Csa7G450650 (gene) Cucumber (Chinese Long) v2

NameCsa7G450650
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionMidasin; contains IPR027417 (P-loop containing nucleoside triphosphate hydrolase)
LocationChr7 : 18659656 .. 18663549 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATTGATGGTAGTTTCAAACTTGAATCCGCAGTGGAAAGATTTCTTTCCCGTTGCCCATCTCTTACACACCAATCTCCGTTTGAAACGATTATTAGAAAGGTATTTTTGTTTATCACACATGCATGTGATGTTTGATTAGTTTGAGTACAAACATTTTATTTTTCACCTTTTGATATACATTTTTAAAGGGTTTCTCGAATTTTTTAGCGAGACAACAATTTTGATATTGTTACTTTTGAATGCTATTTCAATGAATGGACACAACAAGGCTCTTTGCCTCTGATCGATATGAGAAGTTGCTGTTAAGAATTTGTTTCTCTGCCTCTAATCACATCTTAATATATCACTCCTGTTCTCTGATTTTTGTAGGGATATGGAGTAACTGAAGAGGAAGTTTTGAGTCTTTTGGCTGAGCTGTTTTTGAATCCAAATTATTCAGTATGTTTACTAGGTTGTTTCCGTCCAGTAGCTCAAAAGATCGTGGATAGGGTAATTGGACTGTTAAGGCATGTTCCCAATTTAAGATCAAACTCCACATACTCAGAAATTCATGAAAAGGAGCTGGATGGAGTGGTCAATATAATTTCGTTTTATGATCAGAGAGGGAGGGGGGTTGATCTTCACGAGCTTGCTTGTCTAGCATTCTGTCGTGCCCTTGATTTAGCCCCTTTCTTATTAAGGTTTGTCAGTTACTCTGCTGGATTTTGGATTTGTAGCTTTTAGGGTCTGTCTAGTTTGATGTGTGCGTTCATGTTTTCCATGGTCAATATGCCCTGTAATATATGTTATTTTCTATTTGCACAGGACTATTTTAAATTATTTCAAGTTTGCTCCATGCCCTTTTCACCGTATTGTGGTCAAAGGAGCAAGTTCTGAGGTTTATAACAAGGTATCTATTCTGATTGCGTGCTTGTGCGTTTTATAGAAGATTTATAATGAGCTTATTTGTCTTCATTCTGAATACGATTAGCATATTTCTTAATGAGTTGGTTTTCATGAGAAAAAAAGAAAAAACGAACAGTTCCATCCCTGCCAAGGGCACCTTAAAAACTTGCAGACACTACAGCCAAGGATGTGAGTTTAAGAACAGTAATTTATTGATTTTTTTTAAAAATGAAATGAAACTTTTCATTTATTTAATGAAAAGAGATTAATGCTCAAAATACAAAGTAAAAAATAAGAAGAGAAGGAAATTACCATAGTACATATGAACAAACCAAGGAAAACAAAGACATTCCAAATTAAACACAAATCTTGAATAGAGAATGCTGCAAACGATTTGGAGAAAAAGCACCAAGAGGAGGCTTTAATCTAGGGGAACTCAAACTGATCAAACCGAAGAAGAGACTTTTCTGGCAAAACATGCTGATTCTTTCAAACTAAATCTCCGCAAGAATAACTTTAACTGCATTTAACCATAGGAACTGATATTGAGATTTCAAAAACGGGCCTCCCAATAAATGACGGAGTTTTTAATTCTTCAACAACTTGTAGCATCTCCTTTGTCTTCTTCTGCAATTCAGATTTTATCTGCTTCTATAGGATAATGATTTATTATAATTGACCAGAATTCTTCAAGTTCAAAGAATTATACCTTGAAGCATTTGGAATGAACTTTTGAATATTTCAGGCAAACATGCCCTTTCCACTCTCTCTCTCTCTCTCTCCCTCATTTGAATTTGTAAAGCAAAGGTTCCATTCTCTGGCCTCATGAATTCAGCAGTTGAACATATGCATAATTTTTGGCATGCCATAAAGAAAAATATTTTAAGACCTTATCAGCCATTTGTGCTGCCATAATTTAACCAGATACCCTTGCAAGCCATTTTGAAGAGCGAACACTCCTGGGTGATTTTGTGATTTTCATAATAACAATCCCATCCAGTTAACTAGCAGATAGTCTTTCTCATACTTTCTCGAATTAATATACTAAAGGGGTTCACCTTCTGCAGGGTGTCTTGAATCCTCTTTGCTAATGAAGTCTCATTACCAATGATCAAGGTGCCAGCCTCCGAAGGTGGAGTAACCGTGTTTCCTCTTTCTATTTCCCCTCCAACAGAAACTCTCTAATATTCATTCCCACCGTTTAGCTACTTTGTTAGGGATTTTAAAGATAGAATGGAAATATGCAGGGATGTTACCAACACTGATTGAGCGAGTGTGAACTCTTATGTTGATGGATTTTATTAGAATATTCACTTAAACTTTTGAAGTTGTGTGGATAGAGTGTTATTACGGCTTACTTGGACGGGTTAAAACCAAAGAATTCTACCCAAGAAACAGAAAGACTTCATTTATATTGCACTGTGTAATTGATGTAATCTAAGTAACCTCTAGGAGAAGTCCTCCAAGAACCAAGAACCAAGACTATGGATTTTTTATTAGAATGAATAATATGTTTCATACAAGAGAAGTCTCCTATTTATAGAGTTCTATTACAATTGGGGGTAAAAAGGGAATTAACCTAGAATATGTCCTAATTACCCAATTAACCCTTAGTCTTCTTACATAGTTACATCAGTACTCTTGGTGTTTTTTTTATTAACTTGTAATTTCCTTTTTAAATGGGATTTTCTTTTCTATCCCCCAACCTATAAGGTGCATTCTCATTTTCTTTTTGGCTATAAATCCTGTTTCTTATATATATATTTATATGTATGAACAAGACTTTAATCCAATAATCCATAATTTCTCTGCCTGGCTATTCGTTTTTCTCCAAGAATGTAAGTCATACTAAGTCATACTACTATATCTTTCAAGAACATTAGTCACCTGTTGATTTTTCCTTATCTAGGGTGGATTGCTTGCTGTTCGGTTGTCTTATCGCCTTCTACTGGTGGAGCCTGAAACTTTTACTCAACTATGGGATTGGTCGTGTTTCTTGGACTTTATGAAGCAGCTACCAAACATTGACCTTGATAATGCAAGATTTTTACTTTTACCGGACATAAGATGGTGCGGGATACAGATACTATCAATTTTGCTTAATTTGGGTTATGAAGCAACCACAAATTTGTTGTCTGGGTCTGAAGAAACATTAGCTTGCTTACTGCGGTTTGCTCTTAACCTTGCTGGTTTCTGTTATATAACAAAAATTGCTTCTATATGCTTCATGTTGCCCCTTTCATTTGCAGTAGCTGGGCCCCCTTCCTGTAGTGGGGGTCCCCTGTCTTTTGGGGCTTGGTTTTTTGTGTGCTCGTGTGATCTTTCATTTTTTCTCAATGAAATGCTCTCATTTCAATAATTTTTTTTTTTGCAGATGGGAAGAATTCTGTCAGGATGTATCCATGGAGAAGGCTGGCTCCTATGCTGAGTTATTTGTGAAGGAATCGGTTTCTCAAGGCATGAAAACTGATTTTGACTCAGAAAACTGCCTGCTTGCCAATTCTAGCTTCTGTGGTCCTGCCATTCTCTCGAATCATGATGAGATTGAACGACCAATTAGAAGTCAACGACTTGCTACATGGTAACACTATGACACTTCAATTTAGCCTACTTAGTAAGTTCTGTTTCATTTTTTAAAAAAAGTAAATTGTTTTTTTTTCTCTCTGTTGTCAAGAACAGAAGAAGAGTTTGCATTATTTTGATCCTGTATTTTCTCGTTAGCAATAATTTTGGGATTCCTTTTCTTTGTTTCTTTCTTTATATTTGAGACAATGATTTACTTCACACAGGGGCAGTCCGAAGGCTGAGAATTCATTTTTTATGACATCATCTATAAAGAAAGGTTATGAGATGATTATGTTGGCACTGAGTCAAAAGTGGCCAGTTCTTTTGTATGGTCCCCCTGGAGCTGGGAAATCGGCTCTCATAAATAAGATAGCCAGTGACAGCCATAACCAAGGTAGTAATTGTTTTTATGGTTCAGTAGACATGATCTCTTTATAA

