CsaV3_2G008140 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_2G008140 vs. NCBI nr
Match: XP_011650116.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis sativus])

HSP 1 Score: 1043.5 bits (2697), Expect = 2.5e-301
Identity = 530/531 (99.81%), Postives = 531/531 (100.00%), Query Frame = 0

Query: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60
           MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 14  MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 73

Query: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120
           SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV
Sbjct: 74  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 133

Query: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180
           NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP
Sbjct: 134 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 193

Query: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240
           D+GFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG
Sbjct: 194 DSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 253

Query: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300
           VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG
Sbjct: 254 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 313

Query: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 360
           QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX
Sbjct: 314 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 373

Query: 361 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420
           XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL
Sbjct: 374 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 433

Query: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480
           KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV
Sbjct: 434 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 493

Query: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNLK 532
           DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNLK
Sbjct: 494 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNLK 544

BLAST of CsaV3_2G008140 vs. NCBI nr
Match: KGN61112.1 (hypothetical protein Csa_2G055000 [Cucumis sativus])

HSP 1 Score: 1015.4 bits (2624), Expect = 7.2e-293
Identity = 516/517 (99.81%), Postives = 517/517 (100.00%), Query Frame = 0

Query: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60
           MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 14  MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 73

Query: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120
           SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV
Sbjct: 74  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 133

Query: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180
           NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP
Sbjct: 134 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 193

Query: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240
           D+GFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG
Sbjct: 194 DSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 253

Query: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300
           VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG
Sbjct: 254 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 313

Query: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 360
           QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX
Sbjct: 314 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 373

Query: 361 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420
           XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL
Sbjct: 374 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 433

Query: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480
           KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV
Sbjct: 434 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 493

Query: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 518
           DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Sbjct: 494 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of CsaV3_2G008140 vs. NCBI nr
Match: XP_008452041.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 973.4 bits (2515), Expect = 3.1e-280
Identity = 482/530 (90.94%), Postives = 505/530 (95.28%), Query Frame = 0

Query: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60
           MISIFQLQLHSS AIPNQDV YMKFVHNATDLP KEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 14  MISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKF 73

Query: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120
           SVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRFVSEDG+ENIR RVLGGGSMV
Sbjct: 74  SVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIENIRARVLGGGSMV 133

Query: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180
           NAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGV+P
Sbjct: 134 NAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVIP 193

Query: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240
           DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EATVQRILFSGLSANG
Sbjct: 194 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEATVQRILFSGLSANG 253

Query: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300
           VLYSDSKGKLH+A IR+KGEII+SAGAIGSPQLLLLSGIGPKS+LSSLKLPVVLHQPYVG
Sbjct: 254 VLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPYVG 313

Query: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 360
           QSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASPFPIL+P IFS        
Sbjct: 314 QSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILVPPIFS-FLTQVTS 373

Query: 361 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420
              T A+FVGKFSEVHSEGSLRLNSSTNVK+SPIV FNYYSHPDDLGRCVKGVRK+GDLL
Sbjct: 374 ITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLGRCVKGVRKIGDLL 433

Query: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480
           KT+TMEKIKT+NLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TYWHYHGGC+VGKVV
Sbjct: 434 KTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTYWHYHGGCVVGKVV 493

Query: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNL 531
           DGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLK+L +R NL
Sbjct: 494 DGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKVLQERLNL 542

BLAST of CsaV3_2G008140 vs. NCBI nr
Match: XP_008451945.2 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 947.6 bits (2448), Expect = 1.8e-272
Identity = 470/530 (88.68%), Postives = 491/530 (92.64%), Query Frame = 0

Query: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60
           MISIFQLQLHSSHAIPNQDVSYMKFVHNATDL  KEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLSTKEEYDYIIIGGGTAGCPLATTLSSKF 60

Query: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120
           SVL+LERGSDPNKYP VL+E GL NVF AGDDG+NP QRF+SEDGVENIRGRVLGGGSMV
Sbjct: 61  SVLVLERGSDPNKYPFVLNELGLFNVFTAGDDGKNPSQRFISEDGVENIRGRVLGGGSMV 120

Query: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180
           NAGFYSRGHREFF SAGVDWDMELVEKAYEWVEETVVSQPILNAWQS FRSSLLE GVVP
Sbjct: 121 NAGFYSRGHREFFESAGVDWDMELVEKAYEWVEETVVSQPILNAWQSTFRSSLLESGVVP 180

Query: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240
           DNGFDLRHLVGTKTGGSIFDN GNRHGAVE LNKAN TNIKVAIEATVQRILFSGLSANG
Sbjct: 181 DNGFDLRHLVGTKTGGSIFDNIGNRHGAVEFLNKANTTNIKVAIEATVQRILFSGLSANG 240

Query: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300
           VLYSDSKGKLH+A IRKKGEII+SAGAIGSPQLLLLSGIGPKS+LSSLKLP+VLHQPYVG
Sbjct: 241 VLYSDSKGKLHKAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSYLSSLKLPLVLHQPYVG 300

Query: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 360
           QSMSDNPRFGT  I+PFPVLPSS K VGILQDNIYIQSIAS  PILIP IFS        
Sbjct: 301 QSMSDNPRFGTYFILPFPVLPSSAKAVGILQDNIYIQSIASLLPILIPPIFSFLPPGATS 360

Query: 361 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420
              +L + +GKFSEVHSEGSLRLNSST+VK+SPIV FNYYSHPDDLGRCVKGVRK+GDLL
Sbjct: 361 IDPSLVVLIGKFSEVHSEGSLRLNSSTDVKRSPIVRFNYYSHPDDLGRCVKGVRKIGDLL 420

Query: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480
           KT+TMEKIKT+NLE NKGFEFLGVPLPENL N S+VEEYCKKTV+TYWHYHGGC+VGKVV
Sbjct: 421 KTKTMEKIKTQNLEDNKGFEFLGVPLPENLGNVSAVEEYCKKTVSTYWHYHGGCVVGKVV 480

Query: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNL 531
           DGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLKLL QRSNL
Sbjct: 481 DGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKLLQQRSNL 530

BLAST of CsaV3_2G008140 vs. NCBI nr
Match: XP_008452065.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 889.8 bits (2298), Expect = 4.5e-255
Identity = 443/527 (84.06%), Postives = 475/527 (90.13%), Query Frame = 0

Query: 2   ISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFS 61
           ISIF LQL SS AIPNQDVSYMKFVHNATDLP KEEYDYIIIGGGTAGCPLATTLSSKFS
Sbjct: 15  ISIFPLQLLSSEAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFS 74

Query: 62  VLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVN 121
           +L+LERGSDPNKYPSVL+ QG+ N FAAGDDG+NPFQ FVSEDGVENIRGRVLGGGSMVN
Sbjct: 75  ILVLERGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSMVN 134

Query: 122 AGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPIL-NAWQSAFRSSLLEGGVVP 181
           AGFYSRGHREFF +AGVDWDMELVEKAYEWVEETVVSQPIL NAWQ+AFRS+LLEGGVVP
Sbjct: 135 AGFYSRGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGVVP 194

Query: 182 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 241
           DNGFDLRHLVGTK GGSIFDNKGNRHGAVELLNKANPTNIKVAI+ATVQ+ILFSGLSA G
Sbjct: 195 DNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVAIQATVQKILFSGLSATG 254

Query: 242 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 301
           V YSDSKGKLH A I K GEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQP+VG
Sbjct: 255 VSYSDSKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVG 314

Query: 302 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 361
           Q M+DNPRFGT I++PF +LPS+ KVVGILQ+NIYIQS A P   L+P  FS        
Sbjct: 315 QFMTDNPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPSTFLVPPAFSLLPPQSTS 374

Query: 362 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 421
              TLA FVGKFS+V+S+G L LNSS +VKKSPIV FNYYSH DDL RCV+GVRK+GDLL
Sbjct: 375 INPTLASFVGKFSDVNSKGWLHLNSSLDVKKSPIVRFNYYSHHDDLDRCVRGVRKVGDLL 434

Query: 422 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 481
           KT+T+EKIKT++LEG K F FLG+PL ENLWNDSSV+EYCKKTVTTYWHYHGGCLVGKVV
Sbjct: 435 KTQTIEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTVTTYWHYHGGCLVGKVV 494

Query: 482 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 528
           DGNYKVIGI+NLRVVDGSTFS SPGTNPMATLMMLGRYVGLK+L QR
Sbjct: 495 DGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLKQR 541

BLAST of CsaV3_2G008140 vs. TAIR10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 523.5 bits (1347), Expect = 1.6e-148
Identity = 260/529 (49.15%), Postives = 356/529 (67.30%), Query Frame = 0

Query: 9   LHSSHAIP--NQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLE 68
           +H S+A P  N+   +M+F+ NATD   ++ YDYII+GGGTAGCPLA TLS  F VLLLE
Sbjct: 23  VHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLE 82

Query: 69  RGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYS 128
           RG  P   P+V+   G L      ++  +P Q F+SE+GV N RGRVLGG S +NAGFYS
Sbjct: 83  RGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYS 142

Query: 129 RGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDNGFDL 188
           R  ++FF ++G+ WD+  V ++YEWVE  +V +P L  WQ+A R +LLE GV P NGF L
Sbjct: 143 RADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTL 202

Query: 189 RHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILF--------SGLSA 248
            H VGTK GGS FD  G RH + +LL  A  +NI+VA+ ATV+R+L         S +SA
Sbjct: 203 EHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSA 262

Query: 249 NGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPY 308
            GV+Y D  G+ H A+IR +GE+I+SAGA+GSPQLL LSGIGP+S+LS+  +PV L QP+
Sbjct: 263 IGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPH 322

Query: 309 VGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXX 368
           VG  + DNPR G +I+ P P+  S ++VVG+ +D  ++++ ++  P   P          
Sbjct: 323 VGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPA 382

Query: 369 XXXXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGD 428
                 +   + K     S G LRL +ST+V+ +P+V FNY+S P DL RCV G RK+G+
Sbjct: 383 SPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGE 442

