CsaV3_3G036550 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_3G036550
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
DescriptionS-RNase
Locationchr3 : 30347410 .. 30348151 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTCAATCTATTTTAATTATTTACTTTTTGAGTTTGGTGGTGTTCGTTAATGGCGGCGTTCCATTTGAGTTCTTCCAATTCGTGGAACAGTGGGGGCCGAATGTATGTAATACTGGCGCTCGTGTCTCTCCATGTCATACTCCGCCTCAAGCCAAGTTCACTATTCATGGCTTGTGGCCAAGTAATCACAGTAATGCTCATCTTCTTTGTCAATCAATAACGAAATATACCAAGGTTTCCATCTTCTTTAATATTTGTATGTGTTCTTTTTAATCTATCTTTAACATTTTAACTAATATAACGTTGTAACATGCTTTAATTTTAGGTTTTATTACTTGAAAGACAACTGAATATATCTTGGCCAGATTTAAAAGGTGGGATGAACAATGAATTTTGGACTCATGAATGGAATTTTCATGGAAAATGTTCAGATCCTCCTTTTAGCTTATTTCAATATTTTCAAATGTCTTTGAATCTTCTACAAAAATTTGATGTGTTGGCCATCTTAAAGGCTGCCGGGCTCAACCCTCAAACCTCCCAAAACATTGGAATCCAAAACATCATGACCCCTATTCAAAGGACAACCAAAAAGTATCCTGGCATCCGCTGCTACAAAAATGTTAAAACGGGCAAGTCGCAGTTGAATGAAATTGTACTGTGTTTGGAAAAAGATGGTGCTACCCTCATCGACTGCCCTACATTTGTTAGTAGCACTTGTGCAAAATCAATTGTATGGTAG

mRNA sequence

ATGACTCAATCTATTTTAATTATTTACTTTTTGAGTTTGGTGGTGTTCGTTAATGGCGGCGTTCCATTTGAGTTCTTCCAATTCGTGGAACAGTGGGGGCCGAATGTATGTAATACTGGCGCTCGTGTCTCTCCATGTCATACTCCGCCTCAAGCCAAGTTCACTATTCATGGCTTGTGGCCAAGTAATCACAGTAATGCTCATCTTCTTTGTCAATCAATAACGAAATATACCAAGGTTTTATTACTTGAAAGACAACTGAATATATCTTGGCCAGATTTAAAAGGTGGGATGAACAATGAATTTTGGACTCATGAATGGAATTTTCATGGAAAATGTTCAGATCCTCCTTTTAGCTTATTTCAATATTTTCAAATGTCTTTGAATCTTCTACAAAAATTTGATGTGTTGGCCATCTTAAAGGCTGCCGGGCTCAACCCTCAAACCTCCCAAAACATTGGAATCCAAAACATCATGACCCCTATTCAAAGGACAACCAAAAAGTATCCTGGCATCCGCTGCTACAAAAATGTTAAAACGGGCAAGTCGCAGTTGAATGAAATTGTACTGTGTTTGGAAAAAGATGGTGCTACCCTCATCGACTGCCCTACATTTGTTAGTAGCACTTGTGCAAAATCAATTGTATGGTAG

Coding sequence (CDS)

ATGACTCAATCTATTTTAATTATTTACTTTTTGAGTTTGGTGGTGTTCGTTAATGGCGGCGTTCCATTTGAGTTCTTCCAATTCGTGGAACAGTGGGGGCCGAATGTATGTAATACTGGCGCTCGTGTCTCTCCATGTCATACTCCGCCTCAAGCCAAGTTCACTATTCATGGCTTGTGGCCAAGTAATCACAGTAATGCTCATCTTCTTTGTCAATCAATAACGAAATATACCAAGGTTTTATTACTTGAAAGACAACTGAATATATCTTGGCCAGATTTAAAAGGTGGGATGAACAATGAATTTTGGACTCATGAATGGAATTTTCATGGAAAATGTTCAGATCCTCCTTTTAGCTTATTTCAATATTTTCAAATGTCTTTGAATCTTCTACAAAAATTTGATGTGTTGGCCATCTTAAAGGCTGCCGGGCTCAACCCTCAAACCTCCCAAAACATTGGAATCCAAAACATCATGACCCCTATTCAAAGGACAACCAAAAAGTATCCTGGCATCCGCTGCTACAAAAATGTTAAAACGGGCAAGTCGCAGTTGAATGAAATTGTACTGTGTTTGGAAAAAGATGGTGCTACCCTCATCGACTGCCCTACATTTGTTAGTAGCACTTGTGCAAAATCAATTGTATGGTAG

