CsaV3_7G000530 (gene) Cucumber (Chinese Long) v3

NameCsaV3_7G000530
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
DescriptiontRNA-dihydrouridine(47) synthase [NAD(P)(+)]
Locationchr7 : 592559 .. 598379 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTACATTAAAATTGATTTAAAATGGAAGTGAAAGAGAAGTTGAACCCTAGCAATTAGCATGGTCTTAAGTCCAACTTCTTCAAAATCCTTTGACGTAACTTCCCACTTTTCCCAATTTCCATATCTTTCAGTTACTACTTCTCATTCGCCCTAGCACACTCCTCACCAATGGAAGAAGACTCGGTTACGGCGGACCTCCTCGACGACACCGCGGTCTCCGAGCACCCTCTTTCTCACTCATCCGACAACGGTACTCACTTGACGCCTCAACAACTAGTAGCCAAGGCCATAGCTCCCGTTAAGCGTGACTTTCTTCGCCCTCCGCCTCTCAGGTCATCCTCTAACAACCTTAAGGATGCTACTTCCACTGCTAAGAATGATGGTGATGATAAAGCTGCTCCCTCCACCTTGGTCAAGGAGAAGAAGTCCAAACGTCAATTGAAACGTGAACGCCGTCAGGTTAACTTCTCATTCATATCGCCTTTTGTTCTTGTATTACACTTCTGTTCCTTAACTTGCGTTATTTTAAGATGAATTGAATGTAGTTTGGAGTATAACCTTGTTTTCTGGAATTGATGGTGTACGGTTGATTTGCACAATGTAAAATACTTGCAAACGTTTGTGATGAGTATCACTGACTTCAACTCGGCCATGTATATATATATACGATTTCATGAACACGAATTGTTCAAAGTATAAATTATATAGTCTCTTCGATGAGTACAGATTATAACTATCGTACTTTACAGGAACAGAAGTCCACTCGTCACTTGTGTCCTGAAATAGCGAAGACAGGAGATGTTAGTTCATGTCGGTATAGTGATAAATGCCGATTTAGTCATGATATTGAAGCCTTTAAATCTCAGGTAGTTTATTGTATATCTCACTTCTAGCCAACTTTTGGTCTAACAAATTTTCTTTTCTCAAAGTAATTCGTGATTTTACCTTTATTTTTGACTAGAGGTTTGTGATCTCAGAAACCAGCTGATCTCGAAGGAGAGTGTCCATTCAGTGGTGCTGAACAACTTTGCCCTTACGGTTTAGCGTGTAGATTTGCGGGGACCCATAAGGAAGGTGTTCCTGCAGGTTCTTCAGTTACTTTGAAGAGATGCTCTGAAATGAATGGATTGAATAAAGATATTCAGAAGCTCTTATGGAAAAATAAGATGAAATTTCCCAAAGCTGATTCCAAACTCAAAGAACTTGGGCTCTTGGTATGACCAGAAAGCATTGTCTATCTTTTTTAGATCTTTTTCTTTTTCATGGGATCTGACATGCTATTTTGAGTTCTTGCGACACAATGTTACTTAAATCATGCATCATTTTGAGATAAGGGGTGAAGAGGCTATAGCTTTCAAGGAATCAACTCTAATGTAGTCTGTTTCCATTGCATAATTATTCATTGATGTAATCGTGGTGCTGCTAGGGGCATGGAAAGTCAAAATTAAGGGTCAAAGAAGATGAGGAAAATGCTGTATCGAATGACAGTGTGGTTGCGGACAGACCATGCTGCAGTGAAATGAACAATGACTTGGATGCTGACTTAGAGATCTCTGTGGAAGTGCCAGAAGAAAATGGGGAGAAAGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAATCAAAATCTTTGTCTTCAGAGAATTTTCATTCTGATCAAATTGACAATGGTGGTTTACATTTTATTTCTTTTTTCTGTTTATTTTCGTCCGGAGATGTTTAAGTTCAACTAGCCCTTTATTCTATAGTTTCATCTTCCTTCTGTTATTAATACTCTCATGTGTGCAACAGGGGCATGTATGATTGATGAAACTATCGGTGAGGGCCATGCGCCAATCGAATCTGAAGCTATGAACGACAGTGTACTTCTTGATAGTGATGCAAGTCTAAAACCACATCCACGTGAAAAAAAGCTTATTGATTTTAGAGATAAGCTGTATCTCGCTCCTCTGACCACTGTCGGTAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGACGTGACATGCGGTGAAATGGCAATGTGTACAAATCTATTGCAGGTTGTGCTCCCTTGGCTCTGCAGAAATTAATATCAATAAAGGGTCCTTAATTGATTTGAATCAGCTAGCAACTGAGTTCCAAATTATTGGTTTTAAAAATAATTTGTATGTTCTCATACAATGAATCATTGTATGTTTATGATAAGGCCCACCGGTTAACCATTTAGTAGTCTTATGATAATATGCAAGTATAGTTCCAGATAGAGTTATGTTAAAGATGGTTTGGCTTTTTTCATTTTAATCTAAAGTACACCTTATTTAGCATGTTTCTGGTTTAATAATTTTGTGATGATCTTCCCATTTCATTTTTGTAAGTACAATTAAATTAAACCTTCTTTTCATATTTACTTATATGGTATAGGGTCAAGCATCAGAATGGGCATTGTTGAGACGACATTCTTCAGAAGATCTATTTGGTGTACAGATATGTGGTGCATATCCAGACACTGTTTCGCGTGCTGTAGAACTTATAGACAAAGAATGCGTGCTTGACTTCATTGACATAAACATGGGATGCCCAATTGATATTGTCGTGAATAAGGGTGCTGGATCAGCTCTTCTTACAAAGCCAATGCGTATGAAAACTGTTGTAGAAGCAGCTTCGGGAATTGTGGATACACCCATAACAATTAAGGTATTGAGTGTCTTTATGTACATGTTTCATTTTCAATGGAATGACCAATATTGACTGGTGAAGTTGCTTTTAACTGAAGTTATGGGCAACCTCCTTACATTTCTCTTTTTCTTCCTTTGCCCTAAATTTATTGCATGAATTAGTGATGGTCTCATAGGTAAGTTTAGGAGAGAAAAGTTAAAACAGAAAATGAGACGGACAAACATTTTGGAGAATTTAAGAGGCTAAAATTTAATGGAGGATTAGCATTTGTAATGTGGGGAAAAATGTAAAATCATTCAATCATAGATTTACAAAGCATTGAGACGAAACCTGCAGTTAAATGAATGTCTGATAGACGTTCCCTCCAATGTATCAAAATTTGAGACTTTTTTAGATAAAAATTAAAAAAGAAGGATATGATTGAATATTTGACAACCTTGTTTCACGTTCACAGTATCATCAATTCTCCATGTATCAAAATTTGAGACTTGAATAGATATGATTTAAAAAATTTGTGTTGGTTTGCATTAGGAATTTATGATAAACAAAAAAATCAGTTCTGTATGGTATTGGATAGTGTATTGTTGATTTTGAATGACCAGCATGTTCTCTTTTAGGGGAGAGGGGAAGGGGAACTGTTATTACTTAGGTTGGTAATCTACATTTTGGTTAATTTTCACCAAGTTTTTCAATTTGCATAATGTAACTAGGTTCGGACAGGTTACTATGAAGGGAAAAACCGTATCGATTCGCTTATTGCAGATATTGGTAACTGGGGAGCAAGTGCTGTAACCATTCATGGTCGGACTCGTCAGCAACGATACAGCAAGCTTGCTGACTGGGAATACATCAATCGGTGTTCCTCGTTGGCTCCTGATTCTTTGCAAGTTATAGGAAACGGGGATGTATTTTCATATACAGATTGGAATGAACACAAGAATGAATGCCCAAAGTTAGCCACATGCATGATCGCTAGAGGAGCACTAATTAAGGTCTGCAAGTCAAGCTCTTTTGTTTATGACATTCTCTTTTCGTATGATTTTCTGTCTCAACCTTCAACTCAGTTGGGGAAGGATTAAAATATCAATATTTATGTCAATATCGATATATCAATTTTATGCCCTATCATTATCTACTAGTATTTATCTAAATCATAAAAATGATTTATACTAATAAATAAGTTGTTTTGAATTCCAAACTAAGTTGTAGACCTTATTATTAGTATTTATAATTATTATGGAATTAATAGATGGTGCTTTTTTAAAACTTTACTTTATGGATACTGAGAAAAGATATCCTCTCTGATTTATGGATATATCAGATGGAATTTTTGAGATATCGACCAATATTTTTATCCTTTTTGAAGCCATACTGAGCAACAACGCATGCATGCATAGATTATAAAAATAAAAAAAATAAATATATAATTAAAAAACTAGAACAATTTGTAAATGTTTTTCGAATTACTTGTTTTGTAAATATTCTTAAAAACCACCCCTATTTTCACACTAAATCCCTAATGTTCATATCATCTGTTGTCCTTTGCAGCCTTGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATCACATCAGGGGAGCGGCTAAACATCTTGAAGGATTTTGCTCACTTTGGACTTGAGCATTGGGGATCCGATTCCAAAGGTATGTCATTTATTGCTTGTTGCTCTTTATACTGATTTATATATTTTATTCTACGAAAGACTTAAGAGTGTGTTTGGATGAACTTTTTAAGTGTTTATATATATATTTTTTTTGCACTTACAAACATTTTTGAATACTTAAAAAGTCTATCCAAACTAGCACTAAGTTTCTGTTTGGAATTGATTTTGGAGGCGTTAAGAGTGATTACATGAAGAATTTAACTGTTTTAGTGTTTGATTAGACTAACTATTAATTGATCCAAAGAAATTTACAATTATACGGATTCATAATGTAGCTTTCGGGATCAAATTAAGTTTTTAAGATTCTCATATTATTCCATTTTATTCTTCTTTTTCACTTGTAAAATTTTCCCGAATCCTTATCTACTCCGTATTACAATTAATTTTTTTAAAAGCATGTTTGACGGCCATTAGTGCTATACCGTAGTCTCAAATTAAGACTAAATTTTGTTTAAATATTCAGCAAAGCCTTTCCTTTATTGTTACCGTGGGCTTCTTCCGTTGATCTTGATGCAACAATCCTTTCTTTTGTCGCTTTGTTAAATCTGCAGGTGTTGAGACGACAAGGCATTTCTTATTGGAATGGCTTAGCTACACATGTAGATATATTCCCGTTGGTCTTCTGGAAGTTGTTCCCCAACGTCTAAACTGGCGACCACCATCCTATTTTGGCCGTGACGACCTTGAGACGCTCATGTCCTCTGACTCTGCAGCTGACTGGGTAACACTATAACTCATGCTTAATAGTAATACCATTTCATTGCAAGGTGAAAAATCAAACCATCGAACTTGAATTGTACCCTCTCAATTTTGCAGATTAAGATCTCAGAGATGTTGCTTGGTAAAGTTCCTGATGGCTTCTCGTTCGCTCCGAAACACAAGTCCAATGCCTATGATCGAGCCGAAAATGGCTAATACACAGAATTCATCACCGTAAGGATATTGGATCGCTGCAATGCTCTTCTTGGTGCCATTCCTGTGGGAGTATAGCAAGGCCTTGCTGCCTAACATCGCCAAGTTTAGTTCAGCGGTTGAAGAGTTAACTTAGATTTTTCCACAAATTATTTATATATTTATGTTCTGATTACGTCATAAATCTGATGCAGTAAGAAAAAAAAAAGATAAATCTGTTGGGCTCTCTTTCTGTTGATTATTAAATTATTTATTATTATTTTTATTTGATAAAACTAGGTTTCTAGTCATCATTTTTGTTACATTTATTTTGTCCTCAATGGATTAATGACCCAATTTGTAGCATGATTTGTAGCAAGGAAAACAGAAATGAGCATGTTTTCTAGTTGCATAGGTAGGAGACGAGAGTTTGGCAAAGAGAACCTTTTATAGAAAAATGGTATATGAAGACCTCAGTGGTCATTTTTCGAAATGTGTTTTAAATCTTTGAAATTTTATTGACTCTATTAGGTTTTTAAACTTAAAGAATAATTTATGAAAGTTTAATT