mRNA sequence

ATGGCCATTGATGGTAGTTTCAAACTTGAATCCGCAGTGGAAAGATTTCTTTCCCGTTGCCCATCTCTTACACACCAATCTCCGTTTGAAACGATTATTAGAAAGGGATATGGAGTAACTGAAGAGGAAGTTTTGAGTCTTTTGGCTGAGCTGTTTTTGAATCCAAATTATTCAGTATGTTTACTAGGTTGTTTCCGTCCAGTAGCTCAAAAGATCGTGGATAGGGTAATTGGACTGTTAAGGCATGTTCCCAATTTAAGATCAAACTCCACATACTCAGAAATTCATGAAAAGGAGCTGGATGGAGTGGTCAATATAATTTCGTTTTATGATCAGAGAGGGAGGGGGGTTGATCTTCACGAGCTTGCTTGTCTAGCATTCTGTCGTGCCCTTGATTTAGCCCCTTTCTTATTAAGGACTATTTTAAATTATTTCAAGTTTGCTCCATGCCCTTTTCACCGTATTGTGGTCAAAGGAGCAAGTTCTGAGGTTTATAACAAGGGTGGATTGCTTGCTGTTCGGTTGTCTTATCGCCTTCTACTGGTGGAGCCTGAAACTTTTACTCAACTATGGGATTGGTCGTGTTTCTTGGACTTTATGAAGCAGCTACCAAACATTGACCTTGATAATGCAAGATTTTTACTTTTACCGGACATAAGATGGTGCGGGATACAGATACTATCAATTTTGCTTAATTTGGGTTATGAAGCAACCACAAATTTGTTGTCTGGGTCTGAAGAAACATTAGCTTGCTTACTGCGATGGGAAGAATTCTGTCAGGATGTATCCATGGAGAAGGCTGGCTCCTATGCTGAGTTATTTGTGAAGGAATCGGTTTCTCAAGGCATGAAAACTGATTTTGACTCAGAAAACTGCCTGCTTGCCAATTCTAGCTTCTGTGGTCCTGCCATTCTCTCGAATCATGATGAGATTGAACGACCAATTAGAAGTCAACGACTTGCTACATGGGGCAGTCCGAAGGCTGAGAATTCATTTTTTATGACATCATCTATAAAGAAAGGTTATGAGATGATTATGTTGGCACTGAGTCAAAAGTGGCCAGTTCTTTTGTATGGTCCCCCTGGAGCTGGGAAATCGGCTCTCATAAATAAGATAGCCAGTGACAGCCATAACCAAGGTAGTAATTGTTTTTATGGTTCAGTAGACATGATCTCTTTATAA

Coding sequence (CDS)

ATGGCCATTGATGGTAGTTTCAAACTTGAATCCGCAGTGGAAAGATTTCTTTCCCGTTGCCCATCTCTTACACACCAATCTCCGTTTGAAACGATTATTAGAAAGGGATATGGAGTAACTGAAGAGGAAGTTTTGAGTCTTTTGGCTGAGCTGTTTTTGAATCCAAATTATTCAGTATGTTTACTAGGTTGTTTCCGTCCAGTAGCTCAAAAGATCGTGGATAGGGTAATTGGACTGTTAAGGCATGTTCCCAATTTAAGATCAAACTCCACATACTCAGAAATTCATGAAAAGGAGCTGGATGGAGTGGTCAATATAATTTCGTTTTATGATCAGAGAGGGAGGGGGGTTGATCTTCACGAGCTTGCTTGTCTAGCATTCTGTCGTGCCCTTGATTTAGCCCCTTTCTTATTAAGGACTATTTTAAATTATTTCAAGTTTGCTCCATGCCCTTTTCACCGTATTGTGGTCAAAGGAGCAAGTTCTGAGGTTTATAACAAGGGTGGATTGCTTGCTGTTCGGTTGTCTTATCGCCTTCTACTGGTGGAGCCTGAAACTTTTACTCAACTATGGGATTGGTCGTGTTTCTTGGACTTTATGAAGCAGCTACCAAACATTGACCTTGATAATGCAAGATTTTTACTTTTACCGGACATAAGATGGTGCGGGATACAGATACTATCAATTTTGCTTAATTTGGGTTATGAAGCAACCACAAATTTGTTGTCTGGGTCTGAAGAAACATTAGCTTGCTTACTGCGATGGGAAGAATTCTGTCAGGATGTATCCATGGAGAAGGCTGGCTCCTATGCTGAGTTATTTGTGAAGGAATCGGTTTCTCAAGGCATGAAAACTGATTTTGACTCAGAAAACTGCCTGCTTGCCAATTCTAGCTTCTGTGGTCCTGCCATTCTCTCGAATCATGATGAGATTGAACGACCAATTAGAAGTCAACGACTTGCTACATGGGGCAGTCCGAAGGCTGAGAATTCATTTTTTATGACATCATCTATAAAGAAAGGTTATGAGATGATTATGTTGGCACTGAGTCAAAAGTGGCCAGTTCTTTTGTATGGTCCCCCTGGAGCTGGGAAATCGGCTCTCATAAATAAGATAGCCAGTGACAGCCATAACCAAGGTAGTAATTGTTTTTATGGTTCAGTAGACATGATCTCTTTATAA

