CsaV3_3G013920 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_3G013920 vs. NCBI nr
Match: XP_004134170.2 (PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Cucumis sativus] >KGN57044.1 hypothetical protein Csa_3G150240 [Cucumis sativus])

HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 901/901 (100.00%), Postives = 901/901 (100.00%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120
           AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA
Sbjct: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120

Query: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180
           DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240
           DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300
           SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS
Sbjct: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300

Query: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360
           QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES
Sbjct: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480
           VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG
Sbjct: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540

Query: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600
           QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780

Query: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840
           XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG
Sbjct: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of CsaV3_3G013920 vs. NCBI nr
Match: XP_008438753.1 (PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Cucumis melo])

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 857/901 (95.12%), Postives = 862/901 (95.67%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVAKPSCPSTSNHG SSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGSSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120
           AEKFEISSSMGQS GCDLC ISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDE QDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180
           DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240
           DNTLAFLR GQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300
           +V+LACAMDSDPCNALVCDGTSNES  NT+PQLKAEVKSSEMNLPKPVKPISPIS AHGS
Sbjct: 241 NVSLACAMDSDPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360
           Q DGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYG KGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480
           VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVS FSKTNL     
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540
            XXXXXXXXXXXXXXXXX                     NCNPESSTSSFVEKP EKF+G
Sbjct: 481 AXXXXXXXXXXXXXXXXXTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600
           QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780

Query: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840
           XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRN+TDHKESWSPNVSESMKDLYKYSGTGG
Sbjct: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of CsaV3_3G013920 vs. NCBI nr
Match: XP_022952723.1 (putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita moschata] >XP_022952799.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita moschata] >XP_022952880.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita moschata] >XP_022952965.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 775/901 (86.02%), Postives = 827/901 (91.79%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVAKPSCP+TSNHGPSS+ VQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSY+FS
Sbjct: 1   MASMVAKPSCPATSNHGPSSVLVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYDFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120
           AEKFEISSS+GQ   CDLCSISQEFSAGLKLDLGLSNGGS DVGI+WPRGELEVDE QD 
Sbjct: 61  AEKFEISSSLGQIGACDLCSISQEFSAGLKLDLGLSNGGSPDVGIDWPRGELEVDEFQDV 120

Query: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180
           DWSDLTEAQL EL+L NLDTIFK AIKK+VASG+TEEVAIKA+SRSGICFG KD +SNVV
Sbjct: 121 DWSDLTEAQLVELILCNLDTIFKAAIKKVVASGFTEEVAIKAISRSGICFGCKDIMSNVV 180

Query: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240
           DNTLAFLR GQEID SREHYFEDLQQLEKYILAELVCVLRE+RP+FSTGDAMWCLL+SDM
Sbjct: 181 DNTLAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPYFSTGDAMWCLLVSDM 240

Query: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300
           SV+ AC MDSDP NA VCDGTSNE S+N+ PQLKAE KSSE+N PKP+KPISPISCAH S
Sbjct: 241 SVSHACTMDSDPSNAGVCDGTSNEGSTNSTPQLKAEAKSSELNFPKPLKPISPISCAHSS 300

Query: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360
           Q DGPAT+GVP+++KPKDPLFSS  +S+KELQNS  DV  ESF+VA N QTSV+EEKI S
Sbjct: 301 QSDGPATLGVPNLTKPKDPLFSSVSVSDKELQNSASDVARESFNVAGNPQTSVAEEKIGS 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SR+VHSNITKREYMLRQKSLHVDKNFRTYG KG SRAGK+TGLG LMLDKKLKSVSGSTA
Sbjct: 361 SRRVHSNITKREYMLRQKSLHVDKNFRTYGPKGPSRAGKVTGLGSLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480
           VNFKNASLKISKAMGIDVAQDNG+HN +T+DIPSSSL FNLEN  T SPFSK N+     
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGNHNPTTIDIPSSSLSFNLENNKTASPFSKINVLSSMP 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540
               XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX CN E STSSFVEKP EK +G
Sbjct: 481 APSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCNTEPSTSSFVEKPYEKSLG 540

Query: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600
           QWFP+DKKDEMVL L+PR +ELQ+QLQEW +WANQKVMQAARRLSKDKAE XXXXXXXXX
Sbjct: 541 QWFPKDKKDEMVLKLVPRARELQSQLQEWMEWANQKVMQAARRLSKDKAELXXXXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX+MEVAKLR
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720
           ATESAA++QEVSKREKKTL+ VQSWEK KMLFQEEHT EKRK+K+LIQELEQARD+QEQL
Sbjct: 661 ATESAATFQEVSKREKKTLVNVQSWEKTKMLFQEEHTVEKRKLKQLIQELEQARDVQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780
           EGR K+E RAKDELL+QAASLRKEREQIE S+K K  XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 721 EGRGKMEARAKDELLMQAASLRKEREQIEASLKAKEDXXXXXXXXXXXXXXXXXXXXXXX 780

Query: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840
           XXXXXXXXXX RIAALKRGIDGSYASRL DTRN+TDH ESW+PNVSESMKDLY+YSGTG 
Sbjct: 781 XXXXXXXXXXXRIAALKRGIDGSYASRLIDTRNSTDHNESWTPNVSESMKDLYEYSGTGS 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRY R
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYTR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of CsaV3_3G013920 vs. NCBI nr
Match: XP_023539181.1 (putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023539182.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023539183.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023539184.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 773/901 (85.79%), Postives = 824/901 (91.45%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVAKPSCP+ SNHGPSS+ VQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSY+FS
Sbjct: 1   MASMVAKPSCPANSNHGPSSVLVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYDFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120
           AEKFEISSS+GQ   CDLCS SQEFSAGLKLDLGLSNGGS DVGI+WPRGELEVDE QD 
Sbjct: 61  AEKFEISSSLGQIGACDLCSTSQEFSAGLKLDLGLSNGGSPDVGIDWPRGELEVDEFQDV 120

Query: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180
           DWSDLTEAQL EL+L NLDTIFK AIKK+VASG+TEEVAIKAVSRSGICFG KD +SNVV
Sbjct: 121 DWSDLTEAQLVELILCNLDTIFKAAIKKVVASGFTEEVAIKAVSRSGICFGCKDIMSNVV 180

Query: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240
           DNTLAFLR GQEID SREHYFEDLQQLEKYILAELVCVLRE+RP+FSTGDAMWCLL+SDM
Sbjct: 181 DNTLAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPYFSTGDAMWCLLVSDM 240

Query: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300
           SV+ AC MDSDP NA+VCDGTSNE S+N+ PQLKAE KSSE+N PKP+KPISPISCAH S
Sbjct: 241 SVSHACTMDSDPSNAVVCDGTSNEGSTNSTPQLKAEAKSSELNFPKPLKPISPISCAHSS 300

Query: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360
           Q DGPAT+GVP+++KPKDPLF+S  +S+KELQNST DV  ESF+V  N QTSVSEEK  S
Sbjct: 301 QSDGPATLGVPNLTKPKDPLFTSVSVSDKELQNSTSDVARESFNVGGNPQTSVSEEKTGS 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SR+VHSNITKREYMLRQKSLHVDKNFRTYG KG SRAGK+TGLG LMLDKKLKSVSGSTA
Sbjct: 361 SRRVHSNITKREYMLRQKSLHVDKNFRTYGPKGPSRAGKVTGLGSLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480
           VNFKNASLKISKAMGIDVAQDNG+HN +T+DIPSSSL FNLEN    SPFSK N+     
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGNHNPTTIDIPSSSLSFNLENNKNASPFSKINVLSSMP 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540
               XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX CN E STSSFVEKP EK +G
Sbjct: 481 APSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCNAEPSTSSFVEKPYEKSLG 540

