CsaV3_5G000260 (gene) Cucumber (Chinese Long) v3

NameCsaV3_5G000260
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
Descriptionglucan endo-1,3-beta-glucosidase
Locationchr5 : 119228 .. 120993 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTCTCCTTTCACTCTCTCACACCACCTTCTTCTTCCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCCCAACACTCTTCCAAATTCAAGGTAAATCAATTACATCATGCATTATTCTTATGTGCTTTTTTTCTTTTCAGTTTTCACTATCATATTTTTTCTTTATAAAAACATTTAACTTTCTTCAGGAGTTAATGGTGGATCAATCGGAGTCAATTACGGCACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCGACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAATGATCAAATACCCCGCTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACAAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATTGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCTCTCATTGAAGCCTCCTTGGATAGAAGGATTCAAATCTCAACGCCCCACTCGCTCGGCATTTTGTCGAACTCAACCCCGCCGTCGACTGCAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTAAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGACAATCTAGATTACGCGCTCTTCCGGCCGAACCCCGGCGTGTTCGACTCGGATTTAGGAATTCTATACACCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGCTTGGGCTTTGAGGATCTCGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGATCCAACCCAAGTCGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTGATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAGAATCTCAAACCGGGTCCAATTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGAGCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGTAGCTGCCACAGCTCGAACCCACCCTAAAACGTTTTTGATAATCATGATGGTTAGTGTTACATGTTGGTTGATAGGTTTGTTGTGGACTTGGATCTAACAGGCTCAATCCGCTACTCCGAGAGCCCATCAAGGTCAAGTTGAAGGCCCAAGCCCAAGCACAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAAGTGAGGCCAGCGAGGAGGGCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGACTGTGGGCCGATAAAAGAAAATGGGGCGTGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACCGGCACTTTAACCACGGTGGATCCAAGTAAGTCCCATAAGATTCTTATTTTTATTCTCATTCTCATTGATTTTTTTTTAATTAGTCAAAATTCATTGACTAATTCTGTTGTAATTTAATTATTCACAAAGGTATTTTCTTTAGCAATTTGTTTTTGTTTTAATTTTCCAGGTTATGGGAAGTGCAAATATTGGTAA

mRNA sequence

ATGGCTTCCTCTCCTTTCACTCTCTCACACCACCTTCTTCTTCCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCCCAACACTCTTCCAAATTCAAGGAGTTAATGGTGGATCAATCGGAGTCAATTACGGCACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCGACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAATGATCAAATACCCCGCTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACAAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATTGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCTCTCATTGAAGCCTCCTTGGATAGAAGGATTCAAATCTCAACGCCCCACTCGCTCGGCATTTTGTCGAACTCAACCCCGCCGTCGACTGCAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTAAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGACAATCTAGATTACGCGCTCTTCCGGCCGAACCCCGGCGTGTTCGACTCGGATTTAGGAATTCTATACACCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGCTTGGGCTTTGAGGATCTCGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGATCCAACCCAAGTCGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTGATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAGAATCTCAAACCGGGTCCAATTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGAGCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGTAGCTGCCACAGCTCGAACCCACCCTAAAACGTTTTTGATAATCATGATGGCTCAATCCGCTACTCCGAGAGCCCATCAAGGTCAAGTTGAAGGCCCAAGCCCAAGCACAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAAGTGAGGCCAGCGAGGAGGGCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGACTGTGGGCCGATAAAAGAAAATGGGGCGTGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACCGGCACTTTAACCACGGTGGATCCAAGTTATGGGAAGTGCAAATATTGGTAA

Coding sequence (CDS)

ATGGCTTCCTCTCCTTTCACTCTCTCACACCACCTTCTTCTTCCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCCCAACACTCTTCCAAATTCAAGGAGTTAATGGTGGATCAATCGGAGTCAATTACGGCACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCGACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAATGATCAAATACCCCGCTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACAAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATTGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCTCTCATTGAAGCCTCCTTGGATAGAAGGATTCAAATCTCAACGCCCCACTCGCTCGGCATTTTGTCGAACTCAACCCCGCCGTCGACTGCAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTAAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGACAATCTAGATTACGCGCTCTTCCGGCCGAACCCCGGCGTGTTCGACTCGGATTTAGGAATTCTATACACCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGCTTGGGCTTTGAGGATCTCGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGATCCAACCCAAGTCGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTGATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAGAATCTCAAACCGGGTCCAATTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGAGCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGTAGCTGCCACAGCTCGAACCCACCCTAAAACGTTTTTGATAATCATGATGGCTCAATCCGCTACTCCGAGAGCCCATCAAGGTCAAGTTGAAGGCCCAAGCCCAAGCACAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAAGTGAGGCCAGCGAGGAGGGCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGACTGTGGGCCGATAAAAGAAAATGGGGCGTGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACCGGCACTTTAACCACGGTGGATCCAAGTTATGGGAAGTGCAAATATTGGTAA

Protein sequence

MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKYW
BLAST of CsaV3_5G000260 vs. NCBI nr
Match: XP_011654592.1 (PREDICTED: glucan endo-1,3-beta-glucosidase isoform X1 [Cucumis sativus] >KGN49838.1 hypothetical protein Csa_5G139170 [Cucumis sativus])

HSP 1 Score: 957.6 bits (2474), Expect = 1.6e-275
Identity = 473/490 (96.53%), Postives = 473/490 (96.53%), Query Frame = 0

Query: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60
           MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA
Sbjct: 21  MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 80

Query: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120
           AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP
Sbjct: 81  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 140

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS
Sbjct: 141 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 200

Query: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240
           NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF
Sbjct: 201 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 260

Query: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300
           DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN
Sbjct: 261 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 320

Query: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR
Sbjct: 321 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 380

Query: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR 420
           PT                 AQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR
Sbjct: 381 PT-----------------AQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR 440

Query: 421 NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV 480
           NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV
Sbjct: 441 NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV 493

Query: 481 DPSYGKCKYW 491
           DPSYGKCKYW
Sbjct: 501 DPSYGKCKYW 493

BLAST of CsaV3_5G000260 vs. NCBI nr
Match: XP_008437641.1 (PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo])

HSP 1 Score: 907.9 bits (2345), Expect = 1.5e-260
Identity = 448/497 (90.14%), Postives = 460/497 (92.56%), Query Frame = 0

Query: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60
           MAS PFT SHHLLLPYFSTLPTFLLLILPTL QIQGVNGGSIGVNYGTVADNLPPPSKVA
Sbjct: 20  MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 79

Query: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120
           AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP
Sbjct: 80  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 139

