Cucsa.010570 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.010570 vs. Swiss-Prot
Match: SYMM_ARATH (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=OVA1 PE=2 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 3.3e-269
Identity = 455/608 (74.84%), Postives = 521/608 (85.69%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTR-LHFCQNLLSFPSHSSSKRAIFCTSTS 60
           MAARINTS+  +L  L PF++ LN KP  FR     F + L  +   SS KRA++CTS+S
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTKPFSFRRNSFRFSKKLPYYSQFSSGKRALYCTSSS 60

Query: 61  NFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDE 120
             ++ D G   + FV+TTPLYYVNA PHMGSAYTTIAAD++ARFQRLLGKKVIFITGTDE
Sbjct: 61  QESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFITGTDE 120

Query: 121 HGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKV 180
           HGEKIAT+AA  G  P EHCD+IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKEFY++V
Sbjct: 121 HGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEFYARV 180

Query: 181 LANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLL 240
            ANGDIYRADYEGLYCVNCEEYKDEKELL +NCCP+H  PC +RKEDNYFFALSKYQ+ L
Sbjct: 181 FANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKYQKPL 240

Query: 241 EETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALL 300
           E+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D+KQTIYVWFDALL
Sbjct: 241 EDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWFDALL 300

Query: 301 GYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGH 360
           GYISAL+E+ +Q NL+  V+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKMVFGH
Sbjct: 301 GYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKMVFGH 360

Query: 361 GFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAH 420
           GFLTKDGMKMGKSLGNTLEP ELV+KFG DAVRYFFLREVEFG+DGDY+EDRFI IVNAH
Sbjct: 361 GFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKIVNAH 420

Query: 421 LANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSA 480
           LANTIGNLLNRTLGLLKKNC+STLVVDST AA+G   KD VE+LV KAR NYE L+LSSA
Sbjct: 421 LANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSLSSA 480

Query: 481 CEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLS 540
           CEAVLEIGNAGN+YMD+RAPW LFKQGG S+  AAKDLV+ILE MR+IA+ALSPV P LS
Sbjct: 481 CEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVAPCLS 540

Query: 541 WRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVK 600
            RIY+QLGY++DQF++ TW++TKWGGLK GQVM   +PVFARIE   +N E+E  +K  K
Sbjct: 541 LRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIE---LNPEKEEDEKKPK 600

Query: 601 SKKKIAQA 608
             KK  +A
Sbjct: 601 VGKKTGKA 602

BLAST of Cucsa.010570 vs. Swiss-Prot
Match: SYM_SYNY3 (Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=metG PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 7.3e-160
Identity = 282/530 (53.21%), Postives = 362/530 (68.30%), Query Frame = 1

Query: 65  DSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEHGEKI 124
           DS   T  F +TTPLYYVN  PH+GSAYTT+ AD +ARF+RL G  V+ ITGTDEHG+KI
Sbjct: 3   DSSVPT--FSVTTPLYYVNDVPHLGSAYTTVVADTLARFKRLQGYDVLMITGTDEHGQKI 62

Query: 125 ATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGD 184
              A  Q   P  HCD     +K LW+ L++ YD+F RTTDP+H AIVK+F+ +V   GD
Sbjct: 63  QRTAEAQELDPQTHCDRTVVKFKELWRSLNILYDRFSRTTDPRHLAIVKDFFQRVWDKGD 122

Query: 185 IYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCAS-RKEDNYFFALSKYQRLLEETL 244
           IY A  +G YCV CEE+K++++LL DN CP+H    A  R E+NYFF LS+YQ  LEE  
Sbjct: 123 IYLAQQQGWYCVACEEFKEKRDLLEDNHCPLHPNRKAEWRDEENYFFRLSRYQHPLEELY 182

Query: 245 TQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLGYIS 304
            Q P F+QP+ R NEV +++  GL+DFSISR +++WG P+PND   TIYVWFDALLGY++
Sbjct: 183 AQRPEFIQPSSRRNEVLNFVAQGLQDFSISRVNLDWGFPLPNDPNHTIYVWFDALLGYVT 242

Query: 305 ALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLT 364
           AL +  E+PNL   +   WP +LHLIGKDILRFHAVYWPAMLMSA L +P  VFGHGFLT
Sbjct: 243 ALLDEDEEPNLTNALVKWWPINLHLIGKDILRFHAVYWPAMLMSAELAIPAQVFGHGFLT 302

Query: 365 KDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANT 424
           KDG KMGKSLGNT++P +L+ ++G DA RY+FL+E+EFG DGD+ E RF+N++NA LAN 
Sbjct: 303 KDGQKMGKSLGNTVDPLDLINRYGEDAFRYYFLKEIEFGKDGDFNEQRFVNVLNADLAND 362

Query: 425 IGNLLNRTLGLLKKNCQST-LVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSACEA 484
           +GNLLNRTLG++KK CQ     V +T  A  N  K     L  +  + YE L+ + ACEA
Sbjct: 363 LGNLLNRTLGMVKKYCQGQGPQVMATDLAPDNPLKALGSHLGEEVSSAYERLSFTDACEA 422

Query: 485 VLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSWRI 544
           +  +  AGN Y+D+ APW LFKQG  S       L  +LE++R+ A  LSP+ PRLS +I
Sbjct: 423 IFTLVRAGNKYIDDMAPWKLFKQG--SQKEVEDVLYSVLESIRLSAYLLSPIVPRLSTKI 482

Query: 545 YAQLGYSKDQFD--------AATWN-ETKWGGLKAGQVMAPPNPVFARIE 584
           Y QLG++ D FD        A  +N    WG L A Q + P  P+F ++E
Sbjct: 483 YQQLGFTWD-FDQWRSPLEQAEEFNRHQSWGQLGANQNLPPAQPIFTKLE 527

BLAST of Cucsa.010570 vs. Swiss-Prot
Match: SYM_NOSS1 (Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / UTEX 2576) GN=metG PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 9.8e-157
Identity = 277/522 (53.07%), Postives = 362/522 (69.35%), Query Frame = 1

Query: 69  ATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEHGEKIATAA 128
           A   F +TTPLYYVN  PH+GSAYTT+AADAVARFQ+LLG+ V+ ITGTDEHG+KI  +A
Sbjct: 7   AEKTFALTTPLYYVNDVPHIGSAYTTMAADAVARFQKLLGRDVLLITGTDEHGQKIQRSA 66

