CsaV3_5G037580 (gene) Cucumber (Chinese Long) v3

NameCsaV3_5G037580
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
Descriptionmetalloendoproteinase 2-MMP-like
Locationchr5 : 29762682 .. 29763964 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTACATTTTATCTTCTATCCATATATAATTTTGCGTGCATTTTTCATCTATATTACAACAATTTGGACTTTTTATGACAGAAAAATAAAGACAAATTAGGAGAGAGAAAAGTCTGCAATAAAACTTAAACTGACTTTTTTTCTGTGAAAAAAACAATTCGTTATCAAAATTTTCAAAATTTACTAAAAATTTTTATGTCACAAATTTACGATGGTATAAATAATTATCATTAAAGTTGGATTATAACAAGCTAGATTTTCTATTTTGATAGCACGTTAATTTGATTGTTGAATTTAAACTTATTCTAAATCTTTTATTCACATTCTTAACAGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCTATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

mRNA sequence

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCTATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

Coding sequence (CDS)

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCTATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

Protein sequence

MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKGLDADDIAGINALYNAN
BLAST of CsaV3_5G037580 vs. NCBI nr
Match: XP_004142461.1 (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus] >KGN52414.1 hypothetical protein Csa_5G632130 [Cucumis sativus])

HSP 1 Score: 644.8 bits (1662), Expect = 1.5e-181
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA
Sbjct: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG
Sbjct: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of CsaV3_5G037580 vs. NCBI nr
Match: XP_008458907.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 576.6 bits (1485), Expect = 5.1e-161
Identity = 277/316 (87.66%), Postives = 294/316 (93.04%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FETLLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNN       SFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNXXXXXXXSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASK HLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVA +GRAFKTWGA+THFNFS+ S+YKKADIKISF +GDHGDGHSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of CsaV3_5G037580 vs. NCBI nr
Match: XP_022938357.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 481.5 bits (1238), Expect = 2.2e-132
Identity = 234/313 (74.76%), Postives = 267/313 (85.30%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MA    LSFL +S FL+I LFPLISSSH     S+GL+FL NLNGCKKGD VEG+HQ+K 
Sbjct: 1   MAVLQALSFLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YL+ FGYLN  VQN+SK++ DDEFD VLESAI+TYQLNYNL+ATGT++A TL LMS+PRC
Sbjct: 61  YLRRFGYLNR-VQNHSKTY-DDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G AD+  GKTRM SGKKMVN+HRKI+G FH VSH+AFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           ++A+A +GRAF TW ANTHF FS  S Y+ ADIKISFE+GDHGDG +FDGV GVIAH F+
Sbjct: 181 TEAIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFS 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E WA+GAI DSFD+ET+ALHEIGHLLGL HSS+E AIMWP+IMEG+TKG
Sbjct: 241 PTDGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKG 300

Query: 301 LDADDIAGINALY 314
           L ADDIAGINALY
Sbjct: 301 LHADDIAGINALY 311

BLAST of CsaV3_5G037580 vs. NCBI nr
Match: XP_023551500.1 (metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.8 bits (1231), Expect = 1.4e-131
Identity = 230/307 (74.92%), Postives = 264/307 (85.99%), Query Frame = 0

Query: 7   LSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFG 66
           LSF  +S FL+I LFPLISSSH     S+GL+FL NLNGCKKGD VEG+HQ+K YL  FG
Sbjct: 6   LSFFPSSLFLLITLFPLISSSHQYPAHSHGLLFLNNLNGCKKGDTVEGLHQMKRYLHRFG 65

Query: 67  YLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIV 126
           YL N+VQN+SK++ DDEFD +LESAI+TYQLNYNL+ATGT++A TL LMS+PRCG AD+ 
Sbjct: 66  YL-NHVQNHSKTY-DDEFDELLESAIQTYQLNYNLEATGTLDASTLALMSKPRCGVADVF 125

