CsaV3_1G015340 (gene) Cucumber (Chinese Long) v3

NameCsaV3_1G015340
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
DescriptionSubtilisin-like protease
Locationchr1 : 10916683 .. 10920158 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTCTTTATTTCTCCTCTCATTTTCTTCTCATTTCTTTTGTTAATATCACCTGCCATTGCTACCAAAAAGGTAAATCTATTTTTTCTTTGGTTAACTCTCTTTCGTTTTTCAATCTCTCCACAAAATTATTATTATTACTTTTTTCTTTTTCCAATCTTTTTATTTATTATTATTATTATTTGCAATTTCAGTCTTATGTTGTGTTATTGGGATCTCATTCTCATGGGTTAGACGCTACAGAGAAAGATTTTAAAAGGGTGGTTGATTCCCATCACAAATTACTTGGATCCTTCTTGAGGAGGTTTGGAAACTTTATCTAATTAATTATTTCATTGAATTGTTTTTTCAAAATACAATTTGGAATGATTTTAAAAATTTAAAATTATTTCAGTGAAGAAAAGGCAAAAGATGCCATATTTTACTCCTATAAGAAGAATATAAACGGATTTGCAGCAACTCTAGACGATGAAGATGCAACCCGACTTGCAAGTACTATACGCAACTTTTATATATATATATATACATACTGACTATTTATAAAAATATATTTAGAATTTGAATGATATTAATTTATAACAAATTTAAAAAATGGGACAGATCATCCAGAAGTAGCAGCAGTATTGCCAAACAAAGCAAAAAACTTATACACAACACATTCATGGGAATTCATGCATTTGGAGAAAAATGGTGTAATTCCCCCTTCTTCTCCTTGGTGGAGGGCTAAATTTGGAAAAGATGTCATTATTGCCAATCTTGACACAGGTGATTTTTTTTTTTTTCAAATTTTTTTTTTTTAAATTGTGTATCCTAATTTTTGGAATATTTTTGAAATAGGTGTATGGCCAGAGTCAAAGAGTTTTGGCGAACATGGCATAGTTGGACCTGCCCCATCAAAGTGGAAAGGAGGTTGTACTGATGATAAAACCCCTGATGGAGTGCCTTGTAACCAAAAATTAATCGGAGCAAAGTACTTCAACAAGGGCTACTTCGAGTACTTGAAATCTGAAAATTCCACTGTTGACCTCTCATCTATCATCAACTCCACTCGCGACTACAACGGTCATGGATCCCACACACTGTCCACGGCTGGTGGCAACTATGTGGTCGGCGCCAGCGTGTTTGGGTCCGGTATCGGAACAGCTAAAGGAGGATCTCCAAAGGCTCGTGTTGCTGCTTATAAGGTTTGTTGGCCATACGAGCATGGTGGTTGCTTCGATGCTGACATCACTGAGGCATTTGACCATGCCATCCACGATGGTGTTGATGTTCTTTCACTCTCTCTCGGCAGTGATGCCATCAAATACTCCGAGGATGCCATCGCCATTGCCTCCTTCCATGCAGTGAAGAAGGGAATTCCTGTCGTGTGCGCTGTTGGGAACTCTGGTCCCTTACCGAAGACGGCTTCTAATACTGCTCCTTGGATTTTGACTGTTGGTGCAAGTACTTTGGATCGTGAATTTTATGCTCCTGTTGTGCTTCGAAATGGTTATAAATTCATGGTTCGTATCTCTCTCTATATTTAACCAAATTAAGACATTCAACCTGGCTTTATAAATATTAATGTACACACCATAAATACTTTTTCTTTTCATTACCATATTGACATGGTATTAGAATTTTTCGGTAGTTATGGTTCCATTATAGTTGATCCTTTGTTGGTGATTAGGGTTCAAGTCATTCAAAAGGATTAAGGGGAAGGAATCTATACCCATTGATAACCGGAGCTCAGGCAAAAGCTGGTAACGCCACTGAGGACGATGCCATGCTTTGCAAGCCGGAAACATTGGACCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGAAACGGCAAGGTTGGACAAAGGAAAACAAGCGGCCCTTGCCGGTGCTGTTGGAATGATTTTATGCAACGATAAGCTCAGTGGGACTTCCATCAACCCAGATTTTCACGTTCTTCCAGCTTCTCACATCAATTACCATGATGGTCAAGTTCTTCTATCTTACACCAACTCCGCCAGGTAATACATCAAAATTTATACTTTCAGTTTTTATTATTTTGCTATTGTATTGTACGTTTGTTTATGGTCAGATTTTCTTTTGTTGGACAGATATCCAATGGGGTGCTTAATCCCACCATTGGCAAGAGTTAACACCAAACCAGCTCCCACTATGGCGGTCTTCTCATCCCGAGGACCCAATACTATTTCACCAGAGATTATCAAGGTTTTCTTTTCCTAATTCGATGGACCATTTACATTTTCTTTGAAAATATATCTTAAACTATCAAAAATAGCAAACTTATTGTAACTGTATTAAGTCTGCCACTGTATTAAATCAAAAACAAAAGTTTGCCACCATTCAGCCAAAATGTATATTGTAAAATATGGGTTGTGATTGTAAAATGTATATTGTAAAATGTTAAAGTATTATTCACTCTTAAATCAGTGTTATATTTGTTGTGATTTTGAATAAAAAAATTTGCAGCCTGATGTGACCGCTCCAGGAGTAGACATAATTGCGGCATTTTCAGAAGCTATAAGTCCAACTCGCGATCCATCTGATAACAGAACAACTCCATTCATTACAATGTCAGGTACCTCCATGTCTTGCCCCCACGTCGCTGGCCTCGTCGGCCTCCTCCGAAATCTCCACCCTGATTGGACCCCCTCCGCCATTAAATCTGCCATCATGACCTCCGCCCAAGTCCGTGACAACACATTGAACCCCATGCTCGATGGGGGATCTCTTGGCCTCGACCCTGCCACTCCATTTGCATATGGCTCCGGCCACATCAACCCTACTGGAGCCGTTGACCCCGGCCTTGTCTACGACCTCTCCCCCAACGACTACTTGGAATTCCTCTGCGCCAGCGGCTACGACGAGAGGACCATTCGTGCATTCTCTGATGAACCTTTCAAATGTCCTGCTTCTGCTTCTGTTTTGAACCTTAATTACCCTTCCATTGGGGTTCAAAATTTGAAAGATAGTGTCACTATCACTAGAAAGCTGAAGAACGTTGGGACTCCTGGAGTTTATAAAGCTCAGATTCTCCACCCCAACGTAGTTCAGGTTTCTGTGAAGCCCAGATTTCTGAAGTTTGAGAGAGTTGGAGAAGAGAAGAGCTTTGAGTTGACGGTCAGTGGAGTTGTGCCAAAGAATCGTTTTGCTTACGGCGCTTTGATTTGGAGTGATGGCAGACACTTTGTGAGGAGTCCCATTGTTGTTTCTTCGGGCTTATTCTGAATTGCCCGTTTTTATATTTCAAATTAGCGGTACCATATTTTTATATTTCTAGGAATTCATTCTTTATATTCTATTAGAGTTAGTTAAATAGTTAATGAGCTTTTTGTTTTGTACAGTTATAATAACTCTCCTCTGTAATAAAGGTGGCTATATATATATATATATATGTATGTATGTATATGTATATATGTATATGCCTCTTCTTTTATCAATAATAAAGTAAGAGGATTTATTTAGG

mRNA sequence

ATGAAATTCTTTATTTCTCCTCTCATTTTCTTCTCATTTCTTTTGTTAATATCACCTGCCATTGCTACCAAAAAGTCTTATGTTGTGTTATTGGGATCTCATTCTCATGGGTTAGACGCTACAGAGAAAGATTTTAAAAGGGTGGTTGATTCCCATCACAAATTACTTGGATCCTTCTTGAGGAGTGAAGAAAAGGCAAAAGATGCCATATTTTACTCCTATAAGAAGAATATAAACGGATTTGCAGCAACTCTAGACGATGAAGATGCAACCCGACTTGCAAATCATCCAGAAGTAGCAGCAGTATTGCCAAACAAAGCAAAAAACTTATACACAACACATTCATGGGAATTCATGCATTTGGAGAAAAATGGTGTAATTCCCCCTTCTTCTCCTTGGTGGAGGGCTAAATTTGGAAAAGATGTCATTATTGCCAATCTTGACACAGGTGTATGGCCAGAGTCAAAGAGTTTTGGCGAACATGGCATAGTTGGACCTGCCCCATCAAAGTGGAAAGGAGGTTGTACTGATGATAAAACCCCTGATGGAGTGCCTTGTAACCAAAAATTAATCGGAGCAAAGTACTTCAACAAGGGCTACTTCGAGTACTTGAAATCTGAAAATTCCACTGTTGACCTCTCATCTATCATCAACTCCACTCGCGACTACAACGGTCATGGATCCCACACACTGTCCACGGCTGGTGGCAACTATGTGGTCGGCGCCAGCGTGTTTGGGTCCGGTATCGGAACAGCTAAAGGAGGATCTCCAAAGGCTCGTGTTGCTGCTTATAAGGTTTGTTGGCCATACGAGCATGGTGGTTGCTTCGATGCTGACATCACTGAGGCATTTGACCATGCCATCCACGATGGTGTTGATGTTCTTTCACTCTCTCTCGGCAGTGATGCCATCAAATACTCCGAGGATGCCATCGCCATTGCCTCCTTCCATGCAGTGAAGAAGGGAATTCCTGTCGTGTGCGCTGTTGGGAACTCTGGTCCCTTACCGAAGACGGCTTCTAATACTGCTCCTTGGATTTTGACTGTTGGTGCAAGTACTTTGGATCGTGAATTTTATGCTCCTGTTGTGCTTCGAAATGGTTATAAATTCATGGGTTCAAGTCATTCAAAAGGATTAAGGGGAAGGAATCTATACCCATTGATAACCGGAGCTCAGGCAAAAGCTGGTAACGCCACTGAGGACGATGCCATGCTTTGCAAGCCGGAAACATTGGACCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGAAACGGCAAGGTTGGACAAAGGAAAACAAGCGGCCCTTGCCGGTGCTGTTGGAATGATTTTATGCAACGATAAGCTCAGTGGGACTTCCATCAACCCAGATTTTCACGTTCTTCCAGCTTCTCACATCAATTACCATGATGGTCAAGTTCTTCTATCTTACACCAACTCCGCCAGATATCCAATGGGGTGCTTAATCCCACCATTGGCAAGAGTTAACACCAAACCAGCTCCCACTATGGCGGTCTTCTCATCCCGAGGACCCAATACTATTTCACCAGAGATTATCAAGCCTGATGTGACCGCTCCAGGAGTAGACATAATTGCGGCATTTTCAGAAGCTATAAGTCCAACTCGCGATCCATCTGATAACAGAACAACTCCATTCATTACAATGTCAGGTACCTCCATGTCTTGCCCCCACGTCGCTGGCCTCGTCGGCCTCCTCCGAAATCTCCACCCTGATTGGACCCCCTCCGCCATTAAATCTGCCATCATGACCTCCGCCCAAGTCCGTGACAACACATTGAACCCCATGCTCGATGGGGGATCTCTTGGCCTCGACCCTGCCACTCCATTTGCATATGGCTCCGGCCACATCAACCCTACTGGAGCCGTTGACCCCGGCCTTGTCTACGACCTCTCCCCCAACGACTACTTGGAATTCCTCTGCGCCAGCGGCTACGACGAGAGGACCATTCGTGCATTCTCTGATGAACCTTTCAAATGTCCTGCTTCTGCTTCTGTTTTGAACCTTAATTACCCTTCCATTGGGGTTCAAAATTTGAAAGATAGTGTCACTATCACTAGAAAGCTGAAGAACGTTGGGACTCCTGGAGTTTATAAAGCTCAGATTCTCCACCCCAACGTAGTTCAGGTTTCTGTGAAGCCCAGATTTCTGAAGTTTGAGAGAGTTGGAGAAGAGAAGAGCTTTGAGTTGACGGTCAGTGGAGTTGTGCCAAAGAATCGTTTTGCTTACGGCGCTTTGATTTGGAGTGATGGCAGACACTTTGTGAGGAGTCCCATTGTTGTTTCTTCGGGCTTATTCTGA