Query: 129 ATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRA 188
            + G  P E CD I  ++ +LW+ L++ YD+F RTT  +H+AIV EF+++V   GDIY+ 
Sbjct: 67  ESLGKAPQEFCDEIVPSFMSLWRLLNIQYDRFSRTTAVRHKAIVDEFFARVWEAGDIYQG 126

Query: 189 DYEGLYCVNCEEYKDEKELLADNCCPIHL-KPCASRKEDNYFFALSKYQRLLEETLTQNP 248
             +G YCV+CEE+K+E+ELL  N CPIH+ K    R E NYFF LSKYQ  LEE    +P
Sbjct: 127 QQKGWYCVSCEEFKEERELLEGNRCPIHVNKEVEWRDEQNYFFRLSKYQTQLEEFYQSHP 186

Query: 249 GFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLGYISALSE 308
            F+QP  R NEV +++  GL+DFSISR +++WG PVPND K T+YVWFDALL Y++AL +
Sbjct: 187 DFIQPESRRNEVLNFVSQGLQDFSISRVNLDWGFPVPNDPKHTLYVWFDALLAYVTALLD 246

Query: 309 NGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGM 368
             ++P L+  +   WP +LHLIGKDILRFHAVYWPAML+SAGL LP  VFGHGFLTKDG 
Sbjct: 247 PEDEPTLENALGKWWPINLHLIGKDILRFHAVYWPAMLLSAGLPLPDRVFGHGFLTKDGQ 306

Query: 369 KMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNL 428
           KMGKSLGNT++P  LV+++GSDAVRY+FL+E+EFG DGD+ E RFI+++NA LAN +GNL
Sbjct: 307 KMGKSLGNTVDPVGLVQQYGSDAVRYYFLKEIEFGKDGDFNEVRFIHVLNADLANDLGNL 366

Query: 429 LNRTLGLLKKNCQS-TLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSACEAVLEI 488
           LNRTL ++KK C +  L + +      N  K    +L  K +  YE LA + AC  VL +
Sbjct: 367 LNRTLNMVKKYCANYALSITNEDIPAENTLKALGVDLGAKVKQAYEVLAFNQACGVVLSL 426

Query: 489 GNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYAQL 548
             A N ++D++APWSL+KQ           L  +LE++R+ A  LSPV P +S  IY QL
Sbjct: 427 VQASNKFIDDQAPWSLYKQ--ERQQEVETVLYTVLESVRLAAYLLSPVIPNISSNIYQQL 486

Query: 549 GYS---KDQFDAATWN-ETKWGGLKAGQVMAPPNPVFARIEQ 585
           G+     +Q D   +    +WG L   Q +  P P+F RIEQ
Sbjct: 487 GFGINFNEQTDITPFAIHAQWGLLSNKQQLGQPQPIFKRIEQ 526

BLAST of Cucsa.010570 vs. Swiss-Prot
Match: SYM_THEEB (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) GN=metG PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.1e-151
Identity = 266/517 (51.45%), Postives = 348/517 (67.31%), Query Frame = 1

Query: 73  FVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEHGEKIATAAATQG 132
           F +TTPLYYVNA PH+GSAYTTIAAD +ARF RL G +V FITGTDEHG+KI   A  +G
Sbjct: 5   FSLTTPLYYVNALPHIGSAYTTIAADVLARFYRLQGYQVRFITGTDEHGQKIERTAQQRG 64

Query: 133 STPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 192
            +P  HCD I+  ++ LW+ L++ YD+F RTT P+H AIV EF+ +V  NGDIY    +G
Sbjct: 65  LSPQAHCDEIAAGFQALWQQLNIHYDRFSRTTSPRHHAIVNEFFQRVWDNGDIYLGQQQG 124

Query: 193 LYCVNCEEYKDEKELLADNCCPIHLKPCAS-RKEDNYFFALSKYQRLLEETLTQNPGFVQ 252
            YCV CEE+K+E+ELL    CPIH+      R E NYFF LSKYQ+ L +   ++P FVQ
Sbjct: 125 WYCVECEEFKEERELLEGRRCPIHVNRTVEWRDERNYFFRLSKYQQALLDHYAEHPDFVQ 184

Query: 253 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLGYISALSENGEQ 312
           P  R NEV S+I  GL+DFSISR ++ WG PVP D +QT+YVWFDALLGY++AL E  ++
Sbjct: 185 PPSRRNEVLSFIERGLQDFSISRVNLAWGFPVPTDPEQTLYVWFDALLGYVTALLEPEDE 244

Query: 313 PNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 372
           P L   + + WP +LH+IGKDILRFH + WPAMLMSAGL LP+ +F HGFLTKDG KMGK
Sbjct: 245 PTLANALKTWWPINLHIIGKDILRFHGISWPAMLMSAGLPLPEQIFVHGFLTKDGQKMGK 304

Query: 373 SLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 432
           SLGNTL+P  LV ++G+DAVRY+F++EVEFG DGD++E RF+ I+NA LAN +GNLLNRT
Sbjct: 305 SLGNTLDPFALVAQYGADAVRYYFMKEVEFGRDGDFSETRFVTILNADLANDLGNLLNRT 364

Query: 433 LGLLKKNCQSTLV-VDSTAAADGNAFKDKVEELVVKARNNYEELALSSACEAVLEIGNAG 492
           L +  K     +  V   A    +  +   E L       Y +LA    C+  L +  AG
Sbjct: 365 LKMAWKYTDGKVPNVQGAAIPREHPLRQLAEHLSQTYGQGYRQLAFHEVCQQALTLARAG 424

Query: 493 NSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYAQLGYS- 552
           N ++DE APW  ++ G   + A A+ L  +LE++R++A  L+P+ P+LS  IY QLGYS 
Sbjct: 425 NKFLDEEAPWKRYRAG--ETAAVAEILYCVLESVRLVAYVLAPIIPQLSEAIYGQLGYSI 484

Query: 553 ----KDQFDAATWNETKWGGLKAGQVMAPPNPVFARI 583
                   D     + +WG L A Q +A P P+F ++
Sbjct: 485 RFNGSIAPDLLGDRQAQWGVLPASQPLANPEPIFQKL 519