Query: 127 DGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTPSKAVAT 186
            GKTRM SGKKMVN+HRKI G FH VSH+AFFDGNPKWPASKSHLTYGFLPGTP++A+A 
Sbjct: 126 HGKTRMNSGKKMVNRHRKIGGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAIAP 185

Query: 187 IGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRL 246
           +GRAF TW ANTHF FSQ S Y+ ADIKISFE+GDHGDG +FDGV GVIAH F+P DGRL
Sbjct: 186 VGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGRL 245

Query: 247 HFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKGLDADDI 306
           HFDA E WA+GA+ DSFD+ET+ALHEIGHLLGL HSS+E AIMWP+IMEG+TKGL ADDI
Sbjct: 246 HFDAVEPWAIGAVPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHADDI 305

Query: 307 AGINALY 314
           AGINALY
Sbjct: 306 AGINALY 310

BLAST of CsaV3_5G037580 vs. NCBI nr
Match: XP_022993647.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 468.4 bits (1204), Expect = 1.9e-128
Identity = 228/315 (72.38%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MA    LSF  +S FL+I LFPLISSS      S+GL+FL NLNGCKKGD VEG+HQ+K 
Sbjct: 1   MAVLQALSFFASSLFLLITLFPLISSSPQYPAHSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YL  FGYL ++VQN+SK++ DDEFD +LESAI+TYQLNYNL+ATGT++A TL LMS+PRC
Sbjct: 61  YLCRFGYL-SHVQNHSKTY-DDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G AD+  GKT M SGKKMVN+HRKI+ HFH VSH+AFFDGNPKWPASKSHL YGFLPGTP
Sbjct: 121 GVADVFHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
            +A+A +GRAF TW ANTHF FSQ S Y+ ADIKISFE+GDHGDG +FDGV GVIAH F+
Sbjct: 181 IEAIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFS 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E+WA+GA+ +SFD+ET+ALHEIGHLLGL+HSS+E AIMWP+IMEG+TKG
Sbjct: 241 PTDGRLHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKG 300

Query: 301 LDADDIAGINALYNA 316
           L ADDIAGINALY A
Sbjct: 301 LHADDIAGINALYTA 313

BLAST of CsaV3_5G037580 vs. TAIR10
Match: AT1G70170.1 (matrix metalloproteinase)

HSP 1 Score: 243.4 bits (620), Expect = 1.8e-64
Identity = 127/305 (41.64%), Postives = 178/305 (58.36%), Query Frame = 0

Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP--SKAVATIGRAFKTWGANTH 204
                 H H+V  Y  F G P+WP ++  LTY F P  P   +  +   RAF  W   T 
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTA 205

Query: 205 FNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAI 264
            NF+ +  +  +DI I F TGDHGDG  FDGV G +AH F+P  G+ H DA E+W V   
Sbjct: 206 LNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGD 265

Query: 265 ADSF-------DLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINA 317
            DSF       DLE++A+HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ GI  
Sbjct: 266 LDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQY 325

BLAST of CsaV3_5G037580 vs. TAIR10
Match: AT1G24140.1 (Matrixin family protein)

HSP 1 Score: 230.7 bits (587), Expect = 1.2e-60
Identity = 118/287 (41.11%), Postives = 168/287 (58.54%), Query Frame = 0

Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F DD FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTDD-FDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKING-HFHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTYGFLP--GTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFE 221
            P+WP ++  LTY F P      +  +   RAF  W   T   F++  R+  +DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 222 TGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVG--------AIADSFDLETIAL 281
           +G+HGDG  FDG    +AH F+P  G  H D  E+W V         +++++ DLE++A+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 282 HEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ G+  LY AN
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGAN 331

BLAST of CsaV3_5G037580 vs. TAIR10
Match: AT1G59970.1 (Matrixin family protein)

HSP 1 Score: 212.2 bits (539), Expect = 4.6e-55
Identity = 124/327 (37.92%), Postives = 170/327 (51.99%), Query Frame = 0

Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLP--GT 184
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY F P    
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 186

Query: 185 PSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGF 244
             +      RAF  W   T  NF+++    +ADI I F +G+HGDG  FDG  G +AH  
Sbjct: 187 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 246

Query: 245 APRDGRLHFDAAESWAVG---------AIADSFDLETIALHEIGHLLGLYHSSIERAIMW 304
           +P  G LH D  E W +           +    DLE++A+HEIGHLLGL HSS+E AIM+
Sbjct: 247 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 306

Query: 305 PSIMEGSTK-GLDADDIAGINALYNAN 317
           P+I  G  K  L  DDI GI  LY  N
Sbjct: 307 PAISGGDRKVELAKDDIEGIQHLYGGN 316

BLAST of CsaV3_5G037580 vs. TAIR10
Match: AT4G16640.1 (Matrixin family protein)

HSP 1 Score: 205.7 bits (522), Expect = 4.3e-53
Identity = 121/281 (43.06%), Postives = 158/281 (56.23%), Query Frame = 0

Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--N 183

Query: 169 KSHLTYGF-----LPGTPSKAVATI-GRAFKTWGANTHFNFSQTSRYKKADIKISFETGD 228
           +  LTY       L    S+ V T+  RAF  W +    +F +   +  AD+KI F  GD
Sbjct: 184 RDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGD 243

Query: 229 HGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAV-----GAIADSFDLETIALHEIGHL 288
           HGDG  FDGV G +AH FAP +GRLH DAAE+W V     G+   + DLE++A HEIGHL
Sbjct: 244 HGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHL 303

Query: 289 LGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           LGL HSS E A+M+PS+   + K  L  DD+AG+  LY  N
Sbjct: 304 LGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPN 321

BLAST of CsaV3_5G037580 vs. TAIR10
Match: AT2G45040.1 (Matrixin family protein)

HSP 1 Score: 176.4 bits (446), Expect = 2.8e-44
Identity = 108/274 (39.42%), Postives = 144/274 (52.55%), Query Frame = 0

Query: 55  IHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDL 114
           I ++K +LQ +GYL       +K  DD  F    E A+  YQ N  L  TG  ++ TL  
Sbjct: 50  IPEIKRHLQQYGYLPQ-----NKESDDVSF----EQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 115 MSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPAS-KSHLTY 174
           +  PRCGF D V+ KT                  FH+   Y +F G P+W       LTY
Sbjct: 110 ILLPRCGFPDDVEPKT----------------APFHTGKKYVYFPGRPRWTRDVPLKLTY 169

Query: 175 GFLPG------TPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHS 234
            F          P+       RAF  W +    +F +T  Y  ADIKI F  GDHGDG  
Sbjct: 170 AFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEP 229

Query: 235 FDGVRGVIAHGFAPRDGRLHFDAAESWAVGAIAD----SFDLETIALHEIGHLLGLYHSS 294
           FDGV GV+AH F+P +GRLH D AE+WAV    +    + DLE++A+HEIGH+LGL HSS
Sbjct: 230 FDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSS 289

Query: 295 IERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           ++ A M+P++   S K  L+ DD+ G+ +LY  N
Sbjct: 290 VKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTN 298

BLAST of CsaV3_5G037580 vs. Swiss-Prot
Match: sp|O04529|2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.3e-63
Identity = 127/305 (41.64%), Postives = 178/305 (58.36%), Query Frame = 0

Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP--SKAVATIGRAFKTWGANTH 204
                 H H+V  Y  F G P+WP ++  LTY F P  P   +  +   RAF  W   T 
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTA 205

Query: 205 FNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAI 264
            NF+ +  +  +DI I F TGDHGDG  FDGV G +AH F+P  G+ H DA E+W V   
Sbjct: 206 LNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGD 265

Query: 265 ADSF-------DLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINA 317
            DSF       DLE++A+HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ GI  
Sbjct: 266 LDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQY 325

