CsaV3_5G039430 (gene) Cucumber (Chinese Long) v3

NameCsaV3_5G039430
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
Descriptionprotein NRT1/ PTR FAMILY 4.4
Locationchr5 : 31187932 .. 31192151 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTATTATTTTGTAGTGCATATGAAGATTATTCAAATTAAAGGAAAATAAAGAGAGAGCCAAAAAATTCCCAAATGCAAAGAGATCATGAAGATATTTGTGTGGATTGGAGAGGGCGAGCTTGTAATCCTAACAGACATGGTGGAATGGGAGCTGCTGCCTTTGTCCTTGGTCAGCTATATATTCTGTTCTTTTATTTCTCTTGCTCATTTGATGGTCATTTCGTTTCTAAATTTAAGCTTGTTTTGTCTAATTTTTTGTCGAAATTTTGGTCAAATTCTGTGAAAGAGAGCTTGGTTAGTGATATTTGAAAAGAGATGTTAATGAAAACACAGAAAAATTGGTGTTTGTGTTTAACAATAATATTGTTTTGTTGATTAGGACTTCAAGCATTTGAGATGATGGCAATAGCAGCAGTTGGGAATAATCTGATAACATATGTTTTCAGTGAAATGCATTTTCCACTGTCTAAATCAGCAAACGTTGTTACCAACTTTGTTGGAACTGTCTTCTTTTTGTCTCTTCTTGGTGGCTTCTTGTCCGATTCTTATCTTGGTAGCTTTCGAACTATGCTCATCTTTGGCTTCATCGAACTCGCTGTATGTTTTACTAATTTCTTCTCTTCTCTCACCATCCATCATCACCTTTTTTCTTCTTCCCATTTTGTCCATTTCAATTACTTTTTCTCATGTGCTTAATTAATTCCAACTCCATTAATTGGATACACCATACCATGAAAAGAAAGATACTAATTTGGATGTATCTAGCTATCTGGGTTGCACTCCATCTAACATAATGGAAACTTTAAACAAAAAGAGGGTGCAGTTTAAAATCACTCAAGTATTGCTCTTTTGAAACACTATCAGTTATACTGTTTGAACTTGTTTATATTGGATCATACTTGAGAATACTTTGACCCTTTGTTTACTCTCTTTTTCTTCTTCTTTTTTGACTTCAACAATAAATCTCGACAAAGGGATTCTACGTTTTTATATCAAAAGTTGATCGAAAAGGTCTTTATTTTCTCTCTTTCCCTAATAAAGAAAAGCGATACTCAGTGTGTATTCTAGCCAAATACATCCAAAGAAACTTACAGATATTTTTTAGAAAATCAAATAAAAAAAAGAAGAGTTTGTCATGTAACAAACGAACTTGATATAATAAAAAAATATGGTACAAAGTATTTTTCTTTAAAATTGAGAACATAGTCTTTATTGTTGATGACCAAGTAATTAAATATTATTAAAAAAAGAAAAAGGAAAATGATCATATAGGTTAGTATAAGTAAAGCTTTATCATAATATATAGTTTGCCCTATGTGGCCTATACAACACAAATTAGTGGGAGGAAAATCATACATCTTAATGACTTTTTAACATAGGTGTGTGTATTATTATATAAATGTACAATATGGTGGGGTGGATTAGGTAGAGTAAGATGACAAAATATTTGGGTTTATCCCCTCTCTCAATCCCACATAATTAATGTCTAATCATTACTAAGCACGTACCAAAAATTCCTATTAAAATTTATTTTCAAAATAAAAGGTTATGATAGACTGAATTTTCAAAATGAATGATTATAATAGACGGATGAATAGGTGCACCCAAATATTTTTCTTTAATAAGATTAATAGATGTAAGTTTAATATTTTTCTTTAATAAGATTAATAGATGTAAGTTTGAAAGAAAAATATTTGAGTGCATTCTGGGTTGCACTTCACTCCATCGTCAACACATGACAACTTTTTTCTTTTGTAAAAAATATATTTTAGTACATCTTGGATTGCATTCATCTTTATATAATTCTTTTGCTTTATTATTTAACATGTTGTATGAAGATAGGTGCAAAGGGCTAGATTGCACCCAAGTATTATTAGTCGGTAATTTGAAACGGTGGATGATTAATTGGTGCAGGGATTTACAGTTCTAACGATACAAGCTCATTACCCAGGACTAAGGCCATCACCATGTGAGATGGGAAGAAAATTAGATGAAGAAGATGATCAATGCATAGAAGCCAAAGGATATGAAGCATTAATGTTCTTTTCAGCATTATATTTGGTAGCATTGGGAAGTGGATGTTTAAAGCCAAACATAATATCACATGGGGCAGACCAATTTAGAAAAGAAGACCCAAAACAATTCAAAAAGCTATCAACTTTCTTCAATTGTGCTTATTTTGCATTTTGCACTGGTGAACTCATTGCATTGACTCTTCTTGTTTGGGTTCAAACACATTCTGGCATGGACCTTGGCTTTGGTGTTTCCACTGCTGCTATGCTTCTTGCTTTGATTTCATTGCTCTCTGGCACTTCTTTTTATAGGAATAATCCCCCGCGTGGCAGTATCTTCACTCCAATTGCTCAAGTAAGTTTGGATACTCGGTTGTCAATCTCTCAGTCTTCTACCTCACAGACAAATGTCAGCCCTTCTTGACCTTTCTTATATCAAGTTAAATCCATTTTTTAAACAAACACGAATTTGCTCTTCGATAAAGTTACATTTTTGGTCCATGAGGTTTAGGTAAAGATTACATTTTGGTTCTAGATCAATTTTCAAATTTAAAAACTTTGTTGATAAAGTTTACATTTTGTTTGATTTTGATCTTTTGTTAACGACTTCCTTTTTTTTTTTAGGGTTTAAGGTAATTAAACATTAAACAAAACGAAATTGTCACAAATGAAGATAATGAGCGACAAGAATGTTTGTAAGCTTAATTTTAAACAGGAAAATTGTTTTAACTGAAAATATTTACGGATATAACGAAATTTTATAATCTATCAGTAATAAACAACGATATAGATAGACATATGTAATCATCGGTATCGTCTATTAACATCTATTAGTGATAGACTATGGTAGTATTTAAATGTCCATTAAACATGATCTATTAGTGATATTTTATCATACTCGTAAACATTCTGCTACTTTTTTCTTATATATTTGAAAGTGACTTTTTTTGTTTGTTGGATAGGTCTTGGTTGCTGCATATAGGAAAAGGAACCTACAAATGTGTCCTTCGAATTCAGAGATGGTACTCAATGGAACTCAAAATGGCATTGTCAAACTCATACACACCGATAAGTTCAGGTTTGTCATGTCCTTTCGGATCCCATCTCAATTATCAAACCTATGTTTTTCTTTTTCTTTATATATTTGTTGATTTCTTAACCATATTCATCCTTATTTACAGATTCCTTGACAAGGCTTGCATAAAAAGTGAAGAAGAAAGATTGGGATCAATTAGAGAAGAAAGTCCATGGAAGCTTTGCACAGTAAAACAAGTAGAACAAGTGAAAATAATACTCTCAGTAATTCCCATATTTGCTTGCACCATAATCTTCAACACCATTTTAGCACAACTCCAAACATTCTCAGTCCAACAAGGAGCTTCCATGAACACATGGCTCACAAATTCCTTCCAAATCCCCCCAGCTTCCCTCCAAGCCATCCCTTACATTATCCTCATCTTCCTCGTCCCTCTTTACGAGACCGTCTTTGTCCCACTCACTCGTCGCCTCACCTCCATCGACTCCGGCATCTCCCCTCTCCAACGTGTCGGCACCGGCCTCTTTGTCGCCACCTTCTCCATGGTCTCTGCTGCACTGGTCGAGCAAAAACGTCGAAACTCTTCCTCCTCCCTCTCCATCCTCTGGATTGCACCACAATTCGTGATATTCGGCGTGTCAGAGCTGTTTACGGCGGTCGGGTTGATCGAGTTTTTCTATAAGCAATCTGTGGAAGGGATGCAGTCATTTTTAACTGCCATGACTTATTGTTCATACTCCTTCGGGTTCTACTTGAGCTCTTTGTTGGTGTCACTTGTGAATAAGATCAGTGGTGGATGGCTTAGCCATAATGACCTAAATAATGATAGGTTGGATCTGTTCTATTGGTTGTTGGCTGGTCTTAGCTTTGTGAATTTCTTCAACTATCTCTTTTGGGCCAATAGATTTTCTCAACAACCTCCTTTGCCACTTCATCTTCTTCAAACCCAAACCTCAACCAAACAACCTTCAAACTCTAAGCTTGTAGTGCCTTGAAAGTTGAAACATATAACTACTTTTTTGTTGTGCTTGCTGATCTCTATTTTTAGTGGCTACTACAGATATATTTTAGTTGAAAACTACTCTACTTATGATCTTAGCTATTGAAAGCTAGTAATAATAATACAAAAAAATTATTATATATAAATATATGTATACCTTCTTAGTTATCA