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL+EASLDRRIQISTPHSLGILS
Sbjct: 140 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 199

Query: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240
           NSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTA+NLDYALFRPNPGVF
Sbjct: 200 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 259

Query: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300
           DSDLGILYTNMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+QVGVGPKEAAYYN
Sbjct: 260 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 319

Query: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGP+GERNFGLFEPDL+PVYEIGILR
Sbjct: 320 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 379

Query: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQGQVEG-------PSPSTVSESKRWCLPKSEA 420
           PT                 AQSATPRAHQG VEG       PS STVSESKRWC+PK+EA
Sbjct: 380 PT-----------------AQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEA 439

Query: 421 SEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQ 480
           S E LQRNIDYVCGLGLDCGPI+ENG C+APNTVR HAAYVMNAYFQATEG +FDCDFDQ
Sbjct: 440 SVEALQRNIDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQ 499

Query: 481 TGTLTTVDPSYGKCKYW 491
           TG LTTVDPSYGKC+YW
Sbjct: 500 TGALTTVDPSYGKCRYW 499

BLAST of CsaV3_5G000260 vs. NCBI nr
Match: XP_022958170.1 (glucan endo-1,3-beta-glucosidase [Cucurbita moschata])

HSP 1 Score: 810.4 bits (2092), Expect = 3.2e-231
Identity = 404/493 (81.95%), Postives = 433/493 (87.83%), Query Frame = 0

Query: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60
           MASS F LS  LL  Y S   T L+L+LPTL Q+Q   GGSIGVNYGT+ADNLP PSKVA
Sbjct: 1   MASS-FALS-RLLRAYTSIAATLLILLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVA 60

Query: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120
            FLLD+TIIDR+RLFDA+PDILRAFAHT ISV+IT+PNDQIPRLVKPNFAEEWIKFN+QP
Sbjct: 61  EFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQP 120

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180
           Y+PAT+IIR+LVGNEVLSTANK+LIANLVPAMQSLHTAL EASL+RRIQ+STPHSLGILS
Sbjct: 121 YVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNRRIQVSTPHSLGILS 180

Query: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240
           NS+PPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFF  TA+ LDYALFRPNPGV+
Sbjct: 181 NSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVY 240

Query: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300
           DSD GILYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDP Q+GVGPKEAA YN
Sbjct: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYN 300

Query: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360
           GNL+RHVVSG GTPLMPNRTFETYIFALFNENLKPGP+GERNFGLFEPDLSPVYEIGILR
Sbjct: 301 GNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILR 360

Query: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQG---QVEGPSPSTVSESKRWCLPKSEASEEG 420
           PT                 AQS+TPRAH G     +GPSPS V++ KRWCLPK+EA  E 
Sbjct: 361 PT-----------------AQSSTPRAHHGPEPTPKGPSPSPVADGKRWCLPKTEADSEA 420

Query: 421 LQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTL 480
           LQRNIDYVCGLGLDCGPI+ENG CF PNTVRAHAAYVMNAYFQAT+GND DCDF QTG L
Sbjct: 421 LQRNIDYVCGLGLDCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDL 473

Query: 481 TTVDPSYGKCKYW 491
           TTVDPSYGKCKYW
Sbjct: 481 TTVDPSYGKCKYW 473

BLAST of CsaV3_5G000260 vs. NCBI nr
Match: XP_022995909.1 (glucan endo-1,3-beta-glucosidase [Cucurbita maxima])

HSP 1 Score: 795.4 bits (2053), Expect = 1.1e-226
Identity = 397/493 (80.53%), Postives = 428/493 (86.82%), Query Frame = 0

Query: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60
           MASS F LS  LL  Y S   T LLL+LPTL Q+Q   GGSIGVNYGT+ADNLP PSKVA
Sbjct: 39  MASS-FALS-RLLRAYTSIAVTLLLLLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVA 98

Query: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120
            FLLD+TII R+RLFDA+PDILRAFAHT ISV+IT+PNDQIPRLVKPNFAEEWIKFN+QP
Sbjct: 99  EFLLDSTIIGRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQP 158

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180
           Y+PAT+IIR+LVGNEVLSTANK+LIANLVPAMQSLHTAL EASL+ RIQ+STPHSLGILS
Sbjct: 159 YVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNSRIQVSTPHSLGILS 218

Query: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240
           NS+PPSTARFRQGYDTHVIKPMLSFL+ETNSPLMVNPYPFF  TA+ LDYALFRPNPGV+
Sbjct: 219 NSSPPSTARFRQGYDTHVIKPMLSFLKETNSPLMVNPYPFFGTTAETLDYALFRPNPGVY 278

Query: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300
           DSD GILYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDP Q+GVGPKEAA YN
Sbjct: 279 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYN 338

Query: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360
           GNL+RHVVSG GTPLMPNRTFETYIFALFNE+LKPGP+GERNFGLFEPDL+PVYEIGILR
Sbjct: 339 GNLLRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLNPVYEIGILR 398

Query: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQG---QVEGPSPSTVSESKRWCLPKSEASEEG 420
           PT                 AQS+TPRAH G     +GPSPS V++ KRWCLPK+EA  E 
Sbjct: 399 PT-----------------AQSSTPRAHHGPEPTPKGPSPSPVADGKRWCLPKTEADSEA 458

Query: 421 LQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTL 480
           LQRNIDYVCGLG DCGPI+ NG CF PNTVRAHAAYVMNAYFQA +GND DCDF QTG L
Sbjct: 459 LQRNIDYVCGLGFDCGPIQANGPCFLPNTVRAHAAYVMNAYFQAIDGNDSDCDFQQTGDL 511

Query: 481 TTVDPSYGKCKYW 491
           TTVDPSYGKCKYW
Sbjct: 519 TTVDPSYGKCKYW 511

BLAST of CsaV3_5G000260 vs. NCBI nr
Match: XP_023521611.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793.9 bits (2049), Expect = 3.1e-226
Identity = 399/495 (80.61%), Postives = 424/495 (85.66%), Query Frame = 0

Query: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60
           MASS F LS  LL  Y S   T LLL+LPTL  IQ   GGSIGVNYGT+ADNLP PSKVA
Sbjct: 16  MASS-FALS-RLLRAYTSIAATLLLLLLPTLLHIQ-AKGGSIGVNYGTLADNLPLPSKVA 75

Query: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120
            FLLD+TIIDR+RLFDA+PDILRAFAHT ISV+IT+PNDQIPRLVKPNFAEEWIKFN+QP
Sbjct: 76  EFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQP 135

