CsaV3_5G024290 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_5G024290 vs. NCBI nr
Match: XP_004140357.1 (PREDICTED: nigrin b [Cucumis sativus] >KGN51070.1 Galactose-binding type-2 ribosome-inactivating protein [Cucumis sativus])

HSP 1 Score: 1068.5 bits (2762), Expect = 7.1e-309
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0

Query: 1   MRVLLAFIVTLSIATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDR 60
           MRVLLAFIVTLSIATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDR
Sbjct: 1   MRVLLAFIVTLSIATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDR 60

Query: 61  FHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNF 120
           FHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNF
Sbjct: 61  FHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNF 120

Query: 121 DNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFI 180
           DNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFI
Sbjct: 121 DNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFI 180

Query: 181 EQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEV 240
           EQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEV
Sbjct: 181 EQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEV 240

Query: 241 DSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVA 300
           DSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVA
Sbjct: 241 DSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVA 300

Query: 301 RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATW 360
           RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATW
Sbjct: 301 RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATW 360

Query: 361 DVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQM 420
           DVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQM
Sbjct: 361 DVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQM 420

Query: 421 CLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCN 480
           CLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCN
Sbjct: 421 CLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCN 480

Query: 481 GLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY 535
           GLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY
Sbjct: 481 GLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY 534

BLAST of CsaV3_5G024290 vs. NCBI nr
Match: XP_008463192.1 (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 989.2 bits (2556), Expect = 5.5e-285
Identity = 492/534 (92.13%), Postives = 509/534 (95.32%), Query Frame = 0

Query: 1   MRVLLAFIVTLSIATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDR 60
           MRVLLAF+VTLS ATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVL HSLSNSDR
Sbjct: 1   MRVLLAFVVTLSFATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLQHSLSNSDR 60

Query: 61  FHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNF 120
           FHLID GNEPGDTIT AVDAQDMSVVAYLAG++DSYFFSNAPKFAFDILFP TNQNLLNF
Sbjct: 61  FHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPNTNQNLLNF 120

Query: 121 DNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFI 180
           D+SFKSIE AANTTR+ATPLG K SNAAIANLFHYD VLAPVSFLIVFQM+FESAKF FI
Sbjct: 121 DSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIFESAKFNFI 180

Query: 181 EQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEV 240
           EQRIVNSI  GEAFTPDLAMLSLEDNWSELSL+IQASNSLQGLFGSSVTLYNSKNEA+EV
Sbjct: 181 EQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYNSKNEAIEV 240

Query: 241 DSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVA 300
           DSIYYPLILSNLALQLYHCNIQDYIKMPTATV DHQNPRCYVKERTVRISG+DGLC DVA
Sbjct: 241 DSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGRDGLCVDVA 300

Query: 301 RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATW 360
           RDGS VISSPCGQQANQ+WTF+RDHTIRSSDKCLIP KSKANP AVI +CNKVSQ+DATW
Sbjct: 301 RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKANPFAVILDCNKVSQQDATW 360

Query: 361 DVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQM 420
           DVSISGTIMNPAYDLVLTS NGINDNSLSMKKNKYCGNQGWRVGN+VEP+I SIIGVKQM
Sbjct: 361 DVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVEPLIVSIIGVKQM 420

Query: 421 CLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCN 480
           CLEATEENTNIWLEECVKNKIEQSWAV+SDGSIRVNNDHSLC+TASSIE KQRIVI KCN
Sbjct: 421 CLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSIEPKQRIVIGKCN 480

Query: 481 GLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY 535
           GLASQRWVL ADGTISTPK EGLVMDVAQSNVDLKEIVLYPRSDLVSQ WVALY
Sbjct: 481 GLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQQWVALY 534

BLAST of CsaV3_5G024290 vs. NCBI nr
Match: U3KRF8.2 (RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalpha chain; Contains: RecName: Full=Seed lectin Abeta chain; Contains: RecName: Full=Seed lectin B chain)

HSP 1 Score: 514.6 bits (1324), Expect = 4.0e-142
Identity = 259/463 (55.94%), Postives = 340/463 (73.43%), Query Frame = 0

Query: 27  YQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVV 86
           Y+++I  +R++ GS+T++LYGIPVL HSLSNS+RF+L+   +   ++IT A+D +DM  V
Sbjct: 16  YKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQDESITLAIDVEDMVAV 75

Query: 87  AYL-AGDNDSYFFSNAPKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGLKPS 146
           AY  AG ++SYFF NAP+ AF  LF  T+QN+LNFDN+FKS+E AA TTR+   LG+ P 
Sbjct: 76  AYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAGTTRQTIVLGVDPL 135

Query: 147 NAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFIEQRIVNSITNGEAFTPDLAMLSLED 206
           + AI+NLF+ DP L P+SFL++ QMV E++KF+FIEQ +  S  N + F PDLA++SLED
Sbjct: 136 DFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEKTFLPDLAIVSLED 195

Query: 207 NWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEVDSIYYPLILSNLALQLYHCNIQDYI 266
           NWSE+SL+IQAS SLQGLFGS V LYNS NE +EVDSIYYP+IL+N+ALQLYHC +    
Sbjct: 196 NWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANVALQLYHCQV---- 255

Query: 267 KMPTATVADHQNPRCYVKERTVRISGQDGLCADVA----RDGSHVISSPCGQQANQQWTF 326
                +  D++   C V+ RT RISG+D LC DVA     DGS +I  PCGQQ NQ+WTF
Sbjct: 256 -----STGDNE---CLVETRTTRISGRDALCVDVAGALTSDGSRLILYPCGQQVNQKWTF 315

Query: 327 HRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATWDVSISGTIMNPAY-DLVLTSK 386
           H D T+RS  KCL  N SK   L VI +C+K++ ED +WDVS+ GTIMNP Y DL LTS 
Sbjct: 316 HSDGTVRSLGKCLATNNSKFGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSN 375

Query: 387 NGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQMCLEATEENTNIWLEECVKNK 446
                 +L+M+ N Y  +QGWRVGNYV+PII SI+G+  MCLEAT+ NTN+WLEECV N+
Sbjct: 376 KATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECVPNQ 435

Query: 447 IEQSWAVFSDGSIRVNNDHSLCMTASS--IESKQRIVIAKCNG 482
            EQSWA++SDG+IRV+++  LC+TASS   ++ + I I  C+G
Sbjct: 436 REQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDG 466

BLAST of CsaV3_5G024290 vs. NCBI nr
Match: XP_022156703.1 (seed lectin-like [Momordica charantia] >BAH05018.1 galactose-binding type-2 ribosome-inactivating protein [Momordica charantia])

HSP 1 Score: 500.7 bits (1288), Expect = 6.0e-138
Identity = 264/548 (48.18%), Postives = 364/548 (66.42%), Query Frame = 0

Query: 1   MRVLLAFI-VTLSIATNAL-------SKRPVVDVYQSYIQTIRQKFG-SKTHKLYGIPVL 60
           MRVL  +I V LS+  N +             D Y+S+I+ +R++     ++   GIP+L
Sbjct: 3   MRVLAVYIVVALSLTINGIECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGIPIL 62

Query: 61  HHSLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFP 120
            HS+   +RF L+D  N   +TIT A++ +D    AY A D  SYFF NAP  A  ++F 
Sbjct: 63  KHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADR-SYFFQNAPPIASYVIFT 122

Query: 121 KTNQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMV 180
            TNQN++NF+N+F+SIEI   TTR  TPLG+    A+I +LF +D    P SFL++ QMV
Sbjct: 123 DTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLIQMV 182

Query: 181 FESAKFKFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLY 240
            E+AKFKFIEQ++++SI + E FTP LAMLSLE+NW++LSL++QAS SL G+FG SV+LY
Sbjct: 183 LEAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSVSLY 242

Query: 241 NSKNEAVEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISG 300
           NS +E + VDS+YYP++ +N+A QLY C       MPT T+ +  N +C  ++RT RISG
Sbjct: 243 NSMDEPIGVDSMYYPILTANMAFQLYQCPYGVIRMMPTITMPNQNNEQCSPQQRTTRISG 302

Query: 301 QDGLCADV----ARDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVI 360
           +DGLC DV      DGS VI  PCGQQ NQQWTF+ D+TIRS  KCL  +   +    VI
Sbjct: 303 RDGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSNVVI 362

Query: 361 QNCNKVSQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYV 420
            NC+ +  +D  W VS SGT+MN +  LVLT+    +  +L+ + N +   Q WR+GNYV
Sbjct: 363 TNCDYLRYDDG-WMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRIGNYV 422

Query: 421 EPIIASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASS 480
           EPI+ +IIG++ MCLEAT+ +TN+WLE CVKNK +Q WA++SD +IRVNN+ +LC+++S+
Sbjct: 423 EPIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCVSSST 482

Query: 481 IESKQRIVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVS 535
             S + IVI +C+G  +QRWV    GTIS P YE  VMDVAQ++V LK+IVL   +D  +
Sbjct: 483 DSSSKLIVIRRCDGSINQRWVFTPQGTISNPGYEA-VMDVAQNDVYLKKIVLSSATDKGN 542

BLAST of CsaV3_5G024290 vs. NCBI nr
Match: XP_011656527.1 (PREDICTED: nigrin b-like [Cucumis sativus] >KGN45978.1 hypothetical protein Csa_6G040650 [Cucumis sativus])

HSP 1 Score: 477.2 bits (1227), Expect = 7.1e-131
Identity = 257/543 (47.33%), Postives = 365/543 (67.22%), Query Frame = 0

Query: 3   VLLAF--IVTLSIATNALSKRP---VVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSN 62
           +++AF  + T SI  N LS      VVD Y+++I  IRQ+    T KLY IP+L  SL  
Sbjct: 8   IIIAFLSLATPSIDGNQLSLSSSDLVVDGYKNFINVIRQRLTENTLKLYDIPILESSLPP 67