mRNA sequence

ATGCAAAGAGATCATGAAGATATTTGTGTGGATTGGAGAGGGCGAGCTTGTAATCCTAACAGACATGGTGGAATGGGAGCTGCTGCCTTTGTCCTTGGACTTCAAGCATTTGAGATGATGGCAATAGCAGCAGTTGGGAATAATCTGATAACATATGTTTTCAGTGAAATGCATTTTCCACTGTCTAAATCAGCAAACGTTGTTACCAACTTTGTTGGAACTGTCTTCTTTTTGTCTCTTCTTGGTGGCTTCTTGTCCGATTCTTATCTTGGTAGCTTTCGAACTATGCTCATCTTTGGCTTCATCGAACTCGCTGGATTTACAGTTCTAACGATACAAGCTCATTACCCAGGACTAAGGCCATCACCATGTGAGATGGGAAGAAAATTAGATGAAGAAGATGATCAATGCATAGAAGCCAAAGGATATGAAGCATTAATGTTCTTTTCAGCATTATATTTGGTAGCATTGGGAAGTGGATGTTTAAAGCCAAACATAATATCACATGGGGCAGACCAATTTAGAAAAGAAGACCCAAAACAATTCAAAAAGCTATCAACTTTCTTCAATTGTGCTTATTTTGCATTTTGCACTGGTGAACTCATTGCATTGACTCTTCTTGTTTGGGTTCAAACACATTCTGGCATGGACCTTGGCTTTGGTGTTTCCACTGCTGCTATGCTTCTTGCTTTGATTTCATTGCTCTCTGGCACTTCTTTTTATAGGAATAATCCCCCGCGTGGCAGTATCTTCACTCCAATTGCTCAAGTCTTGGTTGCTGCATATAGGAAAAGGAACCTACAAATGTGTCCTTCGAATTCAGAGATGGTACTCAATGGAACTCAAAATGGCATTGTCAAACTCATACACACCGATAAGTTCAGATTCCTTGACAAGGCTTGCATAAAAAGTGAAGAAGAAAGATTGGGATCAATTAGAGAAGAAAGTCCATGGAAGCTTTGCACAGTAAAACAAGTAGAACAAGTGAAAATAATACTCTCAGTAATTCCCATATTTGCTTGCACCATAATCTTCAACACCATTTTAGCACAACTCCAAACATTCTCAGTCCAACAAGGAGCTTCCATGAACACATGGCTCACAAATTCCTTCCAAATCCCCCCAGCTTCCCTCCAAGCCATCCCTTACATTATCCTCATCTTCCTCGTCCCTCTTTACGAGACCGTCTTTGTCCCACTCACTCGTCGCCTCACCTCCATCGACTCCGGCATCTCCCCTCTCCAACGTGTCGGCACCGGCCTCTTTGTCGCCACCTTCTCCATGGTCTCTGCTGCACTGGTCGAGCAAAAACGTCGAAACTCTTCCTCCTCCCTCTCCATCCTCTGGATTGCACCACAATTCGTGATATTCGGCGTGTCAGAGCTGTTTACGGCGGTCGGGTTGATCGAGTTTTTCTATAAGCAATCTGTGGAAGGGATGCAGTCATTTTTAACTGCCATGACTTATTGTTCATACTCCTTCGGGTTCTACTTGAGCTCTTTGTTGGTGTCACTTGTGAATAAGATCAGTGGTGGATGGCTTAGCCATAATGACCTAAATAATGATAGGTTGGATCTGTTCTATTGGTTGTTGGCTGGTCTTAGCTTTGTGAATTTCTTCAACTATCTCTTTTGGGCCAATAGATTTTCTCAACAACCTCCTTTGCCACTTCATCTTCTTCAAACCCAAACCTCAACCAAACAACCTTCAAACTCTAAGCTTGTAGTGCCTTGA

Coding sequence (CDS)

ATGCAAAGAGATCATGAAGATATTTGTGTGGATTGGAGAGGGCGAGCTTGTAATCCTAACAGACATGGTGGAATGGGAGCTGCTGCCTTTGTCCTTGGACTTCAAGCATTTGAGATGATGGCAATAGCAGCAGTTGGGAATAATCTGATAACATATGTTTTCAGTGAAATGCATTTTCCACTGTCTAAATCAGCAAACGTTGTTACCAACTTTGTTGGAACTGTCTTCTTTTTGTCTCTTCTTGGTGGCTTCTTGTCCGATTCTTATCTTGGTAGCTTTCGAACTATGCTCATCTTTGGCTTCATCGAACTCGCTGGATTTACAGTTCTAACGATACAAGCTCATTACCCAGGACTAAGGCCATCACCATGTGAGATGGGAAGAAAATTAGATGAAGAAGATGATCAATGCATAGAAGCCAAAGGATATGAAGCATTAATGTTCTTTTCAGCATTATATTTGGTAGCATTGGGAAGTGGATGTTTAAAGCCAAACATAATATCACATGGGGCAGACCAATTTAGAAAAGAAGACCCAAAACAATTCAAAAAGCTATCAACTTTCTTCAATTGTGCTTATTTTGCATTTTGCACTGGTGAACTCATTGCATTGACTCTTCTTGTTTGGGTTCAAACACATTCTGGCATGGACCTTGGCTTTGGTGTTTCCACTGCTGCTATGCTTCTTGCTTTGATTTCATTGCTCTCTGGCACTTCTTTTTATAGGAATAATCCCCCGCGTGGCAGTATCTTCACTCCAATTGCTCAAGTCTTGGTTGCTGCATATAGGAAAAGGAACCTACAAATGTGTCCTTCGAATTCAGAGATGGTACTCAATGGAACTCAAAATGGCATTGTCAAACTCATACACACCGATAAGTTCAGATTCCTTGACAAGGCTTGCATAAAAAGTGAAGAAGAAAGATTGGGATCAATTAGAGAAGAAAGTCCATGGAAGCTTTGCACAGTAAAACAAGTAGAACAAGTGAAAATAATACTCTCAGTAATTCCCATATTTGCTTGCACCATAATCTTCAACACCATTTTAGCACAACTCCAAACATTCTCAGTCCAACAAGGAGCTTCCATGAACACATGGCTCACAAATTCCTTCCAAATCCCCCCAGCTTCCCTCCAAGCCATCCCTTACATTATCCTCATCTTCCTCGTCCCTCTTTACGAGACCGTCTTTGTCCCACTCACTCGTCGCCTCACCTCCATCGACTCCGGCATCTCCCCTCTCCAACGTGTCGGCACCGGCCTCTTTGTCGCCACCTTCTCCATGGTCTCTGCTGCACTGGTCGAGCAAAAACGTCGAAACTCTTCCTCCTCCCTCTCCATCCTCTGGATTGCACCACAATTCGTGATATTCGGCGTGTCAGAGCTGTTTACGGCGGTCGGGTTGATCGAGTTTTTCTATAAGCAATCTGTGGAAGGGATGCAGTCATTTTTAACTGCCATGACTTATTGTTCATACTCCTTCGGGTTCTACTTGAGCTCTTTGTTGGTGTCACTTGTGAATAAGATCAGTGGTGGATGGCTTAGCCATAATGACCTAAATAATGATAGGTTGGATCTGTTCTATTGGTTGTTGGCTGGTCTTAGCTTTGTGAATTTCTTCAACTATCTCTTTTGGGCCAATAGATTTTCTCAACAACCTCCTTTGCCACTTCATCTTCTTCAAACCCAAACCTCAACCAAACAACCTTCAAACTCTAAGCTTGTAGTGCCTTGA

Protein sequence

MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKACIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP
BLAST of CsaV3_5G039430 vs. NCBI nr
Match: XP_004141613.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.4 [Cucumis sativus] >KGN52605.1 hypothetical protein Csa_5G647280 [Cucumis sativus])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 1   MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP 60
           MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP
Sbjct: 1   MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP 60

Query: 61  LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR 120
           LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR
Sbjct: 61  LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR 120

Query: 121 PSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPK 180
           PSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPK
Sbjct: 121 PSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPK 180

Query: 181 QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF 240
           QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF
Sbjct: 181 QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF 240

Query: 241 YRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKA 300
           YRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKA
Sbjct: 241 YRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKA 300

Query: 301 CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 360
           CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG
Sbjct: 301 CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 360

Query: 361 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG 420
           ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG
Sbjct: 361 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG 420