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180
           Y+PAT+IIR+LVGNEVLSTANK+LIANLVPAMQSLHTAL EASL RRIQ+STPHSLGILS
Sbjct: 136 YVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLSRRIQVSTPHSLGILS 195

Query: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240
           NS+PPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFF  TA+ LDYALFRPNPGV+
Sbjct: 196 NSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVY 255

Query: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300
           DSD GILYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDP Q+GVGPKEAA YN
Sbjct: 256 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYN 315

Query: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360
           GNL+RHVVSG GTPLMPNRTFETYIFALFNENLKPGP+GERNFGLFEPDLSPVYEIGILR
Sbjct: 316 GNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILR 375

Query: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQG-----QVEGPSPSTVSESKRWCLPKSEASE 420
           PT                 AQS+TPRAH G              V++ K WCLPK+EA  
Sbjct: 376 PT-----------------AQSSTPRAHHGPEXXXXXXXXXXXXVADGKTWCLPKTEADS 435

Query: 421 EGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTG 480
           E LQRNIDYVCGLGLDCGPI+ENG CF PNTVRAHAAYVMNAYFQAT+GND DCDF QTG
Sbjct: 436 EALQRNIDYVCGLGLDCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTG 490

Query: 481 TLTTVDPSYGKCKYW 491
            LTTVDPSYGKCKYW
Sbjct: 496 DLTTVDPSYGKCKYW 490

BLAST of CsaV3_5G000260 vs. TAIR10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 592.0 bits (1525), Expect = 3.3e-169
Identity = 288/477 (60.38%), Postives = 356/477 (74.63%), Query Frame = 0

Query: 13  LLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRV 72
           +LP+FS     +  I+  L  +   +  SIGVNYGT+A+NLPPP +VA FLL +T+I+R+
Sbjct: 4   VLPFFS---LSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRI 63

Query: 73  RLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLV 132
           RLFD DP IL+AFA+TGI+V++T+PNDQIP L   + A++WI  +IQP+ P+TNIIR+LV
Sbjct: 64  RLFDTDPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILV 123

Query: 133 GNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQ 192
           GNEV+STA+ LLI  L+PAMQSLHTAL+ ASL RRIQISTPHSLG L+NSTPPS+A+FR+
Sbjct: 124 GNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRR 183

Query: 193 GYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNML 252
           GYD  V+KP+LSFLR T+SP +VNPYPFF  + + LD+ALFRPNPG+FD    +LYTNML
Sbjct: 184 GYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNML 243

Query: 253 DAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKG 312
           DAQLD+VYSAM  LGF D++IVI E GWPS+GD  Q+GV    AA +N NL+  V SG G
Sbjct: 244 DAQLDSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTG 303

Query: 313 TPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPK 372
           TPLMPNRTFETYIFALFNENLK GP  ERNFG+F  DL+P+Y+IGILRPT  ++   +  
Sbjct: 304 TPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVY-- 363

Query: 373 TFLIIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDC 432
                       PR         SP   S SKRWC+ K+ A    LQRNIDYVCGLGLDC
Sbjct: 364 -----------NPR---------SPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDC 423

Query: 433 GPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
            PI E G C+ PNTV+AH+ Y MN Y+Q  E ++FDCDFD TG +TT+DPSYG C+Y
Sbjct: 424 RPINEGGLCYLPNTVKAHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of CsaV3_5G000260 vs. TAIR10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 426.8 bits (1096), Expect = 1.8e-119
Identity = 219/474 (46.20%), Postives = 300/474 (63.29%), Query Frame = 0

Query: 22  TFLLLILPTLFQIQGVNGGS-IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPD 81
           + L L+L  LF I   N  S IGVNYG ++DNLPPPS+ A  LL +T I +VRL++AD  
Sbjct: 4   SILFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAK-LLQSTSIQKVRLYNADSS 63

Query: 82  ILRAFAHTGISVSITIPNDQIPRLVKP-NFAEEWIKFNIQPYIPATNIIRVLVGNEVLST 141
           I+ +   TGI + I + N  +P +    N A +WI  N+ P+ PA+NII + VGNEVL +
Sbjct: 64  IITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLS 123

Query: 142 ANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVI 201
            +  L+  L+PAMQ++  AL   SL  +I++ST H++ +L NS PPS   F   Y    +
Sbjct: 124 NDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAG-L 183

Query: 202 KPMLSFLRETNSPLMVNPYPFFACTAD----NLDYALFRPNPGVFDSDLGILYTNMLDAQ 261
           K +L FL +T SP  +NPYPFFA  +D     L + LF+PNPG  DS+ GI Y NM DAQ
Sbjct: 184 KGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQ 243

Query: 262 LDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPL 321
           +DAV+SA+KS+GFE +++++AETGWPS GD  +VG   + A  YNGNL+ H+ S  GTPL
Sbjct: 244 VDAVHSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPL 303

Query: 322 MPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFL 381
           MP ++ +TYIFALF+ENLKPGP  E++FGLF+PDLS  Y+IG+ + T + T+        
Sbjct: 304 MPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTTSSQTS-------- 363

Query: 382 IIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPI 441
                            + P    V+ S  WC+PK +A++E LQ ++D+VCG G+DCGPI
Sbjct: 364 -----------------QSPQLGKVT-SMGWCVPKEDATQEQLQDSLDWVCGQGIDCGPI 423

Query: 442 KENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
              G CF PN V +H AY MN YFQ +  N  DCDF +T  +T+ +PSY  C Y
Sbjct: 424 MPGGVCFEPNNVASHTAYAMNLYFQKSPENPTDCDFSKTARITSENPSYSSCVY 449

BLAST of CsaV3_5G000260 vs. TAIR10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 416.8 bits (1070), Expect = 1.9e-116
Identity = 212/453 (46.80%), Postives = 285/453 (62.91%), Query Frame = 0

Query: 42  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQI 101
           IGVNYG VADNLPPPS+    LL +T I +VRL+ ADP I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 102 PRLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALI 161
           P L   PN A +WI  N+ P+ PA+ I+ + VGNE+L + +  L+  L+PAMQ++  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 162 EASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 221
             SL  +I++ST +S+ +L +S PPS+  F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 222 FACTAD----NLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 281
           FA  +D     L + LF PN G  DS  GI YTNM DAQ+DAV+SA+KS+GFE ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 282 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 341
           ETGW S+GD  +VG     A  YNGNL+ H+ S  GTPLMP +  +TYIFAL++ENLKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 342 PIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGPS 401
           P  ER FGLF+ DLS VY++G+ + + ++                              +
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSS-----------------------------QT 385

Query: 402 PSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMN 461
           PS    S  WC+PK  A+ E LQ ++D+ CG G+DCG I+  GACF PN V +HAAY MN
Sbjct: 386 PSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMN 445

