CsaV3_1G013320 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G013320 vs. NCBI nr
Match: XP_004147280.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KGN64786.1 hypothetical protein Csa_1G097655 [Cucumis sativus])

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0

Query: 1   MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE
Sbjct: 241 NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE 300

Query: 301 PKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATF 360
           PKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATF
Sbjct: 301 PKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATF 360

Query: 361 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPR 420
           CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPR
Sbjct: 361 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPR 420

Query: 421 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 480
           PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL
Sbjct: 421 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 480

Query: 481 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 540
           FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT
Sbjct: 481 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 540

Query: 541 AIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 595
           AIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK
Sbjct: 541 AIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 594

BLAST of CsaV3_1G013320 vs. NCBI nr
Match: XP_008465591.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo])

HSP 1 Score: 1070.8 bits (2768), Expect = 1.6e-309
Identity = 544/592 (91.89%), Postives = 570/592 (96.28%), Query Frame = 0

Query: 1   MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MV+Q P  SDA H  RPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVSQSPTSSDASHPRRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRL++ERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLKAERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKV+FNHTAY  SATVIC+FLFLPLL+VIREELR+WN  KS  +PIE+PQPKPIDE
Sbjct: 241 NIVQKKVEFNHTAYTLSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENPQPKPIDE 300

Query: 301 PKIITEESKQITEIQKQNLATPPPE-SCFSNICQKPPRGDDYTILQALLSIDMFVLFVAT 360
           PKIITEES QIT+IQ Q+   PPPE +CFSNIC+KPPRGDDYTILQALLSIDMFVLF+AT
Sbjct: 301 PKIITEESNQITKIQNQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIAT 360

Query: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFP 420
           FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAR+KFP
Sbjct: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFP 420

Query: 421 RPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480
           RPLMMTLVLLLSC+GQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST
Sbjct: 421 RPLMMTLVLLLSCIGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480

Query: 481 LFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSI 540
           LFNCGQIASPLGSYILNVKVAGMLYD+EALKQLKEKGLDRSAVKEL C+GK+CYRKSF+I
Sbjct: 481 LFNCGQIASPLGSYILNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTI 540

Query: 541 TAIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTAR 592
           T IVTF GAMVSL+LVMRTREFYKGDIYKKFREEE+KKEE +INGGGE+TA+
Sbjct: 541 TTIVTFAGAMVSLILVMRTREFYKGDIYKKFREEEVKKEEKIINGGGEITAK 592

BLAST of CsaV3_1G013320 vs. NCBI nr
Match: XP_023519125.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 946.8 bits (2446), Expect = 3.5e-272
Identity = 487/586 (83.11%), Postives = 527/586 (89.93%), Query Frame = 0

Query: 9   SDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNL 68
           +DAG     LQF L V+ GRWFTVFATFLIMAGAGATYLFGVYSKQIKS LGYDQTTLNL
Sbjct: 11  ADAGRGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNL 70

Query: 69  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 128
           MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAKPKVWQMCL
Sbjct: 71  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQMCL 130

Query: 129 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 188
           YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIY AVY +DATA
Sbjct: 131 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATA 190

Query: 189 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 248
           LILLI WLPAA+SVVF+F IR+L++ERQPNE RVFYHFLYISIGLA+FIM+MNI+QKKV+
Sbjct: 191 LILLIAWLPAALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVE 250

Query: 249 FNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEES 308
           FNHTAYA SATVIC  LFLPL +VIREEL IWN+K +  +PIE+P    I EPKI  EES
Sbjct: 251 FNHTAYALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENPIEPKIXEPKISIEES 310

Query: 309 KQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLT 368
           K+I          PPP SCFS+IC KP RGDDYTILQALLSIDM VLF+ATFCGLGTSLT
Sbjct: 311 KEIKN-NPDPKPYPPPPSCFSSICSKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLT 370

Query: 369 AVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVL 428
           AVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGR+F+GFVSE+LLA+FKFPRPLMMTLVL
Sbjct: 371 AVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVL 430

Query: 429 LLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 488
           LLSC+GQLLIAFP+PGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS
Sbjct: 431 LLSCIGQLLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 490

Query: 489 PLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGA 548
           PLGSYILNVKVAGM+YD EALKQLKE G+DR+AVKEL C+GK+CYRKSF++ AIVTFVGA
Sbjct: 491 PLGSYILNVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKRCYRKSFTLMAIVTFVGA 550

Query: 549 MVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 595
           +VSLVLVMRTREFY+GDIYK+FR EE+K+E       GE  A ESK
Sbjct: 551 LVSLVLVMRTREFYRGDIYKRFR-EEVKQE----TSAGEAAAVESK 590