BLAST of CsaV3_5G037580 vs. Swiss-Prot
Match: sp|Q5XF51|3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.2e-59
Identity = 118/287 (41.11%), Postives = 168/287 (58.54%), Query Frame = 0

Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F DD FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTDD-FDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKING-HFHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTYGFLP--GTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFE 221
            P+WP ++  LTY F P      +  +   RAF  W   T   F++  R+  +DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 222 TGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVG--------AIADSFDLETIAL 281
           +G+HGDG  FDG    +AH F+P  G  H D  E+W V         +++++ DLE++A+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 282 HEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ G+  LY AN
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGAN 331

BLAST of CsaV3_5G037580 vs. Swiss-Prot
Match: sp|Q9ZUJ5|5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.2e-54
Identity = 124/327 (37.92%), Postives = 170/327 (51.99%), Query Frame = 0

Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLP--GT 184
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY F P    
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 186

Query: 185 PSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGF 244
             +      RAF  W   T  NF+++    +ADI I F +G+HGDG  FDG  G +AH  
Sbjct: 187 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 246

Query: 245 APRDGRLHFDAAESWAVG---------AIADSFDLETIALHEIGHLLGLYHSSIERAIMW 304
           +P  G LH D  E W +           +    DLE++A+HEIGHLLGL HSS+E AIM+
Sbjct: 247 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 306

Query: 305 PSIMEGSTK-GLDADDIAGINALYNAN 317
           P+I  G  K  L  DDI GI  LY  N
Sbjct: 307 PAISGGDRKVELAKDDIEGIQHLYGGN 316

BLAST of CsaV3_5G037580 vs. Swiss-Prot
Match: sp|O23507|1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 7.7e-52
Identity = 121/281 (43.06%), Postives = 158/281 (56.23%), Query Frame = 0

Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--N 183

Query: 169 KSHLTYGF-----LPGTPSKAVATI-GRAFKTWGANTHFNFSQTSRYKKADIKISFETGD 228
           +  LTY       L    S+ V T+  RAF  W +    +F +   +  AD+KI F  GD
Sbjct: 184 RDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGD 243

Query: 229 HGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAV-----GAIADSFDLETIALHEIGHL 288
           HGDG  FDGV G +AH FAP +GRLH DAAE+W V     G+   + DLE++A HEIGHL
Sbjct: 244 HGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHL 303

Query: 289 LGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           LGL HSS E A+M+PS+   + K  L  DD+AG+  LY  N
Sbjct: 304 LGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPN 321

BLAST of CsaV3_5G037580 vs. Swiss-Prot
Match: sp|P29136|MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 200.3 bits (508), Expect = 3.2e-50
Identity = 129/322 (40.06%), Postives = 165/322 (51.24%), Query Frame = 0

Query: 4   ETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQ 63
           + LL  L T +FL   L P +S+  P  +  +G    K       G   +G+  VK Y  
Sbjct: 7   QELLVALATLYFLATSL-PSVSAHGP--YAWDGEATYK-FTTYHPGQNYKGLSNVKNYFH 66

Query: 64  HFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFA 123
           H GY+ N     +  FDD+ FD  L SAIKTYQ NYNL  TG  +  TL  +  PRCG  
Sbjct: 67  HLGYIPN-----APHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVP 126

Query: 124 DIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFL--PGTPS 183
           DI+    +  S              F  +S Y FF   P+W A  + LTY F   P    
Sbjct: 127 DIIINTNKTTS--------------FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDD 186

Query: 184 KAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAP 243
              + I RAF  W    +  F +T+ Y+ A+IKI F + +HGD + FDG  G++ H FAP
Sbjct: 187 TFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAP 246

Query: 244 RDGRLHFDAAESWAVGA------IADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIME 303
            DGR HFDA E W          +  +FDLE++A+HEIGHLLGL HSS  RAIM+PSI  
Sbjct: 247 TDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPP 304

