BLAST of Cucsa.004570 vs. Swiss-Prot
Match:
G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1)
HSP 1 Score: 461.5 bits (1186), Expect = 9.1e-129
Identity = 223/373 (59.79%), Postives = 280/373 (75.07%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDS---IPLIDL 66
++E +++HPVH+ HK+ DF+SL ELP+SY+W D SNN + +S +P+IDL
Sbjct: 4 LSEAYRAHPVHVN-HKHPDFNSLQELPESYNWTHLDDHTLID-SNNIMKESTTTVPVIDL 63
Query: 67 SLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGIS 126
+ PNA +LIG A +TWG +QV+NHG+P+SLL I+ +LFSLP K KA R DG+S
Sbjct: 64 NDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVS 123
Query: 127 GYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLM 186
GYG+ RISSFFPK MW EGFTIVGSPL+HFR+LWP DYTR+CDIV +YD MK L G LM
Sbjct: 124 GYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLM 183
Query: 187 WVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVY 246
+ L LGIT+ED+ WAG F+ + AA QLNSYP CPDPD AMGL HTD++ LTI+
Sbjct: 184 CLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILS 243
Query: 247 QNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSV 306
QN+ GLQV REG+ W+TV P+ G LVV VGDL HIL+NGLYPS +H+ +VNRTR+R SV
Sbjct: 244 QNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQRFSV 303
Query: 307 AYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTGLL 366
AY++GPP + EI P KL+GPT+PPLYR+VTW EYL KA+HFN ALSSVRLC P+ GL
Sbjct: 304 AYLYGPPSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTKAKHFNKALSSVRLCTPINGLF 363
Query: 367 DVNDHSQ--VKVG 375
DVND ++ V+VG
Sbjct: 364 DVNDSNKNSVQVG 374
BLAST of Cucsa.004570 vs. Swiss-Prot
Match:
G3OX1_ARATH (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2)
HSP 1 Score: 424.9 bits (1091), Expect = 9.4e-118
Identity = 204/349 (58.45%), Postives = 252/349 (72.21%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFP-SSSLSNNHLSDSIPLIDLSL 66
+T+VF+ HP+H+P DF SL ELPDSY W D S++ S ++IPLIDL
Sbjct: 5 LTDVFRGHPIHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDH 64
Query: 67 PNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGY 126
P+A IG+A RTWGAFQ+ NHGVP+ LL IE SLF LP KLK+AR G+SGY
Sbjct: 65 PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGY 124
Query: 127 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWV 186
G+ RI+SFF K+MWSEGFTI GSPL FRKLWP + YCDIVEEY+ MK L +LMW+
Sbjct: 125 GVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWL 184
Query: 187 ALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQN 246
AL LG++ ED+ WA + D + AA QLN YPVCP+PDRAMGL AHTD++LLTI+YQN
Sbjct: 185 ALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQN 244
Query: 247 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 306
NT GLQV R+ WVTV P PG+LVV VGDL HIL+NGL+ S +H+A VN+TR RLSVA+
Sbjct: 245 NTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLSVAF 304
Query: 307 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
++GP +ISP+ KL+ P + PLY++VTW EYLR KA HFN ALS +R
Sbjct: 305 LWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353
BLAST of Cucsa.004570 vs. Swiss-Prot
Match:
G3OX2_ARATH (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA3OX2 PE=1 SV=2)
HSP 1 Score: 416.4 bits (1069), Expect = 3.3e-115
Identity = 196/348 (56.32%), Postives = 252/348 (72.41%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLSLP 66
+++VF+SHP+HIP+ DF SL PDSY W D S+ +++ +++PLIDLS
Sbjct: 5 LSDVFRSHPIHIPLSNPPDFKSL---PDSYTWTPKDDLLFSASASD---ETLPLIDLSDI 64
Query: 67 NAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGYG 126
+ L+G+A TWGAFQ+ NHGVP LL IE SLF LP KLKAAR +G+SGYG
Sbjct: 65 HVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYG 124
Query: 127 LVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWVA 186
+ RI+SFF K+MWSEGFT++GSPL FRKLWP + +YC+I+EEY+ M+ L +LMW A
Sbjct: 125 VARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFA 184
