Cucsa.022170 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.022170
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	NADH-cytochrome b5 reductase
Location	scaffold00313 : 59125 .. 65208 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATACCGGACTCAGGTTCACCTAACTCTGTGGGCACTCCATATTTCTCCGGTGGTTGTAGCTGCCGGAACCGGAAAAAGTTTGGTGAATCAAAACCCATTATAAAATTCTTCATTTTCCACTCAAAGCTCGAAACCCCATTTGATTTTTTTTtGGGAATAAGAATCGTGTGATGGACTTCTTACAGTCTTTGGATCCTCAAATCCTCCTGGGTGTCGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCCTCCAAAAAAAGCAAATCTAGAGGTTACCCACCCCTTGTATTCAAGTTTTCTTTTTAATAATTTATTTATTACCCCATTGTTTGGATTTCCTTGTTGGGTGTTTCGGATTCATGTTTAGAGGTTGAAATTTCTTGTACTTGATTTCTGTTACCAGTTTCTGGGATCTGTTGTGTGTTAATGCAGCGTGAAGTTCTTTCATGTTATTTTGTGAATATGTGGATTTTGTGTTTGATCCATGCAAACTGCTTATATGTATATTTTTGTTGTTGTTTCTATATTATCTTCTCTATTTTTAGAATATTATGTTACAGATAATAAAAAGTAATTTCAGCATACAAGAAGTGAGTTGTTATGCTGACCACTTGAGTTTAATCTGTGTGCTTAAGTTTCCTTATGTTAGTTTTACACTTTCTTGAAGTAAGCAAGTAATATTTTGACCCTCTCTAGATAGTCCCTGGAGTCTGGGTATTACTACAGTTTCATTTTCTTCTTCTGGAACCTGTTTTGTTTTTAAATAAGAGAATCTAATTAATTAACCTTCCCTTTTtATTTGGGGAATATTACAAATAAAGGGAAGAGAGATGATCTTTCTTACAACTCATGAATAGAGTTATATTATATGCTGACTGAGGTTGAGCTTTTATTTTTAGTAGCTTACACTTTGTTGAAGTTGTTGAAGCTAATTACATTTTGACCCTCTAGATAGTTTCTGGAGTCTGAACGTTACTATGGTTCCATTTTCTCTGCTACATTTCAGTATGAACTAAACCAACAATCTGTTTCAAATTGCTCTATTGGTTAAGGAGATTTGATGGGAAATATCACATGAATAGGGATTCACATTTGAGTTACCTGTGAAAATATTTATATGATTTGATAATAAGAAGATTAATTCAGAAAAGTGTGGACCAAGGTTGTGTTTGGTTTATATTTTCAAGTATTTAATTTTGAAAATAAGTCATTTTGGAAAAAATTGAAGTGCTTGGCAATCACTTAAAATAGCTTTTGAAACACCTTTAAAGTTTACTTTGCATAGTTTTTATCAAAAGAGTTAAAATGAAATAATATATTTTTCGAAAACACTTTTTTCCATAGTCAGAGGAAATGGGACCCAAATTTAGTATTCATTCAACCTTTTGCAGGATTCTTGTGCTATGGATGGTTTTGTTGTGATGATACGTGAAAACACAGCCTGTTATTGCTACTTTTTTtCTTCTTCATTTTCTTCATTTTTTTTATCCTTATCATATGATTCAAACATTCAGGTTGCTTGGATCCAGAGATTTTCAAGGAGTTTAAACTTATCAAGCGACTGCAACTGAGCCATAATGTGGCAAAGTTTACATTTACACTCCCTAGTCCTACTTCAGTTCTAGGTCTTCCCATTGGACAACATATAAGTTGCAGGTCTGGCGGTTTGATCCTTATTTATTGTTCATAGGATTAGTTGGATTTCCACATGTCATGCCTATGGGTATGTAGCTGAACAAAAACCTAGATGTTCTGAATATGTTGGTTCTTACACTATATGGTGTGAGAATCTCAATACTATTCTCTTCTGTGTTAACTCTGTTAAAATTAAAGGAAACCTAGAAATACAGCAATTTTCCTTTAAAGATACCTGTTAAACTATCAATCCTGCCACTTCCTCATAATAGATTTTCTGATTTAGCTCATTGGCATTCACAACTTTTATTGGAATAAGGTCATTTAACTTAACGGTTGTTACTTCTTTTGGTCGTTTCGCTTGAGAGAGGTTGTAGGACTTTAGAGGAAACTTTTACGAACTTTGTCGAGGTGTAGTATCTTTTTTTtCTTTGAGCTGATCTATTATGTTGTTCGTTCAAAGACTATTCTAATAATTTGCATCTTCTGAAGTAAACCAACTGGAAATGGTTTTCGGAATCTCCCTTGGGTTTGAGTGGGGATGAATGCCTTATTGCCCCTTTTCTTGGTGTTTTTGAAGATGATTCGCTTCATATGAGAAAAGGATTTATTTCCCTGAGGAAACTTATAATTCATTTATCCTACAGTGGAAAGGATGGCCAAGGTGAAGAGGTTATCAAACCGTACACGCCTATTACTTTGGATTCTGATGTTGGATATTTTGAATTGGTCATAAAGGTTCGTATTTTGGGATTCTCAGTTTGTGAGGAGTATTTTAATGGCATGTATGCATGTTAATCCTAACTCTGGATTGTGTTTTACCAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAGATGCGTGTAGGAGATTTTCTTGCTGTAAAGGGTCCCAAGGTACGTGTAAATCAGTAACTTTTTTTtGGTAAAAGAAACATTTCATTAATGAATGAAATCAGGGGAGAACTCCAAATGCCTAAAGATGATTACAAAAAGAGTGTCAATTAAAAAGAGAGAAAGGGTGCTTTGTTTTACACCATGAAAAAGCCTTGGACAAAACGAACCATAAAACAATCAAAAGAAAAAGAGTTGCTGAAAAGCCTTTTAGTTGTTTTGCCCCATAAACTCCAAAGGAGATTATGCATTAGAGCCAATCAGATCGTCTTCTTTGTACCACCAAAAGGATGACACACCAACAATGATCCAAAGATGTTGTAGATGGAATTTTGTATTAGTAATGGCAGCAATTTAAGTAATGAATTTTTATGCTTTTAAGTAGTGTTTTGTACAAGTTTAAACAGGCACAGCCTTTTAGATATTATACACCATTTTATAGATTATAGTCTATCTTCTTTGATCTAATTGGAGATTTTTTTtGTAATTCACCTCTTGGTGTTACCCAAGCTCCTGATGAAATTTATTTTtCTTGAAAAAAaTAACGTTTTCGTTCATTAACAAATGGGGTAATTACTACTTTGTCCAAATTTGCCCTTTACATGTTACATTGCATAAATATGAACTCAGATTTCAGGTTCTGCGAGTTCAAATCCATTCATGTTTAACAAGTTAATTGGAACATTAATACATTTGAGCTAAATGGAAGGATTCTCCCTTTTTATAGTTTATCTTTTTtAATTATTTTTTtCAAAATATCTTATGAATAAATTGTTGACTCAATATGTTTGCAGCCTTTTCTTGATCAGTGATTTCTACAACAGTTCTAACAGTTCTTATAAAAAAaTAACAGTTTGAACAGTAAATTAAAAACAAAGAAAGATTTTAGTGAATATGTGTAATTTTAAACAGAGAACTGAAAGATTAGTCATATTGAAGTTGATATGGAAATTAGTTTCCCATGCCAGCTGCCTTAATTTTTGTTATAAGTTCTGTCTACCATTGAGCTTCCTTTTGGGTTAGCCACAGACTTCTCCCCCACGTTCTATTTCGAGAATTTATTGACAATGTTTTGTTTGTCACACACACAAAAAaCAAATCCCATTTTCTCTCTGAGATAGAGAGATGAATAAAAAACTTCACAGTACCTTTAAAGACACCGAGAATATTCAAAATCTACAATGAAAACATTCATGCAGGGACGTTTCAGATATCAACCTGGCCAGGTCAGAGCATTTGGTATGCTTGCTGGAGGCTCTGGCATTACTCCTATGTATCAAGTACAGATTTCCTTTTCTTTTGTCTCCATCATTTTAGTTCATCAATTTACCATCATTTGGACTTCTTGATTGTTGTTATAGATATTTGGCTTTTGTTTCTGCTCCTTAGGTTGCTCGGGCAATTTTAGAAAATCCAAACGACAAGACAAAGGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTCTTAAAGGTATTTTCGTTGTCTTTCTTCAATAAAATTCCCTCTCGCCCTCATGTTGATCAATTCATAAATTTGGATACTAGGCTATCTTTTtATTTTATAACATGTTGATAGCTAGTTTTTCATAACATGTCTGAGTGATATTGTAATCAGTCTACCCCTTTCTCAGTGCTGTGAATTTTAACGGTTGAATGTTTGAACTCATTAGTCGAACACTTGACTAGAATTACATTTGTCAACTCAAGCATAGTGTAACTAGTTAAAGACATATTTTCAACCATAAAGGTTTTGTCATTTTTACCTGGATAAGAAAGGCATGCAAATTTGTGCTATTTTGGGTATTAAAAGGTGATTTGATTGAAAGGAAACAGAAGACAACAACTTCTTGGGCAAAATTGTAGGATCTTAATACTCTTGTAGCCTATTCTTAGACTTCCACTTTTGCATTCATTATAATAATTCAATTTCTATGATTTCTCTCTCTCTAATTTCGTCAAGTGTTCCTTACCAAAAGAAACTCGTGTGTTTATAGGAGGAGTTGGACCTTCTTGCCAAAAGGTACCCCGATAGCTTCAAGCTCTACTATGTCTTAAACCAGGTCAGTCTTCTTCCTCAGCCACAGCGACAGCCACAAATTTAAACTGTTCTCTATGGCTAAACTTTATAGCATTACAAGATCTCCATGTCATCGTATTATGGTTGTGCTGTTTTGCTTTACTGACAGATATTACGTGATATATGCGAACTTTGACAAATCTAGTGAATTAAATTGGATTTCTAGTTCTTCAAGTCTGGGGTTGTTTGATAACCGCTCTTAATAGTTGTTTTCGATTTCTTAAAACAAAAaGGTAAATGAAAATCCTTTTAGTTAACTGTTTTCTAAAAGCTGTTTATTTTTGTGGGCGATGTATCTCTCAAGAGCCACCCCAATTACAATTCTTAAAAACAGTTTCGCGAAAGAAGGTACCAAGCACCTGCAGAGTTGTAAAGCTGTGTGTTATGGATGGAATTAGCTTCTCTTAGGCCATGATCGATCTTCTCTTGAGCTTCATTTAAGCTTGCTTACACATTTACTTTTGTTGAATGATTTGCGATGTCATATCTTTTTtATCTCGTGTTCCAATTGCTTAATCAGCCTCCTGAAGCGTGGGATGGCGGCGTTGGATTTGTATCGAAGGAGATGATTCAAACCCACTGTCCTGCTCCTGCTTCTGATATTCAGGTATATTTGATTATTAACTCAAACCATATTTCTTGTTTTCTTGTAACTAATAAACCAAAAGGCTCTCTCTTGTTTTCTTGTAATTTGGAAAAAGCAACTGAAGTTTTTTTTtCTTTGTAAACAAGCCCTTTTCACTCATATTATCAAAAAGACTAATTAAGAAAAAAAAaCATATAATTACAACTAATATAGTACATAATTAAACTGGAAAAATAAAAGCGGTCGTGATGTTGGATCAGAATATATTTTTTAAAATTGTGATTGTTTAATCTAAAAAACTCATAAGTAAAAGCAATGCTTATTAGCTCATTTTAGGGACCTAACTCAATTATAGTAGTTTTCTATCTAACCTTTTATTTAGGAAGAACCCGTACTTCAACTTCATCAAACTTGGAAAATATTGTCTCATATGAAAACTTATTTTGTAAGCAGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGCTGCTCATCTTGAAGAACTTGGATATGCACCTGAGATGCTGTTCATGTTCTGATCCTTCCTCAAGTTCTTCAGAAGAGCTAAGCGGAATTTTCCCCCTTCTAAATTTTGAAGATTTCAAAATTCTGTCTTATTTTTTtAACTAAAAATTTATGCACTGAAGAGGTTTTCCTTTTAGTCACTTTTCATTTAACAATGTTGTGGAATTATGATGCCATCAAATACAGTTACTTCCAACAGTTAGAGCTAGCGAGAATTTAAACTAGTTTTTCTTGAATTGTTTCTTCAGCAAATAACTATGGAATGGTCTCACACTTTGCTGTGCCTATCGAGGAAAGATAACAATAAACATGGTCATCATGTTAGA

