Cucsa.011780 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.011780 vs. Swiss-Prot
Match: SMC4_ARATH (Structural maintenance of chromosomes protein 4 OS=Arabidopsis thaliana GN=SMC4 PE=1 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 1.7e-264
Identity = 477/699 (68.24%), Postives = 568/699 (81.26%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTAC GLDYIVVET+ +AQACVELLR+ NLG ATFMILEKQ DH+ KLK KV 
Sbjct: 569  AKYDVAISTACAGLDYIVVETTSSAQACVELLRKGNLGFATFMILEKQTDHIHKLKEKVK 628

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPE VPRLFDL++V+D+RMKLAF+AALGNTVVAKDL+QATRIAYGGNR+FRRVV LDGAL
Sbjct: 629  TPEDVPRLFDLVRVKDERMKLAFYAALGNTVVAKDLDQATRIAYGGNREFRRVVALDGAL 688

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
             EKSGTMSGGG   RGG+MGTSIR+  VS EA   AE +LS +VD LN IR ++ +AV+ 
Sbjct: 689  FEKSGTMSGGGGKARGGRMGTSIRATGVSGEAVANAENELSKIVDMLNNIREKVGNAVRQ 748

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            Y+ +E  V  LEM LAKSQ++I+SL S+H+YLEKQL+SLEAAS+PK DE++RL+EL+  I
Sbjct: 749  YRAAENEVSGLEMELAKSQREIESLNSEHNYLEKQLASLEAASQPKTDEIDRLKELKKII 808

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
             +EEKEI  L  GSK+L +K   +Q+ IENAGGE+LK QK+KV KIQ+DI+K  T+INR 
Sbjct: 809  SKEEKEIENLEKGSKQLKDK---LQTNIENAGGEKLKGQKAKVEKIQTDIDKNNTEINRC 868

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
             VQIE++Q  +KKLTK IE++ +EKERLE EK NL   FKDI  KAF +QE YK+T++LI
Sbjct: 869  NVQIETNQKLIKKLTKGIEEATREKERLEGEKENLHVTFKDITQKAFEIQETYKKTQQLI 928

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
               ++V   +K++Y  +KK++DELK S VD E+K+QD+KK Y ELE++EKGY+ KL+DLQ
Sbjct: 929  DEHKDVLTGAKSDYENLKKSVDELKASRVDAEFKVQDMKKKYNELEMREKGYKKKLNDLQ 988

Query: 440  TALSKHMDQIHKDLVDPEKLQATLAE-DIVECRDLKRALEMVTLLDAQLKEMNPNLDSIT 499
             A +KHM+QI KDLVDP+KLQATL + ++ E  DLKRALEMV LL+AQLKE+NPNLDSI 
Sbjct: 989  IAFTKHMEQIQKDLVDPDKLQATLMDNNLNEACDLKRALEMVALLEAQLKELNPNLDSIA 1048

Query: 500  EYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFY 559
            EYR KVE+YN RV++LN+VTQ+RD  +KQYDEL+K+R  +F + F               
Sbjct: 1049 EYRSKVELYNGRVDELNSVTQERDDTRKQYDELRKRRLDEFMAGFNT------------- 1108

Query: 560  LSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 619
            +SL    M++             MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI
Sbjct: 1109 ISLKLKEMYQ-------------MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 1168

Query: 620  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 679
            ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII
Sbjct: 1169 ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 1228

Query: 680  ISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEK 718
            ISLRNNMFELADRLVGIYKT+NCTKSITINP SF+VC+K
Sbjct: 1229 ISLRNNMFELADRLVGIYKTDNCTKSITINPGSFAVCQK 1238

HSP 2 Score: 58.9 bits (141), Expect = 2.6e-07
Identity = 83/382 (21.73%), Postives = 167/382 (43.72%), Query Frame = 1

Query: 204 EKAVEQLEMLLAKSQ--QDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFILE 263
           E  +E LE ++  ++  + ID L  Q   L +  S +    K  + E + LE L++   E
Sbjct: 190 EGFLEYLEDIIGTNKYVEKIDELNKQLETLNESRSGVVQMVKLAEKERDNLEGLKD---E 249

Query: 264 EE----KEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDIN 323
            E    KE+S L     K  EKA ++  +   A          K+T+ +  +      + 
Sbjct: 250 AETYMLKELSHL-----KWQEKATKMAYEDTVA----------KITEQRDSLQNLENSLK 309

Query: 324 RYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEK 383
             +V+++     +KK     E  KK +E L+ E    + KFK+ E +    +E+ K  ++
Sbjct: 310 DERVKMDESNEELKKFESVHEKHKKRQEVLDNELRACKEKFKEFERQDVKHREDLKHVKQ 369

Query: 384 LIHLQEE--------VCDTSKANYN------KVKKTMDELKGSEVDTEYKLQDLKKLYKE 443
            I   E+        + D +K + +      K+++ + +L+   +D E KL+++K + K 
Sbjct: 370 KIKKLEDKLEKDSSKIGDMTKESEDSSNLIPKLQENIPKLQKVLLDEEKKLEEIKAIAK- 429

Query: 444 LELKEKGYRTKLDDLQTALSKHMDQIHKDL-VDPEKLQATLAEDIVECRDLKRALEMVTL 503
             ++ +GYR++L    T +   ++   KDL V   KL    +E  +  +  + AL+  T 
Sbjct: 430 --VETEGYRSEL----TKIRAELEPWEKDLIVHRGKLDVASSESELLSKKHEAALKAFTD 489

Query: 504 LDAQLKEMN----PNLDSITEY-----RRKVEVYNERVEDLNTVTQQRDIVKKQYDELKK 556
              QL +++        + T +     ++K E    R  +  ++ +Q  +V ++    +K
Sbjct: 490 AQKQLSDISTRKKEKAAATTSWKADIKKKKQEAIEARKVEEESLKEQETLVPQEQAAREK 546

BLAST of Cucsa.011780 vs. Swiss-Prot
Match: SMC4_DICDI (Structural maintenance of chromosomes protein 4 OS=Dictyostelium discoideum GN=smc4 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 6.6e-136
Identity = 287/712 (40.31%), Postives = 424/712 (59.55%), Query Frame = 1

Query: 21   KYDVAISTAC-PGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 80
            KYDVAISTA    +D I+VET+ AA+ACVELLR+ENLG ATFMILE  +++  +    V 
Sbjct: 710  KYDVAISTAAFSQMDNIIVETTAAAEACVELLRKENLGRATFMILEN-LEYQRQNLGPVQ 769

Query: 81   TPEGVPRLFDLIKVQDDRM-KLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGA 140
            TP   PRLFDLIK++D++    AFF A+G+T+VA  L++AT+IAYG  R   RVVTLDG+
Sbjct: 770  TPNNTPRLFDLIKMKDEKKYATAFFTAVGHTLVADTLDEATKIAYGAKR--HRVVTLDGS 829

Query: 141  LLEKSGTMSGGGRMPRGGKMGTSIRS-ASVSKEAFVKAEKDLSDMVDALNRIRLRIADAV 200
            L++ SG MSGGG  PR G M + ++      K+  ++ + +LS +   L + R  + +  
Sbjct: 830  LIDTSGAMSGGGLKPRVGAMNSKLKGDPKEDKKKLIELQDNLSQLDSDLRQCRDELVEIE 889

Query: 201  QLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRN 260
               Q ++    +LE+ L K   DI +  ++   L K +  L+  +K   ++  +++ ++ 
Sbjct: 890  NQIQQAQNRRSELELELPKMDMDIKAAITKCEELTKVIPQLKNKAKLSTEKKEQIDSIKE 949

Query: 261  FILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDIN 320
             ++ ++K + ++     KL  +  EIQ+ I N GG +LK QK+KV  +QS I+  +T+  
Sbjct: 950  SLIVDQKSLDKVQEKVNKLESEVQEIQNSILNVGGPQLKMQKNKVESLQSRIDSNQTNTT 1009

Query: 321  RYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEK 380
            +  VQI+S   +M+K  K + ++ KEK+  E     +  K+K +E +     E  +   +
Sbjct: 1010 KANVQIKSLAKSMEKSIKILNENTKEKDENEAALAEILEKYKSLEKENLKATEAMEAVSE 1069

Query: 381  LIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDD 440
             +  +EE     +  + K KK ++++K S    E ++++ K L  E          K  +
Sbjct: 1070 QLREKEEETKEIRKEHEKAKKVIEKIKVSNSKLETQIEEFKTLINE----------KQAE 1129

Query: 441  LQTALSKHMDQ-----IHKDLVDPEKLQATLA-------EDIVECRDLKRALEMVTLLDA 500
            +   LSK  +Q     I+KD VD   +    A       E  +E  + +  +  +  L  
Sbjct: 1130 IADCLSKFANQAKKAKIYKDYVDESLINQVSAILTPEEIEQYMEATEQQNLIAKIHELTT 1189

Query: 501  QL----KEMNPNLDSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSS 560
            Q+    KE N N++ + ++++K + Y+ R  + + + ++RD + K+Y+ L+K R  +F +
Sbjct: 1190 QIQKISKENNVNIEVVKDFQKKEQEYHSRKAEFDEIEKERDNLSKRYESLRKNRLDEFMA 1249

Query: 561  LFKKCTRFSSKLILSFYLSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFS 620
             F   T                          + L+    MITLGGDAELE++D  DPF 
Sbjct: 1250 GFTIIT--------------------------MKLKEIYQMITLGGDAELEIIDREDPFQ 1309

Query: 621  EGVVFSVRPPKKSWKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVS 680
            EG+ FSVRPPKKSWKNI+NLSGGEKTLSSLALVFALHHYKP  LYVMDEIDAALDFKNVS
Sbjct: 1310 EGISFSVRPPKKSWKNISNLSGGEKTLSSLALVFALHHYKPNALYVMDEIDAALDFKNVS 1369

Query: 681  IVGHYVKDRTKDAQFIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFS 714
            I+ +Y+K+RTK+AQFIIISLRN MFELADRLVGIYKT+NCTKS+TINP SF+
Sbjct: 1370 IIANYIKERTKNAQFIIISLRNYMFELADRLVGIYKTDNCTKSVTINPNSFT 1382

BLAST of Cucsa.011780 vs. Swiss-Prot
Match: SMC4_XENLA (Structural maintenance of chromosomes protein 4 OS=Xenopus laevis GN=smc4 PE=1 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.2e-126
Identity = 273/696 (39.22%), Postives = 420/696 (60.34%), Query Frame = 1