Query: 187 LGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQNN 246
LG LG+ +D+ WAGPN DF+ + A QLN YP CP+PDRAMGL AHTD++L+TI+YQNN
Sbjct: 185 LGSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNN 244
Query: 247 TRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYV 306
T GLQV R+ WVT PVPG+LVV VGDLLHILTNG++PS +H+A VN R R S+AY+
Sbjct: 245 TAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYL 304
Query: 307 FGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
+GPP ISPL KL+ P Q PLY ++TW +YL KA HFN +LS +R
Sbjct: 305 WGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346
BLAST of Cucsa.004570 vs. Swiss-Prot
Match:
G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1)
HSP 1 Score: 357.1 bits (915), Expect = 2.4e-97
Identity = 178/339 (52.51%), Postives = 235/339 (69.32%), Query Frame = 1
Query: 28 SLHELPDSYDW--IQPDSF--PSSSLSNNHLSDSIPLIDLSLPNAPQLIGNAFRTWGAFQ 87
SL LP+S+ W DS PSS++S + + +SIP+IDLS P+ LIG+A +TWGAFQ
Sbjct: 15 SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74
Query: 88 VINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGYGLVRISSFFPKRMWSEGF 147
+ NHG+ LL IES + +LF +P KL+AA G+SGYG RIS FF K+MWSEGF
Sbjct: 75 IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134
Query: 148 TIVG-SPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWVALGELGITREDVNWAGP 207
TI S HF LWPHD+T+YC I++EY EM+ L RL++ LG LG+T ED+ WA
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194
Query: 208 --NGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQNNTRGLQVLREGNRWV 267
K A +LN YPVCP+P+RAMGL AHTD+++LTI++Q+NT GLQV RE + WV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254
Query: 268 TVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVF-GPPESAEISPLK 327
TVEP PG LVV +GDL HIL+NG PS VH+A VN TR R+S+AY++ GP +I+P+
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314
Query: 328 KLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP 359
KL GP +P LYR++TW EYL+ K E F+ A+ ++R+ P
Sbjct: 315 KLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRVVNP 353
BLAST of Cucsa.004570 vs. Swiss-Prot
Match:
G3OX3_ARATH (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA3OX3 PE=2 SV=1)
HSP 1 Score: 308.5 bits (789), Expect = 9.8e-83
Identity = 161/355 (45.35%), Postives = 223/355 (62.82%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLSLP 66
+T++FK++PV+ LDF + LPDS+ W +P+ P ++ S IP+I LS P
Sbjct: 4 VTQLFKNNPVNRDRIIPLDFTNTKTLPDSHVWSKPE--PETT------SGPIPVISLSNP 63
Query: 67 NAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGYG 126
L+ A WG F + +HGV SLLH+++ LFSLP K+ A R D +GYG
Sbjct: 64 EEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYG 123
Query: 127 LVRISSFFPKRMWSEGFTIVGSPL-EHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWV 186
+VRIS F+ K MWSEGF+++GS L H LWP D+ +C+++EEY + M L RL+ +
Sbjct: 124 VVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISM 183
Query: 187 ALGELGITREDVNWAGPN---GDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIV 246
+G LG+T ED+ W P+ + + QLNSYPVCPDP AMGL HTD+SLLTI+
Sbjct: 184 LMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTIL 243
Query: 247 YQNNTRGLQV---LREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRK 306
YQ N GL++ EG+RW+ VEP+ G+LVV +GDL HI++NG + S +H+AVVN+T
Sbjct: 244 YQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH 303
Query: 307 RLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
R+S AY GPP++ +I PL PP+YR + W EYL KA HFN AL+ R
Sbjct: 304 RVSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348
BLAST of Cucsa.004570 vs. TrEMBL
Match:
K7ZTU0_CUCSA (Gibberellin 3-oxidase (Fragment) OS=Cucumis sativus GN=ga3ox1 PE=2 SV=1)
HSP 1 Score: 773.5 bits (1996), Expect = 1.2e-220
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 1
Query: 1 MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL 60
MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL
Sbjct: 1 MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL 60
Query: 61 IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD 120
IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD
Sbjct: 61 IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD 120
Query: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCG 180
GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCG
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCG 180
Query: 181 RLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLT 240
RLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLT
Sbjct: 181 RLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLT 240
Query: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR
Sbjct: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
Query: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT
Sbjct: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
Query: 361 GLLDVNDHSQVKVG 375
GLLDVNDHSQVKVG
Sbjct: 361 GLLDVNDHSQVKVG 374
BLAST of Cucsa.004570 vs. TrEMBL
Match:
Q8GT57_CUCMA (Gibberellin 3-oxidase OS=Cucurbita maxima GN=ga3ox PE=2 SV=1)
HSP 1 Score: 657.9 bits (1696), Expect = 7.3e-186
Identity = 318/373 (85.25%), Postives = 342/373 (91.69%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHL-----SDSIPLI 66
I +VFKS PVHIP HKNLDFDSLHELPDSY WIQPDSFPS + +++ SDS+PLI
Sbjct: 5 IADVFKSFPVHIPAHKNLDFDSLHELPDSYAWIQPDSFPSPTHKHHNSILDSDSDSVPLI 64
Query: 67 DLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDG 126
DLSLPNA LIGNAFR+WGAFQVINHGVPISLL SIESSA++LFSLPP HKLKAAR DG
Sbjct: 65 DLSLPNAAALIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAARTPDG 124
Query: 127 ISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGR 186
ISGYGLVRISSFFPKRMWSEGFTIVGSPL+HFR+LWPHDY ++C+IVEEYDREM+SLCGR
Sbjct: 125 ISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMRSLCGR 184
Query: 187 LMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTI 246
LMW+ LGELGITR+D+ WAGP+GDFKTS AATQ NSYPVCPDPDRAMGLG HTDTSLLTI
Sbjct: 185 LMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDTSLLTI 244
Query: 247 VYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRL 306
VYQ+NTRGLQVLREG RWVTVEPV G LVVQVGDLLHILTNGLYPS +HQAVVNRTRKRL
Sbjct: 245 VYQSNTRGLQVLREGKRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNRTRKRL 304
Query: 307 SVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTG 366
SVAYVFGPPESAEISPLKKLLGPTQPPLYR VTWTEYL KKAEHFNNALS+VRLCAP+TG
Sbjct: 305 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKKAEHFNNALSTVRLCAPITG 364
Query: 367 LLDVNDHSQVKVG 375
LLDVNDHS+VKVG
Sbjct: 365 LLDVNDHSRVKVG 377
BLAST of Cucsa.004570 vs. TrEMBL
Match:
S0BE27_CUCSA (GA 3-oxidase OS=Cucumis sativus GN=GA3ox3 PE=2 SV=1)
HSP 1 Score: 608.6 bits (1568), Expect = 5.1e-171
Identity = 290/368 (78.80%), Postives = 326/368 (88.59%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLSL 65
K+T+++K+HP+ IP +K L+ DSL +LPD+YDWIQPD+FPS N LSDS+PLIDLSL
Sbjct: 8 KLTDIYKTHPLDIPTNK-LNLDSLQQLPDTYDWIQPDAFPSFRDPNISLSDSVPLIDLSL 67
Query: 66 PNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGY 125
PNAP LIGNA +TWG FQVINHGVPISLL+S+ES N+LF LP PHKLKAAR DG++GY
Sbjct: 68 PNAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNTLFDLPSPHKLKAARTPDGVTGY 127
Query: 126 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWV 185
GL RIS+FFPKRMWSEGFTIVGSPLEHF+KLWP++Y++YCDI EEY REMKSLCGR++W+
Sbjct: 128 GLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPNEYSKYCDITEEYSREMKSLCGRVIWL 187
Query: 186 ALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQN 245
ALGELGITREDVNWA NGDFKTSNA QLNSYPVCPDPDRAMGLGAHTDTSLLTI+YQN
Sbjct: 188 ALGELGITREDVNWAEANGDFKTSNAVIQLNSYPVCPDPDRAMGLGAHTDTSLLTILYQN 247
Query: 246 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 305
NTRGLQVLREGNRWV VEPV GALVVQVGDLLHILTNGLYPS HQAVVN+ RKR+S+AY