Query: 63  SDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNL 122
           + RF  I+ GN+  +TI+ A+D  ++ VV Y   +N+SY F +APK A D++FP+T + +
Sbjct: 68  TQRFMNINIGNQNDETISLAIDRVNLGVVGY-RSNNNSYIFLDAPKAALDVVFPETCRVV 127

Query: 123 LNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKF 182
           L F++ ++SIE A+ TTR  T LGL+P N+AI+NLFHY     P SFL++ QMV E +KF
Sbjct: 128 LGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQMVLEGSKF 187

Query: 183 KFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEA 242
           KFIEQ ++ S+  G  F P LA++SLEDNW +LS +IQAS SLQGLFG ++ LY+S ++ 
Sbjct: 188 KFIEQSVIQSLKYGYNFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRLYDSNDKF 247

Query: 243 VEVDSIYYPLILSNLALQLYHCNIQ-DYIKMPTATVADHQNPRCYVKERTVRISGQDGLC 302
           ++VDSIYY +I +N+A QL+HCN+  ++I+MP+       +  C V+ RT  ISGQ+G C
Sbjct: 248 IDVDSIYYVIITTNIAFQLHHCNVSTNFIRMPSDV-----DDSCNVQTRTAMISGQNGFC 307

Query: 303 ADVAR----DGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNK 362
            D +R    +G+ +I   C  Q NQ+WTF  D TIR S+KCL    S+     V+ NC++
Sbjct: 308 VDSSRILDYNGNPIILYRCINQLNQEWTFLSDKTIRYSNKCLTFETSR---YVVLYNCSE 367

Query: 363 VSQE-DATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPII 422
           V ++ +  W+V+I GTI NP+  LVLT+    N + L ++ NK+  +QGWRVGNYV+PII
Sbjct: 368 VEEKGNIRWNVAIDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVGNYVKPII 427

Query: 423 ASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESK 482
            SIIG+++MCLEAT  NTN+WLE+CVKNK EQ WAV+SDGSIRVN   +LC+++SS  S 
Sbjct: 428 GSIIGMEEMCLEATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVSSSSNRSG 487

Query: 483 QRIVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWV 535
             ++I +C G ++QRW   A+GTI  P+ +  V+DV  S V  K I+LYP++ L +Q W 
Sbjct: 488 ALLIIDECKGTSNQRWNFLANGTILNPETK-KVVDVYGSMVSAKRIILYPKTGLANQQWT 540

BLAST of CsaV3_5G024290 vs. Swiss-Prot
Match: sp|U3KRF8|SGSL_TRIAN (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 514.6 bits (1324), Expect = 1.3e-144
Identity = 259/463 (55.94%), Postives = 340/463 (73.43%), Query Frame = 0

Query: 27  YQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVV 86
           Y+++I  +R++ GS+T++LYGIPVL HSLSNS+RF+L+   +   ++IT A+D +DM  V
Sbjct: 16  YKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQDESITLAIDVEDMVAV 75

Query: 87  AYL-AGDNDSYFFSNAPKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGLKPS 146
           AY  AG ++SYFF NAP+ AF  LF  T+QN+LNFDN+FKS+E AA TTR+   LG+ P 
Sbjct: 76  AYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAGTTRQTIVLGVDPL 135

Query: 147 NAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFIEQRIVNSITNGEAFTPDLAMLSLED 206
           + AI+NLF+ DP L P+SFL++ QMV E++KF+FIEQ +  S  N + F PDLA++SLED
Sbjct: 136 DFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEKTFLPDLAIVSLED 195

Query: 207 NWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEVDSIYYPLILSNLALQLYHCNIQDYI 266
           NWSE+SL+IQAS SLQGLFGS V LYNS NE +EVDSIYYP+IL+N+ALQLYHC +    
Sbjct: 196 NWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANVALQLYHCQV---- 255

Query: 267 KMPTATVADHQNPRCYVKERTVRISGQDGLCADVA----RDGSHVISSPCGQQANQQWTF 326
                +  D++   C V+ RT RISG+D LC DVA     DGS +I  PCGQQ NQ+WTF
Sbjct: 256 -----STGDNE---CLVETRTTRISGRDALCVDVAGALTSDGSRLILYPCGQQVNQKWTF 315

Query: 327 HRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATWDVSISGTIMNPAY-DLVLTSK 386
           H D T+RS  KCL  N SK   L VI +C+K++ ED +WDVS+ GTIMNP Y DL LTS 
Sbjct: 316 HSDGTVRSLGKCLATNNSKFGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSN 375

Query: 387 NGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQMCLEATEENTNIWLEECVKNK 446
                 +L+M+ N Y  +QGWRVGNYV+PII SI+G+  MCLEAT+ NTN+WLEECV N+
Sbjct: 376 KATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECVPNQ 435