Query: 21   KYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVST 80
            KYDVAIS++C  LD+IVV+T   AQ CV  L+++N+GVATF+ L+K       L  K+ T
Sbjct: 622  KYDVAISSSCGALDHIVVDTIDTAQECVNFLKKQNVGVATFIGLDKMKVWEKGLN-KIQT 681

Query: 81   PEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGALL 140
            PE +PRLFD++KV+D+++K AF+ AL +T+VA +L+QATR+A+  ++ +R VVTL G ++
Sbjct: 682  PENIPRLFDMVKVKDEQIKPAFYFALRDTIVANNLDQATRVAFQKDKRWR-VVTLQGQII 741

Query: 141  EKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQLY 200
            E+SGTM+GGG     G+MG+S+    +S +   K E  L         I+ R A   +  
Sbjct: 742  EQSGTMTGGGGKVMKGRMGSSVM-VEISDDQLQKMENKLKTDTTRATEIQDRKAHLEEEV 801

Query: 201  QVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFIL 260
                +A  +++    K    + SL+ Q  +L+ Q+  LE        + N+ +++   + 
Sbjct: 802  AKLRQATREMKNTFEKYTASLQSLSEQEVHLKAQVKELEVNVAAAAPDKNQQKQMEKNLE 861

Query: 261  EEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRYK 320
              +KE  ++   + K+  +   +   I +    +LKAQ+ K+ K+  +I++  + I + +
Sbjct: 862  TLKKEYEKVAEKAGKVEAEVKRLHKLIVDINNHKLKAQQDKLDKVTKEIDECASAITKAQ 921

Query: 321  VQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETE-KLI 380
            V I++    +KK  +A+  ++KE    ++    L    K +E KA  V    KE E  L 
Sbjct: 922  VSIKTADRNLKKSEEAVARTEKEIVANDKSIEELTEDLKKLEEKATTVMNECKEAECSLP 981

Query: 381  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQ----DLKKLYKELELKEKGYRTKL 440
             +QE+            +  + E+K  + + E+ LQ    +++   ++++     +++K+
Sbjct: 982  EVQEQH-----------RSLLQEIKAIQ-EKEHALQKEALNIRLNIEQIDSHIAEHQSKI 1041

Query: 441  DDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVEC-RDLKRALEMVTLLDAQLKEMNPNL 500
               Q  ++K    +HK    PE++   LA++ +E  +D  + +  + LL+A+  EM PNL
Sbjct: 1042 KYWQKEITKI--SLHKIEDIPEEVLPGLAQEELEAIKDPDQIINQIALLEAKSHEMKPNL 1101

Query: 501  DSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLI 560
             +I EY++K E+Y +RV +L+ +T +RD  ++ Y++L+K+R  +F + F   T   +KL 
Sbjct: 1102 GAIAEYKKKEELYLQRVAELDEITNERDSFRRAYEDLRKQRLNEFMAGFNIIT---NKLK 1161

Query: 561  LSFYLSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKS 620
             ++                        M+TLGGDAELELVDSLDPFSEG++FSVRPPKKS
Sbjct: 1162 ENYQ-----------------------MLTLGGDAELELVDSLDPFSEGIMFSVRPPKKS 1221

Query: 621  WKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDA 680
            WK I NLSGGEKTLSSLALVFALHHYKPTPLY MDEIDAALDFKNVSIV  Y+ ++TK+A
Sbjct: 1222 WKKIFNLSGGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNA 1274

Query: 681  QFIIISLRNNMFELADRLVGIYKTNNCTKSITINPR 711
            QFIIISLRNNMFE+ADRL+GIYKT+N TKS+  NP+
Sbjct: 1282 QFIIISLRNNMFEIADRLIGIYKTHNTTKSVATNPK 1274

HSP 2 Score: 58.2 bits (139), Expect = 4.4e-07
Identity = 72/353 (20.40%), Postives = 152/353 (43.06%), Query Frame = 1

Query: 184 DALNRIRLRIADAVQLYQVSEKAVEQL----EMLLAKSQ---QDIDSLTSQHSYLEKQLS 243
           + LNR+++   +   L     KA+E L    E    K+Q     I  L  +    E Q  
Sbjct: 277 EKLNRVKMVEKEKDALEGEKNKAIEFLTVENETFKKKNQLCQYYIHDLQKRSRDKEAQKE 336

Query: 244 SLEAASKPKDDELNRL----EELRNFILEEEKEISRLVLGSKKLTEKALEIQSQIENAGG 303
            ++  +K   ++ N L    +E    + + EK+++++    ++  EK  ++  Q  +   
Sbjct: 337 KIQEDTKDISEKSNTLLETMKEKNKALKDVEKQLNKITKFIEENREKFTQLDLQDVDTR- 396

Query: 304 ERLKAQKSKVTKIQSDINKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKN 363
           E+LK  KSKV K+Q  + K +  ++  K    + Q  + + T   +  +K+KE+ EE+  
Sbjct: 397 EKLKHSKSKVKKLQKQLQKDKEKVDELKNVPANSQKIIAEETNKKDLLEKQKEKEEEKLK 456

Query: 364 NLQGKFKDIEVKAFAVQENYKETEKLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEY 423
           N+    K  E +    ++  KE E L+ + + V + +++  +  +  +D           
Sbjct: 457 NVMDSLKK-ETQGLQEEKEVKEKE-LMEISKTV-NEARSKMDVAQSELDIYLSRHNSALS 516

Query: 424 KLQDLKKLYKELELKEKGYRTKLDDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVECRD 483
           +L   K+         K  R  + +L+T L K    + K   + E L +       + R+
Sbjct: 517 QLNKAKEALNTASATLKERRAAIKELETKLPKDEGDLKKREKELESLVSEEGNIKNQVRE 576

Query: 484 LKRALEMVTLLDAQLKEMNPNLDSITEYRR--KVEVYNERVEDLNTVTQQRDI 524
           L++ +E      +  +     LD++ + ++  K+     R+ DL  + ++ D+
Sbjct: 577 LRQKVEEARSSLSANRSRGKVLDALIQQKKSGKIPGIFGRLGDLGAIDEKYDV 625

BLAST of Cucsa.011780 vs. Swiss-Prot
Match: SMC4_MOUSE (Structural maintenance of chromosomes protein 4 OS=Mus musculus GN=Smc4 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 8.9e-125
Identity = 279/702 (39.74%), Postives = 423/702 (60.26%), Query Frame = 1

Query: 21   KYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVST 80
            KYD+AIS+ C  LDYIVV++   AQ CV  L++ N+G+ATF+ L+K      K+ +K+ T
Sbjct: 626  KYDIAISSCCHALDYIVVDSIDTAQECVNFLKKHNIGIATFIGLDKMTVWAKKM-SKIQT 685

Query: 81   PEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGALL 140
            PE  PRLFDL+KV+++ ++ AF+ AL +T+VA +L+QATR+AY  +R +R VVTL G ++
Sbjct: 686  PENTPRLFDLVKVKNEEIRQAFYFALRDTLVANNLDQATRVAYQRDRRWR-VVTLQGQII 745

Query: 141  EKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDL---SDMVDALNRIRLRIADAV 200
            E+SGTMSGGG     G+MG+S+    +S E   K E  L   S     +   +++  +AV
Sbjct: 746  EQSGTMSGGGSKVMRGRMGSSVID-EISVEEVNKMESQLERHSKQAMQIQEQKVQHEEAV 805

Query: 201  QLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRN 260
               + SE+    +   L K    I  L+ Q  YL  Q+  LEA       +  + + L  
Sbjct: 806  VKLRHSER---DMRNTLEKFAASIQGLSEQEEYLCVQIKELEANVLTTAPDRKQQKLLEE 865

Query: 261  FILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDIN 320
             +   +KE   +   + K+  +   + + I +    +LKAQ++K+  I   +++  + I 
Sbjct: 866  NVSVFKKEYDAVAEKAGKVEAEIKRLHNTIIDINNRKLKAQQNKLDTINKQLDECASAIT 925

Query: 321  RYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETE- 380
            + +V I++    +KK   ++  ++KE +  E+E N+L+ + K+IE KA  V  N K  E 
Sbjct: 926  KAQVAIKTADRNLKKAQDSVCRTEKEIKDTEKEINDLKTELKNIEDKAEEVINNTKTAET 985

Query: 381  KLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQ----DLKKLYKELELKEKGYR 440
             L  +Q+E            +  + ELK  + + E+ LQ     +K   ++++     + 
Sbjct: 986  SLPEIQKEH-----------RNLLQELKVIQ-ENEHALQKDALSIKLKLEQIDGHISEHN 1045

Query: 441  TKLDDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVEC-RDLKRALEMVTLLDAQLKEMN 500
            +K+   Q  +SK   ++H    +P +  A L+++ +E  ++ +     + LL+AQ +EM 
Sbjct: 1046 SKIKYWQKEISKI--KLHPVEDNPVETVAVLSQEELEAIKNPESITNEIALLEAQCREMK 1105

Query: 501  PNLDSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSS 560
            PNL +I EY++K ++Y +RV +L+ +T +RD  ++ Y++L+K+R  +F + F   T   +
Sbjct: 1106 PNLGAIAEYKKKEDLYLQRVAELDKITSERDNFRQAYEDLRKQRLNEFMAGFYVIT---N 1165

Query: 561  KLILSFYLSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPP 620
            KL  ++                        M+TLGGDAELELVDSLDPFSEG++FSVRPP
Sbjct: 1166 KLKENYQ-----------------------MLTLGGDAELELVDSLDPFSEGIMFSVRPP 1225

Query: 621  KKSWKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRT 680
            KKSWK I NLSGGEKTLSSLALVFALHHYKPTPLY MDEIDAALDFKNVSIV  Y+ ++T
Sbjct: 1226 KKSWKKIFNLSGGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQT 1281

Query: 681  KDAQFIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFS 714
            K+AQFIIISLRNNMFE++DRL+GIYKT N TKS+ +NP+  +
Sbjct: 1286 KNAQFIIISLRNNMFEISDRLIGIYKTYNSTKSVAVNPKQIA 1281

BLAST of Cucsa.011780 vs. Swiss-Prot
Match: SMC4_MICAR (Structural maintenance of chromosomes protein 4 (Fragment) OS=Microtus arvalis GN=SMC4 PE=2 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 4.4e-124
Identity = 275/698 (39.40%), Postives = 413/698 (59.17%), Query Frame = 1