Query: 429 LLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGK 488
           +L++R M+    +   GN+ F F+G PLP +  ND  + ++C++TV+T WHYHGG +VGK
Sbjct: 443 ILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGK 502

Query: 489 VVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 528
           VVD + KVIG+ +LR+VDGSTF+ SPGTNP ATLMMLGRY+GLK+L +R
Sbjct: 503 VVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

BLAST of CsaV3_2G008140 vs. TAIR10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 422.9 bits (1086), Expect = 2.9e-118
Identity = 249/573 (43.46%), Postives = 326/573 (56.89%), Query Frame = 0

Query: 7   LQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLE 66
           L LHS     ++  +Y  F+ +AT  P    YDYIIIGGGTAGCPLA TLS   SVLLLE
Sbjct: 16  LFLHSPICSSDKAPNY-SFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLE 75

Query: 67  RGSDPNKYPSVLDEQGLLNVFAAGDDG---RNPFQRFVSEDGVENIRGRVLGGGSMVNAG 126
           RG  P   P++      L+ F A        +P QRFVSEDGV N R RVLGGGS +NAG
Sbjct: 76  RGDSPYNNPNIT----RLSAFGAALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAG 135

Query: 127 FYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDNG 186
           FY+R   ++  + G  WD  L  ++Y+WVE  V  QP +  WQ+A R  LLE G+VP+NG
Sbjct: 136 FYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNG 195

Query: 187 FDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLS-----A 246
           F   H+ GTK GG+IFD  GNRH A +LL  A+P  I V + ATV RILF         A
Sbjct: 196 FTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIA 255

Query: 247 NGVLYSDSKGKLHRAIIRK--KGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQ 306
           NGV+Y D  G+ HRA +++    EII+SAG +GSPQLL+LSG+GP + L +  + VV+ Q
Sbjct: 256 NGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQ 315

Query: 307 PYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQ------------------- 366
           P+VGQ M DNP     +  P PV  S ++VVGI  +  Y++                   
Sbjct: 316 PHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAXXXXXXXXXXXXXXXXXX 375

Query: 367 -----SIASPFPILIP--QIFSXXXXXXXXXXXTLAMFVGKFSEVHSEGSLRLNSSTNVK 426
                ++ SP   L+    +              L   +G  S  H E   R     N K
Sbjct: 376 XXXXYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTR-----NPK 435

Query: 427 KSPIVGFNYYSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEF---LGVPLP 486
            +PIV FNY+ HPDDL RCV+G++ +  +++++   + K  ++     FE+   L    P
Sbjct: 436 DNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFEYLLNLTASTP 495

Query: 487 ENLWN---------DSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGS 532
            NL             S EE+C+ TVTT WHYHGGC+VG+VVDG+YKVIGI  LRV+D S
Sbjct: 496 VNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMS 555

BLAST of CsaV3_2G008140 vs. TAIR10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 408.7 bits (1049), Expect = 5.6e-114
Identity = 239/537 (44.51%), Postives = 318/537 (59.22%), Query Frame = 0

Query: 38  YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPF 97
           YDYI+IGGGTAGCPLA TLS  FSVL+LERG  P    +V   +   ++  A     +  
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN-FHIGLADISASSAS 122

Query: 98  QRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVV 157
           Q FVS DGV N R RVLGGGS +NAGFYSR    F   AG  WD +LV+++Y WVE  +V
Sbjct: 123 QAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVEREIV 182

Query: 158 SQPILNAWQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANP 217
            QP L  WQ A R SLLE GV P NGF   H+ GTK GG+IFD  G RH A ELL  ANP
Sbjct: 183 HQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANP 242

Query: 218 TNIKVAIEATVQRILF--SGL--SANGVLYSDSKGKLHRAII--RKKGEIIVSAGAIGSP 277
             ++V I ATVQ+I+F  SG      GV++ D KG  H+A++  RK  E+I+S+GAIGSP
Sbjct: 243 QKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSP 302

Query: 278 QLLLLSGIGPKSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIP--FPVLPSSVKVVGI 337
           Q+L+LSGIGPK  L  LK+PVVL   +VG+ M+DNP     I++P   P+  S ++ VGI
Sbjct: 303 QMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NTILVPSKAPIEQSLIQTVGI 362

Query: 338 LQDNIYI----------QSIASPFPILI--PQIFSXXXXXXXXXXXTLAMFVGKFSEVH- 397
            +  +Y+          +SI + + I+    ++FS           T A       ++H 
Sbjct: 363 TKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHE 422

Query: 398 ---------------SEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLLK 457
                          S G L L  +TNV  +P V FNY+ HP DL RCV+ +R +  ++ 
Sbjct: 423 AFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVT 482

Query: 458 T-----------RTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHY 517
           +           + + K+ + +++ N          P+ L +  S+ ++CK TV T WHY
Sbjct: 483 SNRFLNYTQCDKQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVTIWHY 542

Query: 518 HGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 528
           HGGCLVGKVV  N KV+G+  LRV+DGSTF ESPGTNP AT+MM+GRY+G+K+L +R
Sbjct: 543 HGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

