Cucsa.017470 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.017470 vs. Swiss-Prot
Match: 4CL2_SOYBN (4-coumarate--CoA ligase 2 OS=Glycine max PE=2 SV=2)

HSP 1 Score: 756.5 bits (1952), Expect = 2.0e-217
Identity = 380/559 (67.98%), Postives = 458/559 (81.93%), Query Frame = 1

Query: 1   MISLAPLCD-PQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDR 60
           MI+LAP  D P+   N  S P  S    HVF+SKLPDIPI +HLPLH+Y FQ LSQ + R
Sbjct: 1   MITLAPSLDTPKTDQNQVSDPQTS----HVFKSKLPDIPISNHLPLHSYCFQNLSQFAHR 60

Query: 61  PCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLG 120
           PCLIVG   K+++Y++THL+S K AA  S LG+ +GDV+MILL NS +F+FSF+  SM+G
Sbjct: 61  PCLIVGPASKTFTYADTHLISSKIAAGLSNLGILKGDVVMILLQNSADFVFSFLAISMIG 120

Query: 121 AVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRE----SCGDVLTIVTIDAPPEN 180
           AVATTANP+YT  EI +Q   S AK ++T +  VDKLR       G+   +VT+D PPEN
Sbjct: 121 AVATTANPFYTAPEIFKQFTVSKAKLIITQAMYVDKLRNHDGAKLGEDFKVVTVDDPPEN 180

Query: 181 CLSFSMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGEN 240
           CL FS++ +A+E+DVP VEI  +DAVA+PFSSGTTGLPKGVILTHK++ +SVAQQVDGEN
Sbjct: 181 CLHFSVLSEANESDVPEVEIHPDDAVAMPFSSGTTGLPKGVILTHKSLTTSVAQQVDGEN 240

Query: 241 PNLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVA 300
           PNLYL   DV+LCVLP+FHIFSL+S++L ++R+G+A+LL++KFEI +LL L++RH+V+VA
Sbjct: 241 PNLYLTTEDVLLCVLPLFHIFSLNSVLLCALRAGSAVLLMQKFEIGTLLELIQRHRVSVA 300

Query: 301 TVVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEA 360
            VVPPLV++L KNP VADFDLSSIRLVLSGAAPL KELEEAL  R+PQA+ GQGYGMTEA
Sbjct: 301 MVVPPLVLALAKNPMVADFDLSSIRLVLSGAAPLGKELEEALRNRMPQAVLGQGYGMTEA 360

Query: 361 GPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMK 420
           GPVLSMC  FAK+ P  TKSGSCG VVRN+ELKVVDP TG SL YNQPGEIC+RG Q+MK
Sbjct: 361 GPVLSMCLGFAKQ-PFQTKSGSCGTVVRNAELKVVDPETGRSLGYNQPGEICIRGQQIMK 420

Query: 421 GYLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLV 480
           GYLND  +T+ T+D EGWLHTGD+G+VDD++EIFIVDRVKE+IK+KGFQV PAELE LLV
Sbjct: 421 GYLNDEAATASTIDSEGWLHTGDVGYVDDDDEIFIVDRVKELIKYKGFQVPPAELEGLLV 480

Query: 481 THTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVK 540
           +H SI DAAVVPQ D  AGEVPVAF+V S   +L+EE+VKEFIAKQVVFYKRLHKVYFV 
Sbjct: 481 SHPSIADAAVVPQKDVAAGEVPVAFVVRSNGFDLTEEAVKEFIAKQVVFYKRLHKVYFVH 540

Query: 541 TIPKSPSGKILRKELKAKL 555
            IPKSPSGKILRK+L+AKL
Sbjct: 541 AIPKSPSGKILRKDLRAKL 554

BLAST of Cucsa.017470 vs. Swiss-Prot
Match: 4CL3_ARATH (4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana GN=4CL3 PE=1 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 5.9e-217
Identity = 375/549 (68.31%), Postives = 462/549 (84.15%), Query Frame = 1

Query: 10  PQQPNNVSSSPPPSTPPT-HVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRPCLIVGSTG 69
           P   + VS    P +PPT  +FRSKLPDI IP+HLPLHTY F+KLS VSD+PCLIVGSTG
Sbjct: 14  PTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTG 73

Query: 70  KSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATTANPY 129
           KSY+Y ETHL+ R+ A+   KLG+++GDVIMILL NS EF+FSFMG+SM+GAV+TTANP+
Sbjct: 74  KSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPF 133

Query: 130 YTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAP-PENCLSFSMVYDADE 189
           YT  E+ +QLK+SGAK ++T+S+ VDKL+ + G+ LT++T D P PENCL FS +   DE
Sbjct: 134 YTSQELYKQLKSSGAKLIITHSQYVDKLK-NLGENLTLITTDEPTPENCLPFSTLITDDE 193

Query: 190 NDV--PSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDV 249
            +    +V+I  +DA ALPFSSGTTGLPKGV+LTHK++++SVAQQVDG+NPNLYLK NDV
Sbjct: 194 TNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDV 253

Query: 250 VLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPPLVVSL 309
           +LCVLP+FHI+SL+S++L S+RSGA +LL+ KFEI +LL L++RH+VT+A +VPPLV++L
Sbjct: 254 ILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIAL 313

Query: 310 VKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAF 369
            KNP V  +DLSS+R VLSGAAPL KEL+++L  RLPQAI GQGYGMTEAGPVLSM   F
Sbjct: 314 AKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGF 373

Query: 370 AKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLNDPVSTS 429
           AKEP +PTKSGSCG VVRN+ELKVV   T  SL YNQPGEIC+RG Q+MK YLNDP +TS
Sbjct: 374 AKEP-IPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATS 433

Query: 430 LTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAV 489
            T+D EGWLHTGDIG+VD+++EIFIVDR+KE+IKFKGFQV PAELE+LL+ H SI DAAV
Sbjct: 434 ATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAV 493

Query: 490 VPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKI 549
           VPQND+VAGEVPVAF+V S  N+++EE VKE++AKQVVFYKRLHKV+FV +IPKSPSGKI
Sbjct: 494 VPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKI 553