Query: 447 IEQSWAVFSDGSIRVNNDHSLCMTASS--IESKQRIVIAKCNG 482
            EQSWA++SDG+IRV+++  LC+TASS   ++ + I I  C+G
Sbjct: 436 REQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDG 466

BLAST of CsaV3_5G024290 vs. Swiss-Prot
Match: sp|O22415|SNAIF_SAMNI (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.4e-98
Identity = 219/530 (41.32%), Postives = 299/530 (56.42%), Query Frame = 0

Query: 25  DVYQSYIQTIRQKFGSKTHKLYGIPVLH--HSLSNSDRFHLIDAGNEPGDTITFAVDAQD 84
           D Y  +++ +++K     H  + +PVL+    +S+S+RF L+   N  GDT+T A+D  +
Sbjct: 45  DTYGPFLRALQEKVILGNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVN 104

Query: 85  MSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGL 144
           + VVA+ + +  SYFFS +     D LF  T Q  LNF  ++ S+E      R   PLG 
Sbjct: 105 LYVVAF-SSNGRSYFFSGSTAVQRDNLFVDTTQEELNFTGNYISLERQVGFGRVYIPLGP 164

Query: 145 KPSNAAIANLFHY-----DPVLAPVSFLIVFQMVFESAKFKFIEQRIVNSITNGEAFTPD 204
           K    AI++L  Y     D        L+V QMV E+A+F++IE RI  SIT+   FTPD
Sbjct: 165 KSLAQAISSLRTYTLSAGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPD 224

Query: 205 LAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEVDSIYYPLILSNLALQLY 264
           L MLS+E+NWS +S EIQ +    G+F   V L + +N  +EV +      L+ +A+ LY
Sbjct: 225 LLMLSMENNWSSMSSEIQQAQP-GGIFPGVVQLRDERNNPIEVTNFRRLFELTYIAVLLY 284

Query: 265 HC----------NIQD--YIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVAR---- 324
            C          N  D   IKMP      ++   C V E T RISG DGLC DV      
Sbjct: 285 GCAPVTSNSYTNNAIDAQIIKMPVFRGGGYEKV-CSVVEVTRRISGWDGLCVDVRDGHYI 344

Query: 325 DGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATWD 384
           DG+ V   PCG + NQ WTF  D TIR   KCL  + S      +I +CN V  E   W 
Sbjct: 345 DGNTVQLGPCGNECNQLWTFRTDGTIRWLGKCLTTSSS-----VMIYDCNTVPPEATKWV 404

Query: 385 VSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQMC 444
           VS  GTI NP   LVLT+       +LS++ N +   QGW VG+ VEP++  I+G KQMC
Sbjct: 405 VSTDGTITNPRSGLVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMC 464

Query: 445 LEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCNG 504
           L    EN  +WLE+CV N++EQ WA++ DG+IRVN++ SLC+T+   E    IVI KC G
Sbjct: 465 LTENGENNFVWLEDCVLNRVEQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEG 524

Query: 505 LASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWV 532
             +QRWV   +GTIS P  + LVMDVAQSNV L++I+LYP +   +Q W+
Sbjct: 525 SGNQRWVFNTNGTISNPNAK-LVMDVAQSNVSLRKIILYPPTGNPNQQWI 564

BLAST of CsaV3_5G024290 vs. Swiss-Prot
Match: sp|P33183|NIGB_SAMNI (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 361.3 bits (926), Expect = 1.8e-98
Identity = 201/520 (38.65%), Postives = 304/520 (58.46%), Query Frame = 0

Query: 27  YQSYIQTIRQKFGSKTHKLYGIPVLHH--SLSNSDRFHLIDAGNEPGDTITFAVDAQDMS 86
           Y+ ++  +R+   + T+++ G+PVL     +    RF L+   N  G+T+T AVD  ++ 
Sbjct: 43  YRDFLSNLRKTVATGTYEVNGLPVLRRESEVQVKSRFVLVPLTNYNGNTVTLAVDVTNLY 102

Query: 87  VVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGLKP 146
           VVA+ +G+ +SYFF +A +     LF  T QN L+F  ++ ++E AANT RE+  LG  P
Sbjct: 103 VVAF-SGNANSYFFKDATEVQKSNLFVGTKQNTLSFTGNYDNLETAANTRRESIELGPSP 162

Query: 147 SNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFIEQRIVNSITNGEAFTPDLAMLSLE 206
            + AI +L+H D V    S L+V QMV E+A+F++IEQ +  S+    +FTP+  MLS+E
Sbjct: 163 LDGAITSLYHGDSVAR--SLLVVIQMVSEAARFRYIEQEVRRSLQQATSFTPNALMLSME 222

Query: 207 DNWSELSLEI-QASNSLQGLFGSSVTLYNSKNEAVEVDSIYYPLILSNLALQLYHCNI-- 266
           +NWS +SLEI QA N++   FG +V L N  +    VD+      ++ +A+ L+ C+   
Sbjct: 223 NNWSSMSLEIQQAGNNVSPFFG-TVQLLNYDHTHRLVDNFEELYKITGIAILLFRCSSPS 282