mRNA sequence

ATGGAAGAAGACTCGGTTACGGCGGACCTCCTCGACGACACCGCGGTCTCCGAGCACCCTCTTTCTCACTCATCCGACAACGGTACTCACTTGACGCCTCAACAACTAGTAGCCAAGGCCATAGCTCCCGTTAAGCGTGACTTTCTTCGCCCTCCGCCTCTCAGGTCATCCTCTAACAACCTTAAGGATGCTACTTCCACTGCTAAGAATGATGGTGATGATAAAGCTGCTCCCTCCACCTTGGTCAAGGAGAAGAAGTCCAAACGTCAATTGAAACGTGAACGCCGTCAGGAACAGAAGTCCACTCGTCACTTGTGTCCTGAAATAGCGAAGACAGGAGATGTTAGTTCATGTCGGTATAGTGATAAATGCCGATTTAGTCATGATATTGAAGCCTTTAAATCTCAGAAACCAGCTGATCTCGAAGGAGAGTGTCCATTCAGTGGTGCTGAACAACTTTGCCCTTACGGTTTAGCGTGTAGATTTGCGGGGACCCATAAGGAAGGTGTTCCTGCAGGTTCTTCAGTTACTTTGAAGAGATGCTCTGAAATGAATGGATTGAATAAAGATATTCAGAAGCTCTTATGGAAAAATAAGATGAAATTTCCCAAAGCTGATTCCAAACTCAAAGAACTTGGGCTCTTGGGGCATGGAAAGTCAAAATTAAGGGTCAAAGAAGATGAGGAAAATGCTGTATCGAATGACAGTGTGGTTGCGGACAGACCATGCTGCAGTGAAATGAACAATGACTTGGATGCTGACTTAGAGATCTCTGTGGAAGTGCCAGAAGAAAATGGGGAGAAAGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAATCAAAATCTTTGTCTTCAGAGAATTTTCATTCTGATCAAATTGACAATGGGGCATGTATGATTGATGAAACTATCGGTGAGGGCCATGCGCCAATCGAATCTGAAGCTATGAACGACAGTGTACTTCTTGATAGTGATGCAAGTCTAAAACCACATCCACGTGAAAAAAAGCTTATTGATTTTAGAGATAAGCTGTATCTCGCTCCTCTGACCACTGTCGGTAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGACGTGACATGCGGTGAAATGGCAATGTGTACAAATCTATTGCAGGGTCAAGCATCAGAATGGGCATTGTTGAGACGACATTCTTCAGAAGATCTATTTGGTGTACAGATATGTGGTGCATATCCAGACACTGTTTCGCGTGCTGTAGAACTTATAGACAAAGAATGCGTGCTTGACTTCATTGACATAAACATGGGATGCCCAATTGATATTGTCGTGAATAAGGGTGCTGGATCAGCTCTTCTTACAAAGCCAATGCGTATGAAAACTGTTGTAGAAGCAGCTTCGGGAATTGTGGATACACCCATAACAATTAAGGTTCGGACAGGTTACTATGAAGGGAAAAACCGTATCGATTCGCTTATTGCAGATATTGGTAACTGGGGAGCAAGTGCTGTAACCATTCATGGTCGGACTCGTCAGCAACGATACAGCAAGCTTGCTGACTGGGAATACATCAATCGGTGTTCCTCGTTGGCTCCTGATTCTTTGCAAGTTATAGGAAACGGGGATGTATTTTCATATACAGATTGGAATGAACACAAGAATGAATGCCCAAAGTTAGCCACATGCATGATCGCTAGAGGAGCACTAATTAAGCCTTGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATCACATCAGGGGAGCGGCTAAACATCTTGAAGGATTTTGCTCACTTTGGACTTGAGCATTGGGGATCCGATTCCAAAGGTGTTGAGACGACAAGGCATTTCTTATTGGAATGGCTTAGCTACACATGTAGATATATTCCCGTTGGTCTTCTGGAAGTTGTTCCCCAACGTCTAAACTGGCGACCACCATCCTATTTTGGCCGTGACGACCTTGAGACGCTCATGTCCTCTGACTCTGCAGCTGACTGGATTAAGATCTCAGAGATGTTGCTTGGTAAAGTTCCTGATGGCTTCTCGTTCGCTCCGAAACACAAGTCCAATGCCTATGATCGAGCCGAAAATGGCTAA

Coding sequence (CDS)