Query: 462 AYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
            YFQ +     DCDF +T T+T+ +PSY  C Y
Sbjct: 446 MYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVY 447

BLAST of CsaV3_5G000260 vs. TAIR10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 401.7 bits (1031), Expect = 6.3e-112
Identity = 212/471 (45.01%), Postives = 296/471 (62.85%), Query Frame = 0

Query: 22  TFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDI 81
           + LLL+   +F        +IGVNYGT+  NLPPP++VA F+   T ID V++FD +PDI
Sbjct: 9   SLLLLLCAAVFLTIPAVISAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVKIFDVNPDI 68

Query: 82  LRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTAN 141
           LRAFA TGISV +T+PN  IP L     A  W+  NI P+ P T I  + VGNE+L T +
Sbjct: 69  LRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILLTGD 128

Query: 142 KLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSN--STPPSTARFRQGYDTHVI 201
             +I NL+PAM++L+ AL+ A + R ++++T HSL I++   +  PS+ RFR G+D  ++
Sbjct: 129 NNMINNLLPAMRNLNNALVRAGV-RDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKGIL 188

Query: 202 KPMLSFLRETNSPLMVNPYPFFACTADNLDYALFR-PNPGVFDSDLGILYTNMLDAQLDA 261
            P+L++ R T SP MVNPYP+F     N+++A+FR P   V D     +YTNM DA +D+
Sbjct: 189 APILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALMDS 248

Query: 262 VYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPN 321
            YSAMK+LG+ D++IV+ ETGWPS  D       P  AA++N N+++    G+GTPLMPN
Sbjct: 249 TYSAMKALGYGDVNIVVGETGWPSACDAP--WCSPANAAWFNLNIIKR-AQGQGTPLMPN 308

Query: 322 RTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFLIIM 381
           R FETYIF LFNE  KPGP  ERN+GLF  D SPVY++G+LR                  
Sbjct: 309 RRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLR------------------ 368

Query: 382 MAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKEN 441
                  +   G+   P+PST     +WC+ +S A+   LQ +I++VCG G+DC PI+  
Sbjct: 369 -----NGQGGGGRPALPAPSTA--GGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAG 428

Query: 442 GACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
           G+CF P+++R HA++VMNAYFQ+    D  C+F  TG +   +PS G CKY
Sbjct: 429 GSCFNPSSLRTHASFVMNAYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

BLAST of CsaV3_5G000260 vs. TAIR10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 365.5 bits (937), Expect = 5.0e-101
Identity = 192/479 (40.08%), Postives = 280/479 (58.46%), Query Frame = 0

Query: 18  STLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDA 77
           S LP FL+L   +       + G +GVNYG +A+NLP P KV   LL +  I+R+++FD 
Sbjct: 4   SFLPYFLIL---SFLSAIDAHSGMVGVNYGRIANNLPSPEKVVN-LLKSQGINRIKIFDT 63

Query: 78  DPDILRAFAHTGISVSITIPNDQIPRLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEV 137
           D ++L A A++ I V + +PN+ +       +FA+ WIK +I PY PAT I  + VGNEV
Sbjct: 64  DKNVLTALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEV 123

Query: 138 LSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDT 197
               +  +   LV AM+++HT+L++  LD+ I+IS+P +L  L+NS PPS+  F+     
Sbjct: 124 F--VDPTITPYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIE 183

Query: 198 HVIKPMLSFLRETNSPLMVNPYPFFACTAD----NLDYALFRPNPGVFDSDLGILYTNML 257
            V+KPML+ L++T+S LMVN YPFFA  A+    +LDYALF+ N G  DS  G+ Y ++ 
Sbjct: 184 PVVKPMLALLQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLF 243

Query: 258 DAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKG 317
           DAQ+DAVY+A+ ++GF+ + +++ ETGWPS GD  ++G     AA YN  L++ V++GKG
Sbjct: 244 DAQIDAVYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKG 303

Query: 318 TPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPK 377
           TPL P      Y+FALFNEN KPGP  ERN+GLF P+   VY +   +            
Sbjct: 304 TPLRPTEPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTK------------ 363

Query: 378 TFLIIMMAQSATP-RAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGL-GL 437
                   +S TP   ++G+V         E   WC+   E ++E LQ  +DY CG  G 
Sbjct: 364 --------KSTTPVNGNRGKV-----PVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGA 423

Query: 438 DCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
           DC PI+    C+ P ++ AHA+Y  N+Y+Q        C F     + T  P YGKC++
Sbjct: 424 DCRPIQPGATCYHPESLEAHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPPRYGKCEF 451

BLAST of CsaV3_5G000260 vs. Swiss-Prot
Match: sp|P52409|E13B_WHEAT (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 3.4e-123
Identity = 221/453 (48.79%), Postives = 295/453 (65.12%), Query Frame = 0

Query: 42  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQI 101
           IGVNYG  ADNLP P+ VA FL   T IDRV+LFDA+P  + AFA T IS+++++PN  +
Sbjct: 26  IGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVSLPNSAL 85

Query: 102 PRLVKP----NFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHT 161
           P L       + A  WI+ N+ PY+PATN+  +L GNE+L + +  LI +L+PAM+ L  
Sbjct: 86  PALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPAMRRLAQ 145

Query: 162 ALIEASLDRRIQISTPHSLGILSNSTP-PSTARFRQGYDTHVIKPMLSFLRETNSPLMVN 221
           AL    L   ++++TPH LGIL+ S   PS A FR GY+T +   ML F R+T SP MVN
Sbjct: 146 ALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGSPFMVN 205

Query: 222 PYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 281
           PYP+F+   + L+YALFRPN G++D    + YT+MLDAQ+DA+Y+AMK LG+ D+DI + 
Sbjct: 206 PYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLGYGDVDIAVG 265

Query: 282 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 341
           E GWP++ +P Q+GVG +EA  +N  ++R   SGKGTPLMPNRTFETY+F+LF+EN KPG
Sbjct: 266 EAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDENQKPG 325

Query: 342 PIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGPS 401
           PI ER+FGLF PD +PVY++G+LR                   A  A             
Sbjct: 326 PIAERHFGLFNPDFTPVYDLGLLRDG-----------------ASVAPXXXXXXXXXXXX 385

Query: 402 PSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMN 461
                   +WC+ K  A+   LQ NI+Y CG  +DC PI+  GACF+PN+++AHA+YVMN
Sbjct: 386 XXXXXXGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYVMN 445

Query: 462 AYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
           AY+QA    D  CDF  TG +T+ DPSYG CKY
Sbjct: 446 AYYQANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