Protein sequence

MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLCQSITKYTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPFSLFQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSIVW
Homology
[Close]
BLAST of CsaV3_3G036550 vs. NCBI nr
Match: KGN59020.1 (hypothetical protein Csa_3G745530 [Cucumis sativus])

HSP 1 Score: 410.6 bits (1054), Expect = 3.3e-111
Identity = 193/216 (89.35%), Postives = 203/216 (93.98%), Query Frame = 0 

Query: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60
           MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNHSNAHLLCQSITKYTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPFSL 120
           PSNHSNAHLLCQS T Y+KV+ LE QLNISWPDLK G N++FW HEW+ HGKCSDPPFSL
Sbjct: 61  PSNHSNAHLLCQSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSL 120

Query: 121 FQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKT 180
           FQYFQ+SLNLL+KFD+L ILKAAGLNPQTSQN+ IQNIM PIQRTTKKYPGIRC KNVKT
Sbjct: 121 FQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKT 180

Query: 181 GKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSIVW 217
           GKSQLNEIVLCLEKDGATLIDCPTFVS+TCAKS VW
Sbjct: 181 GKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]
BLAST of CsaV3_3G036550 vs. NCBI nr
Match: XP_011651927.1 (PREDICTED: ribonuclease MC-like [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_3G736680 [Cucumis sativus])

HSP 1 Score: 217.2 bits (552), Expect = 5.3e-53
Identity = 111/223 (49.78%), Postives = 140/223 (62.78%), Query Frame = 0 

Query: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60
           M Q I I+  LS ++FVN G+PF+FFQ V+QWGPN+C+     S C+  PQ  FTIHGLW
Sbjct: 1   MAQHIFILCLLSSLLFVN-GIPFDFFQLVQQWGPNICSPAG--SKCYAQPQPMFTIHGLW 60

Query: 61  PSNHSNAHLLCQSITK---YTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPP 120
           PSN S   +LC    K     KV  LE+ LN  WPD+  G N+ FW HEW  H  C DPP
Sbjct: 61  PSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPP 120

Query: 121 FSLFQYFQMSL--NLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCY 180
           F++F YF +SL   + + +D+L IL  AGL+ QT +++  QN++ PI+  T   PGIRC 
Sbjct: 121 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 180

Query: 181 KNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAK--SIVW 217
            N  T K QL EIVLC   DG TLIDCP+FVS+TC      VW
Sbjct: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVW 220

[Close]
BLAST of CsaV3_3G036550 vs. NCBI nr
Match: XP_022934593.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 189.1 bits (479), Expect = 1.6e-44
Identity = 100/217 (46.08%), Postives = 131/217 (60.37%), Query Frame = 0 

Query: 4   SILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSN 63
           SI    F+ L++ VN    F++FQ V+QW P  C++  +V  CH  P   FTIHGLWPSN
Sbjct: 9   SIFNFVFVVLILCVN--AQFDYFQMVQQWPPAKCSS-VKVR-CHVQPPTMFTIHGLWPSN 68

Query: 64  HSNAHLLC-QSITKYTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPFSLFQ 123
           +SNA L+C       T++  L  QLN  WPD+  G N +FW HEW+ HG CS+PPF+  Q
Sbjct: 69  YSNARLVCAPQPFNPTQIAALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQ 128

Query: 124 YFQMSLNLL--QKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKT 183
           YFQ++LN+     +D+L IL AAGL P  +     ++I   IQ  T K PG+RC  N ++
Sbjct: 129 YFQITLNIRTNHSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQS 188

Query: 184 -GKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSIVW 217
             K QL EI+LC +KDG TLIDC  F   TC    VW
Sbjct: 189 KEKKQLLEIILCFDKDGITLIDCTEFAGITCPPQFVW 221

[Close]
BLAST of CsaV3_3G036550 vs. NCBI nr
Match: XP_022932791.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 188.7 bits (478), Expect = 2.0e-44
Identity = 98/220 (44.55%), Postives = 125/220 (56.82%), Query Frame = 0 

Query: 1   MTQSILIIYFLSLVVFVN-GGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGL 60
           M Q+  I+     V+  +     F++FQ V+QW P  C +G R   C+  P A FTIHGL
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATC-SGVR---CYANPPAMFTIHGL 60

