Cucsa.012110 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.012110 vs. Swiss-Prot
Match: MACP1_ARATH (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 5.7e-194
Identity = 347/616 (56.33%), Postives = 442/616 (71.75%), Query Frame = 1

Query: 4   EIVEKALNSLGKGFDLTSDFRLKYCK------GQRSLVLLNQSQRRDLIVPGFGAVKDVS 63
           +++E A+ SLGKGFDLT+DFRLKYCK      G   LV+L+Q+Q R+L +PGFG  ++VS
Sbjct: 7   DVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVS 66

Query: 64  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 123
            DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FN+ FGFQSG WATDA N 
Sbjct: 67  ADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANV 126

Query: 124 KCLGLDGYFISLFNAHIDR-YPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIG 183
           K LGLD   ++LFN HI     LRL+D VR+AVPS+WDP  +ARFIE+YGTH+I G+S+G
Sbjct: 127 KSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVG 186

Query: 184 GRDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFP--------------PPIPQAF 243
           G+DVV+VRQDKSS L+   L++HL +LGDQLF G+C                 P  P+AF
Sbjct: 187 GQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 246

Query: 244 NLFDP-QLVAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPI 303
           N+FD  Q VAF NFS +++++GI VI +KRGG   A SH EWL TV + PDAI+F FIPI
Sbjct: 247 NVFDDKQTVAFNNFS-INSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 306

Query: 304 ISLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTS 363
            SLL+ +PG G LSHA++LYLRYKPP+ DLQYFLDF   + WAP+HNDLP G  PN++++
Sbjct: 307 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 366

Query: 364 -PSLQLNLMGPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNI 423
            P+L +N MGPKLYVNTT V ++  PVTGMR +LEG K NRLAIH+QHL  T   +   I
Sbjct: 367 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 426

Query: 424 TQQTYWHSSQHIAD-DRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNS--PAAFIVTG 483
           T +  W  S  I D DRY EPLN  KFSHVCT+P+KYDP W  T+ +  S    AFIVTG
Sbjct: 427 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 486

Query: 484 AQLLVVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSA 543
           AQ L VK+H SK+VLHL L Y++V + Y+VQ +W       S KSGIFS++S  LT  S 
Sbjct: 487 AQ-LEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSV 546

Query: 544 ALKE-KPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLN 593
                +  +  V++DSGVF GGPP+     ++VKFVDLS++ +GPQ SPGHW+V G RL 
Sbjct: 547 HHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLY 606

BLAST of Cucsa.012110 vs. Swiss-Prot
Match: MACP2_ARATH (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.8e-137
Identity = 274/604 (45.36%), Postives = 378/604 (62.58%), Query Frame = 1

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCKG----QRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A +  E A+ S+G G+DL  D RLKYCKG     R L +       ++++PG  ++ +VS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+R R++SDIL F QM+E FN++ S+ GKIPSG FN+MF F S CW  DA  T
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSS-CWQKDAAYT 126

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG FISL++  +D+  + L + V+ AVPSTWDP A+ARFI+ YGTHIIV + +GG
Sbjct: 127 KNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGG 186

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPIPQAFNLFDP-----QLVAF 241
           +DV+  +Q  SSKL+P +L+  L+E+ D+ F  A        +            Q + F
Sbjct: 187 KDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRF 246

Query: 242 GNFSSVSA---KDGIIVITSKRGGSPTAS-SHCEWLPTVHESPDAIHFQFIPIISLLECI 301
            + SS+ +   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL  +
Sbjct: 247 ADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGV 306

Query: 302 PGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNL 361
           PG GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +  SLQ + 
Sbjct: 307 PGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSF 366

Query: 362 MGPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLL--LENNITQQTYW 421
            GPKLYVNTT V    RP+TGMRLYLEG +SNRLAIH+QHL + P +  LE+++ +    
Sbjct: 367 FGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI-- 426

Query: 422 HSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQH 481
              Q   D RY E +N   +SHVCT P++ D        D++     +VTGAQ L V+ H
Sbjct: 427 --RQESHDRRYYEKVNWKNYSHVCTEPVESD-------DDLS-----VVTGAQ-LHVESH 486

Query: 482 DSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEA 541
             K VL L L +SRV  + +V+ +    +   +PKSG+ S + +     +AA K  P  A
Sbjct: 487 GFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISH--HFTAAQKPPPRPA 546

Query: 542 AVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQV 591
            V ++S ++ GGPP+  Q  +L+KFVD SEM +GPQ+SPG+W+V GARL +EKGKI L+V
Sbjct: 547 DVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKV 590

BLAST of Cucsa.012110 vs. Swiss-Prot
Match: NSL1_ARATH (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana GN=NSL1 PE=2 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 6.0e-127
Identity = 267/610 (43.77%), Postives = 370/610 (60.66%), Query Frame = 1

