CsaV3_5G020280 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_5G020280 vs. NCBI nr
Match: XP_004148908.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 AMP dependent ligase [Cucumis sativus])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGPPQKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of CsaV3_5G020280 vs. NCBI nr
Match: XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 527/557 (94.61%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of CsaV3_5G020280 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata])

HSP 1 Score: 938.7 bits (2425), Expect = 8.9e-270
Identity = 449/557 (80.61%), Postives = 497/557 (89.23%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNT AMYELHFAVPMAGA+LNTINLRLD RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SC LI DALALFPP+MKRP L+LI D+ A +DSS  S V  R +FVDSYE MV+ GD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+HVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+KAM+ 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of CsaV3_5G020280 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 906.7 bits (2342), Expect = 3.8e-260
Identity = 438/561 (78.07%), Postives = 485/561 (86.45%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNTPAMYELHFAVPMAGA+LNTINLRLD+RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMK----RPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSG 180
           SC LI DALALFPP+MK                          S R +FVDSYE MV+ G
Sbjct: 121 SCGLISDALALFPPEMKXXXXXXXXXXXXXXXXXXXXXXXXXXSNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           D  FQWIRP+SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLKARQGVR LA+   DVL PET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+FM E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 FQKFVLRQKAKSLGPPQKSRL 558
            QKFVLR+ AKS+GP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of CsaV3_5G020280 vs. NCBI nr
Match: XP_022922128.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata])

HSP 1 Score: 902.5 bits (2331), Expect = 7.1e-259
Identity = 433/555 (78.02%), Postives = 488/555 (87.93%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           ME+LKPRPANS PLTP+GF+ERAA IYGDS SIIY  TTYTWS THRRCLQLASS+SS G
Sbjct: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV +PNTP MYELHFAVPM GAILNTIN RLD RTISILL HSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
            C LI ++L LFPP++ RP+LVLI DD   ++SS  S V I  NFV SYE M++ GDS+F
Sbjct: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESS--SSVLIEGNFVGSYEEMIRKGDSEF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG F V LD +LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSF WGIAAVGGTN+CIRKFDS L++SLIRRHHVTHLCGAPVVLNMLTNSP+  PL
Sbjct: 241 HANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           D PVKILTAGAP PA++LLR ESLGF VTHGYGLTE+ GT++CCSWK+EWNQLPA+E+AR
Sbjct: 301 DWPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LK+RQGVR +A  AV+V+D ET KSV RDG SIGEIV+R  SLMLGY KD EAT K+M+E
Sbjct: 361 LKSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY NP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SL+EGL++KPTEEEIIE+CK KLP+FMVPKTV+FMTE+PKT+TGK QK+
Sbjct: 481 EFWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKY 540

Query: 541 VLRQKAKSLGPPQKS 556
           +LR+ A S+ P ++S
Sbjct: 541 LLREMAMSMDPTKRS 553

BLAST of CsaV3_5G020280 vs. TAIR10
Match: AT5G16370.1 (acyl activating enzyme 5)

HSP 1 Score: 751.5 bits (1939), Expect = 3.7e-217
Identity = 359/558 (64.34%), Postives = 454/558 (81.36%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV I D   +++    + V+ R  F  +Y++++  GD  
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA+VLLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           D FWGETPCAF+SLK GL+++PTE E+IEYC+ K+PK+MVPKTV F+ E+PKT+TGK  K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQKAKSLGPPQKSRL 558
           FVLR+ AK +G  + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of CsaV3_5G020280 vs. TAIR10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 748.0 bits (1930), Expect = 4.1e-216
Identity = 357/551 (64.79%), Postives = 449/551 (81.49%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV+I D   E++  +  V  +   F  +Y+++++ GD  
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIAD--KEEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA+VLLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           DEFWGETPCAF+SLK G S KPTEEE++EYC+ K+PK+MVPKTV FM E+PK++TGK  K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQKAKSLG 551
           FVLR  AK +G
Sbjct: 541 FVLRDIAKKMG 545