Query: 61  WPSNHSNAHLLCQSIT-KYTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPF 120
           WPSN+SN  L+C        ++  L  QLN  WPD+  G N  FW HEW+ HG CSDPPF
Sbjct: 61  WPSNYSNVPLVCAGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPF 120

Query: 121 SLFQYFQMSLNLL--QKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYK 180
           +  QYFQ +LN+     +D+LAIL  AGL P  +       I   IQ  T K PG+RC K
Sbjct: 121 NQLQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNK 180

Query: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSIVW 217
           N ++ K QL EI+LC +K+G TLIDC  F   TC    VW
Sbjct: 181 NAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVW 216

[Close]
BLAST of CsaV3_3G036550 vs. NCBI nr
Match: XP_023514499.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 188.3 bits (477), Expect = 2.7e-44
Identity = 95/196 (48.47%), Postives = 116/196 (59.18%), Query Frame = 0 

Query: 23  FEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLCQSITKYTKVLL 82
           F++FQ V+QW P  C +G R   C+  P A FTIHGLWPSN+SN  L+C   T       
Sbjct: 24  FDYFQMVQQWPPATC-SGVR---CYANPPAMFTIHGLWPSNYSNVPLIC---TGQPFNPT 83

Query: 83  LERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPFSLFQYFQMSLNLL--QKFDVLAIL 142
           L  QLN  WPD+  G N  FW HEW+ HG CSDPPF+  QYFQ +LN+     +D+LAIL
Sbjct: 84  LVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAIL 143

Query: 143 KAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKTGKSQLNEIVLCLEKDGATLI 202
             AGL P  +       I   IQ  T K PG+RC KN ++ K QL EI+LC +K+G TLI
Sbjct: 144 NTAGLGPTGTNTRQYGAIEGAIQAATGKKPGLRCNKNAQSKKKQLFEIILCFDKNGVTLI 203

Query: 203 DCPTFVSSTCAKSIVW 217
           DC  F   TC    VW
Sbjct: 204 DCTQFAGITCPSQFVW 212

[Close]
BLAST of CsaV3_3G036550 vs. TAIR10
Match: AT2G02990.1 (ribonuclease 1)

HSP 1 Score: 120.6 bits (301), Expect = 1.2e-27
Identity = 69/215 (32.09%), Postives = 108/215 (50.23%), Query Frame = 0 

Query: 6   LIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVS-PCHTPPQAKFTIHGLWPSNH 65
           L++   S+    +    F+FF FV+QW  + C+T  +   P    P A F IHGLWP+  
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYK 72

Query: 66  SNAH-LLCQSITKYTKVLL--LERQLNISWPDL--KGGMNNEFWTHEWNFHGKCSDPPFS 125
              +   C +   +    +  L   +  SWP L    G    FW HEW  HG CS+    
Sbjct: 73  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 132

Query: 126 LFQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVK 185
             +YFQ +LNL QK ++L  L  AG+NP   ++  +++I   I+ +    P + C ++  
Sbjct: 133 QHEYFQTALNLKQKTNLLGALTKAGINPD-GKSYSLESIRDSIKESIGFTPWVECNRD-G 192

Query: 186 TGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSI 215
           +G SQL ++ LC+++ G+ LI+CP F    C   I
Sbjct: 193 SGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEI 225

[Close]
BLAST of CsaV3_3G036550 vs. TAIR10
Match: AT1G14220.1 (Ribonuclease T2 family protein)

HSP 1 Score: 111.3 bits (277), Expect = 7.5e-25
Identity = 67/198 (33.84%), Postives = 98/198 (49.49%), Query Frame = 0 

Query: 23  FEFFQFVEQWGPNVCNTG-ARVSPCHTPPQAKFTIHGLWPS-NHSNAHLLCQSITKY--T 82
           F+FF FV QW    C+T  A   P    P A F IHGLWP+ N  +    C   +++  +
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQFDRS 87

Query: 83  KVLLLERQLNISWPDLKGGMNN--EFWTHEWNFHGKCSDPPFSLFQYFQMSLNLLQKFDV 142
           ++  L   L  +WP L    N    FW HEW  HG CS+      +YF+ +L L QK ++
Sbjct: 88  QISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLKQKANL 147

Query: 143 LAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKTGKSQLNEIVLCLEKDG 202
           L ILK +G+NP       +  I   I+      PGI C K+ +   +QL++I +C++  G
Sbjct: 148 LQILKNSGINPDDG-FYNLDKITNAIKDGIGFTPGIECNKDPER-NAQLHQIYICVDTSG 207