BLAST of CsaV3_1G013320 vs. NCBI nr
Match: XP_023519124.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941.4 bits (2432), Expect = 1.5e-270
Identity = 486/586 (82.94%), Postives = 526/586 (89.76%), Query Frame = 0

Query: 9   SDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNL 68
           +DAG     LQF L V+ GRWFTVFATFLIMAGAGATYLFGVYSKQIKS LGYDQTTLNL
Sbjct: 11  ADAGRGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNL 70

Query: 69  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 128
           MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAKPKVWQMCL
Sbjct: 71  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQMCL 130

Query: 129 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 188
           YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIY AVY +DATA
Sbjct: 131 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATA 190

Query: 189 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 248
           LILLI WLPAA+SVVF+F IR+L++ERQPNE RVFYHFLYISIGLA+FIM+MNI+QKKV+
Sbjct: 191 LILLIAWLPAALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVE 250

Query: 249 FNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEES 308
           FNHTAYA SATVIC  LFLPL +VIREEL IWN+K +  +PIE+P      EPKI  EES
Sbjct: 251 FNHTAYALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENP-----IEPKISIEES 310

Query: 309 KQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLT 368
           K+I          PPP SCFS+IC KP RGDDYTILQALLSIDM VLF+ATFCGLGTSLT
Sbjct: 311 KEIKN-NPDPKPYPPPPSCFSSICSKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLT 370

Query: 369 AVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVL 428
           AVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGR+F+GFVSE+LLA+FKFPRPLMMTLVL
Sbjct: 371 AVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVL 430

Query: 429 LLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 488
           LLSC+GQLLIAFP+PGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS
Sbjct: 431 LLSCIGQLLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 490

Query: 489 PLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGA 548
           PLGSYILNVKVAGM+YD EALKQLKE G+DR+AVKEL C+GK+CYRKSF++ AIVTFVGA
Sbjct: 491 PLGSYILNVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKRCYRKSFTLMAIVTFVGA 550

Query: 549 MVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 595
           +VSLVLVMRTREFY+GDIYK+FR EE+K+E       GE  A ESK
Sbjct: 551 LVSLVLVMRTREFYRGDIYKRFR-EEVKQE----TSAGEAAAVESK 585

BLAST of CsaV3_1G013320 vs. NCBI nr
Match: XP_022965019.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 937.6 bits (2422), Expect = 2.1e-269
Identity = 485/585 (82.91%), Postives = 523/585 (89.40%), Query Frame = 0

Query: 10  DAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLM 69
           DA      LQF L V+ GRWFTVFATFLIMAGAGATYLFGVYSKQIKS LGYDQTTLNLM
Sbjct: 12  DAARGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNLM 71

Query: 70  GFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLY 129
           GFFKDLGANVGVLSGLVAEVTPTWFVLLLGS LNFTGYFMIWLAV+GRI KPKVWQMCLY
Sbjct: 72  GFFKDLGANVGVLSGLVAEVTPTWFVLLLGSVLNFTGYFMIWLAVTGRITKPKVWQMCLY 131

Query: 130 ICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATAL 189
           ICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIY AVY +DATAL
Sbjct: 132 ICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATAL 191

Query: 190 ILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQF 249
           ILLI WLPAA+SVVF+F IR+L++ERQPNE RVFYHFLYISIGLA+FIM+MNI+QKKV+F
Sbjct: 192 ILLIAWLPAALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVEF 251

Query: 250 NHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEESK 309
           NHTAYA SATVIC  LFLPL +VIREEL IWN+K +  +PIE+P      EPKI  EESK
Sbjct: 252 NHTAYALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENP-----IEPKISIEESK 311

Query: 310 QITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTA 369
           +I          PPP SCFS+IC KP RGDDYTILQALLSIDM VLF+ATFCGLGTSLTA
Sbjct: 312 EIKN-NPDPKPYPPPPSCFSSICNKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLTA 371

Query: 370 VDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVLL 429
           VDNLGQIGESLGYPLKTV SFVSLVSIWNYFGR+F+GFVSE+LLA+FKFPRPLMMTLVLL
Sbjct: 372 VDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLL 431

Query: 430 LSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASP 489
           LSC+GQLLIAFP+PGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASP
Sbjct: 432 LSCIGQLLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASP 491

Query: 490 LGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAM 549
           LGSYILNVKVAGM+YD EALKQLKE G+DR+AVKEL C+GKQCYRKSF++ AIVTFVGA+
Sbjct: 492 LGSYILNVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKQCYRKSFTLMAIVTFVGAL 551

Query: 550 VSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 595
           VSLVLVMRTREFY+GDIYK+FR EE+K+E  V    GE  A ESK
Sbjct: 552 VSLVLVMRTREFYRGDIYKRFR-EEVKQETSV----GEAAAVESK 585

BLAST of CsaV3_1G013320 vs. TAIR10
Match: AT2G28120.1 (Major facilitator superfamily protein)