Protein sequence

MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVCLLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLLLVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTNLLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFCGPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGPPGAGKSALINKIASDSHNQGSNCFYGSVDMISL*
BLAST of Csa7G450650 vs. Swiss-Prot
Match: MDN1_HUMAN (Midasin OS=Homo sapiens GN=MDN1 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 3.4e-09
Identity = 60/269 (22.30%), Postives = 111/269 (41.26%), Query Frame = 1

Query: 118 DLHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLA----- 177
           DLHE  C++  + +   P +L   L YFK     F R+ ++ + +     G         
Sbjct: 89  DLHERLCVSMSKLIGNHPDVLPFALRYFKDTSPVFQRLFLESSDANPVRYGRRRMKLRDL 148

Query: 178 VRLSYRLLLVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLN 237
           +  +++ L  E   F +LWDWS  +  ++    +            +RW     L+++  
Sbjct: 149 MEAAFKFLQQEQSVFRELWDWSVCVPLLRSHDTL------------VRWYTANCLALVTC 208

Query: 238 LGYEATTNLLS---GSEETLACLLRWEEFCQDVSMEKAGSYAE----LFVKESVSQGMKT 297
           +  E   + L     S+E +   LR  E  Q   +EKA   A     L+ K+   Q ++ 
Sbjct: 209 MNEEHKLSFLKKIFNSDELIHFRLRLLEEAQLQDLEKALVLANPEVSLWRKQKELQYLQG 268

Query: 298 DFDSENCLLANSSFCGPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMI 357
              S +     ++ CG  +L         +   R ++     A  S+ +  S+ K  + +
Sbjct: 269 HLVSSDLSPRVTAVCG-VVLPGQLPAPGELGGNRSSSREQELALRSYVLVESVCKSLQTL 328

Query: 358 MLALSQKWPVLLYGPPGAGKSALINKIAS 375
            +A++ +  VLL GP G GK++L+  +A+
Sbjct: 329 AMAVASQNAVLLEGPIGCGKTSLVEYLAA 344


HSP 2 Score: 32.0 bits (71), Expect = 1.9e+01
Identity = 14/36 (38.89%), Postives = 20/36 (55.56%), Query Frame = 1

Query: 343  EMIMLALSQKWPVLLYGPPGAGKSALINKIASDSHN 379
            + ++ A   K P+LL G PG GK++L+  +A  S N
Sbjct: 1737 QRLLRATKLKKPILLEGSPGVGKTSLVGALAKASGN 1772

BLAST of Csa7G450650 vs. TrEMBL
Match: A0A0A0KAY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G450650 PE=4 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 4.0e-227
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC
Sbjct: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH 120
           LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH
Sbjct: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH 120

Query: 121 ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL 180
           ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL
Sbjct: 121 ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL 180

Query: 181 LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN 240
           LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN
Sbjct: 181 LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN 240

Query: 241 LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC 300
           LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC
Sbjct: 241 LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC 300

Query: 301 GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP 360
           GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP
Sbjct: 301 GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP 360

Query: 361 PGAGKSALINKIASDSHNQGSNCFYGSVDMISL 394
           PGAGKSALINKIASDSHNQGSNCFYGSVDMISL
Sbjct: 361 PGAGKSALINKIASDSHNQGSNCFYGSVDMISL 393

BLAST of Csa7G450650 vs. TrEMBL
Match: A0A0B2NWG0_GLYSO (Midasin OS=Glycine soja GN=glysoja_009586 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 6.3e-103
Identity = 202/390 (51.79%), Postives = 259/390 (66.41%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           MAIDGSF L S++ RFL RCP L    P E++ R G  VTEEEV+++L  +FL+P+Y++ 
Sbjct: 1   MAIDGSFSLHSSLRRFLDRCPKLQCFPPLESLERMGDLVTEEEVVNVLVGVFLHPSYTIP 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRS---NSTYSEIHEKELDGVVNIISFYDQRGRGV 120
           L+GCFRP+AQ IVD+ + LLR VPNL S   ++   +  ++ LDGVVN+I FY Q+GRG+
Sbjct: 61  LIGCFRPIAQHIVDKAVALLRLVPNLMSIPKDTAREDDRDRVLDGVVNVIEFYSQQGRGL 120

Query: 121 DLHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSY 180
           DLHELACLAFCRALD+ PFLL +IL+YF FAP PF R  VK  + E      L A ++SY
Sbjct: 121 DLHELACLAFCRALDMGPFLLSSILSYFNFAPAPFERFSVKQFTIETRE---LHAAQISY 180

Query: 181 RLLLVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEA 240
           RLLL+EPE F++LWDWSCFLD +K               PD+ WCG+Q L +LL LGY+A
Sbjct: 181 RLLLMEPEIFSKLWDWSCFLDLVKDTHK-----------PDLTWCGVQTLRVLLKLGYKA 240

Query: 241 TTNLLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVK-ESVSQGMKTDFDSENCL--- 300
           T NL  G EE  +CLL WEEFCQD S+EKAG Y +      S S   + DF + NCL   
Sbjct: 241 TENLNIGDEEAFSCLLCWEEFCQDTSLEKAGWYVKPIADIMSDSLDKRMDFKNGNCLKSF 300

Query: 301 -LANSSFCGPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQK 360
            L N     P +L    E++   +SQ L T       N+F  TS++K+ Y+ ++LA+SQK
Sbjct: 301 GLNNQLVSSPKLL----ELQPRFKSQLLTTRDDVSVSNTFVFTSAVKRSYDRVLLAVSQK 360