Query: 550 LRKELKAKL 555
           LRK+LKAKL
Sbjct: 554 LRKDLKAKL 560

BLAST of Cucsa.017470 vs. Swiss-Prot
Match: 4CL2_ORYSJ (Probable 4-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica GN=4CL2 PE=2 SV=2)

HSP 1 Score: 704.1 bits (1816), Expect = 1.2e-201
Identity = 360/566 (63.60%), Postives = 440/566 (77.74%), Query Frame = 1

Query: 5   APLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRPCLIV 64
           AP   PQ    V  +PP +     VFRSKLPDI IP HLPLH Y F + +++ D PCLI 
Sbjct: 6   APEAQPQVAAAVDEAPPEAVT---VFRSKLPDIDIPSHLPLHEYCFARAAELPDAPCLIA 65

Query: 65  GSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATT 124
            +TG++Y+++ET LL R+AAA   +LGV  GD +M+LL N  EF  +F  +S LGAV T 
Sbjct: 66  AATGRTYTFAETRLLCRRAAAALHRLGVGHGDRVMVLLQNCVEFAVAFFAASFLGAVTTA 125

Query: 125 ANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRE--------SC---GDVLTIVTID--- 184
           ANP+ T  EI +Q KASG K ++T S  VDKLR+        +C    D LT++TID   
Sbjct: 126 ANPFCTPQEIHKQFKASGVKLILTQSVYVDKLRQHEAFPRIDACTVGDDTLTVITIDDDE 185

Query: 185 APPENCLSF-SMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQ 244
           A PE CL F  ++ DADE  VP V I  +D VALPFSSGTTGLPKGV+LTH+++VS VAQ
Sbjct: 186 ATPEGCLPFWDLIADADEGSVPEVAISPDDPVALPFSSGTTGLPKGVVLTHRSVVSGVAQ 245

Query: 245 QVDGENPNLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVER 304
           QVDGENPNL++   DV LCVLP+FHIFSL+S++L ++R+GAA+ L+ +FE+ ++L  +ER
Sbjct: 246 QVDGENPNLHMGAGDVALCVLPLFHIFSLNSVLLCAVRAGAAVALMPRFEMGAMLGAIER 305

Query: 305 HKVTVATVVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQG 364
            +VTVA VVPPLV++L KNP V   DLSSIR+VLSGAAPL KELE+AL  RLPQAIFGQG
Sbjct: 306 WRVTVAAVVPPLVLALAKNPFVERHDLSSIRIVLSGAAPLGKELEDALRARLPQAIFGQG 365

Query: 365 YGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVR 424
           YGMTEAGPVLSMC AFAKEP  P KSGSCG VVRN+ELKVVDP TG SL  N PGEIC+R
Sbjct: 366 YGMTEAGPVLSMCPAFAKEPT-PAKSGSCGTVVRNAELKVVDPDTGFSLGRNLPGEICIR 425

Query: 425 GPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAE 484
           GPQ+MKGYLNDP +T+ T+DVEGWLHTGDIG+VDD++E+FIVDRVKE+IKFKGFQV PAE
Sbjct: 426 GPQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAE 485

Query: 485 LEALLVTHTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLH 544
           LE+LL+ H SI DAAVVPQ DDVAGEVPVAF+V +  ++++EES+KEFI+KQVVFYKRLH
Sbjct: 486 LESLLIAHPSIADAAVVPQKDDVAGEVPVAFVVRAADSDITEESIKEFISKQVVFYKRLH 545

Query: 545 KVYFVKTIPKSPSGKILRKELKAKLS 556
           KV+F+  IPKS SGKILR+EL+AKL+
Sbjct: 546 KVHFIHAIPKSASGKILRRELRAKLA 567

BLAST of Cucsa.017470 vs. Swiss-Prot
Match: 4CL_PINTA (4-coumarate--CoA ligase OS=Pinus taeda GN=4CL PE=2 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 4.9e-195
Identity = 326/529 (61.63%), Postives = 434/529 (82.04%), Query Frame = 1

Query: 28  HVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRPCLIVGSTGKSYSYSETHLLSRKAAATF 87
           H++RSKLPDI I DHLPLH+Y F+++++ +DRPCLI G+T ++Y +SE  L+SRK AA  
Sbjct: 10  HLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGL 69

Query: 88  SKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATTANPYYTGAEISRQLKASGAKFVV 147
           +KLG+++G V+M+LL N  EF F FMG+S+ GA+ TTANP+Y   EI++Q KA+GA+ +V
Sbjct: 70  AKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIV 129

Query: 148 TYSRCVDKLRE-SCGDVLTIVTIDAPPENCLSFSMVYDADENDVPSVEIDTNDAVALPFS 207
           T +  V+KL +    DVL I   DAP E C   S++ +ADE   P+V+I  +D VALP+S
Sbjct: 130 TLAAYVEKLADLQSHDVLVITIDDAPKEGCQHISVLTEADETQCPAVKIHPDDVVALPYS 189

Query: 208 SGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDVVLCVLPMFHIFSLSSIVLISM 267
           SGTTGLPKGV+LTHK +VSSVAQQVDGENPNLY   +DV+LCVLP+FHI+SL+S++L ++
Sbjct: 190 SGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSDDVILCVLPLFHIYSLNSVLLCAL 249

Query: 268 RSGAALLLIEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFDLSSIRLVLSGA 327
           R+GAA L+++KF + + L L++++KVTVA +VPP+V+ + K+P V+ +D+SS+R+++SGA
Sbjct: 250 RAGAATLIMQKFNLTTCLELIQKYKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGA 309

Query: 328 APLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSE 387
           APL KELE+AL ER P+AIFGQGYGMTEAGPVL+M  AFAK  P P KSGSCG VVRN++
Sbjct: 310 APLGKELEDALRERFPKAIFGQGYGMTEAGPVLAMNLAFAKN-PFPVKSGSCGTVVRNAQ 369

Query: 388 LKVVDPITGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGFVDDEE 447
           +K++D  TG SL +NQ GEIC+RGP++MKGY+NDP ST+ T+D EGWLHTGD+ ++DD+E
Sbjct: 370 IKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDE 429