BLAST of Cucsa.010570 vs. Swiss-Prot
Match: SYM_AQUAE (Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) GN=metG PE=1 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.8e-118
Identity = 219/477 (45.91%), Postives = 292/477 (61.22%), Query Frame = 1

Query: 73  FVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEHGEKIATAAATQG 132
           F +TTP+YYVN  PH+G AYTTIAAD +AR+ RL    V F+TGTDEHG KI   A   G
Sbjct: 7   FYVTTPIYYVNDVPHLGHAYTTIAADTIARYYRLRDYDVFFLTGTDEHGLKIQKKAEELG 66

Query: 133 STPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 192
            +P E  D  +E +K LW+ L + Y KFIRTTDP H   V++ + +    GDIY  +YEG
Sbjct: 67  ISPKELVDRNAERFKKLWEFLKIEYTKFIRTTDPYHVKFVQKVFEECYKRGDIYLGEYEG 126

Query: 193 LYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLEETLTQNPGFVQP 252
            YCV CEE+K E EL  D+ CPIH K C   KE +YFF LSKYQ  L E   +NP F+QP
Sbjct: 127 WYCVGCEEFKSEAELAEDHTCPIHQKKCEYIKEPSYFFRLSKYQDKLLELYEKNPEFIQP 186

Query: 253 AFRLNEVQSWIRSGLKDFSIS--RASVEWGIPVPNDEKQTIYVWFDALLGYISALSENGE 312
            +R NE+ S+++ GLKD S++  R+ V+WGIPVP D + TIYVWFDAL  YISA      
Sbjct: 187 DYRRNEIISFVKQGLKDLSVTRPRSRVKWGIPVPFDPEHTIYVWFDALFNYISA------ 246

Query: 313 QPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMG 372
              L++ V   WPA LHL+GKDILRFH VYWPA LMS G +LPK VF HG+ T +G KM 
Sbjct: 247 ---LEDKVEIYWPADLHLVGKDILRFHTVYWPAFLMSLGYELPKKVFAHGWWTVEGKKMS 306

Query: 373 KSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNR 432
           K+LGN ++P E+V+++G D VRYF LREV FG DGD+++   +N +N  LAN IGNL +R
Sbjct: 307 KTLGNVVDPYEVVQEYGLDEVRYFLLREVPFGQDGDFSKKAILNRINGELANEIGNLYSR 366

Query: 433 TLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSACEAVLEIGNAG 492
            + +  K      +    + A    +    +E +    N  E++    A E +L+  +  
Sbjct: 367 VVNMAHK-----FLGGEVSGARDEEYAKIAQESIKNYENYMEKVNFYKAIEEILKFTSYL 426

Query: 493 NSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYAQLG 548
           N Y+DE+ PW+L K+         K L  +++ + ++   L P+TP         LG
Sbjct: 427 NKYVDEKQPWALNKE--RKKEELQKVLYALVDGLFVLTHLLYPITPNKMKEALQMLG 467

BLAST of Cucsa.010570 vs. TrEMBL
Match: A0A0A0KQV7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G512910 PE=3 SV=1)

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 611/611 (100.00%), Postives = 611/611 (100.00%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 60
           MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 60

Query: 61  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 120
           FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH
Sbjct: 61  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 120

Query: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180
           GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL
Sbjct: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180

Query: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240
           ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE
Sbjct: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240

Query: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 300
           ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG
Sbjct: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 300

Query: 301 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360
           YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG
Sbjct: 301 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360

Query: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420
           FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL
Sbjct: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420

Query: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 480
           ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC
Sbjct: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 480

Query: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540
           EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW
Sbjct: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540

Query: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS 600
           RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS
Sbjct: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS 600

Query: 601 KKKIAQAVAES 612
           KKKIAQAVAES
Sbjct: 601 KKKIAQAVAES 611

BLAST of Cucsa.010570 vs. TrEMBL
Match: D7TLW0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g00810 PE=3 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 1.4e-279
Identity = 484/614 (78.83%), Postives = 543/614 (88.44%), Query Frame = 1

Query: 2   AARINTSIQTSLWLLN-PFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 61
           A R+++SIQ+SL +L+ P +S+      H +TRLHF + L S PS SS  RA+FC+  ++
Sbjct: 3   AVRVHSSIQSSLCILSHPLYSTSR----HLKTRLHFPRPLFSSPSFSSG-RALFCSCRAD 62

Query: 62  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 121
                    +D FV+TTPLYYVNA PHMGSAYTTIAADA+ARFQRL GKKVIFITGTDEH
Sbjct: 63  NFKGSEREVSDSFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLSGKKVIFITGTDEH 122

Query: 122 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 181
           GEKIATAAA  GS+PSEHCD+IS+AYKTLW+DLD+AYDKFIRTT+PKHEAIVKEFYS+VL
Sbjct: 123 GEKIATAAAACGSSPSEHCDVISQAYKTLWQDLDIAYDKFIRTTNPKHEAIVKEFYSRVL 182

Query: 182 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 241
           ANGDIYRADYEGLYC+NCEEYKDEKELL +NCCP+HLKPC  RKEDNYFFALSKYQ+LLE
Sbjct: 183 ANGDIYRADYEGLYCINCEEYKDEKELLENNCCPMHLKPCVLRKEDNYFFALSKYQKLLE 242

Query: 242 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 301
           ETLTQNP FVQP+FRLNEVQSWI+SGL+DFSISRASV+WGIPVPND KQTIYVWFDALLG
Sbjct: 243 ETLTQNPDFVQPSFRLNEVQSWIKSGLRDFSISRASVDWGIPVPNDNKQTIYVWFDALLG 302

Query: 302 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 361
           YISALSE+ EQP+LQ  V+SGWPASLHLIGKDILRFHAVYWPAML+SAGL LPKMVFGHG
Sbjct: 303 YISALSEDNEQPDLQSAVSSGWPASLHLIGKDILRFHAVYWPAMLLSAGLSLPKMVFGHG 362

Query: 362 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 421
           FLTKDGMKMGKSLGNTLEPN+LV++FGSDAVRYFFLREVEFG+DGDY+EDRFINIVNAHL
Sbjct: 363 FLTKDGMKMGKSLGNTLEPNDLVQRFGSDAVRYFFLREVEFGNDGDYSEDRFINIVNAHL 422

