS--NFRLRY 122
           HLLG + + + DF+A +F   A+ ++++I   DR+PIIAGGSNSYIEAL++   +FRLRY
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVNDCVDFRLRY 150

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDP-ARDYSRGIKRAIGVPEFDS 182
            CCFLWVDVS  VL SFVSERVDKMV  G+VDE  + FDP + DYS GI+RAIGVPE D 
Sbjct: 151 NCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELDE 210

Query: 183 YFRYRSL-LDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAF 242
           + R        E  E+LLE +I K+KE+TC LA RQL KIQRL     WN+HR+DATE F
Sbjct: 211 FLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEVF 270

Query: 243 TKQGKEADEAWNKHVTIPSTTIIDQFL 265
            ++G+EADEAW+  V  PS   +++FL
Sbjct: 271 LRRGEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Cucsa.025120 vs. Swiss-Prot
Match: IPT3_ARATH (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana GN=IPT3 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.5e-83
Identity = 157/286 (54.90%), Postives = 213/286 (74.48%), Query Frame = 1


Query: 4   KEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHH 63
           K+KVV+++G TGTGKSRLS+DIATRF +E+INSDK+QV+ GLDIVTNKIT +E CGVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 64  LLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALID---SNFRLRYE 123
           LLG++ P  D +A+N+  MA LS++++ +  +LPII GGSNSY+EAL+D   + FR RY+
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYD 159

Query: 124 CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPAR-DYSRGIKRAIGVPEFDSY 183
           CCFLWVDV++ VL  FVSERVDKMV +GMV+E  +FFD +  DYSRGIK+AIG PEFD +
Sbjct: 160 CCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRF 219

Query: 184 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 243
           FR    L+ E +E+LL + + ++K +T +LA RQ  KI+RLR VK W+I R+DAT  FTK
Sbjct: 220 FRNEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTK 279

Query: 244 QGK--EADEAWNKHVTIPSTTIIDQFL-----HQNVIATNAAMAAA 279
           +    +A+ AW + V  PST  + +FL      + ++  + A+AAA
Sbjct: 280 RRSKMDANVAWERLVAGPSTDTVSRFLLDIASRRPLVEASTAVAAA 325

[Close]

BLAST of Cucsa.025120 vs. Swiss-Prot
Match: IPT7_ARATH (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana GN=IPT7 PE=2 SV=2)

HSP 1 Score: 270.0 bits (689), Expect = 2.9e-71
Identity = 137/269 (50.93%), Postives = 196/269 (72.86%), Query Frame = 1


Query: 4   KEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHH 63
           KEKV+ V+G TG+GKSRL+ID+ATRF  E+INSDK+Q+Y GLD++TNK+T +E  GVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 64  LLGIINPFL-DFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALID--SNFRLR-Y 123
           LLG+ +    + +A+ +S +A  ++  +S++++LPI+AGGSNSYIEAL++  S F L  Y
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNY 152

Query: 124 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 183
           +CCF+WVDVS+ VL SFVS+RVD+M+  G+++E  + F+P  +YS GI+RAIGVPE   Y
Sbjct: 153 DCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEY 212

Query: 184 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 243
            R  SL+D   + K+L+ ++  +K++T  LA RQL KIQRL      ++HR+DATE F K
Sbjct: 213 LRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLK 272

Query: 244 QG-KEADEAWNKHVTIPSTTIIDQFLHQN 268
           +  +E DEAW   V  PS  I+D+F + N
Sbjct: 273 RNVEEQDEAWENLVARPSERIVDKFYNNN 301

[Close]

BLAST of Cucsa.025120 vs. Swiss-Prot
Match: IPT_HUMLU (Adenylate isopentenyltransferase OS=Humulus lupulus PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 5.5e-62
Identity = 132/303 (43.56%), Postives = 188/303 (62.05%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           +KEK+++++G TGTGKSRLSID+A  FP EVINSDKMQVY GLDI TNKI+  ++ GVPH
Sbjct: 28  RKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPH 87

Query: 63  HLLGIINPFL-DFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNF------ 122
           HLLG ++P   + + ++F  +A  ++  I+   +LP++ GGSNS+I AL+   F      
Sbjct: 88  HLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPG 147

Query: 123 ------------RLRYECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPA-RD 182
                        LRY+CCFLWVDVS++VL  ++++RVD M+  GM DE  +F+ P   D
Sbjct: 148 VFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDED 207