HSP 1 Score: 778.5 bits (2009), Expect = 3.0e-225
Identity = 395/564 (70.04%), Postives = 460/564 (81.56%), Query Frame = 0

Query: 18  LQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 77
           ++F +    GRWF VFA+FLIMA AGATYLFG YSK IKS LGYDQTTLNL+GFFKDLGA
Sbjct: 7   VRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGA 66

Query: 78  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQ 137
           NVGVLSGL+AEVTPTWFVL +GSA+NF GYFMIWL V+G++AKPKVWQMCLYIC+GANSQ
Sbjct: 67  NVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQ 126

Query: 138 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLP 197
           NFANTGALVTC++NFPESRG M+GLLKG+ GLSGAI T++Y A+Y  D+ +LILLI WLP
Sbjct: 127 NFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLP 186

Query: 198 AAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASS 257
           AA+S+VFV+ IR  +  RQ NE  VFY FLYISI LA+F+M MNI +K+V F+  AYA+S
Sbjct: 187 AAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAAS 246

Query: 258 ATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQITEIQKQ 317
           AT+ C  LF+PL V +++EL +WN  K   +PIE P    +++PK   E      +  K 
Sbjct: 247 ATICCALLFVPLTVSVKQELEVWNMMK---LPIEEPSEVKVEKPK--KELDLDQDKAAKV 306

Query: 318 NLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVDNLGQIG 377
           N      +SCFS +   PPRG+DYTILQALLS DM +LFVATFCGLG+SLTAVDNLGQIG
Sbjct: 307 NGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIG 366

Query: 378 ESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVLLLSCVGQLL 437
           ESLGYP  TVSSFVSLVSIWNYFGR+F+GFVSE LLA++K PRPLMMTLVLLLSC G LL
Sbjct: 367 ESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLL 426

Query: 438 IAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNV 497
           IAFPVPGSVYIAS+++GFSFGAQLPLLFAIISELFGLKY+STLFNCGQ+ASPLGSYILNV
Sbjct: 427 IAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNV 486

Query: 498 KVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVSLVLVMR 557
           +V GMLYD EALKQL  +GL R  VK+L C+G QCY+  F I A VTF GA+VSL L +R
Sbjct: 487 RVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIR 546

Query: 558 TREFYKGDIYKKFREEEMKKEEMV 582
           TREFYKGDIYKKFRE    + E+V
Sbjct: 547 TREFYKGDIYKKFRESPESESELV 565

BLAST of CsaV3_1G013320 vs. TAIR10
Match: AT2G39210.1 (Major facilitator superfamily protein)

HSP 1 Score: 641.0 bits (1652), Expect = 7.5e-184
Identity = 334/585 (57.09%), Postives = 428/585 (73.16%), Query Frame = 0

Query: 22  LQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGV 81
           +Q++TGRWF  F + LIM+ AGATY+FG+YS  IK  LGYDQTTLNL+ FFKDLGANVGV
Sbjct: 15  IQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGV 74

Query: 82  LSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQNFAN 141
           L+GL+ EVTP WF+LL+G+ LNF GYFMIWLAV+ RI+KP+VW MCLYICVGANSQ+FAN
Sbjct: 75  LAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFAN 134

Query: 142 TGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLPAAIS 201
           TG+LVTC++NFPESRG ++G+LKG+ GLSGAI+T++YRA Y +D   LIL+IGWLPA +S
Sbjct: 135 TGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVS 194

Query: 202 VVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASSATVI 261
             F+ TIR ++ +RQ NE +VFY+FLYIS+GLA F+M++ I+ K   F  +         
Sbjct: 195 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEXXXXXXXX 254

Query: 262 CVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPI--DEPKIIT--------EESKQI 321
                             W  K+   V +  P P  +  ++PK+ +        EESK++
Sbjct: 255 XXXXXXXXXXXXXXXXXXWKEKQ---VALNDPAPINVVTEKPKLDSSEFKDDDGEESKEV 314

Query: 322 TEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVD 381
            E     + TP   SC++ +   P RGDDYTILQAL S+DM +LF+AT CG+G +LTA+D
Sbjct: 315 VE----KVKTP---SCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAID 374

Query: 382 NLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVLLLS 441
           NLGQIG SLGYP ++VS+FVSLVSIWNY+GR+ +G VSE  L ++KFPRPLM+T+VLLLS
Sbjct: 375 NLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLS 434

Query: 442 CVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLG 501
           C G LLIAF VPG +Y+ASVIIGF FGAQ PLLFAIISE+FGLKY+STL+N G +ASP+G
Sbjct: 435 CAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIG 494

Query: 502 SYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVS 561
           SY+LNV+VAG LYD+EA KQ K  G  R   ++L C+G  C++ SF I A VT  G +VS
Sbjct: 495 SYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVS 554