Query: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600
           QWFP+DKKDEMVL L+PR +ELQ+QLQEW +WANQKVMQAARRLSKDKAE XXXXXXXXX
Sbjct: 541 QWFPKDKKDEMVLKLVPRARELQSQLQEWMEWANQKVMQAARRLSKDKAELXXXXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX+MEVAKLR
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720
           ATESAA+YQEVSKREKKTL+ VQSWEK KMLFQEEHT EKRK+K+LIQELEQARD+QEQL
Sbjct: 661 ATESAATYQEVSKREKKTLVNVQSWEKTKMLFQEEHTVEKRKLKQLIQELEQARDVQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780
           EGR K+E RAKDELL+QAASLRKEREQIE S+K K  XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 721 EGRGKMEARAKDELLMQAASLRKEREQIEASLKAKEDXXXXXXXXXXXXXXXXXXXXXXX 780

Query: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840
           XXXXXXXXXX RIAALKRGIDGSYASRL DTRN+TDH ESW+PNVSESMKDLY+YSGTG 
Sbjct: 781 XXXXXXXXXXXRIAALKRGIDGSYASRLVDTRNSTDHNESWTPNVSESMKDLYEYSGTGS 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRY R
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYTR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of CsaV3_3G013920 vs. NCBI nr
Match: XP_022973631.1 (putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita maxima] >XP_022973633.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita maxima] >XP_022973634.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita maxima] >XP_022973635.1 putative E3 ubiquitin-protein ligase RF298 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 770/901 (85.46%), Postives = 824/901 (91.45%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVAKPSCP+TSNHGPSS+ VQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSY+FS
Sbjct: 1   MASMVAKPSCPATSNHGPSSVLVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYDFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120
           AEKFEISSS+GQ   CDLCSISQEFSAGLKLDLGLSNGGS D+GI+WPRGELEVDE QD 
Sbjct: 61  AEKFEISSSLGQIGACDLCSISQEFSAGLKLDLGLSNGGSPDIGIDWPRGELEVDEFQDV 120

Query: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180
           DWSDLTEAQL EL+L NLDTIFK AIKK+VASG+TEEVAIKAVSRSGICFG KD +SNVV
Sbjct: 121 DWSDLTEAQLVELILCNLDTIFKAAIKKVVASGFTEEVAIKAVSRSGICFGCKDIMSNVV 180

Query: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240
           DNTLAFLR GQEID SREHYFEDLQQLEKYILAELVCVLRE+RP+FSTGDAMWCLL+SDM
Sbjct: 181 DNTLAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPYFSTGDAMWCLLVSDM 240

Query: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300
           SV+ AC MDSDP NA+VCDG+SNE S+N+ PQL+AE KSSE+N PKP+KPISPISCAH S
Sbjct: 241 SVSHACTMDSDPSNAVVCDGSSNEGSTNSTPQLEAEAKSSELNFPKPLKPISPISCAHSS 300

Query: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360
           Q DGPAT+G P+++KPKDPLF+S  +S+KELQNST DV  ESF+V  N QTSVSEEKI S
Sbjct: 301 QSDGPATLGAPNLTKPKDPLFTSVSVSDKELQNSTSDVARESFNVGGNPQTSVSEEKIGS 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SR+VHSNI KREYMLRQKSLHVDKNFRTYG KG SRAGK+TGLG LMLDKKLKSVSGSTA
Sbjct: 361 SRRVHSNIMKREYMLRQKSLHVDKNFRTYGQKGPSRAGKVTGLGSLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480
           VNFKNASLKISKAMGIDVAQDNG+HN +T+DIPSSSL FNLEN  TVSPFSK N+     
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGNHNPTTIDIPSSSLSFNLENNKTVSPFSKINVLSSMP 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540
                 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX CN E STSSFVEKP EK +G
Sbjct: 481 APSSPLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCNAEPSTSSFVEKPYEKSLG 540

Query: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600
           QWFP+DKKDEMVL L+PR +ELQ+QLQEW +WANQKVMQAARRLSKDKAE XXXXXXXXX
Sbjct: 541 QWFPKDKKDEMVLKLVPRARELQSQLQEWMEWANQKVMQAARRLSKDKAELXXXXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX+MEVAKLR
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720
           ATESAA+YQEVSKREKKTL+ VQSWEK KMLFQEEHT EKRK+K+LIQELEQARD+QEQL
Sbjct: 661 ATESAATYQEVSKREKKTLVNVQSWEKTKMLFQEEHTVEKRKLKQLIQELEQARDVQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780
           EGR K+E RAKDELL+QAASLRKEREQIE S+K K  XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 721 EGRGKMEARAKDELLMQAASLRKEREQIEASLKAKEDXXXXXXXXXXXXXXXXXXXXXXX 780

Query: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840
           XXXXXXXXXX RIAALKRGIDGSYASRL DTRN+TDH ESW+PNVSESMKDLY+YSGTG 
Sbjct: 781 XXXXXXXXXXXRIAALKRGIDGSYASRLIDTRNSTDHNESWTPNVSESMKDLYEYSGTGS 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRY R
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYTR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of CsaV3_3G013920 vs. TAIR10
Match: AT4G03000.1 (RING/U-box superfamily protein)

HSP 1 Score: 334.3 bits (856), Expect = 2.3e-91
Identity = 344/887 (38.78%), Postives = 480/887 (54.11%), Query Frame = 0

Query: 24  QEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFSAEKFEISSSMGQSSGCDLCSISQ 83
           Q+KG +NKRK  ADP          S Q+     EF   ++E+ S   QS  C+      
Sbjct: 29  QDKGRKNKRKL-ADP----------SPQNAASLTEF--PRYELHSFKSQSPLCE------ 88

Query: 84  EFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDADWSDLTEAQLEELVLINLDTIFK 143
                                 N   G+L+ +E     W D     LE L+  NL T+F+
Sbjct: 89  ----------------------NDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFR 148

Query: 144 GAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVVDNTLAFLRRGQEIDHSREHYFED 203
            A+ +I+  GY+E+V +KA+S S    GG D VSN+V++TL+FL+ G+++  SR++ FED
Sbjct: 149 SAMNQIMDCGYSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFED 208

Query: 204 LQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDMSVALACAMDSDPCNALVCDGTSN 263
           LQQL  Y L E + ++RE+RP  ST +AMW LLI D++V  A  +D+D    L     SN
Sbjct: 209 LQQLVAYSLVEKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDAD---GLEGSSVSN 268

Query: 264 ESSSNTIPQLKAE-VKSSEMNLPKPVKPISPISCAHGSQYDGPATVGVPSISKPKDPLFS 323
            S S+  P  +    KSS+ + PK     +P+S     Q +        +++  K+P  +
Sbjct: 269 ASKSSESPVAECNPPKSSDADNPK-----APVSNTQSKQSEPVKFGNFANVNNSKNP-HA 328

Query: 324 SGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIESSRKVHSNITKREY-MLRQKSLH 383
           SG    KE+ + +    E + S    S TSVS+EK+ S RK     TK+E  MLRQKS  
Sbjct: 329 SGATPGKEVFSVSTASGEGTKSA---SLTSVSDEKLVSCRK---GRTKKEMAMLRQKSC- 388

Query: 384 VDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKI-SKAMGIDVAQ 443
           V+K  RTY   G  +  K    GG +++K+ KS S   +   +N+S KI ++ M I +A+
Sbjct: 389 VEK-IRTYSKGGGYKTAK---FGGFLVEKRGKSASDLLSAQARNSSSKITTEVMKIPLAE 448

Query: 444 DNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXXXXXXXXXXXXXXXXXXXXXX 503
            + + + ST    S S   +++   T  P +                             
Sbjct: 449 SSSTLSNST---KSDSPALDVKEHVTALPANNA--------------------------- 508

Query: 504 XXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIGQWFPRDKKDEMVLNLLPRVQ 563
                              + + + +   +   P +  +G + PR+K+DE++L L+PR++
Sbjct: 509 -PAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVPRMK 568