Coding sequence (CDS)

ATGAAATTCTTTATTTCTCCTCTCATTTTCTTCTCATTTCTTTTGTTAATATCACCTGCCATTGCTACCAAAAAGTCTTATGTTGTGTTATTGGGATCTCATTCTCATGGGTTAGACGCTACAGAGAAAGATTTTAAAAGGGTGGTTGATTCCCATCACAAATTACTTGGATCCTTCTTGAGGAGTGAAGAAAAGGCAAAAGATGCCATATTTTACTCCTATAAGAAGAATATAAACGGATTTGCAGCAACTCTAGACGATGAAGATGCAACCCGACTTGCAAATCATCCAGAAGTAGCAGCAGTATTGCCAAACAAAGCAAAAAACTTATACACAACACATTCATGGGAATTCATGCATTTGGAGAAAAATGGTGTAATTCCCCCTTCTTCTCCTTGGTGGAGGGCTAAATTTGGAAAAGATGTCATTATTGCCAATCTTGACACAGGTGTATGGCCAGAGTCAAAGAGTTTTGGCGAACATGGCATAGTTGGACCTGCCCCATCAAAGTGGAAAGGAGGTTGTACTGATGATAAAACCCCTGATGGAGTGCCTTGTAACCAAAAATTAATCGGAGCAAAGTACTTCAACAAGGGCTACTTCGAGTACTTGAAATCTGAAAATTCCACTGTTGACCTCTCATCTATCATCAACTCCACTCGCGACTACAACGGTCATGGATCCCACACACTGTCCACGGCTGGTGGCAACTATGTGGTCGGCGCCAGCGTGTTTGGGTCCGGTATCGGAACAGCTAAAGGAGGATCTCCAAAGGCTCGTGTTGCTGCTTATAAGGTTTGTTGGCCATACGAGCATGGTGGTTGCTTCGATGCTGACATCACTGAGGCATTTGACCATGCCATCCACGATGGTGTTGATGTTCTTTCACTCTCTCTCGGCAGTGATGCCATCAAATACTCCGAGGATGCCATCGCCATTGCCTCCTTCCATGCAGTGAAGAAGGGAATTCCTGTCGTGTGCGCTGTTGGGAACTCTGGTCCCTTACCGAAGACGGCTTCTAATACTGCTCCTTGGATTTTGACTGTTGGTGCAAGTACTTTGGATCGTGAATTTTATGCTCCTGTTGTGCTTCGAAATGGTTATAAATTCATGGGTTCAAGTCATTCAAAAGGATTAAGGGGAAGGAATCTATACCCATTGATAACCGGAGCTCAGGCAAAAGCTGGTAACGCCACTGAGGACGATGCCATGCTTTGCAAGCCGGAAACATTGGACCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGAAACGGCAAGGTTGGACAAAGGAAAACAAGCGGCCCTTGCCGGTGCTGTTGGAATGATTTTATGCAACGATAAGCTCAGTGGGACTTCCATCAACCCAGATTTTCACGTTCTTCCAGCTTCTCACATCAATTACCATGATGGTCAAGTTCTTCTATCTTACACCAACTCCGCCAGATATCCAATGGGGTGCTTAATCCCACCATTGGCAAGAGTTAACACCAAACCAGCTCCCACTATGGCGGTCTTCTCATCCCGAGGACCCAATACTATTTCACCAGAGATTATCAAGCCTGATGTGACCGCTCCAGGAGTAGACATAATTGCGGCATTTTCAGAAGCTATAAGTCCAACTCGCGATCCATCTGATAACAGAACAACTCCATTCATTACAATGTCAGGTACCTCCATGTCTTGCCCCCACGTCGCTGGCCTCGTCGGCCTCCTCCGAAATCTCCACCCTGATTGGACCCCCTCCGCCATTAAATCTGCCATCATGACCTCCGCCCAAGTCCGTGACAACACATTGAACCCCATGCTCGATGGGGGATCTCTTGGCCTCGACCCTGCCACTCCATTTGCATATGGCTCCGGCCACATCAACCCTACTGGAGCCGTTGACCCCGGCCTTGTCTACGACCTCTCCCCCAACGACTACTTGGAATTCCTCTGCGCCAGCGGCTACGACGAGAGGACCATTCGTGCATTCTCTGATGAACCTTTCAAATGTCCTGCTTCTGCTTCTGTTTTGAACCTTAATTACCCTTCCATTGGGGTTCAAAATTTGAAAGATAGTGTCACTATCACTAGAAAGCTGAAGAACGTTGGGACTCCTGGAGTTTATAAAGCTCAGATTCTCCACCCCAACGTAGTTCAGGTTTCTGTGAAGCCCAGATTTCTGAAGTTTGAGAGAGTTGGAGAAGAGAAGAGCTTTGAGTTGACGGTCAGTGGAGTTGTGCCAAAGAATCGTTTTGCTTACGGCGCTTTGATTTGGAGTGATGGCAGACACTTTGTGAGGAGTCCCATTGTTGTTTCTTCGGGCTTATTCTGA

Protein sequence

MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLGSDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF
BLAST of CsaV3_1G015340 vs. NCBI nr
Match: XP_011648463.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus] >KGN64981.1 hypothetical protein Csa_1G171030 [Cucumis sativus])

HSP 1 Score: 1574.7 bits (4076), Expect = 0.0e+00
Identity = 775/775 (100.00%), Postives = 775/775 (100.00%), Query Frame = 0

Query: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60
           MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL
Sbjct: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60

Query: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120
           RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH
Sbjct: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120

Query: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180
           LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT
Sbjct: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180

Query: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240
           PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV
Sbjct: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240

Query: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300
           GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG
Sbjct: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300

Query: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360
           SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA
Sbjct: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360

Query: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420
           PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI
Sbjct: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420

Query: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480
           LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY
Sbjct: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480

Query: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540
           TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA
Sbjct: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540

Query: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600
           ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN
Sbjct: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600

Query: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660
           TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR
Sbjct: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660

Query: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720
           AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS
Sbjct: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720

Query: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 776
           VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF
Sbjct: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775

BLAST of CsaV3_1G015340 vs. NCBI nr
Match: XP_008464322.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo])

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 595/775 (76.77%), Postives = 665/775 (85.81%), Query Frame = 0

Query: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60
           MK + S LI FS LL+ISPAIA KKSY+VLLGSHSHGL+  ++D +RV DSHHKLLGS +
Sbjct: 6   MKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSII 65

Query: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120
            S+EKA+DAIFYSYK+NINGFAA +D+E+AT+LA HPEVAAVL NKAK L+TTHSWEFMH
Sbjct: 66  GSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMH 125

Query: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180
           LEKNGVIPPSS W RAK GKDVII NLDTGVW ESKSFGEHGIVG  PSKWKGGCT DKT
Sbjct: 126 LEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCT-DKT 185

Query: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240
           PDGV CN+KLIGAKYFNKG+  YL S+N T   +S+INSTRDY+GHGSHTLSTAGG+YV 
Sbjct: 186 PDGVSCNRKLIGAKYFNKGFLAYLNSQNLT---ASVINSTRDYDGHGSHTLSTAGGSYVS 245

Query: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300
           GASVFG G+GTAKGGSPKARVA+YKVCWP E GGCF+ADI EAFDHAIHD VDVLSLSLG
Sbjct: 246 GASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLG 305

Query: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360
            +   Y +D IAIA+FHAVKKGIPVVC+ GNSGP  +T SNTAPWILTVGASTLDREF A
Sbjct: 306 GEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQA 365

Query: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420
           PV L+NG+ +MGSS SKGL+G  LYPLITGA+AKA NAT + AMLCKP+TLDHSKVKGKI
Sbjct: 366 PVELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKI 425

Query: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480
           LVCLRG+TAR+DKG+QAALAGAVGMILCNDKLSG     D HVLPASHINY+DGQ + SY
Sbjct: 426 LVCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSY 485

Query: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540
             S + PMG LIPP A+VNTKPAP+MA FSSRGPN IS           GV+IIAAFSEA
Sbjct: 486 IKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISXXXXXXXXXXXGVNIIAAFSEA 545

Query: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600
           +SPT +P DNRT PFITMSGTSMSCPHV+GLVGLLR LHP W+PSAIKSAIMTSA++RDN
Sbjct: 546 VSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDN 605

Query: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660
           T  PMLDGGS  L PATPFAYGSGHI PTGA+DPGLVYDLSPNDYLEFLCASGY+E+TI+
Sbjct: 606 TKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQ 665

Query: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720
           AFSD PFKCPASAS+LN NYPSIGVQNL  SVT+TRKLKNV TPGVYKA+++HPN V+V 
Sbjct: 666 AFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVL 725