BLAST of CsaV3_5G020280 vs. TAIR10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 696.4 bits (1796), Expect = 1.4e-200
Identity = 339/553 (61.30%), Postives = 426/553 (77.03%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV + NTP MYEL F+VPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 PSCALIFDALALF--PPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGD 180
               L  +A+ +   P               AE        V+ R  F   Y +++  G+
Sbjct: 121 FYSDLAVEAITMLLNPXXXXXXXXXXXXXXXAE--------VTERSKFCYLYSDLITRGN 180

Query: 181 SKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTL 240
             F+WIRP SEWDPIV+NYTSGTTS+PKGVV+CHRG+F + LDS+ +W VPK  VYLWTL
Sbjct: 181 PDFKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTL 240

Query: 241 PMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD- 300
           P+FH NGW++PWGIAAVGGTNVC+RK  +  I+ LIR H VTH+ GAP+VL +L+ S + 
Sbjct: 241 PIFHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQES 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           + PL  PV  LTAG+ PPA+VLLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA 
Sbjct: 301 DQPLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPAS 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           +QA+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+   T  
Sbjct: 361 DQAQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQN 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           +  ++GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNEAAVV
Sbjct: 421 SF-KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLK GL+RKPT++EIIEYCK K+P++M PKTV F+ E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGK 540

Query: 541 FQKFVLRQKAKSL 550
             K +L++ AK++
Sbjct: 541 IIKSLLKEIAKNM 544

BLAST of CsaV3_5G020280 vs. TAIR10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 689.9 bits (1779), Expect = 1.3e-198
Identity = 330/555 (59.46%), Postives = 428/555 (77.12%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++  +T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI RG VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSL-PSVVSIRDNFVDSYENMVKSG 180
           D  S +++ +A++ F    ++P LVL+ DD  ++D SL PS  S   +F+D+Y+ +++ G
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAAS---DFLDTYQGVMERG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           DS+F+WIRP +EW P++LNYTSGTTS+PKGVV  HR +F + + S+L+W  P + VYLWT
Sbjct: 181 DSRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGW + WG AAVG TNVC R+ D+  I+ LI +HHVTH+C AP+VLNMLTN P 
Sbjct: 241 LPMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
             PL  PV+++TAGAPPPA+++ R E+LGF V HGYGLTE+ G ++ C+WK+EW+ L  +
Sbjct: 301 RKPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPL 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLK+RQGVR +    VDV DP T KSV+ DG S+GEIV++G S+MLGY KD E T+ 
Sbjct: 361 ERARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAA 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
            M EDGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NP+V EAAVV
Sbjct: 421 CMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRK--PTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTAT 540
           A+PD+ WGETPCAF+SLK   +     TE EI E+CK +LPK+MVP+ VIF  E+PKT+T
Sbjct: 481 AKPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKFQKFVLRQKAKSL 550
           GK QKF+LRQ AKSL
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of CsaV3_5G020280 vs. TAIR10
Match: AT1G21530.2 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 682.6 bits (1760), Expect = 2.1e-196
Identity = 328/556 (58.99%), Postives = 429/556 (77.16%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYN-STTYTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++  +T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI++G VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSES+L+FV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGD 180
           D  S +L+ +A++LF    ++P LVL+ DD  E DSS  S      +F+D+YE +++ G+
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLL-DDDQENDSSSAS------DFLDTYEEIMERGN 180

Query: 181 SKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTL 240
           S+F+WIRP +EW P+VLNYTSGTTS+PKGVV  HR +F + + S+L+W VP + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTL 240

Query: 241 PMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFH NGW + WG AAVG TN+C R+ D+  I++LI +H+VTH+C AP+VLNML N P +
Sbjct: 241 PMFHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLS 300

Query: 301 LPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIE 360
            PL  PV+++T+GAPPPA+++ R ESLGF V+H YGLTE++G ++ C+WK +W+ L  +E
Sbjct: 301 TPLKNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKA 420
           +ARLK+RQGVR L    VDV D +T KSVK DG S+GEIV RG S+MLGY KD + T+  
Sbjct: 361 RARLKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAAC 420