BLAST of CsaV3_2G008140 vs. TAIR10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 403.3 bits (1035), Expect = 2.3e-112
Identity = 237/541 (43.81%), Postives = 314/541 (58.04%), Query Frame = 0

Query: 24  KFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGL 83
           +F+ +AT  P    +DYIIIGGGTAGC LA TLS   +VL+LERG  P   P+  D    
Sbjct: 32  RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAATDIGNF 91

Query: 84  LNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDME 143
            N         +  Q F+SEDGV N R RVLGGG+++NAGFYSR   +F A AG  W+ +
Sbjct: 92  ANTL-LNITPNSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERD 151

Query: 144 LVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKG 203
            VE AYEWVE+ VV +P +N WQSAFR  LLE GV P NGF   H+VGTK GG+IFD  G
Sbjct: 152 EVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDG 211

Query: 204 NRHGAVELLNKANPTNIKVAIEATVQRILFS-----GLSANGVLYSDSKGKLHRAIIRKK 263
           +RH A  LL  ANP  I V + A+V +ILF+        A GV++ D+ G  ++A +  +
Sbjct: 212 HRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAELATQ 271

Query: 264 ----GEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKL-PVVLHQPYVGQSMSDNPRFGTNI 323
                E+I+SAGAI SPQLL+LSG+GP +HL++ ++ PV++ QP VGQ M DNP     I
Sbjct: 272 DSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFI 331

Query: 324 IIPFPVLPSSVKVVGILQDNIYIQ---------SIASPF-------------PILIPQIF 383
             P PV  S V+ VGI +   YI+         S+   F             P      F
Sbjct: 332 PSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSISKF 391

Query: 384 SXXXXXXXXXXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVK 443
                          + + K +   S G L L  +TN   +P V FNY+  P+DL +CV+
Sbjct: 392 FKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELR-NTNPDDNPSVTFNYFKDPEDLNKCVE 451

Query: 444 GVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENL-----WNDSSVEEYCKKTVTT 503
           G+  +  ++ ++   K K   L   +G   L + LP NL      +   +E+YC  TV T
Sbjct: 452 GLSTIIKVIDSKGYSKYKYP-LASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMT 511

Query: 504 YWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQ 528
            +HYHGGC VGKVVD NYKV+G+  LR++DGSTF +SPGTNP AT+MMLGRY+G K+L +
Sbjct: 512 IYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLKSPGTNPQATIMMLGRYMGQKILRE 567

BLAST of CsaV3_2G008140 vs. TAIR10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 394.8 bits (1013), Expect = 8.3e-110
Identity = 227/527 (43.07%), Postives = 310/527 (58.82%), Query Frame = 0

Query: 25  FVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLL 84
           F+ +AT  P    +DYIIIGGGTAGC LA TLS   SVL+LERG  P + P+  D    +
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 85  NVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMEL 144
           N     +   +  Q F+SEDGV N R RVLGGGS++N GFYSR   ++   A  +W+ME 
Sbjct: 120 NTL-LNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEE 179

Query: 145 VEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGN 204
           VE AYEWVE+ +V +P +  WQ AF+  LLE G  PDNGF   H+ GTK GG+IFD  G+
Sbjct: 180 VEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGH 239

Query: 205 RHGAVELLNKANPTNIKVAIEATVQRILFSGLSANGVLYSDSKGKLHRAIIRKK--GEII 264
           RH A  LL  ANP  I V + A+V ++LF+   A  VL+ D+ G  H+A +  K   E+I
Sbjct: 240 RHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATNEVI 299

Query: 265 VSAGAIGSPQLLLLSGIGPKSHLSSLKL-PVVLHQPYVGQSMSDNPRFGTNIIIPFPVLP 324
           +SAGA+GSPQLL+LSG+GP  HL +  + P+VL QP VGQ M+DNP     I  P PV  
Sbjct: 300 LSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVEL 359

Query: 325 SSVKVVGILQDNIYIQSIAS---PFPIL------IPQIFSXXXXXXXXXXXT--LAMFVG 384
           S ++ VGI + + YI+ ++     F I       +  + +           T  +A+ + 
Sbjct: 360 SLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLLK 419

Query: 385 KFS---EVHSEGSLRLNS-------------STNVKKSPIVGFNYYSHPDDLGRCVKGVR 444
            F    EV   G L                 +TN + +P V FNYY  P+DL +CVKG+ 
Sbjct: 420 SFDVKLEVRMNGGLIFQKVDGPASKGHMKLRNTNPRDNPSVTFNYYQEPEDLNKCVKGLN 479

Query: 445 KMGDLLKTRTMEKIKTKNLEGNKGFE-FLGVPL---PENLWNDSSVEEYCKKTVTTYWHY 504
            +  ++ ++   K K   +   +     L +P+   P ++ +  +++++C  TVT+ WHY
Sbjct: 480 TIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVWHY 539

Query: 505 HGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 518
           HGGC VGKVVD NYKV+GI  LRV+DGSTF +SPGTNP AT+MMLGR
Sbjct: 540 HGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582