ATGGAAGAAGACTCGGTTACGGCGGACCTCCTCGACGACACCGCGGTCTCCGAGCACCCTCTTTCTCACTCATCCGACAACGGTACTCACTTGACGCCTCAACAACTAGTAGCCAAGGCCATAGCTCCCGTTAAGCGTGACTTTCTTCGCCCTCCGCCTCTCAGGTCATCCTCTAACAACCTTAAGGATGCTACTTCCACTGCTAAGAATGATGGTGATGATAAAGCTGCTCCCTCCACCTTGGTCAAGGAGAAGAAGTCCAAACGTCAATTGAAACGTGAACGCCGTCAGGAACAGAAGTCCACTCGTCACTTGTGTCCTGAAATAGCGAAGACAGGAGATGTTAGTTCATGTCGGTATAGTGATAAATGCCGATTTAGTCATGATATTGAAGCCTTTAAATCTCAGAAACCAGCTGATCTCGAAGGAGAGTGTCCATTCAGTGGTGCTGAACAACTTTGCCCTTACGGTTTAGCGTGTAGATTTGCGGGGACCCATAAGGAAGGTGTTCCTGCAGGTTCTTCAGTTACTTTGAAGAGATGCTCTGAAATGAATGGATTGAATAAAGATATTCAGAAGCTCTTATGGAAAAATAAGATGAAATTTCCCAAAGCTGATTCCAAACTCAAAGAACTTGGGCTCTTGGGGCATGGAAAGTCAAAATTAAGGGTCAAAGAAGATGAGGAAAATGCTGTATCGAATGACAGTGTGGTTGCGGACAGACCATGCTGCAGTGAAATGAACAATGACTTGGATGCTGACTTAGAGATCTCTGTGGAAGTGCCAGAAGAAAATGGGGAGAAAGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAATCAAAATCTTTGTCTTCAGAGAATTTTCATTCTGATCAAATTGACAATGGGGCATGTATGATTGATGAAACTATCGGTGAGGGCCATGCGCCAATCGAATCTGAAGCTATGAACGACAGTGTACTTCTTGATAGTGATGCAAGTCTAAAACCACATCCACGTGAAAAAAAGCTTATTGATTTTAGAGATAAGCTGTATCTCGCTCCTCTGACCACTGTCGGTAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGACGTGACATGCGGTGAAATGGCAATGTGTACAAATCTATTGCAGGGTCAAGCATCAGAATGGGCATTGTTGAGACGACATTCTTCAGAAGATCTATTTGGTGTACAGATATGTGGTGCATATCCAGACACTGTTTCGCGTGCTGTAGAACTTATAGACAAAGAATGCGTGCTTGACTTCATTGACATAAACATGGGATGCCCAATTGATATTGTCGTGAATAAGGGTGCTGGATCAGCTCTTCTTACAAAGCCAATGCGTATGAAAACTGTTGTAGAAGCAGCTTCGGGAATTGTGGATACACCCATAACAATTAAGGTTCGGACAGGTTACTATGAAGGGAAAAACCGTATCGATTCGCTTATTGCAGATATTGGTAACTGGGGAGCAAGTGCTGTAACCATTCATGGTCGGACTCGTCAGCAACGATACAGCAAGCTTGCTGACTGGGAATACATCAATCGGTGTTCCTCGTTGGCTCCTGATTCTTTGCAAGTTATAGGAAACGGGGATGTATTTTCATATACAGATTGGAATGAACACAAGAATGAATGCCCAAAGTTAGCCACATGCATGATCGCTAGAGGAGCACTAATTAAGCCTTGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATCACATCAGGGGAGCGGCTAAACATCTTGAAGGATTTTGCTCACTTTGGACTTGAGCATTGGGGATCCGATTCCAAAGGTGTTGAGACGACAAGGCATTTCTTATTGGAATGGCTTAGCTACACATGTAGATATATTCCCGTTGGTCTTCTGGAAGTTGTTCCCCAACGTCTAAACTGGCGACCACCATCCTATTTTGGCCGTGACGACCTTGAGACGCTCATGTCCTCTGACTCTGCAGCTGACTGGATTAAGATCTCAGAGATGTTGCTTGGTAAAGTTCCTGATGGCTTCTCGTTCGCTCCGAAACACAAGTCCAATGCCTATGATCGAGCCGAAAATGGCTAA

Protein sequence

MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLKDATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRPCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
BLAST of CsaV3_7G000530 vs. NCBI nr
Match: XP_004148548.1 (PREDICTED: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 hypothetical protein Csa_7G007900 [Cucumis sativus])

HSP 1 Score: 1303.1 bits (3371), Expect = 0.0e+00
Identity = 698/698 (100.00%), Postives = 698/698 (100.00%), Query Frame = 0

Query: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX 60
           MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX
Sbjct: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY 120
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240

Query: 241 RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA
Sbjct: 241 RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 600
           VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG
Sbjct: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of CsaV3_7G000530 vs. NCBI nr
Match: XP_008448025.1 (PREDICTED: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like isoform X1 [Cucumis melo])

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 633/698 (90.69%), Postives = 639/698 (91.55%), Query Frame = 0

Query: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX 60
           MEEDS+       TAVSEHPLSHS+DNGTH TPQQLVAKAIAPV  DFLRPPPLR     
Sbjct: 1   MEEDSL-------TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNT 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY 120
                                      XXXXXXXXXXXX STRHLCPEIAKTGDV+SCRY
Sbjct: 61  LKDATSTANNDGDDKAGPSTLVKEKKSXXXXXXXXXXXXKSTRHLCPEIAKTGDVNSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKED+ENAVSNDSVVAD
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAD 240

Query: 241 RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           R CCSE NNDL  XXXXXXXXXX       TFSSDESRPLKKSKSLSS NFHSDQIDNGA
Sbjct: 241 RTCCSETNNDLXXXXXXXXXXXXXXXXXXXTFSSDESRPLKKSKSLSSGNFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAPIESE MND VLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 600
           VFSY DWN+HK+ECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFG
Sbjct: 541 VFSYADWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of CsaV3_7G000530 vs. NCBI nr
Match: XP_022969416.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima])

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 613/696 (88.07%), Postives = 638/696 (91.67%), Query Frame = 0

Query: 3   EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXXXX 62
           EDSV+A+L D TAVSE P S S+  G  LTP++LVAKAIAPVKR++LRPPP+R       
Sbjct: 2   EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61

Query: 63  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRYSD 122
                      XXXXXXXXXXXXXXXXXXXXXXXXXXXST HLCPEIAKTGDVSSCRYSD
Sbjct: 62  DAASTGKNDGDXXXXXXXXXXXXXXXXXXXXXXXXXXXSTSHLCPEIAKTGDVSSCRYSD 121

Query: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
           KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CS
Sbjct: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCS 181

Query: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242
           EMNGLNKD+QKLLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE++E+AVSNDS VADR 
Sbjct: 182 EMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRT 241

Query: 243 CCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302
           CCSE+NN    XXXXXXXXXX       TFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 242 CCSEVNNXXXXXXXXXXXXXXXXXXXXXTFSSDESRPLKKPKSMSSENCHSDEIDNGACM 301

Query: 303 IDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362
           ID TI EGH PIESE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 302 IDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361

Query: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421

Query: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482
           KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFE 481

Query: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542
           GKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 482 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541

Query: 543 SYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLE 602
           SY +WN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGLE
Sbjct: 542 SYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 601

Query: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662
           HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661

Query: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 662 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696

BLAST of CsaV3_7G000530 vs. NCBI nr
Match: XP_023554076.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 606/696 (87.07%), Postives = 633/696 (90.95%), Query Frame = 0

Query: 3   EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXXXX 62
           EDSV+A+L   TAVSE P S S+  G  LTP++LVAKAIAPVKR++LRPPP+R       
Sbjct: 2   EDSVSAELRVPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSGNNVK 61

Query: 63  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRYSD 122
                           XXXXXXXXXXXXXXXXXXXXXXST HLCPEIAKTGDVSSCRYSD
Sbjct: 62  DAASTGKNDGDNKAAPXXXXXXXXXXXXXXXXXXXXXXSTSHLCPEIAKTGDVSSCRYSD 121

Query: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
           KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV+LK+CS
Sbjct: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVSLKKCS 181

Query: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242
           EMNGLNKD+QKLLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE++E+AVSNDS VADR 
Sbjct: 182 EMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRT 241

Query: 243 CCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302
           CCSE+NND   XXXXXXXXXX      GTFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 242 CCSEVNNDXXXXXXXXXXXXXXXXXXXGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 301

Query: 303 IDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362
           ID TI EGH PIESE +ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 302 IDATISEGHTPIESEVVNDIVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361

Query: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421

Query: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482
           KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 481

Query: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542
           GKNRIDSLIADIGNWGASAVT+HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 482 GKNRIDSLIADIGNWGASAVTVHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541

Query: 543 SYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLE 602
           SY DWN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILK+F HFGLE
Sbjct: 542 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGLE 601