mRNA sequence

AAAATACCGGACTCAGGTTCACCTAACTCTGTGGGCACTCCATATTTCTCCGGTGGTTGTAGCTGCCGGAACCGGAAAAAGTTTGGTGAATCAAAACCCATTATAAAATTCTTCATTTTCCACTCAAAGCTCGAAACCCCATTTGATTTTTTTTTGGGAATAAGAATCGTGTGATGGACTTCTTACAGTCTTTGGATCCTCAAATCCTCCTGGGTGTCGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCCTCCAAAAAAAGCAAATCTAGAGGTTGCTTGGATCCAGAGATTTTCAAGGAGTTTAAACTTATCAAGCGACTGCAACTGAGCCATAATGTGGCAAAGTTTACATTTACACTCCCTAGTCCTACTTCAGTTCTAGGTCTTCCCATTGGACAACATATAAGTTGCAGTGGAAAGGATGGCCAAGGTGAAGAGGTTATCAAACCGTACACGCCTATTACTTTGGATTCTGATGTTGGATATTTTGAATTGGTCATAAAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAGATGCGTGTAGGAGATTTTCTTGCTGTAAAGGGTCCCAAGGGACGTTTCAGATATCAACCTGGCCAGGTCAGAGCATTTGGTATGCTTGCTGGAGGCTCTGGCATTACTCCTATGTATCAAGTTGCTCGGGCAATTTTAGAAAATCCAAACGACAAGACAAAGGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTCTTAAAGGAGGAGTTGGACCTTCTTGCCAAAAGGTACCCCGATAGCTTCAAGCTCTACTATGTCTTAAACCAGCCTCCTGAAGCGTGGGATGGCGGCGTTGGATTTGTATCGAAGGAGATGATTCAAACCCACTGTCCTGCTCCTGCTTCTGATATTCAGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGCTGCTCATCTTGAAGAACTTGGATATGCACCTGAGATGCTGTTCATGTTCTGATCCTTCCTCAAGTTCTTCAGAAGAGCTAAGCGGAATTTTCCCCCTTCTAAATTTTGAAGATTTCAAAATTCTGTCTTATTTTTTTAACTAAAAATTTATGCACTGAAGAGGTTTTCCTTTTAGTCACTTTTCATTTAACAATGTTGTGGAATTATGATGCCATCAAATACAGTTACTTCCAACAGTTAGAGCTAGCGAGAATTTAAACTAGTTTTTCTTGAATTGTTTCTTCAGCAAATAACTATGGAATGGTCTCACACTTTGCTGTGCCTATCGAGGAaagataacaataaacatggtcatcatgttaga

Coding sequence (CDS)

ATGGACTTCTTACAGTCTTTGGATCCTCAAATCCTCCTGGGTGTCGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCCTCCAAAAAAAGCAAATCTAGAGGTTGCTTGGATCCAGAGATTTTCAAGGAGTTTAAACTTATCAAGCGACTGCAACTGAGCCATAATGTGGCAAAGTTTACATTTACACTCCCTAGTCCTACTTCAGTTCTAGGTCTTCCCATTGGACAACATATAAGTTGCAGTGGAAAGGATGGCCAAGGTGAAGAGGTTATCAAACCGTACACGCCTATTACTTTGGATTCTGATGTTGGATATTTTGAATTGGTCATAAAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAGATGCGTGTAGGAGATTTTCTTGCTGTAAAGGGTCCCAAGGGACGTTTCAGATATCAACCTGGCCAGGTCAGAGCATTTGGTATGCTTGCTGGAGGCTCTGGCATTACTCCTATGTATCAAGTTGCTCGGGCAATTTTAGAAAATCCAAACGACAAGACAAAGGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTCTTAAAGGAGGAGTTGGACCTTCTTGCCAAAAGGTACCCCGATAGCTTCAAGCTCTACTATGTCTTAAACCAGCCTCCTGAAGCGTGGGATGGCGGCGTTGGATTTGTATCGAAGGAGATGATTCAAACCCACTGTCCTGCTCCTGCTTCTGATATTCAGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGCTGCTCATCTTGAAGAACTTGGATATGCACCTGAGATGCTGTTCATGTTCTGA

Protein sequence

MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF*

Homology

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BLAST of Cucsa.022170 vs. Swiss-Prot
Match: NB5R1_ARATH (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana GN=CBR1 PE=1 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 3.3e-131
Identity = 230/280 (82.14%), Postives = 249/280 (88.93%), Query Frame = 1


Query: 2   DFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHN 61
           +FL++LD QILLGV VA +A+  GA Y L+SSKK   R CLDPE FKEFKL+KR QLSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKKR--RVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQ 121
           VAKF F LP+ TSVLGLPIGQHISC GKDGQGE+VIKPYTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPND 181
           GRMSHHFR+MRVGD LAVKGPKGRF+YQPGQ RAFGMLAGGSGITPM+QVARAILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQT 241
           KTKVHLIYANVTY+DILLKEEL+ L   YP+ FK++YVLNQPPE WDGGVGFVSKEMIQT
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           HCPAPASDIQILRCGPPPMNKAMAA+LE LGY+PEM F F
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

