CsaV3_5G018610 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_5G018610
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	Proline--tRNA ligase
Location	chr5 : 14184962 .. 14190996 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGTGAGATGGGAGTTCAGCCATCCCACACCATTTATTAGGCAATTACTTTTGCTTGTGCTTATCATTTTGTTTCTTTTACTTTCACCATGTTGATGGACTAAGTTGGACCTGTTAACAGTATAATCTTCTTATTTTGTTTTTAACTTTATTTTAAATCGTTTGGAGAGAAGACTTAATTTTATTAACCACTGACTGCAGGAGTCGTGAGTTCCTATGGCAGGAAGGGCACACTGCTTTTGCTACTAAGGATGAAGCAGATACAGAGGTATCAAACTCTAATTTTGAATTATAATTTAGAAAAATGTCTTTGTTATGATTAGCTTTAGGTTTTAAAACCAACTTATTGTTTTCTAGTTACCAGTGATGAAACTGAGTCTATCCTGGGGATTATTGCATTGAATTTGAATCTATCTTTGTTATATAGTCACTCTACTGGATTGACTTTTTTGCAAAACTTAAGTCGTTTGTAGATTTAAGCATCCTGTTCAGCTGCAAGTTCTGTTTTAATAATAATAAGATCTTTAGAATATAATAAACTTGTTTTGTTGATTTGTTTGATGTCACAAGGAAGTACTGTGGAGTCTTAAGATAAGGTAACTGTGAAGGCGTGCAACGTTGATCTTTTAGAACTTTTGAGATGTAGGGCCAGTGGGTCACCGATTTACCAGTTACCACTCACCACAGAATCAATGCTACTATAATTATAAAACATGAAGTTTACTATCACGAGCAAATTTGGAGATTCAAACATGCAAAACTCACTTTAATTGGAAGCAAAAGGAATGAGTGGTCTATAGTGGCAGCATTGAGTTCACGTGATCATCCTGAAGTGCAGGCTTTGAACTCAACGATGAGGTGTGCATTTTGTTCTGTTTAAAGCATGATAGTGTAATTTAGGTTTCATTTGCCGCATACATTTTTGGATTTTATATATGATTGGCTATTTTTTAAGAGTCAAAACTGGATTTCTTTTCTTTTTTAAGTTTGGAGATTGTTAATGAATTCTTTTAGAGGTTTTTTTTCTTTCTTTTTTTAATTTTTATCTTTTTATTTAAGTCATGTGCTTTATATTTTTGCCATGTGTTAGGTCTAATTAAATTTTTTTGCATCATGAACCATGTTCAAATGTTCATAGATGAACCAAGCGGTGAAGCTTTTATGCTTCTGTTTTCTCCTTTTTCCTTTTTGGTAGAAAACACAGTTATATAAACAATTGGAAGAATATAAAGGGGAGAGGGAAGGTTAAATCTCCATCAAATTCTTAACTAAGCTACGGGAGATTACCAAAATACCCTTCAATCTAAGTCTGAAATTTTGCAGTTCTTTTGTGTAACTGTGATCATTATCTAAAGGTCATTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGACGCATATATGAAGAATACCTGGTGATTCCTGTTATTAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGTATAAGTTGACTAAATTGCTATGACCCATCTTCAGCATGGCAAATTGATATCCATCACTTGTGTAAGTGGTATAAAACCATTGTTCCGTTTGTGATATGCAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAATTGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGGTAATATTTTGATACTTGCATTTGTCGTAGCCTTTGGTAACTTGTTAGAGTGAATTTCTGCGTCTTGTTAATCTAGCCTTCCAAACTGTTTGTAGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCTCCAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCAAGGAATTTTTTATGCTTGTTCTGCCACTTCGGATATGTTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACATTGGGGAGAACTATTCTCCTGGATGGAAATACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCCAAGGACTTAGCAAACAATCAGGTTAGATATTTTGTCTATTAATGTTCTTTTCTGTCTACTGAAATACTGGTTTCTCCTTCCTAATAATTATAAATGTCTGATTTGGATTGTGTTGATGCATAGTGCTAATCTATAATTGTATGCTTTCTTTTTCTAGGTACGCGCAGTTCGCCGTGATAATTCTGCAAAGAAGGACATACCCAACCCTTCACTCTTGTTCATAGTGATGTCTGGGGACCATCCAAGATAACAACCTCATCTGAAAAACGGTGGTTCGTAACCTTCATTGATGATCATACCCGTCTTACCTGGGTCTACCTTATCACTGATAAATCTGAGGTTTCCTCGTATGTTTCAAAATTTCTATCACACCATTGAAACACAATTTCATCAAAAAATTGCTATTCTTCGGAGTGATAATGGTCGGGAATTCCAAAACCATAACCTTAGTGAATTTCTTGCTTCCAAAGGGATTGTTCATCAAAACTCGTGCGCCTACACTCCTCAACAAAATGGAGTGGCGAGCGAAAAAACTGTCACCTTCTGGAAGTAGCCCGTTCCCTTATGCTTTCTACTTCCCTTCCTTCATACTTGTGGGGAGATGCTATTCTTACAGCAGCTCATTTAATCAATAGAATGCCTTCTCGTATTCTTCATCTTCAAACTCCCTTAGATTGTCTTAATGAGTCCTACCCATCGACTCGTCATGTTTCTGAGGTTCCTCTTCGTGTGTTTGGGTGTACCGCTTATGTCCATAATTTTGGCCCTAATCAAACCAAATTTACCCCTCGGGCTCAGGCATGTGTGTTTGTTGGGTATCCCCCTCACCAGCGTGGTTATAAATGTTTTCACCCACCATCCAGAAAATACTTTGTCACTATGGATGTTACTTTCTGTGAGGATCGACCCTACTTTCCCGTTAGCCATCTTCAGGGGGAGAGTGTGAGTGAAGAGTCTAACAACACCTTTGAATTCATCGAACCCACTCCTAGTGTCGTGTCTAACATCATTCCTCATTCCATAGTCCTACCCACAAACCAAGTCCCCTGGAAAACGTACTACAGGAGGAATCACAAAAAGGAAGTCGGTTCCCCTACTAGTCAGCCGCCGGCTCTAGTCCAAGACTCTGAACCTCCTCGAGATCAAGGTATGGAAAACCCTACTGAACCTTGTACTAAGAATATGATAAGTGAGAATGACAGGTCTAATGTTGCTGTTCTTAAAAACGTGGAAGAAAAGGACAGTGGTGATGAGATTGAGGTCAGAATAGAAACTCGTAATAATGAAGCGGAACAGGGTCATACAGGAAAATCAGATGATTATGATTCCTCTCTTGACATTCCCATTGCTCTGGGAAAAGGGACCAGGTCTTGTACTAAACACCCCATTTGCAATTATGTTTCCTACGATAATCTCTCTCCTCAGTTCAGAGTTTTTACAGCAAGCCTTGACTCTACCATAATACCAAAAGATATCTACACTGCTTTAAAGTGTCCTGAATGGAAGAATGATGTCATGAAAGAGATGAAAGCTCTTGAAAAGAATAGTACTTGGGACATTTGTACTCTACCTAAGGGACACAAAACTGTGGGATGCAAATGGGTGTTCTCTCTCAAATACAAAGCTGATGGTACTCTTGACAGACACAAGGCAAGGTTAGTTGCGAAGGGATTTACTCAAACCTACGGTATTGACTATTCAGAAACTTTTTCTCCAGTTGCTAAGTTGAATACTATTATAGTTCTGTTATCTGTTGCTGTGAACAAGGATTGGCCTCTATATCAGCTGGATGTTAAGAATGTCTTTTTGATTGGAGACCTCGTAGAGGAAGTCTACATGAGCCATCCGCCTGGATTTGAAGCCCAGTTTGGTCAGCATGTGTGTAAACTCCAGAAATCTATATATGGTCTGAAACAGTCTCCCAGAGCATGGTTTGACAGATTCACTACCTTTGTCAAGTTCCAAGGGTATAGGCAGGGACACTCTGATCATACTTTATTTACAAAGGTTTCCAAAACAGGAAAGATTGCTGTTCTAATAGTTTATGTGGGTGGCATTGTTTTGACTGGAGATGATCAGGCGGAAATCAGTCAACTAAAGCAGAGAATGGGCGATGAGTTTGAAATCAAGGATTTGGGAAATTTGAAATATTTCCTTGGAATGGAGGTGGCCAGATCTAAAGAAGGTATCTCCATATCTCAAAGAAAATACATCCTTGATTTGTTAACCAAGACAGGTATGTTAGGATGTCGTTCCACTGACACTCCTATTGAATTCAACTGCAAACTAGGAAACTCTGATGATCAAGTTCCAGTTGATAAAGAACAATATCAACGCCTCGTGGGTAAATTAATTTACTTATCCCATACTCGTCCTGATATTTCCTTTGCTGTGAGTGTTGTCAGCCAGTTTATGCAGACCCCTAATGAGGAACACATGAAAGCTGTCAACAGAATCTTGAGATACTTAAAATCAACACCTGGTAAAGGGCTGATGTTTAGAAAAACAGACAGAAAGACCATTGAGGCATACACTGACTCGGATTGGGCAGGATCTGTTGTTGACAGAAAATCTACCTCTGGTTATTGTACCTTTGTTTGGGGCAATCTTGTAACTTGGAGGAGTAAGAAGCAAAGTGTTGTGGCCAGGAGCAGCGCTGAGGCTGAATACAGAGCTATGAGTTTAGGAATATGTGAGGAAATTTGGCTTCATAAAGTTTTGACAGATCTTCATCAGGAATGTGAGACACCATTGAAGCTTTTCTGTGATAATAAAGCCGCTATTAGTATTGCTAACAACCCTGTTCAACATGATAGAACTAAACATGTTGAGATTGATCGACATTTTATCAAAGAAAAACTTGACAGTGGGAGCATATGCATTCCGTACATCCCTTCGAGTCAACAGGTTGCTGATGTTCTTACCAAAGGGCTTCTCAGACCAAACTTCGACTTTTGCGTTAGCAAGTTGGGCCTCATTGATATTTACGTCCCAACTTGAGGGGGAGTGTTGGAATTCTTTCCTTTTTTTCTAGGCTTTTTCCTTGTTAGAATCCTAGAATAATTATGGAAAGATTATGGGGATGTATTCCTTATTTTCCTAAATCTTTTCCTTTTTTATTCCATTGTGTACTCTATTTATTCTCCCATGTACCTATTGTTTTATTCATTAGAAAATAATAACAACAAACAATCGTGGTTTTTCTCTCGGTACTCGGGTTTCCACGTAAATTGGTGTGAACTCGTTGTCTCTCTTTTCAATACTTTCATACGCTGAAATCTATTTGATGAATCAACTATAGACTCCTGGTTTGTAGAGCTTGTTTTTGTAGCTAGGAAGGGAGATTTTGTTGCAATATTAGCTGCTGCAGCGTAAATAATGTTTCATGAGTTTAGGATGATTTCTATAGAATTGATAGGGTAATTATCAATAAAATTTTGAACTGCATTGATCACTGGGTTGAGTCGATGCATTTTTAAGTATTTAGTTTCTACTGAAGTCTATGTTAGCTATGTGGTAAATGGAAATAAGTTTTCTGAAATCTGAAATACTTAATATATCTGTTGTATGCCATCCTCTATCATTTACTAGTGTATTTCTAAAGGATGATGGTTTCATTTGTTATGTTACTCCAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGATGGGAGCAGCGAAAACCCTATGCTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGGTTACTAAATTCATGTCTAAATCTGTCTTCATTTTACATGTTAATATGAGTTTATTTCAACTTGTGGGATTTTGGGAAACTTGATAGCACTCTTTTAGTTCCTTGAATTTACTATGATGTTTGTGCTTAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAA

mRNA sequence

ATGTTGAGTCGTGAGTTCCTATGGCAGGAAGGGCACACTGCTTTTGCTACTAAGGATGAAGCAGATACAGAGGGCCAGTGGGTCACCGATTTACCAGTTACCACTCACCACAGAATCAATGCTACTATAATTATAAAACATGAAGTTTACTATCACGAGCAAATTTGGAGATTCAAACATGCAAAACTCACTTTAATTGGAAGCAAAAGGAATGAGTGGTCTATAGTGGCAGCATTGAGTTCACGTGATCATCCTGAAGTGCAGGCTTTGAACTCAACGATGAGTTTGGAGATTGTTCTTGAGATATTGGAACTGTATAGACGCATATATGAAGAATACCTGGTGATTCCTGTTATTAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAATTGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCTCCAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCAAGGAATTTTTTATGCTTGTTCTGCCACTTCGGATATGTTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACATTGGGGAGAACTATTCTCCTGGATGGAAATACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCCAAGGACTTAGCAAACAATCAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGATGGGAGCAGCGAAAACCCTATGCTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAA

Coding sequence (CDS)

ATGTTGAGTCGTGAGTTCCTATGGCAGGAAGGGCACACTGCTTTTGCTACTAAGGATGAAGCAGATACAGAGGGCCAGTGGGTCACCGATTTACCAGTTACCACTCACCACAGAATCAATGCTACTATAATTATAAAACATGAAGTTTACTATCACGAGCAAATTTGGAGATTCAAACATGCAAAACTCACTTTAATTGGAAGCAAAAGGAATGAGTGGTCTATAGTGGCAGCATTGAGTTCACGTGATCATCCTGAAGTGCAGGCTTTGAACTCAACGATGAGTTTGGAGATTGTTCTTGAGATATTGGAACTGTATAGACGCATATATGAAGAATACCTGGTGATTCCTGTTATTAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAATTGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCTCCAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCAAGGAATTTTTTATGCTTGTTCTGCCACTTCGGATATGTTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACATTGGGGAGAACTATTCTCCTGGATGGAAATACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCCAAGGACTTAGCAAACAATCAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGATGGGAGCAGCGAAAACCCTATGCTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAA

Protein sequence

MLSREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAKLTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY

Homology

[Close]

BLAST of CsaV3_5G018610 vs. NCBI nr
Match: XP_008466310.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_008466350.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_016898851.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo])