Sbjct: 248 NTRGLQVLREGNRWVMVEPVTGALVVQVGDLLHILTNGLYPSSAHQAVVNQNRKRISIAY 307
Query: 306 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTGLLDV 365
FGP ESAEISPL KL+ PTQP LY TVTWTEYLRKKA+ FNN LSS+RL APLTG LD+
Sbjct: 308 FFGPSESAEISPLNKLVTPTQPLLYPTVTWTEYLRKKAQLFNNTLSSIRLSAPLTGALDI 367
Query: 366 NDHSQVKV 374
NDH+QVKV
Sbjct: 368 NDHNQVKV 374
BLAST of Cucsa.004570 vs. TrEMBL
Match:
E1AXG5_9ROSI (Gibberellin 3-oxidase 2 (Fragment) OS=Marah macrocarpus PE=2 SV=1)
HSP 1 Score: 601.7 bits (1550), Expect = 6.2e-169
Identity = 286/370 (77.30%), Postives = 323/370 (87.30%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLSL 65
KIT+ FKSHPVH+P HKNLDFDS +ELPD+Y+W+QPDS PS N+ + +PLIDLSL
Sbjct: 4 KITDAFKSHPVHVPAHKNLDFDSFNELPDTYEWVQPDSIPSP---NDQSQEHLPLIDLSL 63
Query: 66 PNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGY 125
PNA L+G+AFR+WGAFQ INHGVPISLLHS ES+ ++LFSLPP K KAAR DG+ GY
Sbjct: 64 PNAAALVGDAFRSWGAFQAINHGVPISLLHSFESTVDTLFSLPPSDKHKAARTPDGVDGY 123
Query: 126 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWV 185
GLVRISSFFPKRMWSEGFTI+ SPL HF+KLWP DY ++C+I EEYDREM+SLC RL+W+
Sbjct: 124 GLVRISSFFPKRMWSEGFTILNSPLHHFQKLWPDDYAKHCEIYEEYDREMRSLCARLIWL 183
Query: 186 ALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQN 245
LGELGIT++DV AGP+ DFKT+ AATQLNSYP+CPDP+RAMGLGAHTDTSLLTIVYQN
Sbjct: 184 GLGELGITQDDVELAGPDRDFKTNPAATQLNSYPICPDPERAMGLGAHTDTSLLTIVYQN 243
Query: 246 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 305
NT GLQ LREGNRWVTVEPV GALVVQVGDLL I+TNGLY SP+HQA VNRTRKRLS+AY
Sbjct: 244 NTSGLQFLREGNRWVTVEPVDGALVVQVGDLLQIMTNGLYKSPLHQAFVNRTRKRLSMAY 303
Query: 306 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP-LTGLLD 365
+FG PE+AEISPLKKLLGPTQPP+YRTVTWTEYL KKAEHFNNAL SVR+CAP GLLD
Sbjct: 304 IFGTPETAEISPLKKLLGPTQPPIYRTVTWTEYLGKKAEHFNNALKSVRICAPSANGLLD 363
Query: 366 VNDHSQVKVG 375
VND SQVKVG
Sbjct: 364 VNDQSQVKVG 370
BLAST of Cucsa.004570 vs. TrEMBL
Match:
A0A0A0KAA7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G435470 PE=3 SV=1)
HSP 1 Score: 549.3 bits (1414), Expect = 3.7e-153
Identity = 266/375 (70.93%), Postives = 304/375 (81.07%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPS-------SSLSNNHLSDSI 65
K+ E +K+ PVH +KNLD S ++PDS+DW+QP+SFPS S LS++ +DS+
Sbjct: 6 KMIEAYKTDPVHALTNKNLD--SFEQIPDSHDWVQPNSFPSFTDQSNISDLSSD--TDSV 65
Query: 66 PLIDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARP 125
PLIDLSLPNAP+LIGNA RTWG FQ INHGVPIS+L+S+ES N LF LP P KLKAAR
Sbjct: 66 PLIDLSLPNAPKLIGNALRTWGVFQAINHGVPISVLNSMESLLNDLFDLPTPQKLKAARS 125
Query: 126 SDGISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSL 185
DG++GYG RIS+FFPK MWSEGFT+ GSPLEHF+ LWPHD T+YCDI+EEYDREMK L
Sbjct: 126 RDGVNGYGRFRISTFFPKSMWSEGFTVSGSPLEHFQILWPHDCTKYCDIIEEYDREMKGL 185
Query: 186 CGRLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSL 245
CGR++W+ALGELGITREDVNWAGPNGDF TS+ LNSYPVCPDPDRAMG+G HTD+
Sbjct: 186 CGRVVWLALGELGITREDVNWAGPNGDFPTSSGVMNLNSYPVCPDPDRAMGIGVHTDSCF 245
Query: 246 LTIVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTR 305
LT++YQNN GLQVLREG RWVTV+PVPGALVVQV DLL ILTNGLY SP HQAVVNR R
Sbjct: 246 LTLLYQNNASGLQVLREGKRWVTVDPVPGALVVQVADLLQILTNGLYSSPFHQAVVNRDR 305
Query: 306 KRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP 365
KRLSVAY FGPP AEISP+KKL+ PTQP LY TVTW EYL KKAE FN+ L S+RL P
Sbjct: 306 KRLSVAYFFGPPVHAEISPIKKLVNPTQPLLYPTVTWAEYLCKKAELFNDTLPSIRLSTP 365
Query: 366 LTGLLDVNDHSQVKV 374
T DVNDHSQVK+
Sbjct: 366 PTESSDVNDHSQVKI 376
BLAST of Cucsa.004570 vs. TAIR10
Match:
AT1G15550.1 (AT1G15550.1 gibberellin 3-oxidase 1)
HSP 1 Score: 424.9 bits (1091), Expect = 5.3e-119
Identity = 204/349 (58.45%), Postives = 252/349 (72.21%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFP-SSSLSNNHLSDSIPLIDLSL 66
+T+VF+ HP+H+P DF SL ELPDSY W D S++ S ++IPLIDL