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCK----GQRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A    EKA++ +G G+DL SD R   CK    G R LV ++ ++ RDLI PG   V +VS
Sbjct: 11  AHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSR-LVEIDPTRNRDLIFPGGIVVNNVS 70

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN+MF F S CW  DA + 
Sbjct: 71  SSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSV 130

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG+FISL++  I R  L L DEV+  VPS+WD  A+A FIEKYGTH++VG+++GG
Sbjct: 131 KTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGG 190

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPI----------PQAFNLFDP 241
           +DV+ V+Q + S  EP E++  L+  GD+ F   C  P             P+  NL   
Sbjct: 191 KDVIHVKQMRKSNHEPEEIQKMLKHWGDERF---CVDPVESKSPASVYSGKPKEENLLQW 250

Query: 242 QLVAFGNFSSVSA-----KDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPII 301
            L  FG   S +       + I+ +  +RGG     SH  WL TV ++P+ I   F+PI 
Sbjct: 251 GLQPFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPIT 310

Query: 302 SLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSP 361
           SLL  +PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +SP
Sbjct: 311 SLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSP 370

Query: 362 SLQLNLMGPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQ 421
           SLQ +LMGPKLYVNT++V +  RPVTG+R +LEG K N LAIH+QHL   P  L  +++ 
Sbjct: 371 SLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSL--HLSH 430

Query: 422 QTYWHSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLV 481
              +   +   +  Y  P+    FSHVCT P++Y       +G  +   A IVT A  L 
Sbjct: 431 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQY-------NGARSDDTASIVTKA-WLE 490

Query: 482 VKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSL-TGTSAALKE 541
           VK    + VL L L +S   ++   ++ W   S++ S KSG+FS IST L TG S     
Sbjct: 491 VKGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRKSGVFSMISTRLSTGLSPNPAT 550

Query: 542 KPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGK 592
             P++ + ++S V+  GP   V+  +L+  VD  E+ +GP++ PG+W+V GA+L +E GK
Sbjct: 551 TKPQSKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGK 603

BLAST of Cucsa.012110 vs. Swiss-Prot
Match: CAD1_ARATH (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana GN=CAD1 PE=2 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 2.7e-111
Identity = 246/600 (41.00%), Postives = 339/600 (56.50%), Query Frame = 1

Query: 6   VEKALNSLGKGFDLTSDFRLKYCKGQRS--LVLLNQSQRRDLIVPGFGAVKDVSIDIKCD 65
           +  A+ +LG+GFD+TSD RL YCKG     LV + + Q RDL +     + +V  DI C 
Sbjct: 21  LRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCS 80

Query: 66  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCLGLD 125
           RG+    +  + +F++M+E FN +  + G IP G FN+MF + +G W  DA +TK L L 
Sbjct: 81  RGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALV 140

Query: 126 GYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDVVLV 185
           GYFI L++  + +  L L +E+R AVPS+WDP ++A FIE YGTHI+  ++IGGRDVV +
Sbjct: 141 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 200

Query: 186 RQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPIPQAFNLFDPQLVAFGNFSSVSAKD- 245
           RQ +SS L  SE++N++ ++              I   F+  + Q +       +  KD 
Sbjct: 201 RQHQSSPLPVSEIENYVNDM--------------IKHRFHEAESQSIT----GPLKYKDK 260

Query: 246 GIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIPGKGFLSHAINLYL 305
            I VI  +RGG     SH  W  TV  +PD I+  F PI+SLLE +PG   L+ AI LYL
Sbjct: 261 DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYL 320

Query: 306 RYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLMGPKLYVNTTQVIA 365
            YKPPI DLQYFLD+Q+ + WAP  ++L            SLQ +LMGPKL+++  QV  
Sbjct: 321 EYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTV 380

Query: 366 DNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSSQHIA--------- 425
             +PVTG+RL LEG K NRL+IH+QHL++ P +L      Q +W S   I          
Sbjct: 381 GRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKIL------QPHWDSHVPIGAPKWQGPEE 440

Query: 426 -DDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSKTVL 485
            D R+ EP+    FSHV T PI++     T  GD++     IVTGAQ L V    SK VL
Sbjct: 441 QDSRWFEPIKWKNFSHVSTSPIEHT---ETHIGDLS--GVHIVTGAQ-LGVWDFGSKNVL 500

Query: 486 HLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVIVDS 545
           HL LL+S+VP   I ++ W     D +P              +S  L+   P  +   + 
Sbjct: 501 HLKLLFSKVPGCTIRRSVW-----DHTP------------VASSGRLEPGGPSTSSSTEE 560

Query: 546 GVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFGLLH 593
                   +S Q  +L K VD SEM KGPQD PGHW+V GA+L +EKGKI L+VK+ LL+
Sbjct: 561 --------VSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLN 560

BLAST of Cucsa.012110 vs. TrEMBL
Match: A0A0A0KRG0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G551250 PE=4 SV=1)

