CsaV3_1G037470 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G037470 vs. NCBI nr
Match: XP_011659204.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 542/543 (99.82%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60
           MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS
Sbjct: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV
Sbjct: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDA+GVFHDRKSMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDAHGVFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300

Query: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360
           QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED
Sbjct: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360

Query: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420
           QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS
Sbjct: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420

Query: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480
           QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV
Sbjct: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480

Query: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLK 540
           NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLK
Sbjct: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLK 540

Query: 541 HKI 544
           HKI
Sbjct: 541 HKI 543

BLAST of CsaV3_1G037470 vs. NCBI nr
Match: XP_008445709.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1040.0 bits (2688), Expect = 2.8e-300
Identity = 506/543 (93.19%), Postives = 526/543 (96.87%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60
           MKFSLLLPLSLAFI VCSSSSVWPSASSHVNHEEFL CLLHHSP SKSI KL YTPINTS
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YS VLNFSIRNLRFS PNTPKPL IITP+NISHVQAAVICSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYHPF++VDLI+LSSVTIEV+QSTAWVQSGATLGELYYRIAEKSRTLAFPAGN PTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGFGTLLRKYGLAADNVIDAY+VDANG FHDRKSMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTICTISRTLEEEAIKL+DQWQYVANKLDEDLFLGINLLGGKI A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360
           QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED
Sbjct: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360

Query: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420
           QPLEVLLNRTPLA GNIKMKSDYVKEPI KATI+E+WQRL+SQDIEGANLVF+PYGGRMS
Sbjct: 361 QPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRMS 420

Query: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480
           QIS+SEIPFSHRAGNLYKIGYLTGWFEPG+NAEKTHLNWIRDIY YMTPFV+KSPRAAYV
Sbjct: 421 QISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYV 480

Query: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLK 540
           NYRDLDIGSNSKYGKTSYKRA VWG KYFG+NF+R+VYVKN+VDPYNFFRHEQSIPVLLK
Sbjct: 481 NYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLK 540

Query: 541 HKI 544
            +I
Sbjct: 541 RRI 543

BLAST of CsaV3_1G037470 vs. NCBI nr
Match: XP_011659211.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 865.9 bits (2236), Expect = 7.2e-248
Identity = 417/544 (76.65%), Postives = 475/544 (87.32%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60
           MKFS L+PLSL F+ VCSSSS+W +ASS +NHEEF+QCLLHHSP +  I KL +TPIN+S
Sbjct: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           +SS+LNF  RN RFS PNTPKPL IITP+NISH+QAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYH FI+VDLI+L SVT++V+QSTAWV+SGATLGELYY+I +KSRTL FPAG  PTV
Sbjct: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKL+DQWQYVANKL+E+LFL I   GGK+SA
Sbjct: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300

Query: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360
           Q G KINPIALFF+LFLG  +ELMAIL+KTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 QEGGKINPIALFFTLFLGNVNELMAILSKTFPELGLTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRM 420
           DQPLE LLNRTP+  G+ K+KSDYVKEP+ KA I+ IW+RLESQDIEG  L  +PYGGRM
Sbjct: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420

Query: 421 SQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 480
           +QIS+S+IPF HRAG LY+IGY+ GW E GV AEK HLNWIR+IY YMTPFVSKSPRAAY
Sbjct: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLL 540
           VNYRDLDIGSN+KYGK +YK+A V+G KYFGNNFNR+V VK+ VDPYNFF HEQSIP+  
Sbjct: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS 540

Query: 541 KHKI 544
            H +
Sbjct: 541 DHMV 544

BLAST of CsaV3_1G037470 vs. NCBI nr
Match: XP_008445721.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 845.5 bits (2183), Expect = 1.0e-241
Identity = 412/539 (76.44%), Postives = 462/539 (85.71%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60
           M  S L+PLSL FI VCSSSS+W + SS + HEEFLQCLLHHSP + SI KL +TPIN  
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINXX 73

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
            S++ NF  RN RFS PNTPKPL IITP+NISH+QAAVICSKSHGLQIRIRSGGHDFEGL
Sbjct: 74  XSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYH FI+VDLI+L SVT++V+QSTAWVQSGATLGELYY+I +KSRTL FPAG  PTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           +VVAWKVKLVPVPA+VTIC+  R++EEEAIKL+D+WQYVANKL+EDLFL I   GGK+SA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360
           Q G KINPIALFF LFLG  +ELMAILNKTFP+LG TKEECKE SWIES     N  QIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEVLLNRTPLATG-NIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
           DQPLE LLNRTP+  G + K+KSDYVKEP+ K  IE IW+RLESQDIEG  L  +PYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           MS+IS+S IPFSHRAG LY+IGY+ GW E GV AE+ HLNWIR+IY YMTPFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPV 538
           YVNYRDLDIGSN+KYGK +YK+A V+G KYFGNNFNR+V VK KVDPYNFF HEQSIP+
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPL 552