Query: 304 GSTK-GLDADDIAGINALYNAN 317
            + K  L  DDI GI  LY  N
Sbjct: 307 RTRKVNLAQDDIDGIRKLYGIN 304

BLAST of CsaV3_5G037580 vs. TrEMBL
Match: tr|A0A0A0KU08|A0A0A0KU08_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 1.0e-181
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA
Sbjct: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG
Sbjct: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of CsaV3_5G037580 vs. TrEMBL
Match: tr|A0A1S3C9I6|A0A1S3C9I6_CUCME (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 3.4e-161
Identity = 277/316 (87.66%), Postives = 294/316 (93.04%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FETLLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNN       SFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNXXXXXXXSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASK HLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVA +GRAFKTWGA+THFNFS+ S+YKKADIKISF +GDHGDGHSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of CsaV3_5G037580 vs. TrEMBL
Match: tr|A0A0A0KS47|A0A0A0KS47_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.9e-124
Identity = 221/316 (69.94%), Postives = 257/316 (81.33%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M F+ L  F T + FL+I LFPLISSS PK    +GLVFLKNL GCKKGDKVEGIHQ+K+
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKP--VDGLVFLKNLKGCKKGDKVEGIHQMKK 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNN V   S+S  DDEFD +LE A+KTYQ+NYNLK TGT++  TL  MS+PRC
Sbjct: 61  YLQHFGYLNNDVHIRSES-SDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI++GKT MKSGK+ VNQHRKI+GHFH VSH+AFF+GN +WP SKSHLTYGFLPGTP
Sbjct: 121 GVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           S+A++ + RAF TW ANTHF+FSQ S+Y+ ADIKISFE GDHGDG  FD V GV+AH FA
Sbjct: 181 SEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E WA GA+  S+D+ET+ALHEIGHLLGL+HSS+E AIMWPSIM G+TKG
Sbjct: 241 PTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKG 300

Query: 301 LDADDIAGINALYNAN 317
           L ADDI GI  LY  +
Sbjct: 301 LHADDIEGIKVLYTTS 313

BLAST of CsaV3_5G037580 vs. TrEMBL
Match: tr|A0A1S3C925|A0A1S3C925_CUCME (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498039 PE=3 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.6e-118
Identity = 201/282 (71.28%), Postives = 239/282 (84.75%), Query Frame = 0

Query: 35  NGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKT 94
           +GLVFLKNLNGCKKGDKVEGIHQ+K+YLQHFGY+NN V  +SKS  DDEFD +LE A+KT
Sbjct: 2   DGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYMNNDVHIHSKS-SDDEFDEILEFAVKT 61

Query: 95  YQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSH 154
           YQ+NYNLKATGT++  T+  MS+PRCG ADI++G+T MKSGK++VN+HRKI GHFH VSH
Sbjct: 62  YQINYNLKATGTLDNSTISQMSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSH 121

Query: 155 YAFFDGNPKWPASKSHLTYGFLPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIK 214
           +AFF+GN KWP SKSHLTYGFLPGTPS+A++ + RAF TW ANTHF+FSQ S Y+ ADIK
Sbjct: 122 FAFFEGNLKWPNSKSHLTYGFLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIK 181

Query: 215 ISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIG 274
           ++FE GDHGDG  FD V GV+AH FAP DGRLHFDA E WA GA+ +S+D+ET+ALHEIG
Sbjct: 182 VTFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIG 241

Query: 275 HLLGLYHSSIERAIMWPSIMEGSTKGLDADDIAGINALYNAN 317
           HLLGL+HSS+E AIMWPSIM G+TKGL ADDI GI  LY  +
Sbjct: 242 HLLGLHHSSVEGAIMWPSIMGGTTKGLHADDIEGIKVLYTTS 282