Query: 183 YSR------GIKRAIGVPEFDSYFRYRSLLDEEAQEK--------LLEESIAKVKEHTCK 242
           +        G+++AIGVPEFD YF      D E ++           EE++  +KE+TC 
Sbjct: 208 HDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCH 267

Query: 243 LALRQLGKIQRLRNVKGWNIHRLDATEAF-----TKQGKEADEAWNKHVTIPSTTIIDQF 267
           LA RQ+GKI RL+   GW++ RLDATE+F     +  G++  E W K V  PS  I+ +F
Sbjct: 268 LAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRF 327

[Close]

BLAST of Cucsa.025120 vs. Swiss-Prot
Match: IPT1_ARATH (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana GN=IPT1 PE=1 SV=2)

HSP 1 Score: 235.0 bits (598), Expect = 1.0e-60
Identity = 130/296 (43.92%), Postives = 188/296 (63.51%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           +K+KVV+++G TG GKSRLS+D+ATRFPSE+INSDK+QVY GL+I TN+IT Q++ GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 63  HLLGIINP-FLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNF------ 122
           HLLG+INP   + +A  F   A   +  I+S  ++PIIAGGSNS++ AL+   F      
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 123 ----------RLRYECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPAR---D 182
                      LRYECCF+WVDVS  VL  ++  RVD+M+ +GM +E  +F+DP +   +
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 183 YSRGIKRAIGVPEFDSYFR----YRSLLDEEAQEK-LLEESIAKVKEHTCKLALRQLGKI 242
              GI++AIGVPEFD YF+     + ++  +A  K   ++++  +K +T  LA RQ+ KI
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 243 QRLRNVKGWNIHRLDATEAF------TKQGKEADEAWNKHVTIPSTTIIDQFLHQN 268
           + L++  GW I R+DAT +F      +   K+  E W + V  PS  I+ + L QN
Sbjct: 303 EMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

[Close]

BLAST of Cucsa.025120 vs. TrEMBL
Match: A0A0A0K919_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G392940 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 4.0e-152
Identity = 279/296 (94.26%), Postives = 280/296 (94.59%), Query Frame = 1


Query: 1   KLQKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGV 60
           KLQKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGV
Sbjct: 35  KLQKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGV 94

Query: 61  PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYE 120
           PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYE
Sbjct: 95  PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYE 154

Query: 121 CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF 180
           CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF
Sbjct: 155 CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF 214

Query: 181 RYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQ 240
           RYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQ
Sbjct: 215 RYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQ 274

Query: 241 GKEADEAWNKHVTIPSTTIIDQFLHQNVIAT----------------NAAMAAATP 281
           GKEADEAWNKHVTIPSTTIIDQFLHQNVIAT                +AAMAAATP
Sbjct: 275 GKEADEAWNKHVTIPSTTIIDQFLHQNVIATKFPTNLASLIGVPRKKSAAMAAATP 330

[Close]

BLAST of Cucsa.025120 vs. TrEMBL
Match: F6KQG8_MALDO (Adenylate isopentenyltransferase OS=Malus domestica GN=IPT3b PE=2 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 1.8e-99
Identity = 186/289 (64.36%), Postives = 225/289 (77.85%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           QKEKVVIV+G TGTGKSRLSID+AT FP+E+INSDKMQVY GLDIVTNKITK+EQ GVPH
Sbjct: 19  QKEKVVIVMGATGTGKSRLSIDLATCFPAEIINSDKMQVYEGLDIVTNKITKEEQRGVPH 78

Query: 63  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSN---FRLRY 122
           HLLGI++P  DF+A +F D+  L+++++   DRLPII GGSNSYIEAL+D +   FR +Y
Sbjct: 79  HLLGILDPNEDFTARDFCDLTSLAIESVLGRDRLPIIVGGSNSYIEALVDDHDYKFRSKY 138

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 182
           ECCFLWVDVS+ V+ SFVS RVD MV NGMVDEA +FFDP  DY +GI+RAIGVPEFD Y
Sbjct: 139 ECCFLWVDVSVPVMHSFVSGRVDHMVQNGMVDEAREFFDPNADYMKGIRRAIGVPEFDKY 198