Sbjct: 5 LTDVFRGHPIHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDH 64
Query: 67 PNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGY 126
P+A IG+A RTWGAFQ+ NHGVP+ LL IE SLF LP KLK+AR G+SGY
Sbjct: 65 PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGY 124
Query: 127 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWV 186
G+ RI+SFF K+MWSEGFTI GSPL FRKLWP + YCDIVEEY+ MK L +LMW+
Sbjct: 125 GVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWL 184
Query: 187 ALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQN 246
AL LG++ ED+ WA + D + AA QLN YPVCP+PDRAMGL AHTD++LLTI+YQN
Sbjct: 185 ALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQN 244
Query: 247 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 306
NT GLQV R+ WVTV P PG+LVV VGDL HIL+NGL+ S +H+A VN+TR RLSVA+
Sbjct: 245 NTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLSVAF 304
Query: 307 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
++GP +ISP+ KL+ P + PLY++VTW EYLR KA HFN ALS +R
Sbjct: 305 LWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353
BLAST of Cucsa.004570 vs. TAIR10
Match:
AT1G80340.1 (AT1G80340.1 gibberellin 3-oxidase 2)
HSP 1 Score: 416.4 bits (1069), Expect = 1.9e-116
Identity = 196/348 (56.32%), Postives = 252/348 (72.41%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLSLP 66
+++VF+SHP+HIP+ DF SL PDSY W D S+ +++ +++PLIDLS
Sbjct: 5 LSDVFRSHPIHIPLSNPPDFKSL---PDSYTWTPKDDLLFSASASD---ETLPLIDLSDI 64
Query: 67 NAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGYG 126
+ L+G+A TWGAFQ+ NHGVP LL IE SLF LP KLKAAR +G+SGYG
Sbjct: 65 HVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYG 124
Query: 127 LVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWVA 186
+ RI+SFF K+MWSEGFT++GSPL FRKLWP + +YC+I+EEY+ M+ L +LMW A
Sbjct: 125 VARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFA 184
Query: 187 LGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQNN 246
LG LG+ +D+ WAGPN DF+ + A QLN YP CP+PDRAMGL AHTD++L+TI+YQNN
Sbjct: 185 LGSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNN 244
Query: 247 TRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYV 306
T GLQV R+ WVT PVPG+LVV VGDLLHILTNG++PS +H+A VN R R S+AY+
Sbjct: 245 TAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYL 304
Query: 307 FGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
+GPP ISPL KL+ P Q PLY ++TW +YL KA HFN +LS +R
Sbjct: 305 WGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346
BLAST of Cucsa.004570 vs. TAIR10
Match:
AT1G80330.1 (AT1G80330.1 gibberellin 3-oxidase 4)
HSP 1 Score: 357.1 bits (915), Expect = 1.4e-98
Identity = 178/339 (52.51%), Postives = 235/339 (69.32%), Query Frame = 1
Query: 28 SLHELPDSYDW--IQPDSF--PSSSLSNNHLSDSIPLIDLSLPNAPQLIGNAFRTWGAFQ 87
SL LP+S+ W DS PSS++S + + +SIP+IDLS P+ LIG+A +TWGAFQ
Sbjct: 15 SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74
Query: 88 VINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGYGLVRISSFFPKRMWSEGF 147
+ NHG+ LL IES + +LF +P KL+AA G+SGYG RIS FF K+MWSEGF
Sbjct: 75 IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134
Query: 148 TIVG-SPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWVALGELGITREDVNWAGP 207
TI S HF LWPHD+T+YC I++EY EM+ L RL++ LG LG+T ED+ WA
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194
Query: 208 --NGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQNNTRGLQVLREGNRWV 267
K A +LN YPVCP+P+RAMGL AHTD+++LTI++Q+NT GLQV RE + WV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254
Query: 268 TVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVF-GPPESAEISPLK 327
TVEP PG LVV +GDL HIL+NG PS VH+A VN TR R+S+AY++ GP +I+P+
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314
Query: 328 KLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP 359
KL GP +P LYR++TW EYL+ K E F+ A+ ++R+ P