Query: 564 ELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 623
           +LQ +LQ+WT WANQKV QA  RL KD+ E   XXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 569 DLQKELQDWTDWANQKVKQATVRLLKDQPELKAXXXXXXXXXXXXXXXXXXXXXXXXXXX 628

Query: 624 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLRATESAASYQEVSKREKKTLM 683
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX+ E A +RA+ESA S +E  +R ++ L 
Sbjct: 629 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEREAANIRASESAESCREAKERVQRLLK 688

Query: 684 KVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQLEGRWKLEERAKDELLVQAAS 743
             QSWE QK L QEE  +++ KV  L QE+ +A+  Q Q+E  WK E+ A  +L  QAA+
Sbjct: 689 NSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIEATWKQEKSATGKLTAQAAA 748

Query: 744 LRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRIAALKRGI 803
           L+KER ++E+  K +                                    +IAALK+GI
Sbjct: 749 LKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDSLKIAALKKGI 808

Query: 804 DGS------YASRLTDTR-NNTDHKESWSPNVSESMKDLYKYSGTGGVKRERECVMCLSE 863
           DG+        +  T+T+ N+    + W  N     K          +KRERECVMCLSE
Sbjct: 809 DGNNDXXXXXMNHTTNTKANSMASAKVWENNQGAESK----------IKRERECVMCLSE 813

Query: 864 EMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 901
           EMSV+FLPCAHQV+C+ CN+LHEK+ M+DCPSCR+ IQRRI  R+AR
Sbjct: 869 EMSVIFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813

BLAST of CsaV3_3G013920 vs. TAIR10
Match: AT1G03365.1 (RING/U-box superfamily protein)

HSP 1 Score: 313.9 bits (803), Expect = 3.2e-85
Identity = 301/896 (33.59%), Postives = 427/896 (47.66%), Query Frame = 0

Query: 20  SMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFSAEKFEISSSMGQSSGCDLC 79
           +++ QEKG +NKRK      L D ++  +SS  + P YE  +                  
Sbjct: 24  TVSPQEKGRKNKRK------LADPSQPNASSLTEFPPYELPS------------------ 83

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDADWSDLTEAQLEELVLINLD 139
                    LK    LS  GS    +     +L+V+  +  +W D     LEEL+  NL 
Sbjct: 84  ---------LKPQNHLSGNGS----VGEVSNQLQVEVSESVEWDDPFACHLEELLSSNLL 143

Query: 140 TIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVVDNTLAFLRRGQEIDHSREH 199
           T+F   +K+++  GYT++  +KAVSR  +  GG + +SN+V+NTL+ L+ G E   S ++
Sbjct: 144 TLFLDTMKQLIDLGYTDDEVLKAVSRCRLYCGGNNLLSNIVNNTLSALKTGDEGAGSGDY 203

Query: 200 YFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDMSVALACAMDSDPCNALVCD 259
            FEDLQQL  Y L E++ +++E+RP  ST +AMW LL+ D++V  A   + D    LV  
Sbjct: 204 VFEDLQQLVSYTLVEMISLIKEVRPSLSTVEAMWRLLMCDLNVLQAFEAEGD---GLVSS 263

Query: 260 GTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGSQYDGPATVGVPSISKPKDP 319
              ++S S            +E N PK   P            D P        S   +P
Sbjct: 264 SKLSDSES----------LGAESNPPKSSDP------------DNPXXXXXDPQSNRNEP 323

Query: 320 L----FSSGPLSEKELQNSTFDVVEESFSVAVNSQ-------TSVSEEKIESSRKVHSNI 379
           L    F + P S+K   + T    E      V+ Q       T VS+EK+ S RK     
Sbjct: 324 LKFGNFPNTPNSKKTQSSGTTPGKEVCSGSTVSCQGMRSTSFTLVSDEKLVSCRK---GR 383

Query: 380 TKREY-MLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNAS 439
           TK+E  MLRQKS  V+K  RTY      +A K   +G  +L+K++KS S       +N+S
Sbjct: 384 TKKEIAMLRQKSC-VEK-IRTYSKGSGYKAAKFASVGSFLLEKRVKSSSEFVP---RNSS 443

Query: 440 LKISKAMGIDV--AQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXXXXXXX 499
            KI+  +G+ V  A+D+G        + S  +  + +   T  P    ++          
Sbjct: 444 SKITAEIGVKVSLAEDSGCFVRKNSKLDSPVVVVDAKGYITALP--ARSVKSASKKKTGS 503

Query: 500 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIGQWFPR 559
                       XXXXXXXXXXXXXXXXXX    + + + +   +   P +  +G + PR
Sbjct: 504 ESVTLIPSASEKXXXXXXXXXXXXXXXXXXEEKASVSAKLAPDYYAGIPYDAALGIYVPR 563

Query: 560 DKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXXXXXXX 619
           DKKDE++L L+PRV +LQN+LQ WT WANQKV +A  RL KD+ E          XXXXX
Sbjct: 564 DKKDELILKLVPRVNDLQNELQVWTDWANQKVKEATGRLLKDQPELKALRKEREEXXXXX 623

Query: 620 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLRATESA 679
           XX                                             +ME AK++A ESA
Sbjct: 624 XXKQLLEENTRKRLSEMDFALKNATSQLEKAFNTAHRLELEQSILKKEMEAAKIKAVESA 683

Query: 680 ASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQLEGRWK 739
            S++E  +R +++L  + SWE QK++ QEE   ++ KV  L +E+ +A++ Q Q+E   K
Sbjct: 684 ESFREAKERGERSLKDIHSWEGQKIMLQEELKGQREKVTVLQKEVTKAKNRQNQIEAALK 743

Query: 740 LEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 799
            E  AK +L  QA+ +RKE +++E   KV+                              
Sbjct: 744 QERTAKGKLSAQASLIRKETKELEALGKVEEERIKGKAETDVKYYIDNIKRLEREISELK 803

Query: 800 XXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGGVKRER 859
                SRI ALK+G   S A++                   ES+       G   VKRER
Sbjct: 804 LKSDYSRIIALKKGSSESKATK------------------RESL-------GMPKVKRER 822

Query: 860 ECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYARS 902
           ECVMCLSEEMSV+FLPCAHQV+C  CN+LHEK+GM DCPSCR  I RRI  R+ARS
Sbjct: 864 ECVMCLSEEMSVIFLPCAHQVLCFKCNQLHEKEGMMDCPSCRGTIHRRIQARFARS 822

BLAST of CsaV3_3G013920 vs. TAIR10
Match: AT1G32530.1 (RING/U-box superfamily protein)

HSP 1 Score: 111.3 bits (277), Expect = 3.1e-24
Identity = 180/841 (21.40%), Postives = 306/841 (36.39%), Query Frame = 0

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDAD-WSDLTEAQLEELVLINL 139
           ++SQ     L    GL++ GS  V +N       V E+ + + W+  TE  LEE++L +L
Sbjct: 34  AMSQSIVENLVYHPGLTDSGS--VNLN------SVTENPEENFWAYCTEEHLEEILLKHL 93

Query: 140 DTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVVDNTLAFLR-------RGQ 199
           + ++  A+ K++  GY E VA+KAV  +G C+G  D ++N+V+N+L++L           
Sbjct: 94  EFLYNQAVSKLLELGYEERVALKAVLSNGHCYGELDVLTNIVNNSLSYLNSXXXXXXXXX 153

Query: 200 EIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDMSVALACAMDSD 259
                 E  F DL+ LE+Y LA ++ +L++++P  S GDAMWCLL+S++ V  A  +D  
Sbjct: 154 XXXXXTETGFTDLRDLEEYSLAGMIYLLQQVKPNLSKGDAMWCLLMSELHVGRASTLDV- 213

Query: 260 PCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCA-HGSQYDGPATVGV 319
           P N   C    + +  +       ++             ++P  C  HG           
Sbjct: 214 PTNRSSCCTKEDSNVEDVGTGGTLDIAGF----------MAPALCRFHGGW--------- 273