Query: 448 EIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFIVPSTH 507
           EIFIVDRVKEIIK+KGFQVAPAELEALLV H SI DAAVVPQ  + AGEVPVAF+V S  
Sbjct: 430 EIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFVVKS-- 489

Query: 508 NELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKILRKELKAKLS 556
           +E+SE+ +KEF+AKQV+FYK++H+VYFV  IPKSPSGKILRK+L+++L+
Sbjct: 490 SEISEQEIKEFVAKQVIFYKKIHRVYFVDAIPKSPSGKILRKDLRSRLA 535

BLAST of Cucsa.017470 vs. Swiss-Prot
Match: 4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 3.6e-190
Identity = 315/528 (59.66%), Postives = 429/528 (81.25%), Query Frame = 1

Query: 29  VFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRPCLIVGSTGKSYSYSETHLLSRKAAATFS 88
           +FRSKLPDI IP HLPLHTY F+ +S+V D+ CLI G+TG++++YS+  LLSRK A+  +
Sbjct: 12  IFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLN 71

Query: 89  KLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATTANPYYTGAEISRQLKASGAKFVVT 148
           KLG+++GD IM+LL NSPE+ F+F+G+S  GA++T ANP++T AE+ +QLKAS AK ++T
Sbjct: 72  KLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKLIIT 131

Query: 149 YSRCVDKLRESCGDV-LTIVTIDAPPENCLSFSMVYDADENDVPSVEIDTNDAVALPFSS 208
            +  VDK+++   +  + I+ ID  P++CL FS + +ADE+++P V ID++D VALP+SS
Sbjct: 132 QACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVVALPYSS 191

Query: 209 GTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDVVLCVLPMFHIFSLSSIVLISMR 268
           GTTGLPKGV+LTHK +V+SVAQQVDG+NPNLY+   DV++C+LP+FHI+SL++++   +R
Sbjct: 192 GTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCGLR 251

Query: 269 SGAALLLIEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFDLSSIRLVLSGAA 328
           +G  +L+++KF+I   L L++++KVT+   VPP+V+++ K+P V  +DLSS+R V+SGAA
Sbjct: 252 AGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAA 311

Query: 329 PLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSEL 388
           PL KELE+A+  + P A  GQGYGMTEAGPVL+MC AFAKEP    KSG+CG VVRN+E+
Sbjct: 312 PLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEP-YEIKSGACGTVVRNAEM 371

Query: 389 KVVDPITGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGFVDDEEE 448
           K+VDP T ASL  NQ GEIC+RG Q+MKGYLNDP ST  T+D EGWLHTGDIGF+DD++E
Sbjct: 372 KIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDE 431

Query: 449 IFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFIVPSTHN 508
           +FIVDR+KEIIK+KGFQVAPAELEALL+TH +I DAAVVP  D+ AGEVPVAF+V +   
Sbjct: 432 LFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGF 491

Query: 509 ELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKILRKELKAKLS 556
             +EE +K+F++KQVVFYKR+ +V+FV  IPKSPSGKILRK+L+AK++
Sbjct: 492 TTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIA 538

BLAST of Cucsa.017470 vs. TrEMBL
Match: A0A0A0LQV1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G433350 PE=4 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 1.0e-276
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP 60
           MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP
Sbjct: 1   MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP 60

Query: 61  CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA 120
           CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA
Sbjct: 61  CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA 120

Query: 121 VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS 180
           VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS
Sbjct: 121 VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS 180

Query: 181 MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240
           MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL
Sbjct: 181 MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240

Query: 241 KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP 300
           KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP
Sbjct: 241 KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP 300

Query: 301 LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360
           LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS
Sbjct: 301 LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360

Query: 361 MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND 420
           MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND
Sbjct: 361 MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND 420

Query: 421 PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI 480
           PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI
Sbjct: 421 PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI 480

Query: 481 VDAAVVP 488
           VDAAVVP
Sbjct: 481 VDAAVVP 487

BLAST of Cucsa.017470 vs. TrEMBL
Match: B9S209_RICCO (AMP dependent CoA ligase, putative OS=Ricinus communis GN=RCOM_1325310 PE=4 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 5.4e-225
Identity = 394/568 (69.37%), Postives = 477/568 (83.98%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSS-----PPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQ 60
           MIS+A L  P+Q  + SSS     P  S+  TH+FRSKLPDIPI +HLPLHTY F+ L +
Sbjct: 1   MISIASLEPPKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLFK 60

Query: 61  VSDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGS 120
           +SD+PCLI GSTGK+YS++ETHL+S+K AA  S LG+K+GDVIMILL N PEF+FSFMG+
Sbjct: 61  LSDKPCLISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGA 120

Query: 121 SMLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRE-------SCGDVLTIVT 180
           SM+GAVATTANP+YT  EI +QL +S  K ++T S  VDKLR+         G+  T++T
Sbjct: 121 SMIGAVATTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVIT 180

Query: 181 IDAPPENCLSFSMVYDADENDVPSV-EIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSV 240
           ID PPENCL F+++ +A E ++P V   + +D VALPFSSGTTGLPKGVILTHK++++SV
Sbjct: 181 IDEPPENCLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITSV 240

Query: 241 AQQVDGENPNLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLV 300
           AQQVDGENPNLYLK +DVVLCVLP+FHI+SL+S++L S+R+GAA+LL++KF+I +LL L+
Sbjct: 241 AQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLELI 300

Query: 301 ERHKVTVATVVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFG 360
           ++HKV+VA VVPPLV++L KNP VA+FDLSSIR+VLSGAAPL KELE+AL  R+PQAI G
Sbjct: 301 QKHKVSVAAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILG 360

Query: 361 QGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEIC 420
           QGYGMTEAGPVLSMC  FAK+P  PTKSGSCG VVRN+ELKV+DP TG SL YNQPGEIC
Sbjct: 361 QGYGMTEAGPVLSMCLGFAKQP-FPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEIC 420

Query: 421 VRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAP 480
           +RGPQ+MKGYLNDP +T+ T+DVEGWLHTGDIG+VDD++EIFIVDRVKEIIKFKGFQV P
Sbjct: 421 IRGPQIMKGYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPP 480