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BLAST of Cucsa.022170 vs. Swiss-Prot
Match: NCB5R_DICDI (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 3.1e-73
Identity = 138/280 (49.29%), Postives = 190/280 (67.86%), Query Frame = 1


Query: 15  VAVAVLALAVGALYVL---SSSKKSKSRG----------CLDPEIFKEFKLIKRLQLSHN 74
           V +  +ALA G  YV    S S K K  G           LDP+ +++F+L ++  ++HN
Sbjct: 9   VIIGSVALAAGVKYVFTLTSGSNKDKKGGEAEKGKQVEKALDPQEYRKFQLKEKFIVNHN 68

Query: 75  VAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQ 134
              F F LP+   +LGLPIGQHIS     G G+EV +PYTPI+ D + GYF+L+IK+Y +
Sbjct: 69  TRIFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYTPISSDEERGYFDLLIKVYEK 128

Query: 135 GRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPND 194
           G MS +   M +GD + VKGPKG+F YQP   ++ GMLAGG+GITPM QV +AIL+NP+D
Sbjct: 129 GAMSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAGGTGITPMLQVIKAILKNPSD 188

Query: 195 KTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQT 254
           KT++ L++ N+T EDILLK+ELD LA+++P  FK+YYVLN PP+ W  GVGFVSKE+I++
Sbjct: 189 KTEISLVFGNITEEDILLKKELDELAEKHP-QFKVYYVLNNPPKGWTQGVGFVSKEIIES 248

Query: 255 HCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
             P+P+    ++ CGPP MNKAM  HLE +G+    +F F
Sbjct: 249 RLPSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

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BLAST of Cucsa.022170 vs. Swiss-Prot
Match: NCB5R_ASPFU (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) GN=cbr1 PE=3 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 1.7e-71
Identity = 136/264 (51.52%), Postives = 180/264 (68.18%), Query Frame = 1


Query: 16  AVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPTSV 75
           AVAV A+  G       +K  K    L+P  F+EF L +++ +SHNV  + F LP PT +
Sbjct: 34  AVAVTAILAGLKLFTGGNKPRK---VLNPTEFQEFVLKEKIDVSHNVCIYRFALPRPTDI 93

Query: 76  LGLPIGQHISCSGK-DGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVG 135
           LGLPIGQHIS +   +GQ +EV++ YTPI+ D++ GYF+L++K YPQG +S +   +++G
Sbjct: 94  LGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIG 153

Query: 136 DFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILEN-----PNDKTKVHLIY 195
           D + V+GPKG   Y P   R  GM+AGG+GITPM Q+ +A++ N      ND TK+ LI+
Sbjct: 154 DTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIF 213

Query: 196 ANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASD 255
           ANV  +DILLKEELD+LA   PD F +YYVLN PP+ W GGVGFV+ EMI+ H PAPASD
Sbjct: 214 ANVNPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASD 273

Query: 256 IQILRCGPPPMNKAMAAHLEELGY 274
           ++IL CGPPPM  AM    E LGY
Sbjct: 274 VKILLCGPPPMISAMKKATESLGY 293

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BLAST of Cucsa.022170 vs. Swiss-Prot
Match: NCB5R_PICGU (NADH-cytochrome b5 reductase 1 OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) GN=CBR1 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 8.5e-71
Identity = 135/266 (50.75%), Postives = 179/266 (67.29%), Query Frame = 1


Query: 8   DPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTF 67
           +P ++     A++  AV A+YV+  +KK+     L P++F++F LI++ +LSHN   + F
Sbjct: 7   NPLVIFSTLAAIILAAV-AVYVVKLNKKNGP--VLKPDVFQKFPLIEKTRLSHNTCIYRF 66

Query: 68  TLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHH 127
            LP  T  LGLPIGQHIS  G    G+EV++ YTPI+ D ++GYF+L+IK Y QG +S H
Sbjct: 67  GLPKSTDRLGLPIGQHISI-GATINGKEVVRSYTPISRDDELGYFDLLIKTYEQGNISRH 126

Query: 128 FRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHL 187
                VGD + V+GPKG F Y P  V   GM+AGG+GI PMYQV  AIL NP+DKTK+ L
Sbjct: 127 VDSKSVGDHIEVRGPKGFFTYTPNMVEHLGMIAGGTGIAPMYQVLTAILTNPDDKTKISL 186

Query: 188 IYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPA 247
           +YANVT EDILL+ ELDL AK +PD FK++YVLN  PE W+G VGFV+ E+++ H P   
Sbjct: 187 VYANVTEEDILLRAELDLFAKEHPDRFKVHYVLNNAPENWNGSVGFVTPEIMEKHLPNKD 246

Query: 248 SDIQILRCGPPPMNKAMAAHLEELGY 274
            D  +L CGPPPM  AM  +   LGY
Sbjct: 247 QDGYLLLCGPPPMISAMKKNAVTLGY 268

[Close]

BLAST of Cucsa.022170 vs. Swiss-Prot
Match: NCB5R_ASPOR (NADH-cytochrome b5 reductase 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) GN=cbr1 PE=3 SV=2)

HSP 1 Score: 267.3 bits (682), Expect = 1.9e-70
Identity = 137/268 (51.12%), Postives = 182/268 (67.91%), Query Frame = 1


Query: 12  LLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTFTLPS 71
           LL V  A+ A+ VGA ++  S K  K    L+P  F+ F L ++ ++SHNVA + F LP 
Sbjct: 13  LLVVGTAIFAVLVGAKFLGGSGKPRK---VLNPTEFQNFVLKEKNEISHNVAIYRFALPR 72

