Cucsa.017500 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.017500 vs. TrEMBL
Match: A0A0A0LQV7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G433400 PE=4 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 9.7e-247
Identity = 444/484 (91.74%), Postives = 448/484 (92.56%), Query Frame = 1

Query: 1   LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKIVSVNPTKL 60
           LPTSMSELK+LKVLVVLDCSNLVVIHKN ISSMTKLEELDIQGRFTKWREKIVSVNPTKL
Sbjct: 61  LPTSMSELKELKVLVVLDCSNLVVIHKNFISSMTKLEELDIQGRFTKWREKIVSVNPTKL 120

Query: 61  SILLEGAEELVIVNDSKGFANNIFKAIGNGYPLLKYLKILENSETPHLRGNDFTSLEWLV 120
           SILLEGAEEL                         YLKILENSETPHLRGNDFTSLEWLV
Sbjct: 121 SILLEGAEEL-------------------------YLKILENSETPHLRGNDFTSLEWLV 180

Query: 121 LKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMM 180
           LKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMM
Sbjct: 181 LKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMM 240

Query: 181 EEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLED 240
           EEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLED
Sbjct: 241 EEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLED 300

Query: 241 LSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLL 300
           LSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLL
Sbjct: 301 LSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLL 360

Query: 301 EMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPNLKKVEVSRCPKL 360
           EMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPNLKKVEVSRCPKL
Sbjct: 361 EMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPNLKKVEVSRCPKL 420

Query: 361 RSIFPPSFTKYMKEIEELIVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKEVVDERFW 420
           RSIFPPSFTKYMKEIEELIVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKE VDERFW
Sbjct: 421 RSIFPPSFTKYMKEIEELIVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKEAVDERFW 480

Query: 421 VMSKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVSYSIEELTIRGCLQLVQVFGN 480
           VMSKF KLKRLELVGCE   DDDDDDDKMISLPMEMSEV YSIEELTIRGCLQL+ + G 
Sbjct: 481 VMSKFFKLKRLELVGCE---DDDDDDDKMISLPMEMSEVLYSIEELTIRGCLQLILIGGW 516

Query: 481 DSYI 485
           +S I
Sbjct: 541 NSSI 516

BLAST of Cucsa.017500 vs. TrEMBL
Match: A0A0A0LQV7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G433400 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 7.4e-21
Identity = 114/407 (28.01%), Postives = 180/407 (44.23%), Query Frame = 1

Query: 134 HSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMMEEIVSIEIEDQIT 193
           H     F+KL+ VEI+ CK+LR  F  +I   L +L  ++I+ C ++E I  IE      
Sbjct: 289 HKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLLEMIFEIE------ 348

Query: 194 ICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLEDLSIFRANNLEMLW 253
               P TS            C TK      +VP           L  L +    +L+ +W
Sbjct: 349 ---KPKTS------------CDTK-----VVVP-----------LRHLYLQVLPSLKYVW 408

Query: 254 HKNG---TSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLLEMIFEIEKPK 313
            K+G    +F  L+ VE+S C KLR +FP +    +  ++ L +      E IF +E   
Sbjct: 409 DKDGDDVVAFPNLKKVEVSRCPKLRSIFPPSFTKYMKEIEELIVED----EPIFPVEDEA 468

Query: 314 TSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDD----VVAFPNLKKVEVSRCP-------K 373
           +      +        Q L +LK    +  D+    +  F  LK++E+  C        K
Sbjct: 469 SKLKEVAL-------FQSLKTLKMSCKEAVDERFWVMSKFFKLKRLELVGCEDDDDDDDK 528

Query: 374 LRSIFPPSFTKYMKEIEELIVE-------------DEPIFPVED-----EASKLKEVALF 433
           + S+ P   ++ +  IEEL +                PI P +       A    E+   
Sbjct: 529 MISL-PMEMSEVLYSIEELTIRGCLQLILIGGWNSSIPILPTQGINDIIHARFTFEIGSL 588

Query: 434 QSLKTLKMSCKEVVDERFWVMSK-FSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVS 493
           Q ++ L++S K V  + FW  S+  SKL  L ++GCED        + ++ LP+EMSE+ 
Sbjct: 589 QGMRHLELSLKSV-KKSFWHKSESVSKLIGLTMIGCED--------EDIVCLPLEMSELL 637

Query: 494 YSIEELTIRGCLQLVQVF-------GNDSYIQRCANLKNMNQMTATT 501
           Y+IEE  I    +LVQVF        N++ +QRCA LKN+  +T ++
Sbjct: 649 YNIEEFIIEDAHKLVQVFENEELSRSNNNDVQRCAKLKNLTLITESS 637

HSP 2 Score: 54.7 bits (130), Expect = 4.8e-04
Identity = 66/250 (26.40%), Postives = 116/250 (46.40%), Query Frame = 1

Query: 371 YMKEIEEL-IVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKEVVDERFWVMSKFSKLK 430
           ++KE+E L I   E I  +    S+LKE+ +   L  L  S   V+ + F  +S  +KL+
Sbjct: 43  HLKELEILRICNCEGITELPTSMSELKELKV---LVVLDCSNLVVIHKNF--ISSMTKLE 102

Query: 431 RLELVGCEDDDDDDDDDDKMISL-PMEMSEVSYSIEELTIRGC--LQLVQVFGNDSYIQR 490
            L++ G           +K++S+ P ++S +    EEL ++     +   + GND     
Sbjct: 103 ELDIQG-----RFTKWREKIVSVNPTKLSILLEGAEELYLKILENSETPHLRGNDFTSLE 162

Query: 491 CANLKNMNQMTATT--------FSKLVDLQVHDCNGMINLFSPSVAKNLANLKSVEICNC 550
              LK M  + +          F+KL  +++  C  + N FS S+ K L+NL+ ++I  C
Sbjct: 163 WLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISEC 222

Query: 551 REMTSIVAAKAEEEEENVEIVFNNLTNMEFDNLERLEWFYSRKCRFE---FPILDTLRID 604
             M  IV+    E E+ + I  + LT++   ++ +L  F S K   +    P  D  R+ 
Sbjct: 223 DMMEEIVSI---EIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVS 279

HSP 3 Score: 814.7 bits (2103), Expect = 7.9e-233
Identity = 439/577 (76.08%), Postives = 479/577 (83.02%), Query Frame = 1

Query: 49   REKIVSVNPTKLSILLEGAEELVIVNDSKGFANNIFKAIGNGYPLLKYLKILENSETPHL 108
            + +IVSVN TKLSILLEG + L+I+NDSKGFAN+IFKAIGNGYPLLK L+I +NSETPHL
Sbjct: 743  KSQIVSVNGTKLSILLEGTKRLMILNDSKGFANDIFKAIGNGYPLLKCLEIHDNSETPHL 802

Query: 109  RGNDFTSLEWLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSN 168
            RGNDFTSL+ LVL  MVMLESI+PRHSP NPFNKLK ++I RC+QLRNFF LS+FKGLSN
Sbjct: 803  RGNDFTSLKRLVLDRMVMLESIIPRHSPINPFNKLKFIKIGRCEQLRNFFPLSVFKGLSN 862

Query: 169  LREIKISECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFF 228
            LR+I+I EC+MMEEIVSIEIED ITI TSPLTSL I  +NKL SFCSTKSSIQQTIVP F
Sbjct: 863  LRQIEIYECNMMEEIVSIEIEDHITIYTSPLTSLRIERVNKLTSFCSTKSSIQQTIVPLF 922

Query: 229  DERRVSFPQLEDLSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVS 288
            DERRVSFP+L+ LSI RANNLEMLWHKNG+SFSKLQT+EIS CK+LRCVFPSNI TSLV 
Sbjct: 923  DERRVSFPELKYLSIGRANNLEMLWHKNGSSFSKLQTIEISDCKELRCVFPSNIATSLVF 982

Query: 289  LDTLEINCCGLLEMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPN 348
            LDTL+I  C LLEMIFEIEK KTS DTK VVPLR+L L  L +LKYVWDKD DDVVAFPN
Sbjct: 983  LDTLKIYGCELLEMIFEIEKQKTSGDTK-VVPLRYLSLGFLKNLKYVWDKDVDDVVAFPN 1042

Query: 349  LKKVEVSRCPKLRSIFPPSFTKYMKEIEEL-IVE--DEPIFPVEDEASKLKEVALFQSLK 408
            LKKV+V RCPKL+ IFP SFTKYMKEIEEL +VE  +  IFPV DEASKLKEVALFQSL+
Sbjct: 1043 LKKVKVGRCPKLKIIFPASFTKYMKEIEELEMVEPFNYEIFPV-DEASKLKEVALFQSLE 1102

Query: 409  TLKMSCKEVVDERFWVMSKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVSYSIEE 468
            TL+MSCK+ V ERFWVMSKF KLK LEL GCE        D KMISLPMEM+EV YSIEE
Sbjct: 1103 TLRMSCKQAVKERFWVMSKFFKLKSLELFGCE--------DGKMISLPMEMNEVLYSIEE 1162

Query: 469  LTIRGCLQLVQVFGNDSYIQRCAN---------------LKNMNQMTATTFSKLVDLQVH 528
            LTIRGCLQLV V GND YIQRCAN               LKNMNQMTATTFSKLV LQV 
Sbjct: 1163 LTIRGCLQLVDVIGNDYYIQRCANLKKLKLYNLPKLMYVLKNMNQMTATTFSKLVYLQVG 1222