Query: 562 LVLVMRTREFYKGDIYKKFREEEMKKE-EMVINGGGEVT-ARESK 595
           +VLV+RT++FYK DIYKKFRE+ +  E EM        T A+E K
Sbjct: 555 MVLVIRTKKFYKSDIYKKFREKALAAEMEMAAPAAARSTVAKEDK 589

BLAST of CsaV3_1G013320 vs. TAIR10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 315.1 bits (806), Expect = 9.4e-86
Identity = 196/563 (34.81%), Postives = 310/563 (55.06%), Query Frame = 0

Query: 23  QVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGVL 82
           +++  +W  + A+  I   AG +Y FG+YS  +KS   YDQ+TL+ +  FKD+G NVGVL
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 83  SGLVAEVTP--------------TWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 142
           SGLV                    W V+L+G+ LNFTGYF++W +V+G I +P V  MCL
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 143 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 202
           ++ + A S  F NT  +V+ ++NF +  G  +G++KGF GLSGA+L ++Y  V   D   
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 203 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 262
            ILL+  +P+ +SV+ V  + R+      +EK+       +S+ +A ++MI  I++  + 
Sbjct: 184 FILLLAIVPSLLSVL-VMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 243

Query: 263 FNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITE-E 322
               A A +  V+ V L  PLLV +R        + S   P+ S     +D  +  T  E
Sbjct: 244 LPSWANAVTLAVLLVLLSSPLLVAVRAH------RDSIEKPLSSVYSPLVDNLEATTSGE 303

Query: 323 SKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSL 382
              + E +  NL                        LQA+ ++D ++LF+A  CG+G+ +
Sbjct: 304 ILMLDEDKSLNL------------------------LQAMCNVDFWLLFLAMICGMGSGI 363

Query: 383 TAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLV 442
           + ++N+ QIGESL Y    ++S ++L +IWN+ GR   G+VS+ LL R  +PRPL+M   
Sbjct: 364 STINNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATT 423

Query: 443 LLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIA 502
           L    +G L+IA    G++Y  S+I+G  +G+Q  L+  I SELFG+K+  T++N   IA
Sbjct: 424 LGTMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIA 483

Query: 503 SPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKE-LICMGKQCYRKSFSITAIVTFV 562
           SP+GSYI +V++ G +Y             DR+ + E   C G  C+R ++ + A V F+
Sbjct: 484 SPMGSYIFSVRLIGYIY-------------DRTIIGEGNTCYGPHCFRLAYVVIASVAFL 521

Query: 563 GAMVSLVLVMRTREFYKGDIYKK 570
           G +VS VLV RT+  Y+  I++K
Sbjct: 544 GFLVSCVLVFRTKTIYR-QIFEK 521

BLAST of CsaV3_1G013320 vs. TAIR10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 313.5 bits (802), Expect = 2.7e-85
Identity = 192/566 (33.92%), Postives = 309/566 (54.59%), Query Frame = 0

Query: 22  LQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGV 81
           ++++  +W  + A+  I   +GA+Y FG+YS  +KS   YDQ+TL+ +  FKD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 82  LSGLVAEVTPT----------------WFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQ 141
            SGL+     +                W VL +G+   F GYF+IW +V+G I KP V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 142 MCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADD 201
           MCL++ + A SQ F NT  +V+ ++NF +  G  +G++KGF GLSGAIL ++Y  + A D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 202 ATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQK 261
             + ILL+   P  +S++ V  + R+      ++K+       +S+ +A ++MI+ I+  
Sbjct: 181 PASFILLLAVTPTVLSLL-VMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIIL-- 240

Query: 262 KVQFNHTAYASSATVIC--VFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKI 321
           K  F  +++A+  T++C  V L LPLL+  R +      +      +       I  PK 
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLIARRAQ------RDGMEKTVPHDYSPLISSPKA 300

Query: 322 ITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGL 381
            T  ++      K                      ++  +LQA+  +  ++LF+A  CG+
Sbjct: 301 TTSGNQSSEGDSKVEAGL----------------SENLNLLQAMKKLSFWLLFLAMICGM 360

Query: 382 GTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLM 441
           G+ L+ ++N+ QIGESL Y    ++S VSL SIWN+ GR  AG+ S++LL +  +PRPL+
Sbjct: 361 GSGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLL 420

Query: 442 MTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNC 501
           M   L    +G L+IA    G++Y+ SVI+G  +G+Q  L+  I SELFG+++  T+FN 
Sbjct: 421 MAATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNT 480

Query: 502 GQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIV 561
             +ASP+GSYI +V++ G +YD  A            + +   C G  C+R SF I A V
Sbjct: 481 ISVASPIGSYIFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASV 529