BLAST of CsaV3_1G037470 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 797.3 bits (2058), Expect = 3.1e-227
Identity = 395/538 (73.42%), Postives = 461/538 (85.69%), Query Frame = 0

Query: 4   SLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPH--SKSIAKLAYTPINTSY 63
           S L+ LSLAFI + SSSS+W SA  +  HE FLQCLL HS    + SI+K+ +TPIN+SY
Sbjct: 5   SPLISLSLAFIFL-SSSSLWASAFPN-KHESFLQCLLDHSLEYSNYSISKVIHTPINSSY 64

Query: 64  SSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLS 123
           SSVL+FSIRNLRFS   TPKPL I+TP+++SH+QAAVICSKS+G QIR RSGGHDFEGLS
Sbjct: 65  SSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLS 124

Query: 124 YVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVG 183
           YVA+ PFIIVDLI+L S++I++K +TAWVQSGATLGELYY IA+KSRTLAFPAG  PTVG
Sbjct: 125 YVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVG 184

Query: 184 VGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGI 243
           VGGHFSGGG+G LLRKYGLAADNVIDAYLVDA+G FH+R+SMGEDLFWAIRGGGGGSFGI
Sbjct: 185 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGI 244

Query: 244 VVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQ 303
           VVAWKVKLVPVPATVT+C+ +RTLEE A+K+  +WQYVANKLDE+LFLGI  LGGKI++Q
Sbjct: 245 VVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQ 304

Query: 304 -GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 363
            G K NP+A FFSLFLGKADEL++ILN  FP+L L K++C ETSWIES V  G G   E 
Sbjct: 305 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTE- 364

Query: 364 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 423
             LE LL+RTPL   + K+KSDYVKEP+ +A ++ IW+RL+SQDIEG N++FVPYGGRMS
Sbjct: 365 LTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMS 424

Query: 424 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 483
           QIS+SE PFSHRAG LYKIGY+ GW E G++AEK HL WIR++Y YMTPFVSKSPRAAY 
Sbjct: 425 QISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYA 484

Query: 484 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVL 539
           NYRDLDIGSN+K+GKTSYK+A VWGLKYFGNNFNR+V+VK KVDPY+FFRHEQSIP L
Sbjct: 485 NYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of CsaV3_1G037470 vs. TAIR10
Match: AT5G44410.1 (FAD-binding Berberine family protein)

HSP 1 Score: 525.8 bits (1353), Expect = 3.2e-149
Identity = 263/540 (48.70%), Postives = 371/540 (68.70%), Query Frame = 0

Query: 6   LLPLSLAFIAVCSSSSVWPSASS-HVNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYSSV 65
           LL L + F+ +  S S +PS S+   NHE FL+CL H      S  ++ +T  + SY S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSI 66

Query: 66  LNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVA 125
           LN SI+N RF +  TPKP+ IITP   + VQ+ + C++ HG+ IR RSGGHD+EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 -YHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVG 185
              PF+++DL +L S+T++V   T WVQSGAT+GELYY I + S++LAFPAG  PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVV 245
           G F GGG+GTL+RKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGD 305
           +WK++L+ VP+ VT+  + +T E+EA+ ++++WQY+A+K+  DLF+   L          
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML--------QK 306

Query: 306 KINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPL 365
           +    A F  L+LG   +L+A++   FP+LGL    C+E SWIESV++      I+ + +
Sbjct: 307 ETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWF-----IKGESM 366

Query: 366 EVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQIS 425
           E+L  R    + + K K D+++EPIPK  I+ +W+R E+ +   A ++  P+GG+MS+I+
Sbjct: 367 EILAKR-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIA 426

Query: 426 DSEIPFSHRAGNLYKIGYLTGWFEP----GVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 485
           D+EIPF HR GNLY+I YL  W E       N EK +L W+  +Y +MTP+VSKSPR AY
Sbjct: 427 DNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEK-YLRWVESVYEFMTPYVSKSPRRAY 486

Query: 486 VNYRDLDIGSNSKYG-KTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVL 539
           VN+RD+D+G       KT Y+ A+VWG+KYF NNF+R+V VK  VDP +FF  EQSIP++
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of CsaV3_1G037470 vs. TAIR10
Match: AT4G20820.1 (FAD-binding Berberine family protein)

