Cucsa.017480 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.017480 vs. Swiss-Prot
Match: HMA1_ARATH (Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis thaliana GN=HMA1 PE=2 SV=2)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 575/797 (72.15%), Postives = 654/797 (82.06%), Query Frame = 1

Query: 26  SQSRVGPVSRFSPLSSLIHLNRFPTRFK-KLSYPRFGHFRCAAEASGHRHGH-----HHG 85
           S  R+  V+ FS L     L + P R    L+ P       A E   H H H     HH 
Sbjct: 22  STLRLARVNSFSILPPKTLLRQKPLRISASLNLPPRSIRLRAVEDHHHDHHHDDEQDHHN 81

Query: 86  HHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLANYLREHLHMCCGSAALFVT 145
           HHHHHH  QH C       E +  QK    FA+AI W  LANYLREHLH+CC +AA+F+ 
Sbjct: 82  HHHHHH--QHGCCSVELKAE-SKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAAAMFLA 141

Query: 146 AAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFASIF 205
           AA  PYL P+P +K LQN F+ V FPLVGVSASLDAL DI+GGKVNIHVLMALAAFAS+F
Sbjct: 142 AAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAAFASVF 201

Query: 206 MGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLE 265
           MGN LEGGLLL MFN+AHIAEE+FT +SM+DVKELKES+PD AL+++V++ N+P+ SDL 
Sbjct: 202 MGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPNISDLS 261

Query: 266 HRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVRPLQIKVGERVPGGA 325
           ++ VPV  V+VGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEV+PL+ K G+RVPGGA
Sbjct: 262 YKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDRVPGGA 321

Query: 326 RNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVA 385
           RNLDGR+IVKATK W +STL++IVQLTEEA  NKPKLQRWLDEFGENYSKVVVVLS+A+A
Sbjct: 322 RNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVLSLAIA 381

Query: 386 LIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGG 445
            +GP LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGILLKG 
Sbjct: 382 FLGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKGA 441

Query: 446 HVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAV 505
            VLDA+ASCHT+AFDKTGTLTTGGL  KAIEPIYGH+ GG  S   +CCIP+CEKEALAV
Sbjct: 442 QVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVITCCIPNCEKEALAV 501

Query: 506 AAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLHGIKS-GIGGKLRKA 565
           AAAMEKGTTHPIGRAVVDHSVGKDLPSI VES EYFPGRGL AT++G+K+     +LRKA
Sbjct: 502 AAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEESRLRKA 561

Query: 566 SLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVID 625
           SLGS++FITS  KSE+ES+ IKDAV AS+YG +FVHAALSVDQKVTLIHLEDQPRPGV  
Sbjct: 562 SLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPRPGVSG 621

Query: 626 AIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGL 685
            IAEL+   +LRVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL HVK I+RE GGGL
Sbjct: 622 VIAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIAREAGGGL 681

Query: 686 IMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLI 745
           IMVGEGINDAPALAAATVGIVLA RASATA AVAD+LLL+D+I+GVPFC+AKSRQTTSL+
Sbjct: 682 IMVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQTTSLV 741

Query: 746 KQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRALNHPSWSWKQDLQN 805
           KQNV LALTSI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNSVR LN PSWSWKQD+ +
Sbjct: 742 KQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWKQDIVH 801

Query: 806 LIHDARSKLNTTPENSS 816
           LI+  RS+  T+  ++S
Sbjct: 802 LINKLRSQEPTSSSSNS 814

BLAST of Cucsa.017480 vs. Swiss-Prot
Match: CADA_LISMO (Probable cadmium-transporting ATPase OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) GN=cadA PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.2e-77
Identity = 184/601 (30.62%), Postives = 320/601 (53.24%), Query Frame = 1

Query: 189 LMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVN 248
           LM +A   +  +G   EG +++++F  + + E Y   ++   ++ L +  P  AL+    
Sbjct: 148 LMTIAIIGASIIGEWAEGSIVVILFAFSEVLERYSMDKARQSIRSLMDIAPKEALIR--- 207

Query: 249 DDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVRPLQ 308
                   D+E + + V D+++G  +++  G+ + +D  V +G + I    +TGE  P++
Sbjct: 208 ------RDDVE-QMIAVSDIQIGDIMIIKPGQKIAMDGVVIKGYSAINQSAITGESIPVE 267

Query: 309 IKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYS 368
            KV + V  G  N +G + VK TK  E++T+S+I+ L EEAQ  +   Q ++D+F + Y+
Sbjct: 268 KKVDDEVFAGTLNEEGLLEVKVTKHVEDTTISKIIHLVEEAQGERAPAQAFVDKFAKYYT 327

Query: 369 KVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYAIAIS 428
             ++++++ V ++ PL F   +         VY+ L L+V   PC+L  + P++   AI 
Sbjct: 328 PTIMLIALLVVVVPPLFFGGDWDTW------VYQGLSLLVVGCPCSLVISTPVSIVSAIG 387

Query: 429 SCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASC 488
           + A+ G+L+KGG  L+ +     +AFDKTGTLT G  V     P   H            
Sbjct: 388 NSAKNGVLVKGGIYLEEIGGLQAIAFDKTGTLTKGKPVVTDFIPYSEHMD---------- 447

Query: 489 CIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSV--GKDLPSISVESTEYFPGRGLIATLH 548
                E+ +L++  A+E  + HP+  A++  ++    D  SI +++     G+G+   ++
Sbjct: 448 -----EQNSLSIITALETMSQHPLASAIISKAMIDNVDYKSIEIDNFSSITGKGVKGEVN 507

Query: 549 GIKSGIGG-KLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQKVT 608
           GI   IG  KL ++SL     I     S+    + K    A  +G+E           + 
Sbjct: 508 GITYYIGSSKLFESSLEKSQSI-----SQTYQSLQKQGKTAMLFGTE--------SNILA 567

Query: 609 LIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKL 668
           +I + D+ R    + IA+L  LG    +MLTGD++ +A  +   +G++++   L PEDKL
Sbjct: 568 IIAVADEVRESSKEVIAQLHKLGIAHTIMLTGDNNDTAQFIGKEIGVSDIKAELMPEDKL 627

Query: 669 AHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGV 728
            ++KE+ ++  G + M+G+G+NDAPALAA+TVGI +    + TA   ADV L+ D +  +
Sbjct: 628 TYIKEL-KQTYGKVAMIGDGVNDAPALAASTVGIAMGGAGTDTALETADVALMGDDLKKL 687

Query: 729 PFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRA 786
           PF +  SR+T  +IKQN+T +L   LLA L  + G+L LW+ ++   G TLLV LN +R 
Sbjct: 688 PFIVNLSRKTLKIIKQNITFSLGIKLLALLLVLPGWLTLWIAIVADMGATLLVTLNGLRL 703

BLAST of Cucsa.017480 vs. Swiss-Prot
Match: CADA_LISMN (Probable cadmium-transporting ATPase OS=Listeria monocytogenes GN=cadA PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 2.1e-77
Identity = 195/680 (28.68%), Postives = 343/680 (50.44%), Query Frame = 1

Query: 110 FAEAIRWTDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGV 169
           F +   +TD  +++R++  +    + LF+ A  +   +       L N     A  + G 
Sbjct: 72  FTDPEHFTDHQSFIRKNWRLLL--SGLFI-AVGYASQIMNGEDFYLTNALFIFAIFIGGY 131

Query: 170 SASLDALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMI 229
           S   +   ++   +  +  LM +A   + F+G   EG +++++F ++   E Y   ++  
Sbjct: 132 SLFKEGFKNLLKFEFTMETLMTIAIIGAAFIGEWAEGSIVVILFAVSEALERYSMDKARQ 191

Query: 230 DVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVY 289
            ++ L +  P  ALV     D          R V V D+++G  +++  G+ + +D  V 
Sbjct: 192 SIRSLMDIAPKEALVRRSGTD----------RMVHVDDIQIGDIMIIKPGQKIAMDGHVV 251