Query: 481 AELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKR 540
           AELEALL+ H SI DAAVVPQ D+VAGEVPVAF+V S   ELSEE VKE+IAKQVVFYK+
Sbjct: 481 AELEALLLNHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELSEEDVKEYIAKQVVFYKK 540

Query: 541 LHKVYFVKTIPKSPSGKILRKELKAKLS 556
           LHKVYF+  IPKSPSGKILRK+LKAKL+
Sbjct: 541 LHKVYFIHAIPKSPSGKILRKDLKAKLA 567

BLAST of Cucsa.017470 vs. TrEMBL
Match: A0A0K1Z5X9_9ROSI (4-coumarate-CoA ligase (Fragment) OS=Citrus daoxianensis PE=2 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 3.5e-224
Identity = 388/559 (69.41%), Postives = 476/559 (85.15%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSSPPPSTP----PTHVFRSKLPDIPIPDHLPLHTYSFQKLSQV 60
           MIS+A    P+   ++SS+ PP+       TH+F+SKLPDIPI +HLPLHTY FQ   ++
Sbjct: 1   MISIATK-KPELSLDISSAAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQ--DRL 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSS 120
           SD PCLIVGSTGK+YSY+ETHL+ RK AA  S LG+K+GDVIMILL N  EF+FSFMG+S
Sbjct: 61  SDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGAS 120

Query: 121 MLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENC 180
           M+GAV TTANP+YT AEI +Q + SGAK ++T S+ VD+L ++  D  T++TIDAPPENC
Sbjct: 121 MIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLPKTDKD-FTVITIDAPPENC 180

Query: 181 LSFSMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240
           L F+++ +ADE+ +P V I  +D VALPFSSGTTGLPKGV+LTHK++++SVAQQVDGENP
Sbjct: 181 LHFTVLSEADEDQIPEVAIKPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENP 240

Query: 241 NLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVAT 300
           NLYL   DVVLCVLP+FHI+SL+S++L S+R+GA +LL++KFEI +LL L++RH+V+VA 
Sbjct: 241 NLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSVAA 300

Query: 301 VVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360
           VVPPLV++L KNP VAD+DLSSIR+VLSGAAPL KELE+AL  R+PQAI GQGYGMTEAG
Sbjct: 301 VVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRIPQAILGQGYGMTEAG 360

Query: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKG 420
           PVLSMC  FAK+P  PTKSGSCG VVRN+ELKV+DP TGASL +NQPGEIC+RGPQ+MKG
Sbjct: 361 PVLSMCLGFAKQP-FPTKSGSCGTVVRNAELKVIDPETGASLPHNQPGEICIRGPQIMKG 420

Query: 421 YLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480
           YLNDP +T+ T+DVEGWLHTGDIGFVDD++E+FIVDRVKEIIKFKGFQV PAE+EALL++
Sbjct: 421 YLNDPEATAATIDVEGWLHTGDIGFVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEALLLS 480

Query: 481 HTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKT 540
           H SI DAAVVPQ D+VAGEVPVAF+V S   EL+EE++KE+IAKQVVFYKRLHK+YFV  
Sbjct: 481 HPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKIYFVHA 540

Query: 541 IPKSPSGKILRKELKAKLS 556
           IPKSPSGKILRK+L+AKL+
Sbjct: 541 IPKSPSGKILRKDLRAKLA 554

BLAST of Cucsa.017470 vs. TrEMBL
Match: A0A0K1Z5Z2_CITJU (4-coumarate-CoA ligase (Fragment) OS=Citrus junos PE=2 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 7.7e-224
Identity = 387/559 (69.23%), Postives = 476/559 (85.15%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSSPPPSTP----PTHVFRSKLPDIPIPDHLPLHTYSFQKLSQV 60
           MIS+A    P+   ++SS  PP+       TH+F+SKLPDIPI +HLPLHTY FQ   ++
Sbjct: 1   MISIATK-KPELSLDISSPAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQ--DRL 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSS 120
           SD PCLIVGSTGK+YSY+ETHL+ RK AA  S LG+K+GD+IMILL N  EF+FSFMG+S
Sbjct: 61  SDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDMIMILLQNCAEFVFSFMGAS 120

Query: 121 MLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENC 180
           M+GAV TTANP+YT AEI +Q + SGAK ++T S+ VD+L ++  D  T++TIDAPPENC
Sbjct: 121 MIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLPKTDKD-FTVITIDAPPENC 180

Query: 181 LSFSMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240
           L F+++ +ADE+ +P V I+ +D VALPFSSGTTGLPKGV+LTHK++++SVAQQVDGENP
Sbjct: 181 LHFTVLSEADEDQIPEVAIEPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENP 240

Query: 241 NLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVAT 300
           NLYL   DVVLCVLP+FHI+SL+S++L S+R+GA +LL++KFEI +LL L++RH+V+VA 
Sbjct: 241 NLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSVAA 300

Query: 301 VVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360
           VVPPLV++L KNP VAD+DLSSIR+VLSGAAPL KELE+AL  R+PQAI GQGYGMTEAG
Sbjct: 301 VVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAG 360

Query: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKG 420
           PVLSMC  FAK+P  PTKSGSCG VVRN+ELKV+DP TGASL +NQPGEIC+RGPQ+MKG
Sbjct: 361 PVLSMCLGFAKQP-FPTKSGSCGTVVRNAELKVIDPETGASLPHNQPGEICIRGPQIMKG 420

Query: 421 YLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480
           YLNDP +T+ T+DVEGWLHTGDIGFVDD++E+FIVDRVKEIIKFKGFQV PAE+EALL++
Sbjct: 421 YLNDPEATAATIDVEGWLHTGDIGFVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEALLLS 480

Query: 481 HTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKT 540
           H SI DAAVVPQ D+VAGEVPVAF+V S   EL+EE++KE+IAKQVVFYKRLHK+YFV  
Sbjct: 481 HPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKIYFVHA 540