HSP 1 Score: 524.6 bits (1350), Expect = 7.1e-149
Identity = 260/538 (48.33%), Postives = 368/538 (68.40%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCS--SSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPIN 60
           MKF       L F    +   S   P +++  N   FLQCL      S  ++K+ +TP +
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  TSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFE 120
           TS+SSVL  SI+N RFS P+ PKP+ I+TP   S VQ+AV C++  G+ IR RSGGHD+E
Sbjct: 61  TSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYE 120

Query: 121 GLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSP 180
           GLSYV + PF+I+DL +L S+T++V   + WVQ+GAT+GELYY I +K+RTLAFPAG  P
Sbjct: 121 GLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCP 180

Query: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGS 240
           TVGVGGHFSGGG+GTLLRK+GLAAD+VIDA +VDA G   +R+ MGED FWAIRGGGG S
Sbjct: 181 TVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSS 240

Query: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKI 300
           F +V++WK+ L+ VP+TVT+  +++  E+ A+K++ +WQ+VA+K+ +DLF+       ++
Sbjct: 241 FCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFI-------RV 300

Query: 301 SAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360
             Q  K    A F  L+LG    L+ ++NK FP+LGL +++C E SWIESV++     ++
Sbjct: 301 MLQRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---EL 360

Query: 361 EDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
            ++P+ VL  RT  A+   K KSD+V+EP+PK  I ++W+RL+  + E A L+F P+GG+
Sbjct: 361 GEEPINVLTKRT-RASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGK 420

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           MS+I+D E PF HR GN+Y+I YL  W     + ++ ++ W+  +Y  M+ FV+KSPR A
Sbjct: 421 MSEIADYETPFPHRKGNIYEIQYLNYW---RGDVKEKYMRWVERVYDDMSEFVAKSPRGA 480

Query: 481 YVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIP 537
           Y+N RDLD+G      ++ Y+  + WG+KYF NNF R+V VK  VDP +FF  EQSIP
Sbjct: 481 YINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CsaV3_1G037470 vs. TAIR10
Match: AT5G44440.1 (FAD-binding Berberine family protein)

HSP 1 Score: 522.3 bits (1344), Expect = 3.5e-148
Identity = 258/536 (48.13%), Postives = 374/536 (69.78%), Query Frame = 0

Query: 10  SLAFIAVCSSSSVWPSASSH-VNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFS 69
           S  F  +  S ++ P  S+H  NHE+FL+CL +    +    K+ +T  ++S+ S+L+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 70  IRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYH-P 129
           I+N RFS+  TPKP+ IITP   S VQ  + C++ HG+ +R RS GH +EGLSY+AY+ P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 130 FIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFS 189
           F ++DL +L S++++V   T WVQ+GAT GELYY I + +++LAFPAG  PTVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 190 GGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKV 249
           GGG+GTLLRKYGLAADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 250 KLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGDKINP 309
           KLV VP+T+T+  + +T ++EA++++ +WQY A+K+ +DLF+   L       + +K   
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL------ERSNKNAV 304

Query: 310 IALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLL 369
            ALF  L++G  + L+A++ + FP+LGL KE C+E SWIESV++  +  + E   L VL 
Sbjct: 305 HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGES--LGVLT 364

Query: 370 NRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEI 429
           NR   +  + K K D+V+EPIP+A I+EIW+RLE+ +     ++  P+GG+MS++++ E 
Sbjct: 365 NRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 424

Query: 430 PFSHRAGNLYKIGYLTGW-FEPGVNAEKT--HLNWIRDIYGYMTPFVSKSPRAAYVNYRD 489
           PF HR GNLY+I Y+  W  E   N  +T  +L W+  +Y +MTP+VSKSPR AYVN++D
Sbjct: 425 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 490 LDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLK 541
           +D+G      KT Y+  + WG+KYF NNF R+V VK +VDP +FF  EQSIP++ K
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVNK 531

BLAST of CsaV3_1G037470 vs. TAIR10
Match: AT5G44400.1 (FAD-binding Berberine family protein)

HSP 1 Score: 511.9 bits (1317), Expect = 4.8e-145
Identity = 255/544 (46.88%), Postives = 370/544 (68.01%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPIN-- 60
           M  S  LPL    +   S  ++ P++S     ++F+ C+  ++     + K  + P    
Sbjct: 1   MGISKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNV 60