Query: 21   KYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVST 80
            KYD+AIS+ C  LDYIVV++   AQ CV  L+R N+GVATF+ L+K      K+ AK+ T
Sbjct: 583  KYDIAISSCCHALDYIVVDSIDTAQECVNFLKRHNIGVATFIGLDKMTVWAKKM-AKIQT 642

Query: 81   PEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGALL 140
            PE  PRLFDL+K +++ ++ AF+ AL +T+VA +L+QATR+AY  +R +R VVTL G ++
Sbjct: 643  PENTPRLFDLVKAKNEEIRQAFYFALRDTLVADNLDQATRVAYQKDRRWR-VVTLQGQII 702

Query: 141  EKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQLY 200
            E+SGTM+GGG     G+MG+S+    +S+E   K E  L        RI+ +     +  
Sbjct: 703  EQSGTMTGGGSKVMRGRMGSSV-IVEISEEEVNKMESQLQKHSKQARRIQEQKVQHEERV 762

Query: 201  QVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFIL 260
                 +  ++   L K    I  L+ Q  YL  Q+  LEA       +  + + L   + 
Sbjct: 763  VKLRHSEREMRNTLEKFAASIQGLSDQEEYLTVQIKELEANVLTTAPDKKKQKLLEENVS 822

Query: 261  EEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRYK 320
              +KE   +   + K+  +   +   I      +LKAQ++K+  I   +++  + I + +
Sbjct: 823  AFKKEYDAVAEKAGKVEAEVKRLHDTIIEINNRKLKAQQNKLDMINKQLDECASAITKAQ 882

Query: 321  VQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLIH 380
            V I++    + K   ++  ++KE +  E+E N+L+ + K IE KA   +E  K+T     
Sbjct: 883  VAIKTADRNLIKAQDSVVRTEKEIKDTEKETNDLKAELKAIEDKA---EEVIKKTNAA-- 942

Query: 381  LQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQ----DLKKLYKELELKEKGYRTKLD 440
             +E + +  K + N     + ELK  + + E+ LQ     +K   ++++     + +K+ 
Sbjct: 943  -EESLPEIQKEHRN----LLQELKVIQ-ENEHALQKDALSIKLKLEQIDGHIAEHNSKIK 1002

Query: 441  DLQTALSKHMDQIHKDLVDPEKLQATLA-EDIVECRDLKRALEMVTLLDAQLKEMNPNLD 500
              Q  +SK   ++H    +P +  + L+ ED+   ++       + +L+AQ  EM PNL 
Sbjct: 1003 YWQKEISKI--KLHPIEDNPVETVSVLSPEDLEAIKNPDSITNQIAILEAQCHEMKPNLG 1062

Query: 501  SITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLIL 560
            +I EY++K E+Y +RV +L+ +T +RD  ++ Y++L+K+R  +F + F   T   +KL  
Sbjct: 1063 AIAEYKKKEELYLQRVAELDKITSERDNFRQAYEDLRKQRLNEFMAGFYIIT---NKLKE 1122

Query: 561  SFYLSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSW 620
            ++                        M+TLGGDAELELVDSLDPFSEG++FSVRPPKKSW
Sbjct: 1123 NYQ-----------------------MLTLGGDAELELVDSLDPFSEGIMFSVRPPKKSW 1182

Query: 621  KNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQ 680
            K I NLSGGEKTLSSLALVFALHHYKPTPLY MDEIDAALDFKNVSIV  Y+ ++TK+AQ
Sbjct: 1183 KKIFNLSGGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQ 1238

Query: 681  FIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFS 714
            FIIISLRNNMFE++DRL+GIYKT N TKS+ +NP+  +
Sbjct: 1243 FIIISLRNNMFEISDRLIGIYKTYNITKSVAVNPKEIA 1238

BLAST of Cucsa.011780 vs. TrEMBL
Match: A0A0A0KSL0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G533470 PE=4 SV=1)

HSP 1 Score: 1250.3 bits (3234), Expect = 0.0e+00
Identity = 661/714 (92.58%), Postives = 670/714 (93.84%), Query Frame = 1

Query: 6   QELTTDIVTQNSNFAKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILE 65
           + + + + +  S  AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILE
Sbjct: 20  ENICSFVASTVSTEAKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILE 79

Query: 66  KQVDHLSKLKAKVSTPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGG 125
           KQVDHLSKLKAKVSTPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGG
Sbjct: 80  KQVDHLSKLKAKVSTPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGG 139

Query: 126 NRDFRRVVTLDGALLEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDA 185
           NRDFRRVVTLDGALLEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDA
Sbjct: 140 NRDFRRVVTLDGALLEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDA 199

Query: 186 LNRIRLRIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPK 245
           LNRIRLRIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPK
Sbjct: 200 LNRIRLRIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPK 259

Query: 246 DDELNRLEELRNFILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKI 305
           DDELNRLEELRNFILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKI
Sbjct: 260 DDELNRLEELRNFILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKI 319

Query: 306 QSDINKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKA 365
           QSDINKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKA
Sbjct: 320 QSDINKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKA 379

Query: 366 FAVQENYKETEKLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELE 425
           FAVQENYKETEKLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELE
Sbjct: 380 FAVQENYKETEKLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELE 439

Query: 426 LKEKGYRTKLDDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDA 485
           LKEKGYRTKLDDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDA
Sbjct: 440 LKEKGYRTKLDDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDA 499

Query: 486 QLKEMNPNLDSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKK 545
           QLKEMNPNLDSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKR  +F S F  
Sbjct: 500 QLKEMNPNLDSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRLDEFMSGFNT 559

Query: 546 CTRFSSKLILSFYLSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVV 605
                        +SL    M++             MITLGGDAELELVDSLDPFSEGVV
Sbjct: 560 -------------ISLKLKEMYQ-------------MITLGGDAELELVDSLDPFSEGVV 619

Query: 606 FSVRPPKKSWKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGH 665
           FSVRPPKKSWKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGH
Sbjct: 620 FSVRPPKKSWKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGH 679

Query: 666 YVKDRTKDAQFIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
           YVKDRTKDAQFIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA
Sbjct: 680 YVKDRTKDAQFIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 707

BLAST of Cucsa.011780 vs. TrEMBL
Match: B9IJY0_POPTR (Structural maintenance of chromosomes protein OS=Populus trichocarpa GN=POPTR_0017s11950g PE=3 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 4.6e-285
Identity = 520/701 (74.18%), Postives = 587/701 (83.74%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTACPGLDYIVVET+ AAQACVELLRRE LGVATFMILEKQVDH SK+K  VS
Sbjct: 576  AKYDVAISTACPGLDYIVVETTAAAQACVELLRREKLGVATFMILEKQVDHSSKMKHNVS 635

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPEGVPRLFDL++VQD+RMKLAF+AALGNTVVAKDL+QATRIAYGGN +FRRVVTLDGAL
Sbjct: 636  TPEGVPRLFDLVRVQDERMKLAFYAALGNTVVAKDLDQATRIAYGGNLEFRRVVTLDGAL 695

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
             EKSGTMSGGG  PRGGKMGTSIR+ SVS EA   AEK+LS MVD LN IR RIAD+V+ 
Sbjct: 696  FEKSGTMSGGGTKPRGGKMGTSIRATSVSGEAVTSAEKELSTMVDRLNGIRQRIADSVKH 755

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            YQ SEKA+  LEM LAKSQ++IDSL ++HSYLEKQL SL+AAS+PK DEL+RLEEL+  I
Sbjct: 756  YQASEKAIAHLEMELAKSQKEIDSLNTEHSYLEKQLGSLKAASEPKKDELDRLEELKRII 815

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
            + EEKEI RL+ GSKKL EKALE+QS+IENAGGERLK+QK+KV +IQSD++K  T+INR+
Sbjct: 816  VTEEKEIDRLIQGSKKLKEKALELQSKIENAGGERLKSQKAKVNRIQSDMDKNSTEINRH 875

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
            KVQIE+    +KKLTK IEDS+KEKERL EE+  L+G FK+IE KAFAVQENYK+T++LI
Sbjct: 876  KVQIETGHKMIKKLTKGIEDSRKEKERLTEEREKLRGIFKEIEEKAFAVQENYKKTQELI 935

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
               +EV D +K+ Y KVKK +DEL+ SEVD +Y+LQD+KK YKELELK KGY+ KLDDLQ
Sbjct: 936  DQHKEVLDKAKSEYEKVKKIVDELRASEVDADYRLQDMKKSYKELELKGKGYKKKLDDLQ 995

Query: 440  TALSKHMDQIHKDLVDPEKLQATLAE-DIVECRDLKRALEMVTLLDAQLKEMNPNLDSIT 499
             AL  HM+Q  K+L DPEKLQATLA+  + E  DLKRALE V LL+AQLK+MNPNLDSI+
Sbjct: 996  NALLHHMEQTQKELEDPEKLQATLADKTLAEACDLKRALERVVLLEAQLKDMNPNLDSIS 1055

Query: 500  EYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFY 559
            EYRRKV  YNERVE+LN VTQQRD +K+QYDE +KKR                 L+ SF 
Sbjct: 1056 EYRRKVSSYNERVEELNLVTQQRDDIKRQYDEWRKKR-----------------LVFSFL 1115

Query: 560  LSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 619
            L     FM   +T  + L+    MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI
Sbjct: 1116 LD---EFMAGFNTISLKLKEMYQMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 1175

Query: 620  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 679
            ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII
Sbjct: 1176 ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 1235

Query: 680  ISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
            ISLRNNMFELADRLVGIYKT+NCTKSITINP SF VC+  A
Sbjct: 1236 ISLRNNMFELADRLVGIYKTDNCTKSITINPGSFVVCQNAA 1256

BLAST of Cucsa.011780 vs. TrEMBL
Match: B9T849_RICCO (Structural maintenance of chromosomes protein OS=Ricinus communis GN=RCOM_0100760 PE=3 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 2.1e-282
Identity = 519/701 (74.04%), Postives = 585/701 (83.45%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTACPGLDYIVVET+ AAQACVELLRRENLGVATFMILEKQVD L KLKAKV+
Sbjct: 571  AKYDVAISTACPGLDYIVVETTAAAQACVELLRRENLGVATFMILEKQVDLLPKLKAKVT 630