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 592/603 (98.18%), Postives = 592/603 (98.18%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI
Sbjct: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP-----------IPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP           IPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI
Sbjct: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 593
           VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600

BLAST of Cucsa.012110 vs. TrEMBL
Match: E5GB62_CUCME (Putative uncharacterized protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 574/603 (95.19%), Postives = 583/603 (96.68%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP-----------IPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP           IPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADN+PVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 TVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 593
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 600

BLAST of Cucsa.012110 vs. TrEMBL
Match: A0A068UBD2_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00020695001 PE=4 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 6.7e-234
Identity = 405/603 (67.16%), Postives = 478/603 (79.27%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           M + IVE+ALNSLGKGFD+TSDFRLKYCKG+  LV LN+ +  +L+VPGFGA +DVSIDI
Sbjct: 1   MTEGIVERALNSLGKGFDITSDFRLKYCKGKERLVWLNERETTELLVPGFGAFRDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCD+GDRTRYQSDIL+F+QMSE FN+K S+PGK+PSG FNS+FGFQSG WATDA NTKCL
Sbjct: 61  KCDKGDRTRYQSDILDFSQMSEFFNQKSSVPGKVPSGMFNSVFGFQSGSWATDAANTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFI LFN HIDRYPL L+DEVR+AVPSTWDP A+ARFIEKYGTHIIVGLSIGG+DV
Sbjct: 121 GLDGYFIILFNVHIDRYPLVLADEVRNAVPSTWDPAALARFIEKYGTHIIVGLSIGGQDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPP----------PIPQAFNLFDPQLV 240
           VLVRQDKSS LEPS+LK+HL+ LGDQLF GAC+F P            PQAFN+FDP   
Sbjct: 181 VLVRQDKSSNLEPSQLKSHLDVLGDQLFTGACSFSPHQLKTREQKQKAPQAFNVFDPHPN 240

Query: 241 AFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIPG 300
            F +FSS++ KDGI VI SKRGG  + S+HCEW+ TV   PDAIHF FIPI SLL+ +PG
Sbjct: 241 PFNSFSSITTKDGITVICSKRGGDTSTSTHCEWILTVPSMPDAIHFNFIPITSLLKGVPG 300

Query: 301 KGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNIS-TSPSLQLNLM 360
           KGFLSHAINLYLRYKPPI DLQYFLDFQ HK+WAP+HNDLPLGP  N S  +P+L  N M
Sbjct: 301 KGFLSHAINLYLRYKPPINDLQYFLDFQAHKMWAPVHNDLPLGPARNKSRQNPTLHFNFM 360

Query: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTT+VI   RPVTGMRLYLEGMK NRLA+H+QHL   P+L ++ I     W  +
Sbjct: 361 GPKLYVNTTKVIVGKRPVTGMRLYLEGMKCNRLAVHLQHLSNAPVLFQDKINDPLTWRGT 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           + I DDRY+E +   KFSH+CT P++YDP WAT + +     AFIVTGAQ + VK+  SK
Sbjct: 421 EEITDDRYMEAVQSKKFSHICTAPVRYDPKWATKTDE-----AFIVTGAQ-IHVKKESSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V N  +VQ+NW +  S+ S K  IFSAISTS++GT    KEK P  AV+
Sbjct: 481 TVLHLRLLYSKVSNCCVVQSNWMQCPSENSIKWSIFSAISTSISGTLD--KEKMP--AVV 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 593
           VDS V+  GPP+ VQ Q+L+KFVD S++ KGPQD+PGHW+V GA+L+LEKGKICL VKF 
Sbjct: 541 VDSAVYPTGPPVPVQTQKLLKFVDTSQLCKGPQDNPGHWLVTGAKLDLEKGKICLHVKFS 593

BLAST of Cucsa.012110 vs. TrEMBL
Match: D7T8J2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g05950 PE=4 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 9.1e-231
Identity = 398/604 (65.89%), Postives = 480/604 (79.47%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           M D I E AL SLGKGFDLTSDFRLKYCKG+R LVLLN++++RDL++PGFG ++DVS+DI
Sbjct: 1   MKDGIAEMALASLGKGFDLTSDFRLKYCKGKRPLVLLNEAEKRDLLLPGFGLLRDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCD+GDR RYQSDIL+F+QMSELFN+K S+PGKIPSG FN+MFGFQSG WA DA  TK L
Sbjct: 61  KCDKGDRVRYQSDILDFHQMSELFNQKASVPGKIPSGMFNAMFGFQSGSWARDAAKTKYL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
            LDGYFI LF+ HI RYPL LS++VR+AVPSTWDP A+ARFIEKYGTHI+VGLS+GG+DV
Sbjct: 121 ALDGYFIVLFSLHIHRYPLLLSEDVRNAVPSTWDPCALARFIEKYGTHIVVGLSVGGQDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPP-----------PIPQAFNLFDPQL 240
           +LVRQDKSS + PSELK H+EELGDQLF G+CTF P             PQAFNLFDPQ 
Sbjct: 181 LLVRQDKSSSMGPSELKQHIEELGDQLFTGSCTFSPIHAKSKEHNKNKAPQAFNLFDPQP 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           + F +FSSVSAKDGI VI+SKRGG   A SHCEWL TV   PDA+ F FIPI SLL+ +P
Sbjct: 241 LPFNSFSSVSAKDGITVISSKRGGEAFAESHCEWLLTVPSMPDAVQFSFIPITSLLKGVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNIS-TSPSLQLNL 360
           G+GFLSHAINLYLRYKPPI+DLQYFL+FQVHK+WAP+H+DLPLGPT N++  SP+L+ NL
Sbjct: 301 GRGFLSHAINLYLRYKPPISDLQYFLEFQVHKLWAPVHSDLPLGPTTNMAVASPALRFNL 360

