CsaV3_2G031680 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: NP_001275523.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >CCG14217.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 721.5 bits (1861), Expect = 1.4e-204
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: XP_008445138.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 682.6 bits (1760), Expect = 7.3e-193
Identity = 329/351 (93.73%), Postives = 341/351 (97.15%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQT-VPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASE 60
           MADQEIPPPSQLVPL+F SVQT VPESHLWPN  ELSAA+KLDKRVSLPLIDLV+D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMA 120
           LLGRACEEWGMFQLINHG+S+TLIEEAEEETRRLFTLPA QKMKTLRSPGSLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILK 180
           GISKYHPKLMWHEGFTI+GSPINEAKKLWPNDYKRFCDVMEEYQR+MKGLGERIIRLILK
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVG 240
           FLGISEEEMMKLLTSTDESIGKP+MALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQV 
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 NNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAY 300
           +NGLQIFKDGTGWVPLSPMSGTL+VN+GDLLHILSNGRF SILHRVMIQENKEHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           FYDPPG+IYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKK G GLPAIKI
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 627.1 bits (1616), Expect = 3.6e-176
Identity = 296/350 (84.57%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE+PPPSQLVPLDF S+QTVPESHLWPN+ ELS AIKLD+R+SLP+ID + DGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQLINHG+ KTLI+EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMAG
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGERIIRLI KF
Sbjct: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L ISEEEMMKLLTSTDES GKP+MALRLNSYPPCPDP +VMGLAAHTDTSL TI+HQ  +
Sbjct: 181 LDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARH 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQ+FKDGTGWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRVMI ENKEHRFSLAYF
Sbjct: 241 DGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           YDPPG++YISPYCKPLS++PQ PLYR VNVKEYF IKAKK GKGL AIKI
Sbjct: 301 YDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: XP_023002882.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 523.5 bits (1347), Expect = 5.6e-145
Identity = 256/350 (73.14%), Postives = 290/350 (82.86%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE       +PLDF SVQTVPESHLWP++ ELS+ I LD+++SLP IDL+ DGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQL NHGISKTLI E EEE RRLFTLPA +KMK LRS   +  TGYG A 
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSADRV--TGYGTAV 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPK MWHEGFTILGSP N+AKKLWP+D KRFCDVMEEYQ +MKGL +R+IRLI KF
Sbjct: 121 ISKYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L ISEEE MKLL+ST +S GK + AL+LNSYPPCPDP QVMGLAAHTDTSL TI+HQV N
Sbjct: 181 LDISEEETMKLLSSTGDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQIFKD   WVPLSPM G+ +VN+GDLLHI+SNGRFPSILHRV IQ  K HRFSLAYF
Sbjct: 241 DGLQIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           Y PP  +YISP+CKPLS++PQ PLYR V+V+EYF +KAK  G+GL  IKI
Sbjct: 301 YYPPPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: XP_022951873.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 514.6 bits (1324), Expect = 2.6e-142
Identity = 252/350 (72.00%), Postives = 287/350 (82.00%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE       +PLDF SVQTVPESHLWP++ ELS+ I LD+++SLP IDL+ DGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQL NHGISKTLI E EEE RRLFTLP  +KMKTLRS   +  TGYG A 
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSADRV--TGYGTAV 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPK MWHEGFTILGSP ++AKKLWP+D KRFCDVMEEYQ EMK L +R+I+LI KF
Sbjct: 121 ISKYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L IS EE MKLL+S D+S GK + AL+LNSYPPCPDP QVMGLAAHTDTSL TI+HQ  N
Sbjct: 181 LDISVEETMKLLSSMDDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQIFKD  GWVPLSPM G+ +VN+GDLLHI+SNGRF SILHRV IQ  K HRFSLAYF
Sbjct: 241 DGLQIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           Y PP  +YISP+CKPLS++PQ PLYR V+VKEYF +KAK  G+GL  +KI
Sbjct: 301 YYPPPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

BLAST of CsaV3_2G031680 vs. TAIR10
Match: AT1G15550.1 (gibberellin 3-oxidase 1)

HSP 1 Score: 284.3 bits (726), Expect = 1.0e-76
Identity = 148/344 (43.02%), Postives = 213/344 (61.92%), Query Frame = 0