Sbjct: 315 KLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRVVNP 353
BLAST of Cucsa.004570 vs. TAIR10
Match:
AT4G21690.1 (AT4G21690.1 gibberellin 3-oxidase 3)
HSP 1 Score: 308.5 bits (789), Expect = 5.5e-84
Identity = 161/355 (45.35%), Postives = 223/355 (62.82%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLSLP 66
+T++FK++PV+ LDF + LPDS+ W +P+ P ++ S IP+I LS P
Sbjct: 4 VTQLFKNNPVNRDRIIPLDFTNTKTLPDSHVWSKPE--PETT------SGPIPVISLSNP 63
Query: 67 NAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGYG 126
L+ A WG F + +HGV SLLH+++ LFSLP K+ A R D +GYG
Sbjct: 64 EEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYG 123
Query: 127 LVRISSFFPKRMWSEGFTIVGSPL-EHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWV 186
+VRIS F+ K MWSEGF+++GS L H LWP D+ +C+++EEY + M L RL+ +
Sbjct: 124 VVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISM 183
Query: 187 ALGELGITREDVNWAGPN---GDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIV 246
+G LG+T ED+ W P+ + + QLNSYPVCPDP AMGL HTD+SLLTI+
Sbjct: 184 LMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTIL 243
Query: 247 YQNNTRGLQV---LREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRK 306
YQ N GL++ EG+RW+ VEP+ G+LVV +GDL HI++NG + S +H+AVVN+T
Sbjct: 244 YQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH 303
Query: 307 RLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
R+S AY GPP++ +I PL PP+YR + W EYL KA HFN AL+ R
Sbjct: 304 RVSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348
BLAST of Cucsa.004570 vs. TAIR10
Match:
AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 179.9 bits (455), Expect = 3.0e-45
Identity = 108/305 (35.41%), Postives = 157/305 (51.48%), Query Frame = 1
Query: 47 SSLSNNHLSDSIPLIDLSLPNAPQ---------LIGNAFRTWGAFQVINHGVPISLLHSI 106
SSL +HL IP+IDLS + P + A WG FQVINHG+ + ++ I
Sbjct: 44 SSLKTHHLHHQIPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDI 103
Query: 107 ESSANSLFSLPPPHKLKAARPSDGISGYGLVRISSFFPKRMWSEGFTI-VGSPLEHFRKL 166
E A+ F +P K K + GYG I S K W F + V P KL
Sbjct: 104 EEVASEFFDMPLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKL 163
Query: 167 WPHDYTRYCDIVEEYDREMKSLCGRLMWVALGELGITREDVNWAGPNGDFKTSNAATQLN 226
WP R+ + +E Y +E++ LC RL+ LG+ E F + A ++N
Sbjct: 164 WPSKPARFSESLEGYSKEIRELCKRLLKYIAISLGLKEERFEEM-----FGEAVQAVRMN 223
Query: 227 SYPVCPDPDRAMGLGAHTDTSLLTIVYQ--NNTRGLQVLREGNRWVTVEPVPGALVVQVG 286
YP C PD +GL H+D S LT++ Q N+ GLQ+L++ N WV V+P+P ALV+ +G
Sbjct: 224 YYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSCVGLQILKD-NTWVPVKPLPNALVINIG 283
Query: 287 DLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLL-GPTQPPLYRTV 339
D + +L+NG Y S H+AV NR ++RL++ + P EI P+ +L+ T P YR+
Sbjct: 284 DTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSY 342
BLAST of Cucsa.004570 vs. NCBI nr
Match:
gi|449436096|ref|XP_004135830.1| (PREDICTED: gibberellin 3-beta-dioxygenase 1 [Cucumis sativus])
HSP 1 Score: 773.5 bits (1996), Expect = 1.7e-220
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 1
Query: 1 MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL 60
MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL
Sbjct: 1 MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL 60
Query: 61 IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD 120
IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD
Sbjct: 61 IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD 120
Query: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCG 180
GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCG
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCG 180
Query: 181 RLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLT 240
RLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLT
Sbjct: 181 RLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLT 240