Query: 290 QGCATITVEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEA 349
           +G + +    +TGE  P++  + + V  G  N +G + V  TK  E++T+S+I+ L EEA
Sbjct: 252 KGYSAVNQAAITGESIPVEKNIDDSVFAGTLNEEGLLEVAVTKRVEDTTISKIIHLVEEA 311

Query: 350 QLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVA 409
           Q  +   Q ++D F + Y+  ++V++  +A + PLLF         +   VY+ L ++V 
Sbjct: 312 QGERAPAQAFVDTFAKYYTPAIIVIAALIATVPPLLFGG------NWETWVYQGLSVLVV 371

Query: 410 ASPCALAAA-PLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTG-GLVFK 469
             PCAL  + P+A   AI + A+ G+L+KGG  L+ +     +AFDKTGTLT G  +V  
Sbjct: 372 GCPCALVVSTPVAIVTAIGNAAKNGVLVKGGVYLEEIGGLKAIAFDKTGTLTKGVPVVTD 431

Query: 470 AIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK--DLP 529
            IE      I  +K+                + AA+E+ + HP+  A++ +   +  DL 
Sbjct: 432 YIELTEATNIQHNKNYI--------------IMAALEQLSQHPLASAIIKYGETREMDLT 491

Query: 530 SISVESTEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRA 589
           SI+V       G+G+  T+ G    +G  +    L +  F  S  +  ++ ++      A
Sbjct: 492 SINVNDFTSITGKGIRGTVDGNTYYVGSPVLFKELLASQFTDSIHRQVSDLQL--KGKTA 551

Query: 590 STYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKV 649
             +G+          + ++++ + D+ R      I  L  LG  + +MLTGD+ ++A  +
Sbjct: 552 MLFGTN--------QKLISIVAVADEVRSSSQHVIKRLHELGIEKTIMLTGDNQATAQAI 611

Query: 650 ANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRAS 709
              VG++E+   L P+DKL ++K++    G  + MVG+GINDAPALAAATVGI +    +
Sbjct: 612 GQQVGVSEIEGELMPQDKLDYIKQLKINFGK-VAMVGDGINDAPALAAATVGIAMGGAGT 671

Query: 710 ATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWL 769
            TA   ADV L+ D +  +PF +  SR+T  +IKQN+T +L   L+A L  + G+L LW+
Sbjct: 672 DTAIETADVALMGDDLQKLPFTVKLSRKTLQIIKQNITFSLVIKLIALLLVIPGWLTLWI 707

Query: 770 TVLLHEGGTLLVCLNSVRAL 786
            ++   G TLLV LN +R +
Sbjct: 732 AIMADMGATLLVTLNGLRLM 707

BLAST of Cucsa.017480 vs. Swiss-Prot
Match: CADA_STAAR (Probable cadmium-transporting ATPase OS=Staphylococcus aureus (strain MRSA252) GN=cadA PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 5.7e-75
Identity = 179/605 (29.59%), Postives = 319/605 (52.73%), Query Frame = 1

Query: 185 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 244
           ++  LM +A   +  +G   E  +++V+F ++   E +   ++   ++ L +  P  ALV
Sbjct: 160 DMKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALV 219

Query: 245 LDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 304
           +    + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 220 MRNGQEIM----------IHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGES 279

Query: 305 RPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 364
            P+   V + V  G  N +G + VK TK  E++T+S+I+ L EEAQ  +   Q ++D+F 
Sbjct: 280 VPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFA 339

Query: 365 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 424
           + Y+ +++V++  VA++ PL F   +         VY+ L ++V   PCAL  + P++  
Sbjct: 340 KYYTPIIMVIAALVAVVPPLFFGGSWDTW------VYQGLAVLVVGCPCALVISTPISIV 399

Query: 425 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQ 484
            AI + A+KG+L+KGG  L+ + +   +AFDKTGTLT G      +  +   K+  D+ +
Sbjct: 400 SAIGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLTKG------VPVVTDFKVLNDQVE 459

Query: 485 FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLP--SISVESTEYFPGRGLI 544
                    EKE  ++  A+E  + HP+  A++  +   ++    + VE      GRG+ 
Sbjct: 460 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVEDFTSITGRGIQ 519

Query: 545 ATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQ 604
             + G    IG       L   DF   F   EN+ +++++  + +          +  DQ
Sbjct: 520 GNIDGTTYYIGSPRLFKELNVSDFSLEF---ENKVKVLQNQGKTAMI--------IGTDQ 579

Query: 605 KVT-LIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 664
            +  +I + D+ R    + I +L  LG  + +MLTGD+  +A  +   VG++++   L P
Sbjct: 580 TILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSELMP 639

Query: 665 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 724
           +DKL ++K++  E G  + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D 
Sbjct: 640 QDKLDYIKKMKAEHGN-VAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGDD 699

Query: 725 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 784
           +S +PF +  SR+T ++IK N+T A+   ++A L  + G+L LW+ +L   G T+LV LN
Sbjct: 700 LSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALN 721

Query: 785 SVRAL 786
           S+R +
Sbjct: 760 SLRLM 721

BLAST of Cucsa.017480 vs. Swiss-Prot
Match: CADA1_STAAU (Probable cadmium-transporting ATPase OS=Staphylococcus aureus GN=cadA PE=3 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.3e-74
Identity = 185/605 (30.58%), Postives = 320/605 (52.89%), Query Frame = 1

Query: 185 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 244
           ++  LM +A   +  +G   E  +++++F ++   E +   +S   ++ L +  P  ALV
Sbjct: 161 DMKTLMTVAVIGATIIGKWAEASIVVILFAISEALERFSMDRSRQSIRSLMDIAPKEALV 220

Query: 245 LDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 304
                + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 221 RRNGQEII----------IHVDDIAVGDIMIVKPGEKIAMDGIIVNGLSAVNQAAITGES 280

Query: 305 RPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 364
            P+   V + V  G  N +G I VK TK  E++T+++I+ L EEAQ  +   Q ++D+F 
Sbjct: 281 VPVSKAVDDEVFAGTLNEEGLIEVKITKYVEDTTITKIIHLVEEAQGERAPAQAFVDKFA 340

Query: 365 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 424
           + Y+ +++V++  VA++ PL F   +         VY+ L ++V   PCAL  + P++  
Sbjct: 341 KYYTPIIMVIAALVAVVPPLFFGGSWDTW------VYQGLAVLVVGCPCALVISTPISIV 400

Query: 425 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQ 484
            AI + A+KG+L+KGG  L+ + +  TVAFDKTGTLT G  V    E +       D+ +
Sbjct: 401 SAIGNAAKKGVLVKGGVYLEKLGAIKTVAFDKTGTLTKGVPVVTDFEVL------NDQVE 460

Query: 485 FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEY--FPGRGLI 544
                    EKE  ++  A+E  + HP+  A++  +   ++P  +V+  E+    GRG+ 
Sbjct: 461 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNIPYSNVQVEEFTSITGRGIK 520

Query: 545 ATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVR-ASTYGSEFVHAALSVD 604
             ++G    IG       L   DF   F   EN  +++++  + A   G+E     L V 
Sbjct: 521 GIVNGTTYYIGSPKLFKELNVSDFSLGF---ENNVKILQNQGKTAMIIGTE--KTILGV- 580

Query: 605 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 664
                I + D+ R    + I +L  LG  + +MLTGD+  +A  +   VG++++   L P
Sbjct: 581 -----IAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTANAIGTHVGVSDIQSELMP 640

Query: 665 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 724
           +DKL ++K++  E    + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D 
Sbjct: 641 QDKLDYIKKMQSEYDN-VAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGDD 700

Query: 725 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 784
           +S +PF +  SR+T ++IK N+T A+   ++A L  + G+L LW+ +L   G T+LV LN
Sbjct: 701 LSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALN 722

Query: 785 SVRAL 786
           S+R +
Sbjct: 761 SLRLM 722

BLAST of Cucsa.017480 vs. TrEMBL
Match: A0A0A0LN89_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G433360 PE=3 SV=1)