Query: 421 MSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVA 480
           M EDGWF++GD+GV+H DGY+EIKDRSKDVII GGEN+SS E+E+VLY NP+V EAAVVA
Sbjct: 421 MREDGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFISLK---EGLSRKP-TEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTA 540
           +PD+ WGETPCAF+SLK    G    P TE EI E+CK KLPK+MVP+ VIF  E+PKT+
Sbjct: 481 KPDKMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTS 540

Query: 541 TGKFQKFVLRQKAKSL 550
           TGK QKF+LRQ AK+L
Sbjct: 541 TGKIQKFLLRQMAKTL 548

BLAST of CsaV3_5G020280 vs. Swiss-Prot
Match: sp|Q9FFE6|AAE5_ARATH (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 6.6e-216
Identity = 359/558 (64.34%), Postives = 454/558 (81.36%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV I D   +++    + V+ R  F  +Y++++  GD  
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA+VLLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           D FWGETPCAF+SLK GL+++PTE E+IEYC+ K+PK+MVPKTV F+ E+PKT+TGK  K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQKAKSLGPPQKSRL 558
           FVLR+ AK +G  + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of CsaV3_5G020280 vs. Swiss-Prot
Match: sp|Q9FFE9|AAE6_ARATH (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 7.3e-215
Identity = 357/551 (64.79%), Postives = 449/551 (81.49%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV+I D   E++  +  V  +   F  +Y+++++ GD  
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIAD--KEEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA+VLLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           DEFWGETPCAF+SLK G S KPTEEE++EYC+ K+PK+MVPKTV FM E+PK++TGK  K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQKAKSLG 551
           FVLR  AK +G
Sbjct: 541 FVLRDIAKKMG 545

BLAST of CsaV3_5G020280 vs. Swiss-Prot
Match: sp|Q9LQS1|AAE8_ARATH (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.5e-199
Identity = 339/553 (61.30%), Postives = 426/553 (77.03%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV + NTP MYEL F+VPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 PSCALIFDALALF--PPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGD 180
               L  +A+ +   P               AE        V+ R  F   Y +++  G+
Sbjct: 121 FYSDLAVEAITMLLNPXXXXXXXXXXXXXXXAE--------VTERSKFCYLYSDLITRGN 180

Query: 181 SKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTL 240
             F+WIRP SEWDPIV+NYTSGTTS+PKGVV+CHRG+F + LDS+ +W VPK  VYLWTL
Sbjct: 181 PDFKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTL 240

Query: 241 PMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD- 300
           P+FH NGW++PWGIAAVGGTNVC+RK  +  I+ LIR H VTH+ GAP+VL +L+ S + 
Sbjct: 241 PIFHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQES 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           + PL  PV  LTAG+ PPA+VLLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA 
Sbjct: 301 DQPLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPAS 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           +QA+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+   T  
Sbjct: 361 DQAQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQN 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           +  ++GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNEAAVV
Sbjct: 421 SF-KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLK GL+RKPT++EIIEYCK K+P++M PKTV F+ E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGK 540

Query: 541 FQKFVLRQKAKSL 550
             K +L++ AK++
Sbjct: 541 IIKSLLKEIAKNM 544

BLAST of CsaV3_5G020280 vs. Swiss-Prot
Match: sp|Q9LPK6|AEE9_ARATH (Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana OX=3702 GN=AEE9 PE=2 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.4e-197
Identity = 330/555 (59.46%), Postives = 428/555 (77.12%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++  +T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI RG VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSL-PSVVSIRDNFVDSYENMVKSG 180
           D  S +++ +A++ F    ++P LVL+ DD  ++D SL PS  S   +F+D+Y+ +++ G
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAAS---DFLDTYQGVMERG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           DS+F+WIRP +EW P++LNYTSGTTS+PKGVV  HR +F + + S+L+W  P + VYLWT
Sbjct: 181 DSRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGW + WG AAVG TNVC R+ D+  I+ LI +HHVTH+C AP+VLNMLTN P 
Sbjct: 241 LPMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
             PL  PV+++TAGAPPPA+++ R E+LGF V HGYGLTE+ G ++ C+WK+EW+ L  +
Sbjct: 301 RKPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPL 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLK+RQGVR +    VDV DP T KSV+ DG S+GEIV++G S+MLGY KD E T+ 
Sbjct: 361 ERARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAA 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
            M EDGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NP+V EAAVV
Sbjct: 421 CMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRK--PTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTAT 540
           A+PD+ WGETPCAF+SLK   +     TE EI E+CK +LPK+MVP+ VIF  E+PKT+T
Sbjct: 481 AKPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKFQKFVLRQKAKSL 550
           GK QKF+LRQ AKSL
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of CsaV3_5G020280 vs. Swiss-Prot
Match: sp|Q9LPK7|AEE10_ARATH (Probable acyl-activating enzyme 10 OS=Arabidopsis thaliana OX=3702 GN=AEE10 PE=2 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 3.8e-195
Identity = 328/556 (58.99%), Postives = 429/556 (77.16%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYN-STTYTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++  +T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI++G VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSES+L+FV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGD 180
           D  S +L+ +A++LF    ++P LVL+ DD  E DSS  S      +F+D+YE +++ G+
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLL-DDDQENDSSSAS------DFLDTYEEIMERGN 180