Query: 183 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 242
           FRY  LLDEE + +LL++++ K+K++TCKLA RQL KI  LRN KGWN+H LDATE F K
Sbjct: 199 FRYGPLLDEETRARLLQQAVDKIKDNTCKLACRQLEKIHGLRNNKGWNLHPLDATEVFRK 258

Query: 243 QGKEADEAWNKHVTIPSTTIIDQFL---------HQNVIATNAAMAAAT 280
           +GK+ADEAW K V+ P   I+ QFL         H   +   AA+AAAT
Sbjct: 259 RGKDADEAWEKLVSGPGAMIVGQFLYNMTAEVPPHIGSLRVPAALAAAT 307

[Close]

BLAST of Cucsa.025120 vs. TrEMBL
Match: F6KSY3_MALDO (Adenylate isopentenyltransferase OS=Malus domestica GN=IPT3a PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.3e-98
Identity = 183/289 (63.32%), Postives = 224/289 (77.51%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           QKEKVVIV+G TGTGKSRLSID+AT FP+E+INSDKMQVY GLDIVTNKITK+EQ GVPH
Sbjct: 31  QKEKVVIVMGATGTGKSRLSIDLATCFPAEIINSDKMQVYEGLDIVTNKITKEEQRGVPH 90

Query: 63  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDS---NFRLRY 122
           HLLG+++P  DF+A +F D+  +++++I   DRLPII GGSNSYIEALID     FR +Y
Sbjct: 91  HLLGMLDPHEDFTARDFCDLTSIAIESILGRDRLPIIVGGSNSYIEALIDDYDYKFRSKY 150

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 182
           +CCFLWVDVS  VL SFVS+RVD MV NGMVDEA ++FDP  DY++GI+RAIGVPEFD Y
Sbjct: 151 DCCFLWVDVSTPVLHSFVSKRVDHMVHNGMVDEAREYFDPNADYTKGIRRAIGVPEFDKY 210

Query: 183 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 242
           FRY   LDEE + +LL +++ ++K++TCKLA RQL KI RLRN+KGWN+H LDATE F K
Sbjct: 211 FRYGPFLDEETRTRLLRQAVDEIKDNTCKLACRQLEKIHRLRNIKGWNLHPLDATEVFRK 270

Query: 243 QGKEADEAWNKHVTIPSTTIIDQFL---------HQNVIATNAAMAAAT 280
           +G+EADEAW K V+  S  I+ QFL         H   +    A+AAAT
Sbjct: 271 RGEEADEAWKKLVSGTSAMIVGQFLYNMTAEVPPHLGSLRVLEALAAAT 319

[Close]

BLAST of Cucsa.025120 vs. TrEMBL
Match: F6KQG7_MALDO (Adenylate isopentenyltransferase OS=Malus domestica GN=IPT3a PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 7.4e-98
Identity = 182/289 (62.98%), Postives = 224/289 (77.51%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           QKEKVVIV+G TGTGKSRLSID+AT FP+E+INSDKMQVY GLDIVTNKITK+EQ GVPH
Sbjct: 31  QKEKVVIVMGATGTGKSRLSIDLATCFPAEIINSDKMQVYEGLDIVTNKITKEEQRGVPH 90

Query: 63  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDS---NFRLRY 122
           HLLG+++P  DF+A +F D+  +++++I   DRLPII GGSNSYIEALID     FR +Y
Sbjct: 91  HLLGMLDPHEDFTARDFCDLTSIAIESILGRDRLPIIVGGSNSYIEALIDDYDYKFRSKY 150

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 182
           +CCFLWVDVS  VL SFVS+RVD MV NGMVDEA ++FDP  DY++GI+RAIGVPEFD Y
Sbjct: 151 DCCFLWVDVSTPVLHSFVSKRVDHMVQNGMVDEAREYFDPNADYTKGIRRAIGVPEFDKY 210

Query: 183 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 242
           FRY   L+EE + +LL +++ ++K++TCKLA RQL KI RLRN+KGWN+H LDATE F K
Sbjct: 211 FRYGPFLEEETRARLLRQAVDEIKDNTCKLACRQLEKIHRLRNIKGWNLHPLDATEVFRK 270

Query: 243 QGKEADEAWNKHVTIPSTTIIDQFL---------HQNVIATNAAMAAAT 280
           +G+EADEAW K V+  S  I+ QFL         H   +    A+AAAT
Sbjct: 271 RGEEADEAWKKLVSGTSAMIVGQFLYNMTAEVPPHLGSLRVLEALAAAT 319