BLAST of CsaV3_2G008140 vs. Swiss-Prot
Match: sp|P52706|MDL1_PRUSE ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 5.4e-159
Identity = 272/524 (51.91%), Postives = 364/524 (69.47%), Query Frame = 0

Query: 8   QLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLER 67
           ++HS     N D SY++F ++ATDL  +  YDY+I+GGGT+GCPLA TLS K+ VL+LER
Sbjct: 24  EVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLER 83

Query: 68  GSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSR 127
           GS P  YP+VL   G +      DDG+ P +RFVSEDG++N+RGRVLGG SM+NAG Y+R
Sbjct: 84  GSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYAR 143

Query: 128 GHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDNGFDLR 187
            +   ++++GVDWDM+LV K YEWVE+T+V +P    WQS   ++ LE GV P++GF L 
Sbjct: 144 ANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLD 203

Query: 188 HLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFS---GLSANGVLYS 247
           H  GT+  GS FDNKG RH A ELLNK N  N++V + A+V++I+FS   GL+A GV+Y 
Sbjct: 204 HEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYR 263

Query: 248 DSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGQSMS 307
           DS G  HRA +R KGE+IVSAG IG+PQLLLLSG+GP+S+LSSL +PVVL  PYVGQ + 
Sbjct: 264 DSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLH 323

Query: 308 DNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXXXXXT 367
           DNPR   NI+ P P+ P+ V V+GI  D  + Q   S  P   P               T
Sbjct: 324 DNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPPFSFFPSTSYPLPNST 383

Query: 368 LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLLKTRT 427
            A F  K +   S GSL L SS+NV+ SP V FNYYS+P DL  CV G++K+G+LL T  
Sbjct: 384 FAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDA 443

Query: 428 MEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNY 487
           ++  K ++L G +GF  LG+PLP++  +D++ E +C+++V +YWHYHGGCLVGKV+DG++
Sbjct: 444 LKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDF 503

Query: 488 KVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRS 529
           +V GI  LRVVDGSTF  +P ++P    +MLGRYVG+K+L +RS
Sbjct: 504 RVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERS 545

BLAST of CsaV3_2G008140 vs. Swiss-Prot
Match: sp|Q945K2|MDL2_PRUDU ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 5.6e-156
Identity = 267/524 (50.95%), Postives = 364/524 (69.47%), Query Frame = 0

Query: 8   QLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLER 67
           ++HS     + D SY+ F ++ATDL  +  YDY+I+GGGT+GCPLA TLS K+ VL+LER
Sbjct: 24  EVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLER 83

Query: 68  GSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSR 127
           GS P  YP+VL   G +      DDG+ P +RFVSEDG++N+RGRVLGG S++NAG Y+R
Sbjct: 84  GSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYAR 143

Query: 128 GHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDNGFDLR 187
            +   ++++GVDWDM+LV + YEWVE+T+V +P   +WQS  +++ LE GV P++GF L 
Sbjct: 144 ANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLD 203

Query: 188 HLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFS---GLSANGVLYS 247
           H  GT+  GS FDNKG RH A ELLNK N  N++V + A+V++I+FS   GL+A GV+Y 
Sbjct: 204 HEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYR 263

Query: 248 DSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGQSMS 307
           DS G  H+A +R KGE+IVSAG IG+PQLLLLSG+GP+S+LSSL +PVVL  PYVGQ + 
Sbjct: 264 DSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLH 323

Query: 308 DNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXXXXXT 367
           DNPR   NI+ P P+ P+ V V+GI  D  + Q   S  P   P               T
Sbjct: 324 DNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPPFGFFPSASYPLPNST 383

Query: 368 LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLLKTRT 427
            A F  K +   S GSL L SS+NV+ SP V FNYYS+  DL  CV G++K+G+LL T  
Sbjct: 384 FAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDA 443

Query: 428 MEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNY 487
           ++  K ++L G +GF  LG+PLP++  +D++ E +C+++V +YWHYHGGCLVGKV+DG++
Sbjct: 444 LKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDF 503

Query: 488 KVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRS 529
           +V GI  LRVVDGSTF  +P ++P    +MLGRYVG+K+L +RS
Sbjct: 504 RVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERS 545

BLAST of CsaV3_2G008140 vs. Swiss-Prot
Match: sp|P52707|MDL3_PRUSE ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 9.3e-151
Identity = 260/534 (48.69%), Postives = 361/534 (67.60%), Query Frame = 0

Query: 2   ISIFQLQLHSSHAIPN---QDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSS 61
           I +  LQ     ++ N    D SY+ FV++ATD   +  YDYII+GGGTAGCPLA TLS+
Sbjct: 15  IFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSA 74

Query: 62  KFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGS 121
            +SVL+LERGS P +YP++L   G +      DDG+ P +RFVSEDG++N+RGRVLGG S
Sbjct: 75  NYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTS 134

Query: 122 MVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGV 181
           M+NAG Y R +  FF   G++WDM+LV + YEWVE+T+V +P    WQ+   ++ LE G+
Sbjct: 135 MINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGI 194