HSP 1 Score: 458.0 bits (1177), Expect = 2.8e-125
Identity = 252/408 (61.76%), Postives = 256/408 (62.75%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 178 SREFLWQEGHTAFATKDEADTE-------------------------------------- 237

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 238 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 297

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFENEKGEKAMVWQN
Sbjct: 298 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQN 357

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLVMP +VAS+QVIIVPVPYKDADT+GIF ACSATSD LS
Sbjct: 358 SWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALS 417

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           KAGIRAEVDI ENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 418 KAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRA 477

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 478 LLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEK 511

[Close]

BLAST of CsaV3_5G018610 vs. NCBI nr
Match: XP_008466343.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo])

HSP 1 Score: 458.0 bits (1177), Expect = 2.8e-125
Identity = 252/408 (61.76%), Postives = 256/408 (62.75%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 128 SREFLWQEGHTAFATKDEADTE-------------------------------------- 187

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 188 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 247

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFENEKGEKAMVWQN
Sbjct: 248 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQN 307

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLVMP +VAS+QVIIVPVPYKDADT+GIF ACSATSD LS
Sbjct: 308 SWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALS 367

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           KAGIRAEVDI ENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 368 KAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRA 427

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 428 LLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEK 461

[Close]

BLAST of CsaV3_5G018610 vs. NCBI nr
Match: XP_022956898.1 (proline--tRNA ligase, cytoplasmic [Cucurbita moschata])

HSP 1 Score: 446.4 bits (1147), Expect = 8.4e-122
Identity = 245/408 (60.05%), Postives = 252/408 (61.76%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 178 SREFLWQEGHTAFATKDEADTE-------------------------------------- 237

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 238 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 297

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFENEKGEKAMVWQN
Sbjct: 298 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQN 357

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QV+IVPVPYKDADTQGIF ACS T D LS
Sbjct: 358 SWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLS 417

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           +AGIRAEVD  +NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 418 EAGIRAEVDARDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRA 477

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 478 SLVEQVKELLDSIQQSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEK 511

[Close]

BLAST of CsaV3_5G018610 vs. NCBI nr
Match: XP_022955643.1 (proline--tRNA ligase, cytoplasmic isoform X1 [Cucurbita moschata])

HSP 1 Score: 446.4 bits (1147), Expect = 8.4e-122
Identity = 246/408 (60.29%), Postives = 252/408 (61.76%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 177 SREFLWQEGHTAFATKDEADTE-------------------------------------- 236

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 237 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 296

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFENEKGEKAMVWQN
Sbjct: 297 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQN 356

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QVIIVPVPYKDADTQGIF ACS T D LS
Sbjct: 357 SWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLS 416

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           +AGIRAEVD  +NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 417 EAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRA 476

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 477 ALVEQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEK 510

[Close]

BLAST of CsaV3_5G018610 vs. NCBI nr
Match: XP_022955644.1 (proline--tRNA ligase, cytoplasmic isoform X2 [Cucurbita moschata])

HSP 1 Score: 446.4 bits (1147), Expect = 8.4e-122
Identity = 246/408 (60.29%), Postives = 252/408 (61.76%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 128 SREFLWQEGHTAFATKDEADTE-------------------------------------- 187

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 188 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 247

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFENEKGEKAMVWQN
Sbjct: 248 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQN 307

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QVIIVPVPYKDADTQGIF ACS T D LS
Sbjct: 308 SWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLS 367

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           +AGIRAEVD  +NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 368 EAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRA 427

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 428 ALVEQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEK 461

[Close]

BLAST of CsaV3_5G018610 vs. TAIR10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein)

HSP 1 Score: 398.7 bits (1023), Expect = 3.7e-111
Identity = 215/408 (52.70%), Postives = 239/408 (58.58%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATK EAD E                                      
Sbjct: 197 SREFLWQEGHTAFATKAEADEE-------------------------------------- 256

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VL+ILELYRRIYEEYL +PV+KG 
Sbjct: 257 ------------------------------------VLQILELYRRIYEEYLAVPVVKGM 316

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAK+FEINFENEK E  MVWQN
Sbjct: 317 KSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQN 376

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVM+M HGDDKGLV+P +VAS+QV+++PVPYKDA+TQGI+ AC+AT+  L 
Sbjct: 377 SWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALC 436

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           +AGIRAE D+ +NYSPGWKYS WEMKGVPLRIEIGP+DL N+Q                 
Sbjct: 437 EAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRG 496

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVE+
Sbjct: 497 SLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVER 530

[Close]

BLAST of CsaV3_5G018610 vs. TAIR10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein)

HSP 1 Score: 189.9 bits (481), Expect = 2.6e-48
Identity = 87/187 (46.52%), Postives = 129/187 (68.98%), Query Frame = 0


Query: 101 EILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQN 160
           +++E+Y R   E   IPVI G+KS++E FAG   T ++EA + +  + +Q  TSH LGQN
Sbjct: 236 QMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDR-KALQAGTSHNLGQN 295