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            +PEGVPRLFDL+KVQD+RMKLAF+AALGNTVVA DL+QATRIAYG N DFRRVVTLDGAL
Sbjct: 631  SPEGVPRLFDLVKVQDERMKLAFYAALGNTVVASDLDQATRIAYGRNMDFRRVVTLDGAL 690

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
             EKSGTMSGGG  PRGGKMGTSIRSASVS E    AEK+LS MV  LN IR +I DAV+ 
Sbjct: 691  FEKSGTMSGGGSKPRGGKMGTSIRSASVSAEVVANAEKELSTMVGKLNDIRQKIIDAVRS 750

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            YQ SEKA+  +EM LAKSQ++IDSL S+HSYLEKQL SLEAAS+PK DEL+RL+EL+  I
Sbjct: 751  YQASEKAITHMEMELAKSQKEIDSLNSEHSYLEKQLGSLEAASQPKKDELDRLKELKKII 810

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
              EE EI RL  GSKKL EKALE+Q++IENAGGE LKAQK+KV KIQS+I+KT T+INR 
Sbjct: 811  SSEEMEIDRLTQGSKKLKEKALELQNKIENAGGETLKAQKAKVKKIQSEIDKTSTEINRQ 870

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
            KVQIE++Q  +KKLTK IEDSKKEK+R  EEK  L+  FK+IE KAFAVQENYK+T++LI
Sbjct: 871  KVQIETNQKMIKKLTKGIEDSKKEKDRFVEEKEKLKSVFKEIEEKAFAVQENYKKTQQLI 930

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
               +EV D +K+ Y  VKK +DEL+ SEVD +YKLQD+KK YKELELK KGY+ KLDDLQ
Sbjct: 931  DQHKEVLDKAKSEYENVKKIVDELRASEVDADYKLQDMKKCYKELELKGKGYKKKLDDLQ 990

Query: 440  TALSKHMDQIHKDLVDPEKLQATLA-EDIVECRDLKRALEMVTLLDAQLKEMNPNLDSIT 499
             AL+ HM+QI KDLVDPEKLQATLA E + +  DL+RA+E V LL+AQLKEMNPNL+SI+
Sbjct: 991  NALTHHMEQIQKDLVDPEKLQATLADETLAKACDLRRAMETVALLEAQLKEMNPNLESIS 1050

Query: 500  EYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFY 559
            EYR KV +YN RVE+LNTVTQQRD +KKQ+DE +KKRQ         C  ++  ++L   
Sbjct: 1051 EYRGKVSLYNGRVEELNTVTQQRDDIKKQHDEWRKKRQ-------DYCHLYTLVMLL--- 1110

Query: 560  LSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 619
              +   FM   +T  + L+    MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI
Sbjct: 1111 --MLDEFMAGFNTISLKLKEMYQMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 1170

Query: 620  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 679
            ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII
Sbjct: 1171 ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 1230

Query: 680  ISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
            ISLRNNMFELADRLVGIYKT+NCTKSITINP SF VC   A
Sbjct: 1231 ISLRNNMFELADRLVGIYKTDNCTKSITINPGSFVVCGNAA 1259

BLAST of Cucsa.011780 vs. TrEMBL
Match: A0A0B0MUD9_GOSAR (Structural maintenance of chromosomes protein OS=Gossypium arboreum GN=F383_05923 PE=3 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 2.8e-282
Identity = 510/701 (72.75%), Postives = 586/701 (83.59%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTACPGLDYIVVET+ AAQACVELLRRE LG+ATFMILEKQ+D L + K KV 
Sbjct: 571  AKYDVAISTACPGLDYIVVETTAAAQACVELLRREQLGIATFMILEKQLDLLERSKEKVR 630

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPEGVPRL+DLIKVQD+R+KLAF+AALGNT+VAKDL+QATRIAYGGN++FRRVVTLDGAL
Sbjct: 631  TPEGVPRLYDLIKVQDERIKLAFYAALGNTIVAKDLDQATRIAYGGNKEFRRVVTLDGAL 690

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
             EKSGTMSGGG  PRGGKMGTSIR+ASVS+E  V AEK+L++MVD+LN IR RIADA + 
Sbjct: 691  FEKSGTMSGGGSKPRGGKMGTSIRAASVSRETVVAAEKELANMVDSLNNIRQRIADAARR 750

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            YQ SEK V +LEM +AKSQ+++DSL S++ YLEKQL SLEAAS+PK DE++RLEEL+  I
Sbjct: 751  YQASEKVVAELEMEIAKSQKEVDSLNSEYKYLEKQLDSLEAASRPKKDEIDRLEELKKII 810

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
              EEKEI RL+ GSKKL EKA ++Q++IENAGGE+LK QKSKV KIQSDI+K  T+INR+
Sbjct: 811  STEEKEIDRLIQGSKKLKEKASDLQNKIENAGGEKLKTQKSKVEKIQSDIDKNSTEINRH 870

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
            KVQIE+ +  +KKLTK IE+SKKEKER+ E K+ + G FK+IE KAF VQ+NYK+ +K+I
Sbjct: 871  KVQIETGEKMVKKLTKGIEESKKEKERIIEGKDKMHGMFKEIEQKAFIVQDNYKKMQKVI 930

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
                EV + SK  Y KVKK +D+L+ SEVD ++KLQD+KK+YKELE+K KGY+ KL+DL+
Sbjct: 931  DEHGEVLEKSKLEYEKVKKNVDQLRASEVDADFKLQDMKKMYKELEMKGKGYKKKLNDLE 990

Query: 440  TALSKHMDQIHKDLVDPEKLQATLA-EDIVECRDLKRALEMVTLLDAQLKEMNPNLDSIT 499
             +L KHM+QI KDLVD EKLQATLA E + E  DLKRALEMVTLL+ QLKEMNPNLDSI+
Sbjct: 991  ISLQKHMEQIQKDLVDTEKLQATLADETLTEACDLKRALEMVTLLETQLKEMNPNLDSIS 1050

Query: 500  EYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFY 559
            EYR KV VYNERVEDLNTVTQQRD +KKQYDEL+KKR  +F + F               
Sbjct: 1051 EYRNKVSVYNERVEDLNTVTQQRDDIKKQYDELRKKRLDEFMAGFNA------------- 1110

Query: 560  LSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 619
            +SL    M++             MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI
Sbjct: 1111 ISLKLKEMYQ-------------MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 1170

Query: 620  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 679
            ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII
Sbjct: 1171 ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 1230

Query: 680  ISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
            ISLRNNMFELADRLVGIYKT+NCTKSITINP SF VCEK A
Sbjct: 1231 ISLRNNMFELADRLVGIYKTDNCTKSITINPNSFVVCEKAA 1245

BLAST of Cucsa.011780 vs. TrEMBL
Match: A0A0B0MUD9_GOSAR (Structural maintenance of chromosomes protein OS=Gossypium arboreum GN=F383_05923 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.7e-03
Identity = 89/394 (22.59%), Postives = 172/394 (43.65%), Query Frame = 1

Query: 167 VSKEAFVKAEKDLSDMVDALNRIRLRIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTS 226
           +    +V+   + S  +++LN  R   +  VQ+ +++EK  + LE          D    
Sbjct: 202 IGTNKYVEKIDESSKELESLNEKR---SGVVQMVKLAEKERDSLE----------DVKNE 261

Query: 227 QHSYLEKQLSSL---EAASKPKDDELN-RLEELRNFI--LEEEKEISRLVL--GSKKLTE 286
             +Y+ K+LS L   E A+K   ++ N ++ ELR  +  LEE  +  R  +   SK+L E
Sbjct: 262 AEAYMLKELSLLKWQEKAAKLAHEDTNLKMVELRENVSNLEENLKNKREEIQESSKRLKE 321

Query: 287 ------KALEIQSQIEN---AGGERLKAQKSKVTKIQSDINKTRTDINRYKVQIESDQAT 346
                 K L  + +++N      E  K  + +  K + D+   +  + + + ++E D + 
Sbjct: 322 IESVHNKHLIRKEELDNDLRTCKEEFKEFERQDVKYREDLKHMKQKLKKLEDKLEKDSSK 381

Query: 347 MKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYK-ETEK----LIHLQEE 406
           ++ ++K  E+SK    +LEE    LQ    D E     ++EN K ETEK    L  ++ E
Sbjct: 382 IEDVSKECENSKNLIPKLEENIPKLQKLLLDEEKLLEEMKENSKVETEKYRSELSKVRAE 441

Query: 407 --------VCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLD 466
                   +    K      ++ +   K    +T +K  D +K    +  K +     + 
Sbjct: 442 LEPWEKELIVHKGKLEVAHTERNLLTQKHEAANTAFK--DAQKEMDNISGKTETITAAIK 501

Query: 467 DLQTALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRA-----LEMVTLLDAQLKEMN 524
           D QT L K+        ++  KL+ T  ++      L++A      E+ ++LD++ K   
Sbjct: 502 DKQTDLEKN----KLGALEARKLEQTCIKEQEALIPLEQAAREKFAELKSVLDSE-KSQG 561

HSP 2 Score: 977.6 bits (2526), Expect = 8.0e-282
Identity = 512/701 (73.04%), Postives = 586/701 (83.59%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTACPGL+YIVVET+GAAQACVELLRR+NLGVATFMILEKQVDHL ++K KVS
Sbjct: 573  AKYDVAISTACPGLEYIVVETTGAAQACVELLRRKNLGVATFMILEKQVDHLHRMKDKVS 632

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPEGVPRLFDLIK+QD+RMKLAFFAALGNTVVAKD++QATRIAYGGN++FRRVVTL+GAL
Sbjct: 633  TPEGVPRLFDLIKIQDERMKLAFFAALGNTVVAKDIDQATRIAYGGNKEFRRVVTLEGAL 692

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
             EKSGTMSGGG  PRGG+MGTSIR ASVS E+   A+ +LS MVD LN +R ++ DAV++
Sbjct: 693  FEKSGTMSGGGGKPRGGRMGTSIRPASVSAESVATAKNELSAMVDKLNSMRQKVVDAVRI 752

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            YQ SEKAV +LEM L K  ++IDSL SQHSYLEKQL SL+AASKP+ DELNRLE L   I
Sbjct: 753  YQASEKAVARLEMELTKIHKEIDSLKSQHSYLEKQLDSLKAASKPRKDELNRLEVLNKTI 812