Query: 422 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 481
           ANTIGNLLNRTLGLLKKNCQSTL VDST AA+GNAFKD V++LV KA+  YE L+LSSAC
Sbjct: 423 ANTIGNLLNRTLGLLKKNCQSTLAVDSTLAAEGNAFKDTVDKLVEKAKFQYENLSLSSAC 482

Query: 482 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 541
           EAVLEIGNAGN Y+DERAPWSLFKQG A+S AAAKDLV+ILEAMRIIAIALSPVTPRLS 
Sbjct: 483 EAVLEIGNAGNLYIDERAPWSLFKQGAAASEAAAKDLVIILEAMRIIAIALSPVTPRLSL 542

Query: 542 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIE-QAIVNGEEEAPKKPVK 601
           RIY QLGY+K+QF+A TW+ET+WGGLK+GQVMA P PVFARIE +A V    EA KK VK
Sbjct: 543 RIYEQLGYTKEQFNATTWSETEWGGLKSGQVMAQPKPVFARIEIEAEVGDGGEAAKKVVK 602

Query: 602 SKKKI--AQAVAES 612
            K+KI   Q VA++
Sbjct: 603 KKEKIPHTQGVAQA 611

BLAST of Cucsa.010570 vs. TrEMBL
Match: B9HTK5_POPTR (Methionyl-tRNA synthetase family protein OS=Populus trichocarpa GN=POPTR_0010s21430g PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 4.1e-279
Identity = 476/607 (78.42%), Postives = 520/607 (85.67%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 60
           MAARIN S+Q +L LLNP + S+N K THF+T ++F +NL+ FP     +R  FCT   +
Sbjct: 1   MAARINYSVQNTLCLLNPLNYSINAKTTHFKTGINFSKNLI-FPR--KLRRPTFCTCAIS 60

Query: 61  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 120
            N   S  A + FV+TTPLYYVNA PHMGSAYTTIAADA+ARFQRLLGKKVIF+TGTDEH
Sbjct: 61  NNHMQSSEAAETFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLLGKKVIFVTGTDEH 120

Query: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180
           GEKIA +AA  GSTPSEHCD+IS+AYKTLWKDL++ YDKFIRTTDPKHE IVKEFYS+VL
Sbjct: 121 GEKIAASAAAAGSTPSEHCDVISQAYKTLWKDLEITYDKFIRTTDPKHEGIVKEFYSRVL 180

Query: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240
           A GDIYRADYEGLYCVNCEEYKDEKELL + CCP HLKPC  RKEDNYFFALSKYQ+ LE
Sbjct: 181 AKGDIYRADYEGLYCVNCEEYKDEKELLDNKCCPTHLKPCIERKEDNYFFALSKYQKQLE 240

Query: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 300
           E LTQNP FVQP FRLNEVQSWI+SGLKDFSISRASV+WGIPVPND KQTIYVWFDALLG
Sbjct: 241 ENLTQNPKFVQPPFRLNEVQSWIKSGLKDFSISRASVDWGIPVPNDNKQTIYVWFDALLG 300

Query: 301 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360
           YISALS   EQP+LQ  V+SGWPASLHLIGKDILRFHAVYWPAMLMSAG+ LPK VFGHG
Sbjct: 301 YISALSAENEQPDLQTAVSSGWPASLHLIGKDILRFHAVYWPAMLMSAGVDLPKKVFGHG 360

Query: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420
           FLTKDGMKMGKSLGNTLEPNELV  FGSDAVRYFFLREVEFGSDGDY+E+RFINIVNAHL
Sbjct: 361 FLTKDGMKMGKSLGNTLEPNELVHNFGSDAVRYFFLREVEFGSDGDYSEERFINIVNAHL 420

Query: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 480
           ANTIGNLLNRTLGLLKKNCQSTL VDS  AA+GNA KD +E+LV KAR +YE L+LSSAC
Sbjct: 421 ANTIGNLLNRTLGLLKKNCQSTLAVDSAVAAEGNALKDTIEKLVEKARIHYENLSLSSAC 480

Query: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540
           EAVLEIGNAGN+YMDE APW+ FKQGGA+S AAAKDLV+ILEAMRI+AIALSPV P   W
Sbjct: 481 EAVLEIGNAGNAYMDENAPWTRFKQGGAASEAAAKDLVIILEAMRIVAIALSPVAPSFCW 540

Query: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS 600
           RIY QLGYSKD F+  TW+ TKWGGLK GQVMA P PVFARIE      +E A KKPVKS
Sbjct: 541 RIYEQLGYSKDHFNTVTWSGTKWGGLKGGQVMAQPKPVFARIENKTELEDEAATKKPVKS 600

Query: 601 KKKIAQA 608
           K+K  QA
Sbjct: 601 KQKKPQA 604

BLAST of Cucsa.010570 vs. TrEMBL
Match: V4VAK1_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10030995mg PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 7.1e-279
Identity = 484/616 (78.57%), Postives = 528/616 (85.71%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 60
           MAARIN SIQ SLWLLNP HS      THFR RL F QNL S      SK A+FC+  +N
Sbjct: 1   MAARINYSIQNSLWLLNPLHSKAT---THFRNRLKFRQNLFSL-----SKGAVFCSYATN 60

Query: 61  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 120
            +   +  A DPFV+TTPLYYVNA PHMGSAYTTIAADA+ARFQRLLGKKVIFITGTDEH
Sbjct: 61  TDKSGTEPA-DPFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEH 120

Query: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180
           GEKIA AAA  GS+PSEHCD++S+AYKTLWKDLD+AYDKFIRTT+PKHEAIVKEFYS VL
Sbjct: 121 GEKIAAAAADGGSSPSEHCDVVSQAYKTLWKDLDIAYDKFIRTTEPKHEAIVKEFYSSVL 180

Query: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240
           ANGDIY+ADYEGLYCVNCEEYKDEKELL + CCPIHLKPC +RKEDNYFFALSKYQ+LLE
Sbjct: 181 ANGDIYKADYEGLYCVNCEEYKDEKELLENQCCPIHLKPCVARKEDNYFFALSKYQKLLE 240

Query: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 300
           +TLT+NP FVQP+FRLNEVQ WI+SGL+DFSISRASV+WGIPVPND KQTIYVWFDALLG
Sbjct: 241 DTLTENPDFVQPSFRLNEVQGWIKSGLRDFSISRASVDWGIPVPNDNKQTIYVWFDALLG 300