Query: 541 IPKSPSGKILRKELKAKLS 556
           IPKSPSGKILRK+L+AKL+
Sbjct: 541 IPKSPSGKILRKDLRAKLA 554

BLAST of Cucsa.017470 vs. TrEMBL
Match: A0A0K1Z5C4_CITRE (4-coumarate-CoA ligase (Fragment) OS=Citrus reticulata PE=2 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 5.0e-223
Identity = 386/559 (69.05%), Postives = 475/559 (84.97%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSSPPPSTP----PTHVFRSKLPDIPIPDHLPLHTYSFQKLSQV 60
           MIS+A    P+   ++SS+ PP+       TH+F+SKLPDI I +HLPLHTY FQ   ++
Sbjct: 1   MISIATK-KPELSLDISSAAPPAPSNEKIATHIFKSKLPDISISNHLPLHTYCFQ--DRL 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSS 120
           SD PCLIVGSTGK+YSY+ETHL+ RK AA  S LG+K+GDVIMILL N  EF+FSFMG+S
Sbjct: 61  SDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGAS 120

Query: 121 MLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENC 180
           M+GAV TTANP+YT AEI +Q + SGAK ++T S+ VD+L ++  D  T++TIDAPPENC
Sbjct: 121 MIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLPKTDKD-FTVITIDAPPENC 180

Query: 181 LSFSMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240
           L F+++ +ADE+ +P V I  +D VALPFSSGTTGLPKGV+LTHK++++SVAQQVDGENP
Sbjct: 181 LHFTVLSEADEDQIPEVAIKPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENP 240

Query: 241 NLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVAT 300
           NLYL   DVVLCVLP+FHI+SL+S++L S+R+GA +LL++KFEI +LL L++RH+V+VA 
Sbjct: 241 NLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSVAA 300

Query: 301 VVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360
           VVPPLV++L KNP VAD+DLSS+R+VLSGAAPL KELE+AL  R+PQAI GQGYGMTEAG
Sbjct: 301 VVPPLVLALAKNPMVADYDLSSVRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAG 360

Query: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKG 420
           PVLSMC  FAK+P  PTKSGSCG VVRN+ELKV+DP TGASL +NQPGEIC+RGPQ+MKG
Sbjct: 361 PVLSMCLGFAKQP-FPTKSGSCGTVVRNAELKVIDPETGASLPHNQPGEICIRGPQIMKG 420

Query: 421 YLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480
           YLNDP +T+ T+DVEGWLHTGDIGFVDD++E+FIVDRVKEIIKFKGFQV PAE+EALL++
Sbjct: 421 YLNDPEATAATIDVEGWLHTGDIGFVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEALLLS 480

Query: 481 HTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKT 540
           H SI DAAVVPQ D+VAGEVPVAF+V S   EL+EE++KE+IAKQVVFYKRLHK+YFV  
Sbjct: 481 HPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKIYFVHA 540

Query: 541 IPKSPSGKILRKELKAKLS 556
           IPKSPSGKILRK+L+AKL+
Sbjct: 541 IPKSPSGKILRKDLRAKLA 554

BLAST of Cucsa.017470 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 755.0 bits (1948), Expect = 3.3e-218
Identity = 375/549 (68.31%), Postives = 462/549 (84.15%), Query Frame = 1

Query: 10  PQQPNNVSSSPPPSTPPT-HVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRPCLIVGSTG 69
           P   + VS    P +PPT  +FRSKLPDI IP+HLPLHTY F+KLS VSD+PCLIVGSTG
Sbjct: 14  PTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTG 73

Query: 70  KSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATTANPY 129
           KSY+Y ETHL+ R+ A+   KLG+++GDVIMILL NS EF+FSFMG+SM+GAV+TTANP+
Sbjct: 74  KSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPF 133

Query: 130 YTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAP-PENCLSFSMVYDADE 189
           YT  E+ +QLK+SGAK ++T+S+ VDKL+ + G+ LT++T D P PENCL FS +   DE
Sbjct: 134 YTSQELYKQLKSSGAKLIITHSQYVDKLK-NLGENLTLITTDEPTPENCLPFSTLITDDE 193

Query: 190 NDV--PSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDV 249
            +    +V+I  +DA ALPFSSGTTGLPKGV+LTHK++++SVAQQVDG+NPNLYLK NDV
Sbjct: 194 TNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDV 253

Query: 250 VLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPPLVVSL 309
           +LCVLP+FHI+SL+S++L S+RSGA +LL+ KFEI +LL L++RH+VT+A +VPPLV++L
Sbjct: 254 ILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIAL 313

Query: 310 VKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAF 369
            KNP V  +DLSS+R VLSGAAPL KEL+++L  RLPQAI GQGYGMTEAGPVLSM   F
Sbjct: 314 AKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGF 373

Query: 370 AKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLNDPVSTS 429
           AKEP +PTKSGSCG VVRN+ELKVV   T  SL YNQPGEIC+RG Q+MK YLNDP +TS
Sbjct: 374 AKEP-IPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATS 433

Query: 430 LTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAV 489
            T+D EGWLHTGDIG+VD+++EIFIVDR+KE+IKFKGFQV PAELE+LL+ H SI DAAV
Sbjct: 434 ATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAV 493

Query: 490 VPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKI 549
           VPQND+VAGEVPVAF+V S  N+++EE VKE++AKQVVFYKRLHKV+FV +IPKSPSGKI
Sbjct: 494 VPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKI 553

Query: 550 LRKELKAKL 555
           LRK+LKAKL
Sbjct: 554 LRKDLKAKL 560

BLAST of Cucsa.017470 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 657.1 bits (1694), Expect = 9.4e-189
Identity = 320/531 (60.26%), Postives = 424/531 (79.85%), Query Frame = 1

Query: 29  VFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRPCLIVGSTGKSYSYSETHLLSRKAAATFS 88
           +FRS+LPDI IP+HLPLH Y F+ +S+ + +PCLI G TG+ Y+Y++ H+ SRK AA   
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 89  KLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATTANPYYTGAEISRQLKASGAKFVVT 148
            LGVK+ DV+MILL NSPE + +F+ +S +GA+ T+ANP++T AEIS+Q KAS AK +VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 149 YSRCVDKLRESCGDVLTIVTID--APPENCLSFSMVYDADENDVPSV--EIDTNDAVALP 208
            SR VDK++    D + IVT D  A PENCL FS +  ++E  V S+  +I   D VALP
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDVVALP 201