[Close]

BLAST of Cucsa.025120 vs. TrEMBL
Match: M5VLB1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023171mg PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.3e-97
Identity = 186/293 (63.48%), Postives = 223/293 (76.11%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           QKEKVVIV+G TGTGKSRLSID+AT   +E+INSDKMQVY GLDI TNKIT++EQ GVPH
Sbjct: 29  QKEKVVIVMGATGTGKSRLSIDLATHLAAEIINSDKMQVYKGLDIATNKITEEEQRGVPH 88

Query: 63  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDS---NFRLRY 122
           HLLGI++P  DF+A++F D   L++++I   DRLPII GGSNSYIEALID     FR +Y
Sbjct: 89  HLLGILDPNEDFTATDFCDETSLTIESILGRDRLPIIVGGSNSYIEALIDDYDYKFRSKY 148

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 182
           ECCFLWV+VS  VL SFVS+RVDKMV NGMVDE  +FF P  DYS+GI+RAIGVPEFD Y
Sbjct: 149 ECCFLWVNVSTPVLHSFVSKRVDKMVENGMVDEVREFFHPNADYSKGIRRAIGVPEFDKY 208

Query: 183 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 242
           FRY   LDEE + +LLE+++ ++K++ CKLA RQL KIQRLRNVKGWN+H LDATE F K
Sbjct: 209 FRYGPFLDEETKARLLEQAVEEIKKNNCKLASRQLEKIQRLRNVKGWNLHPLDATEVFRK 268

Query: 243 QGKEADEAWNKHVTIPSTTIIDQFLHQNVIA-------------TNAAMAAAT 280
           +GKE+DEAW K V  PS  I+ QFL+    A              +AA+AAAT
Sbjct: 269 RGKESDEAWEKFVYGPSAQIVRQFLYNYATAEVPVNLGAMRVPMESAALAAAT 321

[Close]

BLAST of Cucsa.025120 vs. TAIR10
Match: AT5G19040.1 (AT5G19040.1 isopentenyltransferase 5)

HSP 1 Score: 312.4 bits (799), Expect = 2.9e-85
Identity = 159/267 (59.55%), Postives = 202/267 (75.66%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           +K+KVV V+G TGTGKSRL+ID+ATRFP+E++NSDK+QVY GLDIVTNK+T +E  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 63  HLLGII-NPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDS--NFRLRY 122
           HLLG + + + DF+A +F   A+ ++++I   DR+PIIAGGSNSYIEAL++   +FRLRY
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVNDCVDFRLRY 150

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDP-ARDYSRGIKRAIGVPEFDS 182
            CCFLWVDVS  VL SFVSERVDKMV  G+VDE  + FDP + DYS GI+RAIGVPE D 
Sbjct: 151 NCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELDE 210

Query: 183 YFRYRSL-LDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAF 242
           + R        E  E+LLE +I K+KE+TC LA RQL KIQRL     WN+HR+DATE F
Sbjct: 211 FLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEVF 270

Query: 243 TKQGKEADEAWNKHVTIPSTTIIDQFL 265
            ++G+EADEAW+  V  PS   +++FL
Sbjct: 271 LRRGEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Cucsa.025120 vs. TAIR10
Match: AT3G63110.1 (AT3G63110.1 isopentenyltransferase 3)

HSP 1 Score: 310.1 bits (793), Expect = 1.4e-84
Identity = 157/286 (54.90%), Postives = 213/286 (74.48%), Query Frame = 1


Query: 4   KEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHH 63
           K+KVV+++G TGTGKSRLS+DIATRF +E+INSDK+QV+ GLDIVTNKIT +E CGVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 64  LLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALID---SNFRLRYE 123
           LLG++ P  D +A+N+  MA LS++++ +  +LPII GGSNSY+EAL+D   + FR RY+
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYD 159

Query: 124 CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPAR-DYSRGIKRAIGVPEFDSY 183
           CCFLWVDV++ VL  FVSERVDKMV +GMV+E  +FFD +  DYSRGIK+AIG PEFD +
Sbjct: 160 CCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRF 219

Query: 184 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 243
           FR    L+ E +E+LL + + ++K +T +LA RQ  KI+RLR VK W+I R+DAT  FTK
Sbjct: 220 FRNEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTK 279

