CsaV3_2G010960 (gene) Cucumber (Chinese Long) v3

NameCsaV3_2G010960
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
DescriptionMechanosensitive ion channel protein
Locationchr2 : 8198351 .. 8208145 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTTCTATCTCGCCTAATCTCTATGGTAAGTTGGGTGTCACGCTTGCTCATCTTGTTTAGTCTTGTTGGTGTGATTCAATATCGTGGGTTTTGGTTGGTGTGGCTCATGTCCATATTGGAGAAGAAGAGCACGAGTAGAGTAGAGAAAGAAGATGTGTGGGAGATGAGAAAGAATAGTAAAAAAGAAATGATGAATATTGTTGTAATTGAAGTAAGGATTTGGAGTTTGATTTTCTAAAAAATGTGTACCAAACTTAAGAAGAAGAGGAAGGTGAATAAAATTGAATATCTATTAGTATTAATAAAATAATATTGAAAAAGAAAAAGGGGAAAATGAGAAATAAAATCACCTCAATGCGGCTGCTTCGTCGTCTTCACATTCAATCATTTCATTTTTGCCTTTTAAACCACATTTTTTATTTTTTATATTTTTTTATATTTTTACAAAATGTTCCTTAAGCTCCATGGCGTTTCACATTTCCCTCTCTTAAATCCACCTATTATAAATTTCCATTGCCCACTTCTTCACTTCACTACACACCTCAATCCCCCCATTCATTCATGGACGTCCGAAAGTCAGATAATGACCATCTAGTCCTCACCATCGACCCACCGGACAAGGAACAAATTTCACCCTCTCCCACCACCACCCCCACCCCCACCAGAACCAAAACCCTCCGTCGTCTCAGAACTAAACCAAAATCCCGCTTCGATGAACCTAACTACCCTCTTTCCACTCCCAAAACCATCCCTGAATCTACAGATCTCCTTCAACCTCCGCTACAACAAGAAGACTCAACTTCTTCATCTTCATCTTCTTCCTCTTCCTCCGACTACGAAGACGGAGAAATCGGACCGGAAAATGAGAACGAACGTAAAGCCGGACGAAGACGGAGAAGGAAGGGTAAGCGGAAGAAGATCAATAAACGAGTGTTAATTGAATGGATATTGTTCCTCACCATAACCACTTGCTTAATCTGTGCTCTAACACTTGAATCGCTTCAAGAGAAGCAGATTTGGAGCTTAGAGGTCTGGAAGTGGTGCTTAATCGTAATGGTGGTTTTCTGTGGACGTTTGGTTTCGGAATGGCTTGTCGGAGTCCTTGTCTTCGTAATCGAACGAAACTTCATGCTCCGTGAACGAGTCTTGTATTTCGTCTACGGTCTCCGCAAGAGCTTCCAGAACTGCGCTTGGTTGGGGCTAGTTCTAATCGCTTGGATGATAATGTTCCCGGACGTTCACCACAACAATAAAGTCCTCCTAAAGGTTTTCCGATTCTTAATCGCCGTCCTAATCGGCGCCACCATCTGGCTTCTCAAGATCCTTCTCGTGAAAGTTCTTGCATCCTCGTTCCACGTCGCGACGTTCTTCGATCGAATGAAGGAGAGCGTGTTCAACCACTACATCCTCGAGACACTATCCGGACCGCCACTGGACGAGGAGGAGAGGGATAAGGAAGTAAATCGTCGGCGGCGGTTGGTACATATGTCGAAATCACTGCCAGCGCGGTGGCGGGAGGGCGGAGGAGGACAGACTTTGTCGAGATCGAAACGGCAGGATTCTTGTCAGAAGATCGATATGGAGAGATTGAGGAAGCTGAGCTTGGAGAGGAGACCGTCGGCTTGGAGTGTGAAAAGATTGGTGAGCTATGTGAGATCGTCGGGACTGTCGACGATATCGAGGACGGTGGATGATTTTGCCAACGCCGAATCGGAGATTACAAGTGAATCGGAGGCGAGAAACTGCGCTCAGAGAGTGTTCAAGAATGTCGCTAAGCCGGGTGCCAGGTTCCTCTCTCTCTCTCTTTCTCTCTCTACAATACAATTATATTTTATATTATTTCTTTTTAACATTTTAATCATTTTTTCCAAATGGTTTAATTCAATGTTTTATTTATTAACCAATTTAGTGCCTCCAGTTGGTCTTCCTTTTATTCAAAATACTCTCAAATCCTAATATTTAAATCCTTACTAAAATGAAGTGTGGCAGGCAGTCTGTTCAACTTCATTTTCTTTGCAAATTCATACTTAAAAAGATAAATGTTTAAATTACATGGCACCCATTTTTTCTTTTCTTTTTTTTTTGTCTCCGCAGCAATCATTCTTTTTTAGCACATACTTTTAATTATTAATAAATTAATACACCTTTATGTATGTAACACACGTATATAATATAGTTTAACATAAATTTCTAGGTTTACCTCCTTTTAAATGATTTTAGTATTCGATATGATGATATGTATATACTTGTTTCTTTTCCCTTCCTCTCCTTTTATCTTTTTTTTTTTTTTTTTGGAAAAAGTGAATTTTGGTTGTGCCCACAAATGAAAAGAAAAAATGTAGTATCATATCTTAAGTCATTGCCCCAATTAAAATATCTTTCATTCACAATGCTACTATTCGTACCATATTTTGTAGAGTATGGGAATTCACCATTTTCACAATTTTTTAAATATTGCTCCACTTGATAACAACAAAAAATGCCCATGTTTCATTTACATAACATTGAAAAACTATTTTCTAAGTACCATTAAAGTTTAGCTAAGATCTTTTAATCATTTTAAATTAATTCTAAGGTTTTCTTTCTACTCCCCAATCCAGCACTTTGCTTCTATCTGAGATGTAAGAGGCATATAAAATATGAACGTACATGTGGCTTTCTCTCCTTTTTTCTTTTTTCTTTTTTCAGGATAGGTCGAGTGTGGGGTTTGCTTGTTTTTTTTTTCATTATGCTTTATTCATTTAAAAAGGGAAAAAAAATCTATGCCCCACCAAATTATTCCAAGTCTCTATAATTGAAAACATAAATCTGGCATAACCCAAATTGAATCATGTTGCCATTGCTTTTTTGTGAACGTAATTTTTTCTCTTAAATTTCTATTTTAAAGAATTAAAACCCAAAAAAGAACCAAAGTATTTCTAGTTATTTATTTATTGTAGGCAAACAAACTACGCTCTAAAGTATTTATGGATTTTTTTAAAAAAATAATGTTTTAAATCAGGCCAATTACTATTTTCCTATATATAAAGTTGTTAAAGAGTGTAGTTTTATGCTAGGTTTATAAAATAGGTCCTACGGATATGGTGATTGGTCAACATAAAAAGTATTTGAAAATCTCTCATTCTTTTGTGCGGACCTACACGTAGGACCGCGCAGGACACAACTGTCCTTGTTCACAAGGTAAGTTACTTTGGCCCGGTGTTGTCCTGTAGGTATTGCATCCTTTGATTCAATTTTTTTCTAAAAATAGAAAAGAGGTTTAAAATTTAAATAACAATTGGTTATGTATGTGTCTAATAAAAATGATTAAATATTTGGATGGATAAAAATTGATGGATTTCTATGCTGTCGTCTATCAAAGTATACTAATGTAAGAGACTTGAAATCACACAAACTAAGATCAAAATAAGTCTCATAATATTATGTCTAATCAAAACTTATATTTTAAACTATTATATTTTGTTTTGTTTTAACGTTTTAAATGAAATGAAATAAATAAATAATTCCACAAAACTTTGTAGGATATTTGCTGTGATGGATGCACAATTTCATACATGGAAAATATATCTTCTTTCCATTCTACTTTGAAAAACGGAATGAAACTTTCAAACTCGTCATTTTTATAATTATCAGGGTTAAATAAATAAATACGTATATATGCGTATTTTTTATTTCAACTTCTTAATTTCATAACTTTTTTTTTTTTTTGCATTTTTTGAATCTAATTATTTTTTGTTTCACTATCCATTAAAATTTTAAAAGAAAACAAAAGTAGGCTTAATCCTTTTTCAGTAGTAGATTGAGAAAAAGAACTATCCACCTTCAAGACTATATTTATTGACATTTGAATAAAAGATTACCTTTTCTTTATTTTCTTTTTTTAAAAAATAATTAAATAGTCAACCATTAAAAATATTTATAAAATATAATAAAATTTTATTTACTAGCACTGCAGATTTGAATAATAAGTTTTATCTATGTTTAGGTGTATATTTGAAAATTTTCCAAAATAATCCTAACAAAAGACAAACAAAATTTAGAAGGGTGGAGTAGAAGTTAGAAGATAGGTAAAATATACTGGAACCAAACAAATTGAGTTCCTATAAAAGCATATCAAGATATCCACAGATATTCTGGATCCAAATTCCATCACTGTCATTAGATGTGTTGATTAGAAAAGACAGCTTAGCAGCTCTAAAATGAAGTATATGTCCTTGTCTTTTGACAGTACAATGCACAATGACTTTAATCTGTCCTCTTCCTGCCAAAACAACAACAATAATAATAAAATCAAAGAAACAGAAAAAAGTAGATTCAAACTGTACAAAACTTTAGTTGTCCACTGGTTCATAGATTAATAAGAAAAAAACTTGGGGATGCACCTGTGTTGCTGCATCTCCCACGTGGACATTTTTTACCTTTTTCCAATTAATTTTTTTAACTATTTTTCAGGTAGATTGTGAAAAACGCAGCTTAATGTGCATCTAATTATTGCTTAATTAATTATAAAACTAAATTTTCTTTAGTAAAAATTATACAAACCATTGAAATTTAAGAACACGTAGTTGGCTTAAATTGATGAGAGAAAAAAATGCACCAAAGAAAAACTATTTTAAACATTGTAAACACTTCAGTTTTTTTTTTAAAAGTGACTAAATTTTAAAGTTAATCAATTAGAAAGTTAATTCAAACCTTTTGTAATTTAGTAAGATAATAATAAAAACCTTTCAGAAGAGAAGTTAAGGGTTGTAAATGGTTTTATTGTTTCATCAAGTGGAACAAGACTAATTAGAAAAATAATGATGAGTGCAGCACATGGCACTACTTCATTGAGTTTAGCTGGGGATGCCATTGCAATCGATAGAAGATGCGTACATCCCGGCTGCACCTGAATAAAAGTCTAAATAACTGAGCGTTTCTTATTTACATTTTTATGAGTGTTTCTTTTATTTAATTTTCTATATGTTTGATTGATTGCAGATTAGTGTTTTCATTTGTTAACTAGTCTCTTTCTAGCTATAGTTCTAGATAATTAATCCATGCCAACTTTTTGACAAATGTGTTAACTAATTTTTTTTCTTACTTGTCTGATTATGATACTGGTGGTGGCTTACATATGCAACTTTAGAAAATTTAGCTATTACTCGAAAAAAAAATATTATAAATGACAAAACTGTTTAAAATATTTACAAAATATAACAAAATTTTTTAATATATTATATTATCTTAGTCATAAAATATAAAATTTTACTATATTTTATAAATATTTTGATTCATTCTATCCTAACTAGATTTGGTAAATTGAAGCTAAACCATATGCAATTAAGGTAAGCTATGTTGTTGACACATATAACCTTATGTTATATTTTTCAAATTTTTGTTTGAATAAAAAATAAATAAAAGCCTATCGAAATTGTATGTGGAGTCGAATAAAAAGAAATCATGTTGTCAGAGTCATTATATTTATTTTAATATGATTTAAGAAATATATTTATTATCAGAACCAACCGCATTTGAATGGGTCCACATGCCATTACTAGCTACTTTATTGGGTTTAGTTTAGAATTTTTCTCTATTATTTTATTATTATGATGGTGAAGGAAAAGACCCACGTTATTGTAAAAGAAAAGAAATGGAGATACGATACGATGGGAGTTTCCACTAATCTAGATGATTGTTTTTGTAGAGAATTATATTATAATGTGAGAGAAATATTATAATTTCTGAAGCAGTGAAGTTGACTAAAAAAAATTAAATAAAGGAATTTAAGAGTTAGCGTTTATTTTGTCAAATTTTCTCGCAAACTATGCTAGTTTATTTTTTGAATTTCTTCTATTATTTACCTGTAGTATTATATTAATAAAGGGTTGGTTGTAAAACTAATTGGTTATAACGTGTGTGCTCTCTTTATTTTTTAAGGTAAAATTCTAAACATATACTCCCAATATTGTGTAAGCTTGTTAGACTTAAAAAGTGTTTTATGTCATTAAAATTTCAGTCAATTTTATGTTTATTAAAATTGGCTTTAACTTGTGTCTAACTGATCTTCTAAAAAGAAAAACAGTGTAATTTTAACATGAAACACTTGGAAATAGAACTTACTTTCAAATTCATTTTGTAGCTTCTCAAAAAGAAAGAAACTGATCAATTTTCAAACAAGACTATAAAAATAAAAAGATGGTGGATTAAACTTGTAATTTAACTAGGTTGGAAAAACAACTGAAGCAATCTTTACTTTGTTTTGATGTTTTTACAGATACATTGAAGAGGAAGATCTACTGAGATTCCTGAAGGATGAAGAAGTGAACACCATTTTCCCTCTCTTTGAAGGCGCCATTGAAACCGGAAAAATCTCCAAATCCGCTTTCAGAAACTGGGTGGTTTGTAAACTATCTCCCCACATCCTAAATTCTTTCAACACACTTTTCCAAATGGTATAAGAATTTTGACATGTCCTTTGATGCAGGTTCATGCCTACATTGAGCGGAAGGCATTGGCACATTCTTTAAACGACACCAAAACCGCCGTCCAGCAGCTCCACAAGCTGGCCAGCGCAGTCGTCATTGTGATCATCATTGTCATTTCTCTTCTCGTCCTTGGCGTCGCCACAACTAAGGTCCTCTTCGTCATCACCTCTCAGCTTCTCCTTGTTGGATTCATGTTCCAAAACACCTGCAAAACCATCTTTGAATCAATCATCTTCGTCTTTGTCATGCATCCTTTTGATGTTGGCGATCGCTGCGTCATCGATGGCGTCCATGTAAGTATATGTTGTTAGGAATACGAATGATGTCTTAGAAGTTACAAGTTTATATGGAATTTAAAATGTTTTTTCTAGTCCTTTCCTTGCTATATAATTAGATGGAAAGTTTAAGAACCGATTAAAACAATATTGAAATTTAAGACTTGGTTTACTAGACATTTTATGAAGTTCAAAAATTTATACCAATCTAAAACTTAGTTGCCACCAGACTTAAAATTTAACGAATAAATTACTAAATAGATGTTAAGGAGGTTTGGGACATTAATTTTATTATAGTCTATAAGTTATATTAATATATATTTGAAACGTAGACTATTTAGAACTACTTGGAGTCATAATTGAATGAGTTTGTAGTGTAATCTAAAATTCAAGTTTGAATCAAGCCTTTTGAGTCAACTTGTTATATCAAACACATTTTGTTTCTACAATTCTCAATTAAACTTTTTTCTATTATTTTCATGTTTTACGGCTATTCTAATTTTTCCGTTAAATTTCTCACTCATCCCAACAACTCTTTATTTATAGTAATCTTTATTATATTTCAGCCACCCTTTCTTATTTCATTACATAAGGAGGGAAATAAATATATATATATATGATAGGTAGAGAATAGTATATACAGATTTGAAATTGTAATACAATTAACGATGGTAGTTTATAATAGATGAAAATACAACTACTATAATTGGTACCTCAAACATTGTAATACTATAATTTTAAGTTCTTAGTTTTGTTTGTATACATTCAGTCCACTGATTTTAAAATTTACGCTGTTACTCTTGCTTTTGAATACTCAATATTGATGCTTCCTGTAACCATTTTAAACCGCAATTTATAGGAATTGGGTGATTTCTTGTTTTCAGATGTTTGTAGAAGAGATGAATATTTTGTCGACGGTGTTCTTGAGATTCGACAATGAGAAGATTTACTATCCCAACTCGGTTCTGCTGACGAAGCCGATCAGCAATTTCAGAAGAAGTCCCGACATGTCGGATACAGTCGATTTCACCATTGACGTCTCCACTTCTTTTGACATCATCACAGCCCTCAGGAAGGCAATGCAAATGTAAGCATTCTCATCTTCCTTTGTTAAAAACGCATTACGTTTTAGGTTTTAGAGATGTTTACACATTACATTCTTATACCTATATCATACATGATAATCTGATTCATAATCATCACAATTATAATCTTGAGTTTTATACTCAACTGTTATACAAGTTAAAAACTTTTTTAACAAATACCTTGAAAGTACAAAGTTTAGTCTCTGGAAAAAAGCATTTGGTTGTTTGAACTAGTGACTTGCTAAACTTTCAATTTCGTGTGTAACACAAATTCTTAATTTCAATTTTGCAAAATAAATTTTGATCTATTTGACATTTTTAATTAGTCGTTATTTTAAGTTAATTTCAATTTTGCAAAATAAATTTTGATCTATTTGACATTTTTAATTAGTCGTTATTTTAAGAGTTCAAATCTCGTTACTAGATATAAATTATGTTTAATGTATGTTGTACTTTTTAAAAATCTTCAACAATATGGTGGTAGAATTGAATGTGCAGGAAGCTATTAGACATTTATACTATTTTTGGACATTTCAGAGTAATTAAAAGATTATTTACGTATTAGGAATCAAATCTTTTAAAAAATTGAGATATTAAATTTCAAGTTTTAACATTTTTTTTACCGAAGTTCCAATTGTTAAATTTGACTGGCCTACCCATTTAGAATTTTAATTTTTAGTTTATATTCATGTTTAACTTTACACGTTAGCCTAAATGTTTAGGTTTTTAGGTCATCATTAGTTCAAAATTTGTGCTGTTCACTTTTCCTTTCAATATCAATAGTTTCTTGTGTTGAACATTATATGAACACTTGAACCCACATGTGAGAGAATATAGAACAACTTCAATTTTTTATCCTCTTTGTTAATTCATATTATATTTAAAACATCATGATGTTATTTTTTTTTATTAGTTAAATTATTTTTTAGTTTTAGATTAAACCATCTTTTAAAATGATAATTGATGTCTTAATTTACTTTGCATAGCTCTAATACTTTCAATCAAATTAATTCAAACAAACAAAGCTATGTCTTTTCAATCAATTTTACCATAGTTATAACTATTAATTTTGGTTTTCTTACTTGAAAGTTTAATTGAAGGAAGAGTTAGAATTTACTCTATGATTTTGATAACACTTAATATGTGGTCTATGATTTCATTACTTCATGAATAATTCTTGTCATAGAGAATATTTAGTAGAGTTTCTATCAAACATATGGACTAGAATTTAAAATGTTTTTTTTAAGGTATAGGATCAATTCAAAATTGGGTTAATATATGTAAAATATTTTGCAGATACATTGAGAGCAAACCAAAGCATTGGAGCCCAAAACACTCATTGGTAGTGAAAGAGATTGAAAATGTGGACAAGATGAAGATGAGCTTGTGTGTGCAGCACACCATGAACCTTCAAAACTTCCCTGAAAGAAACAACAGAAGATCAGACCTCATTTTGGAACTGAAAAGGGTTTTTGAGAATCTGGGCATAAAATACCATCTCCTTCCCCAAGAAGTTCTTGTCACTCAGTTCAACTTGACTAATGGCAGAATGGCTATCCCGTCTTCATGAGAAACGACATTCATTTGCTGTACATTTATGATGTATGTATCAATTGCGTTGTGACAGCCACACGTTGAATATTTTCTTGTGTTGTCCAGAAAAATGAGCTAAATTAGTTCTTTCACCCTACGGGTTGGCTTTCACAAAGGTATGTGGAAAGTGAGTATTTGGAGGATTAGTATTATTGTTATACATAGAGTTTTGTAGTTTTGACTTCTAAGAAAAAATTGGAAATGTGAATTTTCAACAAGGAGAGTTGCTTCAGGGAGATATTCTCCCAATCCTTTGATTTTCTTTCATCCAGCTCCACTCAAATATATACGAATATAGGTATGTATATTTGTTGTGAGGAGATACAGATAACAGAAGAAAATGAAGTACATGTTAATAAACAAATACATAAA