Query: 361 WPVLLYGPPGAGKSALINKIASDSHNQGSN 383
           WPVLLYGP G+GKSALI K+A DS NQG N
Sbjct: 361 WPVLLYGPSGSGKSALIAKLAQDSGNQGQN 372

BLAST of Csa7G450650 vs. TrEMBL
Match: A0A0B2NWG0_GLYSO (Midasin OS=Glycine soja GN=glysoja_009586 PE=3 SV=1)

HSP 1 Score: 32.0 bits (71), Expect = 2.1e+03
Identity = 12/44 (27.27%), Postives = 29/44 (65.91%), Query Frame = 1

Query: 331 SFFMTSSIKKGYEMIMLALSQKWPVLLYGPPGAGKSALINKIAS 375
           ++ +T S+++    +  ++  ++PVLL GP  +GK++L+  +A+
Sbjct: 947 NYVLTKSVQEHLGNLARSVLMRYPVLLQGPTSSGKTSLVRYLAA 990


HSP 2 Score: 380.2 bits (975), Expect = 3.1e-102
Identity = 200/388 (51.55%), Postives = 268/388 (69.07%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           M++DGSF LE +++RF +RCP +     F++++ KG  VTEEEV+S LAEL L+P Y++ 
Sbjct: 1   MSMDGSFSLELSLQRFFARCPKVGRVRRFQSLVEKGCLVTEEEVVSSLAELLLHPKYTIP 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDG--VVNIISFYDQRGRGVD 120
           L+GCFRP+A+++VD+ + LLR VP+LRSN   S      LD   V+N+I F+ Q GRG+D
Sbjct: 61  LIGCFRPIARRVVDKAVTLLRLVPSLRSNLGASVAESGYLDDEEVINVIEFHIQHGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNK---GGLLAVRL 180
           LHELACLAFCRALDLAPFLL + LNYFKFAP PF RI++KG+  E+  +     L  VR 
Sbjct: 121 LHELACLAFCRALDLAPFLLGSALNYFKFAPPPFERILMKGSDLELSAEVITHYLHVVRT 180

Query: 181 SYRLLLVEPETFTQLWDWSCFLDFMKQLPNID--LDNARFLLLPDIRWCGIQILSILLNL 240
           SYRLL+VE   F++ WDWSCFLDF++++ N+D   D +    + DIRWCGI+ILS++LN+
Sbjct: 181 SYRLLVVETNVFSKYWDWSCFLDFVREVVNLDQGSDISFQKDISDIRWCGIRILSVILNM 240

Query: 241 GYEATTNLLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESV-SQGMKTDFDSENC 300
             +A +    G+EE  +C LRWEEFCQD+++EKAG+Y   F    + S+ ++  F  EN 
Sbjct: 241 NDKAVSKFGVGAEEAHSCFLRWEEFCQDIAIEKAGAYFGTFEHNKLDSENIELSFSQEN- 300

Query: 301 LLANSSFCGPAIL--SNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALS 360
                  CG      S   E E P+R+QRL  WG   A   F MTS  K+ +EM++LA+S
Sbjct: 301 ---YQQSCGLCSFTSSQFHESEPPLRNQRLVEWGDKSAGIPFVMTSRSKRSFEMVLLAVS 360

Query: 361 QKWPVLLYGPPGAGKSALINKIASDSHN 379
           QKWPVLLYGP GAGKSALI+K+ASDS N
Sbjct: 361 QKWPVLLYGPAGAGKSALISKMASDSGN 384

BLAST of Csa7G450650 vs. TrEMBL
Match: A0A0D2UPS0_GOSRA (Midasin OS=Gossypium raimondii GN=B456_011G062600 PE=3 SV=1)

HSP 1 Score: 32.0 bits (71), Expect = 2.1e+03
Identity = 15/45 (33.33%), Postives = 30/45 (66.67%), Query Frame = 1

Query: 331  SFFMTSSIKKGYEMIMLALS-QKWPVLLYGPPGAGKSALINKIAS 375
            ++ +T +IKK    +  A+  +++PVLL GP  +GK++L+  +A+
Sbjct: 1069 NYVLTETIKKHLRNLSRAVFIKRYPVLLQGPTSSGKTSLVQYLAT 1113


HSP 2 Score: 29.6 bits (65), Expect = 1.0e+04
Identity = 14/37 (37.84%), Postives = 22/37 (59.46%), Query Frame = 1

Query: 333  FMTSSIKKGYEMIMLALSQKWPVLLYGPPGAGKSALI 370
            F+  + +K    ++ A+    PVLL G PG GK++LI
Sbjct: 1779 FLAPTTRKNALRMLRAMRLSKPVLLEGSPGVGKTSLI 1815


HSP 3 Score: 379.4 bits (973), Expect = 5.3e-102
Identity = 202/403 (50.12%), Postives = 274/403 (67.99%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           MAIDGSF LES +ERFL+RCP L     F+++++K   +TEE V++LLAELF++P Y++ 
Sbjct: 1   MAIDGSFSLESQLERFLARCPKLACIPQFDSLLKKREMMTEERVVNLLAELFIHPKYTIP 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLR--SNSTYSEIHEKEL---------DGVVNIISF 120
           ++GCFRP+ +KIVD+ + LLR VPNLR  SN T +E+ E +L          G  ++I F
Sbjct: 61  MMGCFRPICRKIVDKAVALLRLVPNLRYNSNGTLTEVEEDKLLGEAGNFEDAGDASVIEF 120

Query: 121 YDQRGRGVDLHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGG 180
           + + G G++LHELACLAFCRALDLAPFLL + L+YF+FAP PF R+++KG+ SE+  K G
Sbjct: 121 FARNGWGLNLHELACLAFCRALDLAPFLLGSTLSYFRFAPPPFERMLIKGSVSELPAKAG 180

Query: 181 ---LLAVRLSYRLLLVEPETFTQLWDWSCFLDFMKQLPNIDL-DNARFLL-LPDIRWCGI 240
              L +V+ +YR LL+EP+ F++LWDWSCFLD +++L N+D+ D+ +F+  + DIRWCG+
Sbjct: 181 KHLLNSVQTAYRFLLMEPDVFSRLWDWSCFLDLVQKLANLDMVDDDKFVKNISDIRWCGL 240

Query: 241 QILSILLNLGYEATTNLLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESV-SQGM 300
           QIL ++L +   A  N   G+EE L CLLRWEE C+DV+ EKAG Y E   ++   S   
Sbjct: 241 QILFVILKMNDTAIANFGIGAEEALGCLLRWEELCEDVAQEKAGWYFESSKQQKPDSINP 300