Query: 203 ATLIDCPTFVSSTCAKSI 215
              I+CP     +C   I
Sbjct: 208 TEFIECPVLPRGSCPSQI 223

[Close]
BLAST of CsaV3_3G036550 vs. TAIR10
Match: AT1G26820.1 (ribonuclease 3)

HSP 1 Score: 101.7 bits (252), Expect = 5.9e-22
Identity = 65/196 (33.16%), Postives = 96/196 (48.98%), Query Frame = 0 

Query: 23  FEFFQFVEQWGPNVCNTGARVSPCHT---PPQAKFTIHGLWPSNHSNA-HLLCQSITKY- 82
           F+FF FV QW    C+  +R S C+     P A F IHGLWP+  +      C   +++ 
Sbjct: 22  FDFFYFVLQWPGAYCD--SRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFD 81

Query: 83  -TKVLLLERQLNISWPDLKGGMNN--EFWTHEWNFHGKCSDPPFSLFQYFQMSLNLLQKF 142
             +V  L   L   WP L    N+  +FWTHEW  HG C++       YF+  L L QK 
Sbjct: 82  DLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKA 141

Query: 143 DVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKTGKSQLNEIVLCLEK 202
           ++L  L  AG+ P   +   +++I   I++     PGI C K+  +  SQL +I LC++ 
Sbjct: 142 NLLHALTNAGIKPD-DKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNSQLYQIYLCVDT 201

Query: 203 DGATLIDCPTFVSSTC 211
             +  I+CP      C
Sbjct: 202 SASKFINCPVMPHGRC 213

[Close]
BLAST of CsaV3_3G036550 vs. TAIR10
Match: AT2G39780.1 (ribonuclease 2)

HSP 1 Score: 80.9 bits (198), Expect = 1.1e-15
Identity = 59/192 (30.73%), Postives = 80/192 (41.67%), Query Frame = 0 

Query: 23  FEFFQFVEQWGPNVC---------NTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLC-- 82
           F++F    QW    C         N   R S   T    +FTIHGLWP  +  +   C  
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT----QFTIHGLWPDYNDGSWPSCCY 91

Query: 83  QSITKYTKVLLLERQLNISWPDLK-------GGMNNEFWTHEWNFHGKCSDPPF-SLFQY 142
           +S  K  ++  L   L   WP L         G    FW HEW  HG CS P F   + Y
Sbjct: 92  RSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNY 151

Query: 143 FQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKTGKS 196
           F  +LNL  K +V  +L  AG     S+   +  I+T IQ      P + C ++      
Sbjct: 152 FLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA----- 211

[Close]
BLAST of CsaV3_3G036550 vs. Swiss-Prot
Match: sp|P23540|RNMC_MOMCH (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.8e-29
Identity = 67/198 (33.84%), Postives = 107/198 (54.04%), Query Frame = 0 

Query: 23  FEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLCQSITKY--TKV 82
           F+ F FV+QW P VC+   +   C       FTIHGLWP     +   C   + +  TK+
Sbjct: 1   FDSFWFVQQWPPAVCSF-QKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKI 60

Query: 83  LLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPFSLFQYFQMSLNLLQKFDVLAIL 142
             L+ QLN  WP++    N +FW+HEW  HG CS+  F+   YF++++++   +D++  L
Sbjct: 61  SHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 143 K--AAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKTGKSQLNEIVLCLEKDGAT 202
           +  AAG N +T     I+     ++    K+PG+RC  + +T  S L ++V C  +DG+T
Sbjct: 121 RPHAAGPNGRTKSRQAIKGF---LKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGST 180

Query: 203 LIDCPTFVSSTCAKSIVW 217
           LIDC      TC  + ++
Sbjct: 181 LIDC---TRDTCGANFIF 191

[Close]
BLAST of CsaV3_3G036550 vs. Swiss-Prot
Match: sp|P42813|RNS1_ARATH (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.2e-26
Identity = 69/215 (32.09%), Postives = 108/215 (50.23%), Query Frame = 0 

Query: 6   LIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVS-PCHTPPQAKFTIHGLWPSNH 65
           L++   S+    +    F+FF FV+QW  + C+T  +   P    P A F IHGLWP+  
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYK 72