mRNA sequence

ATGGACGTCCGAAAGTCAGATAATGACCATCTAGTCCTCACCATCGACCCACCGGACAAGGAACAAATTTCACCCTCTCCCACCACCACCCCCACCCCCACCAGAACCAAAACCCTCCGTCGTCTCAGAACTAAACCAAAATCCCGCTTCGATGAACCTAACTACCCTCTTTCCACTCCCAAAACCATCCCTGAATCTACAGATCTCCTTCAACCTCCGCTACAACAAGAAGACTCAACTTCTTCATCTTCATCTTCTTCCTCTTCCTCCGACTACGAAGACGGAGAAATCGGACCGGAAAATGAGAACGAACGTAAAGCCGGACGAAGACGGAGAAGGAAGGGTAAGCGGAAGAAGATCAATAAACGAGTGTTAATTGAATGGATATTGTTCCTCACCATAACCACTTGCTTAATCTGTGCTCTAACACTTGAATCGCTTCAAGAGAAGCAGATTTGGAGCTTAGAGGTCTGGAAGTGGTGCTTAATCGTAATGGTGGTTTTCTGTGGACGTTTGGTTTCGGAATGGCTTGTCGGAGTCCTTGTCTTCGTAATCGAACGAAACTTCATGCTCCGTGAACGAGTCTTGTATTTCGTCTACGGTCTCCGCAAGAGCTTCCAGAACTGCGCTTGGTTGGGGCTAGTTCTAATCGCTTGGATGATAATGTTCCCGGACGTTCACCACAACAATAAAGTCCTCCTAAAGGTTTTCCGATTCTTAATCGCCGTCCTAATCGGCGCCACCATCTGGCTTCTCAAGATCCTTCTCGTGAAAGTTCTTGCATCCTCGTTCCACGTCGCGACGTTCTTCGATCGAATGAAGGAGAGCGTGTTCAACCACTACATCCTCGAGACACTATCCGGACCGCCACTGGACGAGGAGGAGAGGGATAAGGAAGTAAATCGTCGGCGGCGGTTGGTACATATGTCGAAATCACTGCCAGCGCGGTGGCGGGAGGGCGGAGGAGGACAGACTTTGTCGAGATCGAAACGGCAGGATTCTTGTCAGAAGATCGATATGGAGAGATTGAGGAAGCTGAGCTTGGAGAGGAGACCGTCGGCTTGGAGTGTGAAAAGATTGGTGAGCTATGTGAGATCGTCGGGACTGTCGACGATATCGAGGACGGTGGATGATTTTGCCAACGCCGAATCGGAGATTACAAGTGAATCGGAGGCGAGAAACTGCGCTCAGAGAGTGTTCAAGAATGTCGCTAAGCCGGGTGCCAGATACATTGAAGAGGAAGATCTACTGAGATTCCTGAAGGATGAAGAAGTGAACACCATTTTCCCTCTCTTTGAAGGCGCCATTGAAACCGGAAAAATCTCCAAATCCGCTTTCAGAAACTGGGTGGTTCATGCCTACATTGAGCGGAAGGCATTGGCACATTCTTTAAACGACACCAAAACCGCCGTCCAGCAGCTCCACAAGCTGGCCAGCGCAGTCGTCATTGTGATCATCATTGTCATTTCTCTTCTCGTCCTTGGCGTCGCCACAACTAAGGTCCTCTTCGTCATCACCTCTCAGCTTCTCCTTGTTGGATTCATGTTCCAAAACACCTGCAAAACCATCTTTGAATCAATCATCTTCGTCTTTGTCATGCATCCTTTTGATGTTGGCGATCGCTGCGTCATCGATGGCGTCCATATGTTTGTAGAAGAGATGAATATTTTGTCGACGGTGTTCTTGAGATTCGACAATGAGAAGATTTACTATCCCAACTCGGTTCTGCTGACGAAGCCGATCAGCAATTTCAGAAGAAGTCCCGACATGTCGGATACAGTCGATTTCACCATTGACGTCTCCACTTCTTTTGACATCATCACAGCCCTCAGGAAGGCAATGCAAATATACATTGAGAGCAAACCAAAGCATTGGAGCCCAAAACACTCATTGGTAGTGAAAGAGATTGAAAATGTGGACAAGATGAAGATGAGCTTGTGTGTGCAGCACACCATGAACCTTCAAAACTTCCCTGAAAGAAACAACAGAAGATCAGACCTCATTTTGGAACTGAAAAGGGTTTTTGAGAATCTGGGCATAAAATACCATCTCCTTCCCCAAGAAGTTCTTGTCACTCAGTTCAACTTGACTAATGGCAGAATGGCTATCCCGTCTTCATGA

Coding sequence (CDS)

ATGGACGTCCGAAAGTCAGATAATGACCATCTAGTCCTCACCATCGACCCACCGGACAAGGAACAAATTTCACCCTCTCCCACCACCACCCCCACCCCCACCAGAACCAAAACCCTCCGTCGTCTCAGAACTAAACCAAAATCCCGCTTCGATGAACCTAACTACCCTCTTTCCACTCCCAAAACCATCCCTGAATCTACAGATCTCCTTCAACCTCCGCTACAACAAGAAGACTCAACTTCTTCATCTTCATCTTCTTCCTCTTCCTCCGACTACGAAGACGGAGAAATCGGACCGGAAAATGAGAACGAACGTAAAGCCGGACGAAGACGGAGAAGGAAGGGTAAGCGGAAGAAGATCAATAAACGAGTGTTAATTGAATGGATATTGTTCCTCACCATAACCACTTGCTTAATCTGTGCTCTAACACTTGAATCGCTTCAAGAGAAGCAGATTTGGAGCTTAGAGGTCTGGAAGTGGTGCTTAATCGTAATGGTGGTTTTCTGTGGACGTTTGGTTTCGGAATGGCTTGTCGGAGTCCTTGTCTTCGTAATCGAACGAAACTTCATGCTCCGTGAACGAGTCTTGTATTTCGTCTACGGTCTCCGCAAGAGCTTCCAGAACTGCGCTTGGTTGGGGCTAGTTCTAATCGCTTGGATGATAATGTTCCCGGACGTTCACCACAACAATAAAGTCCTCCTAAAGGTTTTCCGATTCTTAATCGCCGTCCTAATCGGCGCCACCATCTGGCTTCTCAAGATCCTTCTCGTGAAAGTTCTTGCATCCTCGTTCCACGTCGCGACGTTCTTCGATCGAATGAAGGAGAGCGTGTTCAACCACTACATCCTCGAGACACTATCCGGACCGCCACTGGACGAGGAGGAGAGGGATAAGGAAGTAAATCGTCGGCGGCGGTTGGTACATATGTCGAAATCACTGCCAGCGCGGTGGCGGGAGGGCGGAGGAGGACAGACTTTGTCGAGATCGAAACGGCAGGATTCTTGTCAGAAGATCGATATGGAGAGATTGAGGAAGCTGAGCTTGGAGAGGAGACCGTCGGCTTGGAGTGTGAAAAGATTGGTGAGCTATGTGAGATCGTCGGGACTGTCGACGATATCGAGGACGGTGGATGATTTTGCCAACGCCGAATCGGAGATTACAAGTGAATCGGAGGCGAGAAACTGCGCTCAGAGAGTGTTCAAGAATGTCGCTAAGCCGGGTGCCAGATACATTGAAGAGGAAGATCTACTGAGATTCCTGAAGGATGAAGAAGTGAACACCATTTTCCCTCTCTTTGAAGGCGCCATTGAAACCGGAAAAATCTCCAAATCCGCTTTCAGAAACTGGGTGGTTCATGCCTACATTGAGCGGAAGGCATTGGCACATTCTTTAAACGACACCAAAACCGCCGTCCAGCAGCTCCACAAGCTGGCCAGCGCAGTCGTCATTGTGATCATCATTGTCATTTCTCTTCTCGTCCTTGGCGTCGCCACAACTAAGGTCCTCTTCGTCATCACCTCTCAGCTTCTCCTTGTTGGATTCATGTTCCAAAACACCTGCAAAACCATCTTTGAATCAATCATCTTCGTCTTTGTCATGCATCCTTTTGATGTTGGCGATCGCTGCGTCATCGATGGCGTCCATATGTTTGTAGAAGAGATGAATATTTTGTCGACGGTGTTCTTGAGATTCGACAATGAGAAGATTTACTATCCCAACTCGGTTCTGCTGACGAAGCCGATCAGCAATTTCAGAAGAAGTCCCGACATGTCGGATACAGTCGATTTCACCATTGACGTCTCCACTTCTTTTGACATCATCACAGCCCTCAGGAAGGCAATGCAAATATACATTGAGAGCAAACCAAAGCATTGGAGCCCAAAACACTCATTGGTAGTGAAAGAGATTGAAAATGTGGACAAGATGAAGATGAGCTTGTGTGTGCAGCACACCATGAACCTTCAAAACTTCCCTGAAAGAAACAACAGAAGATCAGACCTCATTTTGGAACTGAAAAGGGTTTTTGAGAATCTGGGCATAAAATACCATCTCCTTCCCCAAGAAGTTCTTGTCACTCAGTTCAACTTGACTAATGGCAGAATGGCTATCCCGTCTTCATGA