Query: 244 QGK--EADEAWNKHVTIPSTTIIDQFL-----HQNVIATNAAMAAA 279
           +    +A+ AW + V  PST  + +FL      + ++  + A+AAA
Sbjct: 280 RRSKMDANVAWERLVAGPSTDTVSRFLLDIASRRPLVEASTAVAAA 325

[Close]

BLAST of Cucsa.025120 vs. TAIR10
Match: AT3G23630.1 (AT3G23630.1 isopentenyltransferase 7)

HSP 1 Score: 270.0 bits (689), Expect = 1.6e-72
Identity = 137/269 (50.93%), Postives = 196/269 (72.86%), Query Frame = 1


Query: 4   KEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHH 63
           KEKV+ V+G TG+GKSRL+ID+ATRF  E+INSDK+Q+Y GLD++TNK+T +E  GVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 64  LLGIINPFL-DFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALID--SNFRLR-Y 123
           LLG+ +    + +A+ +S +A  ++  +S++++LPI+AGGSNSYIEAL++  S F L  Y
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNY 152

Query: 124 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 183
           +CCF+WVDVS+ VL SFVS+RVD+M+  G+++E  + F+P  +YS GI+RAIGVPE   Y
Sbjct: 153 DCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEY 212

Query: 184 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 243
            R  SL+D   + K+L+ ++  +K++T  LA RQL KIQRL      ++HR+DATE F K
Sbjct: 213 LRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLK 272

Query: 244 QG-KEADEAWNKHVTIPSTTIIDQFLHQN 268
           +  +E DEAW   V  PS  I+D+F + N
Sbjct: 273 RNVEEQDEAWENLVARPSERIVDKFYNNN 301

[Close]

BLAST of Cucsa.025120 vs. TAIR10
Match: AT1G68460.1 (AT1G68460.1 isopentenyltransferase 1)

HSP 1 Score: 235.0 bits (598), Expect = 5.8e-62
Identity = 130/296 (43.92%), Postives = 188/296 (63.51%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           +K+KVV+++G TG GKSRLS+D+ATRFPSE+INSDK+QVY GL+I TN+IT Q++ GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 63  HLLGIINP-FLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNF------ 122
           HLLG+INP   + +A  F   A   +  I+S  ++PIIAGGSNS++ AL+   F      
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 123 ----------RLRYECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPAR---D 182
                      LRYECCF+WVDVS  VL  ++  RVD+M+ +GM +E  +F+DP +   +
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 183 YSRGIKRAIGVPEFDSYFR----YRSLLDEEAQEK-LLEESIAKVKEHTCKLALRQLGKI 242
              GI++AIGVPEFD YF+     + ++  +A  K   ++++  +K +T  LA RQ+ KI
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 243 QRLRNVKGWNIHRLDATEAF------TKQGKEADEAWNKHVTIPSTTIIDQFLHQN 268
           + L++  GW I R+DAT +F      +   K+  E W + V  PS  I+ + L QN
Sbjct: 303 EMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

[Close]

BLAST of Cucsa.025120 vs. TAIR10
Match: AT3G19160.1 (AT3G19160.1 ATP/ADP isopentenyltransferases)

HSP 1 Score: 225.7 bits (574), Expect = 3.5e-59
Identity = 126/291 (43.30%), Postives = 183/291 (62.89%), Query Frame = 1


Query: 4   KEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHH 63
           K+KVV+++G TG+GKS LSID+ATRF  E++NSDK+Q Y GL + TN+++  E+CGVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 64  LLGIINP-FLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNF------- 123
           LLG + P   + + S F  +A  S+  I++   LPIIAGGSNS+I AL+   F       
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPF 161

Query: 124 --------RLRYECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYS--- 183
                    LRYECCFLWVDVS+ VL  ++S+RVD+M+ +GM +E   F+DP    S   
Sbjct: 162 SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 221

Query: 184 -RGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLE----------ESIAKVKEHTCKLALRQ 243
             GI + IG+PEFD YF   SL   E ++K+ E          E++ ++KE+T +LA +Q
Sbjct: 222 AHGIHKTIGIPEFDRYF---SLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQ 281

Query: 244 LGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFL 265
           + +I +L++  GW+I RLDAT +F   G+ + E W+  V   S  ++ +FL
Sbjct: 282 IERIMKLKS-SGWDIQRLDATPSF---GRSSREIWDNTVLDESIKVVKRFL 325