Query: 562 TFVGAMVSLVLVMRTREFYKGDIYKK 570
            F G +V++VL  RT+  Y+  + K+
Sbjct: 541 AFFGFLVAIVLFFRTKTLYRQILVKR 529

BLAST of CsaV3_1G013320 vs. TAIR10
Match: AT5G14120.1 (Major facilitator superfamily protein)

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-84
Identity = 197/579 (34.02%), Postives = 307/579 (53.02%), Query Frame = 0

Query: 25  ITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGVLSG 84
           I  RW    A   I + AG  YLFG  S  IKS+L Y+Q  L+ +G  KDLG +VG ++G
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAG 72

Query: 85  LVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQNFANTGA 144
            ++E+ P W  LL+G+  N  GY  +WL V+GR     +W MC+ I VG N + + NTGA
Sbjct: 73  TLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGA 132

Query: 145 LVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLPAAISVVF 204
           LV+ +QNFP+SRG ++G+LKGF GL GAI+++IY  +++ +  +LIL++   PA + V  
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCL 192

Query: 205 VFTIRRLRSERQ--PNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASSATVIC 264
           +F IR +   +Q  P +   F     + + LA ++M + ++Q  V  +H        VI 
Sbjct: 193 MFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSH-------NVIT 252

Query: 265 VFLFL------------------------------PLLVVIREELRIWNTKKSTSVPIES 324
           VF                                 PL+    ++     T       +E 
Sbjct: 253 VFTIXXXXXXXXXXXXXXXXXFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVED 312

Query: 325 PQPKPIDEPKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDM 384
            +PK +D     +E  K+I  +Q Q +      +   N  + P RG+D+T+ QAL+  D 
Sbjct: 313 EKPKDVDLLP-ASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADF 372

Query: 385 FVLFVATFCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESL 444
           +++F +   G G+ LT +DNLGQ+ +SLGY    V   VS++SIWN+ GRI  G+ SE +
Sbjct: 373 WLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELV 432

Query: 445 LARFKFPRPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELF 504
           +  + +PRP+ M +  L+  VG +  A+  PG++YI +++IG  +GA   ++ A  SELF
Sbjct: 433 VRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELF 492

Query: 505 GLKYFSTLFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQC 564
           GLK F  L+N   +A+P GS + +  +A  +YD EA +Q      D      L C G  C
Sbjct: 493 GLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPD--DALRCNGSIC 552

Query: 565 YRKSFSITAIVTFVGAMVSLVLVMRTREFYKGDIYKKFR 572
           +  +  I +    +  M+S++LV RT+  Y   +Y K R
Sbjct: 553 FFLTSLIMSGFCIIACMLSMILVRRTKSVYT-HLYGKTR 578

BLAST of CsaV3_1G013320 vs. Swiss-Prot
Match: sp|F4I9E1|NFD4_ARATH (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.7e-31
Identity = 141/553 (25.50%), Postives = 247/553 (44.67%), Query Frame = 0

Query: 21  PLQVITGRW--FTVF-ATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 80
           PL+ + GRW  +TV  A   I A  G  + F  YS  +KS LG  Q  LN +    DLG 
Sbjct: 33  PLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGK 92

Query: 81  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGAN-S 140
             G  SG+     P   VL   +A+ F GY + WL ++  I  P  + +    C+ A  S
Sbjct: 93  AFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLS 152

Query: 141 QNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWL 200
             + NT   + CI++FP +R   + L   F G+S A+ +  + A+    +   +LL   +
Sbjct: 153 ICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLV 212

Query: 201 PAAISVVF---VFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTA 260
           P  +S      V T   L +    + +R   H   I   LAV      ++      +   
Sbjct: 213 PLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARL 272

Query: 261 YASSATVICVF-LFLPLLVVIREE-LRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQI 320
               A V+ VF L  PLLV  R+  L + N + +     ES     ++  ++  +++   
Sbjct: 273 NFIGAVVLLVFPLCAPLLVYARDYFLPVINARLNH----ESSGYVMLNIDELKNQKTSVS 332

Query: 321 TEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVD 380
           ++   +++ T    +           GD+++    +  ++ ++ ++A FCG    L   +
Sbjct: 333 SKTGYEHMGTAKEGNTVR-------LGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSN 392

Query: 381 NLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVLLLS 440
           NLGQI +SLG   +  ++ V++ S +++FGR+ +    + +  RF+  R     + LL +
Sbjct: 393 NLGQIAQSLG---QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPT 452

Query: 441 CVGQLLIAF--PVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASP 500
            +   L+A       ++  A+ +IG S G       +I S+LFG        N      P
Sbjct: 453 PIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIP 512

Query: 501 LGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAM 560
           +GS +L   +A  +Y+  A   +     D      ++C+G+ CY K+F     ++ +G +
Sbjct: 513 IGS-LLYGYIAASIYEANASPDITPIVSD-----SIVCIGRDCYFKTFVFWGCLSILGVV 562