Protein sequence

MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTPKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKINKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS
BLAST of CsaV3_2G010960 vs. NCBI nr
Match: XP_011649063.1 (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis sativus] >KGN61410.1 hypothetical protein Csa_2G118330 [Cucumis sativus])

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 706/706 (100.00%), Postives = 706/706 (100.00%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60
           MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP
Sbjct: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60

Query: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120
           KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI
Sbjct: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL
Sbjct: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV
Sbjct: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL
Sbjct: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL
Sbjct: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480
           KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX
Sbjct: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480

Query: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER
Sbjct: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS
Sbjct: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 706

BLAST of CsaV3_2G010960 vs. NCBI nr
Match: XP_008457175.1 (PREDICTED: mechanosensitive ion channel protein 10 [Cucumis melo])

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 680/706 (96.32%), Postives = 686/706 (97.17%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60
           M+ RKSDNDHLVLTIDPP KEQI  XXXXXXXXXXXX          SRFDEPNYPLSTP
Sbjct: 1   MEPRKSDNDHLVLTIDPPQKEQISPXXXXXXXXXXXXLRRLNFSKPKSRFDEPNYPLSTP 60

Query: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120
           +TIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI
Sbjct: 61  RTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTLES+QEKQIWSLEVWKWCLIVMV+FCGRLVSEWLVGV
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLESIQEKQIWSLEVWKWCLIVMVIFCGRLVSEWLVGV 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVFVIERNFMLRERVLYFVYGLRKSFQNCAWL LVLIAWMIMFPDVHH NKVLLKVFRFL
Sbjct: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLALVLIAWMIMFPDVHHKNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV
Sbjct: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NR R L+HMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL
Sbjct: 301 NRGRGLMHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL
Sbjct: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480
           K EEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX
Sbjct: 421 KVEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480

Query: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFD ITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER
Sbjct: 601 STSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFENLGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 706

BLAST of CsaV3_2G010960 vs. NCBI nr
Match: XP_022964525.1 (mechanosensitive ion channel protein 10-like [Cucurbita moschata])

HSP 1 Score: 1064.3 bits (2751), Expect = 1.8e-307
Identity = 621/706 (87.96%), Postives = 639/706 (90.51%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60
           M+VRK +NDHLVL IDPP+KEQI                        SRFDEPN+PLSTP
Sbjct: 1   MEVRKPENDHLVLAIDPPEKEQI-----SLSPARTKTLRRLNFSKPKSRFDEPNFPLSTP 60

Query: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120
           +TIPESTDLLQP            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 
Sbjct: 61  RTIPESTDLLQP--------TAEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTLESLQ KQIWSLEVWKWCLIVMVVFCGRLVSEWLVG+
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLESLQAKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGL 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVF+IERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVH+ NKVLLKVFRFL
Sbjct: 181 LVFIIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHNKNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVL+GATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDE E DKE 
Sbjct: 241 IAVLVGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEAEMDKEE 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NR+RRL  MSKSLPAR +E GGGQ LSRSKRQDSC+KIDME LRKLSL+RRPSAWS KRL
Sbjct: 301 NRKRRLQKMSKSLPARLQESGGGQILSRSKRQDSCKKIDMEMLRKLSLQRRPSAWSAKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           +SYV+SSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGAR I E+DLLRFL
Sbjct: 361 MSYVKSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARSIGEDDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480
           K EEV TIFPLFEGA+ETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX
Sbjct: 421 KVEEVTTIFPLFEGAMETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480

Query: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           XXXXXXXXXXXXXXXX         TSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 XXXXXXXXXXXXXXXXXXXXXXXXXTSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFD ITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMN QNFPER
Sbjct: 601 STSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNHQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFE LGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 NNRRSDLILELKRVFELLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 693

BLAST of CsaV3_2G010960 vs. NCBI nr
Match: XP_023513519.1 (mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1061.2 bits (2743), Expect = 1.5e-306
Identity = 619/706 (87.68%), Postives = 639/706 (90.51%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60
           M+VRK +NDHLVL IDPP+KEQI                        SRFDEPN+PLSTP
Sbjct: 1   MEVRKPENDHLVLAIDPPEKEQI-----SLSPARTKTLRRLNFSKPKSRFDEPNFPLSTP 60

Query: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120
           +TIPEST+LLQP            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 
Sbjct: 61  RTIPESTELLQP--------TAEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTL+SLQ KQIWSLEVWKWCLIVMVVFCGRLVSEWLVG+
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLKSLQAKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGL 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVF+IERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVH+ NKVLLKVFRFL
Sbjct: 181 LVFIIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHNKNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVL+GATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDE E DKE 
Sbjct: 241 IAVLVGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEAEMDKEE 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NR+RRL  MSKSLPAR +E GGGQ LSRSKRQDSC+KIDME LRKLSL+RRPSAWS KRL
Sbjct: 301 NRKRRLQKMSKSLPARLQESGGGQILSRSKRQDSCKKIDMEMLRKLSLQRRPSAWSAKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           +SYV+SSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGAR I E+DLLRFL
Sbjct: 361 MSYVKSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARSIGEDDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480
           K EEV TIFPLFEGA+ETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX
Sbjct: 421 KVEEVTTIFPLFEGAMETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480

Query: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           XXXXXXXXXXXXXXXX         TSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 XXXXXXXXXXXXXXXXXXXXXXXXXTSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFD ITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMN QNFPER
Sbjct: 601 STSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNHQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFE LGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 NNRRSDLILELKRVFELLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 693

BLAST of CsaV3_2G010960 vs. NCBI nr
Match: XP_023000433.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 1054.7 bits (2726), Expect = 1.4e-304
Identity = 621/706 (87.96%), Postives = 640/706 (90.65%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60
           M+VRK +NDHLVL ID P+KEQI                        SRFDEPN+PLSTP
Sbjct: 1   MEVRKPENDHLVLAIDTPEKEQI-----SLSPARTKTLRRLNFSKPKSRFDEPNFPLSTP 60

Query: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120
           +TIPES DL QP        XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 
Sbjct: 61  RTIPESADLPQP--------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTLESLQ KQIWSLEVWKWCLIVMVVFCGRLVSEWLVG+
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLESLQAKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGL 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVF+IERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVH+ NKVLLKVFRFL
Sbjct: 181 LVFIIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHNKNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVL+GATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDE E DKE 
Sbjct: 241 IAVLVGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEGEMDKEE 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NR+RRL  MSKSLPAR +EGGGGQ LSRSKRQDSC+KIDME LRKLSL+RRPSAWS KRL
Sbjct: 301 NRKRRLQKMSKSLPARLQEGGGGQILSRSKRQDSCKKIDMEMLRKLSLQRRPSAWSAKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           + YV+SSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGAR I E+DLLRFL
Sbjct: 361 MIYVKSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARSIGEDDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480
           K EEV TIFPLFEGA+ETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX
Sbjct: 421 KVEEVTTIFPLFEGAMETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480

Query: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           XXXXXXXXXXXXXXXX         TSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 XXXXXXXXXXXXXXXXXXXXXXXXXTSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFD ITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMN QNFPER
Sbjct: 601 STSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNHQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFE LGIKYHLLPQEV+VTQFNLTNGR+AIPSS
Sbjct: 661 NNRRSDLILELKRVFELLGIKYHLLPQEVVVTQFNLTNGRVAIPSS 693

BLAST of CsaV3_2G010960 vs. TAIR10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10)

HSP 1 Score: 511.1 bits (1315), Expect = 1.1e-144
Identity = 297/586 (50.68%), Postives = 410/586 (69.97%), Query Frame = 0

Query: 120 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 179
           I+   LIE   F+ I + L+ +LT+  L+    W LEVWKWC++VMV+F G LV+ W + 
Sbjct: 164 ISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMR 223

Query: 180 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMF-PDVHHN---NKVLLK 239
           ++VF+IE NF+LR +VLYFV+GL+KS Q   WL L+L+AW+++F  DV  +    KVL  
Sbjct: 224 LIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKC 283

Query: 240 VFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEE 299
           + R LI++L GA  WL+K LL+K+LA++F+V  FFDR+++SVF+ Y+L+TLSG PL EE 
Sbjct: 284 ITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEA 343

Query: 300 RDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAW 359
             + V R     H+S +            T+ +       + IDM ++ K+  E + SAW
Sbjct: 344 --ERVGREPSTGHLSFA------------TVVKKGTVKEKKVIDMGKVHKMKRE-KVSAW 403

Query: 360 SVKRLVSYVRSSGLSTISRTVDDFA------NAESEITSESEARNCAQRVFKNVAKPGAR 419
           +++ L+  VR+SGLSTIS T+D+ A       A+ EITSE EA   A  VF+NVA+P   
Sbjct: 404 TMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRNVAQPFFN 463

Query: 420 YIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKT 479
           YIEEEDLLRF+  EEV+ +FPLF+GA ETG+I++ AF  WVV  Y  R+ALAHSLNDTKT
Sbjct: 464 YIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKT 523

Query: 480 AVQQLHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIF 539
           AV+QL+KL +   XXXXXXXXXXXXXX         ++QL+ + F+  +TCK +FESI+F
Sbjct: 524 AVKQLNKLVTAILXXXXXXXXXXXXXXXXXXXXXFFSTQLVALAFIIGSTCKNLFESIVF 583

Query: 540 VFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPD 599
           VFVMHP+DVGDRCV+DGV M VEEMN+L+TVFL+ +NEK+YYPN+VL TKPISN+ RSP+
Sbjct: 584 VFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPN 643