Query: 72  PTSVLGLPIGQHISCSGK-DGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQ 131
           PT +LGLPIGQHIS +   +GQ +EV++ YTPI+ D++ GYF+L++K YPQG +S +   
Sbjct: 73  PTDILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTT 132

Query: 132 MRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILEN-----PNDKTKV 191
           +++GD + V+GPKG   Y P   R  GM+AGG+GITPM Q+ +AI+ N      ND T+V
Sbjct: 133 LKIGDNMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAIIRNRPRNGGNDTTQV 192

Query: 192 HLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPA 251
            LI+ANV  EDILLKEEL+ L K   D F++YYVLN PPE W GGVGFV+ +MI+   PA
Sbjct: 193 DLIFANVNPEDILLKEELEQLVKE-DDGFRVYYVLNNPPEGWTGGVGFVTPDMIKERLPA 252

Query: 252 PASDIQILRCGPPPMNKAMAAHLEELGY 274
           PA DI+I+ CGPPPM  AM    E LGY
Sbjct: 253 PAQDIKIMLCGPPPMISAMKKATESLGY 276

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BLAST of Cucsa.022170 vs. TrEMBL
Match: A0A0A0L226_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G416480 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 2.0e-159
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH
Sbjct: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of Cucsa.022170 vs. TrEMBL
Match: A0A151TAA8_CAJCA (NADH-cytochrome b5 reductase OS=Cajanus cajan GN=KK1_018575 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.2e-134
Identity = 234/281 (83.27%), Postives = 257/281 (91.46%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQ+ + QIL+GVAVAV+A+ VGA+Y+ SS K++   GCLDPE FKEFKL+KR QLSH
Sbjct: 1   MDFLQAPENQILVGVAVAVVAIGVGAVYLYSSKKRN---GCLDPEDFKEFKLVKRAQLSH 60

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTF LP+PTSVLGLPIGQHISC GKD QGEEVIKPYTP TLDSDVG+FELVIKMYP
Sbjct: 61  NVAKFTFALPTPTSVLGLPIGQHISCRGKDAQGEEVIKPYTPTTLDSDVGHFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFR+MRVGD+LAVKGPKGRF+YQPG+VRAFGMLAGGSGITPM+QVARAILENPN
Sbjct: 121 QGRMSHHFREMRVGDYLAVKGPKGRFKYQPGEVRAFGMLAGGSGITPMFQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANV YEDILLKEE+D LA  YPD FK+YYVLNQPPE W+GGVGFVSKEMIQ
Sbjct: 181 DRTKVHLIYANVMYEDILLKEEIDGLASNYPDRFKVYYVLNQPPEVWNGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPA DI+ILRCGPPPMNKAMAAHLE +GYAPEM F F
Sbjct: 241 THCPAPAHDIKILRCGPPPMNKAMAAHLEAIGYAPEMQFQF 278

[Close]

BLAST of Cucsa.022170 vs. TrEMBL
Match: W9QYB4_9ROSA (NADH-cytochrome b5 reductase OS=Morus notabilis GN=L484_020174 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 1.4e-133
Identity = 229/281 (81.49%), Postives = 255/281 (90.75%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           M+FL+S+DPQIL+G AVA++A+ VGA ++ SS K   ++GCLDPE FKEFKL+KR QLSH
Sbjct: 1   MEFLESVDPQILVGAAVAIVAIGVGAAFLFSSRK---AKGCLDPENFKEFKLVKRTQLSH 60

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTF LP+PTS+LGLPIGQHISC GKD QGEEVIKPYTP TLDSDVG+FELVIKMYP
Sbjct: 61  NVAKFTFELPTPTSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGHFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFR+MRVGD+LAVKGPKGRFRY PGQVRAFGMLAGGSGITPMYQVARAILENP 
Sbjct: 121 QGRMSHHFREMRVGDYLAVKGPKGRFRYTPGQVRAFGMLAGGSGITPMYQVARAILENPK 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELD LA  YPD FK+Y+VLNQPPE W+GGVGFVSKE+IQ
Sbjct: 181 DQTKVHLIYANVTYEDILLKEELDGLATNYPDQFKVYFVLNQPPEGWNGGVGFVSKEIIQ 240

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPA D+Q+LRCGPPPMNKAMA HL ++GYAPEM F F
Sbjct: 241 THCPAPAPDVQVLRCGPPPMNKAMAGHLNDIGYAPEMQFQF 278

[Close]

BLAST of Cucsa.022170 vs. TrEMBL
Match: V4VDL5_9ROSI (NADH-cytochrome b5 reductase OS=Citrus clementina GN=CICLE_v10032349mg PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 1.4e-133
Identity = 236/281 (83.99%), Postives = 254/281 (90.39%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           M++LQ+LD Q+L+G AVAVLA+AVGA Y+ SS K   S   LDPE FKEFKL+KRLQLSH
Sbjct: 3   MEYLQNLDVQLLVGGAVAVLAIAVGAAYLYSSKKPKVS---LDPENFKEFKLVKRLQLSH 62

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTF LP+PTSVLGLPIGQHISC GKD QGEEVIKPYTP TLDSDVG+FELVIKMYP
Sbjct: 63  NVAKFTFELPTPTSVLGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGHFELVIKMYP 122

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFR+MRVGD+LAVKGPKGRFRYQPGQVRAFGM AGGSGITPM+QV RAILENPN
Sbjct: 123 QGRMSHHFREMRVGDYLAVKGPKGRFRYQPGQVRAFGMTAGGSGITPMFQVTRAILENPN 182

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELD  A +YPD F +YYVLNQPPE+W+GGVGFVSKEMIQ
Sbjct: 183 DKTKVHLIYANVTYEDILLKEELDGFAAKYPDQFTIYYVLNQPPESWNGGVGFVSKEMIQ 242

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDIQ+LRCGPPPMNKAMAAHLE LGY  EMLF F
Sbjct: 243 THCPAPASDIQVLRCGPPPMNKAMAAHLEALGYTSEMLFQF 280

[Close]