Query: 301 KTDFDSENCL-------LANSSFCGPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTS 360
             DFD ENCL       LA SS       S   E +   R +RL TW      N   +T 
Sbjct: 301 DIDFDQENCLRFFGLNNLAVSS-------SQFPEAQPSTRIRRLVTWNGDSCGNPLVLTL 360

Query: 361 SIKKGYEMIMLALSQKWPVLLYGPPGAGKSALINKIASDSHNQ 380
           S+KK YEM++LA+SQKWPVLLYGP G GK+ALI+K+A DS NQ
Sbjct: 361 SLKKSYEMVLLAVSQKWPVLLYGPAGVGKTALISKLARDSGNQ 396

BLAST of Csa7G450650 vs. TrEMBL
Match: F6HXY6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g01610 PE=3 SV=1)

HSP 1 Score: 31.6 bits (70), Expect = 2.7e+03
Identity = 14/45 (31.11%), Postives = 30/45 (66.67%), Query Frame = 1

Query: 331  SFFMTSSIKKGYEMIMLA-LSQKWPVLLYGPPGAGKSALINKIAS 375
            ++ +T S+++    +  A L +++PVLL GP  +GK++L+  +A+
Sbjct: 981  NYILTKSVREHLRNLARAVLIKRYPVLLQGPTSSGKTSLVQYLAA 1025


HSP 2 Score: 377.9 bits (969), Expect = 1.5e-101
Identity = 200/387 (51.68%), Postives = 257/387 (66.41%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           MAIDGSF L S++ RFL RCP L    P E++ R G  VTEEEV+++L  +FL+P+Y++ 
Sbjct: 1   MAIDGSFSLHSSLRRFLDRCPKLQCFPPLESLERMGDLVTEEEVVNVLVGVFLHPSYTIP 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRS---NSTYSEIHEKELDGVVNIISFYDQRGRGV 120
           L+GCFRP+AQ IVD+ + LLR VPNL S   ++   +  ++ LDGVVN+I FY Q+GRG+
Sbjct: 61  LIGCFRPIAQHIVDKAVALLRLVPNLMSIPKDTAREDDRDRVLDGVVNVIEFYSQQGRGL 120

Query: 121 DLHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSY 180
           DLHELACLAFCRALD+ PFLL +IL+YF FAP PF R  VK  + E      L A ++SY
Sbjct: 121 DLHELACLAFCRALDMGPFLLSSILSYFNFAPAPFERFSVKQFTIETRE---LHAAQISY 180

Query: 181 RLLLVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEA 240
           RLLL+EPE F++LWDWSCFLD +K               PD+ WCG+Q L +LL LGY+A
Sbjct: 181 RLLLMEPEIFSKLWDWSCFLDLVKDTHK-----------PDLTWCGVQTLRVLLKLGYKA 240

Query: 241 TTNLLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVK-ESVSQGMKTDFDSENCL--- 300
           T NL  G EE  +CLL WEEFCQD S+EKAG Y +      S S   + DF + NCL   
Sbjct: 241 TENLNIGDEEAFSCLLCWEEFCQDTSLEKAGWYVKPIADIMSDSLDKRMDFKNGNCLKSF 300

Query: 301 -LANSSFCGPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQK 360
            L N     P +L    E++   +SQ L T       N+F  TS++K+ Y+ ++LA+SQK
Sbjct: 301 GLNNQLVSSPKLL----ELQPRFKSQLLTTRDDVSVSNTFVFTSAVKRSYDRVLLAVSQK 360

Query: 361 WPVLLYGPPGAGKSALINKIASDSHNQ 380
           WPVLLYGP G+GKSALI K+A DS NQ
Sbjct: 361 WPVLLYGPSGSGKSALIAKLAQDSGNQ 369

BLAST of Csa7G450650 vs. TAIR10
Match: AT1G67120.1 (AT1G67120.1 ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;transcription factor binding)

HSP 1 Score: 287.7 bits (735), Expect = 1.1e-77
Identity = 172/387 (44.44%), Postives = 229/387 (59.17%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTE-EEVLSLLAELFLNPNYSV 60
           MAIDGSF L+ A+E F  RCP +     F +I+ KG  V + EEV+  L + FL+P ++V
Sbjct: 1   MAIDGSFNLKLALETFSVRCPKVAAFPCFTSILSKGGEVVDNEEVIHALGDAFLHPEFTV 60

Query: 61  CLLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGV----VNIISFYDQRGR 120
            L+ CF P+ + +VDRV+GLLR V +L+S+  YS+     LD      +++I FY +RG+
Sbjct: 61  PLVHCFLPIIRNVVDRVVGLLRLVDDLKSSIDYSDDVSSVLDNAMTEGISVIDFYVRRGQ 120

Query: 121 GVDLHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGG---LLA 180
            ++LHE ACLAF RAL     LL +ILNYF+ AP P+ RI+VK   SE   +     LL 
Sbjct: 121 RLELHECACLAFSRALHFNTSLLGSILNYFEKAPPPYERILVKDIVSESRMEATDAYLLC 180

Query: 181 VRLSYRLLLVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFL-LLPDIRWCGIQILSILL 240
           +R+SYR L++ PE F++LWDWSC+LD MK+L         FL    D  WCGIQILS++L
Sbjct: 181 LRVSYRFLVIRPEVFSKLWDWSCYLDSMKRLSECPRQQRHFLEKYRDAVWCGIQILSVVL 240

Query: 241 NLGYEATTNLLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSEN 300
                         EE L+CLLRWEEFCQD+ +EKAG Y +L    ++           N
Sbjct: 241 RCSDRLAGCFGFEEEEALSCLLRWEEFCQDIEIEKAGLYIQLPTYTALKS-----LQQFN 300

Query: 301 CLLANSSFCGPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQ 360
            L+   +    A L   DE +  IR  RL TW        F + S +KK +EM+ LA+SQ
Sbjct: 301 TLVPGINKRQSAGLEA-DEPQMKIR--RLDTWDVNSFSEPFEIHSRVKKSFEMVSLAVSQ 360

Query: 361 KWPVLLYGPPGAGKSALINKIASDSHN 379
           K PVLLYGP G+GKSALI K+A +S N
Sbjct: 361 KRPVLLYGPSGSGKSALIRKLADESGN 379