Query: 320 PSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIESSRKVHSNITK 379
                   P FS    S K  +                      + +I+  ++ + + + 
Sbjct: 274 -GFGNGGGPEFSGNGFSMKGAELKL-------------------QREIDCPKRFNLSPSM 333

Query: 380 REYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNA--SL 439
           +  + R        N   + A             G     K K +  S  +    A    
Sbjct: 334 KSLLKR--------NVAAFAA-------------GYRASMKQKQIQSSDTIGDSKACNDP 393

Query: 440 KISKAMGIDVAQDNGSHNLSTM--DIPSSSLPFNLENINTVSPFSKTNLXXXXXXXXXXX 499
            I K+ G    +     ++ST+       +L  NLE++                      
Sbjct: 394 AIVKSCGQQPRKSGSEESVSTVLEKFRDLNLDDNLESVGV-------------------- 453

Query: 500 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIGQWFPRD 559
                                                                      D
Sbjct: 454 -----------------------------------------------------------D 513

Query: 560 KKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXXXXXXXX 619
            KD ++++LL +V++ + +++E  +WA +  MQAA+++S++ AE                
Sbjct: 514 DKDCVIVDLLHQVKDFEKKVKERKEWAQKNAMQAAQKVSEELAELKTLSSEREGIQLLKK 573

Query: 620 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLRATESAA 679
                                                         + E +KL A+ES  
Sbjct: 574 GKQAVEESTAKRFTDKEIELRKACSQNDRANVIVRKLENQNAEIRAEREGSKLSASESLK 633

Query: 680 SYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQLEGRWKL 739
           +  E SK+EKK L K+ +WEKQ +  Q+E TAEK K+K L + L Q  +           
Sbjct: 634 ACMEASKKEKKCLKKLVAWEKQILKLQDEITAEKEKIKALYKTLAQITEYXXXXXXXXXX 693

Query: 740 EERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 799
                                     K K                               
Sbjct: 694 XXXXXXXXXXXXXXXXXXXXXXXGHNKRK------------LETLRLKIELDFQRHKDDH 711

Query: 800 XXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGT--GGVKRE 859
                 +  LK   D S +S +++  N    K+S   N+++ ++++ K  G+       +
Sbjct: 754 QRLEQELGRLKASSD-SDSSHISN--NAWKPKKSQGENIAKLLEEIDKLEGSYDNEANYD 711

Query: 860 RECVMCLSEEMSVVFLPCAHQVVCTTCNE---LHEKQGMK-DCPSCRSPIQRRIPVRYAR 902
           REC++C+ +E+SVVFLPCAHQVVC +C++        G K  CP CR  +Q+RI +  A 
Sbjct: 814 RECIICMKDEVSVVFLPCAHQVVCGSCSDSFFASNNGGSKVTCPCCRGLVQQRIRIFGAT 711

BLAST of CsaV3_3G013920 vs. TAIR10
Match: AT2G35330.1 (RING/U-box superfamily protein)

HSP 1 Score: 95.9 bits (237), Expect = 1.4e-19
Identity = 218/801 (27.22%), Postives = 325/801 (40.57%), Query Frame = 0

Query: 122 WSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVVD 181
           W   TE QLE+++L +L+ ++  AI K+V SGY E+VA++AV  +G C+GG D ++N++ 
Sbjct: 98  WGYCTEEQLEDILLKHLEYLYNEAISKLVGSGYDEDVALRAVLSNGYCYGGMDVMTNILH 157

Query: 182 NTLAFLR----RGQEIDH--SREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCL 241
           N+LA+L+     G  +++    E  F DL+QLE+Y LA +V +L++++P  S GDAMWCL
Sbjct: 158 NSLAYLKSNTGEGSNVNNEDQSETVFTDLRQLEEYSLAGMVYLLQQVKPNLSKGDAMWCL 217

Query: 242 LISDMSVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPIS 301
           L+S++ V  A  MD           +    SSN        V  +   +      I+P  
Sbjct: 218 LMSELHVGRASTMD--------IPSSGKGDSSN------VGVGGASSTVNGVGGAIAPAL 277

Query: 302 CA-HGSQYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVS 361
           C  HG                 K P FS    S                   ++S+    
Sbjct: 278 CRFHGGW----------GFGNGKGPKFSGNGFS-------------------LHSEELTL 337

Query: 362 EEKIESSRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKK--- 421
           + +I+  R+ + + + +  +LR+       N   + A          G    M  KK   
Sbjct: 338 QREIDCPRRFNLSPSMKS-LLRE-------NVAAFAA----------GYRASMEQKKQVQ 397

Query: 422 LKSVSGSTAVNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFS 481
           ++S +  T+++   A+    K            H   + +  SS L            F 
Sbjct: 398 MQSETSGTSLSCTAAATHSEKC--------EQPHVFGSEECFSSVL----------EKFR 457

Query: 482 KTNLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFV 541
             NL                                            + N +S+     
Sbjct: 458 DLNL--------------------------------------------DDNVDSA----- 517

Query: 542 EKPQEKFIGQWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEX 601
                       P + KD+ ++ LL +VQ+L+                           X
Sbjct: 518 ------------PEELKDDALIGLLQQVQDLKXXXXXXXXXXXXXXXXXXXXXXXXXXXX 577

Query: 602 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 661
           XXXXXXXXXXXXXXXXXXXXX                                       
Sbjct: 578 XXXXXXXXXXXXXXXXXXXXXDSTLKKLSEMENALRKASGQVDKANAVVRALENESAEIR 637

Query: 662 XDMEVAKLRATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELE 721
            +ME +KL A+ES  +  E SK+EKK L K+ +WEKQKM               L + L 
Sbjct: 638 AEMEASKLSASESLTACMEASKKEKKCLKKLLAWEKQKMXXXXXXXXXXXXXXALNRALA 697

Query: 722 QARDLQEQLEGRWK-----------LEERAKDELLVQAASLRKEREQIEDSVKVKXXXXX 781
           Q    +++ E +W+                            ++ E +    ++      
Sbjct: 698 QITQEEKEYEAKWRXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKVESLRLXXEIDFQRHK 738

Query: 782 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKES 841
           XXXXXXXXXXXXXXXXXXXXXXXXXXX   +++ + K    G   S+L +  N  D    
Sbjct: 758 XXXXXXXXXXXXXXXXXXXXXXXXXXXTSHTKVKSDKS--KGETMSKLLEELNRLDGSYE 738

Query: 842 WSPNVSESMKDLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGM 901
              N                   +REC++C+ +E+SVVFLPCAHQVVC +C++     G 
Sbjct: 818 KEANY------------------DRECLICMKDEVSVVFLPCAHQVVCASCSDSFMGSGK 738

BLAST of CsaV3_3G013920 vs. TAIR10
Match: AT1G73950.1 (Transmembrane Fragile-X-F-associated protein)

HSP 1 Score: 51.2 bits (121), Expect = 3.8e-06
Identity = 22/50 (44.00%), Postives = 30/50 (60.00%), Query Frame = 0

Query: 847 CVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPV 897
           C +C   E+SVV LPC H+V+C  C++       K CP CR  I+ R+PV
Sbjct: 419 CRVCFEREISVVLLPCRHRVLCRNCSD-----KCKKCPFCRITIEERLPV 463

BLAST of CsaV3_3G013920 vs. Swiss-Prot
Match: sp|Q0WPJ7|RF298_ARATH (Putative E3 ubiquitin-protein ligase RF298 OS=Arabidopsis thaliana OX=3702 GN=RF298 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 4.1e-90
Identity = 344/887 (38.78%), Postives = 480/887 (54.11%), Query Frame = 0