HSP 1 Score: 1622.1 bits (4199), Expect = 0.0e+00
Identity = 817/823 (99.27%), Postives = 817/823 (99.27%), Query Frame = 1

Query: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60
           MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120
           GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG     L VLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG-----LKVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 824
           SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS
Sbjct: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 818

BLAST of Cucsa.017480 vs. TrEMBL
Match: A0A0D2SPC0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G218200 PE=3 SV=1)

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 610/838 (72.79%), Postives = 690/838 (82.34%), Query Frame = 1

Query: 1   MDTLFVPIT-FSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPR 60
           M+T  + +T FS L  PL        +     P+  FSPLS         TR+K L  P 
Sbjct: 1   METHSISLTNFSPLTRPLRPSRLRRLNSFHFKPLF-FSPLS---------TRYKSLFLPL 60

Query: 61  FGH---FRCAAEAS-----------GHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQK 120
             H    RC A              GH H H H HHHHHH   HH        +L G QK
Sbjct: 61  NSHTIRIRCVANHGHHHHHEHDLDHGHGHDHDHDHHHHHHHHHHHHGSG----QLNGPQK 120

Query: 121 AFVRFAEAIRWTDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFP 180
           A + FA+AIRW DLAN+LREHLH+CC + ALF+ AAAFPYLVPKPAVKPLQN F+ +AFP
Sbjct: 121 AVIGFAKAIRWMDLANFLREHLHLCCCATALFIAAAAFPYLVPKPAVKPLQNSFLVLAFP 180

Query: 181 LVGVSASLDALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTK 240
           LVGVSA+LDA+TDI+GGKVNIHVLMALAAFAS+FMGN LEGGLLL MFN+AHIAEE+FT 
Sbjct: 181 LVGVSAALDAITDIAGGKVNIHVLMALAAFASVFMGNALEGGLLLAMFNLAHIAEEFFTS 240

Query: 241 QSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVD 300
           +SMIDVKELKE++PD ALVL+++DDNLP+ SDL +R +PV DV+VGSYILV  GE+VPVD
Sbjct: 241 RSMIDVKELKENYPDSALVLNLDDDNLPNVSDLSYRSIPVHDVEVGSYILVTTGEAVPVD 300

Query: 301 CEVYQGCATITVEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQL 360
           CEV+ G ATIT+EHLTGE++PL+ K G+R+PGGARNLDGR+IVK  KTW+ESTLSRIVQL
Sbjct: 301 CEVFHGSATITIEHLTGEIKPLEAKAGDRIPGGARNLDGRMIVKVLKTWKESTLSRIVQL 360

Query: 361 TEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG 420
           TEEAQLNKPKLQRWLDEFGE YSKVVVVLSVA+A++GP LFKWPFI T   RGS+YRALG
Sbjct: 361 TEEAQLNKPKLQRWLDEFGEQYSKVVVVLSVAIAVLGPFLFKWPFISTAVCRGSIYRALG 420

Query: 421 LMVAASPCALAAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLV 480
           LMVAASPCALA APLAYA A+SSCARKGILLKGG VLDA+ASCHTVAFDKTGTLTTGGL+
Sbjct: 421 LMVAASPCALAVAPLAYATAVSSCARKGILLKGGQVLDALASCHTVAFDKTGTLTTGGLM 480

Query: 481 FKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLP 540
           FKAIEPIYGH IG  K+ F SCC+P+CE EALAVAAAMEKGTTHPIGRAVVDHS+GKDLP
Sbjct: 481 FKAIEPIYGHIIGNKKTNFTSCCVPNCEVEALAVAAAMEKGTTHPIGRAVVDHSIGKDLP 540

Query: 541 SISVESTEYFPGRGLIATLHGIKSGI-GGKLRKASLGSVDFITSFCKSENESRMIKDAVR 600
           S+SVES EYFPG+GLIATL+  +SG  GGK+ KASLGS++FITS CKSE +SRMI+ AV 
Sbjct: 541 SVSVESFEYFPGKGLIATLNSAESGTRGGKMLKASLGSIEFITSLCKSEVKSRMIRAAVN 600

Query: 601 ASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWK 660
           AS+YG++FVHAALSVD+KVTLIHLED+PRPGV+D I+EL+   K+RVMMLTGDH  SAW+
Sbjct: 601 ASSYGTDFVHAALSVDEKVTLIHLEDRPRPGVLDVISELKDKAKVRVMMLTGDHKLSAWR 660

Query: 661 VANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRA 720
           VANAVGINEVY SLKPEDKL HVK IS +MGGGLIMVGEGINDAPALAAATVGIVLAHRA
Sbjct: 661 VANAVGINEVYCSLKPEDKLNHVKRISGDMGGGLIMVGEGINDAPALAAATVGIVLAHRA 720

Query: 721 SATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLW 780
           SATA AVADVLLLQD+ISGVPF IAK+RQTTSL+KQNV LALT I+LASLPSVLGFLPLW
Sbjct: 721 SATAIAVADVLLLQDNISGVPFSIAKARQTTSLVKQNVALALTCIILASLPSVLGFLPLW 780

Query: 781 LTVLLHEGGTLLVCLNSVRALNHPSWSWKQDLQNLIHDARSKLNTTPEN-SSGTIQTA 822
           LTVLLHEGGTLLVCLNS+RALN PSWSW QDL+NLI   +SKL     N +S TIQTA
Sbjct: 781 LTVLLHEGGTLLVCLNSIRALNDPSWSWGQDLRNLIGKLKSKLALLRHNATSSTIQTA 824

BLAST of Cucsa.017480 vs. TrEMBL
Match: M5W7S9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001453mg PE=3 SV=1)

HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 611/828 (73.79%), Postives = 693/828 (83.70%), Query Frame = 1

Query: 6   VPITFSELPHPLL------TCNTNAASQSRV---GPVSRFSPLSSLIHLNRFPTRFKKLS 65
           +PI  S   H LL      T   N+ S  +     P+ RF PL  L +            
Sbjct: 9   IPIRASNHHHSLLLSHHPTTTRLNSPSSPKPIIRAPLFRFKPLPPLRNSTTL-------- 68

Query: 66  YPRFGHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRW 125
                  RC A A  H H HHH HHHHHH   HH S      ELTG QK FVRFA+A+RW
Sbjct: 69  -------RCTAYACDHDH-HHHDHHHHHHHHHHHGSD-----ELTGPQKQFVRFAKAVRW 128

Query: 126 TDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 185
           TDLA++LREHL +C  S  LF+ AAA PYL+PK AVKP+QN FI +AFPLVGVSA+LDAL
Sbjct: 129 TDLADFLREHLQLCFCSTTLFLAAAACPYLMPKLAVKPMQNAFILIAFPLVGVSAALDAL 188

Query: 186 TDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 245
           TDISGGKVNIHVLMALAAFAS+FMGN LEGGLLL MFN+AHIAEEYFT +SMIDVKELKE
Sbjct: 189 TDISGGKVNIHVLMALAAFASVFMGNALEGGLLLAMFNLAHIAEEYFTSRSMIDVKELKE 248

Query: 246 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATIT 305
           ++PDFALVLD+ND+ LP+TS+L +++VPV D++VGS+ILVGAGESVPVDCEV+QG ATIT
Sbjct: 249 NYPDFALVLDINDEELPNTSNLAYKQVPVHDIQVGSFILVGAGESVPVDCEVFQGNATIT 308

Query: 306 VEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 365
           +EHLTGE++PL+  VG+RVPGGARNLDGRIIVKATKTW+ESTLSRIVQLTEEAQLNKPKL
Sbjct: 309 IEHLTGEIKPLETTVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVQLTEEAQLNKPKL 368

Query: 366 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 425
           QRWLD+FGE YSKVVVVLS A+AL+GP LFKWPFI T   RGSVYRALGLMVAASPCALA
Sbjct: 369 QRWLDQFGEQYSKVVVVLSAAIALLGPFLFKWPFIGTSACRGSVYRALGLMVAASPCALA 428