Query: 161 FAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPV 220
           F++ F   F +E GE+  VWQ SWA STR +G ++M HGDD GL++P ++A IQV+IVP+
Sbjct: 296 FSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVVIVPI 355

Query: 221 PYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKD 280
             KD +  G+  A S+  + L  AG+R ++D  +  +PGWK++ WEMKG+PLRIEIGP+D
Sbjct: 356 WKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMKGIPLRIEIGPRD 415

Query: 281 LANNQEV 288
           +++N  V
Sbjct: 416 VSSNSVV 421

[Close]

BLAST of CsaV3_5G018610 vs. TAIR10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related)

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-37
Identity = 94/221 (42.53%), Postives = 108/221 (48.87%), Query Frame = 0


Query: 196 MVHGDDKGLVMPLQVASIQVIIVPVPYKD-ADTQGIFYACSATSDMLSKAGIRAEVDIGE 255
           M HGDDKGLV P +VA +QV+++ VP K  AD Q +  AC A    L  AGIRAE DI +
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 256 NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------------------- 315
           NYS GWKY+  E+ GVPLRIE GP+DLAN+Q                             
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIEQVKDXXXX 208

Query: 316 --------------------------------------------EVEKDVKTRTKGE--- 338
                                                       EVEKDVK RT+G+   
Sbjct: 209 XXXXXXXXXXXXXXXXXXXXXTWDEFVEALSQKKLILAPWCDKVEVEKDVKRRTRGDETX 268

[Close]

BLAST of CsaV3_5G018610 vs. Swiss-Prot
Match: sp|Q9M1R2|SYPC_ARATH (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 6.6e-110
Identity = 215/408 (52.70%), Postives = 239/408 (58.58%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATK EAD E                                      
Sbjct: 197 SREFLWQEGHTAFATKAEADEE-------------------------------------- 256

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VL+ILELYRRIYEEYL +PV+KG 
Sbjct: 257 ------------------------------------VLQILELYRRIYEEYLAVPVVKGM 316

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAK+FEINFENEK E  MVWQN
Sbjct: 317 KSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQN 376

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVM+M HGDDKGLV+P +VAS+QV+++PVPYKDA+TQGI+ AC+AT+  L 
Sbjct: 377 SWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALC 436

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           +AGIRAE D+ +NYSPGWKYS WEMKGVPLRIEIGP+DL N+Q                 
Sbjct: 437 EAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRG 496

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVE+
Sbjct: 497 SLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVER 530

[Close]

BLAST of CsaV3_5G018610 vs. Swiss-Prot
Match: sp|Q8I5R7|SYP_PLAF7 (Proline--tRNA ligase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=proRS PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.5e-61
Identity = 130/331 (39.27%), Postives = 173/331 (52.27%), Query Frame = 0


Query: 91  NSTMSLEIVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQ 150
           N   ++++V +IL+LYRR YEEYL +P+IKG KSE EKF G  +T++ EAFI   GR IQ
Sbjct: 416 NEEEAVKLVFDILDLYRRWYEEYLAVPIIKGIKSEGEKFGGANFTSTAEAFISENGRAIQ 475

Query: 151 GATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQV 210
            ATSH LG NFAK+F+I FE+E   K  V Q SW  +TR+IG+M+M HGDDKGLV+P  V
Sbjct: 476 AATSHYLGTNFAKMFKIEFEDENEVKQYVHQTSWGCTTRSIGIMIMTHGDDKGLVLPPNV 535

Query: 211 ASIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGV 270
           +  +V+IVP+ YK  D   I   C     +L  A I    D   +YSPG+K++HWE++G+
Sbjct: 536 SKYKVVIVPIFYKTTDENAIHSYCKDIEKILKNAQINCVYDDRASYSPGYKFNHWELRGI 595

Query: 271 PLRIEIGPKDLANN---------------------------------------------- 330
           P+RIE+GPKDL NN                                              
Sbjct: 596 PIRIEVGPKDLQNNSCVIVRRDNNEKCNVKKESVLLETQQMLVDIHKNLFLKAKKKLDDS 655

Query: 331 -------------------------------QEVEKDVK----TRTKGE---MGAAKTLC 338
                                          +E++K+ +     +T  E    GA K LC
Sbjct: 656 IVQVTSFSEVMNALNKKKMVLAPWCEDIATEEEIKKETQRLSLNQTNSETTLSGAMKPLC 715

[Close]

BLAST of CsaV3_5G018610 vs. Swiss-Prot
Match: sp|Q8CGC7|SYEP_MOUSE (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs PE=1 SV=4)

HSP 1 Score: 226.5 bits (576), Expect = 4.5e-58
Identity = 126/291 (43.30%), Postives = 165/291 (56.70%), Query Frame = 0