Query: 209 FSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDVVLCVLPMFHIFSLSSIVLI 268
           FSSGTTGLPKGV+LTHK +V+SVAQQVDGENPNLY  R+DV+LCVLPMFHI++L+SI+L 
Sbjct: 202 FSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLC 261

Query: 269 SMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFDLSSIRLVLS 328
           S+R GA +L++ KFEI  LL  ++R KVTVA VVPP+V+++ K+P+   +DLSS+R+V S
Sbjct: 262 SLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRMVKS 321

Query: 329 GAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRN 388
           GAAPL KELE+A+  + P A  GQGYGMTEAGPVL+M   FAKEP  P KSG+CG VVRN
Sbjct: 322 GAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEP-FPVKSGACGTVVRN 381

Query: 389 SELKVVDPITGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGFVDD 448
           +E+K++DP TG SL  N+PGEIC+RG Q+MKGYLNDP++T+ T+D +GWLHTGD+GF+DD
Sbjct: 382 AEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDD 441

Query: 449 EEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFIVPS 508
           ++E+FIVDR+KE+IK+KGFQVAPAELE+LL+ H  I D AVV   ++ AGEVPVAF+V S
Sbjct: 442 DDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRS 501

Query: 509 THNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKILRKELKAKLS 556
             + +SE+ +K+F++KQVVFYKR++KV+F  +IPK+PSGKILRK+L+A+L+
Sbjct: 502 KDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLA 551

BLAST of Cucsa.017470 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 644.8 bits (1662), Expect = 4.8e-185
Identity = 320/536 (59.70%), Postives = 418/536 (77.99%), Query Frame = 1

Query: 29  VFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRPCLIVGSTGKSYSYSETHLLSRKAAATFS 88
           +FRSKLPDI IP+HL LH Y FQ +S+ + +PCLI G TG  Y+YS+ H++SR+ AA F 
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 89  KLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATTANPYYTGAEISRQLKASGAKFVVT 148
           KLGV + DV+M+LL N PEF+ SF+ +S  GA AT ANP++T AEI++Q KAS  K ++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 149 YSRCVDKLRE-SCGDVLTIVTIDAP-----PENCLSFSMVYDADEND---VPSVEIDTND 208
            +R VDK++     D + IV ID       PE CL F+ +  +       + SVEI  +D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 209 AVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDVVLCVLPMFHIFSLS 268
            VALP+SSGTTGLPKGV+LTHK +V+SVAQQVDGENPNLY   +DV+LCVLPMFHI++L+
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 269 SIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFDLSSI 328
           SI+L  +R GAA+L++ KFEI  LL L++R KVTVA +VPP+V+++ K+ +   +DLSSI
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 329 RLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCG 388
           R+V SGAAPL KELE+A+  + P A  GQGYGMTEAGPVL+M   FAKEP  P KSG+CG
Sbjct: 324 RVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEP-FPVKSGACG 383

Query: 389 RVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDI 448
            VVRN+E+K+VDP TG SL+ NQPGEIC+RG Q+MKGYLN+P +T+ T+D +GWLHTGDI
Sbjct: 384 TVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDI 443

Query: 449 GFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVA 508
           G +DD++E+FIVDR+KE+IK+KGFQVAPAELEALL+ H  I D AVV   ++ AGEVPVA
Sbjct: 444 GLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVA 503

Query: 509 FIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKILRKELKAKLS 556
           F+V S  +ELSE+ VK+F++KQVVFYKR++KV+F ++IPK+PSGKILRK+L+AKL+
Sbjct: 504 FVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of Cucsa.017470 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 595.9 bits (1535), Expect = 2.6e-170
Identity = 301/544 (55.33%), Postives = 406/544 (74.63%), Query Frame = 1

Query: 26  PTH--VFRSKLPDIPIPDHLPLHTYSFQKLSQVSD----RPCLIVGSTGKSYSYSETHLL 85
           P+H  +FRSKLPDI IP+HLPL  Y FQ+ S   D      C+I G+TG+  +Y++    
Sbjct: 23  PSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTN 82

Query: 86  SRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATTANPYYTGAEISRQLK 145
            R+ AA   +LG++ GDV+M+LL NSPEF  SF+  + LGAV+TTANP+YT  EI++Q K
Sbjct: 83  MRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAK 142

Query: 146 ASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPEN---------CLSFSMVYDADENDV 205
           AS AK ++T    VDKL     D + IV +D   +N         C+SF+ +  ADE ++
Sbjct: 143 ASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETEL 202

Query: 206 PSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDVVLCVL 265
              +I   D VA+P+SSGTTGLPKGV++THK +V+S+AQ+VDGENPNL    NDV+LC L
Sbjct: 203 LKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFL 262

Query: 266 PMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPK 325
           PMFHI++L +++L +MR+GAALL++ +FE+  ++ L++R+KVTV  V PP+V++ +K+P+
Sbjct: 263 PMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSPE 322

Query: 326 VADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPP 385
              +DLSS+R++LSGAA L+KELE+A+  + P AIFGQGYGMTE+G V     AFAK P 
Sbjct: 323 TERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAKSL-AFAKNP- 382

Query: 386 MPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDV 445
             TKSG+CG V+RN+E+KVVD  TG SL  N+ GEICVRG Q+MKGYLNDP +T+ T+D 
Sbjct: 383 FKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDK 442

Query: 446 EGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQND 505
           +GWLHTGDIGFVDD++EIFIVDR+KE+IKFKG+QVAPAELEALL++H SI DAAVV   D
Sbjct: 443 DGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKD 502

Query: 506 DVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKILRKEL 555
           +VA EVPVAF+  S  ++L+E+ VK ++ KQVV YKR+  V+F++ IPK+ SGKILRK+L
Sbjct: 503 EVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDL 562

BLAST of Cucsa.017470 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 391.7 bits (1005), Expect = 7.4e-109
Identity = 220/537 (40.97%), Postives = 331/537 (61.64%), Query Frame = 1