HSP 2 Score: 32.3 bits (72), Expect = 8.0e-01
Identity = 23/72 (31.94%), Postives = 36/72 (50.00%), Query Frame = 1

Query: 315  IRSQRLATW------GSPKAENSFFMTSSIKKGYEMIMLALS-QKWPVLLYGPPGAGKSA 374
            IRSQ L  +       S K   S+  T S+      +  A+  +++PVLL GP  +GK++
Sbjct: 1028 IRSQPLQRYLGELKGSSDKFVGSYVKTKSVIDHLNHLAHAIFIKRYPVLLQGPTSSGKTS 1087

Query: 375  LINKIASDSHNQ 380
            L+  +A+ S N+
Sbjct: 1088 LVKYLAAISGNK 1099

BLAST of Csa7G450650 vs. NCBI nr
Match: gi|700190274|gb|KGN45507.1| (hypothetical protein Csa_7G450650 [Cucumis sativus])

HSP 1 Score: 795.0 bits (2052), Expect = 5.8e-227
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC
Sbjct: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH 120
           LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH
Sbjct: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH 120

Query: 121 ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL 180
           ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL
Sbjct: 121 ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL 180

Query: 181 LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN 240
           LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN
Sbjct: 181 LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN 240

Query: 241 LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC 300
           LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC
Sbjct: 241 LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC 300

Query: 301 GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP 360
           GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP
Sbjct: 301 GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP 360

Query: 361 PGAGKSALINKIASDSHNQGSNCFYGSVDMISL 394
           PGAGKSALINKIASDSHNQGSNCFYGSVDMISL
Sbjct: 361 PGAGKSALINKIASDSHNQGSNCFYGSVDMISL 393

BLAST of Csa7G450650 vs. NCBI nr
Match: gi|778729762|ref|XP_011659635.1| (PREDICTED: midasin [Cucumis sativus])

HSP 1 Score: 766.1 bits (1977), Expect = 2.9e-218
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC
Sbjct: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH 120
           LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH
Sbjct: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH 120

Query: 121 ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL 180
           ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL
Sbjct: 121 ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL 180

Query: 181 LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN 240
           LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN
Sbjct: 181 LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN 240

Query: 241 LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC 300
           LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC
Sbjct: 241 LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC 300

Query: 301 GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP 360
           GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP
Sbjct: 301 GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP 360

Query: 361 PGAGKSALINKIASDSHNQ 380
           PGAGKSALINKIASDSHNQ
Sbjct: 361 PGAGKSALINKIASDSHNQ 379

BLAST of Csa7G450650 vs. NCBI nr
Match: gi|778729762|ref|XP_011659635.1| (PREDICTED: midasin [Cucumis sativus])

HSP 1 Score: 35.0 bits (79), Expect = 3.5e+02
Identity = 16/50 (32.00%), Postives = 32/50 (64.00%), Query Frame = 1

Query: 326  PKAENSFFMTSSIKKGYEMIMLA-LSQKWPVLLYGPPGAGKSALINKIAS 375
            P+   ++ +T S+K+    +  A L +++PVLL GP  +GK++L+  +A+
Sbjct: 1056 PELSENYVLTKSVKEHLRNLARAVLIKRYPVLLQGPTSSGKTSLVKYLAA 1105


HSP 2 Score: 30.4 bits (67), Expect = 8.7e+03
Identity = 15/37 (40.54%), Postives = 21/37 (56.76%), Query Frame = 1

Query: 333  FMTSSIKKGYEMIMLALSQKWPVLLYGPPGAGKSALI 370
            FM  +  K    ++ A+    PVLL G PG GK++LI
Sbjct: 1781 FMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLI 1817


HSP 3 Score: 728.0 bits (1878), Expect = 8.7e-207
Identity = 360/379 (94.99%), Postives = 369/379 (97.36%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           MAIDGSFKLESAVERFLSRCPSLTHQSPF+TII+KGYGVTEEEVLSLLAELFLNPNYSVC
Sbjct: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFDTIIKKGYGVTEEEVLSLLAELFLNPNYSVC 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDGVVNIISFYDQRGRGVDLH 120
           LLGCF P+AQKIVDRVIGLLR VPNLRSNS Y EIHEKELDGVVNIISFYD RGRG+DLH
Sbjct: 61  LLGCFLPLAQKIVDRVIGLLRLVPNLRSNSMYLEIHEKELDGVVNIISFYDHRGRGIDLH 120

Query: 121 ELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSYRLL 180
           ELACLAFCRALDLAPFLLRTIL+YFKFAPCPFHRIVVKG +SEVY+KGGLLAVRLSYRLL
Sbjct: 121 ELACLAFCRALDLAPFLLRTILDYFKFAPCPFHRIVVKGTTSEVYDKGGLLAVRLSYRLL 180

Query: 181 LVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEATTN 240
           LVEPETFTQLWDWSCFLDFMKQLPNIDLD+A+FLLLPDIRWCGIQILSILL LGYEATTN
Sbjct: 181 LVEPETFTQLWDWSCFLDFMKQLPNIDLDHAKFLLLPDIRWCGIQILSILLKLGYEATTN 240

Query: 241 LLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESVSQGMKTDFDSENCLLANSSFC 300
           LLSGSEETLACLLRWEEFCQDVSMEKAG YAELFVKESVSQGMKTDFDSENCLLANSSFC
Sbjct: 241 LLSGSEETLACLLRWEEFCQDVSMEKAGFYAELFVKESVSQGMKTDFDSENCLLANSSFC 300

Query: 301 GPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP 360
           GPAIL NHDEIERPIRSQRLATWGS KAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP
Sbjct: 301 GPAILWNHDEIERPIRSQRLATWGSLKAENSFFMTSSIKKGYEMIMLALSQKWPVLLYGP 360

Query: 361 PGAGKSALINKIASDSHNQ 380
           PGAGKSALINKIASDSHNQ
Sbjct: 361 PGAGKSALINKIASDSHNQ 379

BLAST of Csa7G450650 vs. NCBI nr
Match: gi|659124762|ref|XP_008462334.1| (PREDICTED: LOW QUALITY PROTEIN: midasin [Cucumis melo])

HSP 1 Score: 35.0 bits (79), Expect = 3.5e+02
Identity = 16/50 (32.00%), Postives = 32/50 (64.00%), Query Frame = 1

Query: 326  PKAENSFFMTSSIKKGYEMIMLA-LSQKWPVLLYGPPGAGKSALINKIAS 375
            P+   ++ +T S+K+    +  A L +++PVLL GP  +GK++L+  +A+
Sbjct: 1056 PELSENYVLTKSVKEHLRNLARAVLIKRYPVLLQGPTSSGKTSLVKYLAA 1105