Query: 24  QEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFSAEKFEISSSMGQSSGCDLCSISQ 83
           Q+KG +NKRK  ADP          S Q+     EF   ++E+ S   QS  C+      
Sbjct: 29  QDKGRKNKRKL-ADP----------SPQNAASLTEF--PRYELHSFKSQSPLCE------ 88

Query: 84  EFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDADWSDLTEAQLEELVLINLDTIFK 143
                                 N   G+L+ +E     W D     LE L+  NL T+F+
Sbjct: 89  ----------------------NDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFR 148

Query: 144 GAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVVDNTLAFLRRGQEIDHSREHYFED 203
            A+ +I+  GY+E+V +KA+S S    GG D VSN+V++TL+FL+ G+++  SR++ FED
Sbjct: 149 SAMNQIMDCGYSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFED 208

Query: 204 LQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDMSVALACAMDSDPCNALVCDGTSN 263
           LQQL  Y L E + ++RE+RP  ST +AMW LLI D++V  A  +D+D    L     SN
Sbjct: 209 LQQLVAYSLVEKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDAD---GLEGSSVSN 268

Query: 264 ESSSNTIPQLKAE-VKSSEMNLPKPVKPISPISCAHGSQYDGPATVGVPSISKPKDPLFS 323
            S S+  P  +    KSS+ + PK     +P+S     Q +        +++  K+P  +
Sbjct: 269 ASKSSESPVAECNPPKSSDADNPK-----APVSNTQSKQSEPVKFGNFANVNNSKNP-HA 328

Query: 324 SGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIESSRKVHSNITKREY-MLRQKSLH 383
           SG    KE+ + +    E + S    S TSVS+EK+ S RK     TK+E  MLRQKS  
Sbjct: 329 SGATPGKEVFSVSTASGEGTKSA---SLTSVSDEKLVSCRK---GRTKKEMAMLRQKSC- 388

Query: 384 VDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKI-SKAMGIDVAQ 443
           V+K  RTY   G  +  K    GG +++K+ KS S   +   +N+S KI ++ M I +A+
Sbjct: 389 VEK-IRTYSKGGGYKTAK---FGGFLVEKRGKSASDLLSAQARNSSSKITTEVMKIPLAE 448

Query: 444 DNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXXXXXXXXXXXXXXXXXXXXXX 503
            + + + ST    S S   +++   T  P +                             
Sbjct: 449 SSSTLSNST---KSDSPALDVKEHVTALPANNA--------------------------- 508

Query: 504 XXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIGQWFPRDKKDEMVLNLLPRVQ 563
                              + + + +   +   P +  +G + PR+K+DE++L L+PR++
Sbjct: 509 -PAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVPRMK 568

Query: 564 ELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 623
           +LQ +LQ+WT WANQKV QA  RL KD+ E   XXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 569 DLQKELQDWTDWANQKVKQATVRLLKDQPELKAXXXXXXXXXXXXXXXXXXXXXXXXXXX 628

Query: 624 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLRATESAASYQEVSKREKKTLM 683
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX+ E A +RA+ESA S +E  +R ++ L 
Sbjct: 629 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEREAANIRASESAESCREAKERVQRLLK 688

Query: 684 KVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQLEGRWKLEERAKDELLVQAAS 743
             QSWE QK L QEE  +++ KV  L QE+ +A+  Q Q+E  WK E+ A  +L  QAA+
Sbjct: 689 NSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIEATWKQEKSATGKLTAQAAA 748

Query: 744 LRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRIAALKRGI 803
           L+KER ++E+  K +                                    +IAALK+GI
Sbjct: 749 LKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDSLKIAALKKGI 808

Query: 804 DGS------YASRLTDTR-NNTDHKESWSPNVSESMKDLYKYSGTGGVKRERECVMCLSE 863
           DG+        +  T+T+ N+    + W  N     K          +KRERECVMCLSE
Sbjct: 809 DGNNDXXXXXMNHTTNTKANSMASAKVWENNQGAESK----------IKRERECVMCLSE 813

Query: 864 EMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 901
           EMSV+FLPCAHQV+C+ CN+LHEK+ M+DCPSCR+ IQRRI  R+AR
Sbjct: 869 EMSVIFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813

BLAST of CsaV3_3G013920 vs. Swiss-Prot
Match: sp|Q9ZVT8|RF4_ARATH (Putative E3 ubiquitin-protein ligase RF4 OS=Arabidopsis thaliana OX=3702 GN=RF4 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.7e-84
Identity = 301/896 (33.59%), Postives = 427/896 (47.66%), Query Frame = 0

Query: 20  SMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFSAEKFEISSSMGQSSGCDLC 79
           +++ QEKG +NKRK      L D ++  +SS  + P YE  +                  
Sbjct: 24  TVSPQEKGRKNKRK------LADPSQPNASSLTEFPPYELPS------------------ 83

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDADWSDLTEAQLEELVLINLD 139
                    LK    LS  GS    +     +L+V+  +  +W D     LEEL+  NL 
Sbjct: 84  ---------LKPQNHLSGNGS----VGEVSNQLQVEVSESVEWDDPFACHLEELLSSNLL 143

Query: 140 TIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVVDNTLAFLRRGQEIDHSREH 199
           T+F   +K+++  GYT++  +KAVSR  +  GG + +SN+V+NTL+ L+ G E   S ++
Sbjct: 144 TLFLDTMKQLIDLGYTDDEVLKAVSRCRLYCGGNNLLSNIVNNTLSALKTGDEGAGSGDY 203

Query: 200 YFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDMSVALACAMDSDPCNALVCD 259
            FEDLQQL  Y L E++ +++E+RP  ST +AMW LL+ D++V  A   + D    LV  
Sbjct: 204 VFEDLQQLVSYTLVEMISLIKEVRPSLSTVEAMWRLLMCDLNVLQAFEAEGD---GLVSS 263

Query: 260 GTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGSQYDGPATVGVPSISKPKDP 319
              ++S S            +E N PK   P            D P        S   +P
Sbjct: 264 SKLSDSES----------LGAESNPPKSSDP------------DNPXXXXXDPQSNRNEP 323

Query: 320 L----FSSGPLSEKELQNSTFDVVEESFSVAVNSQ-------TSVSEEKIESSRKVHSNI 379
           L    F + P S+K   + T    E      V+ Q       T VS+EK+ S RK     
Sbjct: 324 LKFGNFPNTPNSKKTQSSGTTPGKEVCSGSTVSCQGMRSTSFTLVSDEKLVSCRK---GR 383

Query: 380 TKREY-MLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNAS 439
           TK+E  MLRQKS  V+K  RTY      +A K   +G  +L+K++KS S       +N+S
Sbjct: 384 TKKEIAMLRQKSC-VEK-IRTYSKGSGYKAAKFASVGSFLLEKRVKSSSEFVP---RNSS 443

Query: 440 LKISKAMGIDV--AQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXXXXXXX 499
            KI+  +G+ V  A+D+G        + S  +  + +   T  P    ++          
Sbjct: 444 SKITAEIGVKVSLAEDSGCFVRKNSKLDSPVVVVDAKGYITALP--ARSVKSASKKKTGS 503

Query: 500 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIGQWFPR 559
                       XXXXXXXXXXXXXXXXXX    + + + +   +   P +  +G + PR
Sbjct: 504 ESVTLIPSASEKXXXXXXXXXXXXXXXXXXEEKASVSAKLAPDYYAGIPYDAALGIYVPR 563

Query: 560 DKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXXXXXXX 619
           DKKDE++L L+PRV +LQN+LQ WT WANQKV +A  RL KD+ E          XXXXX
Sbjct: 564 DKKDELILKLVPRVNDLQNELQVWTDWANQKVKEATGRLLKDQPELKALRKEREEXXXXX 623

Query: 620 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLRATESA 679
           XX                                             +ME AK++A ESA
Sbjct: 624 XXKQLLEENTRKRLSEMDFALKNATSQLEKAFNTAHRLELEQSILKKEMEAAKIKAVESA 683