Query: 3    SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
            +REFLWQEGH+AFAT +EA  E                                      
Sbjct: 1164 TREFLWQEGHSAFATFEEAADE-------------------------------------- 1223

Query: 63   LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                                VL+ILELY R+YEE L IPV++G+
Sbjct: 1224 ------------------------------------VLQILELYARVYEELLAIPVVRGR 1283

Query: 123  KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEK--GEKAMVW 182
            K+E EKFAGG YTT++EAFI  +GR IQGATSH LGQNF+K+ EI FE+ K  GEK   +
Sbjct: 1284 KTEKEKFAGGDYTTTIEAFISASGRAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAY 1343

Query: 183  QNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDA----DTQGIFYACSA 242
            Q SW  +TRTIGVMVMVHGD+ GLV+P +VAS+QV+++P    +A    D + +   C+ 
Sbjct: 1344 QCSWGLTTRTIGVMVMVHGDNMGLVLPPRVASVQVVVIPCGITNALSEEDREALMAKCNE 1380

Query: 243  TSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQEV 288
                L  A IR  VD+ +NYSPGWK++HWE+KGVP+R+E+GP+D+ + Q V
Sbjct: 1404 YRRRLLGANIRVRVDLRDNYSPGWKFNHWELKGVPVRLEVGPRDMKSCQFV 1380

[Close]

BLAST of CsaV3_5G018610 vs. Swiss-Prot
Match: sp|P28668|SYEP_DROME (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)

HSP 1 Score: 226.1 bits (575), Expect = 5.9e-58
Identity = 129/290 (44.48%), Postives = 160/290 (55.17%), Query Frame = 0


Query: 3    SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
            +REFLWQEGHTAFA K+EA  E                                      
Sbjct: 1365 TREFLWQEGHTAFADKEEAAKE-------------------------------------- 1424

Query: 63   LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                                VL+IL+LY  +Y   L IPV+KG+
Sbjct: 1425 ------------------------------------VLDILDLYALVYTHLLAIPVVKGR 1484

Query: 123  KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFEN-EKGEKAMVWQ 182
            K+E EKFAGG YTT+VEAFI  +GR IQGATSH LGQNF+K+FEI +E+ E  +K  V+Q
Sbjct: 1485 KTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQQKKYVYQ 1544

Query: 183  NSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVP----VPYKDADTQGIFYACSAT 242
            NSW  +TRTIGVM+MVH D++GLV+P  VA IQ I+VP    V  KD +   +  AC A 
Sbjct: 1545 NSWGITTRTIGVMIMVHADNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKAL 1580

Query: 243  SDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQEV 288
               L   G+R E D  +NYSPGWK++HWE+KGVPLR+E+GPKDL   Q V
Sbjct: 1605 EKRLVGGGVRCEGDYRDNYSPGWKFNHWELKGVPLRLEVGPKDLKAQQLV 1580

[Close]

BLAST of CsaV3_5G018610 vs. Swiss-Prot
Match: sp|P07814|SYEP_HUMAN (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS PE=1 SV=5)

HSP 1 Score: 225.3 bits (573), Expect = 1.0e-57
Identity = 123/291 (42.27%), Postives = 162/291 (55.67%), Query Frame = 0


Query: 3    SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
            +REFLWQEGH+AFAT +EA  E                                      
Sbjct: 1164 TREFLWQEGHSAFATMEEAAEE-------------------------------------- 1223

Query: 63   LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                                VL+IL+LY ++YEE L IPV+KG+
Sbjct: 1224 ------------------------------------VLQILDLYAQVYEELLAIPVVKGR 1283

Query: 123  KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEK--GEKAMVW 182
            K+E EKFAGG YTT++EAFI  +GR IQG TSH LGQNF+K+FEI FE+ K  GEK   +
Sbjct: 1284 KTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAY 1343

Query: 183  QNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDA----DTQGIFYACSA 242
            QNSW  +TRTIGVM MVHGD+ GLV+P +VA +QV+I+P    +A    D + +   C+ 
Sbjct: 1344 QNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCND 1380

Query: 243  TSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQEV 288
                L    IR   D+ +NYSPGWK++HWE+KGVP+R+E+GP+D+ + Q V
Sbjct: 1404 YRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFV 1380

[Close]

BLAST of CsaV3_5G018610 vs. TrEMBL
Match: tr|A0A1S4DS87|A0A1S4DS87_CUCME (proline--tRNA ligase, cytoplasmic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.9e-125
Identity = 252/408 (61.76%), Postives = 256/408 (62.75%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 178 SREFLWQEGHTAFATKDEADTE-------------------------------------- 237

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 238 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 297

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFENEKGEKAMVWQN
Sbjct: 298 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQN 357

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLVMP +VAS+QVIIVPVPYKDADT+GIF ACSATSD LS
Sbjct: 358 SWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALS 417

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           KAGIRAEVDI ENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 418 KAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRA 477

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 478 LLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEK 511