Query: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 776
           VKP+ LKFERVGEEK FEL ++G VP+N+   G LIW+DG+HFVRSPIVVSSGLF
Sbjct: 726 VKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of CsaV3_1G015340 vs. NCBI nr
Match: XP_004139597.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 581/755 (76.95%), Postives = 653/755 (86.49%), Query Frame = 0

Query: 21  IATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSYKKNING 80
           +A KKSY+VLLGSHSHGL+ T++D KRV DSHHKLLGS   S+EKA++AIFYSYKKNING
Sbjct: 1   MAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNING 60

Query: 81  FAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWWRAKFGK 140
           FAA +D+E+A +LA HPEVAAVLPN+AK L+TTHSWEFMHLEKNGVIPPSS W RAK GK
Sbjct: 61  FAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK 120

Query: 141 DVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQKLIGAKYFNKGY 200
           DVIIANLDTGVWPESKSFGEHGIVGP PSKWKGGCT DKT D VPCN+KLIGAKYFNKG+
Sbjct: 121 DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCT-DKTLDRVPCNRKLIGAKYFNKGF 180

Query: 201 FEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSGIGTAKGGSPKAR 260
             YLKSEN T   + +INSTRDY+GHGSHTLSTAGG+YV GASVFG G+GTAKGGSPKAR
Sbjct: 181 LAYLKSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKAR 240

Query: 261 VAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLGSDAIKYSEDAIAIASFHAVK 320
           VAAYKVCWP E GGCFDADI +AFDHAIHD VDVLSLSLG +   Y +D IAI++FHAVK
Sbjct: 241 VAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVK 300

Query: 321 KGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNGYKFMGSSHSKGLR 380
           KGIPVVC+ GNSGP  +T SNTAPWILTVGAST+DREF APV L+NG+++MGSS SKGL+
Sbjct: 301 KGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLK 360

Query: 381 GRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA 440
           G  LYPLITGA+AKA NAT ++A LCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALA
Sbjct: 361 GDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALA 420

Query: 441 GAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYPMGCLIPPLARVNT 500
           GAVGMILCND+LSG     D HVLPASHINY+DGQ + SY  + + PMG LIPP A+VNT
Sbjct: 421 GAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNT 480

Query: 501 KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSG 560
           KPAPTMA FSSRGPN IS           GV+IIAAFSEA+SPT +P DNRT PFITMSG
Sbjct: 481 KPAPTMAAFSSRGPNLISXXXXXXXXXXXGVNIIAAFSEAVSPTGEPFDNRTVPFITMSG 540

Query: 561 TSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFA 620
           TSMSCPHV+GLVGLLR LHP W+PSAIKSAIMTSA++RDNT  PMLDGGS  L P+TPFA
Sbjct: 541 TSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFA 600

Query: 621 YGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNY 680
           YGSGHI PTGA+DPGLVYDLSPNDYLEFLCASGY+E+TI+AFSD PFKCPASAS+LNLNY
Sbjct: 601 YGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNY 660

Query: 681 PSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELT 740
           PSIGVQNL  SVT+TRKLKNV TPGVYK ++ HPN V+V VKP+ LKFERVGEEKSFELT
Sbjct: 661 PSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELT 720

Query: 741 VSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 776
           ++G VP+++   G LIW+DG+HFVRSPIVVSS LF
Sbjct: 721 ITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 751

BLAST of CsaV3_1G015340 vs. NCBI nr
Match: KGN64982.1 (hypothetical protein Csa_1G171040 [Cucumis sativus])

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 578/751 (76.96%), Postives = 650/751 (86.55%), Query Frame = 0

Query: 25  KSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSYKKNINGFAAT 84
           +SY+VLLGSHSHGL+ T++D KRV DSHHKLLGS   S+EKA++AIFYSYKKNINGFAA 
Sbjct: 11  QSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAI 70

Query: 85  LDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWWRAKFGKDVII 144
           +D+E+A +LA HPEVAAVLPN+AK L+TTHSWEFMHLEKNGVIPPSS W RAK GKDVII
Sbjct: 71  MDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVII 130

Query: 145 ANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQKLIGAKYFNKGYFEYL 204
           ANLDTGVWPESKSFGEHGIVGP PSKWKGGCT DKT D VPCN+KLIGAKYFNKG+  YL
Sbjct: 131 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCT-DKTLDRVPCNRKLIGAKYFNKGFLAYL 190

Query: 205 KSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSGIGTAKGGSPKARVAAY 264
           KSEN T   + +INSTRDY+GHGSHTLSTAGG+YV GASVFG G+GTAKGGSPKARVAAY
Sbjct: 191 KSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAY 250

Query: 265 KVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLGSDAIKYSEDAIAIASFHAVKKGIP 324
           KVCWP E GGCFDADI +AFDHAIHD VDVLSLSLG +   Y +D IAI++FHAVKKGIP
Sbjct: 251 KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP 310

Query: 325 VVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNGYKFMGSSHSKGLRGRNL 384
           VVC+ GNSGP  +T SNTAPWILTVGAST+DREF APV L+NG+++MGSS SKGL+G  L
Sbjct: 311 VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL 370

Query: 385 YPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVG 444
           YPLITGA+AKA NAT ++A LCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAGAVG
Sbjct: 371 YPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG 430

Query: 445 MILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYPMGCLIPPLARVNTKPAP 504
           MILCND+LSG     D HVLPASHINY+DGQ + SY  + + PMG LIPP A+VNTKPAP
Sbjct: 431 MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAP 490

Query: 505 TMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMS 564
           TMA FSSRGPN IS           GV+IIAAFSEA+SPT +P DNRT PFITMSGTSMS
Sbjct: 491 TMAAFSSRGPNLISXXXXXXXXXXXGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS 550

Query: 565 CPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSG 624
           CPHV+GLVGLLR LHP W+PSAIKSAIMTSA++RDNT  PMLDGGS  L P+TPFAYGSG
Sbjct: 551 CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG 610

Query: 625 HINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNYPSIG 684
           HI PTGA+DPGLVYDLSPNDYLEFLCASGY+E+TI+AFSD PFKCPASAS+LNLNYPSIG
Sbjct: 611 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIG 670

Query: 685 VQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTVSGV 744
           VQNL  SVT+TRKLKNV TPGVYK ++ HPN V+V VKP+ LKFERVGEEKSFELT++G 
Sbjct: 671 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 730

Query: 745 VPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 776
           VP+++   G LIW+DG+HFVRSPIVVSS LF
Sbjct: 731 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of CsaV3_1G015340 vs. NCBI nr
Match: XP_023520266.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 579/776 (74.61%), Postives = 655/776 (84.41%), Query Frame = 0

Query: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60
           M F    LI F F  LISPAIA KKSY+VLLGSH+HGL+ +  D +R VDSHHKLLGSFL
Sbjct: 1   MNFPTFVLILFGFFWLISPAIAIKKSYIVLLGSHAHGLEVSAVDLQRAVDSHHKLLGSFL 60

Query: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120
            S  KAKDAIFYSYK +INGFAA LD+E+AT+LA HPEVAAVLPNKAK L+TT SWEFMH
Sbjct: 61  GSSGKAKDAIFYSYKNHINGFAAILDEEEATKLAKHPEVAAVLPNKAKELHTTRSWEFMH 120

Query: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180
           LEKNGV PPSSPW +AK G++VIIANLDTGVWPESKSFGEHGIVG  PS+WKGGCT DKT
Sbjct: 121 LEKNGVSPPSSPWRKAKSGRNVIIANLDTGVWPESKSFGEHGIVGGVPSRWKGGCT-DKT 180

Query: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240
           PD VPCN+KLIGAKYFN G   YLKSEN T +LS I+NSTRDY GHGSHTLSTAGG+YV 
Sbjct: 181 PDAVPCNRKLIGAKYFNHGVIAYLKSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVS 240

Query: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWP-YEHGGCFDADITEAFDHAIHDGVDVLSLSL 300
           G SVFGSGIGTAKGGSPKA VAAYKVCWP ++ GGCFDADI +AFDHAIHDGVDVLSLSL
Sbjct: 241 GVSVFGSGIGTAKGGSPKAHVAAYKVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSLSL 300

Query: 301 GSDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFY 360
           GS   +Y +D IAIASFHA+KKGIPVVC+ GN GP   T SNTAPWILTVGASTLDREF 
Sbjct: 301 GSPPAEYYDDIIAIASFHALKKGIPVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQ 360

Query: 361 APVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGK 420
           AP+ LRNG  F GSS S  L GR LYPLITGAQAKA  A+ DDAMLCKP+TLDHSKVKGK
Sbjct: 361 APIELRNGKHFKGSSLSGPLSGRKLYPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGK 420

Query: 421 ILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLS 480
           ILVCLRG+++R+DKG QA LAGAVGMILCND+LSG  I  D HVLPASHI+Y+DGQV+ S
Sbjct: 421 ILVCLRGDSSRVDKGVQALLAGAVGMILCNDRLSGFEIIADPHVLPASHISYNDGQVVSS 480

Query: 481 YTNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSE 540
           Y NS + PMG LIPPL++VNTKP+PTMA FSSRGPN +SPEIIKPDVTAPGV+IIAAFS 
Sbjct: 481 YINSTKNPMGHLIPPLSKVNTKPSPTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSG 540

Query: 541 AISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRD 600
           A+SPT +P DNRT P+ITMSGTSMSCPHV+G+VGLL+ LHP W+P+AIKSAIMTSA++RD
Sbjct: 541 AVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPKWSPAAIKSAIMTSARIRD 600

Query: 601 NTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTI 660
           NT+N MLDGGS    PATPF +GSGHI PTGA+DPGLVYDLSPNDYLEFLCASGY E+ +
Sbjct: 601 NTMNIMLDGGSPIFAPATPFIFGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNL 660

Query: 661 RAFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQV 720
           R F+D  FKCP S+S+LN NYPSIGVQNL  +VT+TR+LKNVG PGVY+ ++  P  V+V
Sbjct: 661 RVFADGNFKCPISSSILNFNYPSIGVQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKV 720

Query: 721 SVKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 776
           SVKP  LKF+++GEEK FELT+ G +  ++  YG LIW+DG+HFVRSPIVVSSG F
Sbjct: 721 SVKPSVLKFKKIGEEKRFELTMIGAMANSQIGYGTLIWTDGKHFVRSPIVVSSGFF 775

BLAST of CsaV3_1G015340 vs. TAIR10
Match: AT5G59810.1 (Subtilase family protein)

HSP 1 Score: 854.4 bits (2206), Expect = 5.6e-248
Identity = 433/759 (57.05%), Postives = 535/759 (70.49%), Query Frame = 0