Query: 680 ASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQLEGRWK 739
            S++E  +R +++L  + SWE QK++ QEE   ++ KV  L +E+ +A++ Q Q+E   K
Sbjct: 684 ESFREAKERGERSLKDIHSWEGQKIMLQEELKGQREKVTVLQKEVTKAKNRQNQIEAALK 743

Query: 740 LEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 799
            E  AK +L  QA+ +RKE +++E   KV+                              
Sbjct: 744 QERTAKGKLSAQASLIRKETKELEALGKVEEERIKGKAETDVKYYIDNIKRLEREISELK 803

Query: 800 XXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGGVKRER 859
                SRI ALK+G   S A++                   ES+       G   VKRER
Sbjct: 804 LKSDYSRIIALKKGSSESKATK------------------RESL-------GMPKVKRER 822

Query: 860 ECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYARS 902
           ECVMCLSEEMSV+FLPCAHQV+C  CN+LHEK+GM DCPSCR  I RRI  R+ARS
Sbjct: 864 ECVMCLSEEMSVIFLPCAHQVLCFKCNQLHEKEGMMDCPSCRGTIHRRIQARFARS 822

BLAST of CsaV3_3G013920 vs. Swiss-Prot
Match: sp|Q8RX22|MIP1_ARATH (MND1-interacting protein 1 OS=Arabidopsis thaliana OX=3702 GN=MIP1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.6e-23
Identity = 180/841 (21.40%), Postives = 306/841 (36.39%), Query Frame = 0

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDAD-WSDLTEAQLEELVLINL 139
           ++SQ     L    GL++ GS  V +N       V E+ + + W+  TE  LEE++L +L
Sbjct: 34  AMSQSIVENLVYHPGLTDSGS--VNLN------SVTENPEENFWAYCTEEHLEEILLKHL 93

Query: 140 DTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVVDNTLAFLR-------RGQ 199
           + ++  A+ K++  GY E VA+KAV  +G C+G  D ++N+V+N+L++L           
Sbjct: 94  EFLYNQAVSKLLELGYEERVALKAVLSNGHCYGELDVLTNIVNNSLSYLNSXXXXXXXXX 153

Query: 200 EIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDMSVALACAMDSD 259
                 E  F DL+ LE+Y LA ++ +L++++P  S GDAMWCLL+S++ V  A  +D  
Sbjct: 154 XXXXXTETGFTDLRDLEEYSLAGMIYLLQQVKPNLSKGDAMWCLLMSELHVGRASTLDV- 213

Query: 260 PCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCA-HGSQYDGPATVGV 319
           P N   C    + +  +       ++             ++P  C  HG           
Sbjct: 214 PTNRSSCCTKEDSNVEDVGTGGTLDIAGF----------MAPALCRFHGGW--------- 273

Query: 320 PSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIESSRKVHSNITK 379
                   P FS    S K  +                      + +I+  ++ + + + 
Sbjct: 274 -GFGNGGGPEFSGNGFSMKGAELKL-------------------QREIDCPKRFNLSPSM 333

Query: 380 REYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNA--SL 439
           +  + R        N   + A             G     K K +  S  +    A    
Sbjct: 334 KSLLKR--------NVAAFAA-------------GYRASMKQKQIQSSDTIGDSKACNDP 393

Query: 440 KISKAMGIDVAQDNGSHNLSTM--DIPSSSLPFNLENINTVSPFSKTNLXXXXXXXXXXX 499
            I K+ G    +     ++ST+       +L  NLE++                      
Sbjct: 394 AIVKSCGQQPRKSGSEESVSTVLEKFRDLNLDDNLESVGV-------------------- 453

Query: 500 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIGQWFPRD 559
                                                                      D
Sbjct: 454 -----------------------------------------------------------D 513

Query: 560 KKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXXXXXXXX 619
            KD ++++LL +V++ + +++E  +WA +  MQAA+++S++ AE                
Sbjct: 514 DKDCVIVDLLHQVKDFEKKVKERKEWAQKNAMQAAQKVSEELAELKTLSSEREGIQLLKK 573

Query: 620 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLRATESAA 679
                                                         + E +KL A+ES  
Sbjct: 574 GKQAVEESTAKRFTDKEIELRKACSQNDRANVIVRKLENQNAEIRAEREGSKLSASESLK 633

Query: 680 SYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQLEGRWKL 739
           +  E SK+EKK L K+ +WEKQ +  Q+E TAEK K+K L + L Q  +           
Sbjct: 634 ACMEASKKEKKCLKKLVAWEKQILKLQDEITAEKEKIKALYKTLAQITEYXXXXXXXXXX 693

Query: 740 EERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 799
                                     K K                               
Sbjct: 694 XXXXXXXXXXXXXXXXXXXXXXXGHNKRK------------LETLRLKIELDFQRHKDDH 711

Query: 800 XXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGT--GGVKRE 859
                 +  LK   D S +S +++  N    K+S   N+++ ++++ K  G+       +
Sbjct: 754 QRLEQELGRLKASSD-SDSSHISN--NAWKPKKSQGENIAKLLEEIDKLEGSYDNEANYD 711

Query: 860 RECVMCLSEEMSVVFLPCAHQVVCTTCNE---LHEKQGMK-DCPSCRSPIQRRIPVRYAR 902
           REC++C+ +E+SVVFLPCAHQVVC +C++        G K  CP CR  +Q+RI +  A 
Sbjct: 814 RECIICMKDEVSVVFLPCAHQVVCGSCSDSFFASNNGGSKVTCPCCRGLVQQRIRIFGAT 711

BLAST of CsaV3_3G013920 vs. Swiss-Prot
Match: sp|Q13489|BIRC3_HUMAN (Baculoviral IAP repeat-containing protein 3 OS=Homo sapiens OX=9606 GN=BIRC3 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 1.8e-05
Identity = 24/66 (36.36%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 826 SESMKDLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPS 885
           +E + DL        ++ ER C +C+ +E+S+VF+PC H VVC  C        ++ CP 
Sbjct: 536 TEDVSDLPVEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDC-----APSLRKCPI 595

Query: 886 CRSPIQ 892
           CRS I+
Sbjct: 596 CRSTIK 596

BLAST of CsaV3_3G013920 vs. Swiss-Prot
Match: sp|O62640|PIAP_PIG (Putative inhibitor of apoptosis OS=Sus scrofa OX=9823 GN=PIAP PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.4e-05
Identity = 23/66 (34.85%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 826 SESMKDLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPS 885
           +E++ DL        ++ ER C +C+ +E+S+VF+PC H VVC  C        ++ CP 
Sbjct: 290 TENVSDLSMEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDC-----APSLRKCPI 349

Query: 886 CRSPIQ 892
           CR  I+
Sbjct: 350 CRGTIK 350

BLAST of CsaV3_3G013920 vs. TrEMBL
Match: tr|A0A0A0L7S3|A0A0A0L7S3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150240 PE=4 SV=1)

HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 901/901 (100.00%), Postives = 901/901 (100.00%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120
           AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA
Sbjct: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120

Query: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180
           DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240
           DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300
           SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS
Sbjct: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300

Query: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360
           QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES
Sbjct: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480
           VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG
Sbjct: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540

Query: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600
           QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780

Query: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840
           XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG
Sbjct: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of CsaV3_3G013920 vs. TrEMBL
Match: tr|A0A1S3AX62|A0A1S3AX62_CUCME (putative E3 ubiquitin-protein ligase RF298 OS=Cucumis melo OX=3656 GN=LOC103483769 PE=4 SV=1)