BLAST of CsaV3_5G000260 vs. Swiss-Prot
Match: sp|Q9M069|E137_ARATH (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 416.8 bits (1070), Expect = 3.4e-115
Identity = 212/453 (46.80%), Postives = 285/453 (62.91%), Query Frame = 0

Query: 42  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQI 101
           IGVNYG VADNLPPPS+    LL +T I +VRL+ ADP I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 102 PRLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALI 161
           P L   PN A +WI  N+ P+ PA+ I+ + VGNE+L + +  L+  L+PAMQ++  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 162 EASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 221
             SL  +I++ST +S+ +L +S PPS+  F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 222 FACTAD----NLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 281
           FA  +D     L + LF PN G  DS  GI YTNM DAQ+DAV+SA+KS+GFE ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 282 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 341
           ETGW S+GD  +VG     A  YNGNL+ H+ S  GTPLMP +  +TYIFAL++ENLKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 342 PIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGPS 401
           P  ER FGLF+ DLS VY++G+ + + ++                              +
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSS-----------------------------QT 385

Query: 402 PSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMN 461
           PS    S  WC+PK  A+ E LQ ++D+ CG G+DCG I+  GACF PN V +HAAY MN
Sbjct: 386 PSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMN 445

Query: 462 AYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
            YFQ +     DCDF +T T+T+ +PSY  C Y
Sbjct: 446 MYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVY 447

BLAST of CsaV3_5G000260 vs. Swiss-Prot
Match: sp|Q94G86|ALL9_OLEEU (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.0e-111
Identity = 207/474 (43.67%), Postives = 296/474 (62.45%), Query Frame = 0

Query: 22  TFLLLILPTLFQIQGVNGGS-IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPD 81
           + L L+   L      N  S +GVNYG ++DNL P  +    LL +T I +VRLF A+P 
Sbjct: 9   SLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNL-PSLQATVNLLKSTTIQKVRLFGAEPA 68

Query: 82  ILRAFAHTGISVSITIPNDQIPRLV-KPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLST 141
           +++AFA+TG+ + I   N  IP L   PN A +++K N+  + PA+NII + VGNEVL++
Sbjct: 69  VIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTS 128

Query: 142 ANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVI 201
            ++ LI+ L+PAMQ++  AL  ASL  ++++ST H++ +LS S PPS+  F  G     +
Sbjct: 129 GDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLG-DTM 188

Query: 202 KPMLSFLRETNSPLMVNPYPFFAC----TADNLDYALFRPNPGVFDSDLGILYTNMLDAQ 261
           K +L F    ++P M++PYP+FA     T D L + LF+PN G  DS  G  YTNM DAQ
Sbjct: 189 KALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQ 248

Query: 262 LDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPL 321
           +DAV+SA+ ++GF+D++IV+AETGWP  GD  +VG     A  Y GNL+ H+ S  GTPL
Sbjct: 249 VDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPL 308

Query: 322 MPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFL 381
           MP ++ +TY+F+L++E+ K G   E+ FGLF+PD S  Y++G+L+ T   T    P    
Sbjct: 309 MPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATP---- 368

Query: 382 IIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPI 441
                 + TP+A               +  WC+PK   S++ L  NI+Y CG G+DCGPI
Sbjct: 369 ------TPTPKA---------------AGSWCVPKPGVSDDQLTGNINYACGQGIDCGPI 428

Query: 442 KENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
           +  GACF PNTV+AHAAYVMN Y+Q+   N ++CDF QT TLT  +PSYG C +
Sbjct: 429 QPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNF 455

BLAST of CsaV3_5G000260 vs. Swiss-Prot
Match: sp|Q9ZU91|E133_ARATH (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 9.3e-89
Identity = 186/476 (39.08%), Postives = 270/476 (56.72%), Query Frame = 0

Query: 24  LLLILPTLFQIQGVNGGS-IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDIL 83
           LLL+   LF    ++  S IGVN GT   N+P P++V A LL +  I+RVRL+DAD  +L
Sbjct: 4   LLLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVA-LLKSQNINRVRLYDADRSML 63

Query: 84  RAFAHTGISVSITIPNDQIPRLVKPN-FAEEWIKFNIQPYIPATNIIRVLVGNEVLSTAN 143
            AFAHTG+ V I++PNDQ+  + + N  A  W+  N+  Y PATNI  + VG+EVL++  
Sbjct: 64  LAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLT 123

Query: 144 KLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKP 203
               + LV A++ +  AL+ A+LDR+I++STPHS  I+ +S PPS A F + +D  VI P
Sbjct: 124 N-AASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDP-VIVP 183

Query: 204 MLSFLRETNSPLMVNPYPFFACTADN----LDYALFRP---NPGVFDSDLGILYTNMLDA 263
           +L FL+ T SPL++N YP+F     N    LDYALF+P   N    D++  + YTN+ DA
Sbjct: 184 LLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDA 243

Query: 264 QLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTP 323
            +DA Y AM  L F ++ IV+ E+GWPSKG P++     + A  YN NL++HV++  GTP
Sbjct: 244 IVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTP 303

Query: 324 LMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTF 383
             P     TYI+ L+NE+ +PGP+ E+N+GLF  + +PVY + +          T  +TF
Sbjct: 304 KHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTF 363

Query: 384 LIIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLG-LDCG 443
                                           C+ K +   + LQ  +D+ CG G +DC 
Sbjct: 364 --------------------------------CIAKEKVDRKMLQAALDWACGPGKVDCS 423

Query: 444 PIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
            + +  +C+ P+ V AH+ Y  NAY+Q        CDF    T+TT DPS G C +
Sbjct: 424 ALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVF 444

BLAST of CsaV3_5G000260 vs. Swiss-Prot
Match: sp|Q8VYE5|E1312_ARATH (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 7.9e-88
Identity = 172/454 (37.89%), Postives = 256/454 (56.39%), Query Frame = 0

Query: 42  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQI 101
           IG+ YG  ADNLP P++V+  L+ +  I  VR++DA+ D+L+AFA+TGI + I +PN  +
Sbjct: 27  IGICYGRNADNLPSPNRVSE-LIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADL 86

Query: 102 PRLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALI 161
               +  +  + W+  NI PY P+T I  + VG EV    +      ++PAM+++HTAL 
Sbjct: 87  LAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-ATGLVLPAMRNIHTALK 146

Query: 162 EASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 221
           ++ LD++I+IS+ HSL ILS S PPS+A F + +    +KPML FL E  SP M++ YP+
Sbjct: 147 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSA-FLKPMLEFLVENESPFMIDLYPY 206