Query: 15  LLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSY 74
           L  SPA A KKSY+V LGSH+H    +      V  SH   L SF+ S E AK+AIFYSY
Sbjct: 30  LFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSY 89

Query: 75  KKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWW 134
           K++INGFAA LD+ +A  +A HP+V +V PNK + L+TTHSW FM L KNGV+  SS W 
Sbjct: 90  KRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWN 149

Query: 135 RAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQKLIGAK 194
           +A +G+D IIANLDTGVWPESKSF + G  G  P++WKG C  D     VPCN+KLIGA+
Sbjct: 150 KAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD-----VPCNRKLIGAR 209

Query: 195 YFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSGIGTAKG 254
           YFNKGY  Y     + +  ++   + RD++GHGSHTLSTA GN+V GA+VFG G GTA G
Sbjct: 210 YFNKGYLAY-----TGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 269

Query: 255 GSPKARVAAYKVCWPYEHGG-CFDADITEAFDHAIHDGVDVLSLSLGSDAIKYSEDAIAI 314
           GSPKARVAAYKVCWP   G  CFDADI  A + AI DGVDVLS S+G DA  Y  D IAI
Sbjct: 270 GSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAI 329

Query: 315 ASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNGYKFMGS 374
            SFHAVK G+ VVC+ GNSGP   T SN APW++TVGAS++DREF A V L+NG  F G+
Sbjct: 330 GSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGT 389

Query: 375 SHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGETARLDK 434
           S SK L    +Y LI+ A A   N    DA+LCK  +LD  KVKGKILVCLRG+ AR+DK
Sbjct: 390 SLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDK 449

Query: 435 GKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYPMGCLIP 494
           G QAA AGA GM+LCNDK SG  I  D HVLPAS I+Y DG+ L SY +S + P G +  
Sbjct: 450 GMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKA 509

Query: 495 PLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTT 554
           P A +NTKPAP MA FSSRGPNTI+P I+KPD+TAPGV+IIAAF+EA  PT   SDNR T
Sbjct: 510 PTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRT 569

Query: 555 PFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGL 614
           PF T SGTSMSCPH++G+VGLL+ LHP W+P+AI+SAIMT+++ R+N   PM+D      
Sbjct: 570 PFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE---SF 629

Query: 615 DPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEP-FKCPAS 674
             A PF+YGSGH+ P  A  PGLVYDL+  DYL+FLCA GY+   ++ F+++P + C   
Sbjct: 630 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 689

Query: 675 ASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVG 734
           A++L+ NYPSI V NL  S+T+TRKLKNVG P  Y A+   P  V+VSV+P+ L F + G
Sbjct: 690 ANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTG 749

Query: 735 EEKSFELTVSGV-VPKNRFAYGALIWSDGRHFVRSPIVV 771
           E K F++T+  + V  + + +G L W+D  H+VRSPIVV
Sbjct: 750 EVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774

BLAST of CsaV3_1G015340 vs. TAIR10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 747.3 bits (1928), Expect = 9.6e-216
Identity = 392/776 (50.52%), Postives = 517/776 (66.62%), Query Frame = 0

Query: 11  FSFLLLI-------SPAIATK--KSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLR 70
           FSFLLL+          +A+K   SYVV  G+HSH  + TE    RV ++H+  LGSF  
Sbjct: 7   FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66

Query: 71  SEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHL 130
           S E+A DAIFYSY K+INGFAA LD + A  ++ HPEV +V PNKA  L+TT SW+F+ L
Sbjct: 67  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126

Query: 131 EKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTP 190
           E N  +P SS W +A+FG+D IIANLDTGVWPESKSF + G+ GP PS+WKG C + K  
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDA 186

Query: 191 DGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVG 250
               CN+KLIGA+YFNKGY   +   NS+ D      S RD +GHGSHTLSTA G++V G
Sbjct: 187 T-FHCNRKLIGARYFNKGYAAAVGHLNSSFD------SPRDLDGHGSHTLSTAAGDFVPG 246

Query: 251 ASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGG-CFDADITEAFDHAIHDGVDVLSLSLG 310
            S+FG G GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHDG DV+S+SLG
Sbjct: 247 VSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLG 306

Query: 311 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 370
            +   +  D++AI SFHA KK I VVC+ GNSGP   T SN APW +TVGAST+DREF +
Sbjct: 307 GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFAS 366

Query: 371 PVVLRNGYKFMGSS-HSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGK 430
            +VL NG  + G S  S  L     YP++    AKA NA+  DA LCK  +LD  K KGK
Sbjct: 367 NLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGK 426

Query: 431 ILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLS 490
           ILVCLRG+  R++KG+  AL G +GM+L N  ++G  +  D HVLPA+ +   D   +  
Sbjct: 427 ILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSR 486

Query: 491 YTNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSE 550
           Y +  + P+  + P    +  KPAP MA FSS+GP+ ++P+I+KPD+TAPGV +IAA++ 
Sbjct: 487 YISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTG 546

Query: 551 AISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRD 610
           A+SPT +  D R   F  +SGTSMSCPH++G+ GLL+  +P W+P+AI+SAIMT+A + D
Sbjct: 547 AVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMD 606

Query: 611 NTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTI 670
           +   P+ +  ++    ATPF++G+GH+ P  AV+PGLVYDL   DYL FLC+ GY+   I
Sbjct: 607 DIPGPIQNATNM---KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQI 666

Query: 671 RAFSDEPFKCPA-SASVLNLNYPSI-GVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVV 730
             FS   F C +   S++NLNYPSI         VT++R +KNVG P +Y  ++ +P  V
Sbjct: 667 SVFSGNNFTCSSPKISLVNLNYPSITXXXXXXXXVTVSRTVKNVGRPSMYTVKVNNPQGV 726

Query: 731 QVSVKPRFLKFERVGEEKSFELTV---SGVVPKNRFAYGALIWSDGRHFVRSPIVV 771
            V+VKP  L F +VGE+K+F++ +    G V K  + +G L+WSD +H VRSPIVV
Sbjct: 727 YVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG-YVFGELVWSDKKHRVRSPIVV 770

BLAST of CsaV3_1G015340 vs. TAIR10
Match: AT5G67360.1 (Subtilase family protein)

HSP 1 Score: 545.0 bits (1403), Expect = 7.3e-155
Identity = 324/781 (41.49%), Postives = 449/781 (57.49%), Query Frame = 0

Query: 4   FISPLIFFSFLLL----ISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSF 63
           F+S   FF  L L    +S + + + +Y+V +          +       D H     S 
Sbjct: 5   FLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHM---------AKSQMPSSFDLHSNWYDSS 64

Query: 64  LRSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFM 123
           LRS   + + + Y+Y+  I+GF+  L  E+A  L   P V +VLP     L+TT +  F+
Sbjct: 65  LRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFL 124

Query: 124 HLEKN--GVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTD 183
            L+++   + P +  +       DV++  LDTGVWPESKS+ + G  GP PS WKGGC  
Sbjct: 125 GLDEHTADLFPEAGSY------SDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEA 184

Query: 184 DKTPDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGN 243
                   CN+KLIGA++F +GY    +S    +D S    S RD +GHG+HT STA G+
Sbjct: 185 GTNFTASLCNRKLIGARFFARGY----ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGS 244

Query: 244 YVVGASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSL 303
            V GAS+ G   GTA+G +P+ARVA YKVCW    GGCF +DI  A D AI D V+VLS+
Sbjct: 245 VVEGASLLGYASGTARGMAPRARVAVYKVCW---LGGCFSSDILAAIDKAIADNVNVLSM 304

Query: 304 SLGSDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDRE 363
           SLG     Y  D +AI +F A+++GI V C+ GN+GP   + SN APWI TVGA TLDR+
Sbjct: 305 SLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRD 364

Query: 364 FYAPVVLRNGYKFMGSSHSKG-LRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKV 423
           F A  +L NG  F G S  KG      L P I      A NAT  +  LC   TL   KV
Sbjct: 365 FPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA--GNASNATNGN--LCMTGTLIPEKV 424

Query: 424 KGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQV 483
           KGKI++C RG  AR+ KG     AG VGMIL N   +G  +  D H+LPA+ +    G +
Sbjct: 425 KGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDI 484

Query: 484 LLSYTNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAA 543
           +  Y  +   P   +      V  KP+P +A FSSRGPN+I+P I+KPD+ APGV+I+AA
Sbjct: 485 IRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAA 544

Query: 544 FSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQ 603
           ++ A  PT   SD+R   F  +SGTSMSCPHV+GL  LL+++HP+W+P+AI+SA+MT+A 
Sbjct: 545 WTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAY 604

Query: 604 VRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDE 663
                  P+LD  +    P+TPF +G+GH++PT A +PGL+YDL+  DYL FLCA  Y  
Sbjct: 605 KTYKDGKPLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTS 664

Query: 664 RTIRAFSDEPFKCPASA--SVLNLNYPSIGVQ-NLKDSVTITRKLKNVGTPGVYKAQILH 723
             IR+ S   + C  S   SV +LNYPS  V  +   +   TR + +VG  G Y  ++  
Sbjct: 665 PQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTS 724

Query: 724 PNV-VQVSVKPRFLKFERVGEEKSFEL--TVSGVVPKNRFAYGALIWSDGRHFVRSPIVV 772
               V++SV+P  L F+   E+KS+ +  TV    P    ++G++ WSDG+H V SP+ +
Sbjct: 725 ETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 755

BLAST of CsaV3_1G015340 vs. TAIR10
Match: AT3G14240.1 (Subtilase family protein)

HSP 1 Score: 532.3 bits (1370), Expect = 4.9e-151
Identity = 307/749 (40.99%), Postives = 436/749 (58.21%), Query Frame = 0

Query: 48  VVDSHHKLLGSFLRSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKA 107
           +  +H     S L S   +  +I ++Y    +GF+A L  +DA++L +HP V +V+P + 
Sbjct: 40  IFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQV 99

Query: 108 KNLYTTHSWEFMHL---EKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIV 167
           ++L+TT S EF+ L   +K G++  S       FG D++I  +DTGVWPE  SF + G+ 
Sbjct: 100 RHLHTTRSPEFLGLRSTDKAGLLEESD------FGSDLVIGVIDTGVWPERPSFDDRGL- 159

Query: 168 GPAPSKWKGGCTDDKTPDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYN 227
           GP P KWKG C   +      CN+KL+GA++F  GY    ++ N  ++ ++   S RD +
Sbjct: 160 GPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGY----EATNGKMNETTEFRSPRDSD 219