Query: 182 VPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILF----S 241
           +P+NGF + HL GT+  GS FDN G RH + ELLNK +P N++VA++A V++I+F    S
Sbjct: 195 LPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTS 254

Query: 242 GLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVL 301
           G++A GV+Y+DS G  H+A +R +GE+I+SAG IGSPQLLLLSG+GP+S+L+SL + VV 
Sbjct: 255 GVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVA 314

Query: 302 HQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXX 361
             PYVGQ + DNPR   NI+ P P+  S+V V+GI  D  + Q   S  P   P      
Sbjct: 315 SHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFDTPPFSFFP 374

Query: 362 XXXXXXXXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVR 421
                    T A  V K     S G++ LNSS++V+  P V FNYYS+  DL  CV G++
Sbjct: 375 TTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMK 434

Query: 422 KMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGC 481
           K+G++L T  +E  K ++L G  GF  LG+PLPEN  +D++ E +C+++V +YWHYHGGC
Sbjct: 435 KLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGC 494

Query: 482 LVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRS 529
           LVGKV+D  ++V GI  LRVVDGSTF  +P ++P    +MLGRY+G+++L +RS
Sbjct: 495 LVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERS 546

BLAST of CsaV3_2G008140 vs. Swiss-Prot
Match: sp|O82784|MDL4_PRUSE ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.3e-149
Identity = 263/533 (49.34%), Postives = 355/533 (66.60%), Query Frame = 0

Query: 4   IFQLQLHSSHAIPN----QDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSK 63
           +  LQ    H++ N     D  Y+KFV+NA DL  +  YDYII+GGGT+GCPLA TLS+ 
Sbjct: 17  VLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTSGCPLAATLSAN 76

Query: 64  FSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSM 123
           +SVL+LERG+   +YP+ L   G        DDG+ P +RFVSEDG++N+R R+LGG ++
Sbjct: 77  YSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDNVRSRILGGTTI 136

Query: 124 VNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVV 183
           +NAG Y+R +  F+ ++GV+WD++LV +AYEWVE+ +V +P   +WQS   ++ LE GV 
Sbjct: 137 INAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQSWQSITGTAFLEAGVH 196

Query: 184 PDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILF----SG 243
           PDNGF L H  GT+  GS FDN G RH + ELLNK +P N+KVA+EA VQ+I+F    SG
Sbjct: 197 PDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVAVEAAVQKIIFSTESSG 256

Query: 244 LSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLH 303
           L+A GV+Y+DS G  HRA++  KGE+I+SAG +G+PQLLLLSG+GP+S+L+SL + VV  
Sbjct: 257 LTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGVGPESYLTSLNISVVAS 316

Query: 304 QPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXX 363
            PYVGQ ++DNPR   NI+ P P+ PS+V V+GI  D  + Q   S  P   P       
Sbjct: 317 HPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCSLSSLPFDTPPFSLFPT 376

Query: 364 XXXXXXXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRK 423
                   T A  V K     S GSL L SS+NV  +P V FNY S P DL  CV G++K
Sbjct: 377 TSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSDPVDLTHCVSGMKK 436

Query: 424 MGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCL 483
           +G  L T  ++  K  +L G  GF  LG PLPEN  +D++ E++C+ TV +YWHYHGG +
Sbjct: 437 IGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEKFCRDTVASYWHYHGGAI 496

Query: 484 VGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRS 529
           VGKV+DGN++V GI  LRVVDGSTF  +P ++P    +MLGRYVG K++ +RS
Sbjct: 497 VGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGRYVGTKIVQERS 547

BLAST of CsaV3_2G008140 vs. Swiss-Prot
Match: sp|O24243|MDL1_PRUDU ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 6.7e-149
Identity = 262/532 (49.25%), Postives = 352/532 (66.17%), Query Frame = 0

Query: 4   IFQLQLHSSHAIPN---QDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 63
           +  LQ    H++ N    D SY+KFV+NATD   +  YDYI+IGGGT+GCPLA TLS K+
Sbjct: 17  VLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATLSEKY 76

Query: 64  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 123
            VLLLERG+   +YP+ L   G        DDG+ P +RFVSEDG++N+R R+LGG +++
Sbjct: 77  KVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGIDNVRARILGGTTII 136

Query: 124 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 183
           NAG Y+R +  F++  G++WD++LV K YEWVE+ +V +P   +WQS      LE G++P
Sbjct: 137 NAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAGILP 196

Query: 184 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILF----SGL 243
           DNGF L H  GT+  GS FDN G RH A ELLNK +P N+ VA++A+V++ILF    S L
Sbjct: 197 DNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSNTSNL 256

Query: 244 SANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQ 303
           SA GV+Y+DS G  H+A +R  GE+IVSAG IG+PQLLLLSG+GP+S+LSSL + VV   
Sbjct: 257 SAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQPN 316

Query: 304 PYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXX 363
           PYVGQ + +NPR   N   P P+  S V V+GI  D  Y Q   S  P   P        
Sbjct: 317 PYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLPFSTPPFSLFPTT 376

Query: 364 XXXXXXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKM 423
                  T A  V +     S GS+ LNSS++V+ +P + FNYYS+  DL  CV G++K+
Sbjct: 377 SYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKL 436

Query: 424 GDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLV 483
           GDLL+T+ +E  K +++ G  GF +LGVPLPEN  +D+S E +C   V +YWHYHGG LV
Sbjct: 437 GDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGGSLV 496

Query: 484 GKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRS 529
           GKV+D +++V+GIK LRVVD STF   P ++P    +MLGRYVGL++L +RS
Sbjct: 497 GKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQILQERS 546

BLAST of CsaV3_2G008140 vs. TrEMBL
Match: tr|A0A0A0LK76|A0A0A0LK76_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 4.7e-293
Identity = 516/517 (99.81%), Postives = 517/517 (100.00%), Query Frame = 0

Query: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60
           MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 14  MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 73

Query: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120
           SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV
Sbjct: 74  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 133

Query: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180
           NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP
Sbjct: 134 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 193

Query: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240
           D+GFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG
Sbjct: 194 DSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 253

Query: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300
           VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG
Sbjct: 254 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 313

Query: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 360
           QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX
Sbjct: 314 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 373

Query: 361 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420
           XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL
Sbjct: 374 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 433

Query: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480
           KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV
Sbjct: 434 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 493

Query: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 518
           DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Sbjct: 494 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of CsaV3_2G008140 vs. TrEMBL
Match: tr|A0A1S3BSB5|A0A1S3BSB5_CUCME ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=1)

HSP 1 Score: 973.4 bits (2515), Expect = 2.1e-280
Identity = 482/530 (90.94%), Postives = 505/530 (95.28%), Query Frame = 0

Query: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60
           MISIFQLQLHSS AIPNQDV YMKFVHNATDLP KEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 14  MISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKF 73

Query: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120
           SVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRFVSEDG+ENIR RVLGGGSMV
Sbjct: 74  SVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIENIRARVLGGGSMV 133

Query: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180
           NAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGV+P
Sbjct: 134 NAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVIP 193

Query: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240
           DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EATVQRILFSGLSANG
Sbjct: 194 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEATVQRILFSGLSANG 253

Query: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300
           VLYSDSKGKLH+A IR+KGEII+SAGAIGSPQLLLLSGIGPKS+LSSLKLPVVLHQPYVG
Sbjct: 254 VLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPYVG 313

Query: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 360
           QSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASPFPIL+P IFS        
Sbjct: 314 QSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILVPPIFS-FLTQVTS 373

Query: 361 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420
              T A+FVGKFSEVHSEGSLRLNSSTNVK+SPIV FNYYSHPDDLGRCVKGVRK+GDLL
Sbjct: 374 ITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLGRCVKGVRKIGDLL 433

Query: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480
           KT+TMEKIKT+NLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TYWHYHGGC+VGKVV
Sbjct: 434 KTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTYWHYHGGCVVGKVV 493

Query: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNL 531
           DGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLK+L +R NL
Sbjct: 494 DGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKVLQERLNL 542

BLAST of CsaV3_2G008140 vs. TrEMBL
Match: tr|A0A1S3BS35|A0A1S3BS35_CUCME ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493093 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 1.2e-272
Identity = 470/530 (88.68%), Postives = 491/530 (92.64%), Query Frame = 0

Query: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60
           MISIFQLQLHSSHAIPNQDVSYMKFVHNATDL  KEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLSTKEEYDYIIIGGGTAGCPLATTLSSKF 60

Query: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120
           SVL+LERGSDPNKYP VL+E GL NVF AGDDG+NP QRF+SEDGVENIRGRVLGGGSMV
Sbjct: 61  SVLVLERGSDPNKYPFVLNELGLFNVFTAGDDGKNPSQRFISEDGVENIRGRVLGGGSMV 120

Query: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180
           NAGFYSRGHREFF SAGVDWDMELVEKAYEWVEETVVSQPILNAWQS FRSSLLE GVVP
Sbjct: 121 NAGFYSRGHREFFESAGVDWDMELVEKAYEWVEETVVSQPILNAWQSTFRSSLLESGVVP 180

Query: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240
           DNGFDLRHLVGTKTGGSIFDN GNRHGAVE LNKAN TNIKVAIEATVQRILFSGLSANG
Sbjct: 181 DNGFDLRHLVGTKTGGSIFDNIGNRHGAVEFLNKANTTNIKVAIEATVQRILFSGLSANG 240

Query: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300
           VLYSDSKGKLH+A IRKKGEII+SAGAIGSPQLLLLSGIGPKS+LSSLKLP+VLHQPYVG
Sbjct: 241 VLYSDSKGKLHKAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSYLSSLKLPLVLHQPYVG 300

Query: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 360
           QSMSDNPRFGT  I+PFPVLPSS K VGILQDNIYIQSIAS  PILIP IFS        
Sbjct: 301 QSMSDNPRFGTYFILPFPVLPSSAKAVGILQDNIYIQSIASLLPILIPPIFSFLPPGATS 360