Query: 561 VSLVLVMRTREFY 563
            SL L +RT+  Y
Sbjct: 573 SSLSLYIRTKPVY 562

BLAST of CsaV3_1G013320 vs. TrEMBL
Match: tr|A0A0A0LXR5|A0A0A0LXR5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G097655 PE=4 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0

Query: 1   MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE
Sbjct: 241 NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE 300

Query: 301 PKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATF 360
           PKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATF
Sbjct: 301 PKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATF 360

Query: 361 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPR 420
           CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPR
Sbjct: 361 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPR 420

Query: 421 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 480
           PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL
Sbjct: 421 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 480

Query: 481 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 540
           FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT
Sbjct: 481 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 540

Query: 541 AIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 595
           AIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK
Sbjct: 541 AIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 594

BLAST of CsaV3_1G013320 vs. TrEMBL
Match: tr|A0A1S3CP80|A0A1S3CP80_CUCME (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103503227 PE=4 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.1e-309
Identity = 544/592 (91.89%), Postives = 570/592 (96.28%), Query Frame = 0

Query: 1   MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MV+Q P  SDA H  RPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVSQSPTSSDASHPRRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRL++ERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLKAERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKV+FNHTAY  SATVIC+FLFLPLL+VIREELR+WN  KS  +PIE+PQPKPIDE
Sbjct: 241 NIVQKKVEFNHTAYTLSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENPQPKPIDE 300

Query: 301 PKIITEESKQITEIQKQNLATPPPE-SCFSNICQKPPRGDDYTILQALLSIDMFVLFVAT 360
           PKIITEES QIT+IQ Q+   PPPE +CFSNIC+KPPRGDDYTILQALLSIDMFVLF+AT
Sbjct: 301 PKIITEESNQITKIQNQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIAT 360

Query: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFP 420
           FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAR+KFP
Sbjct: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFP 420

Query: 421 RPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480
           RPLMMTLVLLLSC+GQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST
Sbjct: 421 RPLMMTLVLLLSCIGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480

Query: 481 LFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSI 540
           LFNCGQIASPLGSYILNVKVAGMLYD+EALKQLKEKGLDRSAVKEL C+GK+CYRKSF+I
Sbjct: 481 LFNCGQIASPLGSYILNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTI 540

Query: 541 TAIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTAR 592
           T IVTF GAMVSL+LVMRTREFYKGDIYKKFREEE+KKEE +INGGGE+TA+
Sbjct: 541 TTIVTFAGAMVSLILVMRTREFYKGDIYKKFREEEVKKEEKIINGGGEITAK 592

BLAST of CsaV3_1G013320 vs. TrEMBL
Match: tr|A0A2P5FTN7|A0A2P5FTN7_9ROSA (Major facilitator OS=Trema orientalis OX=63057 GN=TorMFS10 PE=4 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.0e-235
Identity = 420/571 (73.56%), Postives = 496/571 (86.87%), Query Frame = 0

Query: 19  QFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGAN 78
           +F +Q++ GRWF+VFA+FLIM GAGATYLFGVYSKQIKS+LGYDQ TLNL+GFFKDLGAN
Sbjct: 27  RFLVQLVKGRWFSVFASFLIMFGAGATYLFGVYSKQIKSSLGYDQKTLNLIGFFKDLGAN 86

Query: 79  VGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQN 138
           VGVLSGL+AEVTPTWFVLL+G+A+NF GYFMI+LAV+G+I +PKVWQMCLYIC+GANSQN
Sbjct: 87  VGVLSGLIAEVTPTWFVLLVGAAMNFAGYFMIFLAVAGKIPRPKVWQMCLYICIGANSQN 146

Query: 139 FANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLPA 198
           FANTGALVTC++NFPESRG M+GLLKGF GLSGA+LT+IY A+Y DD+ +LILLI WLPA
Sbjct: 147 FANTGALVTCVKNFPESRGVMLGLLKGFVGLSGAVLTQIYFAIYGDDSKSLILLIAWLPA 206

Query: 199 AISVVFVFTIRRLRSERQ--PNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYAS 258
           A+SVVFV+TIR + ++R   PNE RVFYHFLY+SI LA+FIM+M +VQK+V F+  AYA 
Sbjct: 207 ALSVVFVYTIRTMENDRNRGPNELRVFYHFLYVSIVLALFIMVMTVVQKRVLFSRAAYAG 266

Query: 259 SATVICVFLFLPLLVVIREELRIWNTKKST-SVPIESPQPKPIDEPKIITEESKQITEIQ 318
           SATV+CV LF+PLL+ +REELR+WN KK     P E     P +E K+ +  ++Q    Q
Sbjct: 267 SATVVCVLLFVPLLIAVREELRLWNLKKRVPDPPTEVVFENPKEERKVSSSPAEQ---NQ 326