Query: 267 -QDYIKMPTATVAD----HQNPRCYVKERTVR-ISGQDGLCADVAR----DGSHVISSPC 326
             + I+MP     +    +    C ++    R I G+DGLC DV      DG+ +   PC
Sbjct: 283 NDNAIRMPLDLAGEDNKYNDGETCTLRTSFTRNIVGRDGLCVDVRNGYDTDGTPLQLWPC 342

Query: 327 GQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATWDVSISGTIMNP 386
           G Q NQ+WTF  D TIRS  KC+  N        VI NC+  ++    W+V I G+I+NP
Sbjct: 343 GTQRNQRWTFDSDDTIRSMGKCMTANGLNNGSNIVIFNCSTAAENAIKWEVPIDGSIINP 402

Query: 387 AYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQMCLEATEENTNI 446
           +  LV+T+    +   L ++ N Y  +QGW V N V+PI+ASI+G K+MCL++  EN  +
Sbjct: 403 SSGLVMTAPRAASRTILLLEDNIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGV 462

Query: 447 WLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCNGLASQRWVLKA 506
           W+E+C    ++Q WA++ D +IRVN+   LC+T +   SK  I+I KC GL SQRW   +
Sbjct: 463 WMEDCEATSLQQQWALYGDRTIRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNS 522

Query: 507 DGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWV 532
           DG I  PK    VMDV  SNV L+EI+++P +   +Q WV
Sbjct: 523 DGAIVNPKSRH-VMDVRASNVSLREIIIFPATGNPNQQWV 557

BLAST of CsaV3_5G024290 vs. Swiss-Prot
Match: sp|Q41358|SNAIB_SAMNI (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 4.1e-98
Identity = 215/530 (40.57%), Postives = 302/530 (56.98%), Query Frame = 0

Query: 25  DVYQSYIQTIRQKFGSKTHKLYGIPVLH--HSLSNSDRFHLIDAGNEPGDTITFAVDAQD 84
           D Y+ +++ +++K     H  + +PVL+    +S+S+RF L+   N  GDT+T A+D  +
Sbjct: 45  DTYEPFLRALQEKVILGNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVN 104

Query: 85  MSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGL 144
           + VVA+ + +  SYFFS +     D LF  T Q  LNF  ++ S+E      R   PLG 
Sbjct: 105 LYVVAF-SSNGKSYFFSGSTAVQRDNLFVDTTQEELNFTGNYTSLERQVGFGRVYIPLGP 164

Query: 145 KPSNAAIANLFHY-----DPVLAPVSFLIVFQMVFESAKFKFIEQRIVNSITNGEAFTPD 204
           K  + AI++L  Y     D        L+V QMV E+A+F++IE RI  SIT+   FTPD
Sbjct: 165 KSLDQAISSLRTYTLTAGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPD 224

Query: 205 LAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEVDSIYYPLILSNLALQLY 264
           L MLS+E+NWS +S EIQ +    G+F   V L + +N ++EV +      L+ +A+ LY
Sbjct: 225 LLMLSMENNWSSMSSEIQQAQP-GGIFAGVVQLRDERNNSIEVTNFRRLFELTYIAVLLY 284

Query: 265 HC----------NIQD--YIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVAR---- 324
            C          N  D   IKMP     +++   C V E T RISG DGLC DV      
Sbjct: 285 GCAPVTSSSYSNNAIDAQIIKMPVFRGGEYEKV-CSVVEVTRRISGWDGLCVDVRYGHYI 344

Query: 325 DGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATWD 384
           DG+ V   PCG + NQ WTF  D TIR   KCL  + S      +I +CN V  E   W 
Sbjct: 345 DGNPVQLRPCGNECNQLWTFRTDGTIRWLGKCLTASSS-----VMIYDCNTVPPEATKWV 404

Query: 385 VSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQMC 444
           VSI GTI NP   LVLT+       +LS++ N +   QGW VG+ VEP++  I+G KQMC
Sbjct: 405 VSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMC 464

Query: 445 LEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCNG 504
           L    EN  +WLE+CV N+++Q WA++ DG+IRVN++ SLC+T+   E    IVI KC G
Sbjct: 465 LRENGENNFVWLEDCVLNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEG 524

Query: 505 LASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWV 532
             +QRWV   +GTIS P  + L+MDVAQ +V L++I+LY  +   +Q W+
Sbjct: 525 SGNQRWVFNTNGTISNPNAK-LLMDVAQRDVSLRKIILYRPTGNPNQQWI 564

BLAST of CsaV3_5G024290 vs. Swiss-Prot
Match: sp|P93543|RIP1_SAMNI (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.3e-79
Identity = 186/474 (39.24%), Postives = 266/474 (56.12%), Query Frame = 0

Query: 27  YQSYIQTIRQKFGSKTHKLYGIPVLH--HSLSNSDRFHLIDAGNEPGDTITFAVDAQDMS 86
           Y+ ++Q +R K    +H+ + +PVL+    +S+SDRF L+   N     +T A+D     
Sbjct: 49  YRHFLQELRGKVILGSHRAFDLPVLNPESKVSDSDRFVLVRLTNPSRKKVTLAIDVVTFY 108