Query: 61  TSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFE 120
           + +S VL  + +NLRF   + PKP FI +P + SHVQA++ICSK   + +R+RSGGHD+E
Sbjct: 61  SMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYE 120

Query: 121 GLSYVAY--HPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGN 180
           GLSYV+    PFI++DL  +  V I ++ ++AWVQSGAT+GELYYRIAEKS+   FPAG 
Sbjct: 121 GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGL 180

Query: 181 SPTVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGG 240
             ++G+GGH +GG +G+++RKYGL ADNV+DA +VDANG   DR +MGED FWAIRGG G
Sbjct: 181 CSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAG 240

Query: 241 GSFGIVVAWKVKLVPVPATVTICTISRTLEEE-AIKLVDQWQYVANKLDEDLFLGINLLG 300
           GSFGI++AWK+KLVPVP TVT+ T+++TL+++   K++ +WQ VA+KL E+LF+ +    
Sbjct: 241 GSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF-- 300

Query: 301 GKISAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNG 360
             ++  G        + +LFLG    LM ++ K+FP+LGLT ++C E SW+ES+ Y  +G
Sbjct: 301 -NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYI-SG 360

Query: 361 LQIEDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPY 420
                    +L  ++P    + K KSD+VK PIP++ ++ I+++L  +DI    +++ PY
Sbjct: 361 FPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPY 420

Query: 421 GGRMSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSP 480
           GG M++I +S+IPF HR G L+K+ Y+T W +      + H+NWIRD+Y YMTP+VS +P
Sbjct: 421 GGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSR-HINWIRDLYSYMTPYVSSNP 480

Query: 481 RAAYVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSI 540
           R AYVNYRDLD+G N+K  KT  K+A+VWG  YF NNFNR++ +K KVDP NFFRHEQSI
Sbjct: 481 REAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSI 537

BLAST of CsaV3_1G037470 vs. TAIR10
Match: AT1G30700.1 (FAD-binding Berberine family protein)

HSP 1 Score: 511.5 bits (1316), Expect = 6.2e-145
Identity = 268/543 (49.36%), Postives = 363/543 (66.85%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60
           MK++L+L L   F  V     +W S+SS  N E F QCL  +S     I+   +   N S
Sbjct: 1   MKYALILVL---FFVV----FIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YSSVL  +IRNLRF+  +TPKP  II  T+ SHVQAA+ C K H LQ++IRSGGHD++GL
Sbjct: 61  YSSVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGL 120

Query: 121 SYVAY--HPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSP 180
           SYV Y   PF ++D+ +L SV ++V   TAWVQ+GA LGE+YY I EKS+TLA+PAG  P
Sbjct: 121 SYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICP 180

Query: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGS 240
           TVGVGGH SGGG+G ++RKYGL  DN IDA +VD NG   DRK MGEDL+WAI GGGGGS
Sbjct: 181 TVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGS 240

Query: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLG--INLLGG 300
           +G+V+A+K+ LV VP  VT+  ISRTLE+ A  ++ +WQ VA KL ++LF+   I+++ G
Sbjct: 241 YGVVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNG 300

Query: 301 KISAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGL 360
            +S+Q         F ++FLG    L++ILN+ FP+LGL + +C ETSWI+SV++  N  
Sbjct: 301 TVSSQK---TVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN-- 360

Query: 361 QIEDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYG 420
            I+    E LL +       +K KSDYV+EPI +  +E IW+++   ++E   + F PYG
Sbjct: 361 -IQVGSSETLLLQRNQPVNYLKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPYG 420

Query: 421 GRMSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPR 480
           G M +IS +  PF +RAGNL+KI Y   W +  +     ++   R +Y +MTPFVSK+PR
Sbjct: 421 GEMGRISSTVTPFPYRAGNLWKIQYGANWRDETLT--DRYMELTRKLYQFMTPFVSKNPR 480

Query: 481 AAYVNYRDLDIGSNSKYGK-TSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSI 539
            ++ NYRD+D+G NS  GK +SY   + +G KYF  NF R+V +K +VD  NFFR+EQSI
Sbjct: 481 QSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSI 526

BLAST of CsaV3_1G037470 vs. Swiss-Prot
Match: sp|A6P6V9|CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.3e-149
Identity = 259/515 (50.29%), Postives = 360/515 (69.90%), Query Frame = 0

Query: 33  EEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNIS 92
           E FL+C   + P++ +  KL YT  N  Y SVLN +I NLRF+   TPKPL I+TP+++S
Sbjct: 32  ENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVS 91