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
              E+KEI RL+ GSK+L +KALE+QS+IENAGGERLK QKSKV KIQ DI+K+ T+INR+
Sbjct: 813  SAEKKEIERLIEGSKQLKDKALELQSKIENAGGERLKLQKSKVNKIQFDIDKSNTEINRH 872

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
            KVQIE+ Q  MKKL K IE+SKKEK+R+ +EK  L    KDIE KAF+VQ+NY +T++LI
Sbjct: 873  KVQIETGQKMMKKLKKGIEESKKEKDRVVDEKEKLHLSHKDIEQKAFSVQDNYNKTQELI 932

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
               ++V D +K++Y K+KKT+DEL+ SEVD +YKLQD+KKLYKELE+K KGY+ KL++LQ
Sbjct: 933  DQHKDVLDKAKSDYEKLKKTVDELRASEVDVDYKLQDMKKLYKELEMKGKGYKRKLEELQ 992

Query: 440  TALSKHMDQIHKDLVDPEKLQATLAE-DIVECRDLKRALEMVTLLDAQLKEMNPNLDSIT 499
             AL KHM+QI KDLVDPEKLQATLA+  + E   LKRALEMV L++AQLKEMNPNLDSI+
Sbjct: 993  VALVKHMEQIQKDLVDPEKLQATLADKTLTEDCGLKRALEMVALIEAQLKEMNPNLDSIS 1052

Query: 500  EYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFY 559
            EYRRKV VYNERV+DLN VTQ+RD VKKQYDE KK+R +                    Y
Sbjct: 1053 EYRRKVSVYNERVQDLNMVTQERDDVKKQYDEWKKRRLV--------------------Y 1112

Query: 560  LSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 619
             +L   FM    T  + L+    MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI
Sbjct: 1113 FTLMDEFMAGFHTISLKLKEMYQMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 1172

Query: 620  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 679
            ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII
Sbjct: 1173 ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 1232

Query: 680  ISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
            ISLRNNMFELADRLVGIYKT+NCTKSITINP SF VCEK A
Sbjct: 1233 ISLRNNMFELADRLVGIYKTDNCTKSITINPGSFVVCEKAA 1253

BLAST of Cucsa.011780 vs. TAIR10
Match: AT5G48600.2 (AT5G48600.2 structural maintenance of chromosome 3)

HSP 1 Score: 923.7 bits (2386), Expect = 7.0e-269
Identity = 480/699 (68.67%), Postives = 571/699 (81.69%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTAC GLDYIVVET+ +AQACVELLR+ NLG ATFMILEKQ DH+ KLK KV 
Sbjct: 569  AKYDVAISTACAGLDYIVVETTSSAQACVELLRKGNLGFATFMILEKQTDHIHKLKEKVK 628

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPE VPRLFDL++V+D+RMKLAF+AALGNTVVAKDL+QATRIAYGGNR+FRRVV LDGAL
Sbjct: 629  TPEDVPRLFDLVRVKDERMKLAFYAALGNTVVAKDLDQATRIAYGGNREFRRVVALDGAL 688

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
             EKSGTMSGGG   RGG+MGTSIR+  VS EA   AE +LS +VD LN IR ++ +AV+ 
Sbjct: 689  FEKSGTMSGGGGKARGGRMGTSIRATGVSGEAVANAENELSKIVDMLNNIREKVGNAVRQ 748

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            Y+ +E  V  LEM LAKSQ++I+SL S+H+YLEKQL+SLEAAS+PK DE++RL+EL+  I
Sbjct: 749  YRAAENEVSGLEMELAKSQREIESLNSEHNYLEKQLASLEAASQPKTDEIDRLKELKKII 808

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
             +EEKEI  L  GSK+L +KALE+Q+ IENAGGE+LK QK+KV KIQ+DI+K  T+INR 
Sbjct: 809  SKEEKEIENLEKGSKQLKDKALELQTNIENAGGEKLKGQKAKVEKIQTDIDKNNTEINRC 868

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
             VQIE++Q  +KKLTK IE++ +EKERLE EK NL   FKDI  KAF +QE YK+T++LI
Sbjct: 869  NVQIETNQKLIKKLTKGIEEATREKERLEGEKENLHVTFKDITQKAFEIQETYKKTQQLI 928

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
               ++V   +K++Y  +KK++DELK S VD E+K+QD+KK Y ELE++EKGY+ KL+DLQ
Sbjct: 929  DEHKDVLTGAKSDYENLKKSVDELKASRVDAEFKVQDMKKKYNELEMREKGYKKKLNDLQ 988

Query: 440  TALSKHMDQIHKDLVDPEKLQATLAE-DIVECRDLKRALEMVTLLDAQLKEMNPNLDSIT 499
             A +KHM+QI KDLVDP+KLQATL + ++ E  DLKRALEMV LL+AQLKE+NPNLDSI 
Sbjct: 989  IAFTKHMEQIQKDLVDPDKLQATLMDNNLNEACDLKRALEMVALLEAQLKELNPNLDSIA 1048

Query: 500  EYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFY 559
            EYR KVE+YN RV++LN+VTQ+RD  +KQYDEL+K+R  +F + F               
Sbjct: 1049 EYRSKVELYNGRVDELNSVTQERDDTRKQYDELRKRRLDEFMAGFNT------------- 1108

Query: 560  LSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 619
            +SL    M++             MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI
Sbjct: 1109 ISLKLKEMYQ-------------MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 1168

Query: 620  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 679
            ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII
Sbjct: 1169 ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 1228

Query: 680  ISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEK 718
            ISLRNNMFELADRLVGIYKT+NCTKSITINP SF+VC+K
Sbjct: 1229 ISLRNNMFELADRLVGIYKTDNCTKSITINPGSFAVCQK 1241

HSP 2 Score: 58.9 bits (141), Expect = 1.5e-08
Identity = 83/382 (21.73%), Postives = 167/382 (43.72%), Query Frame = 1

Query: 204 EKAVEQLEMLLAKSQ--QDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFILE 263
           E  +E LE ++  ++  + ID L  Q   L +  S +    K  + E + LE L++   E
Sbjct: 190 EGFLEYLEDIIGTNKYVEKIDELNKQLETLNESRSGVVQMVKLAEKERDNLEGLKD---E 249

Query: 264 EE----KEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDIN 323
            E    KE+S L     K  EKA ++  +   A          K+T+ +  +      + 
Sbjct: 250 AETYMLKELSHL-----KWQEKATKMAYEDTVA----------KITEQRDSLQNLENSLK 309

Query: 324 RYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEK 383
             +V+++     +KK     E  KK +E L+ E    + KFK+ E +    +E+ K  ++
Sbjct: 310 DERVKMDESNEELKKFESVHEKHKKRQEVLDNELRACKEKFKEFERQDVKHREDLKHVKQ 369

Query: 384 LIHLQEE--------VCDTSKANYN------KVKKTMDELKGSEVDTEYKLQDLKKLYKE 443
            I   E+        + D +K + +      K+++ + +L+   +D E KL+++K + K 
Sbjct: 370 KIKKLEDKLEKDSSKIGDMTKESEDSSNLIPKLQENIPKLQKVLLDEEKKLEEIKAIAK- 429

Query: 444 LELKEKGYRTKLDDLQTALSKHMDQIHKDL-VDPEKLQATLAEDIVECRDLKRALEMVTL 503
             ++ +GYR++L    T +   ++   KDL V   KL    +E  +  +  + AL+  T 
Sbjct: 430 --VETEGYRSEL----TKIRAELEPWEKDLIVHRGKLDVASSESELLSKKHEAALKAFTD 489

Query: 504 LDAQLKEMN----PNLDSITEY-----RRKVEVYNERVEDLNTVTQQRDIVKKQYDELKK 556
              QL +++        + T +     ++K E    R  +  ++ +Q  +V ++    +K
Sbjct: 490 AQKQLSDISTRKKEKAAATTSWKADIKKKKQEAIEARKVEEESLKEQETLVPQEQAAREK 546

BLAST of Cucsa.011780 vs. TAIR10
Match: AT3G54670.3 (AT3G54670.3 Structural maintenance of chromosomes (SMC) family protein)

HSP 1 Score: 186.4 bits (472), Expect = 6.1e-47
Identity = 175/759 (23.06%), Postives = 339/759 (44.66%), Query Frame = 1

Query: 18   NFAKYDVAISTACPG-LDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKA 77
            N  KY++A++ A    +D +VVE     + C++ L+ + L   TF+ L+     + ++  
Sbjct: 532  NRKKYNLAVTVAMGRFMDAVVVEDENTGKDCIKYLKEQRLPPMTFIPLQSV--RVKQVFE 591

Query: 78   KVSTPEGVPRL-FDLIKVQ-------------------DDRMKLAFFAALGNTVVAKDLE 137
            ++    G  +L FD+I+                     D  ++ A   A+GNT+V  +LE
Sbjct: 592  RLRNLGGTAKLVFDVIQYPLHISEVSKIYIFVLNYSTFDPELEKAVLYAVGNTLVCDELE 651

Query: 138  QATRIAYGGNRDFRRVVTLDGALLEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAE 197
            +A  +++ G R   +VVT+DG LL K+GTM+GG     GG    S +      E   K +
Sbjct: 652  EAKVLSWSGERF--KVVTVDGILLTKAGTMTGG---TSGGMEAKSNKWDDKKIEGLKKNK 711

Query: 198  KDLSDMVDALNRIRL----------RIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTS 257
            +D    ++ +  IR           +I+   +  Q +E   + ++  L + +Q+  ++  
Sbjct: 712  EDFEQQLENIGSIREMQMKESEISGKISGLEKKIQYAEIEKKSIKDKLPQLEQEERNIIE 771

Query: 258  QHSYLEKQLSSLEAASKPKDDELNRLEELRNFILEE-EKEISRLV-------------LG 317
            +   ++ +LS        +  E+N+LE+  N I++   K+ S+ V               
Sbjct: 772  EIDRIKPELSKARTEVDKRKTEMNKLEKRMNEIVDRIYKDFSQSVGVPNIRVYEETQLKT 831

Query: 318  SKKLTEKALEIQSQIENAGGERLKAQK----SKVTKIQSDINKTRTDINRYKVQIESDQA 377
            ++K  E+ LE+ +Q+     +    Q     S++ KI+S I+   TD+   +  +   + 
Sbjct: 832  AEKEAEERLELSNQLAKLKYQLEYEQNRDVGSRIRKIESSISSLETDLEGIQKTMSERKE 891