Query: 301 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360
           YISALSE+ +QP+L+  V+SGWPASLHLIGKDILRFHAVYWPAMLMSAG+ LPK VFGHG
Sbjct: 301 YISALSEDRDQPSLRTAVSSGWPASLHLIGKDILRFHAVYWPAMLMSAGIGLPKKVFGHG 360

Query: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420
           FLTKDGMKMGKSLGNTLEPNELV  FGSDAVRYFFL+EVEFG+DGDY+EDRFI IVNAHL
Sbjct: 361 FLTKDGMKMGKSLGNTLEPNELVHNFGSDAVRYFFLKEVEFGNDGDYSEDRFIKIVNAHL 420

Query: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 480
           ANTIGNLLNRTLGLLKKNCQSTLVVDS  AA+G AFKD VE+LV  AR +YE L+LSSAC
Sbjct: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSAVAAEGTAFKDTVEKLVQTARGHYENLSLSSAC 480

Query: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540
           EAVL+I NAGNSYMDE APWS FKQGGA+S AAAKDLV+ILEAMRIIAIALSPVTP L W
Sbjct: 481 EAVLDISNAGNSYMDEHAPWSRFKQGGAASEAAAKDLVIILEAMRIIAIALSPVTPNLCW 540

Query: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIE--QAIVNGEEEAPKKPV 600
           RIY QLGYS +QF  A W++TKWGGLK GQVMA P PVF RIE      +G E A  K V
Sbjct: 541 RIYEQLGYSNEQFGNAAWSDTKWGGLKGGQVMAQPKPVFGRIELKTGTEDGGEPAAAKQV 600

Query: 601 ---KSKKKIAQAVAES 612
              K KK  AQAVAE+
Sbjct: 601 AKKKEKKAKAQAVAEA 607

BLAST of Cucsa.010570 vs. TrEMBL
Match: A0A067L949_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01491 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 4.6e-278
Identity = 486/616 (78.90%), Postives = 536/616 (87.01%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCT-STS 60
           MAARIN S+ T L+ +    S  N   TH +TR+ F  NL  F S S S++A FCT S S
Sbjct: 1   MAARINQSLLTPLYSITR-RSRTN---THVQTRISFPPNL--FLSSSKSRKAAFCTYSIS 60

Query: 61  NFN-SHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTD 120
           N N  +D     + FV+TTPLYYVNA PHMGSAYTTIAADA+ARFQRLLGKKVIF+TGTD
Sbjct: 61  NDNRKNDEAGTAETFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLLGKKVIFVTGTD 120

Query: 121 EHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSK 180
           EHGEKIATAAA  G++PSEHCD+IS+AYKTLWKDL++AYDKFIRTTDPKHEAIVKEFYS+
Sbjct: 121 EHGEKIATAAAACGASPSEHCDVISQAYKTLWKDLEIAYDKFIRTTDPKHEAIVKEFYSR 180

Query: 181 VLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRL 240
           VLANGDIYRADYEGLYCVNCEEYKDEK+LL + CCP+HLKPC  RKEDNYFFALSKYQ+L
Sbjct: 181 VLANGDIYRADYEGLYCVNCEEYKDEKDLLDNKCCPVHLKPCIQRKEDNYFFALSKYQKL 240

Query: 241 LEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDAL 300
           LEETLT+ P FVQP+FRLNEVQSWIRSGLKDFSISRASV+WGIPVPND KQTIYVWFDAL
Sbjct: 241 LEETLTECPNFVQPSFRLNEVQSWIRSGLKDFSISRASVDWGIPVPNDNKQTIYVWFDAL 300

Query: 301 LGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFG 360
           LGYISALSE+ +QPNLQ  V+SGWPASLHLIGKDILRFHAVYWPAMLMSAGL LPKMVFG
Sbjct: 301 LGYISALSEDNKQPNLQSAVSSGWPASLHLIGKDILRFHAVYWPAMLMSAGLGLPKMVFG 360

Query: 361 HGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNA 420
           HGFLTKDG+KMGKSLGNTLEPNEL+EKFG DAVRYFFLREVEFGSDGDY+E+RF+NIVNA
Sbjct: 361 HGFLTKDGLKMGKSLGNTLEPNELIEKFGPDAVRYFFLREVEFGSDGDYSEERFVNIVNA 420

Query: 421 HLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSS 480
           HLANTIGNLLNRTLGLLKKNCQSTLVVD+  AA+GNAFKD VE+LV KAR +YE L+LS 
Sbjct: 421 HLANTIGNLLNRTLGLLKKNCQSTLVVDAAIAAEGNAFKDNVEKLVEKARIHYENLSLSL 480

Query: 481 ACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRL 540
           ACEAVLEIGNAGN YMDER PWSLFK+GGA+S AAAKDLV+ILEAMRIIAIALSPV P L
Sbjct: 481 ACEAVLEIGNAGNGYMDERTPWSLFKKGGAASEAAAKDLVIILEAMRIIAIALSPVAPSL 540

Query: 541 SWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA-PKKP 600
           SWRIYAQLGYS+D F++ TW+ETKWGGLK GQVMA P PVFARIE      + EA  KK 
Sbjct: 541 SWRIYAQLGYSQDHFNSITWSETKWGGLKGGQVMAQPKPVFARIENKTETEDGEATSKKS 600

Query: 601 VKSKKK--IAQAVAES 612
           VK+K+K   AQ VAE+
Sbjct: 601 VKNKEKKPRAQVVAEA 610

BLAST of Cucsa.010570 vs. TAIR10
Match: AT3G55400.1 (AT3G55400.1 methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS))

HSP 1 Score: 928.7 bits (2399), Expect = 1.8e-270
Identity = 455/608 (74.84%), Postives = 521/608 (85.69%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTR-LHFCQNLLSFPSHSSSKRAIFCTSTS 60
           MAARINTS+  +L  L PF++ LN KP  FR     F + L  +   SS KRA++CTS+S
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTKPFSFRRNSFRFSKKLPYYSQFSSGKRALYCTSSS 60

Query: 61  NFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDE 120
             ++ D G   + FV+TTPLYYVNA PHMGSAYTTIAAD++ARFQRLLGKKVIFITGTDE
Sbjct: 61  QESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFITGTDE 120

Query: 121 HGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKV 180
           HGEKIAT+AA  G  P EHCD+IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKEFY++V
Sbjct: 121 HGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEFYARV 180