[Close]

BLAST of Cucsa.025120 vs. NCBI nr
Match: gi|449436563|ref|XP_004136062.1| (PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Cucumis sativus])

HSP 1 Score: 545.4 bits (1404), Expect = 5.7e-152
Identity = 279/296 (94.26%), Postives = 280/296 (94.59%), Query Frame = 1


Query: 1   KLQKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGV 60
           KLQKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGV
Sbjct: 35  KLQKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGV 94

Query: 61  PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYE 120
           PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYE
Sbjct: 95  PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYE 154

Query: 121 CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF 180
           CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF
Sbjct: 155 CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF 214

Query: 181 RYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQ 240
           RYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQ
Sbjct: 215 RYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQ 274

Query: 241 GKEADEAWNKHVTIPSTTIIDQFLHQNVIAT----------------NAAMAAATP 281
           GKEADEAWNKHVTIPSTTIIDQFLHQNVIAT                +AAMAAATP
Sbjct: 275 GKEADEAWNKHVTIPSTTIIDQFLHQNVIATKFPTNLASLIGVPRKKSAAMAAATP 330

[Close]

BLAST of Cucsa.025120 vs. NCBI nr
Match: gi|659101009|ref|XP_008451382.1| (PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Cucumis melo])

HSP 1 Score: 523.1 bits (1346), Expect = 3.0e-145
Identity = 267/296 (90.20%), Postives = 274/296 (92.57%), Query Frame = 1


Query: 1   KLQKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGV 60
           KLQKEKVV VIG TGTGKSRLSIDIAT+FPSEVINSDKMQVYHGLDIVTNKITKQEQCGV
Sbjct: 35  KLQKEKVVFVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYHGLDIVTNKITKQEQCGV 94

Query: 61  PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYE 120
           PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRL+YE
Sbjct: 95  PHHLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYE 154

Query: 121 CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF 180
           CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF
Sbjct: 155 CCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYF 214

Query: 181 RYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQ 240
           R RSLLDEEAQEKLLEESIAKVKEHTCKLAL+QLGKIQRLRN+KGWNIHRLDATEAFTKQ
Sbjct: 215 RCRSLLDEEAQEKLLEESIAKVKEHTCKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQ 274

Query: 241 GKEADEAWNKHVTIPSTTIIDQFLHQNVIAT----------------NAAMAAATP 281
           GKEA+EAWNK VTIPSTTIIDQFLH N +AT                +AAMAAATP
Sbjct: 275 GKEAEEAWNKLVTIPSTTIIDQFLHPNAVATKFPTNLASLIGVPRKRSAAMAAATP 330

[Close]

BLAST of Cucsa.025120 vs. NCBI nr
Match: gi|694332220|ref|XP_009356755.1| (PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Pyrus x bretschneideri])

HSP 1 Score: 374.8 bits (961), Expect = 1.3e-100
Identity = 186/289 (64.36%), Postives = 228/289 (78.89%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           QKEKVVIV+G TGTGKSRLSID+AT FP+E+INSDKMQVY GLDIVTNKITK+EQ GVPH
Sbjct: 19  QKEKVVIVMGATGTGKSRLSIDLATCFPAEIINSDKMQVYEGLDIVTNKITKEEQRGVPH 78

Query: 63  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSN---FRLRY 122
           HLLGI++P  DF+A +F D+  L+++++   DRLPII GGSNSYIEAL+D +   FR +Y
Sbjct: 79  HLLGILDPNEDFTARDFCDLTSLAIESVLGRDRLPIIVGGSNSYIEALVDDHDYKFRSKY 138

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 182
           ECCFLWVDVS+ V+ SFVS RVD+MV NGMVDEA +FFDP  DY +GI+RAIGVPEFD Y
Sbjct: 139 ECCFLWVDVSVPVMHSFVSGRVDRMVQNGMVDEAREFFDPNADYMKGIRRAIGVPEFDKY 198

Query: 183 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 242
           FRY  LLDEE + +LL++++ ++K++TCKLA RQL KI RLRN+KGWN+H LDATE F K
Sbjct: 199 FRYGPLLDEETRARLLQQAVDEIKDNTCKLACRQLEKIHRLRNIKGWNLHPLDATEVFRK 258