Query: 181 SKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTL 240
           S+F+WIRP +EW P+VLNYTSGTTS+PKGVV  HR +F + + S+L+W VP + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTL 240

Query: 241 PMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFH NGW + WG AAVG TN+C R+ D+  I++LI +H+VTH+C AP+VLNML N P +
Sbjct: 241 PMFHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLS 300

Query: 301 LPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIE 360
            PL  PV+++T+GAPPPA+++ R ESLGF V+H YGLTE++G ++ C+WK +W+ L  +E
Sbjct: 301 TPLKNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKA 420
           +ARLK+RQGVR L    VDV D +T KSVK DG S+GEIV RG S+MLGY KD + T+  
Sbjct: 361 RARLKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAAC 420

Query: 421 MSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVA 480
           M EDGWF++GD+GV+H DGY+EIKDRSKDVII GGEN+SS E+E+VLY NP+V EAAVVA
Sbjct: 421 MREDGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFISLK---EGLSRKP-TEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTA 540
           +PD+ WGETPCAF+SLK    G    P TE EI E+CK KLPK+MVP+ VIF  E+PKT+
Sbjct: 481 KPDKMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTS 540

Query: 541 TGKFQKFVLRQKAKSL 550
           TGK QKF+LRQ AK+L
Sbjct: 541 TGKIQKFLLRQMAKTL 548

BLAST of CsaV3_5G020280 vs. TrEMBL
Match: tr|A0A0A0KMT3|A0A0A0KMT3_CUCSA (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGPPQKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of CsaV3_5G020280 vs. TrEMBL
Match: tr|A0A1S3CIL0|A0A1S3CIL0_CUCME (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 527/557 (94.61%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of CsaV3_5G020280 vs. TrEMBL
Match: tr|A0A1S3CIM6|A0A1S3CIM6_CUCME (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501383 PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 4.9e-256
Identity = 429/557 (77.02%), Postives = 491/557 (88.15%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           M+ LKP PANS PLTPI F+ERA+++Y DS SIIYNST++TWS+T+ RCL+LASS+SS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I +GHVVSV +PNTP MYEL F+VPMAGAIL+ INLRLD RTIS+ LRHSESKL+FVDQ 
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S ALI DAL LFPP+ KRPLLVLI DD A +DS+  S V+I  +FVD YE+M++ GD +F
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSA--SSVAIGGDFVDFYEDMIQKGDPEF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDPIV+NYTSGTTS+PKGVV+ HRG+FTV LDS++EWGVPK SVYLWTLPMF
Sbjct: 181 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFP+ IAAVGGTN+CIRKFD+ LIFSLI RHHVTHLCGAPVVLNMLTNSP+N PL
Sbjct: 241 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           +RPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +I CSWK +WNQLPA E+AR
Sbjct: 301 ERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLD ET KSVKRDG SIGEIV+RG S+MLGY KD EAT KAM+E
Sbjct: 361 LKARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SL+EGLSR PTEEEIIEYC+GKL  FMVPKTV+FM ++PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           +LR+ AKS+GP +KSRL
Sbjct: 541 LLREMAKSMGPSRKSRL 555