Query: 181 LANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLL 240
            ANGDIYRADYEGLYCVNCEEYKDEKELL +NCCP+H  PC +RKEDNYFFALSKYQ+ L
Sbjct: 181 FANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKYQKPL 240

Query: 241 EETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALL 300
           E+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D+KQTIYVWFDALL
Sbjct: 241 EDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWFDALL 300

Query: 301 GYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGH 360
           GYISAL+E+ +Q NL+  V+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKMVFGH
Sbjct: 301 GYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKMVFGH 360

Query: 361 GFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAH 420
           GFLTKDGMKMGKSLGNTLEP ELV+KFG DAVRYFFLREVEFG+DGDY+EDRFI IVNAH
Sbjct: 361 GFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKIVNAH 420

Query: 421 LANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSA 480
           LANTIGNLLNRTLGLLKKNC+STLVVDST AA+G   KD VE+LV KAR NYE L+LSSA
Sbjct: 421 LANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSLSSA 480

Query: 481 CEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLS 540
           CEAVLEIGNAGN+YMD+RAPW LFKQGG S+  AAKDLV+ILE MR+IA+ALSPV P LS
Sbjct: 481 CEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVAPCLS 540

Query: 541 WRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVK 600
            RIY+QLGY++DQF++ TW++TKWGGLK GQVM   +PVFARIE   +N E+E  +K  K
Sbjct: 541 LRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIE---LNPEKEEDEKKPK 600

Query: 601 SKKKIAQA 608
             KK  +A
Sbjct: 601 VGKKTGKA 602

BLAST of Cucsa.010570 vs. TAIR10
Match: AT4G13780.1 (AT4G13780.1 methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative)

HSP 1 Score: 148.3 bits (373), Expect = 1.6e-35
Identity = 144/570 (25.26%), Postives = 238/570 (41.75%), Query Frame = 1

Query: 74  VITTPLYYVNAAPHMGSAY-TTIAADAVARFQRLLGKKVIFITGTDEHGEKIATAAATQG 133
           +IT+ L YVN  PH+G+     ++AD  AR+ RL G   I+I GTDE+G    T A  + 
Sbjct: 20  LITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGTDEYGTATETKALEEN 79

Query: 134 STPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKH----EAIV---------------- 193
            TP E CD     +K ++    +++DKF RT+ P+     +AI                 
Sbjct: 80  CTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFNKLWDNKWLSENTMQQ 139

Query: 194 ------KEFYSKVLANGD--IYRADYE---GLYCVNCEEYKDEKELLADNCCPIHLKPCA 253
                 K+F +  L  G       +Y+   G  C  C +  +  EL  D  C +      
Sbjct: 140 LYCDTCKKFLADRLVEGSCPFEGCNYDSARGDQCEKCGKLLNPTEL-KDPKCKVCQNTPR 199

Query: 254 SRKEDNYFFALSKYQRLLEETLTQNPGFVQPAFRLNEVQS---WIRSGLKDFSISRASVE 313
            R  D+ F  L   +  LE  + +    V  ++  N +Q+   W+R GL+   I+R  ++
Sbjct: 200 IRDTDHLFIELPLLKDRLEAYIKKTS--VTGSWSQNAIQTTNAWLRDGLRQRCITR-DLK 259

Query: 314 WGIPVPNDE--KQTIYVWFDALLGYISALSENGEQPNLQEVVASGW------PASLHL-- 373
           WG+PVP+++   +  YVWFDA +GY+S  S             S W      P ++ L  
Sbjct: 260 WGVPVPHEKYKDKVFYVWFDAPIGYVSITS----------CYTSEWEKWWKNPENVELYQ 319

Query: 374 -IGKDILRFHAVYWPAMLMSA--GLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVE- 433
            +GKD + FH V +P+  +       L K +    +L  +  K  KS G  +  N++ + 
Sbjct: 320 FMGKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVFGNDVKDT 379

Query: 434 KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKK--NCQST 493
               +  RY+ L      SD  ++       +N  L + +GN +NR L  + K  N    
Sbjct: 380 NIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAKPDNAGYG 439

Query: 494 LVVDSTAAADGN----AFKDKVEELVVKARNNYEELALSSACEAVLEIGNAGNSYMDERA 553
            V+     A+ +    +  +KVE+ V +     E++ L    +  + I + GN Y+    
Sbjct: 440 SVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGNYYLQASQ 499

Query: 554 PWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYAQLGYS-----KDQF 584
            W L+K+        A  +      + ++A  L P  P  S  ++ QL         D+ 
Sbjct: 500 FWKLYKE---DKPLCAVVIRTAAGLVHLLAQLLEPFMPSFSCEVFKQLNLPPQFSLSDER 559

BLAST of Cucsa.010570 vs. NCBI nr
Match: gi|449449980|ref|XP_004142742.1| (PREDICTED: probable methionine--tRNA ligase, mitochondrial [Cucumis sativus])

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 611/611 (100.00%), Postives = 611/611 (100.00%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 60
           MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 60

Query: 61  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 120
           FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH
Sbjct: 61  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 120

Query: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180
           GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL
Sbjct: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180

Query: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240
           ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE
Sbjct: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240

Query: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 300
           ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG
Sbjct: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 300

Query: 301 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360
           YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG
Sbjct: 301 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360

Query: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420
           FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL
Sbjct: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420

Query: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 480
           ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC
Sbjct: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 480

Query: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540
           EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW
Sbjct: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540

Query: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS 600
           RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS
Sbjct: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS 600

Query: 601 KKKIAQAVAES 612
           KKKIAQAVAES
Sbjct: 601 KKKIAQAVAES 611

BLAST of Cucsa.010570 vs. NCBI nr
Match: gi|659127004|ref|XP_008463474.1| (PREDICTED: LOW QUALITY PROTEIN: probable methionine--tRNA ligase, mitochondrial [Cucumis melo])

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 594/611 (97.22%), Postives = 603/611 (98.69%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 60
           MAARINTSIQTSL LLNPFH+SLNLKPTHFRTRLHF QNLLSF S+SSSKRAIFCT+TSN
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLKPTHFRTRLHFYQNLLSFASYSSSKRAIFCTTTSN 60