Query: 93  HVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQS 152
           H+Q  ++CSK  GLQIR RSGGHD EG+SY++  PF+IVDL ++ S+ I+V   TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY + EK+  L+  AG  PTV  GGHF GGG+G L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVN 211

Query: 213 ANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE-EEAIK 272
            +G   DRKSMGEDLFWA+RGGG  SFGI+VAWK++LV VP + T+ ++ + +E  E +K
Sbjct: 212 VHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIMEIHELVK 271

Query: 273 LVDQWQYVANKLDEDLFLGINLLGGKIS-AQGDKINPIALFF-SLFLGKADELMAILNKT 332
           LV++WQ +A K D+DL L  + +   I+  QG     I  +F S+FLG  D L+ ++NK+
Sbjct: 272 LVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKS 331

Query: 333 FPQLGLTKEECKETSWIESVVYTGNGLQIEDQPL--EVLLNRTPLATGNIKMKSDYVKEP 392
           FP+LG+ K +C++ SWI+++++    +  +      E+LL+R+    G  K+K DYVK+P
Sbjct: 332 FPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKP 391

Query: 393 IPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEIPFSHRAGNLYKIGYLTGWFE 452
           IP++   +I ++L  +DI        PYGG M +IS+S IPF HRAG LY++ Y+  W +
Sbjct: 392 IPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEK 451

Query: 453 PGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNSKYGKTSYKRARVWGLK 512
              N  + HLNWIR+IY +MTP+VSK+PR AY+NYRDLDIG N      +Y +AR+WG K
Sbjct: 452 QEDN--EKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGEK 511

Query: 513 YFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLKHK 543
           YFG NF+R+V VK  VDP NFFR+EQSIP L +H+
Sbjct: 512 YFGKNFDRLVKVKTLVDPNNFFRNEQSIPPLPRHR 543

BLAST of CsaV3_1G037470 vs. Swiss-Prot
Match: sp|A6P6W0|CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 259/515 (50.29%), Postives = 359/515 (69.71%), Query Frame = 0

Query: 33  EEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNIS 92
           E FL+C   + P + + AKL YT  +  Y S+LN +I+NLRF+   TPKPL IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQS 152
           H+Q  ++CSK  GLQIR RSGGHD EG+SY++  PF+IVDL ++ SV I+V   TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +  L+FPAG  PTVG GGHFSGGG+G L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE-EEAIK 272
            +G   DRKSMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI ++ + +E  E +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LVDQWQYVANKLDEDLFLGINLLGGKIS-AQGDKINPIALFF-SLFLGKADELMAILNKT 332
           LV++WQ +A   +++L L  + +   I+  QG     I  +F S+F G  D L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPQLGLTKEECKETSWIESVVYTGNGLQIEDQ--PLEVLLNRTPLATGNIKMKSDYVKEP 392
           FP+LG+ K +CK+ SWI+++++    +         E+LL+R+        +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 IPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEIPFSHRAGNLYKIGYLTGWFE 452
           IP+  +  I ++L  +D+     VF PYGG M +IS+S IPF HRAG +Y+I Y+  W +
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 453 PGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNSKYGKTSYKRARVWGLK 512
              N  + H+NWIR++Y + TP+VS++PR AY+NYRDLD+G  +     +Y +AR+WG K
Sbjct: 452 QEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRMVYVKNKVDPYNFFRHEQSIPVL-LKH 542
           YFG NFNR+V VK KVDP NFFR+EQSIP L L+H
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPLRH 544

BLAST of CsaV3_1G037470 vs. Swiss-Prot
Match: sp|A6P6W1|CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 259/515 (50.29%), Postives = 358/515 (69.51%), Query Frame = 0

Query: 33  EEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNIS 92
           E FL+CL  + P + + AKL YT  +  Y S+LN +++NLRF+   TPKPL I TP N+S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 93  HVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQS 152
           H+Q  ++CSK  GLQIR RSGGHD EG+SY++  PF+IVDL ++ SV I+V   TAWV+S
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +  L+FPAG  PTVG GGHFSGGG+G L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE-EEAIK 272
            +G   DRKSMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI ++ + +E  E +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LVDQWQYVANKLDEDLFLGINLLGGKIS-AQGDKINPIALFF-SLFLGKADELMAILNKT 332
           LV++WQ +A   +++L L  + +   I+  QG     I  +F S+F G  D L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPQLGLTKEECKETSWIESVVYTGNGLQIEDQPL--EVLLNRTPLATGNIKMKSDYVKEP 392
           FP+LG+ K +CK+ SWI+++++    +         E+LL+R+        +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 IPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEIPFSHRAGNLYKIGYLTGWFE 452
           IP+  +  I ++L  +D+     VF PYGG M +IS+S IPF HRAG  Y+I Y+  W +
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEK 451