Query: 600 MSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQ 659
           M +TV+F+I  ST    I  L++ +  Y+E  P+HW+P HS+VVKEIEN++K+KM+L   
Sbjct: 644 MGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSD 703

Query: 660 HTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFN 696
           HT+  Q   ERN RR++L L +KR+ E+L I Y LLPQ++ +T+ N
Sbjct: 704 HTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINLTKKN 734

BLAST of CsaV3_2G010960 vs. TAIR10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5)

HSP 1 Score: 490.0 bits (1260), Expect = 2.5e-138
Identity = 271/589 (46.01%), Postives = 396/589 (67.23%), Query Frame = 0

Query: 124 VLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVF 183
           V +EWI  + I T L+C+LT+ +LQ K  W L++WKW + V+V+ CGRLVS W+V ++VF
Sbjct: 269 VFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVF 328

Query: 184 VIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFP---DVHHNNKVLLKVFRFL 243
           ++E+NF  R+RVLYFVYG+RKS QNC WLGLVL+AW  +F    +    +  L  V R L
Sbjct: 329 LVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVL 388

Query: 244 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 303
           + +L+   IWL+K +LVKVLASSFH++T+FDR++ES+F  Y++ETLSGPPL E +R +E 
Sbjct: 389 VCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEE 448

Query: 304 NRR-------------RRLVHMSKSLPARWREGGGGQTLSR--SKRQDSCQKIDMERLRK 363
            ++              +L    K+    + + G    L+R  SKR +  + I +++L++
Sbjct: 449 EQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKR 508

Query: 364 LSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFANAESEIT---SESEARNCAQRVFKN 423
           ++  +  SAW++KRL++ +    +ST+ + + D    + + T   SE EA+  A+++F N
Sbjct: 509 MN-TKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFHN 568

Query: 424 VAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAH 483
           V +PG+RYI  ED LRFL +EE      LFEGA E+ KISKS  +NWVV A+ ER+ALA 
Sbjct: 569 VTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALAL 628

Query: 484 SLNDTKTAVQQLHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKT 543
           +LNDTKTAV +LH++ +      XXXX       ATT+ L V++SQLLLV F+F N+CKT
Sbjct: 629 TLNDTKTAVDRLHRIINVVIGIIXXXXWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKT 688

Query: 544 IFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPIS 603
           IFE+IIF+FVMHPFDVGDRC IDGV + VEEMNIL+TVFLR+DN+KI YPNSVL TKPI+
Sbjct: 689 IFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIA 748

Query: 604 NFRRSPDMSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKM 663
           N+ RSPDM D V+F + ++T  + ITA+++ +  Y+++K  +W P   +V   +++++ +
Sbjct: 749 NYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSV 808

Query: 664 KMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           K+++ + H MN Q+  ER  RR  L+ E+ +    L I+Y L P  + V
Sbjct: 809 KIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINV 856

BLAST of CsaV3_2G010960 vs. TAIR10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4)

HSP 1 Score: 486.5 bits (1251), Expect = 2.8e-137
Identity = 273/600 (45.50%), Postives = 388/600 (64.67%), Query Frame = 0

Query: 124 VLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVF 183
           V+IEWI  + I   LIC+L +  L+ K +W L +WKW ++V+V+ CGRLVS W+V + V+
Sbjct: 259 VIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVY 318

Query: 184 VIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFP---DVHHNNKVLLKVFRFL 243
            +E NF+ R++VLYFVYG+RK  QNC WLGLVLIAW  +F    +    + VL  V + L
Sbjct: 319 FVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVL 378

Query: 244 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPP-----LDEEE 303
           I +L+   IWL+K LLVKVLASSFH++T+FDR++ES+F  Y++ETLSGPP     ++EE+
Sbjct: 379 ICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEK 438

Query: 304 RDKEV-------NRRRRLVHMSKSLPARWREGGG-----------GQTLSR--SKRQDSC 363
              +V        +   L   + S P +   G G              LSR  SK++   
Sbjct: 439 VANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGE 498

Query: 364 QKIDMERLRKLSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFANAE----SEITSESE 423
           + I ++ L++++  +  SAW +K+L++ ++   LST+   + D    E    ++I SE E
Sbjct: 499 EGIRIDHLQRMN-TKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFE 558

Query: 424 ARNCAQRVFKNVAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVV 483
           A+  A+++F+NVA+PG+RYI  ED +RFL ++E      LFEGA E  KISKS  +NWVV
Sbjct: 559 AKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVV 618

Query: 484 HAYIERKALAHSLNDTKTAVQQLHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLL 543
           +A+ ER+ALA +LNDTKTAV +LH++                   ATTK L VI+SQLLL
Sbjct: 619 NAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLL 678

Query: 544 VGFMFQNTCKTIFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYY 603
           V F+F N+CKTIFE++IFVFVMHPFDVGDRC IDGV M VEEMNIL+TVFLRFDN+KI Y
Sbjct: 679 VVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVY 738

Query: 604 PNSVLLTKPISNFRRSPDMSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSL 663
           PNS+L TKPI+N+ RSPDM D ++F + ++T  +  TALR+ +  Y+++K  HW P   +
Sbjct: 739 PNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMI 798

Query: 664 VVKEIENVDKMKMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           V +++  ++ +K+++   H MN QN  ER  RR  L+ E+ R+   L I+Y L P  + V
Sbjct: 799 VFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINV 857

BLAST of CsaV3_2G010960 vs. TAIR10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6)

HSP 1 Score: 481.5 bits (1238), Expect = 9.0e-136
Identity = 259/589 (43.97%), Postives = 386/589 (65.53%), Query Frame = 0

Query: 124 VLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVF 183
           +++EW+  + I    +C L + SL++K++W L++WKW  +V+V+ CGRLVS W+V ++VF
Sbjct: 242 IVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVF 301

Query: 184 VIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPD---VHHNNKVLLKVFRFL 243
            IERNF+LR+RVLYFVYG+RK+ QNC WLGLVL+AW  +F +      N K L  V +  
Sbjct: 302 FIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIF 361

Query: 244 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 303
           + +L+G  +WL+K LLVKVLASSFH++T+FDR++ES+F  Y++ETLSGPPL E ++++E 
Sbjct: 362 VCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEE 421

Query: 304 NRRRRLVHMSKSLPARWREGGGGQ--------------TLSRSKRQDSCQK-IDMERLRK 363
             R  +       P       G Q               LS         K I ++ L K
Sbjct: 422 EERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLSNGXXXXXENKGITIDSLHK 481

Query: 364 LSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFA---NAESEITSESEARNCAQRVFKN 423
           L+  +  SAW +KRL++ +R+  L+T+   + D +   +  ++I SE EA+  A+++F N
Sbjct: 482 LN-PKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHN 541

Query: 424 VAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAH 483
           VAKPG+++I   D++RFL D+E      LFEGA ET +ISKS+ +NWVV+A+ ER+ALA 
Sbjct: 542 VAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALAL 601

Query: 484 SLNDTKTAVQQLHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKT 543
           +LNDTKTAV +LHK+ +                  +TK L V++SQ+++V F+F N CK 
Sbjct: 602 TLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKI 661

Query: 544 IFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPIS 603
           +FESII++FV+HPFDVGDRC IDGV M VEEMNIL+TVFLRFDN+K+ YPNS+L TK I 
Sbjct: 662 VFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIG 721

Query: 604 NFRRSPDMSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKM 663
           N+ RSPDM D ++F+I ++T  + I  +++ +  YIE K  HW P   +V K++E+++ +
Sbjct: 722 NYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSV 781

Query: 664 KMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           ++++   H MN Q+  E+  RRS L+ E+ ++   L I+Y L P ++ V
Sbjct: 782 RIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINV 829

BLAST of CsaV3_2G010960 vs. TAIR10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9)

HSP 1 Score: 468.0 bits (1203), Expect = 1.0e-131
Identity = 264/575 (45.91%), Postives = 385/575 (66.96%), Query Frame = 0

Query: 126 IEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVFVI 185
           +E ++F+ I   LI +LT++ + +  IW LE WKWC++VMV   G LV+ W +  +VF+I
Sbjct: 182 LELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVVFII 241

Query: 186 ERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFP-DVHHNNKV---LLKVFRFLI 245
           E+N++LR++VLYFV+GL+K+ Q   W  LVLIAW+ +F  DV    K    L  +   ++
Sbjct: 242 EKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRFLDFITWTIV 301

Query: 246 AVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEVN 305
           ++L+G+ ++L+K   +KVLAS F+V  FF+R++ESVF+ Y+L+TLSGPPL EE   + V 
Sbjct: 302 SLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEA--ENVG 361

Query: 306 RRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRLV 365
           R     H+S +   R ++G         K +D  + IDM ++ ++  E + SAW+++ L+
Sbjct: 362 RVPSTGHLSFT---RTKDG---------KVKDK-KVIDMGKVHRMKQE-KVSAWTMRVLI 421

Query: 366 SYVRSSGLSTISRTVDDFAN----AESEITSESEARNCAQRVFKNVAKPGARYIEEEDLL 425
             V +SG+STIS T+D+  N     + EIT+E EA   A  VF NVAKP   YIEE+DLL
Sbjct: 422 EAVGTSGISTISSTLDEVNNKKERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLL 481

Query: 426 RFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKL 485
           RF+  EEV+ + PL E A +TGKI++  F  WVV+ Y  RK + HSLNDTKTAV+QL KL
Sbjct: 482 RFMIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKL 541

Query: 486 ASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFD 545
            +                 A+TK+L V +SQ L + FM  +TCK IFES +FVFVMHP+D
Sbjct: 542 ITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYD 601

Query: 546 VGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFT 605
           VGDRCV+DGV + VEE+++L+TVFL+ DNEK++YPNSVL++KPISNF RSPDM D VDF 
Sbjct: 602 VGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFG 661

Query: 606 IDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNF 665
           I  ST  + I  L+  +  Y+ +  +HW P+  ++V+ IEN++K+ +++ VQHT+N Q +
Sbjct: 662 IAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVY 721