Query: 421 LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM 480
           LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM
Sbjct: 421 LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM 480

Query: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 540
           QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF
Sbjct: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 540

Query: 541 NYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP 578
           NYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP
Sbjct: 541 NYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP 577

BLAST of CsaV3_5G039430 vs. NCBI nr
Match: XP_008459507.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.4 [Cucumis melo])

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 565/577 (97.92%), Postives = 569/577 (98.61%), Query Frame = 0

Query: 1   MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP 60
           MQRDHEDI VDWRGRACNPN+HGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP
Sbjct: 1   MQRDHEDIYVDWRGRACNPNKHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP 60

Query: 61  LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR 120
           LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR
Sbjct: 61  LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR 120

Query: 121 PSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPK 180
           PSPCEMGRKLDEE D+CIEAKGYEALMF+SALYLVALGSGCLKPNIISHGADQFRKEDPK
Sbjct: 121 PSPCEMGRKLDEE-DECIEAKGYEALMFYSALYLVALGSGCLKPNIISHGADQFRKEDPK 180

Query: 181 QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF 240
           QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF
Sbjct: 181 QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF 240

Query: 241 YRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKA 300
           YRNNPPRGSIFTPIAQVLVAAYRKRNLQ C SNSEMVLNGTQNGIVKLIHTDKFRFLDKA
Sbjct: 241 YRNNPPRGSIFTPIAQVLVAAYRKRNLQTCASNSEMVLNGTQNGIVKLIHTDKFRFLDKA 300

Query: 301 CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 360
           CI+SEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG
Sbjct: 301 CIRSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 360

Query: 361 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG 420
           ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPL RRLTSIDSGISPLQRVGTG
Sbjct: 361 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLARRLTSIDSGISPLQRVGTG 420

Query: 421 LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM 480
           LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM
Sbjct: 421 LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM 480

Query: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 540
           QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLN DRLDLFYWLLAGLSFVNFF
Sbjct: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNKDRLDLFYWLLAGLSFVNFF 540

Query: 541 NYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP 578
           NYLFWANRFSQQ PLPLHLLQTQTS KQPSNSKLVVP
Sbjct: 541 NYLFWANRFSQQQPLPLHLLQTQTS-KQPSNSKLVVP 575

BLAST of CsaV3_5G039430 vs. NCBI nr
Match: XP_022974070.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita maxima])

HSP 1 Score: 947.2 bits (2447), Expect = 2.6e-272
Identity = 485/550 (88.18%), Postives = 515/550 (93.64%), Query Frame = 0

Query: 7   DICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSAN 66
           D CVDWRGR CN N+HGGMGAAAFVLGLQAFEMMAIAAVGNNLITYV+SEMHFPLSK+AN
Sbjct: 15  DTCVDWRGRPCNSNKHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVYSEMHFPLSKAAN 74

Query: 67  VVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEM 126
           VVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGFIEL+GFTVLT+QAHYPGLRP+ CEM
Sbjct: 75  VVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFIELSGFTVLTVQAHYPGLRPAACEM 134

Query: 127 GRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLS 186
           GR    E D+C EAKGY+ALMFFSALYLVALGSGCLKPNIISHGADQF+K+DP QFKKLS
Sbjct: 135 GR--SGEGDECEEAKGYKALMFFSALYLVALGSGCLKPNIISHGADQFQKQDPTQFKKLS 194

Query: 187 TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPP 246
           TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF+RNNPP
Sbjct: 195 TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFFRNNPP 254

Query: 247 RGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKACIKSEE 306
           RGSIFTPIAQV VAA+ KR  Q+CPSNSEM L+G+ NG+VKL+HTD FRFLDKACIKSEE
Sbjct: 255 RGSIFTPIAQVFVAAFTKRK-QICPSNSEM-LHGSHNGVVKLLHTDNFRFLDKACIKSEE 314

Query: 307 ERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTW 366
            R G+ REESPWKLCTV QVEQVKIILSV+PIFACTIIFNTILAQLQTFSVQQGASMNT 
Sbjct: 315 TRAGT-REESPWKLCTVTQVEQVKIILSVVPIFACTIIFNTILAQLQTFSVQQGASMNTT 374

Query: 367 LTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFVATF 426
           LTNSFQIPPASLQ+IPY+ILIFLVPLYET FVPL RRLT+ DSGI+PLQRVGTGLFVATF
Sbjct: 375 LTNSFQIPPASLQSIPYLILIFLVPLYETAFVPLARRLTATDSGITPLQRVGTGLFVATF 434

Query: 427 SMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGMQSFLTA 486
           SMVSAALVEQKRR+ SS+LSILWIAPQFVIFG+SELFTAVGL+EFFYKQSVEGMQSFLTA
Sbjct: 435 SMVSAALVEQKRRH-SSNLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTA 494

Query: 487 MTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRLDLFYWLLAGLSFVNFFN 546
           MTYCSYSFGFYLSSLLVS+VNK+S     GGWLSHNDLN DRLDLFYWLLAGLS VNFFN
Sbjct: 495 MTYCSYSFGFYLSSLLVSVVNKVSRGESGGGWLSHNDLNKDRLDLFYWLLAGLSLVNFFN 554

Query: 547 YLFWANRFSQ 552
           YLFWANR+SQ
Sbjct: 555 YLFWANRYSQ 558

BLAST of CsaV3_5G039430 vs. NCBI nr
Match: XP_023536079.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 946.4 bits (2445), Expect = 4.4e-272
Identity = 485/550 (88.18%), Postives = 515/550 (93.64%), Query Frame = 0

Query: 7   DICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSAN 66
           D CVDWRGR CN N+HGGMGAAAFVLGLQAFEMMAIAAVGNNLITYV+SEMHFPLSK+AN
Sbjct: 15  DTCVDWRGRPCNSNKHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVYSEMHFPLSKAAN 74

Query: 67  VVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEM 126
           VVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGFIEL+GFTVLT+QAHYPGLRP+ CEM
Sbjct: 75  VVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFIELSGFTVLTVQAHYPGLRPAACEM 134

Query: 127 GRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLS 186
           GR    E D+C EAKGY+ALMFFSALYLVALGSGCLKPNIISHGADQF+K+DP QFKKLS
Sbjct: 135 GR--SGEGDECEEAKGYKALMFFSALYLVALGSGCLKPNIISHGADQFQKQDPTQFKKLS 194

Query: 187 TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPP 246
           TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF+RNNPP
Sbjct: 195 TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFFRNNPP 254

Query: 247 RGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKACIKSEE 306
           RGSIFTPIAQV VAA+ KR  Q+CPSNSEM L+G+ NG+VKL+HTD FRFLDKACIKSEE
Sbjct: 255 RGSIFTPIAQVFVAAFTKRK-QICPSNSEM-LHGSHNGVVKLLHTDNFRFLDKACIKSEE 314

Query: 307 ERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTW 366
            R G+ R+ESPWKLCTV QVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNT 
Sbjct: 315 TRAGT-RDESPWKLCTVTQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTT 374

Query: 367 LTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFVATF 426
           LTNSFQIPPASLQ+IPY+ILIFLVPLYET FVPL RRLT+ DSGI+PLQRVGTGLFVATF
Sbjct: 375 LTNSFQIPPASLQSIPYLILIFLVPLYETAFVPLARRLTATDSGITPLQRVGTGLFVATF 434

Query: 427 SMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGMQSFLTA 486
           SMVSAALVEQKRR+ SS+LSILWIAPQFVIFG+SELFTAVGL+EFFYKQSVEGMQSFLTA
Sbjct: 435 SMVSAALVEQKRRH-SSNLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTA 494

Query: 487 MTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRLDLFYWLLAGLSFVNFFN 546
           MTYCSYSFGFYLSSLLVS+VNK+S     GGWLSHNDLN DRLDLFYWLLAGLS VNFFN
Sbjct: 495 MTYCSYSFGFYLSSLLVSVVNKVSGGESGGGWLSHNDLNKDRLDLFYWLLAGLSLVNFFN 554

Query: 547 YLFWANRFSQ 552
           YLFWANR+SQ
Sbjct: 555 YLFWANRYSQ 558

BLAST of CsaV3_5G039430 vs. NCBI nr
Match: XP_022930188.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita moschata])

HSP 1 Score: 940.6 bits (2430), Expect = 2.4e-270
Identity = 483/550 (87.82%), Postives = 511/550 (92.91%), Query Frame = 0

Query: 7   DICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSAN 66
           D CVDWRGR CN N+HGGMGAAAFVLGLQAFEMMAIAAVGNNLITYV+SEMHFPLSK+AN
Sbjct: 15  DTCVDWRGRPCNSNKHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVYSEMHFPLSKAAN 74

Query: 67  VVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEM 126
           VVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGFIEL+GFTVLT+QAHYPGLRP+ CEM
Sbjct: 75  VVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFIELSGFTVLTVQAHYPGLRPAACEM 134

Query: 127 GRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLS 186
           G     E D+C EAKGY+ALMFFSALYLVALGSGCLKPNIISHGADQF+K+DP QFKKLS
Sbjct: 135 GS--SGEGDECEEAKGYKALMFFSALYLVALGSGCLKPNIISHGADQFQKQDPTQFKKLS 194

Query: 187 TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPP 246
           TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSG SF+RNNPP
Sbjct: 195 TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGASFFRNNPP 254

Query: 247 RGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKACIKSEE 306
           RGSIFTPIAQV VAA+ KR  Q+CPSNSEM L+G+ NG+VKL+HTD FRFLDKACIKSEE
Sbjct: 255 RGSIFTPIAQVFVAAFTKRK-QICPSNSEM-LHGSHNGVVKLLHTDNFRFLDKACIKSEE 314

Query: 307 ERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTW 366
            R G  REESPWKLCTV QVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNT 
Sbjct: 315 TR-GGTREESPWKLCTVTQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTT 374

Query: 367 LTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFVATF 426
           LTNSFQIPPASLQ+IPY+ILIFLVPLYET FVPL RRLT+ DSGI+PLQRVGTGLFVATF
Sbjct: 375 LTNSFQIPPASLQSIPYLILIFLVPLYETAFVPLARRLTATDSGITPLQRVGTGLFVATF 434

Query: 427 SMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGMQSFLTA 486
           SMVSAALVEQKRR+ SS+LSILWIAPQFVIFG+SELFTAVGL+EFFYKQSVEGMQSFLTA
Sbjct: 435 SMVSAALVEQKRRH-SSNLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTA 494

Query: 487 MTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRLDLFYWLLAGLSFVNFFN 546
           MTYCSYSFGFYLSSLLVS+VNK+S     GGWLSHNDLN DRLDLFYWLLAGLS VNF N
Sbjct: 495 MTYCSYSFGFYLSSLLVSVVNKVSGGGSGGGWLSHNDLNKDRLDLFYWLLAGLSLVNFIN 554

Query: 547 YLFWANRFSQ 552
           YLFWANR+SQ
Sbjct: 555 YLFWANRYSQ 558

BLAST of CsaV3_5G039430 vs. TAIR10
Match: AT1G33440.1 (Major facilitator superfamily protein)

HSP 1 Score: 762.7 bits (1968), Expect = 1.7e-220
Identity = 395/567 (69.66%), Postives = 460/567 (81.13%), Query Frame = 0

Query: 6   EDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 65
           +D+CVD+RGR C P++HGG  AA FVLG QAFEMMAIAAVGNNLITYVF+EMHFPLSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 66  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCE 125
           N+VTNF+GTVF LSLLGGFLSDSYLGSFRTML+FG IE++GF +L++QAH P LRP  C 
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 126 MGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKL 185
           M          C+EA GY+A   ++AL LVALGSGCLKPNIISHGA+QF+++D    +KL
Sbjct: 143 M----KSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKL 202

Query: 186 STFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNP 245
           S+FFN AYFAF  G+LIALTLLVWVQTHSGMD+GFGVS A M   +ISL++GTSFYRN P
Sbjct: 203 SSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKP 262

Query: 246 PRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQN--GIVKLIHTDKFRFLDKACIK 305
           P GSIFTPIAQV VAA  KR  Q+CPSN  MV   + +   +  L+H++KFRFLDKACIK
Sbjct: 263 PSGSIFTPIAQVFVAAITKRK-QICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIK 322

Query: 306 SEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASM 365
           ++ + +     ESPW+LCT++QV QVKI+LSVIPIFACTIIFNTILAQLQTFSVQQG+SM
Sbjct: 323 TQGKAM-----ESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSM 382

Query: 366 NTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFV 425
           NT +T +FQIPPASLQAIPYIILIF VPLYET FVPL R+LT  DSGISPLQR+GTGLF+
Sbjct: 383 NTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFL 442

Query: 426 ATFSMVSAALVEQKRRNS----SSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEG 485
           ATFSMV+AALVE+KRR S    +  LSI WIAPQF+IFG+SE+FTAVGL+EFFYKQS + 
Sbjct: 443 ATFSMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQS 502

Query: 486 MQSFLTAMTYCSYSFGFYLSSLLVSLVNKISG--------GWLSHNDLNNDRLDLFYWLL 545
           MQSFLTAMTYCSYSFGFYLSS+LVS VN+++         GWL  NDLN DRLD FYWLL
Sbjct: 503 MQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLL 562

Query: 546 AGLSFVNFFNYLFWANRFSQQPPLPLH 559
           A LSF+NFFNYLFW+  +S  P    H
Sbjct: 563 ASLSFINFFNYLFWSRWYSCDPSATHH 576

BLAST of CsaV3_5G039430 vs. TAIR10
Match: AT1G59740.1 (Major facilitator superfamily protein)

HSP 1 Score: 698.7 bits (1802), Expect = 2.9e-201
Identity = 367/557 (65.89%), Postives = 440/557 (78.99%), Query Frame = 0

Query: 6   EDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 65
           E+  VDWRGR  NPN+HGGM AA FVLGLQAFE+M IAAVGNNLITYV +EMHFPLSK+A
Sbjct: 26  EEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAA 85

Query: 66  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCE 125
           N+VTNFVGT+F  +LLGG+LSD++LGSF T++IFGF+EL+GF +L++QAH P L+P  C 
Sbjct: 86  NIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCN 145

Query: 126 MGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKL 185
                   D  C EAKG++A++FF ALYLVALGSGC+KPN+I+HGADQF +  PKQ K+L
Sbjct: 146 -----PLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRL 205

Query: 186 STFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNP 245
           S++FN AYFAF  GELIALTLLVWVQTHSGMD+GFGVS AAM + +ISL+SGT ++RN  
Sbjct: 206 SSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKR 265

Query: 246 PRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNG---TQNGIV---KLIHTDKFRFLDK 305
           PR SIFTPIA V+VAA  KR L   PS+  M L+G     N +V    L HT +FRFLDK
Sbjct: 266 PRRSIFTPIAHVIVAAILKRKL-ASPSDPRM-LHGDHHVANDVVPSSTLPHTPRFRFLDK 325

Query: 306 ACIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQ 365
           ACIK ++       +ESPW+LCTV QVEQVK ++S++PIFA TI+FNTILAQLQTFSVQQ
Sbjct: 326 ACIKIQDTN----TKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQ 385

Query: 366 GASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGT 425
           G+SMNT L+NSF IPPASLQAIPYI+LIFLVPLY++  VP  R+LT  +SGI PL R+G 
Sbjct: 386 GSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGI 445

Query: 426 GLFVATFSMVSAALVEQKRRNSS----SSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQ 485
           GLF++TFSMVSAA++E+KRR+SS      LSI WI PQF+IFG+SE+FTAVGLIEFFYKQ
Sbjct: 446 GLFLSTFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQ 505

Query: 486 SVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKI------SGGWLSHNDLNNDRLDLFYW 545
           S +GM+SFL A+TYCSYSFGFY SS+LVS+VNKI      S GWL  NDLN DRLDLFYW
Sbjct: 506 SAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYW 565

Query: 546 LLAGLSFVNFFNYLFWA 547
           LLA LS        FW+
Sbjct: 566 LLAVLSLXXXXXXXFWS 571

BLAST of CsaV3_5G039430 vs. TAIR10
Match: AT1G69850.1 (nitrate transporter 1:2)

HSP 1 Score: 409.8 bits (1052), Expect = 2.7e-114
Identity = 243/575 (42.26%), Postives = 343/575 (59.65%), Query Frame = 0

Query: 11  DWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 70
           DWR RA    RHGGM AA+FVL ++  E +A  A  +NL+ Y+   MH   SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 71  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEMGRKL 130
           F+GT F L+LLGGFLSD++  +F+  LI   IE  G  +LTIQA  P L P  C      
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC------ 134

Query: 131 DEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLSTFFN 190
             +   C E  G +A M F  LYLVALG G +K ++ SHGA+QF +  PK  K+ STFFN
Sbjct: 135 --DSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN 194

Query: 191 CAYFAFC--TGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPPRG 250
             YF FC   G L+A+T +VW++ + G + GFGVST A+ ++++  LSG+ FYRN  P G
Sbjct: 195 --YFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCG 254

Query: 251 SIFTPIAQVLVAAYRK-----------RNLQMCPSNSEMVLNGTQNGIVKLIHTDKFR-- 310
           S  T I +VL+AA  K            ++ + PSN   V  G +  +      +K R  
Sbjct: 255 SPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSN-HCVSKGKKE-VESQGELEKPRQE 314