Query: 319 KQNLATPPPE--SCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVDNL 378
            +NL +P  E  SCF  I   P RG+DYTILQALLS+DM +LF+AT CGLG+SLTAVDNL
Sbjct: 327 IRNLESPKAESKSCFLTIFNPPERGEDYTILQALLSLDMLILFLATLCGLGSSLTAVDNL 386

Query: 379 GQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVLLLSCV 438
           GQIGESLGYP +TVSSFVSLVSIWNYFGR+F+GFVSESLL ++K PRPLMMT VLLLSC+
Sbjct: 387 GQIGESLGYPTRTVSSFVSLVSIWNYFGRVFSGFVSESLLVKYKVPRPLMMTFVLLLSCI 446

Query: 439 GQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSY 498
           G LLIAFP+PGSVYIASVIIGFSFGAQLPLLFAIISELFGLKY+STLFNCGQ+ASPLGSY
Sbjct: 447 GHLLIAFPLPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSY 506

Query: 499 ILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVSLV 558
           ILNVKVAG LYD EALK+L +KGLDRS+VKELIC+GKQCYR SFSI A+VTF GA+VSLV
Sbjct: 507 ILNVKVAGFLYDREALKELAKKGLDRSSVKELICLGKQCYRMSFSILAVVTFFGAVVSLV 566

Query: 559 LVMRTREFYKGDIYKKFR-EEEMKKEEMVIN 584
           LV RTREFY+GDIYKKFR +EE +K+++V++
Sbjct: 567 LVFRTREFYRGDIYKKFRVQEEEEKKDVVVD 594

BLAST of CsaV3_1G013320 vs. TrEMBL
Match: tr|A0A2P5CZK1|A0A2P5CZK1_PARAD (Major facilitator OS=Parasponia andersonii OX=3476 GN=PanMFS10 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 3.0e-235
Identity = 427/591 (72.25%), Postives = 499/591 (84.43%), Query Frame = 0

Query: 2   VAQLPPRSDAGHSCRPL----QFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKS 61
           + Q+PP + AG     L    +F +Q+I GRWF+VFA+FLIM GAGATYLFGVYSKQIKS
Sbjct: 3   MTQIPPGAFAGGGGLKLADMPRFLVQLIKGRWFSVFASFLIMFGAGATYLFGVYSKQIKS 62

Query: 62  ALGYDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGR 121
           +LGYDQ TLNL+GFFKDLGANVGVLSGL+AEVTPTWFVLL+G+A+NF GYFMI+LAV+G+
Sbjct: 63  SLGYDQKTLNLIGFFKDLGANVGVLSGLIAEVTPTWFVLLVGAAMNFAGYFMIFLAVAGK 122

Query: 122 IAKPKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEI 181
           I KPKVWQMCLYIC+GANSQNFANTGALVTC++NFPESRG M+GLLKGF GLSGA+LT+I
Sbjct: 123 IPKPKVWQMCLYICIGANSQNFANTGALVTCVKNFPESRGVMLGLLKGFVGLSGAVLTQI 182

Query: 182 YRAVYADDATALILLIGWLPAAISVVFVFTIRRLRSERQ--PNEKRVFYHFLYISIGLAV 241
           Y A+Y DD+ +LILLI WLPAA+SVVFV+TIR + ++R   PNE +VFYHFLY+SI LA+
Sbjct: 183 YFAIYGDDSKSLILLIAWLPAALSVVFVYTIRTMDNDRNRGPNELQVFYHFLYVSIVLAL 242

Query: 242 FIMIMNIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKST-SVPIESPQ 301
           FIM+M +VQK+V F+  AYA SATV+CV LF+PLL+ IREELR+WN KK     P E   
Sbjct: 243 FIMVMTVVQKRVLFSRAAYAGSATVVCVLLFVPLLIAIREELRLWNLKKRVPDPPTEVVF 302

Query: 302 PKPIDEPKIITEESKQITEIQKQNLATPPPE--SCFSNICQKPPRGDDYTILQALLSIDM 361
             P +E K+ +  ++Q      +NL +P  E  SCF  I   P RG+DYTILQALLS+DM
Sbjct: 303 ENPKEEKKVSSSPAEQNL---IRNLESPKAESKSCFLTIFNPPERGEDYTILQALLSLDM 362

Query: 362 FVLFVATFCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESL 421
            +LF+AT CGLG+SLTAVDNLGQIGESLGYP +TVSSFVSLVSIWNYFGR+F+GFVSESL
Sbjct: 363 LILFLATLCGLGSSLTAVDNLGQIGESLGYPTRTVSSFVSLVSIWNYFGRVFSGFVSESL 422