[Close]

BLAST of CsaV3_5G018610 vs. TrEMBL
Match: tr|A0A1S3CR78|A0A1S3CR78_CUCME (proline--tRNA ligase, cytoplasmic isoform X4 OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.9e-125
Identity = 252/408 (61.76%), Postives = 256/408 (62.75%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 128 SREFLWQEGHTAFATKDEADTE-------------------------------------- 187

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 188 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 247

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFENEKGEKAMVWQN
Sbjct: 248 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQN 307

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLVMP +VAS+QVIIVPVPYKDADT+GIF ACSATSD LS
Sbjct: 308 SWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALS 367

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
           KAGIRAEVDI ENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 368 KAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRA 427

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 428 LLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEK 461

[Close]

BLAST of CsaV3_5G018610 vs. TrEMBL
Match: tr|A0A0A0KTZ1|A0A0A0KTZ1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.6e-121
Identity = 245/408 (60.05%), Postives = 252/408 (61.76%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 178 SREFLWQEGHTAFATKDEADTE-------------------------------------- 237

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 238 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 297

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFENEKGEKAMVWQN
Sbjct: 298 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQN 357

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QVIIVPVPYKDADTQGIF ACSAT D L+
Sbjct: 358 SWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLT 417

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
            AGIRAEVD  +NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 418 AAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRD 477

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 478 SLVEQVKELLESIQQSLFDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEK 511

[Close]

BLAST of CsaV3_5G018610 vs. TrEMBL
Match: tr|A0A1S4DZZ0|A0A1S4DZZ0_CUCME (proline--tRNA ligase, cytoplasmic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494536 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 3.6e-121
Identity = 244/408 (59.80%), Postives = 252/408 (61.76%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 128 SREFLWQEGHTAFATKDEADTE-------------------------------------- 187

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 188 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 247

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFEN+KGEKAMVWQN
Sbjct: 248 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQN 307

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QVIIVPVPYKDADTQGIF ACSAT D L+
Sbjct: 308 SWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLT 367

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
            AGIRAEVD  +NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 368 DAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRD 427

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 428 SLVEQVKELLESIQQSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEK 461

[Close]

BLAST of CsaV3_5G018610 vs. TrEMBL
Match: tr|A0A1S3BXN6|A0A1S3BXN6_CUCME (proline--tRNA ligase, cytoplasmic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494536 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 3.6e-121
Identity = 244/408 (59.80%), Postives = 252/408 (61.76%), Query Frame = 0


Query: 3   SREFLWQEGHTAFATKDEADTEGQWVTDLPVTTHHRINATIIIKHEVYYHEQIWRFKHAK 62
           SREFLWQEGHTAFATKDEADTE                                      
Sbjct: 178 SREFLWQEGHTAFATKDEADTE-------------------------------------- 237

Query: 63  LTLIGSKRNEWSIVAALSSRDHPEVQALNSTMSLEIVLEILELYRRIYEEYLVIPVIKGK 122
                                               VLEILELYRRIYEEYL IPVIKGK
Sbjct: 238 ------------------------------------VLEILELYRRIYEEYLAIPVIKGK 297

Query: 123 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQN 182
           KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAK+FEINFEN+KGEKAMVWQN
Sbjct: 298 KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQN 357

Query: 183 SWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLS 242
           SWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QVIIVPVPYKDADTQGIF ACSAT D L+
Sbjct: 358 SWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLT 417

Query: 243 KAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ----------------- 302
            AGIRAEVD  +NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQ                 
Sbjct: 418 DAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRD 477

Query: 303 --------------------------------------------------------EVEK 338
                                                                   EVEK
Sbjct: 478 SLVEQVKELLESIQQSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEK 511

The following BLAST results are available for this feature:

BLAST of CsaV3_5G018610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008466310.1	2.8e-125	61.76	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_00846...	[more]
XP_008466343.1	2.8e-125	61.76	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo]	[more]
XP_022956898.1	8.4e-122	60.05	proline--tRNA ligase, cytoplasmic [Cucurbita moschata]	[more]
XP_022955643.1	8.4e-122	60.29	proline--tRNA ligase, cytoplasmic isoform X1 [Cucurbita moschata]	[more]
XP_022955644.1	8.4e-122	60.29	proline--tRNA ligase, cytoplasmic isoform X2 [Cucurbita moschata]	[more]

BLAST of CsaV3_5G018610 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G62120.1	3.7e-111	52.70	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G52520.1	2.6e-48	46.52	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G10880.1	1.2e-37	42.53	tRNA synthetase-related / tRNA ligase-related	[more]