Query: 529  DCNGMINLFSPSVAKNLANLKSVEICNCREMTSIVAAKAEEEEENVEIVFNNLTNMEFDN 588
             CNGMINLFSPSVAKNLANL S+EI +C EM ++VAAKAEEEEENVEIVF+ LT MEF N
Sbjct: 1223 GCNGMINLFSPSVAKNLANLNSIEIYDCGEMRTVVAAKAEEEEENVEIVFSKLTGMEFHN 1282

Query: 589  LERLEWFYSRKCRFEFPILDTLRIDKCYDMKIFSYGI 608
            L  LE FY  KC  EFP+LDTLRI KC DMKIFSYGI
Sbjct: 1283 LAGLECFYPGKCTLEFPLLDTLRISKCDDMKIFSYGI 1309

BLAST of Cucsa.017500 vs. TrEMBL
Match: A0A0A0K5R3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G239020 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.5e-10
Identity = 39/52 (75.00%), Postives = 43/52 (82.69%), Query Frame = 1

Query: 1   LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKI 53
           LPTSMSELK+LKVLVV  C  LVVIH NIISSMTKLEELDIQ  F +W E++
Sbjct: 611 LPTSMSELKQLKVLVVSHCFKLVVIHTNIISSMTKLEELDIQDCFKEWGEEV 662

HSP 2 Score: 53.9 bits (128), Expect = 8.2e-04
Identity = 61/233 (26.18%), Postives = 103/233 (44.21%), Query Frame = 1

Query: 17   LDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKIVSVNPTKLSILLEGAEELVIVNDS 76
            + C   V     ++S   KL+ L++ G       K++S+ P +++ +L   EEL I    
Sbjct: 1104 MSCKQAVKERFWVMSKFFKLKSLELFGCEDG---KMISL-PMEMNEVLYSIEELTIRG-- 1163

Query: 77   KGFANNIFKAIGNGYPLLKY--LKILENSETPHLRGNDFTSLEWLVLKGMVMLESIVPRH 136
                  +   IGN Y + +   LK L+    P L           VLK M  + +     
Sbjct: 1164 ---CLQLVDVIGNDYYIQRCANLKKLKLYNLPKLM---------YVLKNMNQMTATT--- 1223

Query: 137  SPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMMEEIVSI---EIEDQ 196
                 F+KL  +++  C  + N FS S+ K L+NL  I+I +C  M  +V+    E E+ 
Sbjct: 1224 -----FSKLVYLQVGGCNGMINLFSPSVAKNLANLNSIEIYDCGEMRTVVAAKAEEEEEN 1283

Query: 197  ITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLEDLSIF 245
            + I  S LT +   ++  L  F   K +++    P  D  R+S  + +D+ IF
Sbjct: 1284 VEIVFSKLTGMEFHNLAGLECFYPGKCTLE---FPLLDTLRIS--KCDDMKIF 1305

HSP 3 Score: 224.2 bits (570), Expect = 4.6e-55
Identity = 154/396 (38.89%), Postives = 230/396 (58.08%), Query Frame = 1

Query: 60   LSILLEGAEELVIVNDSKG-FANNIFKAIGNGYPLLKYLKILE---NSETPHLRGNDFTS 119
            L ILL+ +E L IV+DSKG F N +FK  GNGYP LKYL +++   NSE  HL G+DFTS
Sbjct: 747  LEILLQRSERL-IVSDSKGNFINAMFKPNGNGYPCLKYLWMIDENGNSEMAHLIGSDFTS 806

Query: 120  LEWLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKIS 179
            L++L++ GM  LE+IVPRH   +PF K+K + I  C Q+RN FS SIFK L +L+EI++ 
Sbjct: 807  LKYLIIFGMKRLENIVPRHISLSPFKKVKTIAIQFCGQIRNLFSFSIFKDLLDLQEIEVI 866

Query: 180  ECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQ---QTIVPFFDERR 239
             C  ME I+ +EI DQ+ IC+ PLTSL + +++KL SFC TK  IQ   Q+I+PFFD  +
Sbjct: 867  NCGKMEGIIFMEIGDQLNICSCPLTSLQLENVDKLTSFC-TKDLIQESSQSIIPFFD-GQ 926

Query: 240  VSFPQLEDLSIFRANNLEMLWHKNG---TSFSKLQTVEISYCKKLRCVFPSNIVTSLV-- 299
            VSFP+L DLSI   NNLE LWHKN    T    L  + +     L+ V+  +I+  L   
Sbjct: 927  VSFPELNDLSIVGGNNLETLWHKNNNPTTVLWSLNELHLLNLPNLKQVWRKDIIKILTFP 986

Query: 300  SLDTLEINCCGLLEMIF-EIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAF 359
            SL  ++I+ C  L  ++ +  K   S D+     L  + ++   +LKY+        +AF
Sbjct: 987  SLKRVKIHGCTKLTHVWKDNNKVTRSFDS-----LERIEVEKCKNLKYLLPSS----IAF 1046

Query: 360  PNLKKVEVSRCPKLRSIFPPSFTKYMKEIEELIVE--DEPIFPVEDEASKLKEVALFQSL 419
             NLK++ + +C  + ++F  + TK +  +  + V         VE + ++  E+  F+ L
Sbjct: 1047 LNLKELHIKKCNGMINLFSSTVTKKLVNLSSIKVSYCKGMRCMVEVDQAENDEIITFKKL 1106

Query: 420  KTLKMSCKEVVDERF--WVMSKFSKLKRLELVGCED 439
             TL++     +D  +    M +F  L+ L +  C +
Sbjct: 1107 STLELDYLPRLDSFYSGKCMLEFPCLESLVIKRCPE 1130

BLAST of Cucsa.017500 vs. TrEMBL
Match: A0A0A0LTN4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G433370 PE=3 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.9e-25
Identity = 110/391 (28.13%), Postives = 180/391 (46.04%), Query Frame = 1

Query: 235  FPQLEDLSIFRANNLEMLW--HKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTL 294
            F  L+ L IF    LE +   H + + F K++T+ I +C ++R +F  +I   L+ L  +
Sbjct: 803  FTSLKYLIIFGMKRLENIVPRHISLSPFKKVKTIAIQFCGQIRNLFSFSIFKDLLDLQEI 862

Query: 295  EINCCGLLE--MIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKD----------- 354
            E+  CG +E  +  EI      C      PL  L L+ +  L     KD           
Sbjct: 863  EVINCGKMEGIIFMEIGDQLNICS----CPLTSLQLENVDKLTSFCTKDLIQESSQSIIP 922

Query: 355  -GDDVVAFPNLKKVEVSRCPKLRSIFPPSF--TKYMKEIEELIVEDEPIFPVEDEASKLK 414
              D  V+FP L  + +     L +++  +   T  +  + EL + + P          LK
Sbjct: 923  FFDGQVSFPELNDLSIVGGNNLETLWHKNNNPTTVLWSLNELHLLNLP---------NLK 982

Query: 415  EVALFQSLKTLKMSCKEVVDERFWVMSKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEM 474
            +V     +K L                 F  LKR+++ GC           K+  +  + 
Sbjct: 983  QVWRKDIIKIL----------------TFPSLKRVKIHGCT----------KLTHVWKDN 1042

Query: 475  SEVSYSIEELTIRGCLQLVQVFGNDSYIQRCANLKNMNQMTATTFSKLVDLQVHDCNGMI 534
            ++V+ S +       L+ ++V       ++C NLK +   ++  F  L +L +  CNGMI
Sbjct: 1043 NKVTRSFDS------LERIEV-------EKCKNLKYL-LPSSIAFLNLKELHIKKCNGMI 1102

Query: 535  NLFSPSVAKNLANLKSVEICNCREMTSIVAAKAEEEEENVEIVFNNLTNMEFDNLERLEW 594
            NLFS +V K L NL S+++  C+ M  +V  + ++ E +  I F  L+ +E D L RL+ 
Sbjct: 1103 NLFSSTVTKKLVNLSSIKVSYCKGMRCMV--EVDQAENDEIITFKKLSTLELDYLPRLDS 1138

Query: 595  FYSRKCRFEFPILDTLRIDKCYDMKIFSYGI 608
            FYS KC  EFP L++L I +C +MK FSYG+
Sbjct: 1163 FYSGKCMLEFPCLESLVIKRCPEMKTFSYGV 1138

HSP 2 Score: 59.3 bits (142), Expect = 2.0e-05
Identity = 29/52 (55.77%), Postives = 37/52 (71.15%), Query Frame = 1

Query: 1   LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKI 53
           LP +MS+L  LKVL VL+C  L V+  NI SSMTKLEEL +Q  F +W E++
Sbjct: 610 LPPTMSQLTHLKVLEVLNCPKLEVVPANIFSSMTKLEELKLQDSFCRWGEEV 661

HSP 3 Score: 53.5 bits (127), Expect = 1.1e-03
Identity = 59/232 (25.43%), Postives = 106/232 (45.69%), Query Frame = 1

Query: 32   SMTKLEELDIQGRF---TKWREKIVSVNPTKLSILLEGAEELVIVNDSKGFANNIFKAIG 91
            S  +L +L I G     T W +   + NPT +   L     L + N  + +  +I K + 
Sbjct: 925  SFPELNDLSIVGGNNLETLWHK---NNNPTTVLWSLNELHLLNLPNLKQVWRKDIIKILT 984