Query: 426 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 485
            APLAYA AISSCA+KGILLKGGHVLDA+ASCHT+AFDKTGTLTTGGL FKAIEPIYGH+
Sbjct: 429 VAPLAYATAISSCAKKGILLKGGHVLDALASCHTIAFDKTGTLTTGGLAFKAIEPIYGHR 488

Query: 486 IGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFP 545
           +  + S F+SCC PSCEKEALAVAAAMEKGTTHPIGRAVVDHS GKDLPS+SVES EYFP
Sbjct: 489 MTNNISDFSSCCAPSCEKEALAVAAAMEKGTTHPIGRAVVDHSEGKDLPSVSVESFEYFP 548

Query: 546 GRGLIATLHGIKSGIGG-KLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHA 605
           GRGLIATL+GI+ G GG KL KASLGSVDFITS C+SE+ S+ IK+AV AS+YG+EFV A
Sbjct: 549 GRGLIATLNGIELGTGGDKLLKASLGSVDFITSLCRSEDASKKIKEAVNASSYGTEFVRA 608

Query: 606 ALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVY 665
           ALSV++KVTLIHLED+PRPGV D I EL+   KLRVMMLTGDH+SSAW+VANAVGINEVY
Sbjct: 609 ALSVNEKVTLIHLEDRPRPGVSDVIEELRDEAKLRVMMLTGDHESSAWRVANAVGINEVY 668

Query: 666 YSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVL 725
            SLKPEDKL+HVK++SR+MGGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVL
Sbjct: 669 SSLKPEDKLSHVKDVSRDMGGGLIMVGEGINDAPALAAATVGIVLAQRASATATAVADVL 728

Query: 726 LLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTL 785
           LL+D+IS VPFCIAKSRQTTSL+KQ+V LAL+ I+LASLPSVLGFLPLWLTVLLHEGGTL
Sbjct: 729 LLRDNISVVPFCIAKSRQTTSLVKQSVGLALSCIVLASLPSVLGFLPLWLTVLLHEGGTL 788

Query: 786 LVCLNSVRALNHPSWSWKQDLQNLIHDARSKLNTTPE--NSSGTIQTA 822
           +VCLNS+RALNHP+WSW+QDL +L+ + +S+L    +   SS T Q A
Sbjct: 789 VVCLNSIRALNHPTWSWRQDLWHLVCELKSRLVLPKKLNTSSNTAQPA 815

BLAST of Cucsa.017480 vs. TrEMBL
Match: W9QYY4_9ROSA (Putative cadmium/zinc-transporting ATPase HMA1 OS=Morus notabilis GN=L484_009289 PE=3 SV=1)

HSP 1 Score: 1182.5 bits (3058), Expect = 0.0e+00
Identity = 615/834 (73.74%), Postives = 696/834 (83.45%), Query Frame = 1

Query: 13  LPHPLLTCNTNAASQSRVGPVSRFSPLSSLIH---------LNRFPTRFKKLSYPRFGHF 72
           LP+P+     N+   SR   V   SP   L H            FPTR    S+  F +F
Sbjct: 4   LPYPIGVGKYNSLLLSRKRSVPVNSPRPVLFHSGLRFSSVRFRTFPTR----SF-NFSNF 63

Query: 73  RCAAEASGHRHGHHH----GHHHHHHGCQHH-------CSGDSDGVELTGAQKAFVRFAE 132
           RCAA+A+ H H HHH     H H HH  QHH       C    +  EL  +QKAF+RFA+
Sbjct: 64  RCAAKAADHGHNHHHHQLEDHDHDHHDLQHHHHHHCGNCGNCGEVSELKESQKAFLRFAK 123

Query: 133 AIRWTDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSAS 192
           A+RWT+LAN+LRE+L +CC SAALFV AAAFP+L+PKPAVKPLQN F+ VAFPLVGVSAS
Sbjct: 124 AVRWTELANFLRENLLLCCVSAALFVAAAAFPHLLPKPAVKPLQNAFLLVAFPLVGVSAS 183

Query: 193 LDALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVK 252
           LDAL DISGGKVNIHVLMALAAFAS+FMGN LEGGLLL MFN+AHIAEEYFT +SMIDVK
Sbjct: 184 LDALIDISGGKVNIHVLMALAAFASVFMGNALEGGLLLAMFNLAHIAEEYFTSRSMIDVK 243

Query: 253 ELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGC 312
           ELKE+HP+FALVLD+NDD LP+T DL ++RVPV +V++GSYIL+GAGESVPVDCEV++G 
Sbjct: 244 ELKENHPEFALVLDMNDDRLPNTFDLAYKRVPVHNVEMGSYILIGAGESVPVDCEVFEGS 303

Query: 313 ATITVEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLN 372
           ATIT EHLTGEV+PL+IKVG+R+PGGARNLDGR+IVKATKTW+ESTLSRIVQLTEEA+ N
Sbjct: 304 ATITTEHLTGEVKPLEIKVGDRIPGGARNLDGRMIVKATKTWKESTLSRIVQLTEEARSN 363

Query: 373 KPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASP 432
           KPKLQRWLD+FGENYSKVVVVLSVA+ALIGP +FKWPF  T   RGSVYRALGLMVAASP
Sbjct: 364 KPKLQRWLDQFGENYSKVVVVLSVAIALIGPFVFKWPFFGTSACRGSVYRALGLMVAASP 423

Query: 433 CALAAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI 492
           CALA APLAYA AISSCARKGILLKGGHVLDA+ASCHT+AFDKTGTLTTG LVFKAIEPI
Sbjct: 424 CALAVAPLAYATAISSCARKGILLKGGHVLDALASCHTIAFDKTGTLTTGKLVFKAIEPI 483

Query: 493 YGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVEST 552
           YGH++  + S F +CC P+CEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPS+SVES 
Sbjct: 484 YGHQVRHNNSNFTACCAPNCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSVSVESF 543

Query: 553 EYFPGRGLIATLHGIKSGI---GGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYG 612
           EYFPGRGL+ATL+  +S      GKL +ASLGSVDFITS CKS+ +S  IKDAV AS+YG
Sbjct: 544 EYFPGRGLVATLNSFQSQSETGDGKLLRASLGSVDFITSRCKSKYDSEKIKDAVNASSYG 603

Query: 613 SEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAV 672
           SEFV AALS    VTLIHLED+PRPGV+D I ELQ  GKL VMMLTGDH SSA +VANAV
Sbjct: 604 SEFVRAALS----VTLIHLEDRPRPGVVDVIRELQDQGKLHVMMLTGDHKSSALRVANAV 663

Query: 673 GINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATAT 732
           GINEV+ SLKPEDKL+HVKEISR+MGGGLIMVGEGINDAPALAAAT+GIVLA RASATA 
Sbjct: 664 GINEVHCSLKPEDKLSHVKEISRDMGGGLIMVGEGINDAPALAAATIGIVLAQRASATAV 723

Query: 733 AVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLL 792
           AVADVLLL+D+ISGVPFCIAKSRQTTSLIKQNV LALTSI+LASLPSV+GFLPLWLTVLL
Sbjct: 724 AVADVLLLRDNISGVPFCIAKSRQTTSLIKQNVALALTSIVLASLPSVMGFLPLWLTVLL 783

Query: 793 HEGGTLLVCLNSVRALNHPSWSWKQDLQNLIHDARSKLNTTPEN--SSGTIQTA 822
           HEGGTLLVCLNS+RALN+P+WSW+QD  +LI++ + +L    E+  S+G IQ A
Sbjct: 784 HEGGTLLVCLNSIRALNNPTWSWRQDFWHLINELKCRLVFFREHNTSAGNIQAA 828

BLAST of Cucsa.017480 vs. TrEMBL
Match: A0A061DNL1_THECC (Heavy metal atpase 1 OS=Theobroma cacao GN=TCM_000697 PE=3 SV=1)

HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 606/813 (74.54%), Postives = 680/813 (83.64%), Query Frame = 1