BLAST of CsaV3_5G020280 vs. TrEMBL
Match: tr|A0A0A0KT33|A0A0A0KT33_CUCSA (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.1e-255
Identity = 432/557 (77.56%), Postives = 490/557 (87.97%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           ME LKP PANS PLTPIGF+ERA++IY DS SIIY+STT+TWS+T+RRCL+LASS+SS G
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I +GHVVSV APNTP MYEL FAVPMAGAIL+ INLRLD RTIS+ LRHSESKL+FVDQ 
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S  LI DALALFPP+ KRPLLVLI+DD A +DS+  S V+I  + VD YE+MV+ GD +F
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSA--SSVAIGGDVVDLYEDMVQKGDPEF 201

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDPIV+NYTSGTTS+PKGVV+ HRG+FTV LDS++EWGVPK SVYLWTLPMF
Sbjct: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFP+ IAAVGGTN+CIRKFD+ LIFSLI RHHVTH+CGAPVVLNMLTNSP+N PL
Sbjct: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           +RPVKILTAGAPPPA+VLLRTESLGF V+HGYGLTE+ G +I CSWK EWNQLPA E+AR
Sbjct: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLD  T K V+RDG SIGEIV+RG S+MLGY KD EAT KAM+E
Sbjct: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SL+EGL+R PTEEEIIEYCKGKL  FMVPKTV+FM ++PKT+TGK QKF
Sbjct: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561

Query: 541 VLRQKAKSLGPPQKSRL 558
           +LR+ AKS+G   KSRL
Sbjct: 562 LLREMAKSMGCSPKSRL 576

BLAST of CsaV3_5G020280 vs. TrEMBL
Match: tr|A0A2P5CQX7|A0A2P5CQX7_PARAD (2,3-dihydroxybenzoate-AMP ligase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_131240 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 2.7e-238
Identity = 387/556 (69.60%), Postives = 473/556 (85.07%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           ME+LKPRPANS PLTP+GF+ERAA +YGD TSIIY++ +YTWSETHRRCL+LASSI+S G
Sbjct: 1   MEELKPRPANSSPLTPLGFLERAATVYGDCTSIIYDAVSYTWSETHRRCLRLASSITSLG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I+RGHVVSV APN P MYELHFAVPM+GA+LN +NLRLD RTIS+LLRHSESKLIFVD  
Sbjct: 61  IRRGHVVSVLAPNVPQMYELHFAVPMSGAVLNAVNLRLDARTISVLLRHSESKLIFVDYL 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S  LI +A+ALFP     P LVL+ DD    D++   V S+   F  SY+++++ GD  F
Sbjct: 121 SRDLILEAIALFPGGAPVPRLVLMADDA---DAAAAVVGSVGAEFCCSYKDLIEKGDPDF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +W+RP SEWDP++LNYTSGTTS+PKGVV+CHRG+F + +DS+++WGVPKQ VYLWTLPMF
Sbjct: 181 EWVRPESEWDPMILNYTSGTTSSPKGVVHCHRGIFIITVDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWS+PWG+AAVGGTN+C+R+FD+  IF LIRRH VTH+CGAPVVLNML N+P + PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRRFDAPAIFDLIRRHGVTHMCGAPVVLNMLCNAPGSEPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
            RPV+++TAGAPPPA+VL RTESLGFVV+HGYGLTE+AG ++ C+WK EWN+LPA E+AR
Sbjct: 301 KRPVQVMTAGAPPPAAVLFRTESLGFVVSHGYGLTETAGLVVSCAWKREWNRLPATERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGV  +A+  +DV+DP T   VKRDG+++GE+V+RG S+MLGYLKD E T+K+MS 
Sbjct: 361 LKARQGVGTVALTRLDVVDPATGAGVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMSG 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWF+TGDVGV+HPDGY+EIKDRSKDVIISGGENLSSVEVESVLY  P VNEAAVVARPD
Sbjct: 421 DGWFYTGDVGVLHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYGFPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SLKEGL +KPTE+E+IEYC+ KLP +MVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKEGLGKKPTEKEVIEYCRSKLPHYMVPKTVVFKDELPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSR 557
           +LR  A+++G  +  R
Sbjct: 541 ILRDLARAMGSARAGR 553