Query: 311 --FLDKACIKSEEERLGSIREESPWKL---CTVKQVEQVKIILSVIPIFACTIIFNTILA 370
                +A + +  + L    +E P      CTV+QVE VKI+L ++PIFACTI+ N  LA
Sbjct: 315 EALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLA 374

Query: 371 QLQTFSVQQGASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSG 430
           QL TFSVQQ ASMNT +  S +IPPASL   P + ++ L P+Y+ + +P  R+ T  ++G
Sbjct: 375 QLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETG 434

Query: 431 ISPLQRVGTGLFVATFSMVSAALVEQKRRNSSSS-----------LSILWIAPQFVIFGV 490
           ++ LQR+G GL ++  +M  AALVE KR+  +             ++ LWIA Q++  G 
Sbjct: 435 VTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGS 494

Query: 491 SELFTAVGLIEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKISGG-----WL 550
           ++LFT  GL+E+F+ ++   M+S  T++++ S + G+YLSS++VS+VN I+G      WL
Sbjct: 495 ADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWL 554

BLAST of CsaV3_5G039430 vs. TAIR10
Match: AT1G27040.1 (Major facilitator superfamily protein)

HSP 1 Score: 392.5 bits (1007), Expect = 4.5e-109
Identity = 229/558 (41.04%), Postives = 333/558 (59.68%), Query Frame = 0

Query: 11  DWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 70
           DWR +A    RHGGM AA+FVL ++  E +A  A  +NL+ Y+ + MH  L++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 71  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEMGRKL 130
           F+ T F L+LLGGFL+D++  +F   LI   IE  G  +LTIQA  P L P PC+    L
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAAL 136

Query: 131 DEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLSTFFN 190
                +C    G +A   F  LYLV+LG G +K ++ SHGA+QF +  PK  K+ STFFN
Sbjct: 137 -----RCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 196

Query: 191 CAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPPRGSI 250
              F    G L+A+T +VW++ + G + GFGVST ++ L+++  L G+ FY+N  PRGS 
Sbjct: 197 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 256

Query: 251 FTPIAQVLVAAYRKRNLQMCPSN---SEMVLNGTQNGIVKLIHTDKFRFLDKACIKSEEE 310
            T I +VL+AA          SN   S  V +  +        T+    L+KA I+ +  
Sbjct: 257 LTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKA-IEGKTH 316

Query: 311 RLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTWL 370
            +        W  CTV+QVE VKI+L ++PIF CTI+ N  LAQL T+SV Q A+MN  +
Sbjct: 317 HI--------WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKI 376

Query: 371 TNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFVATFS 430
            N F +P ASL   P + ++ L P Y+ + +P  R++T  + GI+ LQR+G GL ++  +
Sbjct: 377 VN-FNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVA 436

Query: 431 MVSAALVEQKRR---------NSSSSLSI--LWIAPQFVIFGVSELFTAVGLIEFFYKQS 490
           M  AALVE KR+         +S  +L I  LWIA Q++  G ++LFT  GL+EFF+ ++
Sbjct: 437 MAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEA 496

Query: 491 VEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRLDLFYWLL 550
              M+S  T++++ S + G+YLSS++V +VN+++       WL    LN +RLDLFYWL+
Sbjct: 497 PSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLM 556

BLAST of CsaV3_5G039430 vs. TAIR10
Match: AT2G02040.1 (peptide transporter 2)

HSP 1 Score: 371.7 bits (953), Expect = 8.2e-103
Identity = 223/568 (39.26%), Postives = 327/568 (57.57%), Query Frame = 0

Query: 6   EDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 65
           ED  VD+ G      + G   A  F+LG +  E +A   +  NLITY+ +++H     +A
Sbjct: 26  EDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAA 85

Query: 66  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCE 125
             VT + GT +   L+G  L+D+Y G + T+  F  I   G + LT+ A  P L+P+ C 
Sbjct: 86  TNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAEC- 145

Query: 126 MGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKL 185
                    D C  A   +  MFF  LYL+ALG+G +KP + S GADQF   D ++  + 
Sbjct: 146 -------IGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRK 205

Query: 186 STFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNP 245
           ++FFN  YF+   G L++ +LLVW+Q + G  LGFG+ T  M LA+ S   GT  YR   
Sbjct: 206 ASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQK 265

Query: 246 PRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVL-----NGTQNGIVKLIHTDKFRFLDKA 305
           P GS  T I+QV+VA++RK ++++ P ++ ++      N    G  K+ HTD  ++LDKA
Sbjct: 266 PGGSPITRISQVVVASFRKSSVKV-PEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKA 325

Query: 306 CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 365
            + SEEE        S W+LCTV QVE++KI++ + PI+A  IIF+ + AQ+ T  VQQG
Sbjct: 326 AVISEEESKSGDYSNS-WRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQG 385

Query: 366 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG 425
            +MN  +  SFQ+PPA+L       +I  VPLY+   VPL R+ T +D G + +QR+G G
Sbjct: 386 RAMNCKI-GSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIG 445

Query: 426 LFVATFSMVSAALVEQKRRNSSSSL-----------SILWIAPQFVIFGVSELFTAVGLI 485
           LFV+   M +AA+VE  R + ++ L           S+LW  PQ+ I G +E+F  +G +
Sbjct: 446 LFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQL 505

Query: 486 EFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRL 545
           EFFY QS + M+S  +A+   + + G YLSSL+++LV   +      GW+S N LN+  L
Sbjct: 506 EFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDN-LNSGHL 565

Query: 546 DLFYWLLAGLSFVNFFNYLFWANRFSQQ 553
           D F+WLLAGLS VN   Y F A R+ Q+
Sbjct: 566 DYFFWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of CsaV3_5G039430 vs. Swiss-Prot
Match: sp|Q56XQ6|PTR15_ARATH (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 3.0e-219
Identity = 395/567 (69.66%), Postives = 460/567 (81.13%), Query Frame = 0

Query: 6   EDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 65
           +D+CVD+RGR C P++HGG  AA FVLG QAFEMMAIAAVGNNLITYVF+EMHFPLSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 66  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCE 125
           N+VTNF+GTVF LSLLGGFLSDSYLGSFRTML+FG IE++GF +L++QAH P LRP  C 
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 126 MGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKL 185
           M          C+EA GY+A   ++AL LVALGSGCLKPNIISHGA+QF+++D    +KL
Sbjct: 143 M----KSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKL 202

Query: 186 STFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNP 245
           S+FFN AYFAF  G+LIALTLLVWVQTHSGMD+GFGVS A M   +ISL++GTSFYRN P
Sbjct: 203 SSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKP 262

Query: 246 PRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQN--GIVKLIHTDKFRFLDKACIK 305
           P GSIFTPIAQV VAA  KR  Q+CPSN  MV   + +   +  L+H++KFRFLDKACIK
Sbjct: 263 PSGSIFTPIAQVFVAAITKRK-QICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIK 322

Query: 306 SEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASM 365
           ++ + +     ESPW+LCT++QV QVKI+LSVIPIFACTIIFNTILAQLQTFSVQQG+SM
Sbjct: 323 TQGKAM-----ESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSM 382

Query: 366 NTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFV 425
           NT +T +FQIPPASLQAIPYIILIF VPLYET FVPL R+LT  DSGISPLQR+GTGLF+
Sbjct: 383 NTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFL 442

Query: 426 ATFSMVSAALVEQKRRNS----SSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEG 485
           ATFSMV+AALVE+KRR S    +  LSI WIAPQF+IFG+SE+FTAVGL+EFFYKQS + 
Sbjct: 443 ATFSMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQS 502

Query: 486 MQSFLTAMTYCSYSFGFYLSSLLVSLVNKISG--------GWLSHNDLNNDRLDLFYWLL 545
           MQSFLTAMTYCSYSFGFYLSS+LVS VN+++         GWL  NDLN DRLD FYWLL
Sbjct: 503 MQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLL 562

Query: 546 AGLSFVNFFNYLFWANRFSQQPPLPLH 559
           A LSF+NFFNYLFW+  +S  P    H
Sbjct: 563 ASLSFINFFNYLFWSRWYSCDPSATHH 576

BLAST of CsaV3_5G039430 vs. Swiss-Prot
Match: sp|Q93VV5|PTR16_ARATH (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 5.3e-200
Identity = 367/557 (65.89%), Postives = 440/557 (78.99%), Query Frame = 0

Query: 6   EDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 65
           E+  VDWRGR  NPN+HGGM AA FVLGLQAFE+M IAAVGNNLITYV +EMHFPLSK+A
Sbjct: 26  EEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAA 85