Query: 222 FACTADN----LDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 281
           +A         L+YALF  +  V D   G+LY+NM DAQLDA+Y A+ ++ F+ + +++ 
Sbjct: 207 YAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 282 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 341
           E+GWPSKG P +    P+ A  YN NL+RHV+   GTP  P    + Y+F+LFNEN KPG
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 342 PIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGPS 401
              ERN+G+F  + + VY +          + T+  T                       
Sbjct: 327 IESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTT--XXXXXXXXXXXXXXXXXXXXX 386

Query: 402 PSTVSESKRWCLPKSEASEEGLQRNIDYVCGLG-LDCGPIKENGACFAPNTVRAHAAYVM 461
                    WC+  S+AS   LQ  +D+ CG G +DC  ++ +  CF P+TV +HA+Y  
Sbjct: 387 XXXXXXXXXWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAF 446

Query: 462 NAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
           N Y+Q +  +  DC F+        DPSYG C Y
Sbjct: 447 NTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of CsaV3_5G000260 vs. TrEMBL
Match: tr|A0A0A0KJS0|A0A0A0KJS0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 1.1e-275
Identity = 473/490 (96.53%), Postives = 473/490 (96.53%), Query Frame = 0

Query: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60
           MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA
Sbjct: 21  MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 80

Query: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120
           AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP
Sbjct: 81  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 140

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS
Sbjct: 141 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 200

Query: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240
           NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF
Sbjct: 201 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 260

Query: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300
           DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN
Sbjct: 261 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 320

Query: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR
Sbjct: 321 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 380

Query: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR 420
           PT                 AQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR
Sbjct: 381 PT-----------------AQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR 440

Query: 421 NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV 480
           NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV
Sbjct: 441 NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV 493

Query: 481 DPSYGKCKYW 491
           DPSYGKCKYW
Sbjct: 501 DPSYGKCKYW 493

BLAST of CsaV3_5G000260 vs. TrEMBL
Match: tr|A0A1S3AV36|A0A1S3AV36_CUCME (glucan endo-1,3-beta-glucosidase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 9.8e-261
Identity = 448/497 (90.14%), Postives = 460/497 (92.56%), Query Frame = 0

Query: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60
           MAS PFT SHHLLLPYFSTLPTFLLLILPTL QIQGVNGGSIGVNYGTVADNLPPPSKVA
Sbjct: 20  MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 79

Query: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120
           AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP
Sbjct: 80  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 139

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL+EASLDRRIQISTPHSLGILS
Sbjct: 140 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 199

Query: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240
           NSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTA+NLDYALFRPNPGVF
Sbjct: 200 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 259

Query: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300
           DSDLGILYTNMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+QVGVGPKEAAYYN
Sbjct: 260 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 319

Query: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGP+GERNFGLFEPDL+PVYEIGILR
Sbjct: 320 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 379

Query: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQGQVEG-------PSPSTVSESKRWCLPKSEA 420
           PT                 AQSATPRAHQG VEG       PS STVSESKRWC+PK+EA
Sbjct: 380 PT-----------------AQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEA 439

Query: 421 SEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQ 480
           S E LQRNIDYVCGLGLDCGPI+ENG C+APNTVR HAAYVMNAYFQATEG +FDCDFDQ
Sbjct: 440 SVEALQRNIDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQ 499

Query: 481 TGTLTTVDPSYGKCKYW 491
           TG LTTVDPSYGKC+YW
Sbjct: 500 TGALTTVDPSYGKCRYW 499

BLAST of CsaV3_5G000260 vs. TrEMBL
Match: tr|A0A1R3IZA5|A0A1R3IZA5_COCAP (Glycoside hydrolase, family 17 OS=Corchorus capsularis OX=210143 GN=CCACVL1_08667 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 3.6e-194
Identity = 326/474 (68.78%), Postives = 392/474 (82.70%), Query Frame = 0

Query: 16  YFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLF 75
           + S L T +L  L +    +GV GG+IGVNYGTVA+NLPPP++VA FLL++T+I+RVRLF
Sbjct: 9   FSSILVTLILFCLASSMLFRGV-GGTIGVNYGTVANNLPPPAEVAHFLLESTVINRVRLF 68

Query: 76  DADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNE 135
           DA+PDIL+AFAHTGI+V++T+PNDQIPRL K NFA++W++ NIQP+ PATNI+R+LVGNE
Sbjct: 69  DANPDILKAFAHTGIAVTVTVPNDQIPRLTKLNFAQQWVEDNIQPHTPATNIVRILVGNE 128

Query: 136 VLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYD 195
           V+STANKLLI +LVPAMQ+LHTAL+ ASLDRRIQ+STPHSLGILSNS+PPST +FRQGYD
Sbjct: 129 VISTANKLLIGSLVPAMQTLHTALVAASLDRRIQVSTPHSLGILSNSSPPSTGKFRQGYD 188

Query: 196 THVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQ 255
           THV+KP+LSFLR TNSP MVNPYPFF C+ + LDYALFRPN GVFD    +LYTNMLDAQ
Sbjct: 189 THVLKPLLSFLRATNSPFMVNPYPFFGCSPETLDYALFRPNAGVFDEITELLYTNMLDAQ 248

Query: 256 LDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPL 315
           LDAV+SAMK LGF+DL+IVIAETGWPS GD  QVGV  K AA YNGNLMRHV SG GTPL
Sbjct: 249 LDAVFSAMKFLGFDDLEIVIAETGWPSMGDSAQVGVDAKSAAEYNGNLMRHVTSGAGTPL 308

Query: 316 MPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFL 375
           M NRTFETYIFALFNENLKPGP  ERNFGLF PD++PVY++GILRPT  ++   +PK+  
Sbjct: 309 MRNRTFETYIFALFNENLKPGPTCERNFGLFRPDMTPVYDVGILRPTARSSIPNYPKS-- 368

Query: 376 IIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPI 435
                 ++ PR+ +G             K+WC+PK EA +  LQRNIDYVCGLGLDCGPI
Sbjct: 369 --PSPTNSAPRSSKG-------------KKWCVPKMEADDNALQRNIDYVCGLGLDCGPI 428

Query: 436 KENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
           +E+GACF PNTVRAHAA+ MNAY+Q++E  D+DCDF+QTG +T +DPSYGKC+Y
Sbjct: 429 QEHGACFLPNTVRAHAAFAMNAYYQSSEKKDYDCDFEQTGAITNIDPSYGKCQY 464