Query: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR
Sbjct: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
Query: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT
Sbjct: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
Query: 361 GLLDVNDHSQVKVG 375
GLLDVNDHSQVKVG
Sbjct: 361 GLLDVNDHSQVKVG 374
BLAST of Cucsa.004570 vs. NCBI nr
Match:
gi|659122279|ref|XP_008461056.1| (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])
HSP 1 Score: 755.7 bits (1950), Expect = 3.7e-215
Identity = 365/374 (97.59%), Postives = 368/374 (98.40%), Query Frame = 1
Query: 1 MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL 60
MGSNIKITEVFKSHPVHIPIHKN DFDSL ELPDSYDWIQPDSFP+SSLSNNHLSDSIPL
Sbjct: 1 MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL 60
Query: 61 IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD 120
IDLSLPNAP LIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLP PHKLKAARPSD
Sbjct: 61 IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD 120
Query: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCG 180
GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYD+EMKSLCG
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCG 180
Query: 181 RLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLT 240
RLMWVALGELGITREDVNWAGPNGDF TSNAATQ NSYPVCPDP+RAMGLGAHTDTSLLT
Sbjct: 181 RLMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLT 240
Query: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR
Sbjct: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
Query: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT
Sbjct: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
Query: 361 GLLDVNDHSQVKVG 375
GLLDVNDHSQVKVG
Sbjct: 361 GLLDVNDHSQVKVG 374
BLAST of Cucsa.004570 vs. NCBI nr
Match:
gi|27123661|emb|CAC83089.1| (gibberellin 3-oxidase [Cucurbita maxima])
HSP 1 Score: 657.9 bits (1696), Expect = 1.1e-185
Identity = 318/373 (85.25%), Postives = 342/373 (91.69%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHL-----SDSIPLI 66
I +VFKS PVHIP HKNLDFDSLHELPDSY WIQPDSFPS + +++ SDS+PLI
Sbjct: 5 IADVFKSFPVHIPAHKNLDFDSLHELPDSYAWIQPDSFPSPTHKHHNSILDSDSDSVPLI 64
Query: 67 DLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDG 126
DLSLPNA LIGNAFR+WGAFQVINHGVPISLL SIESSA++LFSLPP HKLKAAR DG
Sbjct: 65 DLSLPNAAALIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAARTPDG 124
Query: 127 ISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGR 186
ISGYGLVRISSFFPKRMWSEGFTIVGSPL+HFR+LWPHDY ++C+IVEEYDREM+SLCGR
Sbjct: 125 ISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMRSLCGR 184
Query: 187 LMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTI 246
LMW+ LGELGITR+D+ WAGP+GDFKTS AATQ NSYPVCPDPDRAMGLG HTDTSLLTI
Sbjct: 185 LMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDTSLLTI 244
Query: 247 VYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRL 306
VYQ+NTRGLQVLREG RWVTVEPV G LVVQVGDLLHILTNGLYPS +HQAVVNRTRKRL
Sbjct: 245 VYQSNTRGLQVLREGKRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNRTRKRL 304
Query: 307 SVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTG 366
SVAYVFGPPESAEISPLKKLLGPTQPPLYR VTWTEYL KKAEHFNNALS+VRLCAP+TG
Sbjct: 305 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKKAEHFNNALSTVRLCAPITG 364
Query: 367 LLDVNDHSQVKVG 375
LLDVNDHS+VKVG
Sbjct: 365 LLDVNDHSRVKVG 377
BLAST of Cucsa.004570 vs. NCBI nr
Match:
gi|525507174|ref|NP_001267625.1| (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus])
HSP 1 Score: 608.6 bits (1568), Expect = 7.3e-171
Identity = 290/368 (78.80%), Postives = 326/368 (88.59%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLSL 65
K+T+++K+HP+ IP +K L+ DSL +LPD+YDWIQPD+FPS N LSDS+PLIDLSL
Sbjct: 8 KLTDIYKTHPLDIPTNK-LNLDSLQQLPDTYDWIQPDAFPSFRDPNISLSDSVPLIDLSL 67
Query: 66 PNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGY 125