Query: 66  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCE 125
           N+VTNFVGT+F  +LLGG+LSD++LGSF T++IFGF+EL+GF +L++QAH P L+P  C 
Sbjct: 86  NIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCN 145

Query: 126 MGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKL 185
                   D  C EAKG++A++FF ALYLVALGSGC+KPN+I+HGADQF +  PKQ K+L
Sbjct: 146 -----PLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRL 205

Query: 186 STFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNP 245
           S++FN AYFAF  GELIALTLLVWVQTHSGMD+GFGVS AAM + +ISL+SGT ++RN  
Sbjct: 206 SSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKR 265

Query: 246 PRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNG---TQNGIV---KLIHTDKFRFLDK 305
           PR SIFTPIA V+VAA  KR L   PS+  M L+G     N +V    L HT +FRFLDK
Sbjct: 266 PRRSIFTPIAHVIVAAILKRKL-ASPSDPRM-LHGDHHVANDVVPSSTLPHTPRFRFLDK 325

Query: 306 ACIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQ 365
           ACIK ++       +ESPW+LCTV QVEQVK ++S++PIFA TI+FNTILAQLQTFSVQQ
Sbjct: 326 ACIKIQDTN----TKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQ 385

Query: 366 GASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGT 425
           G+SMNT L+NSF IPPASLQAIPYI+LIFLVPLY++  VP  R+LT  +SGI PL R+G 
Sbjct: 386 GSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGI 445

Query: 426 GLFVATFSMVSAALVEQKRRNSS----SSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQ 485
           GLF++TFSMVSAA++E+KRR+SS      LSI WI PQF+IFG+SE+FTAVGLIEFFYKQ
Sbjct: 446 GLFLSTFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQ 505

Query: 486 SVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKI------SGGWLSHNDLNNDRLDLFYW 545
           S +GM+SFL A+TYCSYSFGFY SS+LVS+VNKI      S GWL  NDLN DRLDLFYW
Sbjct: 506 SAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYW 565

Query: 546 LLAGLSFVNFFNYLFWA 547
           LLA LS        FW+
Sbjct: 566 LLAVLSLXXXXXXXFWS 571

BLAST of CsaV3_5G039430 vs. Swiss-Prot
Match: sp|Q8H157|PTR19_ARATH (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.9e-113
Identity = 243/575 (42.26%), Postives = 343/575 (59.65%), Query Frame = 0

Query: 11  DWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 70
           DWR RA    RHGGM AA+FVL ++  E +A  A  +NL+ Y+   MH   SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 71  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEMGRKL 130
           F+GT F L+LLGGFLSD++  +F+  LI   IE  G  +LTIQA  P L P  C      
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC------ 134

Query: 131 DEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLSTFFN 190
             +   C E  G +A M F  LYLVALG G +K ++ SHGA+QF +  PK  K+ STFFN
Sbjct: 135 --DSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN 194

Query: 191 CAYFAFC--TGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPPRG 250
             YF FC   G L+A+T +VW++ + G + GFGVST A+ ++++  LSG+ FYRN  P G
Sbjct: 195 --YFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCG 254

Query: 251 SIFTPIAQVLVAAYRK-----------RNLQMCPSNSEMVLNGTQNGIVKLIHTDKFR-- 310
           S  T I +VL+AA  K            ++ + PSN   V  G +  +      +K R  
Sbjct: 255 SPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSN-HCVSKGKKE-VESQGELEKPRQE 314

Query: 311 --FLDKACIKSEEERLGSIREESPWKL---CTVKQVEQVKIILSVIPIFACTIIFNTILA 370
                +A + +  + L    +E P      CTV+QVE VKI+L ++PIFACTI+ N  LA
Sbjct: 315 EALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLA 374

Query: 371 QLQTFSVQQGASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSG 430
           QL TFSVQQ ASMNT +  S +IPPASL   P + ++ L P+Y+ + +P  R+ T  ++G
Sbjct: 375 QLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETG 434

Query: 431 ISPLQRVGTGLFVATFSMVSAALVEQKRRNSSSS-----------LSILWIAPQFVIFGV 490
           ++ LQR+G GL ++  +M  AALVE KR+  +             ++ LWIA Q++  G 
Sbjct: 435 VTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGS 494

Query: 491 SELFTAVGLIEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKISGG-----WL 550
           ++LFT  GL+E+F+ ++   M+S  T++++ S + G+YLSS++VS+VN I+G      WL
Sbjct: 495 ADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWL 554

BLAST of CsaV3_5G039430 vs. Swiss-Prot
Match: sp|Q8VYE4|PTR12_ARATH (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 8.1e-108
Identity = 229/558 (41.04%), Postives = 333/558 (59.68%), Query Frame = 0

Query: 11  DWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 70
           DWR +A    RHGGM AA+FVL ++  E +A  A  +NL+ Y+ + MH  L++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 71  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEMGRKL 130
           F+ T F L+LLGGFL+D++  +F   LI   IE  G  +LTIQA  P L P PC+    L
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAAL 136

Query: 131 DEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLSTFFN 190
                +C    G +A   F  LYLV+LG G +K ++ SHGA+QF +  PK  K+ STFFN
Sbjct: 137 -----RCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 196

Query: 191 CAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPPRGSI 250
              F    G L+A+T +VW++ + G + GFGVST ++ L+++  L G+ FY+N  PRGS 
Sbjct: 197 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 256

Query: 251 FTPIAQVLVAAYRKRNLQMCPSN---SEMVLNGTQNGIVKLIHTDKFRFLDKACIKSEEE 310
            T I +VL+AA          SN   S  V +  +        T+    L+KA I+ +  
Sbjct: 257 LTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKA-IEGKTH 316

Query: 311 RLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTWL 370
            +        W  CTV+QVE VKI+L ++PIF CTI+ N  LAQL T+SV Q A+MN  +
Sbjct: 317 HI--------WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKI 376

Query: 371 TNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFVATFS 430
            N F +P ASL   P + ++ L P Y+ + +P  R++T  + GI+ LQR+G GL ++  +
Sbjct: 377 VN-FNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVA 436

Query: 431 MVSAALVEQKRR---------NSSSSLSI--LWIAPQFVIFGVSELFTAVGLIEFFYKQS 490
           M  AALVE KR+         +S  +L I  LWIA Q++  G ++LFT  GL+EFF+ ++
Sbjct: 437 MAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEA 496

Query: 491 VEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRLDLFYWLL 550
              M+S  T++++ S + G+YLSS++V +VN+++       WL    LN +RLDLFYWL+
Sbjct: 497 PSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLM 556

BLAST of CsaV3_5G039430 vs. Swiss-Prot
Match: sp|P46032|PTR2_ARATH (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.5e-101
Identity = 223/568 (39.26%), Postives = 327/568 (57.57%), Query Frame = 0

Query: 6   EDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 65
           ED  VD+ G      + G   A  F+LG +  E +A   +  NLITY+ +++H     +A
Sbjct: 26  EDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAA 85

Query: 66  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCE 125
             VT + GT +   L+G  L+D+Y G + T+  F  I   G + LT+ A  P L+P+ C 
Sbjct: 86  TNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAEC- 145

Query: 126 MGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKL 185
                    D C  A   +  MFF  LYL+ALG+G +KP + S GADQF   D ++  + 
Sbjct: 146 -------IGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRK 205

Query: 186 STFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNP 245
           ++FFN  YF+   G L++ +LLVW+Q + G  LGFG+ T  M LA+ S   GT  YR   
Sbjct: 206 ASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQK 265

Query: 246 PRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVL-----NGTQNGIVKLIHTDKFRFLDKA 305
           P GS  T I+QV+VA++RK ++++ P ++ ++      N    G  K+ HTD  ++LDKA
Sbjct: 266 PGGSPITRISQVVVASFRKSSVKV-PEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKA 325

Query: 306 CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 365
            + SEEE        S W+LCTV QVE++KI++ + PI+A  IIF+ + AQ+ T  VQQG
Sbjct: 326 AVISEEESKSGDYSNS-WRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQG 385

Query: 366 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG 425
            +MN  +  SFQ+PPA+L       +I  VPLY+   VPL R+ T +D G + +QR+G G
Sbjct: 386 RAMNCKI-GSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIG 445

Query: 426 LFVATFSMVSAALVEQKRRNSSSSL-----------SILWIAPQFVIFGVSELFTAVGLI 485
           LFV+   M +AA+VE  R + ++ L           S+LW  PQ+ I G +E+F  +G +
Sbjct: 446 LFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQL 505

Query: 486 EFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRL 545
           EFFY QS + M+S  +A+   + + G YLSSL+++LV   +      GW+S N LN+  L
Sbjct: 506 EFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDN-LNSGHL 565