Query: 22  TNAASQSRVGPVSRFSPLSSLIHLNRFP----------TRFKKLSYP-RFGHFRCAAEAS 81
           T++ S ++  P+SR  P   L  +N F            R K LS   R  H R    A+
Sbjct: 3   THSISLTKFSPLSR--PRCRLSRVNTFKFKPFILSPVIIRHKSLSLTLRSPHLRIRCVAN 62

Query: 82  GHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLANYLREHLHMC 141
            H H HH  HHHHH    HH     D  +L+G Q A + FA+A+RW DLANYLREHL +C
Sbjct: 63  -HDHDHHFNHHHHHDHDHHH-HHHHDSAKLSGPQGAVIGFAKAVRWMDLANYLREHLQLC 122

Query: 142 CGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGKVNIHVLM 201
           C + ALF+ AAA PYL+PKPAVKPLQN F+ VAFPLVGVSA+LDA+TDI+GGKVNIHVLM
Sbjct: 123 CCATALFLAAAACPYLLPKPAVKPLQNSFLFVAFPLVGVSAALDAITDIAGGKVNIHVLM 182

Query: 202 ALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDD 261
           ALAAFAS+FMGN LEGGLLL MFN+AHIAEE+FT +SM+DVKELKE++PD  LVL+++DD
Sbjct: 183 ALAAFASVFMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKENYPDSVLVLNLDDD 242

Query: 262 NLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVRPLQIK 321
           NLP+ S+L +R VPV DV+VGSYILVG GE+VPVDCEV+QG ATIT EHLTGE++PL+ K
Sbjct: 243 NLPNVSNLSYRNVPVHDVEVGSYILVGTGEAVPVDCEVFQGSATITTEHLTGEIKPLEAK 302

Query: 322 VGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKV 381
           VG+R+PGGARNLDGR+IVK TKTW+ESTLSRIVQLTEEAQLNKPKLQRWLDEFGE YSKV
Sbjct: 303 VGDRIPGGARNLDGRMIVKVTKTWKESTLSRIVQLTEEAQLNKPKLQRWLDEFGERYSKV 362

Query: 382 VVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCA 441
           VVVLSV +A++GP LFKWPFI T   RGS+YRALGLMVAASPCALA APLAYAIA+SSCA
Sbjct: 363 VVVLSVTIAVLGPFLFKWPFISTAVCRGSIYRALGLMVAASPCALAVAPLAYAIAVSSCA 422

Query: 442 RKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCIP 501
           RKGILLKGG VLDA+ASCHTVAFDKTGTLTTGGL+FKAIEPIYGH IG  K+ F SCCIP
Sbjct: 423 RKGILLKGGQVLDALASCHTVAFDKTGTLTTGGLMFKAIEPIYGHFIGNKKTNFTSCCIP 482

Query: 502 SCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLHGIKSG 561
           SCE EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPS+SVES EYFPGRGLIATL+  KSG
Sbjct: 483 SCEVEALAVAAAMEKGTTHPIGRAVVDHSIGKDLPSVSVESFEYFPGRGLIATLNSAKSG 542

Query: 562 I-GGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQKVTLIHLE 621
              GK+ KASLGSV+FITS CKSE+ESR I+ AV ASTYGS+FVHAALSV++KVTLIHLE
Sbjct: 543 TREGKMLKASLGSVEFITSLCKSEDESRKIRAAVNASTYGSDFVHAALSVNEKVTLIHLE 602

Query: 622 DQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKE 681
           D+PRPGV+D I+EL+   KLRVMMLTGDH SSAW+VANAVGINEVY SLKPEDKL HVK 
Sbjct: 603 DRPRPGVLDVISELKDQAKLRVMMLTGDHKSSAWRVANAVGINEVYCSLKPEDKLNHVKR 662

Query: 682 ISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIA 741
           ISRE GGGL MVGEGINDAPALAAATVGIVLAHRASATA AVADVLLL+D+IS VPF IA
Sbjct: 663 ISRETGGGLSMVGEGINDAPALAAATVGIVLAHRASATAIAVADVLLLRDNISCVPFSIA 722

Query: 742 KSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRALNHPS 801
           K+RQTTSL+KQNV LALT I+LASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRALN PS
Sbjct: 723 KARQTTSLVKQNVALALTCIILASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRALNDPS 782

Query: 802 WSWKQDLQNLIHDARSKLNTTPEN-SSGTIQTA 822
           WSWKQDL +LI   +S+L     N SS T Q A
Sbjct: 783 WSWKQDLLHLISKLKSELTLLRHNTSSSTTQPA 811

BLAST of Cucsa.017480 vs. TAIR10
Match: AT4G37270.1 (AT4G37270.1 heavy metal atpase 1)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 575/797 (72.15%), Postives = 654/797 (82.06%), Query Frame = 1

Query: 26  SQSRVGPVSRFSPLSSLIHLNRFPTRFK-KLSYPRFGHFRCAAEASGHRHGH-----HHG 85
           S  R+  V+ FS L     L + P R    L+ P       A E   H H H     HH 
Sbjct: 22  STLRLARVNSFSILPPKTLLRQKPLRISASLNLPPRSIRLRAVEDHHHDHHHDDEQDHHN 81

Query: 86  HHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLANYLREHLHMCCGSAALFVT 145
           HHHHHH  QH C       E +  QK    FA+AI W  LANYLREHLH+CC +AA+F+ 
Sbjct: 82  HHHHHH--QHGCCSVELKAE-SKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAAAMFLA 141

Query: 146 AAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFASIF 205
           AA  PYL P+P +K LQN F+ V FPLVGVSASLDAL DI+GGKVNIHVLMALAAFAS+F
Sbjct: 142 AAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAAFASVF 201

Query: 206 MGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLE 265
           MGN LEGGLLL MFN+AHIAEE+FT +SM+DVKELKES+PD AL+++V++ N+P+ SDL 
Sbjct: 202 MGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPNISDLS 261

Query: 266 HRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVRPLQIKVGERVPGGA 325
           ++ VPV  V+VGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEV+PL+ K G+RVPGGA
Sbjct: 262 YKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDRVPGGA 321

Query: 326 RNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVA 385
           RNLDGR+IVKATK W +STL++IVQLTEEA  NKPKLQRWLDEFGENYSKVVVVLS+A+A
Sbjct: 322 RNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVLSLAIA 381

Query: 386 LIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGG 445
            +GP LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGILLKG 
Sbjct: 382 FLGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKGA 441

Query: 446 HVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAV 505
            VLDA+ASCHT+AFDKTGTLTTGGL  KAIEPIYGH+ GG  S   +CCIP+CEKEALAV
Sbjct: 442 QVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVITCCIPNCEKEALAV 501

Query: 506 AAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLHGIKS-GIGGKLRKA 565
           AAAMEKGTTHPIGRAVVDHSVGKDLPSI VES EYFPGRGL AT++G+K+     +LRKA
Sbjct: 502 AAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEESRLRKA 561

Query: 566 SLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVID 625
           SLGS++FITS  KSE+ES+ IKDAV AS+YG +FVHAALSVDQKVTLIHLEDQPRPGV  
Sbjct: 562 SLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPRPGVSG 621

Query: 626 AIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGL 685
            IAEL+   +LRVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL HVK I+RE GGGL
Sbjct: 622 VIAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIAREAGGGL 681

Query: 686 IMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLI 745
           IMVGEGINDAPALAAATVGIVLA RASATA AVAD+LLL+D+I+GVPFC+AKSRQTTSL+
Sbjct: 682 IMVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQTTSLV 741

Query: 746 KQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRALNHPSWSWKQDLQN 805
           KQNV LALTSI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNSVR LN PSWSWKQD+ +
Sbjct: 742 KQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWKQDIVH 801

Query: 806 LIHDARSKLNTTPENSS 816
           LI+  RS+  T+  ++S
Sbjct: 802 LINKLRSQEPTSSSSNS 814

BLAST of Cucsa.017480 vs. TAIR10
Match: AT4G30110.1 (AT4G30110.1 heavy metal atpase 2)