Query: 92   NGYPLLKYLKILENSETPHLRGND------FTSLEWLVLKGMVMLESIVPRHSPTNPFNK 151
              +P LK +KI   ++  H+  ++      F SLE + ++    L+ ++P    +  F  
Sbjct: 985  --FPSLKRVKIHGCTKLTHVWKDNNKVTRSFDSLERIEVEKCKNLKYLLPS---SIAFLN 1044

Query: 152  LKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMMEEIVSI-EIEDQITICTSPLTS 211
            LK + I +C  + N FS ++ K L NL  IK+S C  M  +V + + E+   I    L++
Sbjct: 1045 LKELHIKKCNGMINLFSSTVTKKLVNLSSIKVSYCKGMRCMVEVDQAENDEIITFKKLST 1104

Query: 212  LHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLEDLSIFRANNLEMLW 254
            L + ++ +L SF S K  ++   +     +R    +     +  A  L+ LW
Sbjct: 1105 LELDYLPRLDSFYSGKCMLEFPCLESLVIKRCPEMKTFSYGVIIAPRLQTLW 1148

HSP 4 Score: 183.3 bits (464), Expect = 9.0e-43
Identity = 163/541 (30.13%), Postives = 267/541 (49.35%), Query Frame = 1

Query: 89   NGYPLLKYLKILENSETPHL---RGNDFTS----LEWLVLKGMVMLESIVPRHSPTNPFN 148
            N +  LKYL I +N E  H    + N        LE+L L+ +  L++I+  +   + F+
Sbjct: 782  NEFLHLKYLYISDNLEFQHFIHEKNNPLRKCLPKLEYLYLEELENLKNIIHGYHRESLFS 841

Query: 149  KLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMMEEIVSIEIEDQIT-ICTSPLT 208
            KLK V +T+C +L   F   I   + +L EI I  C+ ME ++ +E E+    I  + L 
Sbjct: 842  KLKSVVVTKCNKLEKLFFNCILDDILSLEEIAIHYCEKMEVMIVMENEEATNHIEFTHLK 901

Query: 209  SLHICHMNKLASFC---------STKSSIQQTI---VPFFDERRVSFPQLEDLSIFRANN 268
             L + ++ +L  FC         S  +SI  T+     FF+E  VS P LE L I  A N
Sbjct: 902  YLFLTYVPQLQKFCSKIEKFGQLSQDNSISNTVDIGESFFNE-EVSLPNLEKLGIKCAEN 961

Query: 269  LEMLWHKN---GTSFSKLQTVEISYCKKL-RCVFPSNIVTSLVSLDTLEINCCGLLEMIF 328
            L M+W  N     SFSKL+ VEI+ C  L + +FPSN+++ L  L  L INCC LLE IF
Sbjct: 962  LTMIWCNNVHFPNSFSKLEEVEIASCNNLHKVLFPSNVMSILTCLKVLRINCCKLLEGIF 1021

Query: 329  EIEKPKTSCDTKVVV--PLRHLYLQVLPSLKYVWDKDGDDVVAFPNLKKVEVSRCPKLRS 388
            E+++   + DT ++V   LR L L  LP+L+YVW K+  ++++F N+K + +  CP+LR 
Sbjct: 1022 EVQESSIT-DTSLIVLKNLRELKLYNLPNLEYVWSKNPCELLSFVNIKGLAIDECPRLRR 1081

Query: 389  IFPPSFTKYMKEIEELIVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKEVVDERFWVM 448
             +     K +K++E L ++ + +  V  E  K  +  + +S K L+ S K  V       
Sbjct: 1082 EYS---VKILKQLERLTMDIKQLMEV-IENQKSTDHNMVKS-KQLETSSKVEVLLTGDGS 1141

Query: 449  SKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVSYSIEELTIRGCLQLVQVFGNDS 508
              F  LK L L G  +D+           LP+E+ ++ Y +E   + G   + +VF ++ 
Sbjct: 1142 ELFPNLKELTLYGFVEDNS--------THLPVEIVQILYQLEHFELEGA-YIEEVFPSNI 1201

Query: 509  YI--QRCANLKNMNQMTATTFSKLVDLQVHDCNGMINLFSPSVAKN----LANLKSVEIC 568
             I  ++    ++ N + +   SKL  L+        +L+S    KN    L +L  + I 
Sbjct: 1202 LIPMKKQYYARSKNSVRSWFLSKLPKLR--------HLWSECSQKNAFPILQDLNVIRIS 1261

Query: 569  NCREMTSIVAAKAEEEEENVEIVFNNLTNMEFDNLERLEWFYSRKCRFEFPILDTLRIDK 598
             C  ++S+V++          + F NLT ++ D  +RL +  +         L+ L + +
Sbjct: 1262 ECGGLSSLVSS---------SVSFTNLTVLKVDKCDRLTYLLNPLVATTLVQLEELTLRE 1289

BLAST of Cucsa.017500 vs. TrEMBL
Match: A0A0A0LLJ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G014830 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.5e-29
Identity = 133/498 (26.71%), Postives = 227/498 (45.58%), Query Frame = 1

Query: 140  FNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMMEEIV---------SIEIED 199
            F  L V+++ +C +L    +  +   L  L E+ + EC MM  ++         + E  +
Sbjct: 1252 FTNLTVLKVDKCDRLTYLLNPLVATTLVQLEELTLRECKMMSSVIEGGSAEEDGNEETTN 1311

Query: 200  QITICTSPLTSLHICHMNKLASFCST----------------KSSIQQTIVPFFDERRVS 259
            QI    + L SL +  + +L  F S                  ++I   I   F   + S
Sbjct: 1312 QIEF--THLKSLFLKDLPRLQKFYSKIETFGQLSRDNSENPETTTIHNRIGDSFFSEQES 1371

Query: 260  FPQLEDLSIFRANNLEMLWHKN---GTSFSKLQTVEISYCKKLRCV-FPSNIVTSLVSLD 319
             P LE L I  A NL M+W  N     SFSKL+ VEI  C  L+ V F  NI+  L  L+
Sbjct: 1372 LPNLETLRIDGAENLRMIWSNNVLIPNSFSKLEEVEIYSCNNLQDVLFHPNIINMLTCLN 1431

Query: 320  TLEINCCGLLEMIFEIEKPKTSCDTK---VVVP--LRHLYLQVLPSLKYVWDKDG--DDV 379
            TL I  C LLE IFE+++P +   TK   +V+P  L  L L  LP+L+Y+W K+   + +
Sbjct: 1432 TLRIKNCELLEGIFEVQEPISVTKTKTNAIVLPNNLIELELYNLPNLEYLWSKNPNFERL 1491

Query: 380  VAFPNLKKVEVSRCPKLRSIFPPSFTKYMKEI--------------EELIVEDEPIF--- 439
            V F +++ + + +C KL+  +  S   + + +              +E+   D  +    
Sbjct: 1492 VTFESIRSLSIEKCSKLKGEYFLSIKTFKQLVRLKMGIRQLTVALGKEVKSADHSMLLEP 1551

Query: 440  -PVEDEASKLKEVAL------FQSLKTLKMSCKEVVDERFW---VMSKFSKLKRLELVGC 499
              +E  +SK++ + L      F +LK LK+      +       ++   ++L++ EL G 
Sbjct: 1552 KQLETSSSKVEVLQLRDGSKLFSNLKELKLYGFVEYNSTHLPMEIVQVLNQLEKFELKGM 1611

Query: 500  EDDDDDDDDDDKMISLPMEMSEVSYSI-EELTIRGCLQLVQVFG------NDS------- 559
              ++            P  +   SY +  ELT+    +L  ++G      NDS       
Sbjct: 1612 FIEE----------IFPSNILIPSYMVLRELTLSKLSKLRHLWGECSQKNNDSLLRDLTF 1671

HSP 2 Score: 75.9 bits (185), Expect = 2.0e-10
Identity = 96/423 (22.70%), Postives = 179/423 (42.32%), Query Frame = 1

Query: 167  SNLREIKISECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVP 226
            S L E++I+ C+ + +++       I  C   L  ++ C + +   F   +SSI  T + 
Sbjct: 976  SKLEEVEIASCNNLHKVLFPSNVMSILTCLKVLR-INCCKLLE-GIFEVQESSITDTSL- 1035

Query: 227  FFDERRVSFPQLEDLSIFRANNLEMLWHKNGT---SFSKLQTVEISYCKKLRCVFPSNIV 286
                  +    L +L ++   NLE +W KN     SF  ++ + I  C +LR  +   I+
Sbjct: 1036 ------IVLKNLRELKLYNLPNLEYVWSKNPCELLSFVNIKGLAIDECPRLRREYSVKIL 1095

Query: 287  TSLVSLDTLEINCCGLLEMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDV 346
              L  L T++I      + + E+ + + S D  +V   +   L+    ++ +   DG ++
Sbjct: 1096 KQLERL-TMDI------KQLMEVIENQKSTDHNMV---KSKQLETSSKVEVLLTGDGSEL 1155