Query: 361 MGPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHS 420
           MGPKLY+NT+QV    RPVTGMRLYLEGMK NRLAIH+QHL  TP++L+N I     W  
Sbjct: 361 MGPKLYINTSQVTVGRRPVTGMRLYLEGMKCNRLAIHLQHLSNTPVMLQNLIDDTPMWRG 420

Query: 421 SQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDS 480
           S+ + DDR++EP++  KFSH+CT  +KYDP+W+T         +FIVTG Q L VK+HDS
Sbjct: 421 SEEVTDDRFLEPIHWKKFSHICTASVKYDPSWST-----REDVSFIVTGVQ-LHVKKHDS 480

Query: 481 KTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAV 540
           KTVLHL L +S+V NS+I Q NW + +S+ S KS  FSAISTS+TG+S    EK  E  V
Sbjct: 481 KTVLHLRLRFSKVRNSFIAQKNWMQGASEGSQKSSFFSAISTSITGSS----EK--EKQV 540

Query: 541 IVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKF 593
           +VDS V+  GPP+ VQ  +L+KFV++S++ KGPQDSPGHW+V GA L +EKGKI L VKF
Sbjct: 541 VVDSSVYPDGPPVLVQTPKLLKFVEMSQVSKGPQDSPGHWLVTGAMLQMEKGKIRLHVKF 592

BLAST of Cucsa.012110 vs. TrEMBL
Match: A0A061E8T4_THECC (MAC/Perforin domain-containing protein, putative OS=Theobroma cacao GN=TCM_011285 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 3.8e-229
Identity = 401/596 (67.28%), Postives = 475/596 (79.70%), Query Frame = 1

Query: 6   VEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDIKCDRG 65
           VEKAL+SLGKGFDLTSDFRLKYCKG+  L+LLN+++RR++ +PGFGAVKDV +DIKCD+G
Sbjct: 7   VEKALSSLGKGFDLTSDFRLKYCKGKERLILLNETERREIAIPGFGAVKDVPVDIKCDKG 66

Query: 66  DRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCLGLDGY 125
           DRTRYQSDIL+FNQM+E FN+KCS+PGKIPSG FN MFGFQS  WA DA NTK LGLDGY
Sbjct: 67  DRTRYQSDILDFNQMAEFFNQKCSLPGKIPSGQFNRMFGFQSSSWAKDAANTKFLGLDGY 126

Query: 126 FISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDVVLVRQ 185
            I LFN HIDRYPL LSDEV +AVPS WDP A+ARFIEKYGTH+IVGLSIGGRDVVLVRQ
Sbjct: 127 IIILFNLHIDRYPLLLSDEVVNAVPSAWDPCALARFIEKYGTHVIVGLSIGGRDVVLVRQ 186

Query: 186 DKSSKLEPSELKNHLEELGDQLFNGACTFPP-------PIPQAFNLFDPQLVAF-GNFSS 245
           DKSS LEPS+LK HL++LGDQLF G C+F P        +PQAFN+FD + VAF G  S+
Sbjct: 187 DKSSNLEPSQLKKHLDDLGDQLFTGTCSFTPKTKEQKQKVPQAFNIFDSEPVAFNGYHSN 246

Query: 246 VSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIPGKGFLSHA 305
           +S KDGIIVI SK+GG   A SHCEWLPTV + PDAIHF FIPI SLL+ +PGKGFLSHA
Sbjct: 247 ISTKDGIIVINSKKGGDLEAYSHCEWLPTVPQMPDAIHFNFIPITSLLKGVPGKGFLSHA 306

Query: 306 INLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTP-NISTSPSLQLNLMGPKLYVN 365
           INLYLRYKP I DL+YFLDFQ HKIWAPIHNDLPLGP+P   S++P+L  NLMGPKLYVN
Sbjct: 307 INLYLRYKPSIEDLRYFLDFQAHKIWAPIHNDLPLGPSPKRASSTPALHFNLMGPKLYVN 366