PNAP LIGNA +TWG FQVINHGVPISLL+S+ES N+LF LP PHKLKAAR DG++GY
Sbjct: 68 PNAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNTLFDLPSPHKLKAARTPDGVTGY 127
Query: 126 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWV 185
GL RIS+FFPKRMWSEGFTIVGSPLEHF+KLWP++Y++YCDI EEY REMKSLCGR++W+
Sbjct: 128 GLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPNEYSKYCDITEEYSREMKSLCGRVIWL 187
Query: 186 ALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQN 245
ALGELGITREDVNWA NGDFKTSNA QLNSYPVCPDPDRAMGLGAHTDTSLLTI+YQN
Sbjct: 188 ALGELGITREDVNWAEANGDFKTSNAVIQLNSYPVCPDPDRAMGLGAHTDTSLLTILYQN 247
Query: 246 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 305
NTRGLQVLREGNRWV VEPV GALVVQVGDLLHILTNGLYPS HQAVVN+ RKR+S+AY
Sbjct: 248 NTRGLQVLREGNRWVMVEPVTGALVVQVGDLLHILTNGLYPSSAHQAVVNQNRKRISIAY 307
Query: 306 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTGLLDV 365
FGP ESAEISPL KL+ PTQP LY TVTWTEYLRKKA+ FNN LSS+RL APLTG LD+
Sbjct: 308 FFGPSESAEISPLNKLVTPTQPLLYPTVTWTEYLRKKAQLFNNTLSSIRLSAPLTGALDI 367
Query: 366 NDHSQVKV 374
NDH+QVKV
Sbjct: 368 NDHNQVKV 374
BLAST of Cucsa.004570 vs. NCBI nr
Match:
gi|304636273|gb|ADM47338.1| (gibberellin 3-oxidase 2 [Marah macrocarpus])
HSP 1 Score: 601.7 bits (1550), Expect = 9.0e-169
Identity = 286/370 (77.30%), Postives = 323/370 (87.30%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLSL 65
KIT+ FKSHPVH+P HKNLDFDS +ELPD+Y+W+QPDS PS N+ + +PLIDLSL
Sbjct: 4 KITDAFKSHPVHVPAHKNLDFDSFNELPDTYEWVQPDSIPSP---NDQSQEHLPLIDLSL 63
Query: 66 PNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISGY 125
PNA L+G+AFR+WGAFQ INHGVPISLLHS ES+ ++LFSLPP K KAAR DG+ GY
Sbjct: 64 PNAAALVGDAFRSWGAFQAINHGVPISLLHSFESTVDTLFSLPPSDKHKAARTPDGVDGY 123
Query: 126 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMWV 185
GLVRISSFFPKRMWSEGFTI+ SPL HF+KLWP DY ++C+I EEYDREM+SLC RL+W+
Sbjct: 124 GLVRISSFFPKRMWSEGFTILNSPLHHFQKLWPDDYAKHCEIYEEYDREMRSLCARLIWL 183
Query: 186 ALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQN 245
LGELGIT++DV AGP+ DFKT+ AATQLNSYP+CPDP+RAMGLGAHTDTSLLTIVYQN
Sbjct: 184 GLGELGITQDDVELAGPDRDFKTNPAATQLNSYPICPDPERAMGLGAHTDTSLLTIVYQN 243
Query: 246 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 305
NT GLQ LREGNRWVTVEPV GALVVQVGDLL I+TNGLY SP+HQA VNRTRKRLS+AY
Sbjct: 244 NTSGLQFLREGNRWVTVEPVDGALVVQVGDLLQIMTNGLYKSPLHQAFVNRTRKRLSMAY 303
Query: 306 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP-LTGLLD 365
+FG PE+AEISPLKKLLGPTQPP+YRTVTWTEYL KKAEHFNNAL SVR+CAP GLLD
Sbjct: 304 IFGTPETAEISPLKKLLGPTQPPIYRTVTWTEYLGKKAEHFNNALKSVRICAPSANGLLD 363
Query: 366 VNDHSQVKVG 375
VND SQVKVG
Sbjct: 364 VNDQSQVKVG 370
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
G3OX_PEA | 9.1e-129 | 59.79 | Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1 | [more] |
G3OX1_ARATH | 9.4e-118 | 58.45 | Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2 | [more] |
G3OX2_ARATH | 3.3e-115 | 56.32 | Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA3OX2 PE=1 SV=2 | [more] |
G3OX4_ARATH | 2.4e-97 | 52.51 | Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1 | [more] |
G3OX3_ARATH | 9.8e-83 | 45.35 | Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA3OX3 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
K7ZTU0_CUCSA | 1.2e-220 | 100.00 | Gibberellin 3-oxidase (Fragment) OS=Cucumis sativus GN=ga3ox1 PE=2 SV=1 | [more] |
Q8GT57_CUCMA | 7.3e-186 | 85.25 | Gibberellin 3-oxidase OS=Cucurbita maxima GN=ga3ox PE=2 SV=1 | [more] |
S0BE27_CUCSA | 5.1e-171 | 78.80 | GA 3-oxidase OS=Cucumis sativus GN=GA3ox3 PE=2 SV=1 | [more] |
E1AXG5_9ROSI | 6.2e-169 | 77.30 | Gibberellin 3-oxidase 2 (Fragment) OS=Marah macrocarpus PE=2 SV=1 | [more] |
A0A0A0KAA7_CUCSA | 3.7e-153 | 70.93 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G435470 PE=3 SV=1 | [more] |