Query: 228 GHGSHTLSTAGGNYVVGASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAF 287
           GHG+HT S + G YV  AS  G   G A G +PKAR+AAYKVCW   + GC+D+DI  AF
Sbjct: 220 GHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW---NSGCYDSDILAAF 279

Query: 288 DHAIHDGVDVLSLSLGSDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAP 347
           D A+ DGVDV+SLS+G   + Y  DAIAI +F A+ +GI V  + GN GP   T +N AP
Sbjct: 280 DTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAP 339

Query: 348 WILTVGASTLDREFYAPVVLRNGYKFMGSS--HSKGLRGRNLYPLITGAQAKAGNATEDD 407
           W+ TVGA T+DR+F A V L NG    G S     GL    +YPL+ G     G+     
Sbjct: 340 WMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGY--S 399

Query: 408 AMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFH 467
           + LC   +LD + VKGKI++C RG  +R  KG+     G +GMI+ N    G  +  D H
Sbjct: 400 SSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCH 459

Query: 468 VLPASHINYHDGQVLLSY------TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNT 527
           VLPA+ +    G  +  Y      + S+++P   ++    R+  +PAP +A FS+RGPN 
Sbjct: 460 VLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNP 519

Query: 528 ISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLR 587
            +PEI+KPDV APG++I+AA+ + I P+   SDNR T F  +SGTSM+CPHV+GL  LL+
Sbjct: 520 ETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLK 579

Query: 588 NLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGL 647
             HPDW+P+AI+SA++T+A   DN+  PM+D  S G + ++   YGSGH++PT A+DPGL
Sbjct: 580 AAHPDWSPAAIRSALITTAYTVDNSGEPMMD-ESTG-NTSSVMDYGSGHVHPTKAMDPGL 639

Query: 648 VYDLSPNDYLEFLCASGYDERTIRAFSDEPFKCPA---SASVLNLNYPSIGV-----QNL 707
           VYD++  DY+ FLC S Y    I   +     C     +  V NLNYPS  V        
Sbjct: 640 VYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGES 699

Query: 708 KDSVTITRKLKNVG-TPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTVSGVVPK 767
           K S    R + NVG +  VY+ +I  P    V+V+P  L F RVG++ SF + V     K
Sbjct: 700 KMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVK 759

Query: 768 -----NRFAYGALIWSDGRHFVRSPIVVS 772
                     G ++WSDG+  V SP+VV+
Sbjct: 760 LSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of CsaV3_1G015340 vs. TAIR10
Match: AT4G34980.1 (subtilisin-like serine protease 2)

HSP 1 Score: 521.9 bits (1343), Expect = 6.6e-148
Identity = 314/784 (40.05%), Postives = 452/784 (57.65%), Query Frame = 0

Query: 8   LIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAK 67
           L+F SF  +   A    K+++  +   S            +  +H+    +    E +  
Sbjct: 9   LLFLSFPFISFAASQAAKTFIFRIDGGS---------MPSIFPTHYHWYSTEFAEESR-- 68

Query: 68  DAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVI 127
             I + Y    +GF+A +  ++A  L NHP V AV  ++ + L+TT S +F+ L+     
Sbjct: 69  --IVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ--- 128

Query: 128 PPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCN 187
                W  + +G DVII   DTG+WPE +SF +  + GP P +W+G C          CN
Sbjct: 129 --KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGARFSPRNCN 188

Query: 188 QKLIGAKYFNKG-YFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFG 247
           +K+IGA++F KG     +   N TV+      S RD +GHG+HT STA G +   AS+ G
Sbjct: 189 RKIIGARFFAKGQQAAVIGGINKTVEFL----SPRDADGHGTHTSSTAAGRHAFKASMSG 248

Query: 248 SGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSL-GSDAI- 307
              G AKG +PKAR+AAYKVCW  +  GC D+DI  AFD A+ DGVDV+S+S+ G D I 
Sbjct: 249 YASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGIT 308

Query: 308 -KYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVV 367
             Y  D IAI S+ A  KGI V  + GN GP   + +N APW+ TVGAST+DR F A  +
Sbjct: 309 SPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAI 368

Query: 368 LRNGYKFMGSSHSKG--LRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKIL 427
           L +G++  G S   G  L GR ++P++       G +    A LC   TLD  +V+GKI+
Sbjct: 369 LGDGHRLRGVSLYAGVPLNGR-MFPVV-----YPGKSGMSSASLCMENTLDPKQVRGKIV 428

Query: 428 VCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYT 487
           +C RG + R+ KG     AG VGMIL N   +G  +  D H++PA  +  ++G  + +Y 
Sbjct: 429 ICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYA 488

Query: 488 NSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAI 547
           +S   P+  +      V  KPAP +A FS RGPN +SPEI+KPD+ APGV+I+AA+++A+
Sbjct: 489 SSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAV 548

Query: 548 SPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNT 607
            PT  PSD R T F  +SGTSM+CPHV+G   LL++ HPDW+P+ I+SA+MT+  + DN+
Sbjct: 549 GPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNS 608

Query: 608 LNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRA 667
              ++D  S G   ATP+ YGSGH+N   A++PGLVYD++ +DY+ FLC+ GY  +TI+ 
Sbjct: 609 NRSLID-ESTG-KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQV 668

Query: 668 FSDEPFKCPAS--ASVLNLNYPSIGV---QNLKD--SVTITRKLKNVG-TPGVYKAQILH 727
            +  P +CP +   S  NLNYPSI      N +   S T+ R   NVG    VY+A+I  
Sbjct: 669 ITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIES 728

Query: 728 PNVVQVSVKPRFLKFERVGEEKSFELTV-----SGVVPKNRFAYGALIWSD-GRHFVRSP 772
           P  V V+VKP  L F    + +S+ +TV     + V+ +    +G++ W D G+H VRSP
Sbjct: 729 PRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSP 759

BLAST of CsaV3_1G015340 vs. Swiss-Prot
Match: sp|F4JXC5|SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 1.0e-246
Identity = 433/759 (57.05%), Postives = 535/759 (70.49%), Query Frame = 0

Query: 15  LLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSY 74
           L  SPA A KKSY+V LGSH+H    +      V  SH   L SF+ S E AK+AIFYSY
Sbjct: 30  LFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSY 89

Query: 75  KKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWW 134
           K++INGFAA LD+ +A  +A HP+V +V PNK + L+TTHSW FM L KNGV+  SS W 
Sbjct: 90  KRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWN 149

Query: 135 RAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQKLIGAK 194
           +A +G+D IIANLDTGVWPESKSF + G  G  P++WKG C  D     VPCN+KLIGA+
Sbjct: 150 KAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD-----VPCNRKLIGAR 209

Query: 195 YFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSGIGTAKG 254
           YFNKGY  Y     + +  ++   + RD++GHGSHTLSTA GN+V GA+VFG G GTA G
Sbjct: 210 YFNKGYLAY-----TGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 269

Query: 255 GSPKARVAAYKVCWPYEHGG-CFDADITEAFDHAIHDGVDVLSLSLGSDAIKYSEDAIAI 314
           GSPKARVAAYKVCWP   G  CFDADI  A + AI DGVDVLS S+G DA  Y  D IAI
Sbjct: 270 GSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAI 329

Query: 315 ASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNGYKFMGS 374
            SFHAVK G+ VVC+ GNSGP   T SN APW++TVGAS++DREF A V L+NG  F G+
Sbjct: 330 GSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGT 389

Query: 375 SHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGETARLDK 434
           S SK L    +Y LI+ A A   N    DA+LCK  +LD  KVKGKILVCLRG+ AR+DK
Sbjct: 390 SLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDK 449

Query: 435 GKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYPMGCLIP 494
           G QAA AGA GM+LCNDK SG  I  D HVLPAS I+Y DG+ L SY +S + P G +  
Sbjct: 450 GMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKA 509

Query: 495 PLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTT 554
           P A +NTKPAP MA FSSRGPNTI+P I+KPD+TAPGV+IIAAF+EA  PT   SDNR T
Sbjct: 510 PTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRT 569

Query: 555 PFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGL 614
           PF T SGTSMSCPH++G+VGLL+ LHP W+P+AI+SAIMT+++ R+N   PM+D      
Sbjct: 570 PFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE---SF 629

Query: 615 DPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEP-FKCPAS 674
             A PF+YGSGH+ P  A  PGLVYDL+  DYL+FLCA GY+   ++ F+++P + C   
Sbjct: 630 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 689

Query: 675 ASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVG 734
           A++L+ NYPSI V NL  S+T+TRKLKNVG P  Y A+   P  V+VSV+P+ L F + G
Sbjct: 690 ANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTG 749

Query: 735 EEKSFELTVSGV-VPKNRFAYGALIWSDGRHFVRSPIVV 771
           E K F++T+  + V  + + +G L W+D  H+VRSPIVV
Sbjct: 750 EVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774

BLAST of CsaV3_1G015340 vs. Swiss-Prot
Match: sp|Q9ZSP5|AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.7e-214
Identity = 392/776 (50.52%), Postives = 517/776 (66.62%), Query Frame = 0

Query: 11  FSFLLLI-------SPAIATK--KSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLR 70
           FSFLLL+          +A+K   SYVV  G+HSH  + TE    RV ++H+  LGSF  
Sbjct: 7   FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66

Query: 71  SEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHL 130
           S E+A DAIFYSY K+INGFAA LD + A  ++ HPEV +V PNKA  L+TT SW+F+ L
Sbjct: 67  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126

Query: 131 EKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTP 190
           E N  +P SS W +A+FG+D IIANLDTGVWPESKSF + G+ GP PS+WKG C + K  
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDA 186

Query: 191 DGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVG 250
               CN+KLIGA+YFNKGY   +   NS+ D      S RD +GHGSHTLSTA G++V G
Sbjct: 187 T-FHCNRKLIGARYFNKGYAAAVGHLNSSFD------SPRDLDGHGSHTLSTAAGDFVPG 246

Query: 251 ASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGG-CFDADITEAFDHAIHDGVDVLSLSLG 310
            S+FG G GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHDG DV+S+SLG
Sbjct: 247 VSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLG 306

Query: 311 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 370
            +   +  D++AI SFHA KK I VVC+ GNSGP   T SN APW +TVGAST+DREF +
Sbjct: 307 GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFAS 366

Query: 371 PVVLRNGYKFMGSS-HSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGK 430
            +VL NG  + G S  S  L     YP++    AKA NA+  DA LCK  +LD  K KGK
Sbjct: 367 NLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGK 426