Query: 546 DLFYWLLAGLSFVNFFNYLFWANRFSQQ 553
           D F+WLLAGLS VN   Y F A R+ Q+
Sbjct: 566 DYFFWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of CsaV3_5G039430 vs. TrEMBL
Match: tr|A0A0A0KSN5|A0A0A0KSN5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G647280 PE=4 SV=1)

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 1   MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP 60
           MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP
Sbjct: 1   MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP 60

Query: 61  LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR 120
           LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR
Sbjct: 61  LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR 120

Query: 121 PSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPK 180
           PSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPK
Sbjct: 121 PSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPK 180

Query: 181 QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF 240
           QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF
Sbjct: 181 QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF 240

Query: 241 YRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKA 300
           YRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKA
Sbjct: 241 YRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKA 300

Query: 301 CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 360
           CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG
Sbjct: 301 CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 360

Query: 361 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG 420
           ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG
Sbjct: 361 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG 420

Query: 421 LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM 480
           LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM
Sbjct: 421 LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM 480

Query: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 540
           QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF
Sbjct: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 540

Query: 541 NYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP 578
           NYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP
Sbjct: 541 NYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP 577

BLAST of CsaV3_5G039430 vs. TrEMBL
Match: tr|A0A1S3CAU6|A0A1S3CAU6_CUCME (protein NRT1/ PTR FAMILY 4.4 OS=Cucumis melo OX=3656 GN=LOC103498622 PE=4 SV=1)

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 565/577 (97.92%), Postives = 569/577 (98.61%), Query Frame = 0

Query: 1   MQRDHEDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP 60
           MQRDHEDI VDWRGRACNPN+HGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP
Sbjct: 1   MQRDHEDIYVDWRGRACNPNKHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFP 60

Query: 61  LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR 120
           LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR
Sbjct: 61  LSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLR 120

Query: 121 PSPCEMGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPK 180
           PSPCEMGRKLDEE D+CIEAKGYEALMF+SALYLVALGSGCLKPNIISHGADQFRKEDPK
Sbjct: 121 PSPCEMGRKLDEE-DECIEAKGYEALMFYSALYLVALGSGCLKPNIISHGADQFRKEDPK 180

Query: 181 QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF 240
           QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF
Sbjct: 181 QFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSF 240

Query: 241 YRNNPPRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKA 300
           YRNNPPRGSIFTPIAQVLVAAYRKRNLQ C SNSEMVLNGTQNGIVKLIHTDKFRFLDKA
Sbjct: 241 YRNNPPRGSIFTPIAQVLVAAYRKRNLQTCASNSEMVLNGTQNGIVKLIHTDKFRFLDKA 300

Query: 301 CIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 360
           CI+SEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG
Sbjct: 301 CIRSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG 360

Query: 361 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTG 420
           ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPL RRLTSIDSGISPLQRVGTG
Sbjct: 361 ASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLARRLTSIDSGISPLQRVGTG 420

Query: 421 LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM 480
           LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM
Sbjct: 421 LFVATFSMVSAALVEQKRRNSSSSLSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGM 480

Query: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 540
           QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLN DRLDLFYWLLAGLSFVNFF
Sbjct: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKISGGWLSHNDLNKDRLDLFYWLLAGLSFVNFF 540

Query: 541 NYLFWANRFSQQPPLPLHLLQTQTSTKQPSNSKLVVP 578
           NYLFWANRFSQQ PLPLHLLQTQTS KQPSNSKLVVP
Sbjct: 541 NYLFWANRFSQQQPLPLHLLQTQTS-KQPSNSKLVVP 575

BLAST of CsaV3_5G039430 vs. TrEMBL
Match: tr|A0A2P6PKB8|A0A2P6PKB8_ROSCH (Putative proton-dependent oligopeptide transporter family, major facilitator superfamily OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6g0249481 PE=4 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 7.6e-236
Identity = 428/583 (73.41%), Postives = 484/583 (83.02%), Query Frame = 0

Query: 7   DICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSAN 66
           DI VDWRGR C P +HGGM AA FVLGLQ FEMMAIAAVGNNLITYV +EMHFPLSKSAN
Sbjct: 19  DISVDWRGRPCKPTKHGGMKAAVFVLGLQGFEMMAIAAVGNNLITYVLNEMHFPLSKSAN 78

Query: 67  VVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEM 126
           +VTNF+GTVF LSL GGFLSDSYLGSF TMLIFGF+EL+GF +L++QAH P LRP  C++
Sbjct: 79  IVTNFIGTVFLLSLFGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLRPPSCKV 138

Query: 127 GRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLS 186
                   + C EAKGY+AL+FF+A+YLVALGSGCLKPNIISHGADQFRKED KQ KKLS
Sbjct: 139 -----MSSEYCQEAKGYKALIFFAAIYLVALGSGCLKPNIISHGADQFRKEDTKQSKKLS 198

Query: 187 TFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPP 246
           T+FNCAYFAFC GELIALT+LVWVQTHSGMD GFGVS AAM L LISL+ GTS YRN PP
Sbjct: 199 TYFNCAYFAFCMGELIALTVLVWVQTHSGMDAGFGVSAAAMTLGLISLVFGTSLYRNKPP 258

Query: 247 RGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQN------------GIVKLIHTDKF 306
           RGSIFTPIAQV VAA+ KR  Q+CPSN+EM L+G QN             +  L+HT+KF
Sbjct: 259 RGSIFTPIAQVFVAAFTKRK-QICPSNTEM-LHGNQNNVPNHLFVTMSPNVTTLLHTEKF 318

Query: 307 RFLDKACIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQT 366
           RFLDKACIK+E+   G+ R ESPW+LCTV QVEQVKII+SV+PIFACTIIFNTILAQLQT
Sbjct: 319 RFLDKACIKTED---GTERTESPWRLCTVSQVEQVKIIISVVPIFACTIIFNTILAQLQT 378

Query: 367 FSVQQGASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPL 426
           FSVQQG++MN  +T SF+IPPASLQAIPYI+LIFLVPLYETVFVP  R++T  DSGISPL
Sbjct: 379 FSVQQGSTMNARITESFKIPPASLQAIPYIMLIFLVPLYETVFVPTARKVTGRDSGISPL 438

Query: 427 QRVGTGLFVATFSMVSAALVEQKRRNS---SSSLSILWIAPQFVIFGVSELFTAVGLIEF 486
           QRVGTGLF+ATFSMVSAA++E+KRRNS   + +LSI WIAPQF+IFG+SE+FTAVGLIEF
Sbjct: 439 QRVGTGLFIATFSMVSAAVIERKRRNSALHNEALSIFWIAPQFLIFGLSEMFTAVGLIEF 498

Query: 487 FYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRLDL 546
           FYKQS+EGMQSFLTAMTYCSYSFGFYLSSLLVSLVNK++     GGWLS NDLN DRLD 
Sbjct: 499 FYKQSLEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKVTSTSSRGGWLSDNDLNKDRLDR 558

Query: 547 FYWLLAGLSFVNFFNYLFWANRFSQQPPLPLHLLQTQTSTKQP 570
           FYWLLA LS +NFFNYLFW+N +   P L      + T+T  P
Sbjct: 559 FYWLLAALSLMNFFNYLFWSNWYCYNPTL------SPTNTNSP 585

BLAST of CsaV3_5G039430 vs. TrEMBL
Match: tr|A0A2R6Q1A6|A0A2R6Q1A6_ACTCH (Protein NRT1/ PTR FAMILY 4.4 like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc24158 PE=4 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.1e-234
Identity = 426/578 (73.70%), Postives = 479/578 (82.87%), Query Frame = 0

Query: 6   EDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 65
           E+I +DWRGR CN N HGGM AAAFVLGLQAFEMMAIAAVGNNLITYV ++MHFPLSKSA
Sbjct: 38  EEISIDWRGRPCNSNNHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVLNDMHFPLSKSA 97

Query: 66  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCE 125
           N+VTNF+GTVF LSLLGGFLSDSYLGSF TMLIFGF+EL GF +L++QAH P LRP  C 
Sbjct: 98  NIVTNFIGTVFLLSLLGGFLSDSYLGSFCTMLIFGFVELTGFILLSVQAHLPQLRPPHCN 157

Query: 126 MGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKL 185
           M   +    D C EAKGY+AL+F+ ALYLVALGSGCLKPNIISHGADQFRKED  Q KKL
Sbjct: 158 M---MSVRGDLCPEAKGYKALIFYVALYLVALGSGCLKPNIISHGADQFRKEDSNQSKKL 217

Query: 186 STFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNP 245
           ST+FNCAYFAFCTGEL+ALTLLVWVQTHSGMD+GFGVS AAM + LISL+SGT  Y+N P
Sbjct: 218 STYFNCAYFAFCTGELVALTLLVWVQTHSGMDVGFGVSAAAMAIGLISLISGTLVYKNKP 277