HSP 2 Score: 30.4 bits (67), Expect = 8.7e+03
Identity = 15/37 (40.54%), Postives = 21/37 (56.76%), Query Frame = 1

Query: 333  FMTSSIKKGYEMIMLALSQKWPVLLYGPPGAGKSALI 370
            FM  +  K    ++ A+    PVLL G PG GK++LI
Sbjct: 1781 FMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLI 1817


HSP 3 Score: 382.5 bits (981), Expect = 9.0e-103
Identity = 202/390 (51.79%), Postives = 259/390 (66.41%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           MAIDGSF L S++ RFL RCP L    P E++ R G  VTEEEV+++L  +FL+P+Y++ 
Sbjct: 1   MAIDGSFSLHSSLRRFLDRCPKLQCFPPLESLERMGDLVTEEEVVNVLVGVFLHPSYTIP 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRS---NSTYSEIHEKELDGVVNIISFYDQRGRGV 120
           L+GCFRP+AQ IVD+ + LLR VPNL S   ++   +  ++ LDGVVN+I FY Q+GRG+
Sbjct: 61  LIGCFRPIAQHIVDKAVALLRLVPNLMSIPKDTAREDDRDRVLDGVVNVIEFYSQQGRGL 120

Query: 121 DLHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNKGGLLAVRLSY 180
           DLHELACLAFCRALD+ PFLL +IL+YF FAP PF R  VK  + E      L A ++SY
Sbjct: 121 DLHELACLAFCRALDMGPFLLSSILSYFNFAPAPFERFSVKQFTIETRE---LHAAQISY 180

Query: 181 RLLLVEPETFTQLWDWSCFLDFMKQLPNIDLDNARFLLLPDIRWCGIQILSILLNLGYEA 240
           RLLL+EPE F++LWDWSCFLD +K               PD+ WCG+Q L +LL LGY+A
Sbjct: 181 RLLLMEPEIFSKLWDWSCFLDLVKDTHK-----------PDLTWCGVQTLRVLLKLGYKA 240

Query: 241 TTNLLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVK-ESVSQGMKTDFDSENCL--- 300
           T NL  G EE  +CLL WEEFCQD S+EKAG Y +      S S   + DF + NCL   
Sbjct: 241 TENLNIGDEEAFSCLLCWEEFCQDTSLEKAGWYVKPIADIMSDSLDKRMDFKNGNCLKSF 300

Query: 301 -LANSSFCGPAILSNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALSQK 360
            L N     P +L    E++   +SQ L T       N+F  TS++K+ Y+ ++LA+SQK
Sbjct: 301 GLNNQLVSSPKLL----ELQPRFKSQLLTTRDDVSVSNTFVFTSAVKRSYDRVLLAVSQK 360

Query: 361 WPVLLYGPPGAGKSALINKIASDSHNQGSN 383
           WPVLLYGP G+GKSALI K+A DS NQG N
Sbjct: 361 WPVLLYGPSGSGKSALIAKLAQDSGNQGQN 372

BLAST of Csa7G450650 vs. NCBI nr
Match: gi|734313483|gb|KHN01380.1| (Midasin [Glycine soja])

HSP 1 Score: 32.0 bits (71), Expect = 3.0e+03
Identity = 12/44 (27.27%), Postives = 29/44 (65.91%), Query Frame = 1

Query: 331 SFFMTSSIKKGYEMIMLALSQKWPVLLYGPPGAGKSALINKIAS 375
           ++ +T S+++    +  ++  ++PVLL GP  +GK++L+  +A+
Sbjct: 947 NYVLTKSVQEHLGNLARSVLMRYPVLLQGPTSSGKTSLVRYLAA 990


HSP 2 Score: 380.2 bits (975), Expect = 4.5e-102
Identity = 200/388 (51.55%), Postives = 268/388 (69.07%), Query Frame = 1

Query: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60
           M++DGSF LE +++RF +RCP +     F++++ KG  VTEEEV+S LAEL L+P Y++ 
Sbjct: 1   MSMDGSFSLELSLQRFFARCPKVGRVRRFQSLVEKGCLVTEEEVVSSLAELLLHPKYTIP 60

Query: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNSTYSEIHEKELDG--VVNIISFYDQRGRGVD 120
           L+GCFRP+A+++VD+ + LLR VP+LRSN   S      LD   V+N+I F+ Q GRG+D
Sbjct: 61  LIGCFRPIARRVVDKAVTLLRLVPSLRSNLGASVAESGYLDDEEVINVIEFHIQHGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLRTILNYFKFAPCPFHRIVVKGASSEVYNK---GGLLAVRL 180
           LHELACLAFCRALDLAPFLL + LNYFKFAP PF RI++KG+  E+  +     L  VR 
Sbjct: 121 LHELACLAFCRALDLAPFLLGSALNYFKFAPPPFERILMKGSDLELSAEVITHYLHVVRT 180

Query: 181 SYRLLLVEPETFTQLWDWSCFLDFMKQLPNID--LDNARFLLLPDIRWCGIQILSILLNL 240
           SYRLL+VE   F++ WDWSCFLDF++++ N+D   D +    + DIRWCGI+ILS++LN+
Sbjct: 181 SYRLLVVETNVFSKYWDWSCFLDFVREVVNLDQGSDISFQKDISDIRWCGIRILSVILNM 240

Query: 241 GYEATTNLLSGSEETLACLLRWEEFCQDVSMEKAGSYAELFVKESV-SQGMKTDFDSENC 300
             +A +    G+EE  +C LRWEEFCQD+++EKAG+Y   F    + S+ ++  F  EN 
Sbjct: 241 NDKAVSKFGVGAEEAHSCFLRWEEFCQDIAIEKAGAYFGTFEHNKLDSENIELSFSQEN- 300

Query: 301 LLANSSFCGPAIL--SNHDEIERPIRSQRLATWGSPKAENSFFMTSSIKKGYEMIMLALS 360
                  CG      S   E E P+R+QRL  WG   A   F MTS  K+ +EM++LA+S
Sbjct: 301 ---YQQSCGLCSFTSSQFHESEPPLRNQRLVEWGDKSAGIPFVMTSRSKRSFEMVLLAVS 360

Query: 361 QKWPVLLYGPPGAGKSALINKIASDSHN 379
           QKWPVLLYGP GAGKSALI+K+ASDS N
Sbjct: 361 QKWPVLLYGPAGAGKSALISKMASDSGN 384