Query: 366 TTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSSQHIADDR 425
           T+QV    RPVTGMRLYLEGMK NRLAIH+QHL   P   EN +    YW +S+  AD R
Sbjct: 367 TSQVTVGMRPVTGMRLYLEGMKCNRLAIHLQHLCNLPKTFENKMDDIQYWQASEDTADVR 426

Query: 426 YVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSKTVLHLGL 485
           + E ++  KFSH+CT P+KY+P W  T+G     A FIVTGAQ L VK+HDSK+VLHL L
Sbjct: 427 FFEAIHHKKFSHICTAPVKYNPKW--TAG---KDAVFIVTGAQ-LHVKKHDSKSVLHLRL 486

Query: 486 LYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVIVDSGVFH 545
           L+S+V +S++VQ++WA+ +S  S +SG+ SAIS SLTG    +KEK  +  V+VDS V+ 
Sbjct: 487 LFSKVSDSFLVQSSWAQAASGFSQRSGLLSAISQSLTGN--PVKEK--KEVVVVDSAVYP 546

Query: 546 GGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFGLLH 593
            GPP+ VQ  +L+KFV+ S++ KGPQDSPG+W+V GARL LEKGKI L VKF LL+
Sbjct: 547 TGPPVPVQTPKLLKFVETSQLCKGPQDSPGYWLVTGARLQLEKGKISLHVKFSLLN 592

BLAST of Cucsa.012110 vs. TAIR10
Match: AT1G14780.1 (AT1G14780.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 678.7 bits (1750), Expect = 3.2e-195
Identity = 347/616 (56.33%), Postives = 442/616 (71.75%), Query Frame = 1

Query: 4   EIVEKALNSLGKGFDLTSDFRLKYCK------GQRSLVLLNQSQRRDLIVPGFGAVKDVS 63
           +++E A+ SLGKGFDLT+DFRLKYCK      G   LV+L+Q+Q R+L +PGFG  ++VS
Sbjct: 7   DVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVS 66

Query: 64  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 123
            DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FN+ FGFQSG WATDA N 
Sbjct: 67  ADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANV 126

Query: 124 KCLGLDGYFISLFNAHIDR-YPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIG 183
           K LGLD   ++LFN HI     LRL+D VR+AVPS+WDP  +ARFIE+YGTH+I G+S+G
Sbjct: 127 KSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVG 186

Query: 184 GRDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFP--------------PPIPQAF 243
           G+DVV+VRQDKSS L+   L++HL +LGDQLF G+C                 P  P+AF
Sbjct: 187 GQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 246

Query: 244 NLFDP-QLVAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPI 303
           N+FD  Q VAF NFS +++++GI VI +KRGG   A SH EWL TV + PDAI+F FIPI
Sbjct: 247 NVFDDKQTVAFNNFS-INSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 306

Query: 304 ISLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTS 363
            SLL+ +PG G LSHA++LYLRYKPP+ DLQYFLDF   + WAP+HNDLP G  PN++++
Sbjct: 307 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 366

Query: 364 -PSLQLNLMGPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNI 423
            P+L +N MGPKLYVNTT V ++  PVTGMR +LEG K NRLAIH+QHL  T   +   I
Sbjct: 367 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 426

Query: 424 TQQTYWHSSQHIAD-DRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNS--PAAFIVTG 483
           T +  W  S  I D DRY EPLN  KFSHVCT+P+KYDP W  T+ +  S    AFIVTG
Sbjct: 427 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 486

Query: 484 AQLLVVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSA 543
           AQ L VK+H SK+VLHL L Y++V + Y+VQ +W       S KSGIFS++S  LT  S 
Sbjct: 487 AQ-LEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSV 546

Query: 544 ALKE-KPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLN 593
                +  +  V++DSGVF GGPP+     ++VKFVDLS++ +GPQ SPGHW+V G RL 
Sbjct: 547 HHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLY 606

BLAST of Cucsa.012110 vs. TAIR10
Match: AT4G24290.2 (AT4G24290.2 MAC/Perforin domain-containing protein)

HSP 1 Score: 490.0 bits (1260), Expect = 2.1e-138
Identity = 274/604 (45.36%), Postives = 378/604 (62.58%), Query Frame = 1

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCKG----QRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A +  E A+ S+G G+DL  D RLKYCKG     R L +       ++++PG  ++ +VS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+R R++SDIL F QM+E FN++ S+ GKIPSG FN+MF F S CW  DA  T
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSS-CWQKDAAYT 126

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG FISL++  +D+  + L + V+ AVPSTWDP A+ARFI+ YGTHIIV + +GG
Sbjct: 127 KNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGG 186

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPIPQAFNLFDP-----QLVAF 241
           +DV+  +Q  SSKL+P +L+  L+E+ D+ F  A        +            Q + F
Sbjct: 187 KDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRF 246