Query: 243 QGKEADEAWNKHVTIPSTTIIDQFL---------HQNVIATNAAMAAAT 280
           +GKEADEAW K V+ P   I+ QFL         H   +   AA+AAA+
Sbjct: 259 RGKEADEAWEKLVSGPGAMIVGQFLYNMTAEVPPHIGSLRVPAALAAAS 307

[Close]

BLAST of Cucsa.025120 vs. NCBI nr
Match: gi|666875859|ref|NP_001288042.1| (uncharacterized protein LOC103449027 [Malus domestica])

HSP 1 Score: 370.5 bits (950), Expect = 2.5e-99
Identity = 186/289 (64.36%), Postives = 225/289 (77.85%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           QKEKVVIV+G TGTGKSRLSID+AT FP+E+INSDKMQVY GLDIVTNKITK+EQ GVPH
Sbjct: 19  QKEKVVIVMGATGTGKSRLSIDLATCFPAEIINSDKMQVYEGLDIVTNKITKEEQRGVPH 78

Query: 63  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSN---FRLRY 122
           HLLGI++P  DF+A +F D+  L+++++   DRLPII GGSNSYIEAL+D +   FR +Y
Sbjct: 79  HLLGILDPNEDFTARDFCDLTSLAIESVLGRDRLPIIVGGSNSYIEALVDDHDYKFRSKY 138

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 182
           ECCFLWVDVS+ V+ SFVS RVD MV NGMVDEA +FFDP  DY +GI+RAIGVPEFD Y
Sbjct: 139 ECCFLWVDVSVPVMHSFVSGRVDHMVQNGMVDEAREFFDPNADYMKGIRRAIGVPEFDKY 198

Query: 183 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 242
           FRY  LLDEE + +LL++++ K+K++TCKLA RQL KI  LRN KGWN+H LDATE F K
Sbjct: 199 FRYGPLLDEETRARLLQQAVDKIKDNTCKLACRQLEKIHGLRNNKGWNLHPLDATEVFRK 258

Query: 243 QGKEADEAWNKHVTIPSTTIIDQFL---------HQNVIATNAAMAAAT 280
           +GK+ADEAW K V+ P   I+ QFL         H   +   AA+AAAT
Sbjct: 259 RGKDADEAWEKLVSGPGAMIVGQFLYNMTAEVPPHIGSLRVPAALAAAT 307

[Close]

BLAST of Cucsa.025120 vs. NCBI nr
Match: gi|315143145|gb|ADT82707.1| (adenylate isopentenyltransferase [Malus domestica])

HSP 1 Score: 366.3 bits (939), Expect = 4.7e-98
Identity = 183/289 (63.32%), Postives = 224/289 (77.51%), Query Frame = 1


Query: 3   QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 62
           QKEKVVIV+G TGTGKSRLSID+AT FP+E+INSDKMQVY GLDIVTNKITK+EQ GVPH
Sbjct: 31  QKEKVVIVMGATGTGKSRLSIDLATCFPAEIINSDKMQVYEGLDIVTNKITKEEQRGVPH 90

Query: 63  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDS---NFRLRY 122
           HLLG+++P  DF+A +F D+  +++++I   DRLPII GGSNSYIEALID     FR +Y
Sbjct: 91  HLLGMLDPHEDFTARDFCDLTSIAIESILGRDRLPIIVGGSNSYIEALIDDYDYKFRSKY 150

Query: 123 ECCFLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 182
           +CCFLWVDVS  VL SFVS+RVD MV NGMVDEA ++FDP  DY++GI+RAIGVPEFD Y
Sbjct: 151 DCCFLWVDVSTPVLHSFVSKRVDHMVHNGMVDEAREYFDPNADYTKGIRRAIGVPEFDKY 210

Query: 183 FRYRSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTK 242
           FRY   LDEE + +LL +++ ++K++TCKLA RQL KI RLRN+KGWN+H LDATE F K
Sbjct: 211 FRYGPFLDEETRTRLLRQAVDEIKDNTCKLACRQLEKIHRLRNIKGWNLHPLDATEVFRK 270

Query: 243 QGKEADEAWNKHVTIPSTTIIDQFL---------HQNVIATNAAMAAAT 280
           +G+EADEAW K V+  S  I+ QFL         H   +    A+AAAT
Sbjct: 271 RGEEADEAWKKLVSGTSAMIVGQFLYNMTAEVPPHLGSLRVLEALAAAT 319

The following BLAST results are available for this feature:

BLAST of Cucsa.025120 vs. Swiss-Prot


Match Name	E-value	Identity	Description
IPT5_ARATH	5.1e-84	59.55	Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana GN=IPT...	[more]
IPT3_ARATH	2.5e-83	54.90	Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana GN=IPT...	[more]
IPT7_ARATH	2.9e-71	50.93	Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana GN=IPT...	[more]
IPT_HUMLU	5.5e-62	43.56	Adenylate isopentenyltransferase OS=Humulus lupulus PE=1 SV=1	[more]
IPT1_ARATH	1.0e-60	43.92	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana GN=IPT...	[more]

BLAST of Cucsa.025120 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K919_CUCSA	4.0e-152	94.26	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G392940 PE=4 SV=1	[more]
F6KQG8_MALDO	1.8e-99	64.36	Adenylate isopentenyltransferase OS=Malus domestica GN=IPT3b PE=2 SV=1	[more]
F6KSY3_MALDO	3.3e-98	63.32	Adenylate isopentenyltransferase OS=Malus domestica GN=IPT3a PE=2 SV=1	[more]
F6KQG7_MALDO	7.4e-98	62.98	Adenylate isopentenyltransferase OS=Malus domestica GN=IPT3a PE=2 SV=1	[more]
M5VLB1_PRUPE	1.3e-97	63.48	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023171mg PE=3 SV=1	[more]

BLAST of Cucsa.025120 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19040.1	2.9e-85	59.55	isopentenyltransferase 5	[more]
AT3G63110.1	1.4e-84	54.90	isopentenyltransferase 3	[more]
AT3G23630.1	1.6e-72	50.93	isopentenyltransferase 7	[more]
AT1G68460.1	5.8e-62	43.92	isopentenyltransferase 1	[more]
AT3G19160.1	3.5e-59	43.30	ATP/ADP isopentenyltransferases	[more]

BLAST of Cucsa.025120 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449436563\|ref\|XP_004136062.1\|	5.7e-152	94.26	PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Cucumis sativus]	[more]
gi\|659101009\|ref\|XP_008451382.1\|	3.0e-145	90.20	PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Cucumis melo]	[more]
gi\|694332220\|ref\|XP_009356755.1\|	1.3e-100	64.36	PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Pyrus x bretschnei...	[more]
gi\|666875859\|ref\|NP_001288042.1\|	2.5e-99	64.36	uncharacterized protein LOC103449027 [Malus domestica]	[more]
gi\|315143145\|gb\|ADT82707.1\|	4.7e-98	63.32	adenylate isopentenyltransferase [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR018022	IPT
IPR027417	P-loop_NTPase

Vocabulary: **Biological Process**
Term	Definition
GO:0008033	tRNA processing

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009691	cytokinin biosynthetic process
biological_process	GO:0007131	reciprocal meiotic recombination
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005634	nucleus
cellular_component	GO:0009536	plastid
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0052623	ADP dimethylallyltransferase activity
molecular_function	GO:0009824	AMP dimethylallyltransferase activity
molecular_function	GO:0052622	ATP dimethylallyltransferase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.025120.1	Cucsa.025120.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018022	tRNA dimethylallyltransferase	PFAM	PF01715	IPPT	coord: 121..214 score: 1.3E-10coord: 40..113 score: 8.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	G3DSA:3.40.50.300		coord: 3..119 score: 3.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	unknown	SSF52540	P-loop containing nucleoside triphosphate hydrolases	coord: 3..157 score: 7.0
None	No IPR available	GENE3D	G3DSA:1.10.287.890		coord: 131..214 score: 1.1
None	No IPR available	PANTHER	PTHR11088	TRNA DELTA 2 -ISOPENTENYLPYROPHOSPHATE TRANSFERASE-RELATED	coord: 3..277 score: 3.9E
None	No IPR available	PANTHER	PTHR11088:SF30	ADENYLATE ISOPENTENYLTRANSFERASE 3, CHLOROPLASTIC	coord: 3..277 score: 3.9E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.025120	Csa7G392940	Cucumber (Chinese Long) v2	cgycuB519
Cucsa.025120	CSPI07G16890	Wild cucumber (PI 183967)	cgycpiB548
Cucsa.025120	CsaV3_7G028650	Cucumber (Chinese Long) v3	cgycucB563
Cucsa.025120	Carg22191	Silver-seed gourd	carcgyB0981

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None