Query: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662
           HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661

Query: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 662 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of CsaV3_7G000530 vs. NCBI nr
Match: XP_022952746.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 603/696 (86.64%), Postives = 629/696 (90.37%), Query Frame = 0

Query: 3   EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXXXX 62
           EDSV+A+L D TAVSE P S S+  G  LTP++LVAKAIAPVKR++LRPPP+R       
Sbjct: 2   EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNVK 61

Query: 63  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRYSD 122
                          XXXXXXXXXXXXXXXXXXXXXXXST HLCPEIAKTGDVSSCRYSD
Sbjct: 62  DTASTGKNDGDDKAAXXXXXXXXXXXXXXXXXXXXXXXSTSHLCPEIAKTGDVSSCRYSD 121

Query: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
           KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CS
Sbjct: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCS 181

Query: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242
           EMNGLNKD+QKLLWKNKMKFPKADSKLKELGLLGHGKSK RVKE++E+ VSNDS  ADR 
Sbjct: 182 EMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRT 241

Query: 243 CCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302
            C E+NND+  XXXXXXXXXX       TFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 242 RCGEVNNDMXXXXXXXXXXXXXXXXXXXTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 301

Query: 303 IDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362
           ID TI EGH PI+SE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 302 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361

Query: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421

Query: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482
           KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 481

Query: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542
           GKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 482 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541

Query: 543 SYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLE 602
           SY DWN HK E PKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+F HFGLE
Sbjct: 542 SYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGLE 601

Query: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662
           HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661

Query: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 662 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of CsaV3_7G000530 vs. TAIR10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein)

HSP 1 Score: 830.5 bits (2144), Expect = 7.8e-241
Identity = 416/683 (60.91%), Postives = 507/683 (74.23%), Query Frame = 0

Query: 32  TPQQLVAKAIAPVKRDFLRPPPLRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 91
           T ++L+ +++AP+K++FL PPP                                      
Sbjct: 36  TSEELIERSMAPIKKEFLCPPP----------PSRSVKQNDAADVRAPQSGLVQEKKSKR 95

Query: 92  XXXXXXXXXSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGAE 151
                    ST +LCP++A+T DV SC+Y DKCRF+HDIEAFK+QK  D+EG+CPF  + 
Sbjct: 96  QLKRERREQSTINLCPQVARTEDVDSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASG 155

Query: 152 QLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQKLLWKNKMKFPKADSKLKE 211
             C YGL+CRF G+H++    G+S   K  SEMN  NK+ Q+LLWKNKM F  AD+KLK 
Sbjct: 156 MKCAYGLSCRFLGSHRD--ITGNSDD-KEKSEMNFFNKETQRLLWKNKMTFTNADAKLKS 215

Query: 212 LGLLGHGKSKLRVKE-------DEENAVSNDSVVADRPCCSEMNNDLDAXXXXXXXXXXE 271
           LGLLGH K     +E       +  N      V  D    SE  +++            +
Sbjct: 216 LGLLGHAKKSNAAEEITAEKTQNGMNGTQATEVAVDSAVSSEHTSEM-----------IQ 275

Query: 272 NGEKEGTFSSDESRPLKKSKSLSSENFHSDQID---NGACMIDETIGEGH----APIESE 331
           + +  G   ++E RP+KK+KS   +N  +  +    +G  + +ET   G+    A +E E
Sbjct: 276 DVDIPGPLETEEVRPMKKAKSEDQKNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDE 335

Query: 332 AMNDSV-LLDSDASLKPHPRE-KKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE 391
              DS+ ++++D+SLK HPRE KKLIDFRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGE
Sbjct: 336 ---DSIKIVETDSSLKLHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGE 395

Query: 392 MAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMG 451
           MAMCTNLLQGQASEWALLRRHSSEDLFGVQICG+YPDTVSR VELID+EC +DFIDINMG
Sbjct: 396 MAMCTNLLQGQASEWALLRRHSSEDLFGVQICGSYPDTVSRVVELIDRECTVDFIDINMG 455

Query: 452 CPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIG 511
           CPID+VVNK AGSALL KP+RMK +VE +S IV+TPITIKVRT ++EGKNRIDSLIADIG
Sbjct: 456 CPIDMVVNKSAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFEGKNRIDSLIADIG 515

Query: 512 NWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECP 571
           NWGA+AVTIHGR+RQQRYSK ADW+YI +C+  A  +LQVIGNGDV+SY DWN+HK++CP
Sbjct: 516 NWGATAVTIHGRSRQQRYSKSADWDYIYQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCP 575

Query: 572 KLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTR 631
           +L++CMIARGALIKPW+FTEIKEQR+WDITSGERLNI+KDF  FGL+HWGSD+KGVETTR
Sbjct: 576 ELSSCMIARGALIKPWIFTEIKEQRHWDITSGERLNIMKDFVRFGLQHWGSDTKGVETTR 635

Query: 632 HFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLG 691
           HFLLEWLSYT RYIPVGLL+V+PQ++NWRPPSYFGRDDLETLM S+SA DW++ISEMLLG
Sbjct: 636 HFLLEWLSYTFRYIPVGLLDVIPQQINWRPPSYFGRDDLETLMMSESAGDWVRISEMLLG 691

Query: 692 KVPDGFSFAPKHKSNAYDRAENG 699
           KVP+GF+FAPKHKSNAYDRAENG
Sbjct: 696 KVPEGFTFAPKHKSNAYDRAENG 691

BLAST of CsaV3_7G000530 vs. TAIR10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein)

HSP 1 Score: 106.7 bits (265), Expect = 5.9e-23
Identity = 83/309 (26.86%), Postives = 142/309 (45.95%), Query Frame = 0

Query: 341 IDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 400
           +D+++KL LAP+  VG L FR +    GAD+T GE      +  C   L   +     + 
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 401 RHSSEDLFGV----------QICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNK 460
           + +   +F            Q+  +      +A E++  +     IDINMGCP    +  
Sbjct: 61  KGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVAT--IDINMGCPKAFSIQG 120

Query: 461 GAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASAVTI 520
           G G+ALL+KP  +  ++      +D P+T K+R    +       L   I   G  A+ +
Sbjct: 121 GMGAALLSKPELIHDILATLKRNLDVPVTCKIR--LLKSPADTVELARRIEKLGVPALAV 180

Query: 521 HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECPKLATCMIAR 580
           HGR    R    A W+ I     +A  S+ VI NGDV  Y D++  K      A+ M+AR
Sbjct: 181 HGRKIADRPRDPAKWDEI--ADVVAALSIPVIANGDVLEYDDFSRIKT-ATGAASVMVAR 240

Query: 581 GALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTRHFLLEWLS- 633
           GA+    +F+  K + +W+       ++ K +    +  W +D   V++T++ + E ++ 
Sbjct: 241 GAMWNASIFSP-KGKSHWE-------DVKKKYLRKSI-LWNND---VKSTKYTIKEMIAH 290

BLAST of CsaV3_7G000530 vs. TAIR10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein)

HSP 1 Score: 78.2 bits (191), Expect = 2.3e-14
Identity = 58/204 (28.43%), Postives = 96/204 (47.06%), Query Frame = 0

Query: 344 RDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM---AMCTNLLQGQASEWALLRRHSSED 403
           R K  +AP+     LPFR +C+  GA      M    + T   + +  E+   +      
Sbjct: 87  RPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQEFTTCK--EDRP 146

Query: 404 LFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTV 463
           LF VQ C   PDT+  A + ++  C  D++DIN+GCP  I      G+ L+     +K++
Sbjct: 147 LF-VQFCANDPDTLLEAAKRVEPYC--DYVDINLGCPQRIARRGNYGAFLMDNLPLVKSL 206

Query: 464 VEAASGIVDTPITIKVRT-GYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSK--LA 523
           VE  +  ++ P++ K+R     E   +   ++ D    G S + +HGRTR ++  K   A
Sbjct: 207 VEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDA---GCSLLAVHGRTRDEKDGKKFRA 266

Query: 524 DWEYINRCSSLAPDSLQVIGNGDV 542
           DW  I    +     + V+ NG+V
Sbjct: 267 DWSAIKEVKNAM--RIPVLANGNV 280

BLAST of CsaV3_7G000530 vs. Swiss-Prot
Match: sp|Q9T0J6|DUS3L_ARATH (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=3702 GN=At4g38890 PE=1 SV=2)

HSP 1 Score: 830.5 bits (2144), Expect = 1.4e-239
Identity = 416/683 (60.91%), Postives = 507/683 (74.23%), Query Frame = 0