Query: 29  VFRSKLPDIPIPD--HLPLHTYSFQKLSQVSDRPCLIVGSTGKSYSYSETHLLSRKAAAT 88
           ++RS  P + +P   +  L ++ F+  S    +  +    TG S ++S+      + A  
Sbjct: 11  IYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG 70

Query: 89  FSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGAVATTANPYYTGAEISRQLKASGAKFV 148
           F +LG+++ DV++I   NS +F   F+  + +G V TTANP YT  E+S+Q+K S  K +
Sbjct: 71  FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKII 130

Query: 149 VTYSRCVDKLRESCGDVLTIV------TIDAPP---ENCLSFSMVYDADE--NDVPSVEI 208
           ++ ++  DK++   G  L +V      T++ PP      LSF  V +  E  ++ P VEI
Sbjct: 131 ISVNQLFDKIK---GFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEI 190

Query: 209 DTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDVVLCVLPMFHI 268
             +D  AL +SSGTTG  KGV LTH N ++  A  +   + +L  + + V LC LPMFH+
Sbjct: 191 KQSDTAALLYSSGTTGTSKGVELTHGNFIA--ASLMVTMDQDLMGEYHGVFLCFLPMFHV 250

Query: 269 FSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFD 328
           F L+ I    ++ G AL+ + +FE+E +L+ +E+ +VT   VVPP+ ++L K   V  FD
Sbjct: 251 FGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFD 310

Query: 329 LSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTK- 388
           LSS++ + SGAAPL K+L E     +P  +  QGYGMTE       C   + E P   K 
Sbjct: 311 LSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTET------CGIVSVEDPRLGKR 370

Query: 389 -SGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGW 448
            SGS G +    E ++V   TG S   NQ GEI VRGP +MKGYLN+P +T  T+D + W
Sbjct: 371 NSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSW 430

Query: 449 LHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVA 508
           +HTGD+G+ +++  +++VDR+KE+IK+KGFQVAPAELE LLV+H  I+DA V+P  D+ A
Sbjct: 431 VHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEA 490

Query: 509 GEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKILRKEL 551
           GEVP+AF+V S ++ ++E+ +++FIAKQV  YKRL +V F+  +PKS +GKILR+EL
Sbjct: 491 GEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRREL 536

BLAST of Cucsa.017470 vs. NCBI nr
Match: gi|449468398|ref|XP_004151908.1| (PREDICTED: 4-coumarate--CoA ligase 2 [Cucumis sativus])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP 60
           MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP
Sbjct: 1   MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP 60

Query: 61  CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA 120
           CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA
Sbjct: 61  CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA 120

Query: 121 VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS 180
           VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS
Sbjct: 121 VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS 180

Query: 181 MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240
           MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL
Sbjct: 181 MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240

Query: 241 KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP 300
           KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP
Sbjct: 241 KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP 300

Query: 301 LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360
           LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS
Sbjct: 301 LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360

Query: 361 MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND 420
           MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND
Sbjct: 361 MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND 420

Query: 421 PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI 480
           PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI
Sbjct: 421 PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI 480

Query: 481 VDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKS 540
           VDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKS
Sbjct: 481 VDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKS 540

Query: 541 PSGKILRKELKAKLSIT 558
           PSGKILRKELKAKLSIT
Sbjct: 541 PSGKILRKELKAKLSIT 557

BLAST of Cucsa.017470 vs. NCBI nr
Match: gi|659125620|ref|XP_008462779.1| (PREDICTED: 4-coumarate--CoA ligase 2 [Cucumis melo])

HSP 1 Score: 1038.9 bits (2685), Expect = 3.3e-300
Identity = 528/559 (94.45%), Postives = 545/559 (97.50%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSS----SPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQV 60
           MISLAP+CDPQ PNNVSS    SPPPSTP THVFRSKLPDIPIPDHLPLH+Y FQKLSQV
Sbjct: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSS 120
           SDRPCLIVGSTGKSYSYSETHL SRKAAATFSKLGV+RGDVIMILLHNSPEFIFSFMGSS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENC 180
           MLGA+ATTANPYYTGAEIS+QLKASGAKFVVTYSRCVDKLRE CG+VLTIVT+D PPENC
Sbjct: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRE-CGEVLTIVTVDDPPENC 180

Query: 181 LSFSMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240
           LSFSMVYDA+ENDVP VEIDTNDAV+LPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP
Sbjct: 181 LSFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240

Query: 241 NLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVAT 300
           NLYLKRND+VLCVLPMFHIFSLSSIVLIS+RSGAALLL+EKFEIESLLRLVERHKVTVAT
Sbjct: 241 NLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVAT 300

Query: 301 VVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360
           VVPPLVVSLVKNPKVADF+LSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG
Sbjct: 301 VVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360

Query: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKG 420
           PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDP+TGASLTYNQPGEICVRGPQVMKG
Sbjct: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKG 420

Query: 421 YLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480
           YLNDPVSTSLTVDVEGWLHTGDIG+VDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT
Sbjct: 421 YLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480

Query: 481 HTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKT 540
           HTSIVDAAVVPQNDDVAGEVPVAF+VPST N L+EESVKEFIAKQVVFYKRLHKVYFVKT
Sbjct: 481 HTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKT 540

Query: 541 IPKSPSGKILRKELKAKLS 556
           IPKSPSGK LRKELKAKLS
Sbjct: 541 IPKSPSGKTLRKELKAKLS 558

BLAST of Cucsa.017470 vs. NCBI nr
Match: gi|700208250|gb|KGN63369.1| (hypothetical protein Csa_2G433350 [Cucumis sativus])

HSP 1 Score: 960.3 bits (2481), Expect = 1.5e-276
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP 60
           MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP
Sbjct: 1   MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP 60

Query: 61  CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA 120
           CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA
Sbjct: 61  CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA 120

Query: 121 VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS 180
           VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS
Sbjct: 121 VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS 180

Query: 181 MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240
           MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL
Sbjct: 181 MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240

Query: 241 KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP 300
           KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP
Sbjct: 241 KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP 300