Query: 347  VAFPNLKKVEVSRCPKLRSIFPPSFTKYMKEIEELIVEDEPIFPVEDEASKLKEVALFQS 406
              FPNLK++       L      + T    EI +++ + E     E E + ++EV     
Sbjct: 1156 --FPNLKELT------LYGFVEDNSTHLPVEIVQILYQLEHF---ELEGAYIEEVFPSNI 1215

Query: 407  LKTLKMS--CKEVVDERFWVMSKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVSY 466
            L  +K     +     R W +SK  KL+ L                              
Sbjct: 1216 LIPMKKQYYARSKNSVRSWFLSKLPKLRHLW----------------------------- 1275

Query: 467  SIEELTIRGCLQLVQVFGNDSYIQRCANLKNMNQMTATTFSKLVDLQVHDCNGMINLFSP 526
               E + +    ++Q   N   I  C  L ++   ++ +F+ L  L+V  C+ +  L +P
Sbjct: 1276 --SECSQKNAFPILQDL-NVIRISECGGLSSLVS-SSVSFTNLTVLKVDKCDRLTYLLNP 1335

Query: 527  SVAKNLANLKSVEICNCREMTSIVAAKAEEEEENVE----IVFNNLTNMEFDNLERLEWF 581
             VA  L  L+ + +  C+ M+S++   + EE+ N E    I F +L ++   +L RL+ F
Sbjct: 1336 LVATTLVQLEELTLRECKMMSSVIEGGSAEEDGNEETTNQIEFTHLKSLFLKDLPRLQKF 1335

HSP 3 Score: 68.6 bits (166), Expect = 3.2e-08
Identity = 103/482 (21.37%), Postives = 185/482 (38.38%), Query Frame = 1

Query: 168  NLREIKISECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPF 227
            N++ + I EC  +    S++I  Q+   T  +  L    M  + +  ST           
Sbjct: 1065 NIKGLAIDECPRLRREYSVKILKQLERLTMDIKQL----MEVIENQKST----------- 1124

Query: 228  FDERRVSFPQLEDLSIFRANNLEMLWHKNGTS-FSKLQTVEI-SYCKKLRCVFPSNIVTS 287
             D   V   QLE  S      +E+L   +G+  F  L+ + +  + +      P  IV  
Sbjct: 1125 -DHNMVKSKQLETSS-----KVEVLLTGDGSELFPNLKELTLYGFVEDNSTHLPVEIVQI 1184

Query: 288  LVSLDTLEINCCGLLEMIFE---IEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGD- 347
            L  L+  E+     +E +F    +   K     +    +R  +L  LP L+++W +    
Sbjct: 1185 LYQLEHFELEGA-YIEEVFPSNILIPMKKQYYARSKNSVRSWFLSKLPKLRHLWSECSQK 1244

Query: 348  -------------------------DVVAFPNLKKVEVSRCPKLRSIFPPSFTKYMKEIE 407
                                       V+F NL  ++V +C +L  +  P     + ++E
Sbjct: 1245 NAFPILQDLNVIRISECGGLSSLVSSSVSFTNLTVLKVDKCDRLTYLLNPLVATTLVQLE 1304

Query: 408  ELIVED--------EPIFPVEDEASKLKEVALFQSLKTLKMSCKEVVDERFWVMSKFSKL 467
            EL + +        E     ED   +      F  LK+L +     + + +  +  F +L
Sbjct: 1305 ELTLRECKMMSSVIEGGSAEEDGNEETTNQIEFTHLKSLFLKDLPRLQKFYSKIETFGQL 1364

Query: 468  KRLELVGCEDDDDDDDDDDKMISLPMEMSEVSYSIEELTIRGCLQLVQVFGNDSYIQRCA 527
             R      E     +   D   S      E   ++E L I G   L  ++ N        
Sbjct: 1365 SRDNSENPETTTIHNRIGDSFFS----EQESLPNLETLRIDGAENLRMIWSN-------- 1424

Query: 528  NLKNMNQMTATTFSKLVDLQVHDCNGMIN-LFSPSVAKNLANLKSVEICNCREMTSIVAA 587
                 N +   +FSKL +++++ CN + + LF P++   L  L ++ I NC  +  I   
Sbjct: 1425 -----NVLIPNSFSKLEEVEIYSCNNLQDVLFHPNIINMLTCLNTLRIKNCELLEGIFEV 1484

Query: 588  K----AEEEEENVEIVFNNLTNMEFDNLERLEWFYSRKCRFE----FPILDTLRIDKCYD 602
            +      + + N  ++ NNL  +E  NL  LE+ +S+   FE    F  + +L I+KC  
Sbjct: 1485 QEPISVTKTKTNAIVLPNNLIELELYNLPNLEYLWSKNPNFERLVTFESIRSLSIEKCSK 1507

HSP 4 Score: 47.4 bits (111), Expect = 7.7e-02
Identity = 76/344 (22.09%), Postives = 139/344 (40.41%), Query Frame = 1

Query: 1   LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKIVSVNPTKL 60
           +P + S+L +LKVL +  C  L VI  NI+S +TK                         
Sbjct: 626 IPMTFSKLTQLKVLNLSFCDELEVIPPNILSKLTK------------------------- 685

Query: 61  SILLEGAEELVIVNDSKGFANNIFKAIGNG-YPLLKYLKILENSETPHLRGNDFTSLEWL 120
                  EEL +           ++   N     L+YL        PHL   + T     
Sbjct: 686 ------LEELHLETFDSWEGEEWYEGRKNASLSELRYL--------PHLYALNLT----- 745

Query: 121 VLKGMVMLESIVPRHSPTNPFNKLKVVEIT-RCKQLRNFFSLSIFKGLSNLREIKISECD 180
                +  + I+P+H        L+   IT  C++ +           +N   IK+    
Sbjct: 746 -----IQDDEIMPKHLFLAGELNLENFHITIGCQRQKRHID-----NKTNFFRIKMESER 805

Query: 181 MMEEIV------SIEIEDQITICTSPLTSLH-ICHMNKLASFCSTKSSIQQTIVPFFDER 240
            +++ +      S E+  + +IC+  L   +   H+  L  + S     Q  I    +  
Sbjct: 806 CLDDWIKTLLKRSEEVHLKGSICSKVLHDANEFLHLKYL--YISDNLEFQHFIHEKNNPL 865

Query: 241 RVSFPQLEDLSIFRANNLEMLWH--KNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSL 300
           R   P+LE L +    NL+ + H     + FSKL++V ++ C KL  +F + I+  ++SL
Sbjct: 866 RKCLPKLEYLYLEELENLKNIIHGYHRESLFSKLKSVVVTKCNKLEKLFFNCILDDILSL 912

Query: 301 DTLEINCCGLLEMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLK 334
           + + I+ C  +E++  +E  + +   +    L++L+L  +P L+
Sbjct: 926 EEIAIHYCEKMEVMIVMENEEATNHIE-FTHLKYLFLTYVPQLQ 912

HSP 5 Score: 174.1 bits (440), Expect = 5.4e-40
Identity = 169/673 (25.11%), Postives = 306/673 (45.47%), Query Frame = 1

Query: 4    SMSELKKLKVLVVLDCS--NLVVIHKNIISSMTKL------EELDIQGRFTKWREKIVSV 63
            S+ ELK L  LV L+    N  ++ +++ S    L      EE    G++   R   + +
Sbjct: 619  SLDELKLLPNLVTLELHIINAEILPRDVFSEKLDLYKVFIGEEWSWFGKYEASRTLKLKL 678

Query: 64   NPT----KLSILLEGAEELVIVNDSKGFANNIFKAIGNGYPLLKYLKILENSETPHL--- 123
            N +    K+ +LL   E+L + ++ +G  N +++  G G+P LK+L I  +SE  ++   
Sbjct: 679  NSSIEIEKVKVLLMTTEDLYL-DELEGVRNVLYELDGQGFPQLKHLHIQNSSEIQYIVDC 738

Query: 124  --RGND---FTSLEWLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIF 183
               GN    F  LE L++  +  L  I      +  F+KL+ +++  C  L+N F  S+F
Sbjct: 739  LSMGNHYIAFPRLESLLVDNLNNLGQICYGQLMSGSFSKLRKLKVEHCNALKNLFYFSMF 798

Query: 184  KGLSNLREIKISECDMMEEIVSIEIEDQI----TICTSPLTSLHICHMNKLASFCSTKSS 243
            +GL  L EI +S C++MEEIV  EIED       I    L +L + ++ +  SFCS +  
Sbjct: 799  RGLVQLEEIDVSSCNIMEEIVVEEIEDDSGRDEIIKPIRLRTLTLEYLPRFTSFCSQRMQ 858

Query: 244  ------------IQQTIVPFFDERRVSFPQLEDLSIFRANNLEMLWHKN----GTSFSKL 303
                        I +T    F + ++ F  L +L +   NN+E +W        +S   L
Sbjct: 859  KLAGLDAGCAQIISETPSVLFGQ-KIEFSNLLNLKLSSINNMEKIWRNQVKEPPSSVQNL 918

Query: 304  QTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLLEMIFEIEKPKTSCDTKVVVPLRH 363
             ++ +  C KL  +F S++V +L  L+ LEI+ C  +E I  + +  T  ++K+  P+ H
Sbjct: 919  TSLIVEGCGKLSYLFTSSMVENLSQLEYLEISDCSFMEEII-VAEGLTKHNSKLHFPILH 978