BLAST of Cucsa.022170 vs. TrEMBL
Match: A0A0B2S646_GLYSO (NADH-cytochrome b5 reductase OS=Glycine soja GN=glysoja_021666 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 1.9e-133
Identity = 233/281 (82.92%), Postives = 255/281 (90.75%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQ+ + QIL+GVAVAV+A+ +GA+Y+ SS K   ++GCLDPE FK FKL+KR QLSH
Sbjct: 1   MDFLQAQENQILVGVAVAVVAIGLGAVYLYSSKK---TKGCLDPENFKAFKLVKRAQLSH 60

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTF LP+PTSVLGLPIGQHISC GKD QGEEVIKPYTP TLDSDVG+FELVIKMYP
Sbjct: 61  NVAKFTFALPTPTSVLGLPIGQHISCRGKDAQGEEVIKPYTPTTLDSDVGHFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFR+MRVGD+L+VKGPKGRF+YQPG+VRAFGMLAGGSGITPM+QVARAILENPN
Sbjct: 121 QGRMSHHFREMRVGDYLSVKGPKGRFKYQPGEVRAFGMLAGGSGITPMFQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELD LA  YPD FK+YYVLNQPPE WDGG GFVSKEMIQ
Sbjct: 181 DRTKVHLIYANVTYEDILLKEELDGLASNYPDRFKIYYVLNQPPEVWDGGEGFVSKEMIQ 240

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPA DI+ILRCGPPPMNKAMAAHLE LGYA EM F F
Sbjct: 241 THCPAPAQDIKILRCGPPPMNKAMAAHLEALGYASEMQFQF 278

[Close]

BLAST of Cucsa.022170 vs. TAIR10
Match: AT5G17770.1 (AT5G17770.1 NADH:cytochrome B5 reductase 1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.8e-132
Identity = 230/280 (82.14%), Postives = 249/280 (88.93%), Query Frame = 1


Query: 2   DFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHN 61
           +FL++LD QILLGV VA +A+  GA Y L+SSKK   R CLDPE FKEFKL+KR QLSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKKR--RVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQ 121
           VAKF F LP+ TSVLGLPIGQHISC GKDGQGE+VIKPYTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPND 181
           GRMSHHFR+MRVGD LAVKGPKGRF+YQPGQ RAFGMLAGGSGITPM+QVARAILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQT 241
           KTKVHLIYANVTY+DILLKEEL+ L   YP+ FK++YVLNQPPE WDGGVGFVSKEMIQT
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           HCPAPASDIQILRCGPPPMNKAMAA+LE LGY+PEM F F
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of Cucsa.022170 vs. TAIR10
Match: AT5G20080.1 (AT5G20080.1 FAD/NAD(P)-binding oxidoreductase)

HSP 1 Score: 209.5 bits (532), Expect = 2.6e-54
Identity = 110/273 (40.29%), Postives = 167/273 (61.17%), Query Frame = 1


Query: 27  LYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPTSVLGLPIGQHISC 86
           +Y+  + +++  +  L+P+ + EFKL    ++SHN   F F+   P++ LGL +   +  
Sbjct: 58  VYLDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSF-DPSAELGLHVASCLLT 117

Query: 87  SGK-----DGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFLAVKG 146
                   +G+ + VI+PYTPI+     GYF+L+IK+YP G+MS HF  ++ GD L VKG
Sbjct: 118 RAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKG 177

Query: 147 PKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKE 206
           P  +F+Y P   +  GM+AGGSGITPM QV  AI++NP D T++ L+YANV+ +DILLK+
Sbjct: 178 PVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQ 237

Query: 207 ELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASDIQILRCGPPPMN 266
           +LD+L   +P + K++Y ++ P + W GGVG++SK+M     P P  D  IL CGPP M 
Sbjct: 238 KLDVLQANHP-NLKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMM 297

Query: 267 KAMAAH-------------LEELGYAPEMLFMF 282
           + ++               L+ELGY  EM+F F
Sbjct: 298 EHISGGKAPDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of Cucsa.022170 vs. TAIR10
Match: AT1G37130.1 (AT1G37130.1 nitrate reductase 2)

HSP 1 Score: 171.0 bits (432), Expect = 1.0e-42
Identity = 97/253 (38.34%), Postives = 148/253 (58.50%), Query Frame = 1


Query: 42  LDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYT 101
           ++P      +L+++  +SH+V KF F LP    VLGLP+G+HI         +  ++ YT
Sbjct: 657 VNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRAYT 716

Query: 102 PITLDSDVGYFELVIKMY-----PQ----GRMSHHFRQMRVGDFLAVKGPKGRFRY---- 161
           P +    VGYFELV+K+Y     P+    G MS +   + +G  L +KGP G   Y    
Sbjct: 717 PSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKG 776

Query: 162 ------QPGQVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKEE 221
                 +P       MLAGG+GITP+YQ+ +AIL++P D+T++++IYAN T EDILL+EE
Sbjct: 777 SFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREE 836

Query: 222 LDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASDIQI-LRCGPPPMN 274
           LD  A++YPD  K++YV+    E W    GF+S+ +++ H P       + + CGPPPM 
Sbjct: 837 LDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPPMI 896

[Close]

BLAST of Cucsa.022170 vs. TAIR10
Match: AT1G77760.1 (AT1G77760.1 nitrate reductase 1)

HSP 1 Score: 162.5 bits (410), Expect = 3.7e-40
Identity = 85/244 (34.84%), Postives = 142/244 (58.20%), Query Frame = 1


Query: 51  KLIKRLQLSHNVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVG 110
           +LI++  +SH+V KF F LPS    LGLP+G+H+         +  ++ YTP +    VG
Sbjct: 666 RLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANIND-KLCLRAYTPTSAIDAVG 725

Query: 111 YFELVIKMYPQ---------GRMSHHFRQMRVGDFLAVKGPKGRFRYQ----------PG 170
           + +LV+K+Y +         G MS H   + +G  + +KGP G   Y+          P 
Sbjct: 726 HIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKPK 785