BLAST of CsaV3_5G037580 vs. TrEMBL
Match: tr|A0A1S3C9Q9|A0A1S3C9Q9_CUCME (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498041 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.1e-106
Identity = 200/319 (62.70%), Postives = 235/319 (73.67%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNG-----LVFLKNLNGCKKGDKVEGI 60
           MA E L  F  T   L++  FPLISSS P    S+G      VFL  L+G +KGDKVEGI
Sbjct: 1   MALEELSFFALT--LLLLTFFPLISSSQPNHSHSHGKASSQFVFLNQLHGSQKGDKVEGI 60

Query: 61  HQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLM 120
           HQ+K+YL  FGYLN   Q +S++  DDEFD +LESAIKTYQ NYNLK TGT++A T+  M
Sbjct: 61  HQLKKYLHQFGYLNEDAQIHSEN-KDDEFDELLESAIKTYQKNYNLKVTGTLDAMTIAQM 120

Query: 121 SRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGF 180
            +PRCG ADI+ G T M+S KK       I GHFH VSH+AFFDGNPKWPA+KSHLTYGF
Sbjct: 121 LKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHRVSHFAFFDGNPKWPATKSHLTYGF 180

Query: 181 LPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVI 240
           LPGTP + V+ + RAF TW AN+HF FSQ    + +DI I FE+GDHGDG+ FDGV GV+
Sbjct: 181 LPGTPPETVSPVARAFATWAANSHFTFSQALDNQTSDITIGFESGDHGDGYPFDGVGGVV 240

Query: 241 AHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIME 300
           AH F+P DGR H DA ESWA G ++ SFDLET+ALHEIGHLLGL HSSIE AIMWP + E
Sbjct: 241 AHAFSPSDGRFHLDADESWANGVVSGSFDLETVALHEIGHLLGLQHSSIEGAIMWPQVPE 300

Query: 301 GSTKGLDADDIAGINALYN 315
           G++KGL ADDIAGI ALYN
Sbjct: 301 GASKGLHADDIAGIKALYN 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004142461.11.5e-181100.00PREDICTED: metalloendoproteinase 1-like [Cucumis sativus] >KGN52414.1 hypothetic... [more]
XP_008458907.15.1e-16187.66PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo][more]
XP_022938357.12.2e-13274.76metalloendoproteinase 2-MMP-like [Cucurbita moschata][more]
XP_023551500.11.4e-13174.92metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo][more]
XP_022993647.11.9e-12872.38metalloendoproteinase 2-MMP-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G70170.11.8e-6441.64matrix metalloproteinase[more]
AT1G24140.11.2e-6041.11Matrixin family protein[more]
AT1G59970.14.6e-5537.92Matrixin family protein[more]
AT4G16640.14.3e-5343.06Matrixin family protein[more]
AT2G45040.12.8e-4439.42Matrixin family protein[more]
Match NameE-valueIdentityDescription
sp|O04529|2MMP_ARATH3.3e-6341.64Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
sp|Q5XF51|3MMP_ARATH2.2e-5941.11Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
sp|Q9ZUJ5|5MMP_ARATH8.2e-5437.92Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
sp|O23507|1MMP_ARATH7.7e-5243.06Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
sp|P29136|MEP1_SOYBN3.2e-5040.06Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
tr|A0A0A0KU08|A0A0A0KU08_CUCSA1.0e-181100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G632130 PE=3 SV=1[more]
tr|A0A1S3C9I6|A0A1S3C9I6_CUCME3.4e-16187.66metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV... [more]
tr|A0A0A0KS47|A0A0A0KS47_CUCSA1.9e-12469.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1[more]
tr|A0A1S3C925|A0A1S3C925_CUCME1.6e-11871.28metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498039 PE=3 SV... [more]
tr|A0A1S3C9Q9|A0A1S3C9Q9_CUCME1.1e-10662.70metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498041 PE=3 SV... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0004222metalloendopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
Vocabulary: INTERPRO
TermDefinition
IPR036365PGBD-like_sf
IPR033739M10A_MMP
IPR002477Peptidoglycan-bd-like
IPR024079MetalloPept_cat_dom_sf
IPR001818Pept_M10_metallopeptidase
IPR006026Peptidase_Metallo
IPR021190Pept_M10A
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G037580.1CsaV3_5G037580.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 187..202
score: 36.59
coord: 268..293
score: 52.11
coord: 301..314
score: 52.7
coord: 211..239
score: 53.2
coord: 115..128
score: 43.64
IPR021190Peptidase M10APIRSFPIRSF001191MMP_stromelysincoord: 26..316
e-value: 2.6E-55
score: 185.9
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 160..315
e-value: 8.7E-31
score: 118.3
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 164..313
e-value: 5.8E-42
score: 143.1
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3DG3DSA:3.40.390.10coord: 42..316
e-value: 1.3E-72
score: 246.2
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 54..115
e-value: 3.3E-10
score: 39.9
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 7..315
NoneNo IPR availablePANTHERPTHR10201:SF213METALLOENDOPROTEINASE 2-MMP-RELATEDcoord: 7..315
NoneNo IPR availableSUPERFAMILYSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 156..316
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 163..313
e-value: 1.55972E-52
score: 171.233
IPR036365PGBD-like superfamilySUPERFAMILYSSF47090PGBD-likecoord: 46..125