Query: 364  -LYLQVLPSL-KYVWDKDGDDVVAFPNLKKVEVSRCPKLRSIFPPSFTKYM------KEI 423
             L L+ LP+L ++ +     +++  P+L  + +  CP+L      S +  M      +E 
Sbjct: 979  TLKLKSLPNLIRFCF----GNLIECPSLNALRIENCPRLLKFISSSASTNMEANRGGRET 1038

Query: 424  EELIVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKEVVDERFWVMSKFSKLKRLELVG 483
               + +++  FP+             + L+ + M+   ++ E       F KLK +++  
Sbjct: 1039 NSTLFDEKVSFPI------------LEKLEIVYMNNLRMIWESEDRGDSFCKLKIVKIQN 1098

Query: 484  CEDDDDDDDDDDKMISLPMEMSEVSYSIEELTIRGCLQLVQVFG---------------- 543
            C+         + +   P +M      +E++ +  C  L +VF                 
Sbjct: 1099 CK---------ELVTIFPSKMLRALQKLEDVVVTNCDLLEEVFNLQELMATEGKQNRVLP 1158

Query: 544  -----NDSYIQRCANLKNM---NQMTATTFSKLVDLQVHDCNGMINLFSPSVAKNLANLK 603
                  D  I+   +LK++   +     +F  L  L   +C  + NLF  S+AK+L+ L+
Sbjct: 1159 VVAQLRDLTIENLPSLKHVWSGDPQGVFSFDNLRSLSAENCPSLKNLFPASIAKSLSQLE 1218

Query: 604  SVEICNCREMTSIVAAKAEEEEENVEIVFNNLTNMEFDNLERLEWFYSRKCRFEFPILDT 605
             + I NC  +  IVA   +  E     VF  L +M+   LE ++ FY  +   + P L+ 
Sbjct: 1219 DLSIVNC-GLQEIVA--KDRVEATPRFVFPQLKSMKLWILEEVKNFYPGRHILDCPKLEK 1260

BLAST of Cucsa.017500 vs. TAIR10
Match: AT1G69550.1 (AT1G69550.1 disease resistance protein (TIR-NBS-LRR class))

HSP 1 Score: 52.0 bits (123), Expect = 1.6e-06
Identity = 101/394 (25.63%), Postives = 177/394 (44.92%), Query Frame = 1

Query: 1    LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKIVSVNPTKL 60
            +P+S+  L  LK+L +  CS+LV +  + I ++  L++LD+ G  +     +   N   L
Sbjct: 852  IPSSIGNLINLKLLNLSGCSSLVELPSS-IGNLINLKKLDLSGCSSLVELPLSIGNLINL 911

Query: 61   -SILLEGAEELVIVNDSKGFANNIFKAIGNGYPLLKYLKILENS---ETPHLRGNDFTSL 120
              + L     LV +  S G   N           LK L + E S   E P   GN   +L
Sbjct: 912  QELYLSECSSLVELPSSIGNLIN-----------LKTLNLSECSSLVELPSSIGN-LINL 971

Query: 121  EWLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISE 180
            + L L       S+V   S       LK ++++ C  L     LSI   L NL+ + +SE
Sbjct: 972  QELYLS---ECSSLVELPSSIGNLINLKKLDLSGCSSLVE-LPLSI-GNLINLKTLNLSE 1031

Query: 181  CDMMEEIVS-----IEIED-QITICTS----PLTSLHICHMNKL-ASFCSTKSSIQQTIV 240
            C  + E+ S     I +++  ++ C+S    P +  ++ ++ KL  S CS+   +  +I 
Sbjct: 1032 CSSLVELPSSIGNLINLQELYLSECSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIG 1091

Query: 241  PFFDERRVSFPQLEDLSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTS 300
               +        L+ L++   ++L  L    G     L+ +++S C  L    PS+I  +
Sbjct: 1092 NLIN--------LKTLNLSGCSSLVELPSSIGN--LNLKKLDLSGCSSL-VELPSSI-GN 1151

Query: 301  LVSLDTLEIN-CCGLLEMIFEI-------EKPKTSCDTKVVVP--------LRHLYLQVL 360
            L++L  L+++ C  L+E+   I       E   + C + V +P        L+ LYL   
Sbjct: 1152 LINLKKLDLSGCSSLVELPLSIGNLINLQELYLSECSSLVELPSSIGNLINLQELYLSEC 1211

Query: 361  PSLKYVWDKDGDDVVAFPNLKKVEVSRCPKLRSI 364
             SL  +    G+ +    NLKK+++++C KL S+
Sbjct: 1212 SSLVELPSSIGNLI----NLKKLDLNKCTKLVSL 1211

BLAST of Cucsa.017500 vs. NCBI nr
Match: gi|700208255|gb|KGN63374.1| (hypothetical protein Csa_2G433400 [Cucumis sativus])

HSP 1 Score: 860.9 bits (2223), Expect = 1.4e-246
Identity = 444/484 (91.74%), Postives = 448/484 (92.56%), Query Frame = 1

Query: 1   LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKIVSVNPTKL 60
           LPTSMSELK+LKVLVVLDCSNLVVIHKN ISSMTKLEELDIQGRFTKWREKIVSVNPTKL
Sbjct: 61  LPTSMSELKELKVLVVLDCSNLVVIHKNFISSMTKLEELDIQGRFTKWREKIVSVNPTKL 120

Query: 61  SILLEGAEELVIVNDSKGFANNIFKAIGNGYPLLKYLKILENSETPHLRGNDFTSLEWLV 120
           SILLEGAEEL                         YLKILENSETPHLRGNDFTSLEWLV
Sbjct: 121 SILLEGAEEL-------------------------YLKILENSETPHLRGNDFTSLEWLV 180

Query: 121 LKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMM 180
           LKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMM
Sbjct: 181 LKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMM 240

Query: 181 EEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLED 240
           EEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLED
Sbjct: 241 EEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLED 300

Query: 241 LSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLL 300
           LSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLL
Sbjct: 301 LSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLL 360

Query: 301 EMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPNLKKVEVSRCPKL 360
           EMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPNLKKVEVSRCPKL
Sbjct: 361 EMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPNLKKVEVSRCPKL 420

Query: 361 RSIFPPSFTKYMKEIEELIVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKEVVDERFW 420
           RSIFPPSFTKYMKEIEELIVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKE VDERFW
Sbjct: 421 RSIFPPSFTKYMKEIEELIVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKEAVDERFW 480

Query: 421 VMSKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVSYSIEELTIRGCLQLVQVFGN 480
           VMSKF KLKRLELVGCE   DDDDDDDKMISLPMEMSEV YSIEELTIRGCLQL+ + G 
Sbjct: 481 VMSKFFKLKRLELVGCE---DDDDDDDKMISLPMEMSEVLYSIEELTIRGCLQLILIGGW 516

Query: 481 DSYI 485
           +S I
Sbjct: 541 NSSI 516

BLAST of Cucsa.017500 vs. NCBI nr
Match: gi|700208255|gb|KGN63374.1| (hypothetical protein Csa_2G433400 [Cucumis sativus])

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-20
Identity = 114/407 (28.01%), Postives = 180/407 (44.23%), Query Frame = 1

Query: 134 HSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMMEEIVSIEIEDQIT 193
           H     F+KL+ VEI+ CK+LR  F  +I   L +L  ++I+ C ++E I  IE      
Sbjct: 289 HKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLLEMIFEIE------ 348

Query: 194 ICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLEDLSIFRANNLEMLW 253
               P TS            C TK      +VP           L  L +    +L+ +W
Sbjct: 349 ---KPKTS------------CDTK-----VVVP-----------LRHLYLQVLPSLKYVW 408

Query: 254 HKNG---TSFSKLQTVEISYCKKLRCVFPSNIVTSLVSLDTLEINCCGLLEMIFEIEKPK 313
            K+G    +F  L+ VE+S C KLR +FP +    +  ++ L +      E IF +E   
Sbjct: 409 DKDGDDVVAFPNLKKVEVSRCPKLRSIFPPSFTKYMKEIEELIVED----EPIFPVEDEA 468

Query: 314 TSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDD----VVAFPNLKKVEVSRCP-------K 373
           +      +        Q L +LK    +  D+    +  F  LK++E+  C        K
Sbjct: 469 SKLKEVAL-------FQSLKTLKMSCKEAVDERFWVMSKFFKLKRLELVGCEDDDDDDDK 528

Query: 374 LRSIFPPSFTKYMKEIEELIVE-------------DEPIFPVED-----EASKLKEVALF 433
           + S+ P   ++ +  IEEL +                PI P +       A    E+   
Sbjct: 529 MISL-PMEMSEVLYSIEELTIRGCLQLILIGGWNSSIPILPTQGINDIIHARFTFEIGSL 588

Query: 434 QSLKTLKMSCKEVVDERFWVMSK-FSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVS 493
           Q ++ L++S K V  + FW  S+  SKL  L ++GCED        + ++ LP+EMSE+ 
Sbjct: 589 QGMRHLELSLKSV-KKSFWHKSESVSKLIGLTMIGCED--------EDIVCLPLEMSELL 637

Query: 494 YSIEELTIRGCLQLVQVF-------GNDSYIQRCANLKNMNQMTATT 501
           Y+IEE  I    +LVQVF        N++ +QRCA LKN+  +T ++
Sbjct: 649 YNIEEFIIEDAHKLVQVFENEELSRSNNNDVQRCAKLKNLTLITESS 637