Query: 301 LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360
           LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS
Sbjct: 301 LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360

Query: 361 MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND 420
           MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND
Sbjct: 361 MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND 420

Query: 421 PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI 480
           PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI
Sbjct: 421 PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI 480

Query: 481 VDAAVVP 488
           VDAAVVP
Sbjct: 481 VDAAVVP 487

BLAST of Cucsa.017470 vs. NCBI nr
Match: gi|255557999|ref|XP_002520028.1| (PREDICTED: 4-coumarate--CoA ligase 2 [Ricinus communis])

HSP 1 Score: 788.5 bits (2035), Expect = 7.7e-225
Identity = 394/568 (69.37%), Postives = 477/568 (83.98%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSS-----PPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQ 60
           MIS+A L  P+Q  + SSS     P  S+  TH+FRSKLPDIPI +HLPLHTY F+ L +
Sbjct: 1   MISIASLEPPKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLFK 60

Query: 61  VSDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGS 120
           +SD+PCLI GSTGK+YS++ETHL+S+K AA  S LG+K+GDVIMILL N PEF+FSFMG+
Sbjct: 61  LSDKPCLISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGA 120

Query: 121 SMLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRE-------SCGDVLTIVT 180
           SM+GAVATTANP+YT  EI +QL +S  K ++T S  VDKLR+         G+  T++T
Sbjct: 121 SMIGAVATTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVIT 180

Query: 181 IDAPPENCLSFSMVYDADENDVPSV-EIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSV 240
           ID PPENCL F+++ +A E ++P V   + +D VALPFSSGTTGLPKGVILTHK++++SV
Sbjct: 181 IDEPPENCLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITSV 240

Query: 241 AQQVDGENPNLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLV 300
           AQQVDGENPNLYLK +DVVLCVLP+FHI+SL+S++L S+R+GAA+LL++KF+I +LL L+
Sbjct: 241 AQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLELI 300

Query: 301 ERHKVTVATVVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFG 360
           ++HKV+VA VVPPLV++L KNP VA+FDLSSIR+VLSGAAPL KELE+AL  R+PQAI G
Sbjct: 301 QKHKVSVAAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILG 360

Query: 361 QGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEIC 420
           QGYGMTEAGPVLSMC  FAK+P  PTKSGSCG VVRN+ELKV+DP TG SL YNQPGEIC
Sbjct: 361 QGYGMTEAGPVLSMCLGFAKQP-FPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEIC 420

Query: 421 VRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAP 480
           +RGPQ+MKGYLNDP +T+ T+DVEGWLHTGDIG+VDD++EIFIVDRVKEIIKFKGFQV P
Sbjct: 421 IRGPQIMKGYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPP 480

Query: 481 AELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKR 540
           AELEALL+ H SI DAAVVPQ D+VAGEVPVAF+V S   ELSEE VKE+IAKQVVFYK+
Sbjct: 481 AELEALLLNHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELSEEDVKEYIAKQVVFYKK 540

Query: 541 LHKVYFVKTIPKSPSGKILRKELKAKLS 556
           LHKVYF+  IPKSPSGKILRK+LKAKL+
Sbjct: 541 LHKVYFIHAIPKSPSGKILRKDLKAKLA 567

BLAST of Cucsa.017470 vs. NCBI nr
Match: gi|916370561|gb|AKZ20933.1| (4-coumarate-CoA ligase, partial [Citrus daoxianensis])

HSP 1 Score: 785.8 bits (2028), Expect = 5.0e-224
Identity = 388/559 (69.41%), Postives = 476/559 (85.15%), Query Frame = 1

Query: 1   MISLAPLCDPQQPNNVSSSPPPSTP----PTHVFRSKLPDIPIPDHLPLHTYSFQKLSQV 60
           MIS+A    P+   ++SS+ PP+       TH+F+SKLPDIPI +HLPLHTY FQ   ++
Sbjct: 1   MISIATK-KPELSLDISSAAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQ--DRL 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSS 120
           SD PCLIVGSTGK+YSY+ETHL+ RK AA  S LG+K+GDVIMILL N  EF+FSFMG+S
Sbjct: 61  SDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGAS 120

Query: 121 MLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENC 180
           M+GAV TTANP+YT AEI +Q + SGAK ++T S+ VD+L ++  D  T++TIDAPPENC
Sbjct: 121 MIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLPKTDKD-FTVITIDAPPENC 180

Query: 181 LSFSMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240
           L F+++ +ADE+ +P V I  +D VALPFSSGTTGLPKGV+LTHK++++SVAQQVDGENP
Sbjct: 181 LHFTVLSEADEDQIPEVAIKPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENP 240

Query: 241 NLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVAT 300
           NLYL   DVVLCVLP+FHI+SL+S++L S+R+GA +LL++KFEI +LL L++RH+V+VA 
Sbjct: 241 NLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSVAA 300

Query: 301 VVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360
           VVPPLV++L KNP VAD+DLSSIR+VLSGAAPL KELE+AL  R+PQAI GQGYGMTEAG
Sbjct: 301 VVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRIPQAILGQGYGMTEAG 360

Query: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKG 420
           PVLSMC  FAK+P  PTKSGSCG VVRN+ELKV+DP TGASL +NQPGEIC+RGPQ+MKG
Sbjct: 361 PVLSMCLGFAKQP-FPTKSGSCGTVVRNAELKVIDPETGASLPHNQPGEICIRGPQIMKG 420

Query: 421 YLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480
           YLNDP +T+ T+DVEGWLHTGDIGFVDD++E+FIVDRVKEIIKFKGFQV PAE+EALL++
Sbjct: 421 YLNDPEATAATIDVEGWLHTGDIGFVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEALLLS 480

Query: 481 HTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKT 540
           H SI DAAVVPQ D+VAGEVPVAF+V S   EL+EE++KE+IAKQVVFYKRLHK+YFV  
Sbjct: 481 HPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKIYFVHA 540

Query: 541 IPKSPSGKILRKELKAKLS 556
           IPKSPSGKILRK+L+AKL+
Sbjct: 541 IPKSPSGKILRKDLRAKLA 554