Query: 61  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 120
           FNSHD+GAATDPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVI ITGTDEH
Sbjct: 61  FNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILITGTDEH 120

Query: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180
           GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL
Sbjct: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180

Query: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240
           ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE
Sbjct: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240

Query: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 300
           ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND KQTIYVWFDALLG
Sbjct: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDXKQTIYVWFDALLG 300

Query: 301 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360
           YISALSENGEQPNL +VVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG
Sbjct: 301 YISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360

Query: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420
           FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL
Sbjct: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420

Query: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 480
           ANTIGNLLNRTLGLLKKNCQSTL+VDSTAAADGNAFKDKVEELVVKARN+YEELALSSAC
Sbjct: 421 ANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELALSSAC 480

Query: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540
           EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW
Sbjct: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540

Query: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS 600
           RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEE PKKPVKS
Sbjct: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPKKPVKS 600

Query: 601 KKKIAQAVAES 612
           KKKIAQ VAE+
Sbjct: 601 KKKIAQGVAEA 611

BLAST of Cucsa.010570 vs. NCBI nr
Match: gi|1009120595|ref|XP_015877008.1| (PREDICTED: methionine--tRNA ligase, chloroplastic/mitochondrial [Ziziphus jujuba])

HSP 1 Score: 971.8 bits (2511), Expect = 5.4e-280
Identity = 484/611 (79.21%), Postives = 535/611 (87.56%), Query Frame = 1

Query: 3   ARINT-SIQTSLW-LLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 62
           ARIN  ++Q +L   LNP + S N K THFR+RLHF ++ LS    S S++A+FCT+T+N
Sbjct: 4   ARINCYTLQNTLGGFLNPLNHSFNSKATHFRSRLHFHRHHLS----SYSRKAVFCTATAN 63

Query: 63  FNSHDSGAAT----DPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITG 122
               D+  A+    +PFV+TTPLYYVNA PHMGSAYTTIAADA+ARF RLLGK+VIF+TG
Sbjct: 64  TPMKDANEASSSLAEPFVLTTPLYYVNAPPHMGSAYTTIAADAIARFHRLLGKRVIFVTG 123

Query: 123 TDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFY 182
           TDEHGEKIA+AAA  GS P EHCD++S+AYKTLWKDLD+AYDKFIRTTDPKHEAIV+EFY
Sbjct: 124 TDEHGEKIASAAAANGSNPREHCDVVSQAYKTLWKDLDIAYDKFIRTTDPKHEAIVREFY 183

Query: 183 SKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQ 242
           S+VLANGDIYRADYEGLYCVNCEEYKDEKELL +NCCP+HLKPC  RKEDNYFFALSKYQ
Sbjct: 184 SRVLANGDIYRADYEGLYCVNCEEYKDEKELLDNNCCPMHLKPCVPRKEDNYFFALSKYQ 243

Query: 243 RLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFD 302
           +LLEETLTQNP FV+P+FR NEVQSWIRSGLKDFSISRASV+WGIPVPND KQTIYVWFD
Sbjct: 244 KLLEETLTQNPDFVKPSFRQNEVQSWIRSGLKDFSISRASVDWGIPVPNDSKQTIYVWFD 303

Query: 303 ALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV 362
           ALLGYISALSE+ E+PNLQ+ V+SGWPA+LHLIGKDILRFHAVYWPAMLMSAGL LPKMV
Sbjct: 304 ALLGYISALSEDTEEPNLQKAVSSGWPAALHLIGKDILRFHAVYWPAMLMSAGLSLPKMV 363

Query: 363 FGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIV 422
           FGHGFLTKDGMKMGKSLGNT+EPN LV KFGSDAVRYFFLREVEFG DGDY+EDRFINIV
Sbjct: 364 FGHGFLTKDGMKMGKSLGNTIEPNVLVHKFGSDAVRYFFLREVEFGGDGDYSEDRFINIV 423

Query: 423 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELAL 482
           NAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+ AA+G  FKD VE+LV KAR +YE L+ 
Sbjct: 424 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSIAAEGTTFKDDVEKLVEKARVHYENLSP 483

Query: 483 SSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTP 542
           SSACEAVLEIGNAGNSYMDERAPWSLFKQGGA++ AAAKDLV+ILEAMRIIAIALSPV P
Sbjct: 484 SSACEAVLEIGNAGNSYMDERAPWSLFKQGGATAEAAAKDLVIILEAMRIIAIALSPVAP 543

Query: 543 RLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIE-QAIVNGEEEAPK 602
            L  RIY+QLGYSKDQFDAATWNETKWGGLK G V + P PVFARIE Q  V    EA K
Sbjct: 544 SLCCRIYSQLGYSKDQFDAATWNETKWGGLKGGHVTSQPKPVFARIENQTEVGNGFEAAK 603

Query: 603 KPVKSKKKIAQ 607
           K  K K+K+ Q
Sbjct: 604 KVTKRKEKLPQ 610

BLAST of Cucsa.010570 vs. NCBI nr
Match: gi|743844273|ref|XP_011027157.1| (PREDICTED: methionine--tRNA ligase, mitochondrial [Populus euphratica])

HSP 1 Score: 971.1 bits (2509), Expect = 9.2e-280
Identity = 477/607 (78.58%), Postives = 524/607 (86.33%), Query Frame = 1

Query: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 60
           MAARIN S+Q +L LLNPF+ S+N K THF+T ++F +NL+ FP     K  +FCT  ++
Sbjct: 1   MAARINYSVQNTLCLLNPFNYSINAKTTHFKTGINFSKNLI-FPR--KLKSPMFCTCATS 60

Query: 61  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 120
            N   S    + FV+TTPLYYVNA PHMGSAYTTIAADA+ARFQRLLGKKVIF+TGTDEH
Sbjct: 61  NNDLQSSEPAETFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLLGKKVIFVTGTDEH 120

Query: 121 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 180
           GEKIA +AA  GSTPS+HCD+IS+AY+TLWKDL++ YDKFIRTTDPKHE IVKEFYS+VL
Sbjct: 121 GEKIAASAAAAGSTPSDHCDVISQAYRTLWKDLEITYDKFIRTTDPKHEGIVKEFYSRVL 180

Query: 181 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 240
           A GDIYRADYEGLYCVNCEEYKDEKELL +NCCP HLKPC  RKEDNYFFALSKYQ+ LE
Sbjct: 181 AKGDIYRADYEGLYCVNCEEYKDEKELLDNNCCPTHLKPCIERKEDNYFFALSKYQKQLE 240