Query: 87  VVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGLKP 146
           VVA+   D  SYFFS + +   + LF  T Q  LNF   + S+E      R   PLG K 
Sbjct: 109 VVAFAQNDR-SYFFSGSSEVQRENLFVDTTQEDLNFKGDYTSLEHQVGFGRVYIPLGPKS 168

Query: 147 SNAAIANLFHY-----DPVLAPVSFLIVFQMVFESAKFKFIEQRIVNSITNGEAFTPDLA 206
              +I++L  Y     D      S L+V QMV E+A+F++I+ RI  SIT+ + FTPDL 
Sbjct: 169 LAQSISSLSTYKSSAGDNKRLARSLLVVIQMVSEAARFRYIQLRIQASITDAKEFTPDLL 228

Query: 207 MLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEVDSIYYPLILSNLALQLYHC 266
           MLS+E+ WS +S EIQ +    G F   V L + +N  ++V +      L+++A+ L+ C
Sbjct: 229 MLSMENKWSSMSSEIQQAQP-GGAFAQVVKLLDQRNHPIDVTNFRRLFQLTSVAVLLHGC 288

Query: 267 ----NIQDY-IKMPTATVADHQNPRCYVKERTVRISGQDGLCADV----ARDGSHVISSP 326
                +  Y IKMP     + +     V+E T RI G+DG CA+V     +DG+ V  S 
Sbjct: 289 PTVTKMPAYIIKMPVFNGGEDEERCSVVEEVTRRIGGRDGFCAEVKNGDEKDGTPVQLSS 348

Query: 327 CGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATWDVSISGTIMN 386
           CG+Q+NQQWTF  D TI+S  KCL  + S      +I NC  V  E   W VSI GTI N
Sbjct: 349 CGEQSNQQWTFSTDGTIQSLGKCLTTSSS-----VMIYNCKVVPPESTKWVVSIDGTITN 408

Query: 387 PAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQMCLEATEENTN 446
           P   LVLT+        +S++KN +   QGW VGN VEP++  I+G +QMCLE    N +
Sbjct: 409 PRSGLVLTAPKAAEGTLVSLEKNVHAARQGWIVGN-VEPLVTFIVGYEQMCLETNPGNND 468

Query: 447 IWLEEC-VK--NKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCNG 482
           + L +C VK  +K++Q WA++ DG+IRVNND SLC+T+    S + I+I KC G
Sbjct: 469 VSLGDCSVKSASKVDQKWALYGDGTIRVNNDRSLCVTSEGKSSNEPIIILKCLG 514

BLAST of CsaV3_5G024290 vs. TrEMBL
Match: tr|A0A0A0KN71|A0A0A0KN71_CUCSA (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 4.7e-309
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0

Query: 1   MRVLLAFIVTLSIATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDR 60
           MRVLLAFIVTLSIATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDR
Sbjct: 1   MRVLLAFIVTLSIATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDR 60

Query: 61  FHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNF 120
           FHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNF
Sbjct: 61  FHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNF 120

Query: 121 DNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFI 180
           DNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFI
Sbjct: 121 DNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFI 180

Query: 181 EQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEV 240
           EQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEV
Sbjct: 181 EQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEV 240

Query: 241 DSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVA 300
           DSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVA
Sbjct: 241 DSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVA 300

Query: 301 RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATW 360
           RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATW
Sbjct: 301 RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATW 360

Query: 361 DVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQM 420
           DVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQM
Sbjct: 361 DVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQM 420

Query: 421 CLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCN 480
           CLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCN
Sbjct: 421 CLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCN 480

Query: 481 GLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY 535
           GLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY
Sbjct: 481 GLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY 534

BLAST of CsaV3_5G024290 vs. TrEMBL
Match: tr|A0A1S3CIM5|A0A1S3CIM5_CUCME (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 3.7e-285
Identity = 492/534 (92.13%), Postives = 509/534 (95.32%), Query Frame = 0

Query: 1   MRVLLAFIVTLSIATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDR 60
           MRVLLAF+VTLS ATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVL HSLSNSDR
Sbjct: 1   MRVLLAFVVTLSFATNALSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLQHSLSNSDR 60

Query: 61  FHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNF 120
           FHLID GNEPGDTIT AVDAQDMSVVAYLAG++DSYFFSNAPKFAFDILFP TNQNLLNF
Sbjct: 61  FHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPNTNQNLLNF 120

Query: 121 DNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFI 180
           D+SFKSIE AANTTR+ATPLG K SNAAIANLFHYD VLAPVSFLIVFQM+FESAKF FI
Sbjct: 121 DSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIFESAKFNFI 180

Query: 181 EQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEV 240
           EQRIVNSI  GEAFTPDLAMLSLEDNWSELSL+IQASNSLQGLFGSSVTLYNSKNEA+EV
Sbjct: 181 EQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYNSKNEAIEV 240