Query: 9   PSQLVPLDFTSVQTVPESHLWPNATEL---SAAIKLDKRVSLPLIDLVSDGASELLGRAC 68
           P   +P DFTS++ +P+S+ W    +L   +A        ++PLIDL    A+  +G AC
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHAC 76

Query: 69  EEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYH 128
             WG FQ+ NHG+   L+++ E  T  LF LP  +K+K+ RS   +  +GYG+A I+ + 
Sbjct: 77  RTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGV--SGYGVARIASFF 136

Query: 129 PKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISE 188
            K MW EGFTI GSP+N+ +KLWP  +  +CD++EEY+  MK L  +++ L L  LG+SE
Sbjct: 137 NKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSE 196

Query: 189 EEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQI 248
           E++     S+D  +     AL+LN YP CP+P + MGLAAHTD++L TIL+Q    GLQ+
Sbjct: 197 EDIEWASLSSD--LNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQV 256

Query: 249 FKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPG 308
           F+D  GWV + P  G+L+VN+GDL HILSNG F S+LHR  + + +  R S+A+ + P  
Sbjct: 257 FRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQS 316

Query: 309 EIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIK 350
           +I ISP  K +S   + PLY+SV  KEY   KA    K L  I+
Sbjct: 317 DIKISPVPKLVSPV-ESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of CsaV3_2G031680 vs. TAIR10
Match: AT1G80340.1 (gibberellin 3-oxidase 2)

HSP 1 Score: 278.1 bits (710), Expect = 7.5e-75
Identity = 143/337 (42.43%), Postives = 211/337 (62.61%), Query Frame = 0

Query: 14  PLDFTSVQTVPESHLW-PNATELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMFQ 73
           P DF S   +P+S+ W P    L +A   D+  +LPLIDL     + L+G AC  WG FQ
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 133
           + NHG+   L+++ E  T  LF LP  +K+K  RS   +  +GYG+A I+ +  K MW E
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGV--SGYGVARIASFFNKKMWSE 140

Query: 134 GFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEEMMKLL 193
           GFT++GSP+++ +KLWP+ + ++C+++EEY+  M+ L  +++   L  LG+ E+++    
Sbjct: 141 GFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAG 200

Query: 194 TSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTGW 253
            ++D         ++LN YP CP+P + MGLAAHTD++L TIL+Q    GLQ+F+D  GW
Sbjct: 201 PNSD--FQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGW 260

Query: 254 VPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPGEIYISPY 313
           V   P+ G+L+VN+GDLLHIL+NG FPS+LHR  +   +  RFS+AY + PP +I ISP 
Sbjct: 261 VTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPL 320

Query: 314 CKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIK 350
            K L D  Q PLY S+  K+Y A KA    + L  I+
Sbjct: 321 PK-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of CsaV3_2G031680 vs. TAIR10
Match: AT4G21690.1 (gibberellin 3-oxidase 3)

HSP 1 Score: 265.8 bits (678), Expect = 3.8e-71
Identity = 141/342 (41.23%), Postives = 209/342 (61.11%), Query Frame = 0

Query: 11  QLVPLDFTSVQTVPESHLW--PNATELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEW 70
           +++PLDFT+ +T+P+SH+W  P     S  I        P+I L +     LL +ACEEW
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEW 76

Query: 71  GMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKL 130
           G+F + +HG+S +L+   + + +RLF+LP  +K+  +RSP    +TGYG+  IS ++ KL
Sbjct: 77  GVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDE--STGYGVVRISMFYDKL 136

Query: 131 MWHEGFTILGSPI-NEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEE 190
           MW EGF+++GS +   A  LWP+D+  FC+VMEEYQ+ M  L  R+I +++  LG++ E+
Sbjct: 137 MWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHED 196

Query: 191 MMKLL-TSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQI- 250
           +  L+   T          L+LNSYP CPDP   MGLA HTD+SL TIL+Q    GL+I 
Sbjct: 197 LGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIE 256

Query: 251 --FKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDP 310
              ++G+ W+ + P+ G+L+V +GDL HI+SNG+F S +HR ++ +   HR S AYF  P
Sbjct: 257 SPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGP 316