Query: 241 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 300
           E LT+NP FVQP FRLNEVQSWI+SGLKDFSISRASV+WGIPVPN+ KQTIYVWFDALLG
Sbjct: 241 ENLTRNPKFVQPPFRLNEVQSWIKSGLKDFSISRASVDWGIPVPNNNKQTIYVWFDALLG 300

Query: 301 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 360
           YISALS   EQP+LQ  V+SGWPASLHLIGKDILRFHAVYWPAMLMSAGL LPK VFGHG
Sbjct: 301 YISALSAENEQPDLQTAVSSGWPASLHLIGKDILRFHAVYWPAMLMSAGLDLPKNVFGHG 360

Query: 361 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 420
           FLTKDGMKMGKSLGNTLEPNELV  FGSDAVRYFFLREVEFGSDGDY+E+RFINIVNAHL
Sbjct: 361 FLTKDGMKMGKSLGNTLEPNELVHNFGSDAVRYFFLREVEFGSDGDYSEERFINIVNAHL 420

Query: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 480
           ANTIGNLLNRTLGLLKKNCQSTLVVDS  AA+GNA KD VE+LV KAR +YE L+LSSAC
Sbjct: 421 ANTIGNLLNRTLGLLKKNCQSTLVVDSAVAAEGNALKDTVEKLVEKARIHYENLSLSSAC 480

Query: 481 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 540
           EAVLEIGNAGN+YMDE APW+LFKQGGA+S AAAKDLV+ILEAMRI+AIALSPV P   W
Sbjct: 481 EAVLEIGNAGNAYMDENAPWTLFKQGGAASEAAAKDLVIILEAMRIVAIALSPVAPSFCW 540

Query: 541 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKS 600
           RIY QLGYSKD F+  TW+ TKWGGLK GQVMA P PVFARIE      +E A KKPVKS
Sbjct: 541 RIYEQLGYSKDHFNTVTWSGTKWGGLKGGQVMAQPKPVFARIENKTELEDEAATKKPVKS 600

Query: 601 KKKIAQA 608
           K+K  QA
Sbjct: 601 KQKKPQA 604

BLAST of Cucsa.010570 vs. NCBI nr
Match: gi|359486632|ref|XP_002279143.2| (PREDICTED: probable methionine--tRNA ligase, mitochondrial [Vitis vinifera])

HSP 1 Score: 969.9 bits (2506), Expect = 2.0e-279
Identity = 484/614 (78.83%), Postives = 543/614 (88.44%), Query Frame = 1

Query: 2   AARINTSIQTSLWLLN-PFHSSLNLKPTHFRTRLHFCQNLLSFPSHSSSKRAIFCTSTSN 61
           A R+++SIQ+SL +L+ P +S+      H +TRLHF + L S PS SS  RA+FC+  ++
Sbjct: 3   AVRVHSSIQSSLCILSHPLYSTSR----HLKTRLHFPRPLFSSPSFSSG-RALFCSCRAD 62

Query: 62  FNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGTDEH 121
                    +D FV+TTPLYYVNA PHMGSAYTTIAADA+ARFQRL GKKVIFITGTDEH
Sbjct: 63  NFKGSEREVSDSFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLSGKKVIFITGTDEH 122

Query: 122 GEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVL 181
           GEKIATAAA  GS+PSEHCD+IS+AYKTLW+DLD+AYDKFIRTT+PKHEAIVKEFYS+VL
Sbjct: 123 GEKIATAAAACGSSPSEHCDVISQAYKTLWQDLDIAYDKFIRTTNPKHEAIVKEFYSRVL 182

Query: 182 ANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLE 241
           ANGDIYRADYEGLYC+NCEEYKDEKELL +NCCP+HLKPC  RKEDNYFFALSKYQ+LLE
Sbjct: 183 ANGDIYRADYEGLYCINCEEYKDEKELLENNCCPMHLKPCVLRKEDNYFFALSKYQKLLE 242

Query: 242 ETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLG 301
           ETLTQNP FVQP+FRLNEVQSWI+SGL+DFSISRASV+WGIPVPND KQTIYVWFDALLG
Sbjct: 243 ETLTQNPDFVQPSFRLNEVQSWIKSGLRDFSISRASVDWGIPVPNDNKQTIYVWFDALLG 302

Query: 302 YISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHG 361
           YISALSE+ EQP+LQ  V+SGWPASLHLIGKDILRFHAVYWPAML+SAGL LPKMVFGHG
Sbjct: 303 YISALSEDNEQPDLQSAVSSGWPASLHLIGKDILRFHAVYWPAMLLSAGLSLPKMVFGHG 362

Query: 362 FLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHL 421
           FLTKDGMKMGKSLGNTLEPN+LV++FGSDAVRYFFLREVEFG+DGDY+EDRFINIVNAHL
Sbjct: 363 FLTKDGMKMGKSLGNTLEPNDLVQRFGSDAVRYFFLREVEFGNDGDYSEDRFINIVNAHL 422

Query: 422 ANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALSSAC 481
           ANTIGNLLNRTLGLLKKNCQSTL VDST AA+GNAFKD V++LV KA+  YE L+LSSAC
Sbjct: 423 ANTIGNLLNRTLGLLKKNCQSTLAVDSTLAAEGNAFKDTVDKLVEKAKFQYENLSLSSAC 482

Query: 482 EAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPRLSW 541
           EAVLEIGNAGN Y+DERAPWSLFKQG A+S AAAKDLV+ILEAMRIIAIALSPVTPRLS 
Sbjct: 483 EAVLEIGNAGNLYIDERAPWSLFKQGAAASEAAAKDLVIILEAMRIIAIALSPVTPRLSL 542

Query: 542 RIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIE-QAIVNGEEEAPKKPVK 601
           RIY QLGY+K+QF+A TW+ET+WGGLK+GQVMA P PVFARIE +A V    EA KK VK
Sbjct: 543 RIYEQLGYTKEQFNATTWSETEWGGLKSGQVMAQPKPVFARIEIEAEVGDGGEAAKKVVK 602

Query: 602 SKKKI--AQAVAES 612
            K+KI   Q VA++
Sbjct: 603 KKEKIPHTQGVAQA 611