Query: 246 PRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMV----------LNGTQNGIVKLIHTDKFR 305
           PRGSIFTPIAQV VAA  KR  Q+CPS+SEM+           + T +    L HT+KFR
Sbjct: 278 PRGSIFTPIAQVFVAAITKRK-QICPSSSEMLHGSQSIESGCASTTSSNRSNLRHTEKFR 337

Query: 306 FLDKACIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTF 365
           FLDKACIK ++   GS   ESPW+LCTV QVEQVKI++SVIPIFACTIIFNTILAQLQTF
Sbjct: 338 FLDKACIKIQD---GSQMSESPWRLCTVTQVEQVKILISVIPIFACTIIFNTILAQLQTF 397

Query: 366 SVQQGASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQ 425
           SVQQG+SMNT L N+FQIPPASLQ+IPY++LIF+VPLYETVFVP+ R++T  DSGISPLQ
Sbjct: 398 SVQQGSSMNTSLPNNFQIPPASLQSIPYLMLIFVVPLYETVFVPIARKITGRDSGISPLQ 457

Query: 426 RVGTGLFVATFSMVSAALVEQKRRNSS----SSLSILWIAPQFVIFGVSELFTAVGLIEF 485
           RVG GLF+ATFSMVSAA++E KRRNSS     +LSI WIAPQF+IFG+SE+FTAVGLIEF
Sbjct: 458 RVGIGLFIATFSMVSAAVIENKRRNSSLNLNKTLSIFWIAPQFLIFGLSEMFTAVGLIEF 517

Query: 486 FYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIS--------GGWLSHNDLNNDR 545
           FYKQS+EGMQSFLTAMTYCSYSFGFYLSS+LVSLVNKI+        GGWLS NDLN DR
Sbjct: 518 FYKQSLEGMQSFLTAMTYCSYSFGFYLSSVLVSLVNKITSSSRSSSGGGWLSDNDLNKDR 577

Query: 546 LDLFYWLLAGLSFVNFFNYLFWANRFSQQP---PLPLH 559
           LDLFYWLLA LS +NFFNYLFW+  +S  P   P P H
Sbjct: 578 LDLFYWLLAALSLINFFNYLFWSRWYSYNPSLSPSPTH 608

BLAST of CsaV3_5G039430 vs. TrEMBL
Match: tr|M5VNK8|M5VNK8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_8G001900 PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 4.3e-231
Identity = 417/573 (72.77%), Postives = 473/573 (82.55%), Query Frame = 0

Query: 6   EDICVDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 65
           ED+ VDWRGR C P +HGGM AA FVLGLQ FEMMAIAAVGNNLITYV +EMHF LSKSA
Sbjct: 16  EDMSVDWRGRPCKPTKHGGMKAAVFVLGLQGFEMMAIAAVGNNLITYVLNEMHFSLSKSA 75

Query: 66  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCE 125
           N+VTNF+GTVF LSL GG+LSDSYLGSF TM+IFG +EL+GF +L +QAH P LRP PC+
Sbjct: 76  NIVTNFIGTVFLLSLFGGYLSDSYLGSFWTMIIFGLVELSGFMLLCVQAHLPELRPPPCK 135

Query: 126 MGRKLDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKL 185
           +        + C EAKGY+AL+FF+A+YLVALGSGCLKPNIISHGADQFR++D KQ KKL
Sbjct: 136 I-----MSHEYCPEAKGYKALIFFAAIYLVALGSGCLKPNIISHGADQFRRDDSKQSKKL 195

Query: 186 STFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNP 245
           S++FNCAYFAFC GELIALT+LVWVQTHSGMD+GFGVS AAM + LI L  GT  YRN P
Sbjct: 196 SSYFNCAYFAFCIGELIALTVLVWVQTHSGMDVGFGVSAAAMAMGLICLTFGTPLYRNKP 255

Query: 246 PRGSIFTPIAQVLVAAYRKRNLQMCPSNSEMVLNGTQN------------GIVKLIHTDK 305
           PRGSIFTPIAQV VAA+ KR  Q+CPSN+EM L+G QN             I  L+HT+K
Sbjct: 256 PRGSIFTPIAQVFVAAFTKRK-QICPSNTEM-LHGNQNNVPNHLFVPMSPNISPLLHTEK 315

Query: 306 FRFLDKACIKSEEERLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQ 365
           FRFLDKACIK E+   G+ R ESPW+LCTV QVEQVKII+SV+PIFACTIIFNTILAQLQ
Sbjct: 316 FRFLDKACIKIED---GTQRNESPWRLCTVAQVEQVKIIISVVPIFACTIIFNTILAQLQ 375

Query: 366 TFSVQQGASMNTWLTNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISP 425
           TFSVQQ  +MNT +T  F+IPPASLQAIPYI+LIF+VPLYE VFVP  R+LT  DSGISP
Sbjct: 376 TFSVQQATAMNTRITRGFKIPPASLQAIPYIMLIFVVPLYEIVFVPAARKLTGRDSGISP 435

Query: 426 LQRVGTGLFVATFSMVSAALVEQKRRN----SSSSLSILWIAPQFVIFGVSELFTAVGLI 485
           LQRVGTGLF+ATFSMVSAAL+EQKRRN    S+  LSI WIAPQF+IFG+SE+FTAVGLI
Sbjct: 436 LQRVGTGLFIATFSMVSAALIEQKRRNLALSSNEPLSIFWIAPQFLIFGLSEMFTAVGLI 495

Query: 486 EFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIS-----GGWLSHNDLNNDRL 545
           EFFYKQS+EGMQSFLTAMTYCSYSFGFYLSS+LVS+VNK++     GGWLS NDLN DRL
Sbjct: 496 EFFYKQSLEGMQSFLTAMTYCSYSFGFYLSSILVSVVNKLTSTSSRGGWLSDNDLNRDRL 555

Query: 546 DLFYWLLAGLSFVNFFNYLFWANRFSQQPPLPL 558
           D FYWLLA LS +NFFNYLFW+N +S  P L L
Sbjct: 556 DRFYWLLAALSLINFFNYLFWSNWYSYNPSLSL 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004141613.10.0e+00100.00PREDICTED: protein NRT1/ PTR FAMILY 4.4 [Cucumis sativus] >KGN52605.1 hypothetic... [more]
XP_008459507.10.0e+0097.92PREDICTED: protein NRT1/ PTR FAMILY 4.4 [Cucumis melo][more]
XP_022974070.12.6e-27288.18protein NRT1/ PTR FAMILY 4.4-like [Cucurbita maxima][more]
XP_023536079.14.4e-27288.18protein NRT1/ PTR FAMILY 4.4-like [Cucurbita pepo subsp. pepo][more]
XP_022930188.12.4e-27087.82protein NRT1/ PTR FAMILY 4.4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G33440.11.7e-22069.66Major facilitator superfamily protein[more]
AT1G59740.12.9e-20165.89Major facilitator superfamily protein[more]
AT1G69850.12.7e-11442.26nitrate transporter 1:2[more]
AT1G27040.14.5e-10941.04Major facilitator superfamily protein[more]
AT2G02040.18.2e-10339.26peptide transporter 2[more]
Match NameE-valueIdentityDescription
sp|Q56XQ6|PTR15_ARATH3.0e-21969.66Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
sp|Q93VV5|PTR16_ARATH5.3e-20065.89Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
sp|Q8H157|PTR19_ARATH4.9e-11342.26Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
sp|Q8VYE4|PTR12_ARATH8.1e-10841.04Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
sp|P46032|PTR2_ARATH1.5e-10139.26Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0KSN5|A0A0A0KSN5_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G647280 PE=4 SV=1[more]
tr|A0A1S3CAU6|A0A1S3CAU6_CUCME0.0e+0097.92protein NRT1/ PTR FAMILY 4.4 OS=Cucumis melo OX=3656 GN=LOC103498622 PE=4 SV=1[more]
tr|A0A2P6PKB8|A0A2P6PKB8_ROSCH7.6e-23673.41Putative proton-dependent oligopeptide transporter family, major facilitator sup... [more]
tr|A0A2R6Q1A6|A0A2R6Q1A6_ACTCH1.1e-23473.70Protein NRT1/ PTR FAMILY 4.4 like OS=Actinidia chinensis var. chinensis OX=15908... [more]
tr|M5VNK8|M5VNK8_PRUPE4.3e-23172.77Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_8G001900 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: Biological Process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR000109POT_fam
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006810 transport
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity
molecular_function GO:0005215 transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G039430.1CsaV3_5G039430.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 93..510
e-value: 1.2E-96
score: 323.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 5..558
NoneNo IPR availablePANTHERPTHR11654:SF198PROTEIN NRT1/ PTR FAMILY 4.4coord: 5..558
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 307..549
coord: 26..119
coord: 148..246