Query: 431 ILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLS 490
           ILVCLRG+  R++KG+  AL G +GM+L N  ++G  +  D HVLPA+ +   D   +  
Sbjct: 427 ILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSR 486

Query: 491 YTNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSE 550
           Y +  + P+  + P    +  KPAP MA FSS+GP+ ++P+I+KPD+TAPGV +IAA++ 
Sbjct: 487 YISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTG 546

Query: 551 AISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRD 610
           A+SPT +  D R   F  +SGTSMSCPH++G+ GLL+  +P W+P+AI+SAIMT+A + D
Sbjct: 547 AVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMD 606

Query: 611 NTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTI 670
           +   P+ +  ++    ATPF++G+GH+ P  AV+PGLVYDL   DYL FLC+ GY+   I
Sbjct: 607 DIPGPIQNATNM---KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQI 666

Query: 671 RAFSDEPFKCPA-SASVLNLNYPSI-GVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVV 730
             FS   F C +   S++NLNYPSI         VT++R +KNVG P +Y  ++ +P  V
Sbjct: 667 SVFSGNNFTCSSPKISLVNLNYPSITXXXXXXXXVTVSRTVKNVGRPSMYTVKVNNPQGV 726

Query: 731 QVSVKPRFLKFERVGEEKSFELTV---SGVVPKNRFAYGALIWSDGRHFVRSPIVV 771
            V+VKP  L F +VGE+K+F++ +    G V K  + +G L+WSD +H VRSPIVV
Sbjct: 727 YVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG-YVFGELVWSDKKHRVRSPIVV 770

BLAST of CsaV3_1G015340 vs. Swiss-Prot
Match: sp|I1N462|SBT1_SOYBN (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 647.5 bits (1669), Expect = 1.9e-184
Identity = 364/786 (46.31%), Postives = 484/786 (61.58%), Query Frame = 0

Query: 10  FFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDA 69
           FF F  L++    +KK Y+V +G+HSHG   T  D +   DSH+ LLGS   S EKAK+A
Sbjct: 15  FFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEA 74

Query: 70  IFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPP 129
           I YSY ++INGFAA L++E+A  +A +P V +V  +K   L+TT SWEF+ L + G    
Sbjct: 75  IIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---Q 134

Query: 130 SSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGG-CTDDKTPDGV--PC 189
           +S W + +FG++ II N+DTGVWPES+SF + G  G  PSKW+GG C  +K P  +   C
Sbjct: 135 NSAWQKGRFGENTIIGNIDTGVWPESQSFSDKG-YGTVPSKWRGGLCQINKLPGSMKNTC 194

Query: 190 NQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFG 249
           N+KLIGA+Y+NK +      E     L  ++++ RD+ GHG+HTLSTAGGN+V GA VF 
Sbjct: 195 NRKLIGARYYNKAF------EAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFA 254

Query: 250 SGIGTAKGGSPKARVAAYKVCWPY-EHGGCFDADITEAFDHAIHDGVDVLSLSLGSDAIK 309
            G GTAKGGSP+ARVAAYKVCW   +   C+ AD+  A D AI DGVDV+++S G   + 
Sbjct: 255 VGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVV 314

Query: 310 YSE----DAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAP 369
            +E    D I+I +FHA+ K I +V + GN GP P T +N APW+ T+ ASTLDR+F + 
Sbjct: 315 TAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSN 374

Query: 370 VVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKIL 429
           + + N     G+S    L     + LI    AK  NAT  DA LC+  TLD +KV GKI+
Sbjct: 375 LTI-NNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIV 434

Query: 430 VCLR-GETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVL-----PASHINYHDGQ 489
           +C R G+   + +G +A  AGA GMIL N   +G +++ + HV      P          
Sbjct: 435 LCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHG 494

Query: 490 VLLSYTNSARYPM--GCLIPPLARVNT----KPAPTMAVFSSRGPNTISPEIIKPDVTAP 549
           V  +       P+  G  I  ++R  T    KPAP MA FSSRGPN I P I+KPDVTAP
Sbjct: 495 VKTTAIGDEDDPLKTGDTI-KMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAP 554

Query: 550 GVDIIAAFSEAISPTRDPSDNRT-TPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIK 609
           GV+I+AA+SE  S +    DNR    F  + GTSMSCPH +G+ GLL+  HP W+P+AIK
Sbjct: 555 GVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIK 614

Query: 610 SAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEF 669
           SAIMT+A   DNT  P+ D     L  A  FAYGSGH+ P  A++PGLVYDLS  DYL F
Sbjct: 615 SAIMTTATTLDNTNRPIQDAFDKTL--ADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNF 674

Query: 670 LCASGYDERTIRAFS-DEPFKCPASASVLNLNYPSIGVQNLK-DSVTITRKLKNVGTPGV 729
           LCASGYD++ I A + +  F C  S SV +LNYPSI + NL+   VTI R + NVG P  
Sbjct: 675 LCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPST 734

Query: 730 YKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTV--SGVVPKNRFAYGALIWSDGRHFV 771
           Y      PN   ++V P  L F ++GE K+F++ V  S    + ++ +G L W+DG+H V
Sbjct: 735 YTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIV 786

BLAST of CsaV3_1G015340 vs. Swiss-Prot
Match: sp|O65351|SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 1.3e-153
Identity = 324/781 (41.49%), Postives = 449/781 (57.49%), Query Frame = 0

Query: 4   FISPLIFFSFLLL----ISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSF 63
           F+S   FF  L L    +S + + + +Y+V +          +       D H     S 
Sbjct: 5   FLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHM---------AKSQMPSSFDLHSNWYDSS 64

Query: 64  LRSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFM 123
           LRS   + + + Y+Y+  I+GF+  L  E+A  L   P V +VLP     L+TT +  F+
Sbjct: 65  LRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFL 124

Query: 124 HLEKN--GVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTD 183
            L+++   + P +  +       DV++  LDTGVWPESKS+ + G  GP PS WKGGC  
Sbjct: 125 GLDEHTADLFPEAGSY------SDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEA 184

Query: 184 DKTPDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGN 243
                   CN+KLIGA++F +GY    +S    +D S    S RD +GHG+HT STA G+
Sbjct: 185 GTNFTASLCNRKLIGARFFARGY----ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGS 244

Query: 244 YVVGASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSL 303
            V GAS+ G   GTA+G +P+ARVA YKVCW    GGCF +DI  A D AI D V+VLS+
Sbjct: 245 VVEGASLLGYASGTARGMAPRARVAVYKVCW---LGGCFSSDILAAIDKAIADNVNVLSM 304

Query: 304 SLGSDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDRE 363
           SLG     Y  D +AI +F A+++GI V C+ GN+GP   + SN APWI TVGA TLDR+
Sbjct: 305 SLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRD 364

Query: 364 FYAPVVLRNGYKFMGSSHSKG-LRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKV 423
           F A  +L NG  F G S  KG      L P I      A NAT  +  LC   TL   KV
Sbjct: 365 FPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA--GNASNATNGN--LCMTGTLIPEKV 424

Query: 424 KGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQV 483
           KGKI++C RG  AR+ KG     AG VGMIL N   +G  +  D H+LPA+ +    G +
Sbjct: 425 KGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDI 484

Query: 484 LLSYTNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAA 543
           +  Y  +   P   +      V  KP+P +A FSSRGPN+I+P I+KPD+ APGV+I+AA
Sbjct: 485 IRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAA 544

Query: 544 FSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQ 603
           ++ A  PT   SD+R   F  +SGTSMSCPHV+GL  LL+++HP+W+P+AI+SA+MT+A 
Sbjct: 545 WTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAY 604

Query: 604 VRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDE 663
                  P+LD  +    P+TPF +G+GH++PT A +PGL+YDL+  DYL FLCA  Y  
Sbjct: 605 KTYKDGKPLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTS 664

Query: 664 RTIRAFSDEPFKCPASA--SVLNLNYPSIGVQ-NLKDSVTITRKLKNVGTPGVYKAQILH 723
             IR+ S   + C  S   SV +LNYPS  V  +   +   TR + +VG  G Y  ++  
Sbjct: 665 PQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTS 724

Query: 724 PNV-VQVSVKPRFLKFERVGEEKSFEL--TVSGVVPKNRFAYGALIWSDGRHFVRSPIVV 772
               V++SV+P  L F+   E+KS+ +  TV    P    ++G++ WSDG+H V SP+ +
Sbjct: 725 ETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 755

BLAST of CsaV3_1G015340 vs. Swiss-Prot
Match: sp|Q9LUM3|SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 8.8e-150
Identity = 307/749 (40.99%), Postives = 436/749 (58.21%), Query Frame = 0

Query: 48  VVDSHHKLLGSFLRSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKA 107
           +  +H     S L S   +  +I ++Y    +GF+A L  +DA++L +HP V +V+P + 
Sbjct: 40  IFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQV 99

Query: 108 KNLYTTHSWEFMHL---EKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIV 167
           ++L+TT S EF+ L   +K G++  S       FG D++I  +DTGVWPE  SF + G+ 
Sbjct: 100 RHLHTTRSPEFLGLRSTDKAGLLEESD------FGSDLVIGVIDTGVWPERPSFDDRGL- 159

Query: 168 GPAPSKWKGGCTDDKTPDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYN 227
           GP P KWKG C   +      CN+KL+GA++F  GY    ++ N  ++ ++   S RD +
Sbjct: 160 GPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGY----EATNGKMNETTEFRSPRDSD 219

Query: 228 GHGSHTLSTAGGNYVVGASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAF 287
           GHG+HT S + G YV  AS  G   G A G +PKAR+AAYKVCW   + GC+D+DI  AF
Sbjct: 220 GHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW---NSGCYDSDILAAF 279

Query: 288 DHAIHDGVDVLSLSLGSDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAP 347
           D A+ DGVDV+SLS+G   + Y  DAIAI +F A+ +GI V  + GN GP   T +N AP
Sbjct: 280 DTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAP 339

Query: 348 WILTVGASTLDREFYAPVVLRNGYKFMGSS--HSKGLRGRNLYPLITGAQAKAGNATEDD 407
           W+ TVGA T+DR+F A V L NG    G S     GL    +YPL+ G     G+     
Sbjct: 340 WMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGY--S 399

Query: 408 AMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFH 467
           + LC   +LD + VKGKI++C RG  +R  KG+     G +GMI+ N    G  +  D H
Sbjct: 400 SSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCH 459

Query: 468 VLPASHINYHDGQVLLSY------TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNT 527
           VLPA+ +    G  +  Y      + S+++P   ++    R+  +PAP +A FS+RGPN 
Sbjct: 460 VLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNP 519