Query: 171 QVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKEELDLLAKRYP 230
             +   MLAGG+GITP+YQ+ ++IL +P D+T+++++YAN T +DIL++EEL+  A ++ 
Sbjct: 786 FAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEGWASKHK 845

Query: 231 DSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASDIQI-LRCGPPPMNK-AMAAHLE 274
           +  K++YV+    E W    GF+++ +++ H P       + L CGPPPM + A+  +LE
Sbjct: 846 ERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQFALQPNLE 905

[Close]

BLAST of Cucsa.022170 vs. NCBI nr
Match: gi|449453115|ref|XP_004144304.1| (PREDICTED: NADH--cytochrome b5 reductase 1 [Cucumis sativus])

HSP 1 Score: 569.7 bits (1467), Expect = 2.8e-159
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH
Sbjct: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of Cucsa.022170 vs. NCBI nr
Match: gi|659111527|ref|XP_008455773.1| (PREDICTED: NADH--cytochrome b5 reductase 1 [Cucumis melo])

HSP 1 Score: 563.9 bits (1452), Expect = 1.6e-157
Identity = 277/281 (98.58%), Postives = 279/281 (99.29%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQILLG+AVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLP+PTSVLGLPIGQHISC GKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of Cucsa.022170 vs. NCBI nr
Match: gi|1012352800|gb|KYP63988.1| (NADH-cytochrome b5 reductase 1 [Cajanus cajan])

HSP 1 Score: 486.5 bits (1251), Expect = 3.2e-134
Identity = 234/281 (83.27%), Postives = 257/281 (91.46%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQ+ + QIL+GVAVAV+A+ VGA+Y+ SS K++   GCLDPE FKEFKL+KR QLSH
Sbjct: 1   MDFLQAPENQILVGVAVAVVAIGVGAVYLYSSKKRN---GCLDPEDFKEFKLVKRAQLSH 60

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTF LP+PTSVLGLPIGQHISC GKD QGEEVIKPYTP TLDSDVG+FELVIKMYP
Sbjct: 61  NVAKFTFALPTPTSVLGLPIGQHISCRGKDAQGEEVIKPYTPTTLDSDVGHFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFR+MRVGD+LAVKGPKGRF+YQPG+VRAFGMLAGGSGITPM+QVARAILENPN
Sbjct: 121 QGRMSHHFREMRVGDYLAVKGPKGRFKYQPGEVRAFGMLAGGSGITPMFQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANV YEDILLKEE+D LA  YPD FK+YYVLNQPPE W+GGVGFVSKEMIQ
Sbjct: 181 DRTKVHLIYANVMYEDILLKEEIDGLASNYPDRFKVYYVLNQPPEVWNGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPA DI+ILRCGPPPMNKAMAAHLE +GYAPEM F F
Sbjct: 241 THCPAPAHDIKILRCGPPPMNKAMAAHLEAIGYAPEMQFQF 278

[Close]

BLAST of Cucsa.022170 vs. NCBI nr
Match: gi|703080251|ref|XP_010091372.1| (NADH-cytochrome b5 reductase 1 [Morus notabilis])

HSP 1 Score: 483.8 bits (1244), Expect = 2.0e-133
Identity = 229/281 (81.49%), Postives = 255/281 (90.75%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           M+FL+S+DPQIL+G AVA++A+ VGA ++ SS K   ++GCLDPE FKEFKL+KR QLSH
Sbjct: 1   MEFLESVDPQILVGAAVAIVAIGVGAAFLFSSRK---AKGCLDPENFKEFKLVKRTQLSH 60

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTF LP+PTS+LGLPIGQHISC GKD QGEEVIKPYTP TLDSDVG+FELVIKMYP
Sbjct: 61  NVAKFTFELPTPTSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGHFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFR+MRVGD+LAVKGPKGRFRY PGQVRAFGMLAGGSGITPMYQVARAILENP 
Sbjct: 121 QGRMSHHFREMRVGDYLAVKGPKGRFRYTPGQVRAFGMLAGGSGITPMYQVARAILENPK 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELD LA  YPD FK+Y+VLNQPPE W+GGVGFVSKE+IQ
Sbjct: 181 DQTKVHLIYANVTYEDILLKEELDGLATNYPDQFKVYFVLNQPPEGWNGGVGFVSKEIIQ 240

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPA D+Q+LRCGPPPMNKAMA HL ++GYAPEM F F
Sbjct: 241 THCPAPAPDVQVLRCGPPPMNKAMAGHLNDIGYAPEMQFQF 278

[Close]

BLAST of Cucsa.022170 vs. NCBI nr
Match: gi|567889474|ref|XP_006437259.1| (hypothetical protein CICLE_v10032349mg [Citrus clementina])

HSP 1 Score: 483.8 bits (1244), Expect = 2.0e-133
Identity = 236/281 (83.99%), Postives = 254/281 (90.39%), Query Frame = 1


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           M++LQ+LD Q+L+G AVAVLA+AVGA Y+ SS K   S   LDPE FKEFKL+KRLQLSH
Sbjct: 3   MEYLQNLDVQLLVGGAVAVLAIAVGAAYLYSSKKPKVS---LDPENFKEFKLVKRLQLSH 62

Query: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTF LP+PTSVLGLPIGQHISC GKD QGEEVIKPYTP TLDSDVG+FELVIKMYP
Sbjct: 63  NVAKFTFELPTPTSVLGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGHFELVIKMYP 122

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFR+MRVGD+LAVKGPKGRFRYQPGQVRAFGM AGGSGITPM+QV RAILENPN
Sbjct: 123 QGRMSHHFREMRVGDYLAVKGPKGRFRYQPGQVRAFGMTAGGSGITPMFQVTRAILENPN 182

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELD  A +YPD F +YYVLNQPPE+W+GGVGFVSKEMIQ
Sbjct: 183 DKTKVHLIYANVTYEDILLKEELDGFAAKYPDQFTIYYVLNQPPESWNGGVGFVSKEMIQ 242