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 857/901 (95.12%), Postives = 862/901 (95.67%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVAKPSCPSTSNHG SSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGSSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120
           AEKFEISSSMGQS GCDLC ISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDE QDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180
           DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240
           DNTLAFLR GQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300
           +V+LACAMDSDPCNALVCDGTSNES  NT+PQLKAEVKSSEMNLPKPVKPISPIS AHGS
Sbjct: 241 NVSLACAMDSDPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360
           Q DGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYG KGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXXX 480
           VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVS FSKTNL     
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFIG 540
            XXXXXXXXXXXXXXXXX                     NCNPESSTSSFVEKP EKF+G
Sbjct: 481 AXXXXXXXXXXXXXXXXXTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600
           QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXXX 780

Query: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840
           XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRN+TDHKESWSPNVSESMKDLYKYSGTGG
Sbjct: 781 XXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of CsaV3_3G013920 vs. TrEMBL
Match: tr|A0A2N9IUA4|A0A2N9IUA4_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56878 PE=4 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 6.2e-261
Identity = 640/905 (70.72%), Postives = 727/905 (80.33%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVA  S  ST     SS+++Q+KGSRNKRK+RADPPLGD NKI S  Q++CPSYEFS
Sbjct: 1   MASMVATSSGSSTCQLS-SSVSIQDKGSRNKRKFRADPPLGDPNKIISLPQNECPSYEFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSN-GGSSDVGINWPRGELEVDEDQD 120
           AEKF+I+   GQ S CDLCS+SQ+ S GLKLDLGLS+  GS +VG   PR ELE DE QD
Sbjct: 61  AEKFDIAPGHGQPSACDLCSVSQDHSDGLKLDLGLSSAAGSFEVGPRRPREELEADEFQD 120

Query: 121 ADWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNV 180
           ADWSDLTEA+LEELVL NLD IFK AIKKI+A G+TEEVA KAV RSG+C+G KDTVSN+
Sbjct: 121 ADWSDLTEAELEELVLSNLDAIFKSAIKKIIACGHTEEVATKAVLRSGLCYGCKDTVSNI 180

Query: 181 VDNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISD 240
           VDN L FLR  QEID SR H FEDLQQLEKY+LAELVCVLRE+RPFFSTGDAMWCLLI D
Sbjct: 181 VDNALTFLRSSQEIDPSRGHCFEDLQQLEKYVLAELVCVLREVRPFFSTGDAMWCLLICD 240

Query: 241 MSVALACAMDSDPCNALVCDGTSNESSSN-TIPQLKAEVKSSEMNLPKPVKPISPISCAH 300
           M+V+ ACAMD DP ++ + DG S+ SS N T  Q K E KS E+NLP P K    ++C H
Sbjct: 241 MNVSHACAMDGDPLSSFMGDGASSGSSYNSTHLQSKTETKSFELNLPSPSK---QLNCPH 300

Query: 301 GSQYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKI 360
            +Q + P   GVP+I K K+ L  +G +SEK+   +T + +++SF+ A  SQ+   EEK 
Sbjct: 301 STQSEAPTVAGVPNIMKSKNSLVLNGLVSEKDGMTTTSETIDKSFNAAGTSQSPAVEEKF 360

Query: 361 ESSRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGS 420
             SRKVHS+ TKREY+LRQKSLHV+K++RTYG+KGS+R GKL+G GGL+LDKKLKSVS S
Sbjct: 361 GGSRKVHSSTTKREYILRQKSLHVEKHYRTYGSKGSTRGGKLSGFGGLILDKKLKSVSDS 420

Query: 421 TAVNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLEN--INTVSPFSKTNLX 480
            AVN K+ASLKI+K +G+DV QDNG+H+LST   PSSS   NLE+  +NT+S  SK   X
Sbjct: 421 AAVNLKSASLKINKPVGVDVPQDNGNHSLSTNSGPSSSAASNLESVTVNTISALSKXXXX 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQE 540
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX      S  SF   P +
Sbjct: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPSNCSFAGIPYD 540

Query: 541 KFIGQWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXX 600
           K +GQW PRDKKDEM+L L+PRV+ELQNQLQEWT+WANQKVMQAARRLSK+KAEXXXXXX
Sbjct: 541 KSLGQWVPRDKKDEMILKLVPRVRELQNQLQEWTEWANQKVMQAARRLSKEKAEXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEV 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX                    +ME 
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVERANSTVRRLEVENAQLRQEMEA 660

Query: 661 AKLRATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDL 720
           AKLRATESAAS  EVSKREKKTL+KVQSWEKQK LFQEE   EKRKV +L+QELEQA+DL
Sbjct: 661 AKLRATESAASCDEVSKREKKTLIKVQSWEKQKALFQEELITEKRKVTQLLQELEQAKDL 720

Query: 721 QEQLEGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXX 780
           +EQLE RWK EE+AK+ELL+QA S+RKEREQIE S K KXXXXXXXXXXXXXXXXXXXXX
Sbjct: 721 EEQLEARWKQEEKAKEELLMQANSIRKEREQIEVSAKSKXXXXXXXXXXXXXXXXXXXXX 780

Query: 781 XXXXXXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYS 840
           XXXXXXXXX     S+IAAL+RGIDGSYASRLTD RN  DH++S SP +SE ++D   Y+
Sbjct: 781 XXXXXXXXXLKTDSSKIAALRRGIDGSYASRLTDIRNGPDHQKSRSPYISEVVRDFQDYA 840

Query: 841 GTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPV 900
           G GGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRI V
Sbjct: 841 GEGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRISV 900

Query: 901 RYARS 902
           RYARS
Sbjct: 901 RYARS 901

BLAST of CsaV3_3G013920 vs. TrEMBL
Match: tr|M5XM14|M5XM14_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G146600 PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.9e-256
Identity = 649/931 (69.71%), Postives = 725/931 (77.87%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           MASMVAK +   ++   P S+TVQEKGSRNKRK+RADPPLGD NKI    Q +C SYEFS
Sbjct: 1   MASMVAKGTSSCSTQVSP-SITVQEKGSRNKRKFRADPPLGDPNKIIPLPQTECTSYEFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNG-GSSDVGINWPRGELEVDEDQD 120
           AEKFEI+   GQ   CDLC+++++ S GLKLDLGLS+  GSS+VG + PR ELE DE QD
Sbjct: 61  AEKFEITQGHGQIGVCDLCTVNKDHSDGLKLDLGLSSTVGSSEVGPSRPREELEADEFQD 120

Query: 121 ADWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNV 180
           ADWSDLTE QLEELVL NLDTIFK AIKKIVA GY EEVA KAV RSG+C+G KDTVSN+
Sbjct: 121 ADWSDLTETQLEELVLSNLDTIFKSAIKKIVACGYAEEVATKAVLRSGLCYGCKDTVSNI 180

Query: 181 VDNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISD 240
           VDNTL FLR GQEID SREH FEDLQQLEKYILAELVCVLRE+RPFFS GDAMWCLLI D
Sbjct: 181 VDNTLNFLRSGQEIDPSREHCFEDLQQLEKYILAELVCVLREVRPFFSMGDAMWCLLICD 240

Query: 241 MSVALACAMDSDPCNALVCDGTSNESSSN-TIPQLKAEVKSSEMNLPKPVKPISPISCAH 300
           M+V+ ACAMD DP N+ + DG SN SSS    PQ K E KS E+NL  P KP+  I  +H
Sbjct: 241 MNVSHACAMDGDPLNSFMSDGASNGSSSTPNQPQSKIEAKSVELNLLSPSKPVPLIPGSH 300

Query: 301 GSQYDGPATV-GVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEK 360
            SQY+ PA   GVP+I+KPK+ L  SG  SEKE+ NST    ++SF V+  SQ+S  EEK
Sbjct: 301 SSQYETPAIAGGVPNIAKPKNSLVQSGSFSEKEITNSTSHNGDKSFGVSGTSQSSAVEEK 360

Query: 361 IESSRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSG 420
           +  SRKVHS   KREYMLRQK LH++KN+RTYG KGSSRAGKL+GLGGL+LDKKLKSVS 
Sbjct: 361 LLGSRKVHSVSAKREYMLRQKPLHLEKNYRTYGCKGSSRAGKLSGLGGLILDKKLKSVSD 420