HSP 1 Score: 229.2 bits (583), Expect = 9.4e-60
Identity = 179/634 (28.23%), Postives = 310/634 (48.90%), Query Frame = 1

Query: 159 FIAVAFPLVGVSASL-DALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 218
           ++AVA  + G+   L  A+  ++  +++I++L+ +   A+I M +  E  +++ +F +A 
Sbjct: 112 WLAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAE 171

Query: 219 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVG 278
             +   + ++   ++ L    P  A++ +  ++            V V ++K  + I V 
Sbjct: 172 WLQSRASYKASAVMQSLMSLAPQKAVIAETGEE------------VEVDELKTNTVIAVK 231

Query: 279 AGESVPVDCEVYQGCATITVEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEES 338
           AGE++P+D  V  G   +  + LTGE  P+       V  G  NL+G I V  T   E+ 
Sbjct: 232 AGETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDC 291

Query: 339 TLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 398
            ++++ +L EEAQ +K + QR++D+  + Y+  ++++S+    I P   K         +
Sbjct: 292 VVAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAI-PFALK-----VHNLK 351

Query: 399 GSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 458
             V+ AL ++V+A PC L  + P+A   A++  A  G+L+KG   L+ +A    VAFDKT
Sbjct: 352 HWVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKT 411

Query: 459 GTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 518
           GT+T G  +                  F S       +  L   ++ E  ++HP+  AVV
Sbjct: 412 GTITRGEFIV---------------MDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAVV 471

Query: 519 DH--SVGKDLPSISVESTEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSEN 578
           D+  SV  +    +VE  + FPG G+   + G +  IG K   +  G        C S  
Sbjct: 472 DYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRAG--------CLSVP 531

Query: 579 ESRMIKDAVRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMML 638
           +  +  D     T G  +V   L+      + +L D  R GV  A+ EL+ LG +++ ML
Sbjct: 532 DIDV--DTKGGKTIGYVYVGETLA-----GVFNLSDACRSGVAQAMKELKSLG-IKIAML 591

Query: 639 TGDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALA 698
           TGD+ ++A      +G  ++ V   L PEDK   +K++ RE  G   MVG+G+NDAPALA
Sbjct: 592 TGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKRE-EGPTAMVGDGLNDAPALA 651

Query: 699 AATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLA 758
            A +GI +    SA AT   +++L+ + I  +P  I  +++    + +NV +++T     
Sbjct: 652 TADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAI 695

Query: 759 SLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRALN 787
              +  G   +W  VL   G  LLV LNS+  L+
Sbjct: 712 LALAFAGHPLIWAAVLADVGTCLLVILNSMLLLS 695

BLAST of Cucsa.017480 vs. TAIR10
Match: AT2G19110.1 (AT2G19110.1 heavy metal atpase 4)

HSP 1 Score: 228.0 bits (580), Expect = 2.1e-59
Identity = 177/635 (27.87%), Postives = 308/635 (48.50%), Query Frame = 1

Query: 159 FIAVAFPLVGVSASL-DALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 218
           ++AVA    G+   L  A   I   +++I++L+ +   A++ M + +E   ++ +F ++ 
Sbjct: 122 WLAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISD 181

Query: 219 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVG 278
             E   + ++   ++ L    P  A++ +  ++            V V +VKV + + V 
Sbjct: 182 WLETRASYKATSVMQSLMSLAPQKAIIAETGEE------------VEVDEVKVDTVVAVK 241

Query: 279 AGESVPVDCEVYQGCATITVEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEES 338
           AGE++P+D  V  G   +  + LTGE  P+  +    V  G  NL+G I VK T    + 
Sbjct: 242 AGETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDC 301

Query: 339 TLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 398
            ++++ +L EEAQ +K K QR +D+  + Y+  ++++S  VA++ P++ K         +
Sbjct: 302 VVAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIV-PVIMK-----VHNLK 361

Query: 399 GSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 458
              + AL ++V+  PC L  + P+A   A++  A  G+L+K    LD ++    VAFDKT
Sbjct: 362 HWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKT 421

Query: 459 GTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 518
           GT+T G  +                  F S       +  L   +++E  ++HP+   +V
Sbjct: 422 GTITRGEFIV---------------IDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIV 481

Query: 519 DH--SVGKDLPSISVESTEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSEN 578
           D+  SV  +     VE  + FPG G+   + G    IG K   +  G        C +  
Sbjct: 482 DYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAG--------CSTVP 541

Query: 579 ESRMIKDAVRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMML 638
           E       +   T G + V      ++     +L D  R GV  A+AEL+ LG ++  ML
Sbjct: 542 E-------IEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLG-IKTAML 601

Query: 639 TGDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALA 698
           TGD+ ++A      +G  ++ V+  L PEDK   ++E  +E  G   MVG+G+NDAPALA
Sbjct: 602 TGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE--GPTAMVGDGVNDAPALA 661

Query: 699 AATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLA 758
            A +GI +    SA AT   +++L+ + I  +P  +  +R+    + +NV L++  IL A
Sbjct: 662 TADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSI--ILKA 703

Query: 759 SLPSV--LGFLPLWLTVLLHEGGTLLVCLNSVRAL 786
            + ++   G   +W  VL+  G  LLV  NS+  L
Sbjct: 722 GILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLL 703

HSP 2 Score: 38.1 bits (87), Expect = 3.1e-02
Identity = 13/19 (68.42%), Postives = 13/19 (68.42%), Query Frame = 1

Query: 66   AAEASGHRHGHHHGHHHHH 85
            A E   HRH HHH HHHHH
Sbjct: 1151 AKELCSHRHHHHHHHHHHH 1169

BLAST of Cucsa.017480 vs. TAIR10
Match: AT5G21930.1 (AT5G21930.1 P-type ATPase of Arabidopsis 2)

HSP 1 Score: 209.9 bits (533), Expect = 5.9e-54
Identity = 179/586 (30.55%), Postives = 279/586 (47.61%), Query Frame = 1

Query: 208 LLLVMFNMAHIAEEYFTKQSMIDVKELKES-HPDFALVLDVNDDNLPDTSDLEHR----R 267
           +LL    +    EE    Q+  D+ EL         LV+  +D+N P  S L        
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 268 VPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVRPLQIKVGERVPGGARNL 327
           V V D++VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 328 DGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAV---- 387
           DG + +KA+ T   ST+S+IV++ E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFW 465

Query: 388 ALIGPLLFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSC 447
             +G  +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  
Sbjct: 466 YYVGSHIF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLG 525

Query: 448 ARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCI 507
           A++G L++GG VL+ +AS   VA DKTGTLT G  V   +  + G++             
Sbjct: 526 AKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE------------- 585

Query: 508 PSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLHGIKS 567
              E+E L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G   
Sbjct: 586 ---EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--- 645

Query: 568 GIGGKLRKASLGSVDFITS-FCKSENESRMIK----------DAVRASTYGSEFVHAALS 627
                 R  ++GS+++++  F K  + S M+K          +    S Y    V+    
Sbjct: 646 ------RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGRE 705

Query: 628 VDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGI--NEVYY 687
            +  +  I + D  R      +A LQ  G ++ ++L+GD + +   VA  VGI      Y
Sbjct: 706 GEGIIGAIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNY 765

Query: 688 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATA-VADVL 747
           SL PE K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+
Sbjct: 766 SLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVI 825

Query: 748 LLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFL 762
           L+++ +S V   ++ ++ T S + QN+  A+   ++ S+P   G L
Sbjct: 826 LVRNKLSHVVDALSLAQATMSKVYQNLAWAIAYNVI-SIPIAAGVL 840

BLAST of Cucsa.017480 vs. TAIR10
Match: AT4G33520.2 (AT4G33520.2 P-type ATP-ase 1)

HSP 1 Score: 189.5 bits (480), Expect = 8.2e-48
Identity = 187/675 (27.70%), Postives = 318/675 (47.11%), Query Frame = 1