Query: 66  SNAH-LLCQSITKYTKVLL--LERQLNISWPDL--KGGMNNEFWTHEWNFHGKCSDPPFS 125
              +   C +   +    +  L   +  SWP L    G    FW HEW  HG CS+    
Sbjct: 73  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 132

Query: 126 LFQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVK 185
             +YFQ +LNL QK ++L  L  AG+NP   ++  +++I   I+ +    P + C ++  
Sbjct: 133 QHEYFQTALNLKQKTNLLGALTKAGINPD-GKSYSLESIRDSIKESIGFTPWVECNRD-G 192

Query: 186 TGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSI 215
           +G SQL ++ LC+++ G+ LI+CP F    C   I
Sbjct: 193 SGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEI 225

[Close]
BLAST of CsaV3_3G036550 vs. Swiss-Prot
Match: sp|P80022|RNLE_SOLLC (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 3.2e-25
Identity = 66/217 (30.41%), Postives = 115/217 (53.00%), Query Frame = 0 

Query: 5   ILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVS-PCHTPPQAKFTIHGLWPSN 64
           ++++     +  +N    F+FF FV+QW  + C+T      P    P A F IHGLWP+N
Sbjct: 11  LILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNN 70

Query: 65  HSNAH-LLCQSITKY--TKVLLLERQLNISWPDL--KGGMNNEFWTHEWNFHGKCSDPPF 124
           +   +   C   + Y  +++  L   +  +WP L    G  + FW+HEW  HG C++   
Sbjct: 71  NDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVL 130

Query: 125 -SLFQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKN 184
            +   YF+ +L+L  + D+L+IL+ A ++P   ++  + NI   I+      P I+C  +
Sbjct: 131 TNQHAYFKKALDLKNQIDLLSILQGADIHPD-GESYDLVNIRNAIKSAIGYTPWIQCNVD 190

Query: 185 VKTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSI 215
            ++G SQL ++ +C++  G++LI+CP F    C  SI
Sbjct: 191 -QSGNSQLYQVYICVDGSGSSLIECPIFPGGKCGTSI 225

[Close]
BLAST of CsaV3_3G036550 vs. Swiss-Prot
Match: sp|Q38717|RNS4_ANTHI (Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 6.1e-24
Identity = 61/187 (32.62%), Postives = 95/187 (50.80%), Query Frame = 0 

Query: 24  EFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHS-NAHLLC--QSITKYTKV 83
           ++ + V QW  + C   +R      P  + FTIHGLWP N++  A   C   +  ++T  
Sbjct: 34  DYLKLVLQWPKSFCLINSRKCQ-RNPLPSNFTIHGLWPDNYTRQAPQSCTTNNFQRFTDT 93

Query: 84  LLLERQLNISWPDLK----GGMNNEFWTHEWNFHGKCSDPPFSLFQYFQMSLNLLQKFDV 143
             +E+++  SWPDLK     G++  FW  +W  HG C  PP     YF  +L L  + DV
Sbjct: 94  -DIEQRMEESWPDLKQQSIAGLSYNFWQDQWRKHGSCCFPPHESEIYFLKALELKDRLDV 153

Query: 144 LAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKTGKSQLNEIVLCLEKDG 203
           L IL+    NP T Q   +  +   I R   K P ++C       +S L E+V+C++ +G
Sbjct: 154 LTILENNNFNPGTPQPFSVLRVFNTISRAIGKTPILKC------AQSYLKEVVICVDNNG 212

[Close]
BLAST of CsaV3_3G036550 vs. Swiss-Prot
Match: sp|Q40381|RNS7_NICAL (Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.1e-23
Identity = 63/188 (33.51%), Postives = 92/188 (48.94%), Query Frame = 0 

Query: 23  FEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLCQSITKYTKVL- 82
           FE+ Q V QW    C+T    +PC   P   FTIHGLWP N S     C +   +  +  
Sbjct: 24  FEYMQLVLQWPTAFCHT----TPCKRIPN-NFTIHGLWPDNVSTTLNYCAAKENFKNIED 83

Query: 83  -LLERQLNISWPDLKGG-----MNNEFWTHEWNFHGKCSDPPFSLFQYFQMSLNLLQKFD 142
              +  L   WPDL         +  FW HE+N HGKC    ++  QYF +++ L  KFD
Sbjct: 84  DTKKDDLYKRWPDLTTAETYCKQHQNFWRHEYNKHGKCCSESYNREQYFDLAMALKDKFD 143