Query: 241 DSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCADVA 300
           DSIYYPLILSNLALQLYHCNIQDYIKMPTATV DHQNPRCYVKERTVRISG+DGLC DVA
Sbjct: 241 DSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGRDGLCVDVA 300

Query: 301 RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATW 360
           RDGS VISSPCGQQANQ+WTF+RDHTIRSSDKCLIP KSKANP AVI +CNKVSQ+DATW
Sbjct: 301 RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKANPFAVILDCNKVSQQDATW 360

Query: 361 DVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQM 420
           DVSISGTIMNPAYDLVLTS NGINDNSLSMKKNKYCGNQGWRVGN+VEP+I SIIGVKQM
Sbjct: 361 DVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVEPLIVSIIGVKQM 420

Query: 421 CLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIAKCN 480
           CLEATEENTNIWLEECVKNKIEQSWAV+SDGSIRVNNDHSLC+TASSIE KQRIVI KCN
Sbjct: 421 CLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSIEPKQRIVIGKCN 480

Query: 481 GLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY 535
           GLASQRWVL ADGTISTPK EGLVMDVAQSNVDLKEIVLYPRSDLVSQ WVALY
Sbjct: 481 GLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQQWVALY 534

BLAST of CsaV3_5G024290 vs. TrEMBL
Match: tr|B7X8M2|B7X8M2_MOMCH (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 3.9e-138
Identity = 264/548 (48.18%), Postives = 364/548 (66.42%), Query Frame = 0

Query: 1   MRVLLAFI-VTLSIATNAL-------SKRPVVDVYQSYIQTIRQKFG-SKTHKLYGIPVL 60
           MRVL  +I V LS+  N +             D Y+S+I+ +R++     ++   GIP+L
Sbjct: 3   MRVLAVYIVVALSLTINGIECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGIPIL 62

Query: 61  HHSLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFP 120
            HS+   +RF L+D  N   +TIT A++ +D    AY A D  SYFF NAP  A  ++F 
Sbjct: 63  KHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADR-SYFFQNAPPIASYVIFT 122

Query: 121 KTNQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMV 180
            TNQN++NF+N+F+SIEI   TTR  TPLG+    A+I +LF +D    P SFL++ QMV
Sbjct: 123 DTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLIQMV 182

Query: 181 FESAKFKFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLY 240
            E+AKFKFIEQ++++SI + E FTP LAMLSLE+NW++LSL++QAS SL G+FG SV+LY
Sbjct: 183 LEAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSVSLY 242

Query: 241 NSKNEAVEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISG 300
           NS +E + VDS+YYP++ +N+A QLY C       MPT T+ +  N +C  ++RT RISG
Sbjct: 243 NSMDEPIGVDSMYYPILTANMAFQLYQCPYGVIRMMPTITMPNQNNEQCSPQQRTTRISG 302

Query: 301 QDGLCADV----ARDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVI 360
           +DGLC DV      DGS VI  PCGQQ NQQWTF+ D+TIRS  KCL  +   +    VI
Sbjct: 303 RDGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSNVVI 362

Query: 361 QNCNKVSQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYV 420
            NC+ +  +D  W VS SGT+MN +  LVLT+    +  +L+ + N +   Q WR+GNYV
Sbjct: 363 TNCDYLRYDDG-WMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRIGNYV 422

Query: 421 EPIIASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASS 480
           EPI+ +IIG++ MCLEAT+ +TN+WLE CVKNK +Q WA++SD +IRVNN+ +LC+++S+
Sbjct: 423 EPIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCVSSST 482

Query: 481 IESKQRIVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVS 535
             S + IVI +C+G  +QRWV    GTIS P YE  VMDVAQ++V LK+IVL   +D  +
Sbjct: 483 DSSSKLIVIRRCDGSINQRWVFTPQGTISNPGYEA-VMDVAQNDVYLKKIVLSSATDKGN 542

BLAST of CsaV3_5G024290 vs. TrEMBL
Match: tr|A0A0A0K979|A0A0A0K979_CUCSA (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.7e-131
Identity = 257/543 (47.33%), Postives = 365/543 (67.22%), Query Frame = 0

Query: 3   VLLAF--IVTLSIATNALSKRP---VVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSN 62
           +++AF  + T SI  N LS      VVD Y+++I  IRQ+    T KLY IP+L  SL  
Sbjct: 8   IIIAFLSLATPSIDGNQLSLSSSDLVVDGYKNFINVIRQRLTENTLKLYDIPILESSLPP 67

Query: 63  SDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNL 122
           + RF  I+ GN+  +TI+ A+D  ++ VV Y   +N+SY F +APK A D++FP+T + +
Sbjct: 68  TQRFMNINIGNQNDETISLAIDRVNLGVVGY-RSNNNSYIFLDAPKAALDVVFPETCRVV 127

Query: 123 LNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKF 182
           L F++ ++SIE A+ TTR  T LGL+P N+AI+NLFHY     P SFL++ QMV E +KF
Sbjct: 128 LGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQMVLEGSKF 187