HSP 2 Score: 54.7 bits (130), Expect = 6.9e-04
Identity = 66/250 (26.40%), Postives = 116/250 (46.40%), Query Frame = 1

Query: 371 YMKEIEEL-IVEDEPIFPVEDEASKLKEVALFQSLKTLKMSCKEVVDERFWVMSKFSKLK 430
           ++KE+E L I   E I  +    S+LKE+ +   L  L  S   V+ + F  +S  +KL+
Sbjct: 43  HLKELEILRICNCEGITELPTSMSELKELKV---LVVLDCSNLVVIHKNF--ISSMTKLE 102

Query: 431 RLELVGCEDDDDDDDDDDKMISL-PMEMSEVSYSIEELTIRGC--LQLVQVFGNDSYIQR 490
            L++ G           +K++S+ P ++S +    EEL ++     +   + GND     
Sbjct: 103 ELDIQG-----RFTKWREKIVSVNPTKLSILLEGAEELYLKILENSETPHLRGNDFTSLE 162

Query: 491 CANLKNMNQMTATT--------FSKLVDLQVHDCNGMINLFSPSVAKNLANLKSVEICNC 550
              LK M  + +          F+KL  +++  C  + N FS S+ K L+NL+ ++I  C
Sbjct: 163 WLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISEC 222

Query: 551 REMTSIVAAKAEEEEENVEIVFNNLTNMEFDNLERLEWFYSRKCRFE---FPILDTLRID 604
             M  IV+    E E+ + I  + LT++   ++ +L  F S K   +    P  D  R+ 
Sbjct: 223 DMMEEIVSI---EIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVS 279

HSP 3 Score: 814.7 bits (2103), Expect = 1.1e-232
Identity = 439/577 (76.08%), Postives = 479/577 (83.02%), Query Frame = 1

Query: 49   REKIVSVNPTKLSILLEGAEELVIVNDSKGFANNIFKAIGNGYPLLKYLKILENSETPHL 108
            + +IVSVN TKLSILLEG + L+I+NDSKGFAN+IFKAIGNGYPLLK L+I +NSETPHL
Sbjct: 743  KSQIVSVNGTKLSILLEGTKRLMILNDSKGFANDIFKAIGNGYPLLKCLEIHDNSETPHL 802

Query: 109  RGNDFTSLEWLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSN 168
            RGNDFTSL+ LVL  MVMLESI+PRHSP NPFNKLK ++I RC+QLRNFF LS+FKGLSN
Sbjct: 803  RGNDFTSLKRLVLDRMVMLESIIPRHSPINPFNKLKFIKIGRCEQLRNFFPLSVFKGLSN 862

Query: 169  LREIKISECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFF 228
            LR+I+I EC+MMEEIVSIEIED ITI TSPLTSL I  +NKL SFCSTKSSIQQTIVP F
Sbjct: 863  LRQIEIYECNMMEEIVSIEIEDHITIYTSPLTSLRIERVNKLTSFCSTKSSIQQTIVPLF 922

Query: 229  DERRVSFPQLEDLSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVS 288
            DERRVSFP+L+ LSI RANNLEMLWHKNG+SFSKLQT+EIS CK+LRCVFPSNI TSLV 
Sbjct: 923  DERRVSFPELKYLSIGRANNLEMLWHKNGSSFSKLQTIEISDCKELRCVFPSNIATSLVF 982

Query: 289  LDTLEINCCGLLEMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPN 348
            LDTL+I  C LLEMIFEIEK KTS DTK VVPLR+L L  L +LKYVWDKD DDVVAFPN
Sbjct: 983  LDTLKIYGCELLEMIFEIEKQKTSGDTK-VVPLRYLSLGFLKNLKYVWDKDVDDVVAFPN 1042

Query: 349  LKKVEVSRCPKLRSIFPPSFTKYMKEIEEL-IVE--DEPIFPVEDEASKLKEVALFQSLK 408
            LKKV+V RCPKL+ IFP SFTKYMKEIEEL +VE  +  IFPV DEASKLKEVALFQSL+
Sbjct: 1043 LKKVKVGRCPKLKIIFPASFTKYMKEIEELEMVEPFNYEIFPV-DEASKLKEVALFQSLE 1102

Query: 409  TLKMSCKEVVDERFWVMSKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVSYSIEE 468
            TL+MSCK+ V ERFWVMSKF KLK LEL GCE        D KMISLPMEM+EV YSIEE
Sbjct: 1103 TLRMSCKQAVKERFWVMSKFFKLKSLELFGCE--------DGKMISLPMEMNEVLYSIEE 1162

Query: 469  LTIRGCLQLVQVFGNDSYIQRCAN---------------LKNMNQMTATTFSKLVDLQVH 528
            LTIRGCLQLV V GND YIQRCAN               LKNMNQMTATTFSKLV LQV 
Sbjct: 1163 LTIRGCLQLVDVIGNDYYIQRCANLKKLKLYNLPKLMYVLKNMNQMTATTFSKLVYLQVG 1222

Query: 529  DCNGMINLFSPSVAKNLANLKSVEICNCREMTSIVAAKAEEEEENVEIVFNNLTNMEFDN 588
             CNGMINLFSPSVAKNLANL S+EI +C EM ++VAAKAEEEEENVEIVF+ LT MEF N
Sbjct: 1223 GCNGMINLFSPSVAKNLANLNSIEIYDCGEMRTVVAAKAEEEEENVEIVFSKLTGMEFHN 1282

Query: 589  LERLEWFYSRKCRFEFPILDTLRIDKCYDMKIFSYGI 608
            L  LE FY  KC  EFP+LDTLRI KC DMKIFSYGI
Sbjct: 1283 LAGLECFYPGKCTLEFPLLDTLRISKCDDMKIFSYGI 1309

BLAST of Cucsa.017500 vs. NCBI nr
Match: gi|778726034|ref|XP_011659043.1| (PREDICTED: probable disease resistance protein At4g27220 [Cucumis sativus])

HSP 1 Score: 164.5 bits (415), Expect = 6.2e-37
Identity = 179/677 (26.44%), Postives = 303/677 (44.76%), Query Frame = 1

Query: 6    SELKKLKVLVVLDCSNL-VVIHKNIISSMTKLEELDIQGRFTKWREKIVSVNPTKLSILL 65
            S   KL+ + + DC  L  V   NI +S+  L+ L I G   +  E I  +   K S   
Sbjct: 952  SSFSKLQTIEISDCKELRCVFPSNIATSLVFLDTLKIYG--CELLEMIFEIEKQKTS--- 1011

Query: 66   EGAEELVIVND-SKGFANNIF----KAIGN--GYPLLKYLKILENSETPHLRGNDFTSLE 125
             G  ++V +   S GF  N+     K + +   +P LK +K+    +   +    FT   
Sbjct: 1012 -GDTKVVPLRYLSLGFLKNLKYVWDKDVDDVVAFPNLKKVKVGRCPKLKIIFPASFTKY- 1071

Query: 126  WLVLKGMVMLESIVPRHS---PTNPFNKLKVVEITR--------CKQL--RNFFSLSIFK 185
               +K +  LE + P +    P +  +KLK V + +        CKQ     F+ +S F 
Sbjct: 1072 ---MKEIEELEMVEPFNYEIFPVDEASKLKEVALFQSLETLRMSCKQAVKERFWVMSKF- 1131

Query: 186  GLSNLREIKISECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTI 245
                L+ +++  C+   +++S+ +E    + +    ++  C   +L         IQ+  
Sbjct: 1132 --FKLKSLELFGCED-GKMISLPMEMNEVLYSIEELTIRGCL--QLVDVIGNDYYIQRCA 1191

Query: 246  VPFFDERRVSFPQLEDLSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVT 305
                + +++    L  L     N  +M      T+FSKL  +++  C  +  +F  ++  
Sbjct: 1192 ----NLKKLKLYNLPKLMYVLKNMNQM----TATTFSKLVYLQVGGCNGMINLFSPSVAK 1251

Query: 306  SLVSLDTLEINCCGLLEMIFEIEKPKTSCDTKVVVP-LRHLYLQVLPSLKYVWDKDGDDV 365
            +L +L+++EI  CG +  +   +  +   + ++V   L  +    L  L+  +   G   
Sbjct: 1252 NLANLNSIEIYDCGEMRTVVAAKAEEEEENVEIVFSKLTGMEFHNLAGLECFYP--GKCT 1311

Query: 366  VAFPNLKKVEVSRCPKLRSI-FPPSFTKYMKEIEELIVEDEPIFPVED-----EASKLKE 425
            + FP L  + +S+C  ++   +  + T  +K IE       P+ P +       A    E
Sbjct: 1312 LEFPLLDTLRISKCDDMKIFSYGITNTPTLKNIEIGEHNSLPVLPTQGINDIIHAFFTIE 1371

Query: 426  VALFQSLKTLKMSCKEVVDERFWVMSKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMS 485
            +   Q ++ LK+S K V          FS+LK LEL GCEDDD        ++ LP+EM 
Sbjct: 1372 IGSLQGIRNLKLSLKSVKKGFRQKPESFSELKSLELFGCEDDD--------IVCLPLEMK 1431