Query: 143 VLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKTGKSQLNEIVLCLEKD 202
           +L+ L+  G+ P       +Q I + I++ T+ YP + C K    G  +L EI +C +  
Sbjct: 144 LLSSLRNHGIIPGRGMKYTVQKINSTIKKITQGYPNLSCTK----GIMELVEIGICFDSM 202

Query: 203 GATLIDCP 204
              +I+CP
Sbjct: 204 VKNVINCP 202

[Close]
BLAST of CsaV3_3G036550 vs. TrEMBL
Match: tr|A0A0A0LAN6|A0A0A0LAN6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.2e-111
Identity = 193/216 (89.35%), Postives = 203/216 (93.98%), Query Frame = 0 

Query: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60
           MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNHSNAHLLCQSITKYTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPFSL 120
           PSNHSNAHLLCQS T Y+KV+ LE QLNISWPDLK G N++FW HEW+ HGKCSDPPFSL
Sbjct: 61  PSNHSNAHLLCQSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSL 120

Query: 121 FQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKT 180
           FQYFQ+SLNLL+KFD+L ILKAAGLNPQTSQN+ IQNIM PIQRTTKKYPGIRC KNVKT
Sbjct: 121 FQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKT 180

Query: 181 GKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSIVW 217
           GKSQLNEIVLCLEKDGATLIDCPTFVS+TCAKS VW
Sbjct: 181 GKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]
BLAST of CsaV3_3G036550 vs. TrEMBL
Match: tr|A0A0A0LAB0|A0A0A0LAB0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.5e-53
Identity = 111/223 (49.78%), Postives = 140/223 (62.78%), Query Frame = 0 

Query: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60
           M Q I I+  LS ++FVN G+PF+FFQ V+QWGPN+C+     S C+  PQ  FTIHGLW
Sbjct: 1   MAQHIFILCLLSSLLFVN-GIPFDFFQLVQQWGPNICSPAG--SKCYAQPQPMFTIHGLW 60

Query: 61  PSNHSNAHLLCQSITK---YTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPP 120
           PSN S   +LC    K     KV  LE+ LN  WPD+  G N+ FW HEW  H  C DPP
Sbjct: 61  PSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPP 120

Query: 121 FSLFQYFQMSL--NLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCY 180
           F++F YF +SL   + + +D+L IL  AGL+ QT +++  QN++ PI+  T   PGIRC 
Sbjct: 121 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 180

Query: 181 KNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAK--SIVW 217
            N  T K QL EIVLC   DG TLIDCP+FVS+TC      VW
Sbjct: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVW 220

[Close]
BLAST of CsaV3_3G036550 vs. TrEMBL
Match: tr|A0A0A0LDS5|A0A0A0LDS5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G743990 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.9e-36
Identity = 79/186 (42.47%), Postives = 120/186 (64.52%), Query Frame = 0 

Query: 23  FEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLCQSITKY--TKV 82
           ++FFQ V+QWGP  C++G RV  CH  P+  FTIHGLWPSN ++  L   S+  +  +++
Sbjct: 21  YQFFQMVQQWGPAKCSSG-RVK-CHVTPKPMFTIHGLWPSNFTDLMLHYCSLQSFDASQI 80

Query: 83  LLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPFSLFQYFQMSLNL--LQKFDVLA 142
             L+ +L+  WPD+  G + +FW HEW  HG CS+PPF++FQYF+++LN+   +K+D++A
Sbjct: 81  KTLQSELSKYWPDVVKGKDVDFWKHEWEKHGTCSNPPFNIFQYFELALNIRKYKKYDLMA 140

Query: 143 ILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCY-KNVKTGKSQLNEIVLCLEKDGA 202
           IL  AGL+P TS+     +I   IQ   +  P ++C  KN +   +QL E++LC +  G 
Sbjct: 141 ILNNAGLHPSTSKLHQYDDIADLIQAAVEAKPLLKCNDKNGQGQNNQLWEVILCFDHGGV 200

Query: 203 TLIDCP 204
             IDCP
Sbjct: 201 NPIDCP 204

[Close]
BLAST of CsaV3_3G036550 vs. TrEMBL
Match: tr|A0A1S2YET8|A0A1S2YET8_CICAR (ribonuclease MC-like isoform X1 OS=Cicer arietinum OX=3827 GN=LOC101508683 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.3e-33
Identity = 78/219 (35.62%), Postives = 121/219 (55.25%), Query Frame = 0 