BLAST of CsaV3_5G000260 vs. TrEMBL
Match: tr|A0A061G2K4|A0A061G2K4_THECC (O-Glycosyl hydrolases family 17 protein OS=Theobroma cacao OX=3641 GN=TCM_015448 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 2.6e-192
Identity = 323/473 (68.29%), Postives = 391/473 (82.66%), Query Frame = 0

Query: 17  FSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFD 76
           FS++    +L       ++GV  G+IG+NYGTVA+NLPPP++VA FLL++T+I+RVRLFD
Sbjct: 26  FSSVLVAAILFFLASTPVRGVE-GTIGINYGTVANNLPPPAQVAHFLLESTVINRVRLFD 85

Query: 77  ADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEV 136
           A+PDIL+AFAHTGI++++T+PNDQIPRL K N A++W++ NIQPY PATNI+R+LVGNEV
Sbjct: 86  ANPDILKAFAHTGIAITVTVPNDQIPRLTKLNLAQQWVEDNIQPYTPATNIVRILVGNEV 145

Query: 137 LSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDT 196
           +STANKLLI +LVPA+Q+LHTAL+EASLDRRIQ+STPHSLGILSNS+PPST +FRQGYDT
Sbjct: 146 ISTANKLLIGSLVPAVQTLHTALVEASLDRRIQVSTPHSLGILSNSSPPSTGKFRQGYDT 205

Query: 197 HVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQL 256
           HV+KP+LSFLR TNSP MVNPYPFF C+ D LDYALFRPN GVFD +  + YTNMLDAQL
Sbjct: 206 HVLKPLLSFLRATNSPFMVNPYPFFGCSPDTLDYALFRPNAGVFDENTELHYTNMLDAQL 265

Query: 257 DAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLM 316
           DAV+SAMK +GF+DL+IVIAETGWPS GD TQVGV  + AA YNGNLMRHV SG GTPLM
Sbjct: 266 DAVFSAMKFVGFDDLEIVIAETGWPSMGDSTQVGVDAESAAEYNGNLMRHVASGAGTPLM 325

Query: 317 PNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFLI 376
           PNRTFETYIFALFNENLKPGP  ERNFGLF+PD++PVY++GILRPT  ++   +P     
Sbjct: 326 PNRTFETYIFALFNENLKPGPTCERNFGLFQPDMTPVYDVGILRPTARSSIPNNP----- 385

Query: 377 IMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIK 436
                  TP      V   +P+  SE K+WCLPK+ A E  LQRNIDYVCGLGLDCGPI+
Sbjct: 386 -------TPVPTAAPV---NPTPRSEGKQWCLPKTGADENALQRNIDYVCGLGLDCGPIQ 445

Query: 437 ENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 490
           E+GACF PNTVRAHAA+ MNAY+Q+T+  ++DC F+QTG +T VDPSYGKC+Y
Sbjct: 446 EDGACFLPNTVRAHAAFAMNAYYQSTDRKEYDCAFEQTGAITDVDPSYGKCQY 482

BLAST of CsaV3_5G000260 vs. TrEMBL
Match: tr|A0A1R3JFD6|A0A1R3JFD6_9ROSI (Glycoside hydrolase, family 17 OS=Corchorus olitorius OX=93759 GN=COLO4_16801 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 7.7e-189
Identity = 321/472 (68.01%), Postives = 388/472 (82.20%), Query Frame = 0

Query: 16  YFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLF 75
           + S L T +L  L +    +GV GG+IGVNYGTVA+NLPPP++VA FLL++T+I+RVRLF
Sbjct: 9   FSSILVTLILFCLASSTLFRGV-GGTIGVNYGTVANNLPPPAEVAHFLLESTVINRVRLF 68

Query: 76  DADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNE 135
           DA+PDIL+AFAHTGI+V++T+PNDQIPRL K NFA++W++ NIQP+ PATNI+R+LVGNE
Sbjct: 69  DANPDILKAFAHTGIAVTVTVPNDQIPRLTKLNFAQQWVEDNIQPHTPATNIVRILVGNE 128

Query: 136 VLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYD 195
           V+STANKLLI NLVPAMQ+LHTAL+ ASLDRRIQ+STPHSLGILSNS+PPST +FRQGYD
Sbjct: 129 VISTANKLLIGNLVPAMQTLHTALVAASLDRRIQVSTPHSLGILSNSSPPSTGKFRQGYD 188

Query: 196 THVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQ 255
           THV+KP+LSFLR TNSP MVNPYPFF C+ + LDYALFRPN GVFD    +LYTNMLDAQ
Sbjct: 189 THVLKPLLSFLRATNSPFMVNPYPFFGCSPETLDYALFRPNAGVFDEITELLYTNMLDAQ 248

Query: 256 LDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPL 315
           LDAV+SAMK LGF+DL+IVIAETGWPS GD  QVGV  K AA YNGNLMRHV SG GTPL
Sbjct: 249 LDAVFSAMKFLGFDDLEIVIAETGWPSMGDSAQVGVDAKSAAEYNGNLMRHVTSGAGTPL 308

Query: 316 MPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTFL 375
           M NRTFETYIFALFNENLKPGP  ERNFGLF PD++PVY++GILRPT  ++   +PK+  
Sbjct: 309 MRNRTFETYIFALFNENLKPGPTCERNFGLFRPDMTPVYDVGILRPTARSSIPNYPKS-- 368

Query: 376 IIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLD-CGP 435
                 ++ PR+ +G             K+WC+PK EA ++ LQRNIDYVCGLGL+ CGP
Sbjct: 369 --PSPTNSAPRSSKG-------------KKWCVPKMEADDDALQRNIDYVCGLGLEYCGP 428

Query: 436 IKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGK 487
           I+E+GACF PNTVRAHAA+ MNAY+Q++E  D+DCDF+QTG +T +DP+  K
Sbjct: 429 IQEDGACFLPNTVRAHAAFAMNAYYQSSEKKDYDCDFEQTGAITNIDPTTAK 462