Query: 422 LARFKFPRPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELF 481
           L ++K PRPLMMT VLLLSC+G LLIAFP+PGSVY ASVIIGFSFGAQLPLLFAIISELF
Sbjct: 423 LVKYKVPRPLMMTFVLLLSCIGHLLIAFPLPGSVYFASVIIGFSFGAQLPLLFAIISELF 482

Query: 482 GLKYFSTLFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQC 541
           GLKY+STLFNCGQ+ASPLGSYILNVKVAG LYD EALK+L +KGLDRS+VKEL C+GKQC
Sbjct: 483 GLKYYSTLFNCGQLASPLGSYILNVKVAGFLYDREALKELAKKGLDRSSVKELTCLGKQC 542

Query: 542 YRKSFSITAIVTFVGAMVSLVLVMRTREFYKGDIYKKFR--EEEMKKEEMV 582
           YR SFSI A+VTF GA+VSLVLV RTREFY+GDIYKKFR  EEE KK+ +V
Sbjct: 543 YRMSFSILAVVTFFGAVVSLVLVFRTREFYRGDIYKKFRVQEEEEKKDAVV 590

BLAST of CsaV3_1G013320 vs. TrEMBL
Match: tr|A0A2G9GHH8|A0A2G9GHH8_9LAMI (Uncharacterized protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_22725 PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 4.4e-231
Identity = 412/567 (72.66%), Postives = 485/567 (85.54%), Query Frame = 0

Query: 18  LQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 77
           L+F   VI GRWF++FA+FLIMAGAGATYLFGVYSK+IK +LGYDQTTLNL+GFFKDLGA
Sbjct: 20  LRFAAHVIQGRWFSLFASFLIMAGAGATYLFGVYSKEIKGSLGYDQTTLNLLGFFKDLGA 79

Query: 78  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQ 137
           NVGVLSGL+AEVTPTWFVLL+GSA+NF GYFMIWLAV+G+IAKPKVWQMC+YIC+GANSQ
Sbjct: 80  NVGVLSGLIAEVTPTWFVLLVGSAMNFAGYFMIWLAVTGKIAKPKVWQMCIYICIGANSQ 139

Query: 138 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLP 197
           NFANTGALVT ++NFPESRG M+GLLKGFTGLSGAILT++Y AVY +DA +LILLI WLP
Sbjct: 140 NFANTGALVTSVKNFPESRGVMLGLLKGFTGLSGAILTQVYLAVYGNDAKSLILLIAWLP 199

Query: 198 AAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASS 257
           AA+SVVFV+TIR ++  RQPNE +VFYHFLY+SI LA+F+M+M IVQK V F+  AYA S
Sbjct: 200 AALSVVFVYTIRAMKVVRQPNELKVFYHFLYVSIVLALFLMVMTIVQKVVTFSQAAYAGS 259

Query: 258 ATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQITEIQKQ 317
            TV+C  LF+PL + IREE  IW+T+K    PI+ PQ    + P+ +  E+   T+ +++
Sbjct: 260 VTVVCALLFVPLFIAIREEFAIWSTQKKQ--PIKVPQITIDNPPQALQLET---TKPEEK 319

Query: 318 NLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVDNLGQIG 377
           +  TPP   CFSNI  KP RG+DYTILQALLS DM +LF+ATFCGLG+SLTAVDNLGQIG
Sbjct: 320 SKKTPP---CFSNIFNKPERGEDYTILQALLSTDMLILFLATFCGLGSSLTAVDNLGQIG 379

Query: 378 ESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVLLLSCVGQLL 437
           ESLGYP KT+ +FVSL+SIWNYFGRIF+GFVSESLLA+ KFPRPLMMTLVL LSC+G LL
Sbjct: 380 ESLGYPTKTIKTFVSLLSIWNYFGRIFSGFVSESLLAKHKFPRPLMMTLVLCLSCIGLLL 439

Query: 438 IAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNV 497
           IAFP  GSVYIASVIIGFSFGAQLPL+FAIISELFGLKY+STLFNCGQ+ASPLGSYILNV
Sbjct: 440 IAFPFQGSVYIASVIIGFSFGAQLPLIFAIISELFGLKYYSTLFNCGQLASPLGSYILNV 499

Query: 498 KVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVSLVLVMR 557
           KVAG LYD EALK+L +KG+ RS+VKEL C+G +CYR +F I A +TF GA+ SL+LV R
Sbjct: 500 KVAGPLYDREALKELAKKGMTRSSVKELTCIGTRCYRSAFIILASITFFGALTSLILVAR 559

Query: 558 TREFYKGDIYKKFREE-EMKKEEMVIN 584
           TREFYKGDIYKKFR+E E  + EM ++
Sbjct: 560 TREFYKGDIYKKFRDEGEEAETEMALS 578