Query: 666 PERNNRRSDLILELKRVFENLGIKYHLLPQEVLVT 693
            E++ RR+ LI+ +KR+ E+L I Y LLPQ+V +T
Sbjct: 722 VEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNLT 739

BLAST of CsaV3_2G010960 vs. Swiss-Prot
Match: sp|Q9LYG9|MSL10_ARATH (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.9e-143
Identity = 297/586 (50.68%), Postives = 410/586 (69.97%), Query Frame = 0

Query: 120 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 179
           I+   LIE   F+ I + L+ +LT+  L+    W LEVWKWC++VMV+F G LV+ W + 
Sbjct: 164 ISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMR 223

Query: 180 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMF-PDVHHN---NKVLLK 239
           ++VF+IE NF+LR +VLYFV+GL+KS Q   WL L+L+AW+++F  DV  +    KVL  
Sbjct: 224 LIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKC 283

Query: 240 VFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEE 299
           + R LI++L GA  WL+K LL+K+LA++F+V  FFDR+++SVF+ Y+L+TLSG PL EE 
Sbjct: 284 ITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEA 343

Query: 300 RDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAW 359
             + V R     H+S +            T+ +       + IDM ++ K+  E + SAW
Sbjct: 344 --ERVGREPSTGHLSFA------------TVVKKGTVKEKKVIDMGKVHKMKRE-KVSAW 403

Query: 360 SVKRLVSYVRSSGLSTISRTVDDFA------NAESEITSESEARNCAQRVFKNVAKPGAR 419
           +++ L+  VR+SGLSTIS T+D+ A       A+ EITSE EA   A  VF+NVA+P   
Sbjct: 404 TMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRNVAQPFFN 463

Query: 420 YIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKT 479
           YIEEEDLLRF+  EEV+ +FPLF+GA ETG+I++ AF  WVV  Y  R+ALAHSLNDTKT
Sbjct: 464 YIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKT 523

Query: 480 AVQQLHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIF 539
           AV+QL+KL +   XXXXXXXXXXXXXX         ++QL+ + F+  +TCK +FESI+F
Sbjct: 524 AVKQLNKLVTAILXXXXXXXXXXXXXXXXXXXXXFFSTQLVALAFIIGSTCKNLFESIVF 583

Query: 540 VFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPD 599
           VFVMHP+DVGDRCV+DGV M VEEMN+L+TVFL+ +NEK+YYPN+VL TKPISN+ RSP+
Sbjct: 584 VFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPN 643

Query: 600 MSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQ 659
           M +TV+F+I  ST    I  L++ +  Y+E  P+HW+P HS+VVKEIEN++K+KM+L   
Sbjct: 644 MGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSD 703

Query: 660 HTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFN 696
           HT+  Q   ERN RR++L L +KR+ E+L I Y LLPQ++ +T+ N
Sbjct: 704 HTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINLTKKN 734

BLAST of CsaV3_2G010960 vs. Swiss-Prot
Match: sp|Q9LH74|MSL5_ARATH (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 4.6e-137
Identity = 271/589 (46.01%), Postives = 396/589 (67.23%), Query Frame = 0

Query: 124 VLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVF 183
           V +EWI  + I T L+C+LT+ +LQ K  W L++WKW + V+V+ CGRLVS W+V ++VF
Sbjct: 269 VFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVF 328

Query: 184 VIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFP---DVHHNNKVLLKVFRFL 243
           ++E+NF  R+RVLYFVYG+RKS QNC WLGLVL+AW  +F    +    +  L  V R L
Sbjct: 329 LVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVL 388

Query: 244 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 303
           + +L+   IWL+K +LVKVLASSFH++T+FDR++ES+F  Y++ETLSGPPL E +R +E 
Sbjct: 389 VCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEE 448

Query: 304 NRR-------------RRLVHMSKSLPARWREGGGGQTLSR--SKRQDSCQKIDMERLRK 363
            ++              +L    K+    + + G    L+R  SKR +  + I +++L++
Sbjct: 449 EQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKR 508

Query: 364 LSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFANAESEIT---SESEARNCAQRVFKN 423
           ++  +  SAW++KRL++ +    +ST+ + + D    + + T   SE EA+  A+++F N
Sbjct: 509 MN-TKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFHN 568

Query: 424 VAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAH 483
           V +PG+RYI  ED LRFL +EE      LFEGA E+ KISKS  +NWVV A+ ER+ALA 
Sbjct: 569 VTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALAL 628

Query: 484 SLNDTKTAVQQLHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKT 543
           +LNDTKTAV +LH++ +      XXXX       ATT+ L V++SQLLLV F+F N+CKT
Sbjct: 629 TLNDTKTAVDRLHRIINVVIGIIXXXXWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKT 688

Query: 544 IFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPIS 603
           IFE+IIF+FVMHPFDVGDRC IDGV + VEEMNIL+TVFLR+DN+KI YPNSVL TKPI+
Sbjct: 689 IFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIA 748

Query: 604 NFRRSPDMSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKM 663
           N+ RSPDM D V+F + ++T  + ITA+++ +  Y+++K  +W P   +V   +++++ +
Sbjct: 749 NYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSV 808

Query: 664 KMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           K+++ + H MN Q+  ER  RR  L+ E+ +    L I+Y L P  + V
Sbjct: 809 KIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINV 856

BLAST of CsaV3_2G010960 vs. Swiss-Prot
Match: sp|Q9LPG3|MSL4_ARATH (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 5.0e-136
Identity = 273/600 (45.50%), Postives = 388/600 (64.67%), Query Frame = 0

Query: 124 VLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVF 183
           V+IEWI  + I   LIC+L +  L+ K +W L +WKW ++V+V+ CGRLVS W+V + V+
Sbjct: 259 VIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVY 318

Query: 184 VIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFP---DVHHNNKVLLKVFRFL 243
            +E NF+ R++VLYFVYG+RK  QNC WLGLVLIAW  +F    +    + VL  V + L
Sbjct: 319 FVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVL 378

Query: 244 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPP-----LDEEE 303
           I +L+   IWL+K LLVKVLASSFH++T+FDR++ES+F  Y++ETLSGPP     ++EE+
Sbjct: 379 ICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEK 438

Query: 304 RDKEV-------NRRRRLVHMSKSLPARWREGGG-----------GQTLSR--SKRQDSC 363
              +V        +   L   + S P +   G G              LSR  SK++   
Sbjct: 439 VANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGE 498

Query: 364 QKIDMERLRKLSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFANAE----SEITSESE 423
           + I ++ L++++  +  SAW +K+L++ ++   LST+   + D    E    ++I SE E
Sbjct: 499 EGIRIDHLQRMN-TKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFE 558

Query: 424 ARNCAQRVFKNVAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVV 483
           A+  A+++F+NVA+PG+RYI  ED +RFL ++E      LFEGA E  KISKS  +NWVV
Sbjct: 559 AKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVV 618

Query: 484 HAYIERKALAHSLNDTKTAVQQLHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLL 543
           +A+ ER+ALA +LNDTKTAV +LH++                   ATTK L VI+SQLLL
Sbjct: 619 NAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLL 678

Query: 544 VGFMFQNTCKTIFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYY 603
           V F+F N+CKTIFE++IFVFVMHPFDVGDRC IDGV M VEEMNIL+TVFLRFDN+KI Y
Sbjct: 679 VVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVY 738

Query: 604 PNSVLLTKPISNFRRSPDMSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSL 663
           PNS+L TKPI+N+ RSPDM D ++F + ++T  +  TALR+ +  Y+++K  HW P   +
Sbjct: 739 PNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMI 798

Query: 664 VVKEIENVDKMKMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           V +++  ++ +K+++   H MN QN  ER  RR  L+ E+ R+   L I+Y L P  + V
Sbjct: 799 VFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINV 857

BLAST of CsaV3_2G010960 vs. Swiss-Prot
Match: sp|Q9SYM1|MSL6_ARATH (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 1.6e-134
Identity = 259/589 (43.97%), Postives = 386/589 (65.53%), Query Frame = 0

Query: 124 VLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVF 183
           +++EW+  + I    +C L + SL++K++W L++WKW  +V+V+ CGRLVS W+V ++VF
Sbjct: 242 IVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVF 301

Query: 184 VIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPD---VHHNNKVLLKVFRFL 243
            IERNF+LR+RVLYFVYG+RK+ QNC WLGLVL+AW  +F +      N K L  V +  
Sbjct: 302 FIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIF 361

Query: 244 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 303
           + +L+G  +WL+K LLVKVLASSFH++T+FDR++ES+F  Y++ETLSGPPL E ++++E 
Sbjct: 362 VCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEE 421

Query: 304 NRRRRLVHMSKSLPARWREGGGGQ--------------TLSRSKRQDSCQK-IDMERLRK 363
             R  +       P       G Q               LS         K I ++ L K
Sbjct: 422 EERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLSNGXXXXXENKGITIDSLHK 481

Query: 364 LSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFA---NAESEITSESEARNCAQRVFKN 423
           L+  +  SAW +KRL++ +R+  L+T+   + D +   +  ++I SE EA+  A+++F N
Sbjct: 482 LN-PKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHN 541

Query: 424 VAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAH 483
           VAKPG+++I   D++RFL D+E      LFEGA ET +ISKS+ +NWVV+A+ ER+ALA 
Sbjct: 542 VAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALAL 601

Query: 484 SLNDTKTAVQQLHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKT 543
           +LNDTKTAV +LHK+ +                  +TK L V++SQ+++V F+F N CK 
Sbjct: 602 TLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKI 661

Query: 544 IFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPIS 603
           +FESII++FV+HPFDVGDRC IDGV M VEEMNIL+TVFLRFDN+K+ YPNS+L TK I 
Sbjct: 662 VFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIG 721

Query: 604 NFRRSPDMSDTVDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKM 663
           N+ RSPDM D ++F+I ++T  + I  +++ +  YIE K  HW P   +V K++E+++ +
Sbjct: 722 NYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSV 781

Query: 664 KMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           ++++   H MN Q+  E+  RRS L+ E+ ++   L I+Y L P ++ V
Sbjct: 782 RIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINV 829

BLAST of CsaV3_2G010960 vs. Swiss-Prot
Match: sp|Q84M97|MSL9_ARATH (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.9e-130
Identity = 264/575 (45.91%), Postives = 385/575 (66.96%), Query Frame = 0

Query: 126 IEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVFVI 185
           +E ++F+ I   LI +LT++ + +  IW LE WKWC++VMV   G LV+ W +  +VF+I
Sbjct: 182 LELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVVFII 241

Query: 186 ERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFP-DVHHNNKV---LLKVFRFLI 245
           E+N++LR++VLYFV+GL+K+ Q   W  LVLIAW+ +F  DV    K    L  +   ++
Sbjct: 242 EKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRFLDFITWTIV 301

Query: 246 AVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEVN 305
           ++L+G+ ++L+K   +KVLAS F+V  FF+R++ESVF+ Y+L+TLSGPPL EE   + V 
Sbjct: 302 SLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEA--ENVG 361

Query: 306 RRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRLV 365
           R     H+S +   R ++G         K +D  + IDM ++ ++  E + SAW+++ L+
Sbjct: 362 RVPSTGHLSFT---RTKDG---------KVKDK-KVIDMGKVHRMKQE-KVSAWTMRVLI 421

Query: 366 SYVRSSGLSTISRTVDDFAN----AESEITSESEARNCAQRVFKNVAKPGARYIEEEDLL 425
             V +SG+STIS T+D+  N     + EIT+E EA   A  VF NVAKP   YIEE+DLL
Sbjct: 422 EAVGTSGISTISSTLDEVNNKKERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLL 481

Query: 426 RFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKL 485
           RF+  EEV+ + PL E A +TGKI++  F  WVV+ Y  RK + HSLNDTKTAV+QL KL
Sbjct: 482 RFMIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKL 541

Query: 486 ASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFD 545
            +                 A+TK+L V +SQ L + FM  +TCK IFES +FVFVMHP+D
Sbjct: 542 ITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYD 601

Query: 546 VGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFT 605
           VGDRCV+DGV + VEE+++L+TVFL+ DNEK++YPNSVL++KPISNF RSPDM D VDF 
Sbjct: 602 VGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFG 661

Query: 606 IDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNF 665
           I  ST  + I  L+  +  Y+ +  +HW P+  ++V+ IEN++K+ +++ VQHT+N Q +
Sbjct: 662 IAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVY 721

Query: 666 PERNNRRSDLILELKRVFENLGIKYHLLPQEVLVT 693
            E++ RR+ LI+ +KR+ E+L I Y LLPQ+V +T
Sbjct: 722 VEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNLT 739

BLAST of CsaV3_2G010960 vs. TrEMBL
Match: tr|A0A0A0LHK4|A0A0A0LHK4_CUCSA (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_2G118330 PE=3 SV=1)

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 706/706 (100.00%), Postives = 706/706 (100.00%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60
           MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP
Sbjct: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60

Query: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120
           KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI
Sbjct: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL
Sbjct: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV
Sbjct: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL
Sbjct: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL
Sbjct: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480
           KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX
Sbjct: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480

Query: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER
Sbjct: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS
Sbjct: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 706

BLAST of CsaV3_2G010960 vs. TrEMBL
Match: tr|A0A1S3C4Z6|A0A1S3C4Z6_CUCME (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103496913 PE=3 SV=1)

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 680/706 (96.32%), Postives = 686/706 (97.17%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNYPLSTP 60
           M+ RKSDNDHLVLTIDPP KEQI  XXXXXXXXXXXX          SRFDEPNYPLSTP
Sbjct: 1   MEPRKSDNDHLVLTIDPPQKEQISPXXXXXXXXXXXXLRRLNFSKPKSRFDEPNYPLSTP 60

Query: 61  KTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120
           +TIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI
Sbjct: 61  RTIPESTDLLQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTLES+QEKQIWSLEVWKWCLIVMV+FCGRLVSEWLVGV
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLESIQEKQIWSLEVWKWCLIVMVIFCGRLVSEWLVGV 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVFVIERNFMLRERVLYFVYGLRKSFQNCAWL LVLIAWMIMFPDVHH NKVLLKVFRFL
Sbjct: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLALVLIAWMIMFPDVHHKNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV
Sbjct: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NR R L+HMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL
Sbjct: 301 NRGRGLMHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL
Sbjct: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480
           K EEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX
Sbjct: 421 KVEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 480

Query: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFD ITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER
Sbjct: 601 STSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFENLGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 706

BLAST of CsaV3_2G010960 vs. TrEMBL
Match: tr|A0A2N9IP40|A0A2N9IP40_FAGSY (Mechanosensitive ion channel protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS53946 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 4.0e-255
Identity = 513/712 (72.05%), Postives = 582/712 (81.74%), Query Frame = 0

Query: 2   DVRKSDNDHLVLTIDPPD------KEQIXXXXXXXXXXXXXXXXXXXXXXXXSRFDEPNY 61
           D +K++ DH+VL I+ PD       +                          SRF+E NY
Sbjct: 6   DHQKNNTDHVVLNIENPDPKLKQSPQADTNEPLSQTQTRTRTLKRLNFSKPRSRFEEINY 65

Query: 62  PLSTPKTIPESTDL--LQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 121
           PL  P+TI ES +L  L P             XXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 66  PL-PPRTILESEELEYLNP--------HDDTSXXXXXXXXXXXXXXXXXXXXXXXXXXXX 125

Query: 122 XXXXXXINKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLV 181
           XXXXXXINKR +IEW LFLTI TCL+C+LT +SL+ ++ W LE+WKWCL+VMVVFCGRLV
Sbjct: 126 XXXXXXINKRAVIEWTLFLTIMTCLVCSLTQKSLKYEKKWGLEIWKWCLMVMVVFCGRLV 185

Query: 182 SEWLVGVLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVL 241
           S W+VG LVF+IERNFMLRE+VLYFVYGLRKSFQNC WLGLVL+AWMIMFP+VH  NK L
Sbjct: 186 SGWVVGFLVFLIERNFMLREKVLYFVYGLRKSFQNCFWLGLVLVAWMIMFPNVHEKNKFL 245

Query: 242 LKVFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDE 301
           +KVFR LIAVL+ ATIWLLKI+ VKVLASSFHVATFFDRMKESVFNHYILETLSGP LDE
Sbjct: 246 MKVFRALIAVLVAATIWLLKIVFVKVLASSFHVATFFDRMKESVFNHYILETLSGPQLDE 305

Query: 302 EERDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPS 361
           EERD      RR +  SK++PAR R+      L+RSKR  S ++IDMERLRKLS E RP+
Sbjct: 306 EERDLP----RRRLQASKTMPARVRD--KSYALTRSKRYGS-RRIDMERLRKLSSEARPT 365

Query: 362 AWSVKRLVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEE 421
           AWSVKRLVSYVRSSGLSTISRTVDDF  AESEI SE EARNCAQR+FKNVAKP A+YI+E
Sbjct: 366 AWSVKRLVSYVRSSGLSTISRTVDDFGKAESEINSEWEARNCAQRIFKNVAKPNAKYIDE 425

Query: 422 EDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQ 481
           +DLL+FLK EEV+TIFPLFEGA+ETG+I+KS+FRNWVVHAYIERKALAHSLNDTKTAVQQ
Sbjct: 426 DDLLKFLKSEEVHTIFPLFEGAVETGRITKSSFRNWVVHAYIERKALAHSLNDTKTAVQQ 485

Query: 482 LHKLASXXXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVM 541
           LHKLAS                 ATTKV+FV+TSQLLLVGFMFQN CKT+FESIIFVFVM
Sbjct: 486 LHKLASAIVIVIISVVSLLVMGLATTKVIFVVTSQLLLVGFMFQNMCKTVFESIIFVFVM 545

Query: 542 HPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDT 601
           HPFDVGDRCV+DGV M VEEMNILSTVFLR+DNEKIYYPNSVLLTKPISNFRRSPDM+D 
Sbjct: 546 HPFDVGDRCVVDGVQMIVEEMNILSTVFLRYDNEKIYYPNSVLLTKPISNFRRSPDMADC 605

Query: 602 VDFTIDVSTSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMN 661
           +DF+IDVST  D ITAL+KA+Q+Y+ESKPKHWSPKHS++VKEIEN++K+KM+LCVQHTMN
Sbjct: 606 IDFSIDVSTPIDDITALKKAIQVYLESKPKHWSPKHSVIVKEIENLNKLKMALCVQHTMN 665

Query: 662 LQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPS 706
            QN+ ERN+RRS+L+LELKR+FE+LGIKYH LPQEV VTQFN+TNGRMAIPS
Sbjct: 666 HQNYGERNSRRSELVLELKRIFESLGIKYHFLPQEVHVTQFNVTNGRMAIPS 701

BLAST of CsaV3_2G010960 vs. TrEMBL
Match: tr|M5XJX8|M5XJX8_PRUPE (Mechanosensitive ion channel protein OS=Prunus persica OX=3760 GN=PRUPE_2G100800 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.2e-243
Identity = 435/585 (74.36%), Postives = 505/585 (86.32%), Query Frame = 0

Query: 122 KRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVL 181
           KR +IEW LFL I TCL+C+LTL+ L  K  W LE+WKWCL+VMV+FCGRLVS W+VG L
Sbjct: 120 KRAVIEWTLFLIIMTCLVCSLTLDFLINKLKWGLEIWKWCLMVMVIFCGRLVSGWVVGFL 179