Query: 421 STAVNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVS--------- 480
           STAVN KNASLKISKAMG+DV Q+NG+HNLS+   PSS   FNL+  NT S         
Sbjct: 421 STAVNLKNASLKISKAMGVDVPQENGNHNLSSNAGPSSPRAFNLDADNTASVXXXXXXXX 480

Query: 481 ------------------PFSKTNLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 540
                                    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 540

Query: 541 XXXXXXXXXNCNPESSTSSFVEKPQEKFIGQWFPRDKKDEMVLNLLPRVQELQNQLQEWT 600
           XXXXXXX  +C  +++ S F   P +K  GQW PRDKKDEM+L L+PRV++LQNQLQEWT
Sbjct: 541 XXXXXXXSLSCKSDATNSIFSGIPYDKSSGQWVPRDKKDEMILKLVPRVRDLQNQLQEWT 600

Query: 601 QWANQKVMQAARRLSKDKAEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 660
           +WANQKVMQAARRLSKDKAE XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 601 EWANQKVMQAARRLSKDKAELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 660

Query: 661 XXXXXXXXXXXXXXXXXXXXXXDMEVAKLRATESAASYQEVSKREKKTLMKVQSWEKQKM 720
           XXXXXXXXXXXXXXXXXXXXXX+ME AK+RA ESAAS QEVSKREKKTLMK+QSWEKQK+
Sbjct: 661 XXXXXXXXXXXXXXXXXXXXXXEMEAAKVRAAESAASCQEVSKREKKTLMKIQSWEKQKV 720

Query: 721 LFQEEHTAEKRKVKKLIQELEQARDLQEQLEGRWKLEERAKDELLVQAASLRKEREQIED 780
           L  EE   EKRK K+L+QE+EQA+DLQEQLE RW+ EE +KDELL QA+S+RKER     
Sbjct: 721 LLNEELVIEKRKFKQLLQEVEQAKDLQEQLEARWQQEETSKDELLEQASSVRKERXXXXA 780

Query: 781 SVKVKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRIAALKRGIDGSYASRLTD 840
           S K K             XXXXXXXXXXXXXXXXXX    S+IAAL+RGIDGSYAS++TD
Sbjct: 781 STKSKEDMIKLKAENNLQXXXXXXXXXXXXXXXXXXKSDSSKIAALRRGIDGSYASKVTD 840

Query: 841 TRNNTDHKESWSPNVSESMKDLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTT 900
             N  D K S +P +SE +KD + YS TGGVKRERECVMCLSEEMSVVFLPCAHQVVC T
Sbjct: 841 IENGIDQKGSRTPYISEVVKDFHDYSETGGVKRERECVMCLSEEMSVVFLPCAHQVVCRT 900

Query: 901 CNELHEKQGMKDCPSCRSPIQRRIPVRYARS 902
           CNELHEKQGMKDCPSCRSPIQ RI VRYARS
Sbjct: 901 CNELHEKQGMKDCPSCRSPIQWRISVRYARS 930

BLAST of CsaV3_3G013920 vs. TrEMBL
Match: tr|A0A2I4F374|A0A2I4F374_9ROSI (putative E3 ubiquitin-protein ligase RF298 OS=Juglans regia OX=51240 GN=LOC108995056 PE=4 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 6.7e-247
Identity = 602/902 (66.74%), Postives = 689/902 (76.39%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
           M SMVAK +C S+S    +S++ QEKGSRNKRK+RADPP+ D N I +S Q +CPSYEFS
Sbjct: 1   MTSMVAK-ACSSSSCQVSASVSFQEKGSRNKRKFRADPPVDDQNNIVTSPQIECPSYEFS 60

Query: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNG-GSSDVGINWPRGELEVDEDQD 120
            EKFEI++  GQ S CD CS++Q  S GLKLDLGLS+  GS +VG + PR E+E DE QD
Sbjct: 61  TEKFEITTDHGQPSACDHCSVNQNHSDGLKLDLGLSSAIGSFEVGPSRPREEIEADEFQD 120

Query: 121 ADWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNV 180
           ADWSDLTEAQLEELVL NLD IFK AIKKIVA  YTEEVA KAV RSG+C+G KDTV+N+
Sbjct: 121 ADWSDLTEAQLEELVLGNLDAIFKSAIKKIVAYDYTEEVATKAVLRSGLCYGCKDTVTNI 180

Query: 181 VDNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISD 240
           VDN LAFLR GQEID SREHYF DLQQLEKY+LAELVCVLRE+RPFFSTGDAMWCLLI D
Sbjct: 181 VDNALAFLRSGQEIDPSREHYFVDLQQLEKYVLAELVCVLREVRPFFSTGDAMWCLLICD 240

Query: 241 MSVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHG 300
           M+V+ ACAMD D  ++ VCDG+S+ S +++ PQLK E KS E+NLP P+K   P +C+H 
Sbjct: 241 MNVSHACAMDGDHLSSFVCDGSSSSSHNSSQPQLKTETKSLELNLPSPLK---PFTCSHT 300

Query: 301 SQYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIE 360
           ++ + P   GVP+I+KPK  L  +G +SE E   +T + V++SF V+   Q+   EEK  
Sbjct: 301 TESNVPTVAGVPNIAKPKSSLVLNGLVSENEGTTTTSNTVDKSFDVSETCQSPAVEEKFG 360

Query: 361 SSRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGST 420
            SRKVHS  TKREYMLRQK  H +K++RTYG+KGSSR GKL+G GGL+LDKKLKS++ ST
Sbjct: 361 GSRKVHSGGTKREYMLRQKPHHAEKHYRTYGSKGSSRGGKLSGFGGLILDKKLKSLADST 420

Query: 421 AVNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLXXXX 480
             N KNASLKISK MG+DV  DNG H+LST   PSSS+  NL              XXXX
Sbjct: 421 TANLKNASLKISKPMGVDVPLDNGIHSLSTNSRPSSSVASNLXXXXXXXXXXXXXXXXXX 480

Query: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNCNPESSTSSFVEKPQEKFI 540
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX    E    S+   P +K +
Sbjct: 481 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAEPPNCSYAGIPYDKSL 540

Query: 541 GQWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAEXXXXXXXXX 600
           GQW PRD+KDEM+L L+PRV+ELQNQLQEWT+WANQKVMQAA RL KDKAE   XXXXXX
Sbjct: 541 GQWIPRDRKDEMILKLVPRVRELQNQLQEWTEWANQKVMQAAHRLGKDKAELKTXXXXXX 600

Query: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDMEVAKL 660
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX+ME AK 
Sbjct: 601 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMEAAKF 660

Query: 661 RATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQ 720
           RA ES AS QEVSKREKKTLMK Q+WEKQK +FQEE   EKRKVK+L+QELEQA+DL+  
Sbjct: 661 RAAESDASCQEVSKREKKTLMKFQTWEKQKTVFQEELMTEKRKVKQLLQELEQAKDLEXX 720

Query: 721 LEGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKXXXXXXXXXXXXXXXXXXXXXXXX 780
            E RWK EE+AK+ LL+QA+S+RKERE+IE   K K                        
Sbjct: 721 SEDRWKQEEKAKEGLLMQASSIRKEREEIEALAKSKENMIKSNAEKNLQKYKDDIHKLEK 780

Query: 781 XXXXXXXXXXXSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTG 840
                      S+IAALKRGIDG  A+R  D +N+ D KESW P +SE + D   YSGT 
Sbjct: 781 EISQLRLKTDSSKIAALKRGIDGG-ANRFMDLKNSPDRKESWPPCISEMVGDFQNYSGT- 840

Query: 841 GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYA 900
           GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRI VRYA
Sbjct: 841 GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRISVRYA 896

Query: 901 RS 902
            S
Sbjct: 901 HS 896