Query: 486  EVSYSIEELTIRGCLQLVQVFGNDSY-------IQRCANLKNM---------------NQ 545
            EV Y+ E++ I+   QLVQVF N+         +QRC  LKN+               ++
Sbjct: 1432 EVLYNTEKIEIKNGHQLVQVFENEELSRRNNDDVQRCGKLKNLTLSNLPKLMHVWKESSE 1491

Query: 546  MTATTFSKLVDLQV------------------------HDCNGMINLFSPSVAKNLANLK 605
            +T  +F  L  + +                         +CN M+NLFS SVA+ L NL+
Sbjct: 1492 VTTISFDSLEKINIRKCENLKCILPSSVTFLNLKFLWIRECNKMMNLFSSSVAETLRNLE 1551

Query: 606  SVEICNCREMTSIVAAKAEEEEENVEIVFNNLTNMEFDNLERLEWFYSRKCRFEFPILDT 608
            S+++ +C EM  IV  +   EEEN EIVF NL ++    L RL  F++ KC  +FP L+ 
Sbjct: 1552 SIDVSHCSEMRCIVTPEG-GEEENGEIVFKNLKSIILFGLPRLACFHNGKCMIKFPSLEI 1593

HSP 2 Score: 76.3 bits (186), Expect = 2.2e-10
Identity = 39/52 (75.00%), Postives = 43/52 (82.69%), Query Frame = 1

Query: 1   LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKI 53
           LPTSMSELK+LKVLVV  C  LVVIH NIISSMTKLEELDIQ  F +W E++
Sbjct: 611 LPTSMSELKQLKVLVVSHCFKLVVIHTNIISSMTKLEELDIQDCFKEWGEEV 662

HSP 3 Score: 814.7 bits (2103), Expect = 1.1e-232
Identity = 439/577 (76.08%), Postives = 479/577 (83.02%), Query Frame = 1

Query: 49   REKIVSVNPTKLSILLEGAEELVIVNDSKGFANNIFKAIGNGYPLLKYLKILENSETPHL 108
            + +IVSVN TKLSILLEG + L+I+NDSKGFAN+IFKAIGNGYPLLK L+I +NSETPHL
Sbjct: 743  KSQIVSVNGTKLSILLEGTKRLMILNDSKGFANDIFKAIGNGYPLLKCLEIHDNSETPHL 802

Query: 109  RGNDFTSLEWLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSN 168
            RGNDFTSL+ LVL  MVMLESI+PRHSP NPFNKLK ++I RC+QLRNFF LS+FKGLSN
Sbjct: 803  RGNDFTSLKRLVLDRMVMLESIIPRHSPINPFNKLKFIKIGRCEQLRNFFPLSVFKGLSN 862

Query: 169  LREIKISECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFF 228
            LR+I+I EC+MMEEIVSIEIED ITI TSPLTSL I  +NKL SFCSTKSSIQQTIVP F
Sbjct: 863  LRQIEIYECNMMEEIVSIEIEDHITIYTSPLTSLRIERVNKLTSFCSTKSSIQQTIVPLF 922

Query: 229  DERRVSFPQLEDLSIFRANNLEMLWHKNGTSFSKLQTVEISYCKKLRCVFPSNIVTSLVS 288
            DERRVSFP+L+ LSI RANNLEMLWHKNG+SFSKLQT+EIS CK+LRCVFPSNI TSLV 
Sbjct: 923  DERRVSFPELKYLSIGRANNLEMLWHKNGSSFSKLQTIEISDCKELRCVFPSNIATSLVF 982

Query: 289  LDTLEINCCGLLEMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPN 348
            LDTL+I  C LLEMIFEIEK KTS DTK VVPLR+L L  L +LKYVWDKD DDVVAFPN
Sbjct: 983  LDTLKIYGCELLEMIFEIEKQKTSGDTK-VVPLRYLSLGFLKNLKYVWDKDVDDVVAFPN 1042

Query: 349  LKKVEVSRCPKLRSIFPPSFTKYMKEIEEL-IVE--DEPIFPVEDEASKLKEVALFQSLK 408
            LKKV+V RCPKL+ IFP SFTKYMKEIEEL +VE  +  IFPV DEASKLKEVALFQSL+
Sbjct: 1043 LKKVKVGRCPKLKIIFPASFTKYMKEIEELEMVEPFNYEIFPV-DEASKLKEVALFQSLE 1102

Query: 409  TLKMSCKEVVDERFWVMSKFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVSYSIEE 468
            TL+MSCK+ V ERFWVMSKF KLK LEL GCE        D KMISLPMEM+EV YSIEE
Sbjct: 1103 TLRMSCKQAVKERFWVMSKFFKLKSLELFGCE--------DGKMISLPMEMNEVLYSIEE 1162

Query: 469  LTIRGCLQLVQVFGNDSYIQRCAN---------------LKNMNQMTATTFSKLVDLQVH 528
            LTIRGCLQLV V GND YIQRCAN               LKNMNQMTATTFSKLV LQV 
Sbjct: 1163 LTIRGCLQLVDVIGNDYYIQRCANLKKLKLYNLPKLMYVLKNMNQMTATTFSKLVYLQVG 1222

Query: 529  DCNGMINLFSPSVAKNLANLKSVEICNCREMTSIVAAKAEEEEENVEIVFNNLTNMEFDN 588
             CNGMINLFSPSVAKNLANL S+EI +C EM ++VAAKAEEEEENVEIVF+ LT MEF N
Sbjct: 1223 GCNGMINLFSPSVAKNLANLNSIEIYDCGEMRTVVAAKAEEEEENVEIVFSKLTGMEFHN 1282

Query: 589  LERLEWFYSRKCRFEFPILDTLRIDKCYDMKIFSYGI 608
            L  LE FY  KC  EFP+LDTLRI KC DMKIFSYGI
Sbjct: 1283 LAGLECFYPGKCTLEFPLLDTLRISKCDDMKIFSYGI 1309

BLAST of Cucsa.017500 vs. NCBI nr
Match: gi|700189056|gb|KGN44289.1| (hypothetical protein Csa_7G239020 [Cucumis sativus])

HSP 1 Score: 76.3 bits (186), Expect = 2.2e-10
Identity = 39/52 (75.00%), Postives = 43/52 (82.69%), Query Frame = 1

Query: 1   LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKI 53
           LPTSMSELK+LKVLVV  C  LVVIH NIISSMTKLEELDIQ  F +W E++
Sbjct: 611 LPTSMSELKQLKVLVVSHCFKLVVIHTNIISSMTKLEELDIQDCFKEWGEEV 662

HSP 2 Score: 53.9 bits (128), Expect = 1.2e-03
Identity = 61/233 (26.18%), Postives = 103/233 (44.21%), Query Frame = 1

Query: 17   LDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKIVSVNPTKLSILLEGAEELVIVNDS 76
            + C   V     ++S   KL+ L++ G       K++S+ P +++ +L   EEL I    
Sbjct: 1104 MSCKQAVKERFWVMSKFFKLKSLELFGCEDG---KMISL-PMEMNEVLYSIEELTIRG-- 1163

Query: 77   KGFANNIFKAIGNGYPLLKY--LKILENSETPHLRGNDFTSLEWLVLKGMVMLESIVPRH 136
                  +   IGN Y + +   LK L+    P L           VLK M  + +     
Sbjct: 1164 ---CLQLVDVIGNDYYIQRCANLKKLKLYNLPKLM---------YVLKNMNQMTATT--- 1223

Query: 137  SPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMMEEIVSI---EIEDQ 196
                 F+KL  +++  C  + N FS S+ K L+NL  I+I +C  M  +V+    E E+ 
Sbjct: 1224 -----FSKLVYLQVGGCNGMINLFSPSVAKNLANLNSIEIYDCGEMRTVVAAKAEEEEEN 1283

Query: 197  ITICTSPLTSLHICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLEDLSIF 245
            + I  S LT +   ++  L  F   K +++    P  D  R+S  + +D+ IF
Sbjct: 1284 VEIVFSKLTGMEFHNLAGLECFYPGKCTLE---FPLLDTLRIS--KCDDMKIF 1305

HSP 3 Score: 293.5 bits (750), Expect = 8.8e-76
Identity = 186/421 (44.18%), Postives = 258/421 (61.28%), Query Frame = 1

Query: 60   LSILLEGAEELVIVNDSKG-FANNIFKAIG-NGYPLLKYLKILE---NSETPHLRGNDFT 119
            L ILL+ +E L IV+DSKG F N IFK  G NGYP LKYL I++   NSE PHL G+DFT
Sbjct: 747  LEILLQRSERL-IVSDSKGNFINAIFKPNGLNGYPYLKYLWIIDENGNSEMPHLIGSDFT 806

Query: 120  SLEWLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKI 179
            SL++L++ GM  LE+IVPRH   +PF  +K + I  C Q+RN FS SIFK L +L+EI++
Sbjct: 807  SLKYLIIFGMKRLENIVPRHISISPFKSVKTIAIQFCGQIRNLFSFSIFKDLLDLQEIEV 866

Query: 180  SECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQ---QTIVPFFDER 239
              C  ME IV +EIEDQ+ ICTSPLTSL + +++KL SFC TK  +Q   Q+I+PFFD  
Sbjct: 867  INCGNMEGIVFMEIEDQLNICTSPLTSLQLENVDKLTSFC-TKGLVQESSQSIIPFFD-G 926