Query: 182 VFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFLI 241
           VF+IERNFMLRE+VLYFV+GLR+SFQNCAWLGLVL AWMIMFPDVH ++KVL KVFR LI
Sbjct: 180 VFLIERNFMLREKVLYFVFGLRRSFQNCAWLGLVLAAWMIMFPDVHKHSKVLKKVFRALI 239

Query: 242 AVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEVN 301
           AVLIGATIWLLKIL VKVLASSFHV+TFFDRMKESVF+HYILE LSGPPLDE+ER++   
Sbjct: 240 AVLIGATIWLLKILFVKVLASSFHVSTFFDRMKESVFHHYILEALSGPPLDEDEREE--- 299

Query: 302 RRRRLVHMSKSLPARWREGGGGQTLSRSKRQD-SCQKIDMERLRKLSLERRPSAWSVKRL 361
             RR    SKSLPAR R+    Q +SRS RQ    ++IDM++LR+LS+ RR +AWSVK+L
Sbjct: 300 LPRRPFQASKSLPARLRD--KSQAMSRSNRQGYESRRIDMKKLRRLSMTRRATAWSVKKL 359

Query: 362 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 421
           V+YVRSSGLSTISRT+DDF NAESEITSE +ARN AQR+FKNVAKPGA+YIEEEDLLRFL
Sbjct: 360 VNYVRSSGLSTISRTIDDFGNAESEITSEWDARNSAQRIFKNVAKPGAKYIEEEDLLRFL 419

Query: 422 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASX 481
           + + ++T FPLFEGAIETG+I+KS+FRNWVVHAYIERKALAHSLNDTKTAV QLHKLAS 
Sbjct: 420 RRDAIHTTFPLFEGAIETGRITKSSFRNWVVHAYIERKALAHSLNDTKTAVHQLHKLASG 479

Query: 482 XXXXXXXXXXXXXXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 541
                           ATTKV+FV+TSQLLLVGFMFQN CKT+FESIIFVFVMHPFDVGD
Sbjct: 480 IVIVIISVVSLLVMGLATTKVIFVVTSQLLLVGFMFQNMCKTVFESIIFVFVMHPFDVGD 539

Query: 542 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 601
           RCV++GV M VEEMNILSTVFLR+DNEKIYYPNSVLLTKPISNFRRSPDM+DTVDFTIDV
Sbjct: 540 RCVVEGVQMIVEEMNILSTVFLRYDNEKIYYPNSVLLTKPISNFRRSPDMADTVDFTIDV 599

Query: 602 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 661
           ST  D ++AL+K++Q YIESK K+W+PKHS++VKEIENVDKMKM+LCVQHTMN QN+ E+
Sbjct: 600 STPVDDVSALKKSIQSYIESKSKYWNPKHSVIVKEIENVDKMKMTLCVQHTMNHQNYGEK 659

Query: 662 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPS 706
           + RRS+L+ ELK++F+NLGI+YHLLPQEV +TQ N +NGR+ IPS
Sbjct: 660 SARRSELVFELKKIFQNLGIEYHLLPQEVNLTQLNASNGRLTIPS 699

BLAST of CsaV3_2G010960 vs. TrEMBL
Match: tr|A0A2I4G2Z6|A0A2I4G2Z6_9ROSI (Mechanosensitive ion channel protein OS=Juglans regia OX=51240 GN=LOC109004248 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.2e-240
Identity = 447/574 (77.87%), Postives = 504/574 (87.80%), Query Frame = 0

Query: 134 ITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVFVIERNFMLRE 193
           I TCL+C+LTL S++ K+ W LE+WKWCL+VMV+FCGRLVS W+VG L F+IERNFMLRE
Sbjct: 137 IMTCLVCSLTLNSVKNKKKWGLEIWKWCLMVMVIFCGRLVSGWVVGFLAFLIERNFMLRE 196

Query: 194 RVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFLIAVLIGATIWLLK 253
           +VLYFVYGLRKSF+NC WLGLVL+AWMIMFP+VH +NK L KVFR LIAVLIGATIWLLK
Sbjct: 197 KVLYFVYGLRKSFRNCVWLGLVLVAWMIMFPNVHEHNKFLKKVFRALIAVLIGATIWLLK 256

Query: 254 ILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEVNRRRRLVHMSKSL 313
           IL VKVLASSFHVATFFDRMKESVF+HYILETLSGPPLDE+ER   V  +RR +  SK+L
Sbjct: 257 ILFVKVLASSFHVATFFDRMKESVFHHYILETLSGPPLDEDER---VLPQRRRLQASKTL 316

Query: 314 PARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRLVSYVRSSGLSTIS 373
           PAR                    KIDMERLRKLSLE RP+AWS+KRLVS+V SSGLSTIS
Sbjct: 317 PAR--------------------KIDMERLRKLSLEGRPTAWSMKRLVSHVMSSGLSTIS 376

Query: 374 RTVDDFANAESEITSESEARNCAQRVFKNV-AKPGARYIEEEDLLRFLKDEEVNTIFPLF 433
           RTVDDF  AESEITSE EARNCAQR+FKN+ AKPGA+YIEEEDLLRFL  EEVNTIFPLF
Sbjct: 377 RTVDDFGKAESEITSEREARNCAQRIFKNIAAKPGAKYIEEEDLLRFLHIEEVNTIFPLF 436

Query: 434 EGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASXXXXXXXXXXXXX 493
           EGA++T +I+KS+FRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS  XXXXXXXXXXX
Sbjct: 437 EGAVKTERITKSSFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASAVXXXXXXXXXXX 496

Query: 494 XXXXATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGDRCVIDGVHMFVE 553
           XX  ATTKV+FV+TSQLLLVGFMFQN CKT+FESIIFVFVMHPFDVGDRCV++GV M VE
Sbjct: 497 XXGLATTKVIFVVTSQLLLVGFMFQNMCKTMFESIIFVFVMHPFDVGDRCVVEGVQMIVE 556

Query: 554 EMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDVSTSFDIITALRK 613
           EMNIL+TVFLR+DNEKIYYPN+VLLTKPISNFRRSPDM+D +DF+IDVST  D ITAL+K
Sbjct: 557 EMNILTTVFLRYDNEKIYYPNAVLLTKPISNFRRSPDMADCIDFSIDVSTPADDITALKK 616

Query: 614 AMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPERNNRRSDLILELK 673
           A+Q YIESKPKHWSPKHS++VKEIENV+KMKMSLCVQHTMN QN+ E+++RRS+L+LELK
Sbjct: 617 AIQSYIESKPKHWSPKHSVLVKEIENVNKMKMSLCVQHTMNHQNYGEKSSRRSELVLELK 676

Query: 674 RVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           R+FE L I+YHLLPQE+ VTQFN+TNGRM  PSS
Sbjct: 677 RIFEILRIRYHLLPQEINVTQFNVTNGRMPFPSS 687

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011649063.10.0e+00100.00PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis sativus] >KGN61... [more]
XP_008457175.10.0e+0096.32PREDICTED: mechanosensitive ion channel protein 10 [Cucumis melo][more]
XP_022964525.11.8e-30787.96mechanosensitive ion channel protein 10-like [Cucurbita moschata][more]
XP_023513519.11.5e-30687.68mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo][more]
XP_023000433.11.4e-30487.96mechanosensitive ion channel protein 10-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G12080.11.1e-14450.68mechanosensitive channel of small conductance-like 10[more]
AT3G14810.12.5e-13846.01mechanosensitive channel of small conductance-like 5[more]
AT1G53470.12.8e-13745.50mechanosensitive channel of small conductance-like 4[more]
AT1G78610.19.0e-13643.97mechanosensitive channel of small conductance-like 6[more]
AT5G19520.11.0e-13145.91mechanosensitive channel of small conductance-like 9[more]
Match NameE-valueIdentityDescription
sp|Q9LYG9|MSL10_ARATH1.9e-14350.68Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10... [more]
sp|Q9LH74|MSL5_ARATH4.6e-13746.01Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
sp|Q9LPG3|MSL4_ARATH5.0e-13645.50Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
sp|Q9SYM1|MSL6_ARATH1.6e-13443.97Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
sp|Q84M97|MSL9_ARATH1.9e-13045.91Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 P... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LHK4|A0A0A0LHK4_CUCSA0.0e+00100.00Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_2G118330 ... [more]
tr|A0A1S3C4Z6|A0A1S3C4Z6_CUCME0.0e+0096.32Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103496913 PE=... [more]
tr|A0A2N9IP40|A0A2N9IP40_FAGSY4.0e-25572.05Mechanosensitive ion channel protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS539... [more]
tr|M5XJX8|M5XJX8_PRUPE1.2e-24374.36Mechanosensitive ion channel protein OS=Prunus persica OX=3760 GN=PRUPE_2G100800... [more]
tr|A0A2I4G2Z6|A0A2I4G2Z6_9ROSI1.2e-24077.87Mechanosensitive ion channel protein OS=Juglans regia OX=51240 GN=LOC109004248 P... [more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: Biological Process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: INTERPRO
TermDefinition
IPR010920LSM_dom_sf
IPR016688MscS-like_plants/fungi
IPR006685MscS_channel
IPR023408MscS_dom_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0007165 signal transduction
biological_process GO:0045892 negative regulation of transcription, DNA-templated
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0003674 molecular_function
molecular_function GO:0003714 transcription corepressor activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G010960.1CsaV3_2G010960.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3DG3DSA:2.30.30.60coord: 535..584
e-value: 8.6E-9
score: 37.0
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 476..681
e-value: 4.3E-26
score: 91.6
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRSFPIRSF017209Memb_At2g17000coord: 3..704
e-value: 1.4E-194
score: 646.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..37
NoneNo IPR availablePANTHERPTHR31618FAMILY NOT NAMEDcoord: 25..695
NoneNo IPR availablePANTHERPTHR31618:SF7SUBFAMILY NOT NAMEDcoord: 25..695
IPR010920LSM domain superfamilySUPERFAMILYSSF50182Sm-like ribonucleoproteinscoord: 525..584