BLAST of CsaV3_5G018610 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9M1R2\|SYPC_ARATH	6.6e-110	52.70	Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P...	[more]
sp\|Q8I5R7\|SYP_PLAF7	1.5e-61	39.27	Proline--tRNA ligase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=proRS PE...	[more]
sp\|Q8CGC7\|SYEP_MOUSE	4.5e-58	43.30	Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs PE=...	[more]
sp\|P28668\|SYEP_DROME	5.9e-58	44.48	Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G...	[more]
sp\|P07814\|SYEP_HUMAN	1.0e-57	42.27	Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS PE=1...	[more]

BLAST of CsaV3_5G018610 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4DS87\|A0A1S4DS87_CUCME	1.9e-125	61.76	proline--tRNA ligase, cytoplasmic isoform X1 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
tr\|A0A1S3CR78\|A0A1S3CR78_CUCME	1.9e-125	61.76	proline--tRNA ligase, cytoplasmic isoform X4 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
tr\|A0A0A0KTZ1\|A0A0A0KTZ1_CUCSA	1.6e-121	60.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1	[more]
tr\|A0A1S4DZZ0\|A0A1S4DZZ0_CUCME	3.6e-121	59.80	proline--tRNA ligase, cytoplasmic isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
tr\|A0A1S3BXN6\|A0A1S3BXN6_CUCME	3.6e-121	59.80	proline--tRNA ligase, cytoplasmic isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0000166	nucleotide binding
GO:0004827	proline-tRNA ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006433	prolyl-tRNA aminoacylation

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **INTERPRO**
Term	Definition
IPR017449	Pro-tRNA_synth_II
IPR004154	Anticodon-bd
IPR036621	Anticodon-bd_dom_sf
IPR016061	Pro-tRNA_ligase_II_C

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006525	arginine metabolic process
biological_process	GO:0006560	proline metabolic process
biological_process	GO:0006433	prolyl-tRNA aminoacylation
biological_process	GO:0015074	DNA integration
biological_process	GO:0090304	nucleic acid metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004827	proline-tRNA ligase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_5G018610.1	CsaV3_5G018610.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016061	Proline-tRNA ligase, class II, C-terminal	SMART	SM00946	ProRS_C_1_2	coord: 278..337 e-value: 6.7E-9 score: 45.6
IPR016061	Proline-tRNA ligase, class II, C-terminal	PFAM	PF09180	ProRS-C_1	coord: 283..337 e-value: 2.9E-16 score: 59.3
None	No IPR available	GENE3D	G3DSA:3.30.930.10		coord: 90..205 e-value: 2.2E-39 score: 137.4
None	No IPR available	PANTHER	PTHR43382	FAMILY NOT NAMED	coord: 3..25 coord: 285..337 coord: 97..285
None	No IPR available	SUPERFAMILY	SSF55681	Class II aaRS and biotin synthetases	coord: 97..201 coord: 4..22
None	No IPR available	SUPERFAMILY	SSF52954	Class II aaRS ABD-related	coord: 202..300
IPR036621	Anticodon-binding domain superfamily	GENE3D	G3DSA:3.40.50.800		coord: 209..307 e-value: 7.1E-28 score: 98.8
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 214..299 e-value: 3.2E-14 score: 52.7
IPR017449	Prolyl-tRNA synthetase, class II	SUPERFAMILY	SSF64586	C-terminal domain of ProRS	coord: 283..337

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_5G018610	CSPI05G02420	Wild cucumber (PI 183967)	cpicucB287
CsaV3_5G018610	CmaCh04G021770	Cucurbita maxima (Rimu)	cmacucB0864
CsaV3_5G018610	CmoCh04G022780	Cucurbita moschata (Rifu)	cmocucB0850
CsaV3_5G018610	Cp4.1LG01g18810	Cucurbita pepo (Zucchini)	cpecucB0522
CsaV3_5G018610	CsGy5G012480	Cucumber (Gy14) v2	cgybcucB232
CsaV3_5G018610	Carg17714	Silver-seed gourd	carcucB0515
CsaV3_5G018610	Cla005859	Watermelon (97103) v1	cucwmB434
CsaV3_5G018610	MELO3C005193	Melon (DHL92) v3.5.1	cucmeB361
CsaV3_5G018610	ClCG01G007170	Watermelon (Charleston Gray)	cucwcgB386
CsaV3_5G018610	Lsi09G007620	Bottle gourd (USVL1VR-Ls)	cuclsiB346
CsaV3_5G018610	MELO3C005193.2	Melon (DHL92) v3.6.1	cucmedB353
CsaV3_5G018610	Cla97C01G007580	Watermelon (97103) v2	cucwmbB352
CsaV3_5G018610	Bhi12G000907	Wax gourd	cucwgoB450

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_5G018610	Silver-seed gourd	carcucB0866
CsaV3_5G018610	Cucumber (Gy14) v1	cgycucB567
CsaV3_5G018610	Cucurbita maxima (Rimu)	cmacucB0356
CsaV3_5G018610	Cucurbita moschata (Rifu)	cmocucB0351
CsaV3_5G018610	Cucurbita pepo (Zucchini)	cpecucB0213