Query: 378  TMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLIHLQEEVCDT 437
            T  K+T  I + KKE E  +++    + +  D + +A     +  +  + IH +E    T
Sbjct: 892  TAVKITNEINNWKKEMEECKQKSEEYEKEILDWKKQASQATTSITKLNRQIHSKE----T 951

Query: 438  SKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQTALSKHMDQ 497
                    K+ + E    E  T   L D  +     E    G +    +L  A  +    
Sbjct: 952  QIEQLISQKQEITEKCELEHITLPVLSDAME-----EDDSDGPQFDFSELGRAYLQERRP 1011

Query: 498  IHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDAQLKEMNPNLDSITEYRRKVEVYN 557
              ++ V+ E                ++ +E  T   ++++   PNL ++ +Y    E   
Sbjct: 1012 SAREKVEAE---------------FRQKIESKT---SEIERTAPNLRALDQYEAIQEKEK 1071

Query: 558  ERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFYLSLTFTFMFE 617
            +  ++     ++   V   ++ +K+KR   F   F        K+      S T      
Sbjct: 1072 QVSQEFEAARKEEKQVADAFNTVKQKRYELFMEAFNHIASNIDKIYKQLTKSNTHP---- 1131

Query: 618  CSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNIANLSGGEKTL 677
                            LGG A L L +  DPF  G+ ++  PP K ++++  LSGGEKT+
Sbjct: 1132 ----------------LGGTAYLNLENEDDPFLHGIKYTTMPPTKRFRDMEQLSGGEKTV 1191

Query: 678  SSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDA-------------Q 713
            ++LAL+F++H Y+P+P +++DE+DAALD  NV+ V  +++ ++  A             Q
Sbjct: 1192 AALALLFSIHSYRPSPFFILDEVDAALDNLNVAKVAKFIRSKSCQAARDNQDAEDGNGFQ 1236

BLAST of Cucsa.011780 vs. TAIR10
Match: AT5G62410.1 (AT5G62410.1 structural maintenance of chromosomes 2)

HSP 1 Score: 132.1 bits (331), Expect = 1.4e-30
Identity = 143/614 (23.29%), Postives = 264/614 (43.00%), Query Frame = 1

Query: 96   DRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRV-VTLDGALLEKSGTMSGGGRMPR 155
            D +K A     G+T V K  + A  +A+  NRD R   VTL+G + + SG ++GG R   
Sbjct: 612  DELKNAMEYVFGSTFVCKTTDVAKEVAF--NRDIRTPSVTLEGDIFQPSGLLTGGSRKGG 671

Query: 156  GGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQLYQVSEKAVEQLEMLL 215
            G ++   +   + ++      +K L+D+   +  ++        +Y   E     L + L
Sbjct: 672  GDRL-RKLHDLAEAESELQGHQKRLADVESQIKELQPLQMKFTDVYAQLELKTYDLSLFL 731

Query: 216  AKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFILEEEKEISRLVLGSK 275
             +++Q+      +H  L + +  LE            LEE ++ I  +EKE++       
Sbjct: 732  KRAEQN------EHHKLGEAVKKLE----------EELEEAKSQI--KEKELAY------ 791

Query: 276  KLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRYKVQIESDQATMKKLT 335
               +   +  S++EN+  +  K ++ ++  ++ +I   +  +      ++S +   +KL 
Sbjct: 792  ---KNCFDAVSKLENSIKDHDKNREGRLKDLEKNIKTIKAQMQAASKDLKSHENEKEKLV 851

Query: 336  KAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLIHLQEEVCDTSKANYN 395
               E  K+E+  LE    +L+ +   +  +   V E   + + L  + +E     K  + 
Sbjct: 852  MEEEAMKQEQSSLESHLTSLETQISTLTSE---VDEQRAKVDALQKIHDESLAELKLIHA 911

Query: 396  KVKKTMDELKGSEVDTE---YKLQDLKKLYKELELKEKGYRTKLDDLQTALSKHMDQIHK 455
            K+K+   ++ G   D E    KL D+K   K+LE +     T   D    + K +++ H 
Sbjct: 912  KMKECDTQISGFVTDQEKCLQKLSDMKLERKKLENEVVRMETDHKDCSVKVDKLVEK-HT 971

Query: 456  DLVDPEKLQATLAEDI-VECRDLKRALEMVTLLDAQLK--EMNPNLDSITEYRRKVEVYN 515
             +   ++L      D   E  D   A E +  L +     E   N   +  + +  + YN
Sbjct: 972  WIASEKQLFGKGGTDYDFESCDPYVAREKLEKLQSDQSGLEKRVNKKVMAMFEKAEDEYN 1031

Query: 516  ERVEDLNTVTQQRDIVKKQYDEL--KKKRQMKFSSLFKKCTRFSSKLILSFYLSLTFTFM 575
              +   NT+   +  + K  +EL  KKK  +K                            
Sbjct: 1032 ALISKKNTIENDKSKITKVIEELDEKKKETLK---------------------------- 1091

Query: 576  FECSTTWIVL--EYFEYMITL--GGDAELELVDS---LDPFSEGVVFSVRPPKKSWK-NI 635
                 TW+ +  ++     TL  G  A+LE  +    LD     V F      K WK ++
Sbjct: 1092 ----VTWVKVNQDFGSIFSTLLPGTMAKLEPPEDGNFLDGLEVRVAFG-----KVWKQSL 1151

Query: 636  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 693
            + LSGG+++L +L+L+ AL  +KP PLY++DE+DAALD  +   +G  ++     +QFI+
Sbjct: 1152 SELSGGQRSLLALSLILALLLFKPAPLYILDEVDAALDLSHTQNIGRMIRAHFPHSQFIV 1154

BLAST of Cucsa.011780 vs. TAIR10
Match: AT3G47460.1 (AT3G47460.1 Structural maintenance of chromosomes (SMC) family protein)

HSP 1 Score: 123.2 bits (308), Expect = 6.3e-28
Identity = 163/730 (22.33%), Postives = 303/730 (41.51%), Query Frame = 1

Query: 1    MKGIIQELTTDIVTQNSNFAKYDVAISTACPGLDYIVVETSGAAQACVEL--LRRENLGV 60
            +KG++ +L    V   S+    +V   TA   L  ++V+T    +  ++   LRR     
Sbjct: 520  VKGVVAKLIK--VNDRSSMTALEV---TAGGKLFNVIVDTEDTGKQLLQKGDLRRR---- 579

Query: 61   ATFMILEKQVDHLSKLKAKVSTP-EGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQ 120
             T + L K   HL   + + +T  +G   L   +    + +K A     G+T V K  + 
Sbjct: 580  VTIIPLNKIQSHLVPPRVQQATVGKGNAELALSLVGYSEELKNAMEYVFGSTFVCKTTDA 639

Query: 121  ATRIAYGGNRDFRRV-VTLDGALLEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAE 180
            A  +A+  NR+ R   VTL+G + + SG ++GG R   G  +                  
Sbjct: 640  AKEVAF--NREIRTPSVTLEGDVFQPSGLLTGGSRKGGGDLL------------------ 699

Query: 181  KDLSDMVDALNRIRLRIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLS 240
            + L D+ +A  + R             +K++ ++E  + + Q      T   + LE ++ 
Sbjct: 700  RQLHDLAEAETKFRAH-----------QKSLSEIEANIKELQPLQTKFTDMKAQLELKMY 759

Query: 241  SLEAASK-PKDDELNRLEELRNFILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERL 300
             +    K  + +E ++L +    + EE +E+   +   + L +   +  S +E +  +  
Sbjct: 760  DMSLFLKRAEQNEHHKLGDAVKKLEEEVEEMRSQIKEKEGLYKSCADTVSTLEKSIKDHD 819

Query: 301  KAQKSKVTKIQSDINKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQ 360
            K ++ ++  ++ +I   +  I      ++  +   ++L    E   +E+  L+ +  +L+
Sbjct: 820  KNREGRLKDLEKNIKTLKARIQASSKDLKGHENVRERLVMEQEAVTQEQSYLKSQLTSLR 879

Query: 361  GKFKDI-------EVKAFAVQENYKETE---KLIHLQEEVCDTSKANYNKVKKTMDELKG 420
             +   +         K  A+Q+++ ++    KLIH + + CDT             ++ G
Sbjct: 880  TQISTLASDVGNQRAKVDAIQKDHDQSLSELKLIHAKMKECDT-------------QISG 939

Query: 421  SEVDTEYKLQ-------DLKKLYKE---LELKEKGYRTKLDDLQTALSKHMDQIHKDLVD 480
            S  + E  LQ       D KKL  E   +E++ K    K+D L         + H  +  
Sbjct: 940  SIAEQEKCLQKISDMKLDRKKLENEVTRMEMEHKNCSVKVDKLV--------EKHTWITS 999

Query: 481  PEKLQATLAEDI-VECRDLKRALEMVTLL--DAQLKEMNPNLDSITEYRRKVEVYNERVE 540
             ++L      D   E RD  +A E +  L  D    E   N      + +  + YN  + 
Sbjct: 1000 EKRLFGNGGTDYDFESRDPHKAREELERLQTDQSSLEKRVNKKVTAMFEKAEDEYNALMT 1059

Query: 541  DLNTVTQQRDIVKKQYDEL--KKKRQMKFSSLFKKCTRFSSKLILSFYLSLTFTFMFECS 600
              N +   +  +KK  +EL  KKK  +K                                
Sbjct: 1060 KKNIIETDKSKIKKVIEELDEKKKETLK-------------------------------- 1119

Query: 601  TTWIVL--EYFEYMITL--GGDAELELVDS---LDPFSEGVVFSVRPPKKSWK-NIANLS 660
             TW+ +  ++     TL  G  ++LE  +    LD     V F        WK +++ LS
Sbjct: 1120 VTWVKVNQDFGSIFSTLLPGTMSKLEPPEGGTFLDGLEVRVAFG-----DVWKQSLSELS 1151

Query: 661  GGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFIIISLR 693
            GG+++L +L+L+ AL  +KP P+Y++DE+DAALD  +   +G  +K     +QFI++SL+
Sbjct: 1180 GGQRSLLALSLILALLLFKPAPIYILDEVDAALDLSHTQNIGRMIKSHFPHSQFIVVSLK 1151