Query: 242 GNFSSVSA---KDGIIVITSKRGGSPTAS-SHCEWLPTVHESPDAIHFQFIPIISLLECI 301
            + SS+ +   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL  +
Sbjct: 247 ADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGV 306

Query: 302 PGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNL 361
           PG GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +  SLQ + 
Sbjct: 307 PGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSF 366

Query: 362 MGPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLL--LENNITQQTYW 421
            GPKLYVNTT V    RP+TGMRLYLEG +SNRLAIH+QHL + P +  LE+++ +    
Sbjct: 367 FGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI-- 426

Query: 422 HSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQH 481
              Q   D RY E +N   +SHVCT P++ D        D++     +VTGAQ L V+ H
Sbjct: 427 --RQESHDRRYYEKVNWKNYSHVCTEPVESD-------DDLS-----VVTGAQ-LHVESH 486

Query: 482 DSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEA 541
             K VL L L +SRV  + +V+ +    +   +PKSG+ S + +     +AA K  P  A
Sbjct: 487 GFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISH--HFTAAQKPPPRPA 546

Query: 542 AVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQV 591
            V ++S ++ GGPP+  Q  +L+KFVD SEM +GPQ+SPG+W+V GARL +EKGKI L+V
Sbjct: 547 DVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKV 590

BLAST of Cucsa.012110 vs. TAIR10
Match: AT1G28380.1 (AT1G28380.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 456.1 bits (1172), Expect = 3.4e-128
Identity = 267/610 (43.77%), Postives = 370/610 (60.66%), Query Frame = 1

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCK----GQRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A    EKA++ +G G+DL SD R   CK    G R LV ++ ++ RDLI PG   V +VS
Sbjct: 11  AHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSR-LVEIDPTRNRDLIFPGGIVVNNVS 70

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN+MF F S CW  DA + 
Sbjct: 71  SSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSV 130

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG+FISL++  I R  L L DEV+  VPS+WD  A+A FIEKYGTH++VG+++GG
Sbjct: 131 KTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGG 190

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPI----------PQAFNLFDP 241
           +DV+ V+Q + S  EP E++  L+  GD+ F   C  P             P+  NL   
Sbjct: 191 KDVIHVKQMRKSNHEPEEIQKMLKHWGDERF---CVDPVESKSPASVYSGKPKEENLLQW 250

Query: 242 QLVAFGNFSSVSA-----KDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPII 301
            L  FG   S +       + I+ +  +RGG     SH  WL TV ++P+ I   F+PI 
Sbjct: 251 GLQPFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPIT 310

Query: 302 SLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSP 361
           SLL  +PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +SP
Sbjct: 311 SLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSP 370

Query: 362 SLQLNLMGPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQ 421
           SLQ +LMGPKLYVNT++V +  RPVTG+R +LEG K N LAIH+QHL   P  L  +++ 
Sbjct: 371 SLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSL--HLSH 430

Query: 422 QTYWHSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLV 481
              +   +   +  Y  P+    FSHVCT P++Y       +G  +   A IVT A  L 
Sbjct: 431 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQY-------NGARSDDTASIVTKA-WLE 490

Query: 482 VKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSL-TGTSAALKE 541
           VK    + VL L L +S   ++   ++ W   S++ S KSG+FS IST L TG S     
Sbjct: 491 VKGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRKSGVFSMISTRLSTGLSPNPAT 550

Query: 542 KPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGK 592
             P++ + ++S V+  GP   V+  +L+  VD  E+ +GP++ PG+W+V GA+L +E GK
Sbjct: 551 TKPQSKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGK 603

BLAST of Cucsa.012110 vs. TAIR10
Match: AT1G29690.1 (AT1G29690.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 404.1 bits (1037), Expect = 1.5e-112
Identity = 246/600 (41.00%), Postives = 339/600 (56.50%), Query Frame = 1

Query: 6   VEKALNSLGKGFDLTSDFRLKYCKGQRS--LVLLNQSQRRDLIVPGFGAVKDVSIDIKCD 65
           +  A+ +LG+GFD+TSD RL YCKG     LV + + Q RDL +     + +V  DI C 
Sbjct: 21  LRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCS 80

Query: 66  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCLGLD 125
           RG+    +  + +F++M+E FN +  + G IP G FN+MF + +G W  DA +TK L L 
Sbjct: 81  RGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALV 140

Query: 126 GYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDVVLV 185
           GYFI L++  + +  L L +E+R AVPS+WDP ++A FIE YGTHI+  ++IGGRDVV +
Sbjct: 141 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 200

Query: 186 RQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPIPQAFNLFDPQLVAFGNFSSVSAKD- 245
           RQ +SS L  SE++N++ ++              I   F+  + Q +       +  KD 
Sbjct: 201 RQHQSSPLPVSEIENYVNDM--------------IKHRFHEAESQSIT----GPLKYKDK 260