Query: 32  TPQQLVAKAIAPVKRDFLRPPPLRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 91
           T ++L+ +++AP+K++FL PPP                                      
Sbjct: 36  TSEELIERSMAPIKKEFLCPPP----------PSRSVKQNDAADVRAPQSGLVQEKKSKR 95

Query: 92  XXXXXXXXXSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGAE 151
                    ST +LCP++A+T DV SC+Y DKCRF+HDIEAFK+QK  D+EG+CPF  + 
Sbjct: 96  QLKRERREQSTINLCPQVARTEDVDSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASG 155

Query: 152 QLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQKLLWKNKMKFPKADSKLKE 211
             C YGL+CRF G+H++    G+S   K  SEMN  NK+ Q+LLWKNKM F  AD+KLK 
Sbjct: 156 MKCAYGLSCRFLGSHRD--ITGNSDD-KEKSEMNFFNKETQRLLWKNKMTFTNADAKLKS 215

Query: 212 LGLLGHGKSKLRVKE-------DEENAVSNDSVVADRPCCSEMNNDLDAXXXXXXXXXXE 271
           LGLLGH K     +E       +  N      V  D    SE  +++            +
Sbjct: 216 LGLLGHAKKSNAAEEITAEKTQNGMNGTQATEVAVDSAVSSEHTSEM-----------IQ 275

Query: 272 NGEKEGTFSSDESRPLKKSKSLSSENFHSDQID---NGACMIDETIGEGH----APIESE 331
           + +  G   ++E RP+KK+KS   +N  +  +    +G  + +ET   G+    A +E E
Sbjct: 276 DVDIPGPLETEEVRPMKKAKSEDQKNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDE 335

Query: 332 AMNDSV-LLDSDASLKPHPRE-KKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE 391
              DS+ ++++D+SLK HPRE KKLIDFRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGE
Sbjct: 336 ---DSIKIVETDSSLKLHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGE 395

Query: 392 MAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMG 451
           MAMCTNLLQGQASEWALLRRHSSEDLFGVQICG+YPDTVSR VELID+EC +DFIDINMG
Sbjct: 396 MAMCTNLLQGQASEWALLRRHSSEDLFGVQICGSYPDTVSRVVELIDRECTVDFIDINMG 455

Query: 452 CPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIG 511
           CPID+VVNK AGSALL KP+RMK +VE +S IV+TPITIKVRT ++EGKNRIDSLIADIG
Sbjct: 456 CPIDMVVNKSAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFEGKNRIDSLIADIG 515

Query: 512 NWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECP 571
           NWGA+AVTIHGR+RQQRYSK ADW+YI +C+  A  +LQVIGNGDV+SY DWN+HK++CP
Sbjct: 516 NWGATAVTIHGRSRQQRYSKSADWDYIYQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCP 575

Query: 572 KLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTR 631
           +L++CMIARGALIKPW+FTEIKEQR+WDITSGERLNI+KDF  FGL+HWGSD+KGVETTR
Sbjct: 576 ELSSCMIARGALIKPWIFTEIKEQRHWDITSGERLNIMKDFVRFGLQHWGSDTKGVETTR 635

Query: 632 HFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLG 691
           HFLLEWLSYT RYIPVGLL+V+PQ++NWRPPSYFGRDDLETLM S+SA DW++ISEMLLG
Sbjct: 636 HFLLEWLSYTFRYIPVGLLDVIPQQINWRPPSYFGRDDLETLMMSESAGDWVRISEMLLG 691

Query: 692 KVPDGFSFAPKHKSNAYDRAENG 699
           KVP+GF+FAPKHKSNAYDRAENG
Sbjct: 696 KVPEGFTFAPKHKSNAYDRAENG 691

BLAST of CsaV3_7G000530 vs. Swiss-Prot
Match: sp|Q7XT07|DUS3L_ORYSJ (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0117600 PE=2 SV=2)

HSP 1 Score: 816.6 bits (2108), Expect = 2.1e-235
Identity = 396/603 (65.67%), Postives = 468/603 (77.61%), Query Frame = 0

Query: 104 HLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFA 163
           HLC E+ K+G+VSSC+Y D CRFSHDI+A+ +QKPADLEG CPF+  +QLCPYGL CRF 
Sbjct: 91  HLCIEVGKSGNVSSCKYGDSCRFSHDIDAYLAQKPADLEGTCPFTNLDQLCPYGLTCRFL 150

Query: 164 GTHKEGVPAGSSVTLKRCSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLR 223
           GTHK+   A  +++ K   E+N LNKDIQKLLWKNK KFPKA +++K LGL    KSK  
Sbjct: 151 GTHKDIHAASGNLSEKH--EINALNKDIQKLLWKNKYKFPKASAQIKLLGLKEVIKSKPD 210

Query: 224 VKEDE-----ENAVSNDSVVADRPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESR 283
              D+     +N   ND    +  C   +N + D+               + +    E R
Sbjct: 211 AANDDKKVNHDNLDGNDDENKEPLCNPPVNAECDSTLCEELDRSEGEPLIDNSIPCVEPR 270

Query: 284 PLKKSKSLSSENFHSDQIDNGACMIDETIGEGHAPIES---EAMNDSVLLDSDASLKPHP 343
           P KKSK        SD+ID        T  E   P  S   E  N+S    +D    PH 
Sbjct: 271 PTKKSK------VESDEIDKHGAGTLNTNTESEDPNLSNGLEPSNNSSSCRTDLITTPHL 330

Query: 344 REKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRR 403
           REKK+IDFR+KLYLAPLTTVGNLPFRR+CK LGAD+TCGEMAMCTNLLQGQASEWALLRR
Sbjct: 331 REKKIIDFREKLYLAPLTTVGNLPFRRLCKTLGADITCGEMAMCTNLLQGQASEWALLRR 390

Query: 404 HSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPM 463
           HSSEDLFGVQICGAYPDTV+R VEL+D EC +DFIDINMGCPIDIVVNKGAGS+LLTKPM
Sbjct: 391 HSSEDLFGVQICGAYPDTVARTVELVDNECSVDFIDINMGCPIDIVVNKGAGSSLLTKPM 450

Query: 464 RMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSK 523
           R+K++V+AAS + + P+T+KVRT ++EG+NR DS+++DI +WGASA+T+HGR+RQQRYSK
Sbjct: 451 RIKSIVQAASTVTEKPLTVKVRTAFFEGRNRADSIVSDIYDWGASAITVHGRSRQQRYSK 510

Query: 524 LADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECPKLATCMIARGALIKPWLFTE 583
           LADW+YI +C+  APD L V+GNGDVFS+TDWN+H + C K++T MIARGALIKPW+FTE
Sbjct: 511 LADWDYIYQCAQKAPDQLHVVGNGDVFSFTDWNKHVSGCSKISTSMIARGALIKPWIFTE 570

Query: 584 IKEQRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLE 643
           +KEQR+WDITSGER NILKDF  FGLEHWGSDSKGVETTR+FLLEWLSYTCRYIPVGLL+
Sbjct: 571 VKEQRHWDITSGERFNILKDFVSFGLEHWGSDSKGVETTRYFLLEWLSYTCRYIPVGLLD 630

Query: 644 VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRA 699
           V+PQRLNWRPPSY GRDDLETLM SDSAADWI+ISEMLLGKVP+GF+F PKHKSNAYDRA
Sbjct: 631 VIPQRLNWRPPSYCGRDDLETLMISDSAADWIRISEMLLGKVPEGFTFTPKHKSNAYDRA 685

BLAST of CsaV3_7G000530 vs. Swiss-Prot
Match: sp|Q91XI1|DUS3L_MOUSE (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus3l PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 6.9e-146
Identity = 266/594 (44.78%), Postives = 355/594 (59.76%), Query Frame = 0

Query: 105 LCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAG 164
           LCP   +    + C + D+CRF HD+  +   KPADL   C        CP+ + CRFAG
Sbjct: 112 LCPSFLQE-PATPCAFGDRCRFLHDVGRYLETKPADLGPRCVLFETFGRCPFSMTCRFAG 171

Query: 165 TH--KEGVPAGSSVTLKRCSEM----NGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHG 224
            H   EG        + RC+++    NGL++ +Q+ L K ++ F +A+  L  L      
Sbjct: 172 AHLGPEGQNLVQEEVVARCAQLPSVRNGLDRALQQQLRKRQVCFERAEQALNRL------ 231