BLAST of Cucsa.011780 vs. TAIR10
Match: AT2G27170.1 (AT2G27170.1 Structural maintenance of chromosomes (SMC) family protein)

HSP 1 Score: 83.6 bits (205), Expect = 5.6e-16
Identity = 116/514 (22.57%), Postives = 220/514 (42.80%), Query Frame = 1

Query: 28   TACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKA-KVSTPEGVPR 87
            TA   L  +VVE    +   +  L     G  TF+        L+++KA +V+ P+    
Sbjct: 545  TAGNSLFNVVVENDDISTKIIRHLNSLKGGRVTFL-------PLNRIKAPRVNYPKDSDA 604

Query: 88   LFDLIKVQ-DDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGALLEKSGT 147
            +  L K++ D + + A     G TVV +DL  ATR+A   + D    +T++G  + + G 
Sbjct: 605  IPLLKKLKFDSKFEPALGQVFGRTVVCRDLNVATRVAKNDDLD---CITMEGDQVSRKGG 664

Query: 148  MSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQLYQ---- 207
            M+GG    R  K+   +     + ++  + EK+L D+   L  I  +I   V   Q    
Sbjct: 665  MTGGFYDHRRSKL-RFMNIIMQNTKSINEKEKELEDVRRQLQVIDQQITQLVTEQQRLEA 724

Query: 208  ---VSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSL-----EAASKPKDDELNRLE 267
               + +  VEQL+  +A + +   ++     Y EK L  +     +  S     E     
Sbjct: 725  DWTLCKLQVEQLKQEIANANKQKHAIHKAIEYKEKLLGDIRTRIDQVRSSMSMKEAEMGT 784

Query: 268  ELRNFILEEEKE-ISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKV---TKIQSDI 327
            EL + +  EE+E +S+L    K L EK    Q+       +R++ +  K      I +++
Sbjct: 785  ELVDHLTPEEREQLSKLNPEIKDLKEKKFAYQA-------DRIERETRKAELEANIATNL 844

Query: 328  NKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQ 387
             +  T++      I+ D       TK        ++ L++ K ++    K+++    ++ 
Sbjct: 845  KRRITELQATIASIDDDSLPSSAGTK--------EQELDDAKLSVNEAAKELKSVCDSID 904

Query: 388  ENYKETEKLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEK 447
            E  K+ +K+           K    K+K   D+ KG+  D + KL++L  L   L  K+ 
Sbjct: 905  EKTKQIKKI-----------KDEKAKLKTLEDDCKGTLQDLDKKLEELFSLRNTLLAKQD 964

Query: 448  GYRTKLDDLQTALSKHMDQI-HKDLVDPEKLQATLAEDIVECRDL-KRALEMVTLLDAQL 507
             Y  K+  L    S   D    K++ + +K+    +E + +   + K+AL+       Q 
Sbjct: 965  EYTKKIRGLGPLSSDAFDTYKRKNIKELQKMLHRCSEQLQQFSHVNKKALDQYVNFTEQR 1014

Query: 508  KEMNPNLDSITEYRRKVEVYNERVEDLNTVTQQR 522
            +E+          + +++  +E++++L TV  QR
Sbjct: 1025 EELQ-------NRQAELDAGDEKIKELITVLDQR 1014

HSP 2 Score: 66.2 bits (160), Expect = 9.2e-11
Identity = 33/81 (40.74%), Postives = 51/81 (62.96%), Query Frame = 1

Query: 621  LSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKD--AQFII 680
            LSGG+KT+ +LAL+FA+    P P Y+ DEIDAALD +  + VG+ ++    D   QFI 
Sbjct: 1099 LSGGQKTVVALALIFAIQRCDPAPFYLFDEIDAALDPQYRTAVGNLIRRLADDYGTQFIT 1158

Query: 681  ISLRNNMFELADRLVGIYKTN 700
             + R  +  +AD++ G++  N
Sbjct: 1159 TTFRPELVRVADKIYGVFHKN 1179

BLAST of Cucsa.011780 vs. NCBI nr
Match: gi|700196240|gb|KGN51417.1| (hypothetical protein Csa_5G533470 [Cucumis sativus])

HSP 1 Score: 1250.3 bits (3234), Expect = 0.0e+00
Identity = 661/714 (92.58%), Postives = 670/714 (93.84%), Query Frame = 1

Query: 6   QELTTDIVTQNSNFAKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILE 65
           + + + + +  S  AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILE
Sbjct: 20  ENICSFVASTVSTEAKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILE 79

Query: 66  KQVDHLSKLKAKVSTPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGG 125
           KQVDHLSKLKAKVSTPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGG
Sbjct: 80  KQVDHLSKLKAKVSTPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGG 139

Query: 126 NRDFRRVVTLDGALLEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDA 185
           NRDFRRVVTLDGALLEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDA
Sbjct: 140 NRDFRRVVTLDGALLEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDA 199

Query: 186 LNRIRLRIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPK 245
           LNRIRLRIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPK
Sbjct: 200 LNRIRLRIADAVQLYQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPK 259

Query: 246 DDELNRLEELRNFILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKI 305
           DDELNRLEELRNFILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKI
Sbjct: 260 DDELNRLEELRNFILEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKI 319

Query: 306 QSDINKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKA 365
           QSDINKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKA
Sbjct: 320 QSDINKTRTDINRYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKA 379

Query: 366 FAVQENYKETEKLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELE 425
           FAVQENYKETEKLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELE
Sbjct: 380 FAVQENYKETEKLIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELE 439

Query: 426 LKEKGYRTKLDDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDA 485
           LKEKGYRTKLDDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDA
Sbjct: 440 LKEKGYRTKLDDLQTALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDA 499

Query: 486 QLKEMNPNLDSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKK 545
           QLKEMNPNLDSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKR  +F S F  
Sbjct: 500 QLKEMNPNLDSITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRLDEFMSGFNT 559

Query: 546 CTRFSSKLILSFYLSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVV 605
                        +SL    M++             MITLGGDAELELVDSLDPFSEGVV
Sbjct: 560 -------------ISLKLKEMYQ-------------MITLGGDAELELVDSLDPFSEGVV 619

Query: 606 FSVRPPKKSWKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGH 665
           FSVRPPKKSWKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGH
Sbjct: 620 FSVRPPKKSWKNIANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGH 679

Query: 666 YVKDRTKDAQFIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
           YVKDRTKDAQFIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA
Sbjct: 680 YVKDRTKDAQFIIISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 707

BLAST of Cucsa.011780 vs. NCBI nr
Match: gi|778703815|ref|XP_011655432.1| (PREDICTED: structural maintenance of chromosomes protein 4 [Cucumis sativus])

HSP 1 Score: 1249.6 bits (3232), Expect = 0.0e+00
Identity = 660/700 (94.29%), Postives = 664/700 (94.86%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS
Sbjct: 572  AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 631

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL
Sbjct: 632  TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 691

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
            LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL
Sbjct: 692  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 751

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI
Sbjct: 752  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 811

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
            LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY
Sbjct: 812  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 871

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
            KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI
Sbjct: 872  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 931

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
            HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ
Sbjct: 932  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 991

Query: 440  TALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDAQLKEMNPNLDSITE 499
            TALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDAQLKEMNPNLDSITE
Sbjct: 992  TALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDAQLKEMNPNLDSITE 1051

Query: 500  YRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFYL 559
            YRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKR  +F S F               +
Sbjct: 1052 YRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRLDEFMSGFNT-------------I 1111

Query: 560  SLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNIA 619
            SL    M++             MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNIA
Sbjct: 1112 SLKLKEMYQ-------------MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNIA 1171

Query: 620  NLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFIII 679
            NLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFIII
Sbjct: 1172 NLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFIII 1231

Query: 680  SLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
            SLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA
Sbjct: 1232 SLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 1245

BLAST of Cucsa.011780 vs. NCBI nr
Match: gi|778703815|ref|XP_011655432.1| (PREDICTED: structural maintenance of chromosomes protein 4 [Cucumis sativus])

HSP 1 Score: 49.7 bits (117), Expect = 2.5e-02
Identity = 77/352 (21.88%), Postives = 145/352 (41.19%), Query Frame = 1

Query: 204 EKAVEQLEMLLA--KSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFILE 263
           E  +E LE ++   K  + ID    Q   L ++ + +    K  + E + LE ++N   E
Sbjct: 193 EGFLEYLEDIIGTIKYVEMIDESNKQLEALNEKRTGVVQMVKLAEKERDGLEGVKN---E 252

Query: 264 EE----KEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDIN 323
            E    KE+S L     K  EKA ++  +              ++T++Q +++    +  
Sbjct: 253 AEAYMLKELSHL-----KWREKASKLAHEDTT----------KRITELQDEVSTLEANKK 312

Query: 324 RYKVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEK 383
             + +I      +K+L    E + K KE L+ +    + KFKD E      +++ K  E 
Sbjct: 313 TEREKIRETSKELKELEAVHEKNMKRKEELDNDLRRSKEKFKDFE------RQDIKYRED 372

Query: 384 LIHLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDD 443
           L H+++           K+KK  D+L+        K+  L+K  +E              
Sbjct: 373 LKHIKQ-----------KIKKLDDKLEKDST----KIDGLRKECEE-----------STS 432

Query: 444 LQTALSKHMDQIHKDLVDPEKLQATLAEDI-VECRDLKRALEMVTLLDAQLKEMNPNLDS 503
           L   L + + Q  K L D EK+   + E   VE    +  L +V +      E+ P    
Sbjct: 433 LIPKLEESIPQFQKLLTDEEKILDEIQESSKVETERYRSELAIVRV------ELEPWEKQ 485

Query: 504 ITEYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTR 549
           +TE+R K+   N    +   ++Q+ +  +   D+ +K+      ++ +K T+
Sbjct: 493 LTEHRGKL---NVACTESKLLSQKHEGGRAALDDARKQMVNILKNIEEKSTK 485

HSP 2 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 643/700 (91.86%), Postives = 658/700 (94.00%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS
Sbjct: 572  AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 631

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL
Sbjct: 632  TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 691

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
            LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAF+KAEKDLSDMVDALN+IR+RIADAVQL
Sbjct: 692  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFIKAEKDLSDMVDALNKIRIRIADAVQL 751

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            YQVSEKAVEQLEMLLAK QQDIDSLTSQHSYLEKQLSSLEAASKPKDDEL RLEELRNFI
Sbjct: 752  YQVSEKAVEQLEMLLAKCQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELKRLEELRNFI 811