Query: 5   ILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNH 64
           ++II F+  +  +     ++FF+  EQW P  C    R   C   P  KFTIHGLWPSN 
Sbjct: 16  LVIILFICFIFPLPCLASYQFFKMAEQWPPTFC----RNKHCSVIPPGKFTIHGLWPSNQ 75

Query: 65  SNAH-----LLCQSITKYTKVLL--LERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPP 124
           ++            + K+TK ++  LE QL++SWP+LKG  N +FW +EWN HG CS   
Sbjct: 76  TSLRSPKCVTKDPQLQKFTKAMISKLETQLDLSWPNLKGQSNEQFWAYEWNAHGICSSNM 135

Query: 125 FSLFQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKN 184
           F+  QYF+++LN+  ++D+  ILK  G++P     I +   +T I+   +K+  +  +  
Sbjct: 136 FNQTQYFELALNIWSRYDLFDILKQEGISP----GIKVSYDITKIRNAIRKHINLNVFPQ 195

Query: 185 VKTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSIVW 217
           +    S+L EI LCL++ G T I CPT     C  +++W
Sbjct: 196 IHCVNSELLEIRLCLDQSGNTYIRCPT--PGNCGNTVLW 224

[Close]
BLAST of CsaV3_3G036550 vs. TrEMBL
Match: tr|Q9SXG2|Q9SXG2_PRUAV (S4-RNase OS=Prunus avium OX=42229 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.4e-32
Identity = 78/206 (37.86%), Postives = 115/206 (55.83%), Query Frame = 0 

Query: 2   TQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTP-PQAKFTIHGLW 61
           T + L++ F   + +V     +++FQFV+QW P  C    R  PC  P P   FTIHGLW
Sbjct: 7   TLAFLVLAFAFFICYVMSSGSYDYFQFVQQWPPTNCR--VRNKPCTKPRPLQNFTIHGLW 66

Query: 62  PSNHSNAHLLCQ---SITKYTKVL-LLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDP 121
           PSN+SN  +  +   S+  + KV   L   L ISWPD++ G +  FW  EWN HG+CS+ 
Sbjct: 67  PSNYSNPRMPSKCTGSLFNFRKVYPQLRSDLKISWPDVESGNDTRFWESEWNKHGRCSEA 126

Query: 122 PFSLFQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYK 181
             +  QYF+ S  +   +++  ILK A + P  ++N    +I++PI+R TK+ P +RC  
Sbjct: 127 SLNQMQYFERSHAMWISYNITEILKNASIVPSATKNWTYSDIVSPIKRATKRTPLLRCKY 186

Query: 182 NVKTGKSQLNEIVLCLEKDGATLIDC 203
           +  T    L+E+V C E D    IDC
Sbjct: 187 DKST--QLLHEVVFCYEYDALKQIDC 208

The following BLAST results are available for this feature:
BLAST of CsaV3_3G036550 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN59020.13.3e-11189.35hypothetical protein Csa_3G745530 [Cucumis sativus][more]
XP_011651927.15.3e-5349.78PREDICTED: ribonuclease MC-like [Cucumis sativus] >KGN58920.1 hypothetical prote... [more]
XP_022934593.11.6e-4446.08ribonuclease MC-like [Cucurbita moschata][more]
XP_022932791.12.0e-4444.55ribonuclease MC-like [Cucurbita moschata][more]
XP_023514499.12.7e-4448.47ribonuclease MC-like [Cucurbita pepo subsp. pepo][more]
BLAST of CsaV3_3G036550 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G02990.11.2e-2732.09ribonuclease 1[more]
AT1G14220.17.5e-2533.84Ribonuclease T2 family protein[more]
AT1G26820.15.9e-2233.16ribonuclease 3[more]
AT2G39780.11.1e-1530.73ribonuclease 2[more]
BLAST of CsaV3_3G036550 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|P23540|RNMC_MOMCH2.8e-2933.84Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1[more]
sp|P42813|RNS1_ARATH2.2e-2632.09Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
sp|P80022|RNLE_SOLLC3.2e-2530.41Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
sp|Q38717|RNS4_ANTHI6.1e-2432.62Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1[more]
sp|Q40381|RNS7_NICAL5.1e-2333.51Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1[more]
BLAST of CsaV3_3G036550 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LAN6|A0A0A0LAN6_CUCSA2.2e-11189.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1[more]
tr|A0A0A0LAB0|A0A0A0LAB0_CUCSA3.5e-5349.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1[more]
tr|A0A0A0LDS5|A0A0A0LDS5_CUCSA3.9e-3642.47Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G743990 PE=3 SV=1[more]
tr|A0A1S2YET8|A0A1S2YET8_CICAR5.3e-3335.62ribonuclease MC-like isoform X1 OS=Cicer arietinum OX=3827 GN=LOC101508683 PE=3 ... [more]
tr|Q9SXG2|Q9SXG2_PRUAV3.4e-3237.86S4-RNase OS=Prunus avium OX=42229 PE=2 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0033897ribonuclease T2 activity
GO:0003723RNA binding
Vocabulary: INTERPRO
TermDefinition
IPR033697Ribonuclease_T2_eukaryotic
IPR018188RNase_T2_His_AS_1
IPR036430RNase_T2-like_sf
IPR001568RNase_T2-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0005575 cellular_component
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_3G036550.1CsaV3_3G036550.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 25..202
e-value: 2.0E-39
score: 135.6
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 5..213
IPR036430Ribonuclease T2-like superfamilyGENE3DG3DSA:3.90.730.10coord: 23..215
e-value: 6.8E-50
score: 171.6
IPR036430Ribonuclease T2-like superfamilySUPERFAMILYSSF55895Ribonuclease Rh-likecoord: 20..216
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 54..61
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 23..216
e-value: 4.29793E-55
score: 175.598