Query: 453 PGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNSKYGKTSYKRARVWGLK 512
              N  + H+NWIR++Y + TP+VS++PR AY+NYRDLD+G  +     +Y +AR+WG K
Sbjct: 452 QEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRMVYVKNKVDPYNFFRHEQSIPVL-LKH 542
           YFG NFNR+V VK KVDP NFFR+EQSIP L L+H
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPLRH 544

BLAST of CsaV3_1G037470 vs. Swiss-Prot
Match: sp|Q8GTB6|THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 255/514 (49.61%), Postives = 352/514 (68.48%), Query Frame = 0

Query: 33  EEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNIS 92
           E FL+C   H P++ +  KL YT  +  Y S+LN +I+NLRF    TPKPL I+TP+N S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 93  HVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQS 152
           H+QA ++CSK  GLQIR RSGGHD EG+SY++  PF++VDL ++ S+ I+V   TAWV++
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I EK+  L+FP G  PTVGVGGHFSGGG+G L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE-EEAIK 272
            +G   DRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  TI ++ + +E    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 273 LVDQWQYVANKLDEDLFLGINLLGGKISAQ--GDKINPIALFFSLFLGKADELMAILNKT 332
           L ++WQ +A K D+DL L  + +   I+     +K      F S+F G  D L+ ++NK+
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPQLGLTKEECKETSWIESVVYTGNGLQIEDQPL--EVLLNRTPLATGNIKMKSDYVKEP 392
           FP+LG+ K +CKE SWI++ ++    +         E+LL+R+        +K DYVK+P
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 393 IPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEIPFSHRAGNLYKIGYLTGWFE 452
           IP+  + +I ++L  +D+     V  PYGG M +IS+S IPF HRAG +Y++ Y   W +
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

Query: 453 PGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNSKYGKTSYKRARVWGLK 512
              N  + H+NW+R +Y + TP+VS++PR AY+NYRDLD+G  +     +Y +AR+WG K
Sbjct: 452 QEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511

Query: 513 YFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLKH 542
           YFG NFNR+V VK KVDP NFFR+EQSIP L  H
Sbjct: 512 YFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPPH 543

BLAST of CsaV3_1G037470 vs. Swiss-Prot
Match: sp|Q9FI25|BBE27_ARATH (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 5.8e-148
Identity = 263/540 (48.70%), Postives = 371/540 (68.70%), Query Frame = 0

Query: 6   LLPLSLAFIAVCSSSSVWPSASS-HVNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYSSV 65
           LL L + F+ +  S S +PS S+   NHE FL+CL H      S  ++ +T  + SY S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSI 66

Query: 66  LNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVA 125
           LN SI+N RF +  TPKP+ IITP   + VQ+ + C++ HG+ IR RSGGHD+EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 -YHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVG 185
              PF+++DL +L S+T++V   T WVQSGAT+GELYY I + S++LAFPAG  PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVV 245
           G F GGG+GTL+RKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGD 305
           +WK++L+ VP+ VT+  + +T E+EA+ ++++WQY+A+K+  DLF+   L          
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML--------QK 306

Query: 306 KINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPL 365
           +    A F  L+LG   +L+A++   FP+LGL    C+E SWIESV++      I+ + +
Sbjct: 307 ETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWF-----IKGESM 366

Query: 366 EVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQIS 425
           E+L  R    + + K K D+++EPIPK  I+ +W+R E+ +   A ++  P+GG+MS+I+
Sbjct: 367 EILAKR-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIA 426

Query: 426 DSEIPFSHRAGNLYKIGYLTGWFEP----GVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 485
           D+EIPF HR GNLY+I YL  W E       N EK +L W+  +Y +MTP+VSKSPR AY
Sbjct: 427 DNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEK-YLRWVESVYEFMTPYVSKSPRRAY 486

Query: 486 VNYRDLDIGSNSKYG-KTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVL 539
           VN+RD+D+G       KT Y+ A+VWG+KYF NNF+R+V VK  VDP +FF  EQSIP++
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of CsaV3_1G037470 vs. TrEMBL
Match: tr|A0A1S3BCV7|A0A1S3BCV7_CUCME (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 1.8e-300
Identity = 506/543 (93.19%), Postives = 526/543 (96.87%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60
           MKFSLLLPLSLAFI VCSSSSVWPSASSHVNHEEFL CLLHHSP SKSI KL YTPINTS
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YS VLNFSIRNLRFS PNTPKPL IITP+NISHVQAAVICSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYHPF++VDLI+LSSVTIEV+QSTAWVQSGATLGELYYRIAEKSRTLAFPAGN PTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGFGTLLRKYGLAADNVIDAY+VDANG FHDRKSMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTICTISRTLEEEAIKL+DQWQYVANKLDEDLFLGINLLGGKI A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360
           QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED
Sbjct: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360