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CsaV3_5G037580CsGy5G027840Cucumber (Gy14) v2cgybcucB232
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsaV3_5G037580Cucumber (Chinese Long) v3cuccucB041
CsaV3_5G037580Silver-seed gourdcarcucB0296
CsaV3_5G037580Silver-seed gourdcarcucB0725
CsaV3_5G037580Cucumber (Gy14) v2cgybcucB043
CsaV3_5G037580Cucumber (Gy14) v1cgycucB113
CsaV3_5G037580Cucumber (Gy14) v1cgycucB244
CsaV3_5G037580Cucurbita maxima (Rimu)cmacucB0262
CsaV3_5G037580Cucurbita maxima (Rimu)cmacucB0353
CsaV3_5G037580Cucurbita maxima (Rimu)cmacucB0503
CsaV3_5G037580Cucurbita maxima (Rimu)cmacucB0860
CsaV3_5G037580Cucurbita moschata (Rifu)cmocucB0250
CsaV3_5G037580Cucurbita moschata (Rifu)cmocucB0348
CsaV3_5G037580Cucurbita moschata (Rifu)cmocucB0846
CsaV3_5G037580Cucurbita pepo (Zucchini)cpecucB0210
CsaV3_5G037580Cucurbita pepo (Zucchini)cpecucB0517
CsaV3_5G037580Wild cucumber (PI 183967)cpicucB049
CsaV3_5G037580Wild cucumber (PI 183967)cpicucB284
CsaV3_5G037580Bottle gourd (USVL1VR-Ls)cuclsiB371
CsaV3_5G037580Bottle gourd (USVL1VR-Ls)cuclsiB375
CsaV3_5G037580Melon (DHL92) v3.5.1cucmeB358
CsaV3_5G037580Melon (DHL92) v3.5.1cucmeB394
CsaV3_5G037580Melon (DHL92) v3.6.1cucmedB350
CsaV3_5G037580Melon (DHL92) v3.6.1cucmedB389
CsaV3_5G037580Watermelon (Charleston Gray)cucwcgB363
CsaV3_5G037580Watermelon (Charleston Gray)cucwcgB366
CsaV3_5G037580Watermelon (Charleston Gray)cucwcgB391
CsaV3_5G037580Watermelon (97103) v1cucwmB388
CsaV3_5G037580Watermelon (97103) v1cucwmB447
CsaV3_5G037580Watermelon (97103) v2cucwmbB358
CsaV3_5G037580Watermelon (97103) v2cucwmbB425
CsaV3_5G037580Wax gourdcucwgoB482
CsaV3_5G037580Wax gourdcucwgoB512