Query: 361 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420
              +L + +GKFSEVHSEGSLRLNSST+VK+SPIV FNYYSHPDDLGRCVKGVRK+GDLL
Sbjct: 361 IDPSLVVLIGKFSEVHSEGSLRLNSSTDVKRSPIVRFNYYSHPDDLGRCVKGVRKIGDLL 420

Query: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480
           KT+TMEKIKT+NLE NKGFEFLGVPLPENL N S+VEEYCKKTV+TYWHYHGGC+VGKVV
Sbjct: 421 KTKTMEKIKTQNLEDNKGFEFLGVPLPENLGNVSAVEEYCKKTVSTYWHYHGGCVVGKVV 480

Query: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNL 531
           DGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLKLL QRSNL
Sbjct: 481 DGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKLLQQRSNL 530

BLAST of CsaV3_2G008140 vs. TrEMBL
Match: tr|A0A1S3BTS5|A0A1S3BTS5_CUCME ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 3.0e-255
Identity = 443/527 (84.06%), Postives = 475/527 (90.13%), Query Frame = 0

Query: 2   ISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFS 61
           ISIF LQL SS AIPNQDVSYMKFVHNATDLP KEEYDYIIIGGGTAGCPLATTLSSKFS
Sbjct: 15  ISIFPLQLLSSEAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFS 74

Query: 62  VLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVN 121
           +L+LERGSDPNKYPSVL+ QG+ N FAAGDDG+NPFQ FVSEDGVENIRGRVLGGGSMVN
Sbjct: 75  ILVLERGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSMVN 134

Query: 122 AGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPIL-NAWQSAFRSSLLEGGVVP 181
           AGFYSRGHREFF +AGVDWDMELVEKAYEWVEETVVSQPIL NAWQ+AFRS+LLEGGVVP
Sbjct: 135 AGFYSRGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGVVP 194

Query: 182 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 241
           DNGFDLRHLVGTK GGSIFDNKGNRHGAVELLNKANPTNIKVAI+ATVQ+ILFSGLSA G
Sbjct: 195 DNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVAIQATVQKILFSGLSATG 254

Query: 242 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 301
           V YSDSKGKLH A I K GEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQP+VG
Sbjct: 255 VSYSDSKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVG 314

Query: 302 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 361
           Q M+DNPRFGT I++PF +LPS+ KVVGILQ+NIYIQS A P   L+P  FS        
Sbjct: 315 QFMTDNPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPSTFLVPPAFSLLPPQSTS 374

Query: 362 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 421
              TLA FVGKFS+V+S+G L LNSS +VKKSPIV FNYYSH DDL RCV+GVRK+GDLL
Sbjct: 375 INPTLASFVGKFSDVNSKGWLHLNSSLDVKKSPIVRFNYYSHHDDLDRCVRGVRKVGDLL 434

Query: 422 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 481
           KT+T+EKIKT++LEG K F FLG+PL ENLWNDSSV+EYCKKTVTTYWHYHGGCLVGKVV
Sbjct: 435 KTQTIEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTVTTYWHYHGGCLVGKVV 494

Query: 482 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 528
           DGNYKVIGI+NLRVVDGSTFS SPGTNPMATLMMLGRYVGLK+L QR
Sbjct: 495 DGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLKQR 541

BLAST of CsaV3_2G008140 vs. TrEMBL
Match: tr|A0A1S3BSR3|A0A1S3BSR3_CUCME ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 1.9e-254
Identity = 433/529 (81.85%), Postives = 470/529 (88.85%), Query Frame = 0

Query: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60
           +ISIFQLQ+HSSHAIPNQ+VSYMKFVHN  DLP KE+YDYIIIGGGTAGCPLATTLSSKF
Sbjct: 15  IISIFQLQVHSSHAIPNQNVSYMKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKF 74

Query: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120
           SVLLLERGSDPNKYP VL+EQGL   F   DDG+ PFQRFVSEDGVENIRGRVLGGGSMV
Sbjct: 75  SVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMV 134

Query: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180
           N GFYSRGH+EFF SAGVDWDMELVEKAYEWVEETVVSQPIL  WQ+AFRS+LLEGG+VP
Sbjct: 135 NIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVP 194

Query: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240
           DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANP N+KVAIE TVQRILFS LSA G
Sbjct: 195 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKG 254

Query: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300
           V Y DS GKLH A I KKGEI++SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG
Sbjct: 255 VSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 314

Query: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSXXXXXXXX 360
           +SMSDNPRFG ++++ F V+P++ KVVGILQDNIY QS+A P P L+P  FS        
Sbjct: 315 KSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTS 374

Query: 361 XXXTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420
              TLA FVGKFSEV+S+GSL+LNSS +VKKSPIV FNYYSHP DL RCVK VRK+GD+ 
Sbjct: 375 INLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVF 434

Query: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480
           KT TMEKIKT++LEGNK F FLG+PLPENLWNDS+VEEYCKKTV TYWHYHGGCLVGKVV
Sbjct: 435 KTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVV 494

Query: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSN 530
           DGNYKVIGI+NLRVVDGSTFS SPGTNPMATLMMLGRYVGLK++ QRS+
Sbjct: 495 DGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQRSS 543