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CsaV3_3G036550Cucsa.378920Cucumber (Gy14) v1cgycucB573
CsaV3_3G036550Cucsa.378780Cucumber (Gy14) v1cgycucB574
CsaV3_3G036550CsGy3G031730Cucumber (Gy14) v2cgybcucB117
CsaV3_3G036550Lsi03G019710Bottle gourd (USVL1VR-Ls)cuclsiB202
CsaV3_3G036550Cla97C10G202850Watermelon (97103) v2cucwmbB185
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsaV3_3G036550Cucumber (Chinese Long) v3cuccucB136
CsaV3_3G036550Silver-seed gourdcarcucB0181
CsaV3_3G036550Silver-seed gourdcarcucB0279
CsaV3_3G036550Cucumber (Gy14) v2cgybcucB268
CsaV3_3G036550Cucumber (Gy14) v1cgycucB170
CsaV3_3G036550Cucurbita maxima (Rimu)cmacucB0279
CsaV3_3G036550Cucurbita maxima (Rimu)cmacucB0966
CsaV3_3G036550Cucurbita maxima (Rimu)cmacucB1014
CsaV3_3G036550Cucurbita moschata (Rifu)cmocucB0270
CsaV3_3G036550Cucurbita pepo (Zucchini)cpecucB1057
CsaV3_3G036550Cucurbita moschata (Rifu)cmocucB0954
CsaV3_3G036550Cucurbita moschata (Rifu)cmocucB0997
CsaV3_3G036550Cucurbita pepo (Zucchini)cpecucB0594
CsaV3_3G036550Cucurbita pepo (Zucchini)cpecucB0747
CsaV3_3G036550Wild cucumber (PI 183967)cpicucB144
CsaV3_3G036550Wild cucumber (PI 183967)cpicucB334
CsaV3_3G036550Bottle gourd (USVL1VR-Ls)cuclsiB185
CsaV3_3G036550Bottle gourd (USVL1VR-Ls)cuclsiB203
CsaV3_3G036550Melon (DHL92) v3.5.1cucmeB213
CsaV3_3G036550Melon (DHL92) v3.5.1cucmeB218
CsaV3_3G036550Melon (DHL92) v3.5.1cucmeB261
CsaV3_3G036550Melon (DHL92) v3.6.1cucmedB211
CsaV3_3G036550Melon (DHL92) v3.6.1cucmedB256
CsaV3_3G036550Watermelon (Charleston Gray)cucwcgB197
CsaV3_3G036550Watermelon (Charleston Gray)cucwcgB199
CsaV3_3G036550Watermelon (Charleston Gray)cucwcgB224
CsaV3_3G036550Watermelon (97103) v1cucwmB207
CsaV3_3G036550Watermelon (97103) v1cucwmB208
CsaV3_3G036550Watermelon (97103) v1cucwmB275
CsaV3_3G036550Watermelon (97103) v2cucwmbB187
CsaV3_3G036550Watermelon (97103) v2cucwmbB205
CsaV3_3G036550Wax gourdcucwgoB240
CsaV3_3G036550Wax gourdcucwgoB336