Query: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420
           QPLEVLLNRTPLA GNIKMKSDYVKEPI KATI+E+WQRL+SQDIEGANLVF+PYGGRMS
Sbjct: 361 QPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRMS 420

Query: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480
           QIS+SEIPFSHRAGNLYKIGYLTGWFEPG+NAEKTHLNWIRDIY YMTPFV+KSPRAAYV
Sbjct: 421 QISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYV 480

Query: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLK 540
           NYRDLDIGSNSKYGKTSYKRA VWG KYFG+NF+R+VYVKN+VDPYNFFRHEQSIPVLLK
Sbjct: 481 NYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLK 540

Query: 541 HKI 544
            +I
Sbjct: 541 RRI 543

BLAST of CsaV3_1G037470 vs. TrEMBL
Match: tr|A0A1S3BDD9|A0A1S3BDD9_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 6.6e-242
Identity = 412/539 (76.44%), Postives = 462/539 (85.71%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60
           M  S L+PLSL FI VCSSSS+W + SS + HEEFLQCLLHHSP + SI KL +TPIN  
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINXX 73

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
            S++ NF  RN RFS PNTPKPL IITP+NISH+QAAVICSKSHGLQIRIRSGGHDFEGL
Sbjct: 74  XSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYH FI+VDLI+L SVT++V+QSTAWVQSGATLGELYY+I +KSRTL FPAG  PTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           +VVAWKVKLVPVPA+VTIC+  R++EEEAIKL+D+WQYVANKL+EDLFL I   GGK+SA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360
           Q G KINPIALFF LFLG  +ELMAILNKTFP+LG TKEECKE SWIES     N  QIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEVLLNRTPLATG-NIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
           DQPLE LLNRTP+  G + K+KSDYVKEP+ K  IE IW+RLESQDIEG  L  +PYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           MS+IS+S IPFSHRAG LY+IGY+ GW E GV AE+ HLNWIR+IY YMTPFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPV 538
           YVNYRDLDIGSN+KYGK +YK+A V+G KYFGNNFNR+V VK KVDPYNFF HEQSIP+
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPL 552

BLAST of CsaV3_1G037470 vs. TrEMBL
Match: tr|A0A1S3BDZ5|A0A1S3BDZ5_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 9.3e-220
Identity = 386/540 (71.48%), Postives = 441/540 (81.67%), Query Frame = 0

Query: 1   MKFSL-LLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINT 60
           MK+S  L+P SL  I V SSSS W SAS H  H+ FLQCL  HS     I+K+ +TPIN+
Sbjct: 1   MKYSSPLIPFSLILILVSSSSS-WVSASPH-KHQNFLQCLSEHSSKRYPISKVVHTPINS 60

Query: 61  SYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEG 120
           SYSSVL+FSIRNL FS P TPKPL IITP+++SH+QAAVICSKSHGLQIR RSGGHDFEG
Sbjct: 61  SYSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEG 120

Query: 121 LSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPT 180
           LSYVAYHPFIIVDLI+L S+ I+V+ +TAWV+SGATLGELYYRI EKSRTLAFP G  PT
Sbjct: 121 LSYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPT 180

Query: 181 VGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGG+G +LRK+GLAADNVIDAYLVDANG  HDRKSMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKIS 300
           GIVVAWK+KLV VPATVTIC+ +R L+ + IKLV QWQYVANKLDE+LFLGI L GG I+
Sbjct: 241 GIVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNIT 300

Query: 301 AQGDKI-NPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360
              + I NPIA FFSLFLGK D L+A L+  FP+LG TKE+C ETSWIES +    G+  
Sbjct: 301 TTQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGT 360

Query: 361 EDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
            +  LE LLNRTP    N KMKSDY KEPI  ATIE +WQRL+++DIE + L+F+PYGGR
Sbjct: 361 VES-LEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGR 420

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           MSQIS+ E PFSHRAG LYKIGY   W E  + A+K H++WIRDIY YMTPFVSKSPR+A
Sbjct: 421 MSQISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSA 480