Query: 240  RVSFPQLEDLSIFRANNLEMLWHKNG----TSFSKLQTVEISYCKKLRCVFPSNIVTSLV 299
            +VSFP+L DLSI    NLE LWHKN      SF KLQ++ I  C +LRC+FPSN+  SL 
Sbjct: 927  QVSFPELNDLSIVGGYNLETLWHKNNNPTTNSFCKLQSIRIEQCTQLRCMFPSNMFASLT 986

Query: 300  SLDTLEINCCGLLEMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFP 359
            SL T++I  C  L+ IFEIE    S  T VV  L  L+L  LP+LK+VW KD   ++ FP
Sbjct: 987  SLHTIQIISCASLKRIFEIENQSFSVKT-VVWSLNELHLLDLPNLKHVWRKDIIKILTFP 1046

Query: 360  NLKKVEVSRCPKLRSIFPPSF--TKYMKEIEELIVED----EPIFPVEDEASKLKEVALF 419
            +LK+V++  C KL  ++  +   T+    +E + VE     + + P       LKE+ + 
Sbjct: 1047 SLKRVKIHGCTKLTHVWKDNTKETRSFDSLERIEVEKCKNLKYLLPSSITFLNLKELHIK 1106

Query: 420  QSLKTLKMSCKEVVDERFWVMS-KFSKLKRLELVGCEDDDDDDDDDDKMISLPMEMSEVS 462
            +    + +    V  +   + S K S  KR+    C  + D +++D+ ++   +   E+ 
Sbjct: 1107 KCNGMMNLFSSTVTKKLVNLRSIKVSYCKRMR---CMVEGDQEENDEIIVFKRLSRLELD 1160

BLAST of Cucsa.017500 vs. NCBI nr
Match: gi|659125615|ref|XP_008462777.1| (PREDICTED: disease resistance protein At4g27190-like [Cucumis melo])

HSP 1 Score: 120.6 bits (301), Expect = 1.0e-23
Identity = 107/430 (24.88%), Postives = 194/430 (45.12%), Query Frame = 1

Query: 235  FPQLEDLSIFRANNLEMLW--HKNGTSFSKLQTVEISYCKKLRCVFPSNI---------- 294
            F  L+ L IF    LE +   H + + F  ++T+ I +C ++R +F  +I          
Sbjct: 804  FTSLKYLIIFGMKRLENIVPRHISISPFKSVKTIAIQFCGQIRNLFSFSIFKDLLDLQEI 863

Query: 295  ----------VTSLVSLDTLEINCCGLLEMIFEIEKPKTSCDTKVVVP------------ 354
                      +  +   D L I    L  +  E     TS  TK +V             
Sbjct: 864  EVINCGNMEGIVFMEIEDQLNICTSPLTSLQLENVDKLTSFCTKGLVQESSQSIIPFFDG 923

Query: 355  ------LRHLYLQVLPSLKYVWDKDGDDVV-AFPNLKKVEVSRCPKLRSIFPPSFTKYMK 414
                  L  L +    +L+ +W K+ +    +F  L+ + + +C +LR +FP +    + 
Sbjct: 924  QVSFPELNDLSIVGGYNLETLWHKNNNPTTNSFCKLQSIRIEQCTQLRCMFPSNMFASLT 983

Query: 415  EIEELIVED----EPIFPVEDEASKLKEVALFQSLKTLKMSCKEVVDERFWVMSKFSKLK 474
             +  + +      + IF +E+++          S+KT+  S  E+       +     + 
Sbjct: 984  SLHTIQIISCASLKRIFEIENQSF---------SVKTVVWSLNEL---HLLDLPNLKHVW 1043

Query: 475  RLELVGCEDDDDDDDDDDKMISLPMEMSEVSYSIEELTIRGCLQLVQVFGNDSY------ 534
            R +++             K+++ P        S++ + I GC +L  V+ +++       
Sbjct: 1044 RKDII-------------KILTFP--------SLKRVKIHGCTKLTHVWKDNTKETRSFD 1103

Query: 535  ------IQRCANLKNMNQMTATTFSKLVDLQVHDCNGMINLFSPSVAKNLANLKSVEICN 594
                  +++C NLK +   ++ TF  L +L +  CNGM+NLFS +V K L NL+S+++  
Sbjct: 1104 SLERIEVEKCKNLKYLLP-SSITFLNLKELHIKKCNGMMNLFSSTVTKKLVNLRSIKVSY 1163

Query: 595  CREMTSIVAAKAEEEEENVEIVFNNLTNMEFDNLERLEWFYSRKCRFEFPILDTLRIDKC 608
            C+ M  +V  + ++EE +  IVF  L+ +E D L RL+ F+S KC  EFP L++L I +C
Sbjct: 1164 CKRMRCMV--EGDQEENDEIIVFKRLSRLELDYLPRLDSFHSGKCMLEFPCLESLVIKRC 1197

HSP 2 Score: 61.6 bits (148), Expect = 5.7e-06
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = 1

Query: 1   LPTSMSELKKLKVLVVLDCSNLVVIHKNIISSMTKLEELDIQGRFTKWREKI 53
           LP +MS+L +LKVL VL+CS L V+  N+ SSMTKLEEL +Q  F +W E++
Sbjct: 610 LPPTMSQLTQLKVLEVLNCSKLEVVPANVFSSMTKLEELKLQDSFCRWGEEV 661

HSP 3 Score: 50.4 bits (119), Expect = 1.3e-02
Identity = 47/167 (28.14%), Postives = 77/167 (46.11%), Query Frame = 1

Query: 91   YPLLKYLKILENSETPHL------RGNDFTSLEWLVLKGMVMLESIVPRHSPTNPFNKLK 150
            +P LK +KI   ++  H+          F SLE + ++    L+ ++P    +  F  LK
Sbjct: 1041 FPSLKRVKIHGCTKLTHVWKDNTKETRSFDSLERIEVEKCKNLKYLLPS---SITFLNLK 1100

Query: 151  VVEITRCKQLRNFFSLSIFKGLSNLREIKISECDMMEEIVSIEIEDQITICT-SPLTSLH 210
             + I +C  + N FS ++ K L NLR IK+S C  M  +V  + E+   I     L+ L 
Sbjct: 1101 ELHIKKCNGMMNLFSSTVTKKLVNLRSIKVSYCKRMRCMVEGDQEENDEIIVFKRLSRLE 1160

Query: 211  ICHMNKLASFCSTKSSIQQTIVPFFDERRVSFPQLEDLSIFRANNLE 251
            + ++ +L SF S K  ++             FP LE L I R   ++
Sbjct: 1161 LDYLPRLDSFHSGKCMLE-------------FPCLESLVIKRCPEMK 1191

HSP 4 Score: 290.4 bits (742), Expect = 7.5e-75
Identity = 167/335 (49.85%), Postives = 225/335 (67.16%), Query Frame = 1

Query: 60   LSILLEGAEELVIVNDSKG-FANNIFKAIGNGYPLLKYLKILE---NSETPHLRGNDFTS 119
            L ILL+ +E L IV+DSKG F N +FK  GNGYP LKYL +++   NSE  HL G+DFTS
Sbjct: 747  LEILLQRSERL-IVSDSKGNFINAMFKPNGNGYPCLKYLWMIDENGNSEMAHLIGSDFTS 806

Query: 120  LEWLVLKGMVMLESIVPRHSPTNPFNKLKVVEITRCKQLRNFFSLSIFKGLSNLREIKIS 179
            L++L++ GM  LE+IVPRH   +PF K+K + I  C Q+RN FS SIFK L +L+EI++ 
Sbjct: 807  LKYLIIFGMKRLENIVPRHISLSPFKKVKTIAIQFCGQIRNLFSFSIFKDLLDLQEIEVI 866

Query: 180  ECDMMEEIVSIEIEDQITICTSPLTSLHICHMNKLASFCSTKSSIQ---QTIVPFFDERR 239
             C  ME I+ +EI DQ+ IC+ PLTSL + +++KL SFC TK  IQ   Q+I+PFFD  +
Sbjct: 867  NCGKMEGIIFMEIGDQLNICSCPLTSLQLENVDKLTSFC-TKDLIQESSQSIIPFFD-GQ 926

Query: 240  VSFPQLEDLSIFRANNLEMLWHKNGT----SFSKLQTVEISYCKKLRCVFPSNIVTSLVS 299
            VSFP+L DLSI   NNLE LWHKN      SF KLQ++ I  C +LRC+FPSN++TSL S
Sbjct: 927  VSFPELNDLSIVGGNNLETLWHKNNNPTTGSFCKLQSIRIEQCTQLRCMFPSNMLTSLAS 986

Query: 300  LDTLEINCCGLLEMIFEIEKPKTSCDTKVVVPLRHLYLQVLPSLKYVWDKDGDDVVAFPN 359
            L T++I  C  L+ IFEIE    + DT V+  L  L+L  LP+LK VW KD   ++ FP+
Sbjct: 987  LHTIQIISCASLKRIFEIENQSFN-DTTVLWSLNELHLLNLPNLKQVWRKDIIKILTFPS 1046

Query: 360  LKKVEVSRCPKLRSIFPPS--FTKYMKEIEELIVE 382
            LK+V++  C KL  ++  +   T+    +E + VE
Sbjct: 1047 LKRVKIHGCTKLTHVWKDNNKVTRSFDSLERIEVE 1077