Query: 311 PGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGL 346
           P  + I P     SD    P+YR +  +EY A KA    K L
Sbjct: 317 PKNLQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKAL 344

BLAST of CsaV3_2G031680 vs. TAIR10
Match: AT1G80330.1 (gibberellin 3-oxidase 4)

HSP 1 Score: 259.2 bits (661), Expect = 3.6e-69
Identity = 138/342 (40.35%), Postives = 208/342 (60.82%), Query Frame = 0

Query: 19  SVQTVPESHLW-----PNATELSAAIKLDK-RVSLPLIDLVSDGASELLGRACEEWGMFQ 78
           S+QT+PES  W      +    S+A+  D    S+P+IDL +   + L+G A + WG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 79  LINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 138
           + NHGIS+ L+++ E  ++ LF +P+ +K++   S   +  +GYG   IS +  K MW E
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGV--SGYGEPRISPFFEKKMWSE 134

Query: 139 GFTIL-GSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEE--EMM 198
           GFTI   S  N    LWP+D+ ++C +++EY  EM+ L  R++  IL  LG++ E  E  
Sbjct: 135 GFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWA 194

Query: 199 KLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDG 258
             L  +   +G+   A+RLN YP CP+P + MGLAAHTD+++ TILHQ    GLQ+F++ 
Sbjct: 195 HKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREE 254

Query: 259 TGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFY-DPPGEIY 318
           +GWV + P  G L+VNIGDL HILSNG+ PS++HR  +   +  R S+AY +  P G++ 
Sbjct: 255 SGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGGPAGDVQ 314

Query: 319 ISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           I+P  K L+   +  LYRS+  KEY  IK +   K + AI++
Sbjct: 315 IAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of CsaV3_2G031680 vs. TAIR10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 196.8 bits (499), Expect = 2.2e-50
Identity = 107/276 (38.77%), Postives = 153/276 (55.43%), Query Frame = 0

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           L +ACE+WG FQ+INHGI   ++E+ EE     F +P  +K K    PG  T  GYG A 
Sbjct: 78  LSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPG--TVQGYGQAF 137

Query: 121 ISKYHPKLMWHEGFTI-LGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILK 180
           I     KL W   F + +  P     KLWP+   RF + +E Y +E++ L +R+++ I  
Sbjct: 138 IFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLKYIAI 197

Query: 181 FLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVG 240
            LG+ EE         +E  G+   A+R+N YPPC  P  V+GL+ H+D S  T+L Q  
Sbjct: 198 SLGLKEERF-------EEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSK 257

Query: 241 NN--GLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSL 300
           N+  GLQI KD T WVP+ P+   L++NIGD + +LSNG++ S+ HR +    KE R ++
Sbjct: 258 NSCVGLQILKDNT-WVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKE-RLTI 317

Query: 301 AYFYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEY 334
             FY P  E+ I P  + + D      YRS N  +Y
Sbjct: 318 VTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDY 342

BLAST of CsaV3_2G031680 vs. Swiss-Prot
Match: sp|O24648|G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.2e-83
Identity = 151/339 (44.54%), Postives = 222/339 (65.49%), Query Frame = 0

Query: 16  DFTSVQTVPESHLWPNATEL----SAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMF 75
           DF S+Q +PES+ W +  +     S  I  +   ++P+IDL    AS+L+G AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  QLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWH 135
           Q++NHGI  +L+E+ +   + LF+LP+ QK K  RSP  +  +GYG+A IS + PKLMW+
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGV--SGYGIARISSFFPKLMWY 140

Query: 136 EGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEEMMKL 195
           EGFTI+GSP++  ++LWP DY RFCD++ +Y   MK L   ++ L+L  LGI++E++   
Sbjct: 141 EGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDIK-- 200

Query: 196 LTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTG 255
              +     K   AL+LNSYP CPDP   MGLA HTD++  TIL Q   +GLQ+ ++G+G
Sbjct: 201 WAGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSG 260

Query: 256 WVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPGEIYISP 315
           W+ + P+ G L+VN+GDL HILSNG +PS+LHRV++   ++ RFS+AY Y PP  + I P
Sbjct: 261 WITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQ-RFSVAYLYGPPSNVEICP 320