Query: 528 ISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLR 587
            +PEI+KPDV APG++I+AA+ + I P+   SDNR T F  +SGTSM+CPHV+GL  LL+
Sbjct: 520 ETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLK 579

Query: 588 NLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGL 647
             HPDW+P+AI+SA++T+A   DN+  PM+D  S G + ++   YGSGH++PT A+DPGL
Sbjct: 580 AAHPDWSPAAIRSALITTAYTVDNSGEPMMD-ESTG-NTSSVMDYGSGHVHPTKAMDPGL 639

Query: 648 VYDLSPNDYLEFLCASGYDERTIRAFSDEPFKCPA---SASVLNLNYPSIGV-----QNL 707
           VYD++  DY+ FLC S Y    I   +     C     +  V NLNYPS  V        
Sbjct: 640 VYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGES 699

Query: 708 KDSVTITRKLKNVG-TPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTVSGVVPK 767
           K S    R + NVG +  VY+ +I  P    V+V+P  L F RVG++ SF + V     K
Sbjct: 700 KMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVK 759

Query: 768 -----NRFAYGALIWSDGRHFVRSPIVVS 772
                     G ++WSDG+  V SP+VV+
Sbjct: 760 LSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of CsaV3_1G015340 vs. TrEMBL
Match: tr|A0A0A0LYF1|A0A0A0LYF1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=4 SV=1)

HSP 1 Score: 1574.7 bits (4076), Expect = 0.0e+00
Identity = 775/775 (100.00%), Postives = 775/775 (100.00%), Query Frame = 0

Query: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60
           MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL
Sbjct: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60

Query: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120
           RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH
Sbjct: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120

Query: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180
           LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT
Sbjct: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180

Query: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240
           PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV
Sbjct: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240

Query: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300
           GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG
Sbjct: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300

Query: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360
           SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA
Sbjct: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360

Query: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420
           PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI
Sbjct: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420

Query: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480
           LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY
Sbjct: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480

Query: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540
           TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA
Sbjct: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540

Query: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600
           ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN
Sbjct: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600

Query: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660
           TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR
Sbjct: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660

Query: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720
           AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS
Sbjct: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720

Query: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 776
           VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF
Sbjct: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775

BLAST of CsaV3_1G015340 vs. TrEMBL
Match: tr|A0A1S3CLB7|A0A1S3CLB7_CUCME (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=4 SV=1)

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 595/775 (76.77%), Postives = 665/775 (85.81%), Query Frame = 0

Query: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60
           MK + S LI FS LL+ISPAIA KKSY+VLLGSHSHGL+  ++D +RV DSHHKLLGS +
Sbjct: 6   MKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSII 65

Query: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120
            S+EKA+DAIFYSYK+NINGFAA +D+E+AT+LA HPEVAAVL NKAK L+TTHSWEFMH
Sbjct: 66  GSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMH 125

Query: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180
           LEKNGVIPPSS W RAK GKDVII NLDTGVW ESKSFGEHGIVG  PSKWKGGCT DKT
Sbjct: 126 LEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCT-DKT 185

Query: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240
           PDGV CN+KLIGAKYFNKG+  YL S+N T   +S+INSTRDY+GHGSHTLSTAGG+YV 
Sbjct: 186 PDGVSCNRKLIGAKYFNKGFLAYLNSQNLT---ASVINSTRDYDGHGSHTLSTAGGSYVS 245

Query: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300
           GASVFG G+GTAKGGSPKARVA+YKVCWP E GGCF+ADI EAFDHAIHD VDVLSLSLG
Sbjct: 246 GASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLG 305

Query: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360
            +   Y +D IAIA+FHAVKKGIPVVC+ GNSGP  +T SNTAPWILTVGASTLDREF A
Sbjct: 306 GEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQA 365

Query: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420
           PV L+NG+ +MGSS SKGL+G  LYPLITGA+AKA NAT + AMLCKP+TLDHSKVKGKI
Sbjct: 366 PVELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKI 425

Query: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480
           LVCLRG+TAR+DKG+QAALAGAVGMILCNDKLSG     D HVLPASHINY+DGQ + SY
Sbjct: 426 LVCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSY 485

Query: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540
             S + PMG LIPP A+VNTKPAP+MA FSSRGPN IS           GV+IIAAFSEA
Sbjct: 486 IKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISXXXXXXXXXXXGVNIIAAFSEA 545

Query: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600
           +SPT +P DNRT PFITMSGTSMSCPHV+GLVGLLR LHP W+PSAIKSAIMTSA++RDN
Sbjct: 546 VSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDN 605

Query: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660
           T  PMLDGGS  L PATPFAYGSGHI PTGA+DPGLVYDLSPNDYLEFLCASGY+E+TI+
Sbjct: 606 TKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQ 665

Query: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720
           AFSD PFKCPASAS+LN NYPSIGVQNL  SVT+TRKLKNV TPGVYKA+++HPN V+V 
Sbjct: 666 AFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVL 725

Query: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 776
           VKP+ LKFERVGEEK FEL ++G VP+N+   G LIW+DG+HFVRSPIVVSSGLF
Sbjct: 726 VKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of CsaV3_1G015340 vs. TrEMBL
Match: tr|A0A0A0LVY8|A0A0A0LVY8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=4 SV=1)

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 578/751 (76.96%), Postives = 650/751 (86.55%), Query Frame = 0

Query: 25  KSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSYKKNINGFAAT 84
           +SY+VLLGSHSHGL+ T++D KRV DSHHKLLGS   S+EKA++AIFYSYKKNINGFAA 
Sbjct: 11  QSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAI 70

Query: 85  LDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWWRAKFGKDVII 144
           +D+E+A +LA HPEVAAVLPN+AK L+TTHSWEFMHLEKNGVIPPSS W RAK GKDVII
Sbjct: 71  MDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVII 130

Query: 145 ANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQKLIGAKYFNKGYFEYL 204
           ANLDTGVWPESKSFGEHGIVGP PSKWKGGCT DKT D VPCN+KLIGAKYFNKG+  YL
Sbjct: 131 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCT-DKTLDRVPCNRKLIGAKYFNKGFLAYL 190

Query: 205 KSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSGIGTAKGGSPKARVAAY 264
           KSEN T   + +INSTRDY+GHGSHTLSTAGG+YV GASVFG G+GTAKGGSPKARVAAY
Sbjct: 191 KSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAY 250

Query: 265 KVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLGSDAIKYSEDAIAIASFHAVKKGIP 324
           KVCWP E GGCFDADI +AFDHAIHD VDVLSLSLG +   Y +D IAI++FHAVKKGIP
Sbjct: 251 KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP 310

Query: 325 VVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNGYKFMGSSHSKGLRGRNL 384
           VVC+ GNSGP  +T SNTAPWILTVGAST+DREF APV L+NG+++MGSS SKGL+G  L
Sbjct: 311 VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL 370

Query: 385 YPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVG 444
           YPLITGA+AKA NAT ++A LCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAGAVG
Sbjct: 371 YPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG 430

Query: 445 MILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYPMGCLIPPLARVNTKPAP 504
           MILCND+LSG     D HVLPASHINY+DGQ + SY  + + PMG LIPP A+VNTKPAP
Sbjct: 431 MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAP 490

Query: 505 TMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMS 564
           TMA FSSRGPN IS           GV+IIAAFSEA+SPT +P DNRT PFITMSGTSMS
Sbjct: 491 TMAAFSSRGPNLISXXXXXXXXXXXGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS 550

Query: 565 CPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSG 624
           CPHV+GLVGLLR LHP W+PSAIKSAIMTSA++RDNT  PMLDGGS  L P+TPFAYGSG
Sbjct: 551 CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG 610

Query: 625 HINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNYPSIG 684
           HI PTGA+DPGLVYDLSPNDYLEFLCASGY+E+TI+AFSD PFKCPASAS+LNLNYPSIG
Sbjct: 611 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIG 670

Query: 685 VQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTVSGV 744
           VQNL  SVT+TRKLKNV TPGVYK ++ HPN V+V VKP+ LKFERVGEEKSFELT++G 
Sbjct: 671 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 730

Query: 745 VPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 776
           VP+++   G LIW+DG+HFVRSPIVVSS LF
Sbjct: 731 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of CsaV3_1G015340 vs. TrEMBL
Match: tr|A0A251PDK1|A0A251PDK1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_4G001800 PE=4 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 5.3e-269
Identity = 475/768 (61.85%), Postives = 576/768 (75.00%), Query Frame = 0

Query: 9   IFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKD 68
           + F   L   PA A KKSYVV LGSH+HG   +E D  RV DSH + LGSFL S +KAK+
Sbjct: 12  LMFILSLFQVPAFAIKKSYVVYLGSHAHGPQVSEADLHRVTDSHSEFLGSFLGSTQKAKE 71

Query: 69  AIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIP 128
           AI YSYK++INGFAA L+DE+A  +A HP+V +V  N+ + L+TTHSW+FM LEK+GVI 
Sbjct: 72  AIIYSYKRHINGFAAILEDEEAAEIAKHPKVVSVFLNQGRQLHTTHSWDFMLLEKDGVIH 131

Query: 129 PSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQ 188
           P+S W RA+FG+D II NLDTGVW ES+SF + GI GP P+KWKG C +D T  G PCN+
Sbjct: 132 PTSLWKRARFGEDTIIGNLDTGVWAESESFSDEGI-GPIPAKWKGICQNDTT--GFPCNR 191

Query: 189 KLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSG 248
           KLIGA+YFNKGY  Y     +   L S  NS RD+ GHGSHTLSTA GN+V GA+VFG G
Sbjct: 192 KLIGARYFNKGYASY-----AGAPLRSSFNSARDHEGHGSHTLSTAAGNFVAGANVFGLG 251

Query: 249 IGTAKGGSPKARVAAYKVCWPYEHGG-CFDADITEAFDHAIHDGVDVLSLSLGSDAIKYS 308
            GTAKGGSPKARVAAYKVCWP  +G  CFDADI  AFD AIHDGVDVLS+SLG D   Y 
Sbjct: 252 NGTAKGGSPKARVAAYKVCWPPINGSECFDADIMAAFDAAIHDGVDVLSVSLGGDPSNYL 311

Query: 309 EDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNG 368
           +D ++I +FHAVK GI VVC+ GNSGP   T SN APW++TVGASTLDREF A V LRNG
Sbjct: 312 DDGLSIGAFHAVKNGIVVVCSAGNSGPAAGTVSNVAPWMITVGASTLDREFQAIVQLRNG 371