Query: 225 KSKLRVKEDEENAVSNDSVVADRPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESR 284
                 +      V   +V    P  +  +  LD                          
Sbjct: 232 -----TQSPMPTVVPETTVAMATPKQNSCHAQLD-------------------------- 291

Query: 285 PLKKSKSLSSENFHSDQIDNGACMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREK 344
                                      T+G    P  S       L D D  ++  P EK
Sbjct: 292 ---------------------------TVGGAGTPQSSPVPTCGPLTDEDV-IRLRPCEK 351

Query: 345 KLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSS 404
           K +D   KLYLAPLTT GNLPFRR+CK  GADVTCGEMA+CTNLLQGQ SEWALL+RH  
Sbjct: 352 KRLDISGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHPC 411

Query: 405 EDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMK 464
           ED+FGVQ+ GA+PDT+++  EL+++   +DF+DIN+GCPID+V  KG G AL+ +  + +
Sbjct: 412 EDIFGVQLEGAFPDTMTKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQ 471

Query: 465 TVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLAD 524
            +V   +G++D P+T+K+RTG  E  +    L+ ++ +WG + VT+HGR+R+QRY++LAD
Sbjct: 472 QIVRGVNGVLDVPLTVKMRTGVQERVSLAHRLLPELRDWGVALVTLHGRSREQRYTRLAD 531

Query: 525 WEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKE 584
           W YI +C+ +A   + + GNGD+ S+ D N        +A  M+ARGAL+KPWLFTEIKE
Sbjct: 532 WPYIEQCAKVA-SPMPLFGNGDILSFEDANCAMQ--TGVAGIMVARGALLKPWLFTEIKE 591

Query: 585 QRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVP 644
           QR+WDI+S ERL+IL+DF H+GLEHWGSD++GVE TR FLLEWLS+ CRY+PVGLLE  P
Sbjct: 592 QRHWDISSSERLDILRDFTHYGLEHWGSDTQGVERTRRFLLEWLSFLCRYVPVGLLERPP 636

Query: 645 QRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 693
           QR+N RPP Y GRD LETLM+S  AADWI+ISEMLLG VP GF F PKHK+NAY
Sbjct: 652 QRINERPPYYLGRDYLETLMASQQAADWIRISEMLLGPVPPGFVFLPKHKANAY 636

BLAST of CsaV3_7G000530 vs. Swiss-Prot
Match: sp|Q3KRC5|DUS3L_RAT (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus3l PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.5e-145
Identity = 271/594 (45.62%), Postives = 362/594 (60.94%), Query Frame = 0

Query: 105 LCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAG 164
           LCP + +    + C + D+CRF HD+  +   KPADL   C        CPY + CRFAG
Sbjct: 115 LCPSLLQE-SATPCAFGDRCRFLHDVGRYLETKPADLGPHCVLFNTFGRCPYSMTCRFAG 174

Query: 165 TH--KEGVPAGSSVTLKRCSEM----NGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHG 224
            H   EG        + RC+++    NGL++ +Q+ L K ++ F +A+  L  L      
Sbjct: 175 AHLGPEGQNLVQEEVVARCAQLPSVRNGLDRALQQQLRKRQVCFERAEQALSHL------ 234

Query: 225 KSKLRVKEDEENAVSNDSVVADRPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESR 284
                  +     ++ +S VA                               T +   S 
Sbjct: 235 ------TQGPMPTIAPESTVA-------------------------------TLTPKHS- 294

Query: 285 PLKKSKSLSSENFHSDQIDNGACMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREK 344
                      + H  Q+DN        +G   A   S       L D D  ++  P EK
Sbjct: 295 -----------SCHV-QLDN--------VGGDGARQGSPVPTCGPLTDEDV-VRLRPCEK 354

Query: 345 KLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSS 404
           K +D   KLYLAPLTT GNLPFRR+CK  GADVTCGEMAMCTNLLQGQ SEWALL+RH  
Sbjct: 355 KRLDISGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAMCTNLLQGQMSEWALLKRHPC 414

Query: 405 EDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMK 464
           ED+FGVQ+ GA+PDT+++  EL+++   +DF+DIN+GCPID+V  KG G AL+ +  + +
Sbjct: 415 EDIFGVQLEGAFPDTMTKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQ 474

Query: 465 TVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLAD 524
            +V   + ++D P+T+K+RTG  E  +    L+ ++ NWG + VT+HGR+R+QRY++LAD
Sbjct: 475 QIVRGMNEVLDVPLTVKMRTGVQERVSLAHRLLPELRNWGVALVTLHGRSREQRYTRLAD 534

Query: 525 WEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKE 584
           W YI +C+ +A   + + GNGD+ S+ D N        +A  M+ARGAL+KPWLFTEIKE
Sbjct: 535 WPYIEQCAKVA-SPMPLFGNGDILSFEDANCAMQ--TGVAGIMVARGALLKPWLFTEIKE 594

Query: 585 QRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVP 644
           QR+WDI+S ERL+IL+DF H+GLEHWGSD++GVE TR FLLEWLS+ CRY+PVGLLE +P
Sbjct: 595 QRHWDISSSERLDILRDFTHYGLEHWGSDTQGVERTRRFLLEWLSFLCRYVPVGLLERLP 639

Query: 645 QRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 693
           QR+N RPP Y GRD LETLM+S  AADWI+ISEMLLG VP GF F PKHK+NAY
Sbjct: 655 QRINERPPYYLGRDYLETLMASQQAADWIRISEMLLGPVPPGFVFLPKHKANAY 639

BLAST of CsaV3_7G000530 vs. Swiss-Prot
Match: sp|Q96G46|DUS3L_HUMAN (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS3L PE=1 SV=2)

HSP 1 Score: 516.9 bits (1330), Expect = 3.4e-145
Identity = 270/596 (45.30%), Postives = 355/596 (59.56%), Query Frame = 0

Query: 105 LCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAG 164
           LCP + +    + C + D+CRF HD+  +   KPADL   C        CPYG+ CRFAG
Sbjct: 123 LCPSLIQE-SAAKCFFGDRCRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFAG 182

Query: 165 THKEGVPAGSSVTLKRC--------SEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLG 224
            H    P G ++  +          S  NGL+K +Q+ L K +++F +A+  L+      
Sbjct: 183 AHLR--PEGQNLVQEELAARGTQPPSIRNGLDKALQQQLRKREVRFERAEQALRRFS--- 242

Query: 225 HGKSKLRVKEDEENAVSNDSVVADRPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDE 284
                              +  A  P                          EGT +   
Sbjct: 243 ----------------QGPTPAAAVP--------------------------EGTAAEGA 302

Query: 285 SRPLKKSKSLSSENFHSDQIDNGACMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPR 344
            R          EN  + Q+  G             P  S       L D D  ++  P 
Sbjct: 303 PR---------QENCGAQQVPAGP--------GTSTPPSSPVRTCGPLTDEDV-VRLRPC 362

Query: 345 EKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRH 404
           EKK +D R KLYLAPLTT GNLPFRR+CK  GADVTCGEMA+CTNLLQGQ SEWALL+RH
Sbjct: 363 EKKRLDIRGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRH 422

Query: 405 SSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMR 464
             ED+FGVQ+ GA+PDT+++  EL+ +   +DF+DIN+GCPID+V  KG G AL+ +  +
Sbjct: 423 QCEDIFGVQLEGAFPDTMTKCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTK 482

Query: 465 MKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKL 524
            + +V   + ++D P+T+K+RTG  E  N    L+ ++ +WG + VT+HGR+R+QRY+KL
Sbjct: 483 FQQIVRGMNQVLDVPLTVKIRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKL 542

Query: 525 ADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEI 584
           ADW+YI  C   A   + + GNGD+ S+ D N        +   MIARGAL+KPWLFTEI
Sbjct: 543 ADWQYIEECVQ-AASPMPLFGNGDILSFEDANRAMQ--TGVTGIMIARGALLKPWLFTEI 602

Query: 585 KEQRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEV 644
           KEQR+WDI+S ERL+IL+DF ++GLEHWGSD++GVE TR FLLEWLS+ CRY+PVGLLE 
Sbjct: 603 KEQRHWDISSSERLDILRDFTNYGLEHWGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLER 649

Query: 645 VPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 693
           +PQR+N RPP Y GRD LETLM+S  AADWI+ISEMLLG VP  F+F PKHK+NAY
Sbjct: 663 LPQRINERPPYYLGRDYLETLMASQKAADWIRISEMLLGPVPPSFAFLPKHKANAY 649

BLAST of CsaV3_7G000530 vs. TrEMBL
Match: tr|A0A0A0K4I1|A0A0A0K4I1_CUCSA (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7G007900 PE=3 SV=1)