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
             EEEKEISRLVLGSKKLTEKALE+QSQIENAGGERLKAQKSKVTKIQSDI+KTRTDINRY
Sbjct: 812  SEEEKEISRLVLGSKKLTEKALELQSQIENAGGERLKAQKSKVTKIQSDISKTRTDINRY 871

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
            KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI
Sbjct: 872  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 931

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
            HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ
Sbjct: 932  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 991

Query: 440  TALSKHMDQIHKDLVDPEKLQATLAEDIVECRDLKRALEMVTLLDAQLKEMNPNLDSITE 499
            TAL+KHM+QI+KDLVDP+KLQATLAEDIVEC DLKRALEMV LLDAQLKEMNPNLDSITE
Sbjct: 992  TALAKHMEQINKDLVDPKKLQATLAEDIVECCDLKRALEMVMLLDAQLKEMNPNLDSITE 1051

Query: 500  YRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFYL 559
            YRRKVEVY+ERVEDLNTVTQQRD +KKQYDELKKKR  +F S F               +
Sbjct: 1052 YRRKVEVYDERVEDLNTVTQQRDAMKKQYDELKKKRLDEFMSGFNT-------------I 1111

Query: 560  SLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNIA 619
            SL    M++             MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNIA
Sbjct: 1112 SLKLKEMYQ-------------MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNIA 1171

Query: 620  NLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFIII 679
            NLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFIII
Sbjct: 1172 NLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFIII 1231

Query: 680  SLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
            SLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA
Sbjct: 1232 SLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 1245

BLAST of Cucsa.011780 vs. NCBI nr
Match: gi|224140989|ref|XP_002323859.1| (hypothetical protein POPTR_0017s11950g [Populus trichocarpa])

HSP 1 Score: 988.4 bits (2554), Expect = 6.5e-285
Identity = 520/701 (74.18%), Postives = 587/701 (83.74%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTACPGLDYIVVET+ AAQACVELLRRE LGVATFMILEKQVDH SK+K  VS
Sbjct: 576  AKYDVAISTACPGLDYIVVETTAAAQACVELLRREKLGVATFMILEKQVDHSSKMKHNVS 635

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPEGVPRLFDL++VQD+RMKLAF+AALGNTVVAKDL+QATRIAYGGN +FRRVVTLDGAL
Sbjct: 636  TPEGVPRLFDLVRVQDERMKLAFYAALGNTVVAKDLDQATRIAYGGNLEFRRVVTLDGAL 695

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
             EKSGTMSGGG  PRGGKMGTSIR+ SVS EA   AEK+LS MVD LN IR RIAD+V+ 
Sbjct: 696  FEKSGTMSGGGTKPRGGKMGTSIRATSVSGEAVTSAEKELSTMVDRLNGIRQRIADSVKH 755

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            YQ SEKA+  LEM LAKSQ++IDSL ++HSYLEKQL SL+AAS+PK DEL+RLEEL+  I
Sbjct: 756  YQASEKAIAHLEMELAKSQKEIDSLNTEHSYLEKQLGSLKAASEPKKDELDRLEELKRII 815

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
            + EEKEI RL+ GSKKL EKALE+QS+IENAGGERLK+QK+KV +IQSD++K  T+INR+
Sbjct: 816  VTEEKEIDRLIQGSKKLKEKALELQSKIENAGGERLKSQKAKVNRIQSDMDKNSTEINRH 875

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
            KVQIE+    +KKLTK IEDS+KEKERL EE+  L+G FK+IE KAFAVQENYK+T++LI
Sbjct: 876  KVQIETGHKMIKKLTKGIEDSRKEKERLTEEREKLRGIFKEIEEKAFAVQENYKKTQELI 935

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
               +EV D +K+ Y KVKK +DEL+ SEVD +Y+LQD+KK YKELELK KGY+ KLDDLQ
Sbjct: 936  DQHKEVLDKAKSEYEKVKKIVDELRASEVDADYRLQDMKKSYKELELKGKGYKKKLDDLQ 995

Query: 440  TALSKHMDQIHKDLVDPEKLQATLAE-DIVECRDLKRALEMVTLLDAQLKEMNPNLDSIT 499
             AL  HM+Q  K+L DPEKLQATLA+  + E  DLKRALE V LL+AQLK+MNPNLDSI+
Sbjct: 996  NALLHHMEQTQKELEDPEKLQATLADKTLAEACDLKRALERVVLLEAQLKDMNPNLDSIS 1055

Query: 500  EYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFY 559
            EYRRKV  YNERVE+LN VTQQRD +K+QYDE +KKR                 L+ SF 
Sbjct: 1056 EYRRKVSSYNERVEELNLVTQQRDDIKRQYDEWRKKR-----------------LVFSFL 1115

Query: 560  LSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 619
            L     FM   +T  + L+    MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI
Sbjct: 1116 LD---EFMAGFNTISLKLKEMYQMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 1175

Query: 620  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 679
            ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII
Sbjct: 1176 ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 1235

Query: 680  ISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
            ISLRNNMFELADRLVGIYKT+NCTKSITINP SF VC+  A
Sbjct: 1236 ISLRNNMFELADRLVGIYKTDNCTKSITINPGSFVVCQNAA 1256

BLAST of Cucsa.011780 vs. NCBI nr
Match: gi|743878847|ref|XP_011035754.1| (PREDICTED: structural maintenance of chromosomes protein 4 [Populus euphratica])

HSP 1 Score: 983.4 bits (2541), Expect = 2.1e-283
Identity = 516/701 (73.61%), Postives = 587/701 (83.74%), Query Frame = 1

Query: 20   AKYDVAISTACPGLDYIVVETSGAAQACVELLRRENLGVATFMILEKQVDHLSKLKAKVS 79
            AKYDVAISTACPGLDYIVVET+ AAQACVELLRRE LGVATFMILEKQVDH SK+K  VS
Sbjct: 576  AKYDVAISTACPGLDYIVVETTAAAQACVELLRREKLGVATFMILEKQVDHSSKMKHNVS 635

Query: 80   TPEGVPRLFDLIKVQDDRMKLAFFAALGNTVVAKDLEQATRIAYGGNRDFRRVVTLDGAL 139
            TPEGVPRLFDL++VQD+RMKLAF+AALGNTVVAKDL+QATRIAYGGN +FRRVVTLDGAL
Sbjct: 636  TPEGVPRLFDLVRVQDERMKLAFYAALGNTVVAKDLDQATRIAYGGNLEFRRVVTLDGAL 695

Query: 140  LEKSGTMSGGGRMPRGGKMGTSIRSASVSKEAFVKAEKDLSDMVDALNRIRLRIADAVQL 199
             EKSGTMSGGG  PRGGKMGTSIR+ SVS EA   AEK+LS MVD LN IR RIAD+V+ 
Sbjct: 696  FEKSGTMSGGGTKPRGGKMGTSIRATSVSGEAVTSAEKELSTMVDRLNGIRQRIADSVKH 755

Query: 200  YQVSEKAVEQLEMLLAKSQQDIDSLTSQHSYLEKQLSSLEAASKPKDDELNRLEELRNFI 259
            YQ SEKA+  LEM LAKSQ++IDSL ++HSYLEKQL SL+AAS+PK DEL+RLEEL+  I
Sbjct: 756  YQASEKAIAHLEMELAKSQKEIDSLNTEHSYLEKQLGSLKAASEPKKDELDRLEELKMII 815

Query: 260  LEEEKEISRLVLGSKKLTEKALEIQSQIENAGGERLKAQKSKVTKIQSDINKTRTDINRY 319
            + EEKEI RL+ GSKKL EKALE+QS+IENAGGERLK+QK+KV +IQSD++K  T+INR+
Sbjct: 816  MTEEKEIDRLIQGSKKLKEKALELQSKIENAGGERLKSQKAKVNRIQSDMDKNSTEINRH 875

Query: 320  KVQIESDQATMKKLTKAIEDSKKEKERLEEEKNNLQGKFKDIEVKAFAVQENYKETEKLI 379
            KVQIE+    +KKLTK IEDS+KEKE+L EE+  L+G FK+IE KAFAVQENYK+T++LI
Sbjct: 876  KVQIETGHKMIKKLTKGIEDSRKEKEQLTEEREKLRGIFKEIEEKAFAVQENYKKTQELI 935

Query: 380  HLQEEVCDTSKANYNKVKKTMDELKGSEVDTEYKLQDLKKLYKELELKEKGYRTKLDDLQ 439
               +EV D +K+ Y KVKK +DEL+ SEVD +Y+LQD+KK YKELELK KGY+ KLDDLQ
Sbjct: 936  DQHKEVLDKAKSEYEKVKKIVDELRASEVDADYRLQDMKKSYKELELKGKGYKKKLDDLQ 995

Query: 440  TALSKHMDQIHKDLVDPEKLQATLAE-DIVECRDLKRALEMVTLLDAQLKEMNPNLDSIT 499
             AL +HM+Q  K+L DPEKLQATLA+  + E  DLKRALE V LL+AQLK+MNPNLDSI+
Sbjct: 996  NALLRHMEQTQKELEDPEKLQATLADKTLAEACDLKRALERVVLLEAQLKDMNPNLDSIS 1055

Query: 500  EYRRKVEVYNERVEDLNTVTQQRDIVKKQYDELKKKRQMKFSSLFKKCTRFSSKLILSFY 559
            EYRRKV +YNERVE+LN VTQQRD +K+QYDE +KKR  +F + F               
Sbjct: 1056 EYRRKVSLYNERVEELNLVTQQRDDIKRQYDEWRKKRLDEFMAGFNT------------- 1115

Query: 560  LSLTFTFMFECSTTWIVLEYFEYMITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 619
            +SL    M++             MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI
Sbjct: 1116 ISLKLKEMYQ-------------MITLGGDAELELVDSLDPFSEGVVFSVRPPKKSWKNI 1175

Query: 620  ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 679
            ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII
Sbjct: 1176 ANLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFKNVSIVGHYVKDRTKDAQFII 1235

Query: 680  ISLRNNMFELADRLVGIYKTNNCTKSITINPRSFSVCEKIA 720
            ISLRNNMFELADRLVGIYKT+NCTKSITINP SF VC+  A
Sbjct: 1236 ISLRNNMFELADRLVGIYKTDNCTKSITINPGSFVVCQNAA 1250