Query: 369 YKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGE 428
            +  G+S SK L     YPLITGAQAKA NA+  DAMLC   TLD  KVKGKIL CLRG+
Sbjct: 372 LRLKGTSLSKPLPEDRFYPLITGAQAKAANASAHDAMLCIGGTLDPQKVKGKILACLRGD 431

Query: 429 TARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYP 488
           TAR+DKG+QAALAGAVGMILCNDK SG  I  D HVLPAS INY DG  ++SY NS   P
Sbjct: 432 TARIDKGEQAALAGAVGMILCNDKASGNEIIADPHVLPASQINYTDGIAVVSYINSTIDP 491

Query: 489 MGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDP 548
            G + PP A++N KPAP MA FSS+GPNTI+PEI+KPD+TAPGV+IIAA+++A SPT + 
Sbjct: 492 QGFITPPTAQLNAKPAPFMASFSSQGPNTITPEILKPDITAPGVNIIAAYTQATSPTNES 551

Query: 549 SDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLD 608
            D R   F T SGTSMSCPHV+G+VGLL+ L+PDW+PSAI+SAIMT+A+ RDNT NPM +
Sbjct: 552 FDKRRIAFNTESGTSMSCPHVSGVVGLLKTLYPDWSPSAIRSAIMTTARTRDNTANPMKN 611

Query: 609 GGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEP- 668
              +    ATPF+YG+GHI P  A+DPGL+YDL+ NDYL+FLCA GY++  ++ FS+ P 
Sbjct: 612 ASFI---EATPFSYGAGHIRPNRAMDPGLIYDLTVNDYLDFLCAIGYNKTMMQLFSESPN 671

Query: 669 FKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFL 728
           +KCP SAS+L+ NYPSI V  L  SVT+TR++KNVG+PG Y  +   P  V V+V+P  L
Sbjct: 672 YKCPKSASLLDFNYPSIVVPELSGSVTVTRRVKNVGSPGTYAVRAHKPLGVSVTVEPNIL 731

Query: 729 KFERVGEEKSFELTV--SGVVPKNRFAYGALIWSDGRHFVRSPIVVSS 773
           KF+ +GEEKSF++T+   G+     + +G LIWSDG+H+VRSPIVVSS
Sbjct: 732 KFKNIGEEKSFKVTLKAKGLGVTKDYVFGGLIWSDGKHYVRSPIVVSS 768

BLAST of CsaV3_1G015340 vs. TrEMBL
Match: tr|A0A2K1ZUJ9|A0A2K1ZUJ9_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G001600v3 PE=4 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 1.7e-267
Identity = 471/771 (61.09%), Postives = 572/771 (74.19%), Query Frame = 0

Query: 8   LIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAK 67
           L+   F L  SPA A KKSYVV LGSH+HG   ++ D   V DSH + L S+L S EKA+
Sbjct: 11  LLIILFSLFQSPAFAIKKSYVVYLGSHAHGPQISKVDLDAVADSHQEFLASYLGSSEKAR 70

Query: 68  DAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVI 127
           DAI YSY ++INGFAA L++E+A  +A HP V +V  N+ + L+TTHSW+FM LEK+GV+
Sbjct: 71  DAIIYSYDRHINGFAAMLEEEEAAEIARHPNVVSVFLNQGRKLHTTHSWDFMLLEKDGVV 130

Query: 128 PPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCN 187
            PSS W RA+FG+D IIANLDTGVWPES SF E GI GP PSKWKG C +D T  GVPCN
Sbjct: 131 DPSSLWKRARFGEDSIIANLDTGVWPESLSFSEEGI-GPVPSKWKGTCEND-TAVGVPCN 190

Query: 188 QKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGS 247
           +KLIGA+YFN+GY  Y         L+S  NS RD +GHG+HTLSTAGGN+V GA+VFG 
Sbjct: 191 RKLIGARYFNRGYIAYAGG------LTSSDNSARDKDGHGTHTLSTAGGNFVPGANVFGL 250

Query: 248 GIGTAKGGSPKARVAAYKVCWPYEHGG-CFDADITEAFDHAIHDGVDVLSLSLGSDAIKY 307
           G GTAKGGSPKARVA+YKVCWP  +G  CFDADI +AFD AIHDGVDVLS+SLG +   Y
Sbjct: 251 GNGTAKGGSPKARVASYKVCWPPVNGSECFDADIMKAFDMAIHDGVDVLSVSLGGEPTDY 310

Query: 308 SEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRN 367
             D +AI +FHAVK GI VVC+ GNSGP+  T +N APWI+TVGASTLDREF   V LRN
Sbjct: 311 FNDGLAIGAFHAVKNGISVVCSAGNSGPMDGTVTNNAPWIITVGASTLDREFETFVELRN 370

Query: 368 GYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRG 427
           G +  G+S S  L  +  YPLITG QAKA NA+  DA+LCKP++LDH K KGK++VCLRG
Sbjct: 371 GKRLQGTSLSSPLPEKKFYPLITGEQAKAANASAADALLCKPKSLDHEKAKGKVVVCLRG 430

Query: 428 ETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARY 487
           ET R+DKG QAAL GA GMILCNDK SG  I  D HVLPA+ I Y DG  + +Y NS  +
Sbjct: 431 ETGRMDKGYQAALVGAAGMILCNDKASGNEIIADPHVLPAAQITYTDGLAVFAYINSTDH 490

Query: 488 PMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRD 547
            +G +  P A++ TKPAP++A FSSRGPNT++PEI+KPD+TAPGV+IIAAFSEAISPT  
Sbjct: 491 ALGYISAPTAKLGTKPAPSIAAFSSRGPNTVTPEILKPDITAPGVNIIAAFSEAISPTDF 550

Query: 548 PSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPML 607
             D R +PFIT SGTSMSCPHVAG VGLL+ LHPDW+P+AI+SAIMT+A+ R NT+ PM+
Sbjct: 551 DFDKRKSPFITESGTSMSCPHVAGAVGLLKTLHPDWSPAAIRSAIMTTARTRANTMTPMV 610

Query: 608 DGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEP 667
           DG   GL+ ATPF+YGSGHI P  A DPGLVYDLS NDYL+FLCASGY+   I  FSD P
Sbjct: 611 DGRD-GLE-ATPFSYGSGHIRPNRAQDPGLVYDLSINDYLDFLCASGYNSTMIEPFSDGP 670

Query: 668 FKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFL 727
           +KCP S S+ + N PSI ++ L++S+++ RK+KNVG  G Y A +  P  + VSV+P  L
Sbjct: 671 YKCPESTSIFDFNNPSITIRQLRNSMSVIRKVKNVGLTGTYAAHVREPYGILVSVEPSIL 730

Query: 728 KFERVGEEKSFELTV----SGVVPKNRFAYGALIWSDGRHFVRSPIVVSSG 774
            FE  G+EKSF++T      GV   + F  G L W+DGRH+VRSPIVV+ G
Sbjct: 731 TFENKGDEKSFKVTFEAKWDGVTEDHEF--GTLTWTDGRHYVRSPIVVAFG 769

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011648463.10.0e+00100.00PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus] >KGN64981.1 hypothe... [more]
XP_008464322.10.0e+0076.77PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo][more]
XP_004139597.10.0e+0076.95PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus][more]
KGN64982.10.0e+0076.96hypothetical protein Csa_1G171040 [Cucumis sativus][more]
XP_023520266.10.0e+0074.61subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G59810.15.6e-24857.05Subtilase family protein[more]
AT2G04160.19.6e-21650.52Subtilisin-like serine endopeptidase family protein[more]
AT5G67360.17.3e-15541.49Subtilase family protein[more]
AT3G14240.14.9e-15140.99Subtilase family protein[more]
AT4G34980.16.6e-14840.05subtilisin-like serine protease 2[more]
Match NameE-valueIdentityDescription
sp|F4JXC5|SBT54_ARATH1.0e-24657.05Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
sp|Q9ZSP5|AIR3_ARATH1.7e-21450.52Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
sp|I1N462|SBT1_SOYBN1.9e-18446.31Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
sp|O65351|SBT17_ARATH1.3e-15341.49Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
sp|Q9LUM3|SBT15_ARATH8.8e-15040.99Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LYF1|A0A0A0LYF1_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=4 SV=1[more]
tr|A0A1S3CLB7|A0A1S3CLB7_CUCME0.0e+0076.77subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=4 SV=... [more]
tr|A0A0A0LVY8|A0A0A0LVY8_CUCSA0.0e+0076.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=4 SV=1[more]
tr|A0A251PDK1|A0A251PDK1_PRUPE5.3e-26961.85Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_4G001800 PE=4 SV=1[more]
tr|A0A2K1ZUJ9|A0A2K1ZUJ9_POPTR1.7e-26761.09Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G001600v3 PE=... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR034197Peptidases_S8_3
IPR023828Peptidase_S8_Ser-AS
IPR000209Peptidase_S8/S53_dom
IPR036852Peptidase_S8/S53_dom_sf
IPR003137PA_domain
IPR037045S8pro/Inhibitor_I9_sf
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0044699 single-organism process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G015340.1CsaV3_1G015340.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 559..575
score: 54.92
coord: 222..235
score: 36.44
coord: 139..158
score: 32.49
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 26..106
e-value: 2.0E-15
score: 57.1
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3DG3DSA:3.30.70.80coord: 17..111
e-value: 1.1E-20
score: 75.9
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 355..499
e-value: 7.4E-173
score: 577.6
NoneNo IPR availableGENE3DG3DSA:2.60.40.2310coord: 649..772
e-value: 3.6E-34
score: 119.3
NoneNo IPR availablePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 17..771
NoneNo IPR availablePANTHERPTHR10795:SF423SUBTILISIN-LIKE PROTEASE SBT5.3coord: 17..771
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 362..489
e-value: 5.67719E-29
score: 112.122
NoneNo IPR availableSUPERFAMILYSSF54897Protease propeptides/inhibitorscoord: 25..106
NoneNo IPR availableSUPERFAMILYSSF52025PA domaincoord: 395..486
IPR003137PA domainPFAMPF02225PAcoord: 403..477
e-value: 4.8E-10
score: 39.3
IPR036852Peptidase S8/S53 domain superfamilyGENE3DG3DSA:3.40.50.200coord: 141..354
e-value: 7.4E-173
score: 577.6
coord: 500..646
e-value: 7.4E-173
score: 577.6
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILYSSF52743Subtilisin-likecoord: 186..393
coord: 506..636
coord: 112..159
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 139..603
e-value: 3.4E-42
score: 144.6
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 560..570
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 110..595
e-value: 6.12376E-126
score: 380.79

The following gene(s) are paralogous to this gene:

None