Query: 316 YCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           + K +  T   PLYRSV   EY   KAK   K L ++++
Sbjct: 321 HAKLIGPTKP-PLYRSVTWNEYLGTKAKHFNKALSSVRL 353

BLAST of CsaV3_2G031680 vs. Swiss-Prot
Match: sp|Q39103|G3OX1_ARATH (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 284.3 bits (726), Expect = 1.9e-75
Identity = 148/344 (43.02%), Postives = 213/344 (61.92%), Query Frame = 0

Query: 9   PSQLVPLDFTSVQTVPESHLWPNATEL---SAAIKLDKRVSLPLIDLVSDGASELLGRAC 68
           P   +P DFTS++ +P+S+ W    +L   +A        ++PLIDL    A+  +G AC
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHAC 76

Query: 69  EEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYH 128
             WG FQ+ NHG+   L+++ E  T  LF LP  +K+K+ RS   +  +GYG+A I+ + 
Sbjct: 77  RTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGV--SGYGVARIASFF 136

Query: 129 PKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISE 188
            K MW EGFTI GSP+N+ +KLWP  +  +CD++EEY+  MK L  +++ L L  LG+SE
Sbjct: 137 NKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSE 196

Query: 189 EEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQI 248
           E++     S+D  +     AL+LN YP CP+P + MGLAAHTD++L TIL+Q    GLQ+
Sbjct: 197 EDIEWASLSSD--LNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQV 256

Query: 249 FKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPG 308
           F+D  GWV + P  G+L+VN+GDL HILSNG F S+LHR  + + +  R S+A+ + P  
Sbjct: 257 FRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQS 316

Query: 309 EIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIK 350
           +I ISP  K +S   + PLY+SV  KEY   KA    K L  I+
Sbjct: 317 DIKISPVPKLVSPV-ESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of CsaV3_2G031680 vs. Swiss-Prot
Match: sp|Q9ZT84|G3OX2_ARATH (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-73
Identity = 143/337 (42.43%), Postives = 211/337 (62.61%), Query Frame = 0

Query: 14  PLDFTSVQTVPESHLW-PNATELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMFQ 73
           P DF S   +P+S+ W P    L +A   D+  +LPLIDL     + L+G AC  WG FQ
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 133
           + NHG+   L+++ E  T  LF LP  +K+K  RS   +  +GYG+A I+ +  K MW E
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGV--SGYGVARIASFFNKKMWSE 140

Query: 134 GFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEEMMKLL 193
           GFT++GSP+++ +KLWP+ + ++C+++EEY+  M+ L  +++   L  LG+ E+++    
Sbjct: 141 GFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAG 200

Query: 194 TSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTGW 253
            ++D         ++LN YP CP+P + MGLAAHTD++L TIL+Q    GLQ+F+D  GW
Sbjct: 201 PNSD--FQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGW 260

Query: 254 VPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPGEIYISPY 313
           V   P+ G+L+VN+GDLLHIL+NG FPS+LHR  +   +  RFS+AY + PP +I ISP 
Sbjct: 261 VTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPL 320

Query: 314 CKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIK 350
            K L D  Q PLY S+  K+Y A KA    + L  I+
Sbjct: 321 PK-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of CsaV3_2G031680 vs. Swiss-Prot
Match: sp|Q9SVS8|G3OX3_ARATH (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.9e-70
Identity = 141/342 (41.23%), Postives = 209/342 (61.11%), Query Frame = 0

Query: 11  QLVPLDFTSVQTVPESHLW--PNATELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEW 70
           +++PLDFT+ +T+P+SH+W  P     S  I        P+I L +     LL +ACEEW
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEW 76

Query: 71  GMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKL 130
           G+F + +HG+S +L+   + + +RLF+LP  +K+  +RSP    +TGYG+  IS ++ KL
Sbjct: 77  GVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDE--STGYGVVRISMFYDKL 136

Query: 131 MWHEGFTILGSPI-NEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEE 190
           MW EGF+++GS +   A  LWP+D+  FC+VMEEYQ+ M  L  R+I +++  LG++ E+
Sbjct: 137 MWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHED 196