HSP 1 Score: 1303.1 bits (3371), Expect = 0.0e+00
Identity = 698/698 (100.00%), Postives = 698/698 (100.00%), Query Frame = 0

Query: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX 60
           MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX
Sbjct: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY 120
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240

Query: 241 RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA
Sbjct: 241 RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 600
           VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG
Sbjct: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of CsaV3_7G000530 vs. TrEMBL
Match: tr|A0A1S3BIS3|A0A1S3BIS3_CUCME (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC103490332 PE=3 SV=1)

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 633/698 (90.69%), Postives = 639/698 (91.55%), Query Frame = 0

Query: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX 60
           MEEDS+       TAVSEHPLSHS+DNGTH TPQQLVAKAIAPV  DFLRPPPLR     
Sbjct: 1   MEEDSL-------TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNT 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY 120
                                      XXXXXXXXXXXX STRHLCPEIAKTGDV+SCRY
Sbjct: 61  LKDATSTANNDGDDKAGPSTLVKEKKSXXXXXXXXXXXXKSTRHLCPEIAKTGDVNSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKED+ENAVSNDSVVAD
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAD 240

Query: 241 RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           R CCSE NNDL  XXXXXXXXXX       TFSSDESRPLKKSKSLSS NFHSDQIDNGA
Sbjct: 241 RTCCSETNNDLXXXXXXXXXXXXXXXXXXXTFSSDESRPLKKSKSLSSGNFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAPIESE MND VLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 600
           VFSY DWN+HK+ECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFG
Sbjct: 541 VFSYADWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of CsaV3_7G000530 vs. TrEMBL
Match: tr|A0A1S3BIR4|A0A1S3BIR4_CUCME (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC103490332 PE=3 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 9.0e-276
Identity = 506/571 (88.62%), Postives = 512/571 (89.67%), Query Frame = 0

Query: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXX 60
           MEEDS+       TAVSEHPLSHS+DNGTH TPQQLVAKAIAPV  DFLRPPPLR     
Sbjct: 1   MEEDSL-------TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNT 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRY 120
                                      XXXXXXXXXXXX STRHLCPEIAKTGDV+SCRY
Sbjct: 61  LKDATSTANNDGDDKAGPSTLVKEKKSXXXXXXXXXXXXKSTRHLCPEIAKTGDVNSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKED+ENAVSNDSVVAD
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAD 240

Query: 241 RPCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           R CCSE NNDL  XXXXXXXXXX       TFSSDESRPLKKSKSLSS NFHSDQIDNGA
Sbjct: 241 RTCCSETNNDLXXXXXXXXXXXXXXXXXXXTFSSDESRPLKKSKSLSSGNFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAPIESE MND VLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPW 572
           VFSY DWN+HK+ECPKLATCMIARGALIK W
Sbjct: 541 VFSYADWNQHKDECPKLATCMIARGALIKMW 564

BLAST of CsaV3_7G000530 vs. TrEMBL
Match: tr|A0A2I4E7G1|A0A2I4E7G1_9ROSI (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Juglans regia OX=51240 GN=LOC108986972 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 2.0e-267
Identity = 485/698 (69.48%), Postives = 555/698 (79.51%), Query Frame = 0

Query: 4   DSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRXXXXXXXX 63
           D   A+ + D  +S         NG+  TP++LVAKAIAPVK++FLR PP R        
Sbjct: 2   DESPAETVVDPKLSGDDSPSLKSNGSQRTPEELVAKAIAPVKKEFLRSPPPR-------P 61

Query: 64  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTRHLCPEIAKTGDVSSCRYSDK 123
                            XXXXXXXXXXXXXXXXXXXXS R +CP IAKTGDVSSC Y+DK
Sbjct: 62  SSSTNHQNHVVSETKVVXXXXXXXXXXXXXXXXXXXXSARSICPLIAKTGDVSSCPYNDK 121

Query: 124 CRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSE 183
           CRFSHD++AFK QKPADLEGECPF  AE  CPYGLACRF GTHK+GVP G+S   K+C+E
Sbjct: 122 CRFSHDLQAFKDQKPADLEGECPFMNAEGPCPYGLACRFFGTHKDGVPTGTSDARKKCAE 181

Query: 184 MNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDE--ENAVSNDSVVADR 243
           +NGL KD+QKLLWKNKM FPKAD+KLK LGLLG  KSK+++ E E  +  + +     + 
Sbjct: 182 INGLKKDVQKLLWKNKMSFPKADAKLKALGLLGKAKSKMKILEVEKDDETIPSTCHETNE 241

Query: 244 PCCSEMNNDLDAXXXXXXXXXXENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGAC 303
             CSE+NN+ DA          E+G  +GT  +DE +P KK KS+  ENF S +  NG  
Sbjct: 242 NGCSEVNNESDA-KLDFVDVPGEDG-VDGTLETDE-QPPKKQKSVICENFSSAEAKNGDV 301

Query: 304 MIDE-TIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 363
           +  E  +G      + E   D +  ++D SLK HPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 302 IFTEKNVGISCTQTKPEDPTDIIPPEADRSLKLHPREKKLIDFRDKLYLAPLTTVGNLPF 361

Query: 364 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 423
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV+RAVEL
Sbjct: 362 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVARAVEL 421

Query: 424 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 483
           ID+EC +DFIDINMGCPIDIVV+KGAGSALLTKPMR+K++++AASG VD PIT+KVRTG+
Sbjct: 422 IDQECTVDFIDINMGCPIDIVVDKGAGSALLTKPMRIKSIIDAASGTVDKPITVKVRTGF 481

Query: 484 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 543
           +EGKNRIDSLI DIG WG SAVTIHGR+RQQRYSKLA+W+YI +C+  APD LQV+GNGD
Sbjct: 482 FEGKNRIDSLIKDIGYWGTSAVTIHGRSRQQRYSKLANWDYIYQCARKAPDDLQVLGNGD 541

Query: 544 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 603
           VFSY DWN HK ECP LATCMIARGALIKPW+FTEIKEQ++WDI+SGERL ILKDF  FG
Sbjct: 542 VFSYLDWNMHKTECPVLATCMIARGALIKPWIFTEIKEQKHWDISSGERLTILKDFVRFG 601

Query: 604 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 663
           L+HWGSD+KGVETTR FLLEWLSYTCRYIPVGLL+VVPQ+LNWRPP+Y GRDDLETLM+S
Sbjct: 602 LDHWGSDTKGVETTRRFLLEWLSYTCRYIPVGLLDVVPQQLNWRPPTYMGRDDLETLMAS 661

Query: 664 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWI+ISEMLLGKVPDGF+FAPKHKSNAYDRAENG
Sbjct: 662 DSAADWIRISEMLLGKVPDGFTFAPKHKSNAYDRAENG 689

BLAST of CsaV3_7G000530 vs. TrEMBL
Match: tr|A0A2R6RBS0|A0A2R6RBS0_ACTCH (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc07287 PE=3 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 2.6e-267
Identity = 454/670 (67.76%), Postives = 526/670 (78.51%), Query Frame = 0

Query: 32  TPQQLVAKAIAPVKRDFLRPPPLRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 91
           TP+ LVAKAIAPVKR+FL PPP+R                                    
Sbjct: 37  TPEDLVAKAIAPVKREFLLPPPIR-----PCSKDSNDVASDATKSAPSNLLKEKKSKRQL 96

Query: 92  XXXXXXXXXSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGAE 151
                    S  HLCPE+A++GD S+CRY+DKCRFSHDIEAFK QKPADLEG+CPF   E
Sbjct: 97  KRERLQEKKSVLHLCPEVARSGDPSTCRYNDKCRFSHDIEAFKDQKPADLEGDCPFLNNE 156

Query: 152 QLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQKLLWKNKMKFPKADSKLKE 211
             CPYGL CRF+GTHK+G+PA +    K+  EMNGLNKD+QKLLWKN MKFPKAD++LK 
Sbjct: 157 GPCPYGLTCRFSGTHKDGLPAEAFNGQKKSPEMNGLNKDVQKLLWKNNMKFPKADAQLKL 216

Query: 212 LGLLGHGKS---KLRVKEDEENAVSNDSVVADRPCCSEMNNDLDAXXXXXXXXXXENGEK 271
           LGLLGH KS   KL  +E+ +    N S+ AD     E+  D +           E+   
Sbjct: 217 LGLLGHMKSVQAKLSEEEEGDRTTPNRSIAADENGGCEVAGDTNIKMDHSPQPPEEDDCA 276