Query: 246 GIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIPGKGFLSHAINLYL 305
            I VI  +RGG     SH  W  TV  +PD I+  F PI+SLLE +PG   L+ AI LYL
Sbjct: 261 DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYL 320

Query: 306 RYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLMGPKLYVNTTQVIA 365
            YKPPI DLQYFLD+Q+ + WAP  ++L            SLQ +LMGPKL+++  QV  
Sbjct: 321 EYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTV 380

Query: 366 DNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSSQHIA--------- 425
             +PVTG+RL LEG K NRL+IH+QHL++ P +L      Q +W S   I          
Sbjct: 381 GRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKIL------QPHWDSHVPIGAPKWQGPEE 440

Query: 426 -DDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSKTVL 485
            D R+ EP+    FSHV T PI++     T  GD++     IVTGAQ L V    SK VL
Sbjct: 441 QDSRWFEPIKWKNFSHVSTSPIEHT---ETHIGDLS--GVHIVTGAQ-LGVWDFGSKNVL 500

Query: 486 HLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVIVDS 545
           HL LL+S+VP   I ++ W     D +P              +S  L+   P  +   + 
Sbjct: 501 HLKLLFSKVPGCTIRRSVW-----DHTP------------VASSGRLEPGGPSTSSSTEE 560

Query: 546 GVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFGLLH 593
                   +S Q  +L K VD SEM KGPQD PGHW+V GA+L +EKGKI L+VK+ LL+
Sbjct: 561 --------VSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLN 560

BLAST of Cucsa.012110 vs. NCBI nr
Match: gi|449450151|ref|XP_004142827.1| (PREDICTED: MACPF domain-containing protein At1g14780 [Cucumis sativus])

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 592/603 (98.18%), Postives = 592/603 (98.18%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI
Sbjct: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP-----------IPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP           IPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI
Sbjct: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 593
           VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600

BLAST of Cucsa.012110 vs. NCBI nr
Match: gi|307135833|gb|ADN33704.1| (hypothetical protein [Cucumis melo subsp. melo])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 574/603 (95.19%), Postives = 583/603 (96.68%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP-----------IPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP           IPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADN+PVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 TVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 593
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 600

BLAST of Cucsa.012110 vs. NCBI nr
Match: gi|659072766|ref|XP_008467045.1| (PREDICTED: LOW QUALITY PROTEIN: MACPF domain-containing protein At1g14780-like [Cucumis melo])

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 573/603 (95.02%), Postives = 582/603 (96.52%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 91  MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 150

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 151 KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 210

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 211 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 270

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP-----------IPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP           IPQAFNLFDPQL
Sbjct: 271 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 330

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 331 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 390

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 391 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 450

Query: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADN+PVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 451 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 510

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 511 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 570

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
            VLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 571 XVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 630

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 593
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 631 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 690

BLAST of Cucsa.012110 vs. NCBI nr
Match: gi|661890995|emb|CDP05572.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 818.1 bits (2112), Expect = 9.6e-234
Identity = 405/603 (67.16%), Postives = 478/603 (79.27%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           M + IVE+ALNSLGKGFD+TSDFRLKYCKG+  LV LN+ +  +L+VPGFGA +DVSIDI
Sbjct: 1   MTEGIVERALNSLGKGFDITSDFRLKYCKGKERLVWLNERETTELLVPGFGAFRDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCD+GDRTRYQSDIL+F+QMSE FN+K S+PGK+PSG FNS+FGFQSG WATDA NTKCL
Sbjct: 61  KCDKGDRTRYQSDILDFSQMSEFFNQKSSVPGKVPSGMFNSVFGFQSGSWATDAANTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFI LFN HIDRYPL L+DEVR+AVPSTWDP A+ARFIEKYGTHIIVGLSIGG+DV
Sbjct: 121 GLDGYFIILFNVHIDRYPLVLADEVRNAVPSTWDPAALARFIEKYGTHIIVGLSIGGQDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPP----------PIPQAFNLFDPQLV 240
           VLVRQDKSS LEPS+LK+HL+ LGDQLF GAC+F P            PQAFN+FDP   
Sbjct: 181 VLVRQDKSSNLEPSQLKSHLDVLGDQLFTGACSFSPHQLKTREQKQKAPQAFNVFDPHPN 240

Query: 241 AFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIPG 300
            F +FSS++ KDGI VI SKRGG  + S+HCEW+ TV   PDAIHF FIPI SLL+ +PG
Sbjct: 241 PFNSFSSITTKDGITVICSKRGGDTSTSTHCEWILTVPSMPDAIHFNFIPITSLLKGVPG 300