Query: 191 MMKLL-TSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQI- 250
           +  L+   T          L+LNSYP CPDP   MGLA HTD+SL TIL+Q    GL+I 
Sbjct: 197 LGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIE 256

Query: 251 --FKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDP 310
              ++G+ W+ + P+ G+L+V +GDL HI+SNG+F S +HR ++ +   HR S AYF  P
Sbjct: 257 SPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGP 316

Query: 311 PGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGL 346
           P  + I P     SD    P+YR +  +EY A KA    K L
Sbjct: 317 PKNLQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKAL 344

BLAST of CsaV3_2G031680 vs. Swiss-Prot
Match: sp|Q9C971|G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.5e-68
Identity = 138/342 (40.35%), Postives = 208/342 (60.82%), Query Frame = 0

Query: 19  SVQTVPESHLW-----PNATELSAAIKLDK-RVSLPLIDLVSDGASELLGRACEEWGMFQ 78
           S+QT+PES  W      +    S+A+  D    S+P+IDL +   + L+G A + WG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 79  LINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 138
           + NHGIS+ L+++ E  ++ LF +P+ +K++   S   +  +GYG   IS +  K MW E
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGV--SGYGEPRISPFFEKKMWSE 134

Query: 139 GFTIL-GSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEE--EMM 198
           GFTI   S  N    LWP+D+ ++C +++EY  EM+ L  R++  IL  LG++ E  E  
Sbjct: 135 GFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWA 194

Query: 199 KLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDG 258
             L  +   +G+   A+RLN YP CP+P + MGLAAHTD+++ TILHQ    GLQ+F++ 
Sbjct: 195 HKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREE 254

Query: 259 TGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFY-DPPGEIY 318
           +GWV + P  G L+VNIGDL HILSNG+ PS++HR  +   +  R S+AY +  P G++ 
Sbjct: 255 SGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGGPAGDVQ 314

Query: 319 ISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           I+P  K L+   +  LYRS+  KEY  IK +   K + AI++
Sbjct: 315 IAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of CsaV3_2G031680 vs. TrEMBL
Match: tr|S0BE47|S0BE47_CUCSA (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 9.3e-205
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

BLAST of CsaV3_2G031680 vs. TrEMBL
Match: tr|A0A1S3BBH9|A0A1S3BBH9_CUCME (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 4.8e-193
Identity = 329/351 (93.73%), Postives = 341/351 (97.15%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQT-VPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASE 60
           MADQEIPPPSQLVPL+F SVQT VPESHLWPN  ELSAA+KLDKRVSLPLIDLV+D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMA 120
           LLGRACEEWGMFQLINHG+S+TLIEEAEEETRRLFTLPA QKMKTLRSPGSLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILK 180
           GISKYHPKLMWHEGFTI+GSPINEAKKLWPNDYKRFCDVMEEYQR+MKGLGERIIRLILK
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVG 240
           FLGISEEEMMKLLTSTDESIGKP+MALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQV 
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 NNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAY 300
           +NGLQIFKDGTGWVPLSPMSGTL+VN+GDLLHILSNGRF SILHRVMIQENKEHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           FYDPPG+IYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKK G GLPAIKI
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

BLAST of CsaV3_2G031680 vs. TrEMBL
Match: tr|Q9LM06|Q9LM06_CITLA (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 2.4e-176
Identity = 296/350 (84.57%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE+PPPSQLVPLDF S+QTVPESHLWPN+ ELS AIKLD+R+SLP+ID + DGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQLINHG+ KTLI+EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMAG
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGERIIRLI KF
Sbjct: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L ISEEEMMKLLTSTDES GKP+MALRLNSYPPCPDP +VMGLAAHTDTSL TI+HQ  +
Sbjct: 181 LDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARH 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQ+FKDGTGWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRVMI ENKEHRFSLAYF
Sbjct: 241 DGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           YDPPG++YISPYCKPLS++PQ PLYR VNVKEYF IKAKK GKGL AIKI
Sbjct: 301 YDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of CsaV3_2G031680 vs. TrEMBL
Match: tr|E1AXG4|E1AXG4_9ROSI (Gibberellin 3-oxidase 1 (Fragment) OS=Marah macrocarpa OX=48242 PE=2 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 1.7e-134
Identity = 239/355 (67.32%), Postives = 287/355 (80.85%), Query Frame = 0