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MDN1_HUMAN3.4e-0922.30Midasin OS=Homo sapiens GN=MDN1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KAY4_CUCSA4.0e-227100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G450650 PE=4 SV=1[more]
A0A0B2NWG0_GLYSO6.3e-10351.79Midasin OS=Glycine soja GN=glysoja_009586 PE=3 SV=1[more]
A0A0B2NWG0_GLYSO2.1e+0327.27Midasin OS=Glycine soja GN=glysoja_009586 PE=3 SV=1[more]
A0A0D2UPS0_GOSRA2.1e+0333.33Midasin OS=Gossypium raimondii GN=B456_011G062600 PE=3 SV=1[more]
F6HXY6_VITVI2.7e+0331.11Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g01610 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G67120.11.1e-7744.44 ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;tr... [more]
Match NameE-valueIdentityDescription
gi|700190274|gb|KGN45507.1|5.8e-227100.00hypothetical protein Csa_7G450650 [Cucumis sativus][more]
gi|778729762|ref|XP_011659635.1|2.9e-218100.00PREDICTED: midasin [Cucumis sativus][more]
gi|778729762|ref|XP_011659635.1|3.5e+0232.00PREDICTED: midasin [Cucumis sativus][more]
gi|659124762|ref|XP_008462334.1|3.5e+0232.00PREDICTED: LOW QUALITY PROTEIN: midasin [Cucumis melo][more]
gi|734313483|gb|KHN01380.1|3.0e+0327.27Midasin [Glycine soja][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR012099Midasin
IPR027417P-loop_NTPase
Vocabulary: Biological Process
TermDefinition
GO:0000027ribosomal large subunit assembly
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: Molecular Function
TermDefinition
GO:0016887ATPase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000027 ribosomal large subunit assembly
cellular_component GO:0005634 nucleus
molecular_function GO:0016887 ATPase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa7G450650.1Csa7G450650.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012099MidasinPANTHERPTHR22908:SF66MIDASINcoord: 1..378
score: 4.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 331..376
score: 4.
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 340..389
score: 7.9
NoneNo IPR availablePANTHERPTHR22908MIDASIN-RELATEDcoord: 1..378
score: 4.1

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa7G450650Cla015563Watermelon (97103) v1cuwmB566
Csa7G450650Cp4.1LG12g09060Cucurbita pepo (Zucchini)cpecuB156
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa7G450650Cucurbita maxima (Rimu)cmacuB490
Csa7G450650Cucurbita maxima (Rimu)cmacuB919
Csa7G450650Cucurbita moschata (Rifu)cmocuB035
Csa7G450650Cucurbita moschata (Rifu)cmocuB074
Csa7G450650Cucurbita moschata (Rifu)cmocuB142
Csa7G450650Cucurbita moschata (Rifu)cmocuB261
Csa7G450650Cucurbita moschata (Rifu)cmocuB373
Csa7G450650Cucurbita moschata (Rifu)cmocuB480
Csa7G450650Cucurbita moschata (Rifu)cmocuB905
Csa7G450650Melon (DHL92) v3.5.1cumeB521
Csa7G450650Melon (DHL92) v3.5.1cumeB546
Csa7G450650Melon (DHL92) v3.5.1cumeB559
Csa7G450650Watermelon (Charleston Gray)cuwcgB524
Csa7G450650Watermelon (Charleston Gray)cuwcgB552
Csa7G450650Watermelon (Charleston Gray)cuwcgB558
Csa7G450650Watermelon (Charleston Gray)cuwcgB567
Csa7G450650Watermelon (97103) v1cuwmB580
Csa7G450650Watermelon (97103) v1cuwmB583
Csa7G450650Watermelon (97103) v1cuwmB592
Csa7G450650Cucurbita pepo (Zucchini)cpecuB336
Csa7G450650Cucurbita pepo (Zucchini)cpecuB377
Csa7G450650Cucurbita pepo (Zucchini)cpecuB594
Csa7G450650Cucurbita pepo (Zucchini)cpecuB632
Csa7G450650Cucurbita pepo (Zucchini)cpecuB693
Csa7G450650Bottle gourd (USVL1VR-Ls)culsiB503
Csa7G450650Bottle gourd (USVL1VR-Ls)culsiB525
Csa7G450650Bottle gourd (USVL1VR-Ls)culsiB528
Csa7G450650Bottle gourd (USVL1VR-Ls)culsiB533
Csa7G450650Cucumber (Gy14) v2cgybcuB047
Csa7G450650Cucumber (Gy14) v2cgybcuB149
Csa7G450650Cucumber (Gy14) v2cgybcuB193
Csa7G450650Cucumber (Gy14) v2cgybcuB323
Csa7G450650Melon (DHL92) v3.6.1cumedB501
Csa7G450650Melon (DHL92) v3.6.1cumedB524
Csa7G450650Melon (DHL92) v3.6.1cumedB537
Csa7G450650Silver-seed gourdcarcuB0104
Csa7G450650Silver-seed gourdcarcuB0129
Csa7G450650Silver-seed gourdcarcuB0230
Csa7G450650Silver-seed gourdcarcuB0632
Csa7G450650Silver-seed gourdcarcuB0904
Csa7G450650Silver-seed gourdcarcuB1039
Csa7G450650Watermelon (97103) v2cuwmbB535
Csa7G450650Watermelon (97103) v2cuwmbB543
Csa7G450650Watermelon (97103) v2cuwmbB552
Csa7G450650Watermelon (97103) v2cuwmbB570
Csa7G450650Wax gourdcuwgoB690
Csa7G450650Wax gourdcuwgoB701
Csa7G450650Wax gourdcuwgoB713
Csa7G450650Cucumber (Chinese Long) v2cucuB046
Csa7G450650Cucumber (Chinese Long) v2cucuB154
Csa7G450650Cucumber (Chinese Long) v2cucuB128
Csa7G450650Cucumber (Gy14) v1cgycuB425
Csa7G450650Cucumber (Gy14) v1cgycuB485
Csa7G450650Cucumber (Gy14) v1cgycuB508
Csa7G450650Cucurbita maxima (Rimu)cmacuB047
Csa7G450650Cucurbita maxima (Rimu)cmacuB084
Csa7G450650Cucurbita maxima (Rimu)cmacuB154
Csa7G450650Cucurbita maxima (Rimu)cmacuB273
Csa7G450650Cucurbita maxima (Rimu)cmacuB383