Query: 301 KGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNIS-TSPSLQLNLM 360
           KGFLSHAINLYLRYKPPI DLQYFLDFQ HK+WAP+HNDLPLGP  N S  +P+L  N M
Sbjct: 301 KGFLSHAINLYLRYKPPINDLQYFLDFQAHKMWAPVHNDLPLGPARNKSRQNPTLHFNFM 360

Query: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTT+VI   RPVTGMRLYLEGMK NRLA+H+QHL   P+L ++ I     W  +
Sbjct: 361 GPKLYVNTTKVIVGKRPVTGMRLYLEGMKCNRLAVHLQHLSNAPVLFQDKINDPLTWRGT 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           + I DDRY+E +   KFSH+CT P++YDP WAT + +     AFIVTGAQ + VK+  SK
Sbjct: 421 EEITDDRYMEAVQSKKFSHICTAPVRYDPKWATKTDE-----AFIVTGAQ-IHVKKESSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V N  +VQ+NW +  S+ S K  IFSAISTS++GT    KEK P  AV+
Sbjct: 481 TVLHLRLLYSKVSNCCVVQSNWMQCPSENSIKWSIFSAISTSISGTLD--KEKMP--AVV 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 593
           VDS V+  GPP+ VQ Q+L+KFVD S++ KGPQD+PGHW+V GA+L+LEKGKICL VKF 
Sbjct: 541 VDSAVYPTGPPVPVQTQKLLKFVDTSQLCKGPQDNPGHWLVTGAKLDLEKGKICLHVKFS 593

BLAST of Cucsa.012110 vs. NCBI nr
Match: gi|297737612|emb|CBI26813.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 807.7 bits (2085), Expect = 1.3e-230
Identity = 398/604 (65.89%), Postives = 480/604 (79.47%), Query Frame = 1

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           M D I E AL SLGKGFDLTSDFRLKYCKG+R LVLLN++++RDL++PGFG ++DVS+DI
Sbjct: 1   MKDGIAEMALASLGKGFDLTSDFRLKYCKGKRPLVLLNEAEKRDLLLPGFGLLRDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCD+GDR RYQSDIL+F+QMSELFN+K S+PGKIPSG FN+MFGFQSG WA DA  TK L
Sbjct: 61  KCDKGDRVRYQSDILDFHQMSELFNQKASVPGKIPSGMFNAMFGFQSGSWARDAAKTKYL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
            LDGYFI LF+ HI RYPL LS++VR+AVPSTWDP A+ARFIEKYGTHI+VGLS+GG+DV
Sbjct: 121 ALDGYFIVLFSLHIHRYPLLLSEDVRNAVPSTWDPCALARFIEKYGTHIVVGLSVGGQDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPP-----------PIPQAFNLFDPQL 240
           +LVRQDKSS + PSELK H+EELGDQLF G+CTF P             PQAFNLFDPQ 
Sbjct: 181 LLVRQDKSSSMGPSELKQHIEELGDQLFTGSCTFSPIHAKSKEHNKNKAPQAFNLFDPQP 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           + F +FSSVSAKDGI VI+SKRGG   A SHCEWL TV   PDA+ F FIPI SLL+ +P
Sbjct: 241 LPFNSFSSVSAKDGITVISSKRGGEAFAESHCEWLLTVPSMPDAVQFSFIPITSLLKGVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNIS-TSPSLQLNL 360
           G+GFLSHAINLYLRYKPPI+DLQYFL+FQVHK+WAP+H+DLPLGPT N++  SP+L+ NL
Sbjct: 301 GRGFLSHAINLYLRYKPPISDLQYFLEFQVHKLWAPVHSDLPLGPTTNMAVASPALRFNL 360

Query: 361 MGPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHS 420
           MGPKLY+NT+QV    RPVTGMRLYLEGMK NRLAIH+QHL  TP++L+N I     W  
Sbjct: 361 MGPKLYINTSQVTVGRRPVTGMRLYLEGMKCNRLAIHLQHLSNTPVMLQNLIDDTPMWRG 420

Query: 421 SQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDS 480
           S+ + DDR++EP++  KFSH+CT  +KYDP+W+T         +FIVTG Q L VK+HDS
Sbjct: 421 SEEVTDDRFLEPIHWKKFSHICTASVKYDPSWST-----REDVSFIVTGVQ-LHVKKHDS 480

Query: 481 KTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAV 540
           KTVLHL L +S+V NS+I Q NW + +S+ S KS  FSAISTS+TG+S    EK  E  V
Sbjct: 481 KTVLHLRLRFSKVRNSFIAQKNWMQGASEGSQKSSFFSAISTSITGSS----EK--EKQV 540

Query: 541 IVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKF 593
           +VDS V+  GPP+ VQ  +L+KFV++S++ KGPQDSPGHW+V GA L +EKGKI L VKF
Sbjct: 541 VVDSSVYPDGPPVLVQTPKLLKFVEMSQVSKGPQDSPGHWLVTGAMLQMEKGKIRLHVKF 592