Query: 481 YVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVL 539
           Y NYRDLDIG+N KYGKTS ++A +WGLKYFGNNF R+VYVK KVDPY+FFRHEQSIP L
Sbjct: 481 YANYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of CsaV3_1G037470 vs. TrEMBL
Match: tr|A0A1S3BDB7|A0A1S3BDB7_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488652 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 1.3e-218
Identity = 374/536 (69.78%), Postives = 435/536 (81.16%), Query Frame = 0

Query: 4   SLLLPL-SLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYS 63
           S L PL  +  I V SSS +W SA  H  H+ FL CL  HS  S  I+K+ YTPIN+SYS
Sbjct: 5   SQLTPLFHVLIIVVVSSSPLWVSAFPH-EHQNFLHCLPEHSSKSYPISKVVYTPINSSYS 64

Query: 64  SVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSY 123
           SVLNFSIRN RFS P TPKPL IITP+++SH+QAAVICSKSHGLQIR RSGGHD+EGLSY
Sbjct: 65  SVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSY 124

Query: 124 VAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGV 183
           VAY PFIIVDLI++ S+++++  +TAWV+SGATLGELYYRI EKSRTLAFPAG  PTVGV
Sbjct: 125 VAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGV 184

Query: 184 GGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIV 243
           GGHFSGGG+G LLRKYGLAADNVIDAYLVDANG+ HDR+SMGEDLFWAIRGGGGGSFGIV
Sbjct: 185 GGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSFGIV 244

Query: 244 VAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQG 303
           VAWKVKLVPVPA VTIC+  + LE +A+K++ QWQYVANKL ED+FLGI L GG  S QG
Sbjct: 245 VAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQG 304

Query: 304 DKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQP 363
               P A F+SLFLGK DEL+A L+ TFP+ GL K++C E  WIES +   +G+    + 
Sbjct: 305 GIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQTVES 364

Query: 364 LEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQI 423
           L+ LLNRTP    + K+KSDY+K+PIPKA IE IWQRL++ DIE   LVFVPYG RMSQI
Sbjct: 365 LKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARMSQI 424

Query: 424 SDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNY 483
           S+SE PFSHRAG LYKIGY+ GW    + A+  H++W R++Y YMTPFVSKSPR AYVNY
Sbjct: 425 SESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAYVNY 484

Query: 484 RDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVL 539
           RDLDIGSN+KYGKTSYK+A +WGLKYFGNNF R+VYVKNKVDP++FFRHEQSIP L
Sbjct: 485 RDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of CsaV3_1G037470 vs. TrEMBL
Match: tr|A0A1S3BDA8|A0A1S3BDA8_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 5.1e-218
Identity = 378/539 (70.13%), Postives = 444/539 (82.37%), Query Frame = 0

Query: 1   MKF-SLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINT 60
           MKF S L+PLSL FI V SS+S+  SAS    ++ FLQCL  HS  S  I+K+ YTPIN+
Sbjct: 1   MKFSSTLIPLSLVFIVV-SSASLCASASPQ-KYQNFLQCLSEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEG 120
           SYSSVL+FSIRNL FS P TPKPL IITP+++SH+QAAVICSKSHGLQIR RSGGHDFEG
Sbjct: 61  SYSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEG 120

Query: 121 LSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPT 180
           LSYVAY PFI+VDLI+L SV +EV+ +TAWV+SGATLGELYYRI EKSRTLAFPAG  PT
Sbjct: 121 LSYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSF 240
           VG+GGHFSGGG+G +LRK+GLAADNVIDAYLVDANG   D++SMGEDLFWAIRGGGGGSF
Sbjct: 181 VGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKIS 300
           GIVVAWK+KLVPVP TVTIC+  R LEE+AIKL+ +WQYV NKLDE+++LGI L GG  S
Sbjct: 241 GIVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAS 300

Query: 301 AQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360
            Q    NP A FFSLFLG+ADEL+  L+  FP+LGL K++C E SW+ES +    G+Q  
Sbjct: 301 TQAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQ-P 360

Query: 361 DQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRM 420
            + LE LLNRT     + K+KSDY+ EPIP A I++IWQRL++QDIE + LVFVPYGGRM
Sbjct: 361 IETLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRM 420

Query: 421 SQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 480
           SQIS+SE PFSHRA  L+KIGY+ GW +  + A+K H++W+R++Y YM PFVSKSPRAAY
Sbjct: 421 SQISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVL 539
            NYRDLDIGSN+KYGKTSYKRA VWGLKYFGNNF+R+VYVK KVDPY+FFRHEQSIP L
Sbjct: 481 TNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536