Query: 173 LDALTDISGGKVNIHVLMALAAFASIFMGNPL-------------EGGLLLVMFNMAHIA 232
           LD +  +  G  N++ L+ L A +S  + +               E  +L+    +    
Sbjct: 305 LDGIKSLLKGSPNMNTLVGLGALSSFSVSSLAAMIPKLGWKTFFEEPVMLIAFVLLGRNL 364

Query: 233 EEYFTKQSMIDVKELKESHPDFA-LVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGA 292
           E+    ++  D+  L    P  A L+LD +  N    S +E   VP   + VG  +++  
Sbjct: 365 EQRAKIKATSDMTGLLSVLPSKARLLLDGDLQN----STVE---VPCNSLSVGDLVVILP 424

Query: 293 GESVPVDCEVYQGCATITVEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEEST 352
           G+ VP D  V  G +TI     TGE  P+  + G +V  G+ NL+G + V+  ++  E+ 
Sbjct: 425 GDRVPADGVVKSGRSTIDESSFTGEPLPVTKESGSQVAAGSINLNGTLTVEVHRSGGETA 484

Query: 353 LSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRG 412
           +  I++L EEAQ  +  +Q+ +D+    ++  V+ LS A       LF    + +    G
Sbjct: 485 VGDIIRLVEEAQSREAPVQQLVDKVAGRFTYGVMALSAATFTFWN-LFGAHVLPSALHNG 544

Query: 413 S-----VYRALGLMVAASPCALA-AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVA 472
           S     +  +  ++V A PCAL  A P A  +  S  AR+G+LL+GG +L+  +   TV 
Sbjct: 545 SPMSLALQLSCSVLVVACPCALGLATPTAMLVGTSLGARRGLLLRGGDILEKFSLVDTVV 604

Query: 473 FDKTGTLTTG-GLVFKAIEPIY-GHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHP 532
           FDKTGTLT G  +V + I P    H +    S          E E L +AAA+E  TTHP
Sbjct: 605 FDKTGTLTKGHPVVTEVIIPENPRHNLNDTWS----------EVEVLMLAAAVESNTTHP 664

Query: 533 IGRAVVDHSVGKDLPSISVESTEYF--PGRGLIATLHGIKSGIGGKLRKASLGSVDFITS 592
           +G+A+V  +  ++  ++  E   +   PG G +A ++          ++ ++G+++++  
Sbjct: 665 VGKAIVKAARARNCQTMKAEDGTFTEEPGSGAVAIVNN---------KRVTVGTLEWVKR 724

Query: 593 FCKSENESRMIKDAVRASTYGSEFVHAALSVDQKV-TLIHLEDQPRPGVIDAIAELQHLG 652
              + N       A+      ++ V   + VD  +  +I  ED+ R      +  L   G
Sbjct: 725 HGATGNSLL----ALEEHEINNQSV-VYIGVDNTLAAVIRFEDKVREDAAQVVENLTRQG 784

Query: 653 KLRVMMLTGDHDSSAWKVANAVGIN--EVYYSLKPEDKLAHVKEISREMGGGLIMVGEGI 712
            + V ML+GD  ++A  VA+ VGIN   V   +KP +K   + E+ +     + MVG+GI
Sbjct: 785 -IDVYMLSGDKRNAANYVASVVGINHERVIAGVKPAEKKNFINELQKNK-KIVAMVGDGI 844

Query: 713 NDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLA 772
           NDA ALA++ VG+ +   A A A+ V+ V+L+ + ++ +   +  SRQT   +KQN+  A
Sbjct: 845 NDAAALASSNVGVAMGGGAGA-ASEVSPVVLMGNRLTQLLDAMELSRQTMKTVKQNLWWA 904

Query: 773 LTSILLASLPSVLG-FLPLWLTVLLHEGGTLLVCLNSVRALNHPSWSWKQDLQNLIHDAR 820
                +  +P   G  LPL  T+L       L+ ++S+  + +        L+      R
Sbjct: 905 F-GYNIVGIPIAAGVLLPLTGTMLTPSMAGALMGVSSLGVMTNSLL-----LRYRFFSNR 938

BLAST of Cucsa.017480 vs. NCBI nr
Match: gi|449468396|ref|XP_004151907.1| (PREDICTED: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis sativus])

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 823/823 (100.00%), Postives = 823/823 (100.00%), Query Frame = 1

Query: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60
           MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120
           GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 824
           SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS
Sbjct: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 823

BLAST of Cucsa.017480 vs. NCBI nr
Match: gi|700208251|gb|KGN63370.1| (hypothetical protein Csa_2G433360 [Cucumis sativus])

HSP 1 Score: 1622.1 bits (4199), Expect = 0.0e+00
Identity = 817/823 (99.27%), Postives = 817/823 (99.27%), Query Frame = 1

Query: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60
           MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120
           GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG     L VLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG-----LKVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 824
           SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS
Sbjct: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 818

BLAST of Cucsa.017480 vs. NCBI nr
Match: gi|659125618|ref|XP_008462778.1| (PREDICTED: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis melo])

HSP 1 Score: 1609.3 bits (4166), Expect = 0.0e+00
Identity = 807/823 (98.06%), Postives = 811/823 (98.54%), Query Frame = 1

Query: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60
           MDTLFVPITFSELPHPLLT NTNA SQ R+GPVSRFSPLSSLIHLNRFP  FKKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60

Query: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120
           GHFRCAAEAS HR GHHHGHHHHHHGCQHHCSGDSD VELTG QKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDV+VGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEV+PLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 824
           SVRALNHPSWSWKQD QNLIH+ARSKLNTTPE SSGTIQTATS
Sbjct: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 823

BLAST of Cucsa.017480 vs. NCBI nr
Match: gi|657998222|ref|XP_008391510.1| (PREDICTED: LOW QUALITY PROTEIN: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Malus domestica])

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 600/794 (75.57%), Postives = 684/794 (86.15%), Query Frame = 1

Query: 32  PVSRFSPLSSLIHLNRFPTRFKKLSYPRFGHFRCAAEAS-GHRHGHHHGHHHHHHGCQHH 91
           P+ RF  L  L      P R +  + PR     C  +    H H HHH H HHHH   HH
Sbjct: 49  PIFRFKSLPPLP-----PPRLRNSNTPRCTSGACDRDHDHDHDHHHHHNHDHHHHHHHHH 108

Query: 92  CSGDSDGVELTGAQKAFVRFAEAIRWTDLANYLREHLHMCCGSAALFVTAAAFPYLVPKP 151
                D  ELTG+QK FV FA+A+RWTDLA++LREHL +C  SAAL + AAA PYL+PKP
Sbjct: 109 ----HDSAELTGSQKQFVAFAKAVRWTDLADFLREHLQLCFCSAALLLAAAACPYLMPKP 168

Query: 152 AVKPLQNVFIAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLL 211
           AVKP+QN FI VAFPLVGVSA+LDALTD+SGGKVNIHVLMALAAFAS+FMGN LEGGLLL
Sbjct: 169 AVKPMQNAFILVAFPLVGVSAALDALTDLSGGKVNIHVLMALAAFASVFMGNALEGGLLL 228

Query: 212 VMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKV 271
            MFN+AHIAEEYFT +SMIDVKELKE++PDFALVLD+ND  LP+TS+LE+++VPV D++V
Sbjct: 229 AMFNLAHIAEEYFTSRSMIDVKELKENYPDFALVLDINDGQLPNTSELEYKQVPVHDLQV 288

Query: 272 GSYILVGAGESVPVDCEVYQGCATITVEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKA 331
           GSYI VGAGESVPVDCEV+QG ATIT+EHLTGE++PL+ KVG+RVPGGARNLDGRII+KA
Sbjct: 289 GSYIFVGAGESVPVDCEVFQGTATITIEHLTGEIKPLETKVGDRVPGGARNLDGRIILKA 348