Query: 272 EGTFSSDESRPLKKSKSLSSENFHSDQIDNGACMIDETIGEGHAPIESEAMNDSVLLDSD 331
            GTF+SDE RP KK+KS   EN   +++DNG   + + I +  +  E+ +  D +  ++D
Sbjct: 277 GGTFASDELRPPKKAKSSVDENHSRNELDNGIGKLKKDIEKSCSQNETGSTADCIPAETD 336

Query: 332 ASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQAS 391
            SLK HPREKKLIDFR+KLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQAS
Sbjct: 337 GSLKSHPREKKLIDFREKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQAS 396

Query: 392 EWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGS 451
           EWALLRRHSSEDLFGVQICGAYPDTV+R VELID+EC +DFIDINMGCPIDIVVNKGAGS
Sbjct: 397 EWALLRRHSSEDLFGVQICGAYPDTVTRTVELIDQECTVDFIDINMGCPIDIVVNKGAGS 456

Query: 452 ALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASAVTIHGRT 511
           ALL KPMRMK+VV+AAS IVD PIT+KVRTGY+EG+NRIDSLI +IG WGASAVTIHGR+
Sbjct: 457 ALLMKPMRMKSVVQAASAIVDRPITVKVRTGYFEGRNRIDSLIENIGQWGASAVTIHGRS 516

Query: 512 RQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNEHKNECPKLATCMIARGALI 571
           RQQRYSKLADWEYI +C+  APD+LQV+GNGDVFSY DWN HK++CP+L+TCMIARGALI
Sbjct: 517 RQQRYSKLADWEYIYQCTRKAPDALQVLGNGDVFSYLDWNTHKSDCPELSTCMIARGALI 576

Query: 572 KPWLFTEIKEQRNWDITSGERLNILKDFAHFGLEHWGSDSKGVETTRHFLLEWLSYTCRY 631
           KPW+FTEIKEQR+WDI+SGERLNI+KD+  FGL+HWGSD+KGVETTRHFLLEWLSYT RY
Sbjct: 577 KPWIFTEIKEQRHWDISSGERLNIMKDYVRFGLDHWGSDAKGVETTRHFLLEWLSYTFRY 636

Query: 632 IPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHK 691
           IPVGLL+V+PQRLNWRPPSYFGRDDLETLM+S+SA+DW++ISEMLLGKVP GF+FAPKHK
Sbjct: 637 IPVGLLDVIPQRLNWRPPSYFGRDDLETLMASESASDWVRISEMLLGKVPAGFTFAPKHK 696

Query: 692 SNAYDRAENG 699
           SNAYDRAENG
Sbjct: 697 SNAYDRAENG 701

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004148548.10.0e+00100.00PREDICTED: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >... [more]
XP_008448025.10.0e+0090.69PREDICTED: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like isoform X1 [Cucumis... [more]
XP_022969416.10.0e+0088.07tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima][more]
XP_023554076.10.0e+0087.07tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo][more]
XP_022952746.10.0e+0086.64tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G38890.17.8e-24160.91FMN-linked oxidoreductases superfamily protein[more]
AT3G49640.15.9e-2326.86Aldolase-type TIM barrel family protein[more]
AT5G67220.12.3e-1428.43FMN-linked oxidoreductases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9T0J6|DUS3L_ARATH1.4e-23960.91tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=370... [more]
sp|Q7XT07|DUS3L_ORYSJ2.1e-23565.67tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonic... [more]
sp|Q91XI1|DUS3L_MOUSE6.9e-14644.78tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Du... [more]
sp|Q3KRC5|DUS3L_RAT1.5e-14545.62tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 ... [more]
sp|Q96G46|DUS3L_HUMAN3.4e-14545.30tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0K4I1|A0A0A0K4I1_CUCSA0.0e+00100.00tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7... [more]
tr|A0A1S3BIS3|A0A1S3BIS3_CUCME0.0e+0090.69tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC10349... [more]
tr|A0A1S3BIR4|A0A1S3BIR4_CUCME9.0e-27688.62tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC10349... [more]
tr|A0A2I4E7G1|A0A2I4E7G1_9ROSI2.0e-26769.48tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Juglans regia OX=51240 GN=LOC108... [more]
tr|A0A2R6RBS0|A0A2R6RBS0_ACTCH2.6e-26767.76tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Actinidia chinensis var. chinens... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0046872metal ion binding
GO:0017150tRNA dihydrouridine synthase activity
GO:0050660flavin adenine dinucleotide binding
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008033tRNA processing
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR035587DUS-like_FMN-bd
IPR000571Znf_CCCH
IPR018517tRNA_hU_synthase_CS
IPR001269tRNA_hU_synthase
IPR013785Aldolase_TIM
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008033 tRNA processing
biological_process GO:0002943 tRNA dihydrouridine synthesis
cellular_component GO:0005575 cellular_component
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_7G000530.1CsaV3_7G000530.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 81..101
NoneNo IPR availableGENE3DG3DSA:4.10.1000.10coord: 95..174
e-value: 7.3E-6
score: 27.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..292
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..288
NoneNo IPR availablePANTHERPTHR11082:SF8TRNA-DIHYDROURIDINE 47 SYNTHASE NAD P + -LIKEcoord: 275..693
NoneNo IPR availableSUPERFAMILYSSF51395FMN-linked oxidoreductasescoord: 346..659
IPR013785Aldolase-type TIM barrelGENE3DG3DSA:3.20.20.70coord: 341..584
e-value: 7.8E-77
score: 259.6
IPR001269tRNA-dihydrouridine synthasePFAMPF01207Duscoord: 349..598
e-value: 1.2E-49
score: 169.0
IPR001269tRNA-dihydrouridine synthasePANTHERPTHR11082TRNA-DIHYDROURIDINE SYNTHASEcoord: 275..693
IPR018517tRNA-dihydrouridine synthase, conserved sitePROSITEPS01136UPF0034coord: 430..448
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 100..131
score: 10.85
IPR035587DUS-like, FMN-binding domainCDDcd02801DUS_like_FMNcoord: 346..580
e-value: 1.42427E-91
score: 285.928

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsaV3_7G000530Cucumber (Gy14) v2cgybcucB093
CsaV3_7G000530Cucumber (Gy14) v2cgybcucB095
CsaV3_7G000530Cucumber (Gy14) v1cgycucB257
CsaV3_7G000530Cucurbita maxima (Rimu)cmacucB0177
CsaV3_7G000530Cucurbita maxima (Rimu)cmacucB0231
CsaV3_7G000530Cucurbita maxima (Rimu)cmacucB0769
CsaV3_7G000530Cucurbita maxima (Rimu)cmacucB0958
CsaV3_7G000530Cucurbita moschata (Rifu)cmocucB0219
CsaV3_7G000530Cucurbita moschata (Rifu)cmocucB0757
CsaV3_7G000530Cucurbita moschata (Rifu)cmocucB0948
CsaV3_7G000530Cucurbita pepo (Zucchini)cpecucB0139
CsaV3_7G000530Cucurbita pepo (Zucchini)cpecucB0869
CsaV3_7G000530Cucurbita pepo (Zucchini)cpecucB0947
CsaV3_7G000530Cucurbita pepo (Zucchini)cpecucB1042
CsaV3_7G000530Wild cucumber (PI 183967)cpicucB118
CsaV3_7G000530Wild cucumber (PI 183967)cpicucB119
CsaV3_7G000530Bottle gourd (USVL1VR-Ls)cuclsiB524
CsaV3_7G000530Bottle gourd (USVL1VR-Ls)cuclsiB570
CsaV3_7G000530Melon (DHL92) v3.5.1cucmeB568
CsaV3_7G000530Melon (DHL92) v3.6.1cucmedB554
CsaV3_7G000530Watermelon (Charleston Gray)cucwcgB558
CsaV3_7G000530Watermelon (Charleston Gray)cucwcgB599
CsaV3_7G000530Watermelon (97103) v1cucwmB604
CsaV3_7G000530Watermelon (97103) v1cucwmB640
CsaV3_7G000530Watermelon (97103) v2cucwmbB539
CsaV3_7G000530Watermelon (97103) v2cucwmbB578
CsaV3_7G000530Wax gourdcucwgoB701
CsaV3_7G000530Wax gourdcucwgoB706
CsaV3_7G000530Cucumber (Chinese Long) v3cuccucB098
CsaV3_7G000530Cucumber (Chinese Long) v3cuccucB101
CsaV3_7G000530Silver-seed gourdcarcucB0244
CsaV3_7G000530Silver-seed gourdcarcucB1026