Query: 1   MADQEI---PPPSQLVPLDFTSV-QTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSD- 60
           MADQEI   PP S LVPLDF+S  +TVPESH+W ++ ELS A+ LD+++SLP+IDL+ D 
Sbjct: 1   MADQEITTAPPSSPLVPLDFSSSHETVPESHIWVDSIELSPAMDLDEKLSLPVIDLLDDT 60

Query: 61  GASELLGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATG 120
            ASEL+G+AC++WGMFQLINHG+ K++I E E+E RRLF LP TQKMKT   PG+   TG
Sbjct: 61  TASELIGKACQQWGMFQLINHGVPKSIIAETEDEARRLFALPTTQKMKTF-GPGN---TG 120

Query: 121 YGMAGISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIR 180
           YGM  +SKYH K MWHEGFTI GSP+++AKKLWP+DYKRFCDVMEEYQR+MKGL +R++R
Sbjct: 121 YGMVPLSKYHSKSMWHEGFTIFGSPLDDAKKLWPSDYKRFCDVMEEYQRKMKGLADRLMR 180

Query: 181 LILKFLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTIL 240
           LILKFL ISEEE+MKL+ + ++S  K + ALRLN YPPCPDP +V+G+A+HTDTS  TI+
Sbjct: 181 LILKFLDISEEEIMKLMFTPEDS-SKIYTALRLNLYPPCPDPDRVVGMASHTDTSFFTII 240

Query: 241 HQVGNNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRF 300
           HQ  N+GLQIFKD  GWVPLSP SGTL+VN+GDLL ILSNGRFPSILHRVMIQE  E R 
Sbjct: 241 HQARNDGLQIFKDEAGWVPLSPTSGTLMVNVGDLLQILSNGRFPSILHRVMIQEKMEDRL 300

Query: 301 SLAYFYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           SLAYFY PP  IYI+PYCKPLS++PQ PLYR V VKEY   K+    KGL  +KI
Sbjct: 301 SLAYFYTPPPHIYIAPYCKPLSESPQIPLYRCVTVKEYSTSKSNNNFKGLSTVKI 350

BLAST of CsaV3_2G031680 vs. TrEMBL
Match: tr|O24417|O24417_CUCMA (Gibberellin 2beta,3beta-hydroxylase OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.5e-122
Identity = 205/346 (59.25%), Postives = 263/346 (76.01%), Query Frame = 0

Query: 5   EIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASELLGRA 64
           ++P    L PLD  +V+ VPESH+WP+  E S   K DK VS+P++D   D   EL+G+A
Sbjct: 3   KLPAVGLLTPLDLNTVEAVPESHVWPHLDESSQKFKSDKTVSIPVVDFNDDNVLELIGKA 62

Query: 65  CEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKY 124
           CEEWGMFQLINHGI KTL  E EE  R LF LP +QKMKTL  PG  TA GY MA ++K+
Sbjct: 63  CEEWGMFQLINHGIPKTLTAETEEVARCLFALPKSQKMKTLNVPG--TANGYCMARLTKH 122

Query: 125 HPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGIS 184
           H K+MWHEGFT++GSP+++ KKLWP+DY+ FCD +EEYQ +MK L +++I LI K+LGIS
Sbjct: 123 HDKMMWHEGFTVIGSPVDDFKKLWPSDYQPFCDKVEEYQLKMKALADKLISLIFKYLGIS 182

Query: 185 EEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQ 244
           +EEM+K L+  D + GKPH+ALRLNS+PPCP+P +VMGLAAHTDTSL T+LHQ    GLQ
Sbjct: 183 DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVMGLAAHTDTSLFTMLHQARREGLQ 242

Query: 245 IFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPP 304
           I  +  GW+PL+P    LI+NIGD L I+SNGRF S+ HRVMI+E ++   S+AYF+ PP
Sbjct: 243 ILNEKEGWLPLAPRRDALIINIGDFLQIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPP 302

Query: 305 GEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           G +Y++PYCKPLS+T Q P+Y+ VNVKEYF IKAK +GKG+ A+ I
Sbjct: 303 GHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKASGKGIAALTI 346