Query: 332 TKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPF 391
           TKTW+ESTLSRIVQLTEEAQLNKPKLQRWLD+FGE YSKVVVVLS A+AL+GP LFKWPF
Sbjct: 349 TKTWKESTLSRIVQLTEEAQLNKPKLQRWLDQFGEQYSKVVVVLSAAIALLGPFLFKWPF 408

Query: 392 ICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGGHVLDAMASCHT 451
           I T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGILLKGGHVLDA+ASCHT
Sbjct: 409 IGTSACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKGGHVLDALASCHT 468

Query: 452 VAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHP 511
           +AFDKTGTLTTGGL FKAIEPIYGH++    S F+SCC PSCEK+ALAVAAAMEKGTTHP
Sbjct: 469 IAFDKTGTLTTGGLAFKAIEPIYGHRMRNSISDFSSCCFPSCEKQALAVAAAMEKGTTHP 528

Query: 512 IGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLHGIKSGI-GGKLRKASLGSVDFITSF 571
           IGRAVVDHS GKDLPS+S+ES EYFPGRGLIATL+GI+ G  GG+L KASLGSVDFITS 
Sbjct: 529 IGRAVVDHSEGKDLPSVSIESFEYFPGRGLIATLNGIELGTEGGQLLKASLGSVDFITSL 588

Query: 572 CKSENESRMIKDAVRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKL 631
           C+S++ S  IK+AV AS+YG+EFV AALSV++KVTLIHLED+PRPGV D I EL+   KL
Sbjct: 589 CRSKDASEKIKEAVSASSYGTEFVRAALSVNEKVTLIHLEDRPRPGVSDVIEELKBQAKL 648

Query: 632 RVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAP 691
           RVMMLTGDHDSSAW+VAN+VGINEVY SLKPEDKL+HVK++SR+ GGGLIMVGEGINDAP
Sbjct: 649 RVMMLTGDHDSSAWRVANSVGINEVYCSLKPEDKLSHVKDVSRDTGGGLIMVGEGINDAP 708

Query: 692 ALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSI 751
           ALAAATVGIVLA RASATA AVADVLLL+D+IS VPFCIAKSRQTT+L+KQ+V LAL+ I
Sbjct: 709 ALAAATVGIVLAQRASATAIAVADVLLLRDNISAVPFCIAKSRQTTALVKQSVALALSCI 768

Query: 752 LLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRALNHPSWSWKQDLQNLIHDARSKLNT 811
           +LASLPSVLGFLPLWLTVLLHEGGTLLVCLNS+RALNHP+WSW+QDL +L+++ +SKL +
Sbjct: 769 ILASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNHPTWSWRQDLLHLVNELKSKLIS 828

Query: 812 --TPENSSGTIQTA 822
             T ++S  TIQ A
Sbjct: 829 PRTLQSSGNTIQAA 833

BLAST of Cucsa.017480 vs. NCBI nr
Match: gi|1009145303|ref|XP_015890260.1| (PREDICTED: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Ziziphus jujuba])

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 617/846 (72.93%), Postives = 705/846 (83.33%), Query Frame = 1

Query: 1   MDTLFVPITFSE----LPHPLLTCNTNAASQSRVGPVSRF---SPLSSLIHLNRFPTRFK 60
           M+TL  P++ S+    LP    TC  N+       P + F   +  SS I    FP+R  
Sbjct: 1   METLPYPLSLSKYSSLLPSRSATCRVNSPR-----PFTLFYNQALRSSSIFFPTFPSR-- 60

Query: 61  KLSYPR-FGHFRCAAEASGHRHGHHHG--HHH------------HHHGCQHHCSGDSDGV 120
               PR F H RCAA A+ H H HHH   HHH            HHH   H C    D  
Sbjct: 61  ----PRNFLHLRCAAHAADHHHHHHHDNTHHHDHHDGDHNHRDNHHHHHHHRCG---DCA 120

Query: 121 ELTGAQKAFVRFAEAIRWTDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNV 180
           ELTG Q+A ++FA A++WTDLA++LRE+LH CC S +LF+ AAA PYL+P+P VKPLQN 
Sbjct: 121 ELTGPQQALLKFATAVKWTDLADFLRENLHFCCFSTSLFLAAAACPYLLPRPTVKPLQNA 180

Query: 181 FIAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHI 240
           FI++AFPLVGVSASLDAL DISGGKVNIHVLMALAAFAS+ MGN LEGGLLL MFN+AHI
Sbjct: 181 FISIAFPLVGVSASLDALIDISGGKVNIHVLMALAAFASVVMGNGLEGGLLLAMFNLAHI 240

Query: 241 AEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGA 300
           AEEYFT +SMIDVKELKE++PDFALVLD+N+D LP+T DL ++RVPV DV+VGSYILVGA
Sbjct: 241 AEEYFTSRSMIDVKELKENYPDFALVLDINEDELPNTFDLAYKRVPVHDVEVGSYILVGA 300

Query: 301 GESVPVDCEVYQGCATITVEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEEST 360
           GESVPVDCEV+QG ATIT+EHLTGEV+PL+IKVG+R+PGGARNLDGR+IVKATKTW+EST
Sbjct: 301 GESVPVDCEVFQGSATITIEHLTGEVKPLEIKVGDRIPGGARNLDGRMIVKATKTWKEST 360

Query: 361 LSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRG 420
           LSRIVQLTEEA LNKPKLQRWLD+FGE YSKVVVVLS+AVALIGP LFKWPF+ T   RG
Sbjct: 361 LSRIVQLTEEAHLNKPKLQRWLDQFGERYSKVVVVLSLAVALIGPFLFKWPFVGTAACRG 420

Query: 421 SVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGT 480
           SVYRALGLMVAASPCALA APLAYA AISSCARKGILLKGGHVLDA+ASC T+AFDKTGT
Sbjct: 421 SVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKGGHVLDALASCRTIAFDKTGT 480

Query: 481 LTTGGLVFKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDH 540
           LTTGGL+FKAIEPIYGH +  + +  +SCC+PSCEKEALAVAAAMEKGTTHPIGRAVVDH
Sbjct: 481 LTTGGLMFKAIEPIYGHHVRNNSTGLSSCCVPSCEKEALAVAAAMEKGTTHPIGRAVVDH 540

Query: 541 SVGKDLPSISVESTEYFPGRGLIATLHGIKSGIGG-KLRKASLGSVDFITSFCKSENESR 600
           SVGKDLPS+SVES EYFPGRGL+ATL+  +S  GG KL KASLGSVDFITS CKSE ES 
Sbjct: 541 SVGKDLPSVSVESFEYFPGRGLVATLNSFESETGGSKLFKASLGSVDFITSLCKSE-ESE 600

Query: 601 MIKDAVRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGD 660
            IK+AV AS+YG EFV AALSV++KVTLIHLED+PRPGV+D IAELQH GKLR+MMLTGD
Sbjct: 601 KIKEAVNASSYGGEFVRAALSVNKKVTLIHLEDRPRPGVLDVIAELQHHGKLRIMMLTGD 660

Query: 661 HDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVG 720
           H SSA++VANAVGINEVY SLKPEDKL+HVK+ISR+MGGGLIMVGEGINDAPALAAATVG
Sbjct: 661 HKSSAYRVANAVGINEVYCSLKPEDKLSHVKDISRDMGGGLIMVGEGINDAPALAAATVG 720

Query: 721 IVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSV 780
           IVLA RASATA AVADVLLL+++ISGVPFC+AKSRQTTSL+KQNV LALT I+LASLPSV
Sbjct: 721 IVLAQRASATAIAVADVLLLRNNISGVPFCVAKSRQTTSLVKQNVALALTCIVLASLPSV 780

Query: 781 LGFLPLWLTVLLHEGGTLLVCLNSVRALNHPSWSWKQDLQNLIHDARSK--LNTTPENSS 822
           +GFLPLWLTVLLHEGGTLLVCLNS+RALN P+WSW QDL +L+   +S+  L++  + +S
Sbjct: 781 MGFLPLWLTVLLHEGGTLLVCLNSIRALNDPTWSWSQDLLHLLQKLKSRLILSSKHKAAS 831