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011654592.11.6e-27596.53PREDICTED: glucan endo-1,3-beta-glucosidase isoform X1 [Cucumis sativus] >KGN498... [more]
XP_008437641.11.5e-26090.14PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo][more]
XP_022958170.13.2e-23181.95glucan endo-1,3-beta-glucosidase [Cucurbita moschata][more]
XP_022995909.11.1e-22680.53glucan endo-1,3-beta-glucosidase [Cucurbita maxima][more]
XP_023521611.13.1e-22680.61glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G24318.13.3e-16960.38O-Glycosyl hydrolases family 17 protein[more]
AT2G16230.11.8e-11946.20O-Glycosyl hydrolases family 17 protein[more]
AT4G34480.11.9e-11646.80O-Glycosyl hydrolases family 17 protein[more]
AT3G55430.16.3e-11245.01O-Glycosyl hydrolases family 17 protein[more]
AT4G26830.15.0e-10140.08O-Glycosyl hydrolases family 17 protein[more]
Match NameE-valueIdentityDescription
sp|P52409|E13B_WHEAT3.4e-12348.79Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
sp|Q9M069|E137_ARATH3.4e-11546.80Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
sp|Q94G86|ALL9_OLEEU1.0e-11143.67Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
sp|Q9ZU91|E133_ARATH9.3e-8939.08Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
sp|Q8VYE5|E1312_ARATH7.9e-8837.89Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KJS0|A0A0A0KJS0_CUCSA1.1e-27596.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3 SV=1[more]
tr|A0A1S3AV36|A0A1S3AV36_CUCME9.8e-26190.14glucan endo-1,3-beta-glucosidase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV... [more]
tr|A0A1R3IZA5|A0A1R3IZA5_COCAP3.6e-19468.78Glycoside hydrolase, family 17 OS=Corchorus capsularis OX=210143 GN=CCACVL1_0866... [more]
tr|A0A061G2K4|A0A061G2K4_THECC2.6e-19268.29O-Glycosyl hydrolases family 17 protein OS=Theobroma cacao OX=3641 GN=TCM_015448... [more]
tr|A0A1R3JFD6|A0A1R3JFD6_9ROSI7.7e-18968.01Glycoside hydrolase, family 17 OS=Corchorus olitorius OX=93759 GN=COLO4_16801 PE... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR017853Glycoside_hydrolase_SF
IPR000490Glyco_hydro_17
IPR012946X8
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0052546 cell wall pectin metabolic process
biological_process GO:0052541 plant-type cell wall cellulose metabolic process
biological_process GO:0006606 protein import into nucleus
cellular_component GO:0005575 cellular_component
cellular_component GO:0009507 chloroplast
cellular_component GO:0005634 nucleus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0008565 protein transporter activity
molecular_function GO:0008536 Ran GTPase binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G000260.1CsaV3_5G000260.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 405..489
e-value: 1.8E-40
score: 150.4
IPR012946X8 domainPFAMPF07983X8coord: 406..476
e-value: 1.6E-18
score: 67.0
NoneNo IPR availableGENE3DG3DSA:3.20.20.80coord: 40..362
e-value: 1.7E-122
score: 410.3
NoneNo IPR availablePANTHERPTHR32227:SF62GLUCOSIDASE-LIKE PROTEINcoord: 23..489
NoneNo IPR availablePANTHERPTHR32227FAMILY NOT NAMEDcoord: 23..489
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 42..356
e-value: 3.4E-82
score: 276.2
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 271..284
IPR017853Glycoside hydrolase superfamilySUPERFAMILYSSF51445(Trans)glycosidasescoord: 42..357

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsaV3_5G000260Bottle gourd (USVL1VR-Ls)cuclsiB401
CsaV3_5G000260Bottle gourd (USVL1VR-Ls)cuclsiB402
CsaV3_5G000260Melon (DHL92) v3.5.1cucmeB411
CsaV3_5G000260Melon (DHL92) v3.5.1cucmeB416
CsaV3_5G000260Melon (DHL92) v3.6.1cucmedB349
CsaV3_5G000260Melon (DHL92) v3.6.1cucmedB404
CsaV3_5G000260Melon (DHL92) v3.6.1cucmedB410
CsaV3_5G000260Watermelon (Charleston Gray)cucwcgB385
CsaV3_5G000260Watermelon (Charleston Gray)cucwcgB413
CsaV3_5G000260Watermelon (Charleston Gray)cucwcgB417
CsaV3_5G000260Watermelon (97103) v1cucwmB423
CsaV3_5G000260Watermelon (97103) v1cucwmB433
CsaV3_5G000260Watermelon (97103) v1cucwmB430
CsaV3_5G000260Watermelon (97103) v2cucwmbB351
CsaV3_5G000260Watermelon (97103) v2cucwmbB395
CsaV3_5G000260Watermelon (97103) v2cucwmbB399
CsaV3_5G000260Wax gourdcucwgoB464
CsaV3_5G000260Wax gourdcucwgoB493
CsaV3_5G000260Cucumber (Chinese Long) v3cuccucB119
CsaV3_5G000260Cucumber (Chinese Long) v3cuccucB123
CsaV3_5G000260Silver-seed gourdcarcucB0541
CsaV3_5G000260Silver-seed gourdcarcucB0577
CsaV3_5G000260Silver-seed gourdcarcucB0611
CsaV3_5G000260Silver-seed gourdcarcucB1035
CsaV3_5G000260Cucumber (Gy14) v2cgybcucB130
CsaV3_5G000260Cucumber (Gy14) v2cgybcucB133
CsaV3_5G000260Cucumber (Gy14) v1cgycucB007
CsaV3_5G000260Cucumber (Gy14) v1cgycucB393
CsaV3_5G000260Cucurbita maxima (Rimu)cmacucB0305
CsaV3_5G000260Cucurbita maxima (Rimu)cmacucB0342
CsaV3_5G000260Cucurbita maxima (Rimu)cmacucB0402
CsaV3_5G000260Cucurbita maxima (Rimu)cmacucB0500
CsaV3_5G000260Cucurbita maxima (Rimu)cmacucB0735
CsaV3_5G000260Cucurbita moschata (Rifu)cmocucB0296
CsaV3_5G000260Cucurbita moschata (Rifu)cmocucB0338
CsaV3_5G000260Cucurbita moschata (Rifu)cmocucB0398
CsaV3_5G000260Cucurbita moschata (Rifu)cmocucB0490
CsaV3_5G000260Cucurbita moschata (Rifu)cmocucB0722
CsaV3_5G000260Cucurbita moschata (Rifu)cmocucB0723
CsaV3_5G000260Cucurbita moschata (Rifu)cmocucB0976
CsaV3_5G000260Cucurbita pepo (Zucchini)cpecucB0046
CsaV3_5G000260Cucurbita pepo (Zucchini)cpecucB0226
CsaV3_5G000260Cucurbita pepo (Zucchini)cpecucB0275
CsaV3_5G000260Cucurbita pepo (Zucchini)cpecucB0774
CsaV3_5G000260Cucurbita pepo (Zucchini)cpecucB0910
CsaV3_5G000260Cucurbita pepo (Zucchini)cpecucB1071
CsaV3_5G000260Wild cucumber (PI 183967)cpicucB163
CsaV3_5G000260Wild cucumber (PI 183967)cpicucB166
CsaV3_5G000260Bottle gourd (USVL1VR-Ls)cuclsiB345