Query: 183 KFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEA 242
           KFIEQ ++ S+  G  F P LA++SLEDNW +LS +IQAS SLQGLFG ++ LY+S ++ 
Sbjct: 188 KFIEQSVIQSLKYGYNFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRLYDSNDKF 247

Query: 243 VEVDSIYYPLILSNLALQLYHCNIQ-DYIKMPTATVADHQNPRCYVKERTVRISGQDGLC 302
           ++VDSIYY +I +N+A QL+HCN+  ++I+MP+       +  C V+ RT  ISGQ+G C
Sbjct: 248 IDVDSIYYVIITTNIAFQLHHCNVSTNFIRMPSDV-----DDSCNVQTRTAMISGQNGFC 307

Query: 303 ADVAR----DGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNK 362
            D +R    +G+ +I   C  Q NQ+WTF  D TIR S+KCL    S+     V+ NC++
Sbjct: 308 VDSSRILDYNGNPIILYRCINQLNQEWTFLSDKTIRYSNKCLTFETSR---YVVLYNCSE 367

Query: 363 VSQE-DATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPII 422
           V ++ +  W+V+I GTI NP+  LVLT+    N + L ++ NK+  +QGWRVGNYV+PII
Sbjct: 368 VEEKGNIRWNVAIDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVGNYVKPII 427

Query: 423 ASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESK 482
            SIIG+++MCLEAT  NTN+WLE+CVKNK EQ WAV+SDGSIRVN   +LC+++SS  S 
Sbjct: 428 GSIIGMEEMCLEATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVSSSSNRSG 487

Query: 483 QRIVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWV 535
             ++I +C G ++QRW   A+GTI  P+ +  V+DV  S V  K I+LYP++ L +Q W 
Sbjct: 488 ALLIIDECKGTSNQRWNFLANGTILNPETK-KVVDVYGSMVSAKRIILYPKTGLANQQWT 540

BLAST of CsaV3_5G024290 vs. TrEMBL
Match: tr|A0A1S3BDI7|A0A1S3BDI7_CUCME (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103488711 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.0e-130
Identity = 253/541 (46.77%), Postives = 361/541 (66.73%), Query Frame = 0

Query: 8   IVTLSIATNAL--------SKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSD 67
           I  LS+AT  +        S   +VD Y+++I  IRQ+    T KLY IP+L  SL  + 
Sbjct: 9   IAFLSLATRRIDGNQHSLSSSDLLVDGYKNFINVIRQRLTENTSKLYDIPILKSSLPPTQ 68

Query: 68  RFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLN 127
           RF  I+  N+  +TI+ A+D  ++ V+ Y   +N SY F +AP  AFDI+FPKT + LL 
Sbjct: 69  RFMTINIVNQNDETISLAIDKVNLGVLGY-RSNNTSYIFLDAPLVAFDIVFPKTCRVLLG 128

Query: 128 FDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKF 187
           F+++++SIE A+ TTR  T LGL+P N+AI+NLFHY     P SFL++ QMV E +KFKF
Sbjct: 129 FNSNYESIEKASGTTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMVLEGSKFKF 188

Query: 188 IEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVE 247
           IE+ ++ S+  G  F P LA++SLEDNW +LS ++QAS SLQGLFG ++ LY+S N+ +E
Sbjct: 189 IERSVIQSLKYGYNFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLYDSNNKVIE 248

Query: 248 VDSIYYPLILSNLALQLYHCNIQ-DYIKMPTATVADHQNPRCYVKERTVRISGQDGLCAD 307
           VDSIYY +I +N+A QL+HCNI  ++I+MP + V D     C V+ RT  I+GQ+G C D
Sbjct: 249 VDSIYYAIITTNIAFQLHHCNISTNFIRMP-SDVGD----SCNVQTRTATITGQNGFCVD 308

Query: 308 VA----RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVS 367
            +    +D + +I   CG Q+NQ+WTF  D TIR S+KCL  + S+     V+ NC++V 
Sbjct: 309 ASNGLHQDDNSIILYRCGYQSNQEWTFLSDKTIRYSNKCLTFDTSR---FVVLYNCSEVE 368

Query: 368 QE-DATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIAS 427
           ++ +  W+V+I GTI NP+  LVLT+    N + L ++ NK+  +QGWRVGNYVEP+  S
Sbjct: 369 KKSNIRWNVAIDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWRVGNYVEPLTGS 428

Query: 428 IIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQR 487
           IIG+++MCLEAT  NTN+WLE+CVKNK EQ WAV+ DGSIRVN+  +LC+++SS      
Sbjct: 429 IIGLEEMCLEATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRILAL 488

Query: 488 IVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVAL 535
           I+I +CNG ++QRW   A+G I  P+ + +VMDV +  V  K+I+LY ++   +Q W   
Sbjct: 489 IIIEECNGTSNQRWNFLANGAILNPETK-MVMDVYRFMVSRKKIILYRKTGERNQEWALF 539