Query: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDIQ+LRCGPPPMNKAMAAHLE LGY  EMLF F
Sbjct: 243 THCPAPASDIQVLRCGPPPMNKAMAAHLEALGYTSEMLFQF 280

The following BLAST results are available for this feature:

BLAST of Cucsa.022170 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NB5R1_ARATH	3.3e-131	82.14	NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana GN=CBR1 PE=1 SV=1	[more]
NCB5R_DICDI	3.1e-73	49.29	NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum GN=cyb5r1 PE=3 SV=1	[more]
NCB5R_ASPFU	1.7e-71	51.52	NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A...	[more]
NCB5R_PICGU	8.5e-71	50.75	NADH-cytochrome b5 reductase 1 OS=Meyerozyma guilliermondii (strain ATCC 6260 / ...	[more]
NCB5R_ASPOR	1.9e-70	51.12	NADH-cytochrome b5 reductase 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40...	[more]

BLAST of Cucsa.022170 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L226_CUCSA	2.0e-159	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G416480 PE=4 SV=1	[more]
A0A151TAA8_CAJCA	2.2e-134	83.27	NADH-cytochrome b5 reductase OS=Cajanus cajan GN=KK1_018575 PE=3 SV=1	[more]
W9QYB4_9ROSA	1.4e-133	81.49	NADH-cytochrome b5 reductase OS=Morus notabilis GN=L484_020174 PE=3 SV=1	[more]
V4VDL5_9ROSI	1.4e-133	83.99	NADH-cytochrome b5 reductase OS=Citrus clementina GN=CICLE_v10032349mg PE=3 SV=1	[more]
A0A0B2S646_GLYSO	1.9e-133	82.92	NADH-cytochrome b5 reductase OS=Glycine soja GN=glysoja_021666 PE=3 SV=1	[more]

BLAST of Cucsa.022170 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G17770.1	1.8e-132	82.14	NADH:cytochrome B5 reductase 1	[more]
AT5G20080.1	2.6e-54	40.29	FAD/NAD(P)-binding oxidoreductase	[more]
AT1G37130.1	1.0e-42	38.34	nitrate reductase 2	[more]
AT1G77760.1	3.7e-40	34.84	nitrate reductase 1	[more]

BLAST of Cucsa.022170 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449453115\|ref\|XP_004144304.1\|	2.8e-159	100.00	PREDICTED: NADH--cytochrome b5 reductase 1 [Cucumis sativus]	[more]
gi\|659111527\|ref\|XP_008455773.1\|	1.6e-157	98.58	PREDICTED: NADH--cytochrome b5 reductase 1 [Cucumis melo]	[more]
gi\|1012352800\|gb\|KYP63988.1\|	3.2e-134	83.27	NADH-cytochrome b5 reductase 1 [Cajanus cajan]	[more]
gi\|703080251\|ref\|XP_010091372.1\|	2.0e-133	81.49	NADH-cytochrome b5 reductase 1 [Morus notabilis]	[more]
gi\|567889474\|ref\|XP_006437259.1\|	2.0e-133	83.99	hypothetical protein CICLE_v10032349mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001433	OxRdtase_FAD/NAD-bd
IPR001834	NADH-Cyt_B5_reductase
IPR008333	OxRdtase_FAD-bd_dom
IPR017927	Fd_Rdtase_FAD-bd
IPR017938	Riboflavin_synthase-like_b-brl

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006040	amino sugar metabolic process
biological_process	GO:0042732	D-xylose metabolic process
biological_process	GO:0022900	electron transport chain
biological_process	GO:0006499	N-terminal protein myristoylation
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0009505	plant-type cell wall
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.022170.1	Cucsa.022170.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 158..264 score: 5.0
IPR001834	NADH:cytochrome b5 reductase (CBR)	PRINTS	PR00406	CYTB5RDTASE	coord: 157..176 score: 7.8E-30coord: 251..259 score: 7.8E-30coord: 134..148 score: 7.8E-30coord: 76..87 score: 7.8E-30coord: 98..105 score: 7.8E-30coord: 195..206 score: 7.8
IPR008333	Oxidoreductase, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 50..148 score: 4.6
IPR017927	Ferredoxin reductase-type FAD-binding domain	PROFILE	PS51384	FAD_FR	coord: 45..149 score: 13
IPR017938	Riboflavin synthase-like beta-barrel	unknown	SSF63380	Riboflavin synthase domain-like	coord: 42..150 score: 1.18
None	No IPR available	GENE3D	G3DSA:2.40.30.10		coord: 41..136 score: 1.6
None	No IPR available	GENE3D	G3DSA:3.40.50.80		coord: 137..279 score: 1.3
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 28..281 score: 8.3E
None	No IPR available	PANTHER	PTHR19370:SF111	SUBFAMILY NOT NAMED	coord: 28..281 score: 8.3E
None	No IPR available	unknown	SSF52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 137..278 score: 9.04

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.022170	Cla012157	Watermelon (97103) v1	cgywmB589
Cucsa.022170	Csa4G416480	Cucumber (Chinese Long) v2	cgycuB463
Cucsa.022170	MELO3C019165	Melon (DHL92) v3.5.1	cgymeB535
Cucsa.022170	ClCG04G005060	Watermelon (Charleston Gray)	cgywcgB560
Cucsa.022170	CSPI04G18660	Wild cucumber (PI 183967)	cgycpiB489
Cucsa.022170	Lsi01G017360	Bottle gourd (USVL1VR-Ls)	cgylsiB531
Cucsa.022170	MELO3C019165.2	Melon (DHL92) v3.6.1	cgymedB535
Cucsa.022170	CsaV3_4G029030	Cucumber (Chinese Long) v3	cgycucB504
Cucsa.022170	Cla97C04G071620	Watermelon (97103) v2	cgywmbB564
Cucsa.022170	Bhi03G002096	Wax gourd	cgywgoB657

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None