Csa7G430250 (gene) Cucumber (Chinese Long) v2

BLAST of Csa7G430250 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 2.0e-230
Identity = 392/534 (73.41%), Postives = 458/534 (85.77%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQLTARRVAAG 69
           + IFRSKLPDI+IPNHLPLH Y F+N+S+F+SRPCLINGA    YTY DV+L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSSNDVVALPY 189
           VT AC  ++VKD A EN V+++C+D A EGCLHFSVL+ A+E   P V+   +DVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLY H++DV+L VLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSG 309
           LRVGAAILIMQKFDIVS L+LI++++++I P VPPI LAIAKSP  + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAE 369
            APLGKELED VR KFP A LGQGYGMTEAGPVL+M LAFAKEPF++K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDDD 429
           MKIVD +TG SLP N SGEICIRGDQIMKGYLND E+T RT+DKEGWL+TGDIG++DDDD
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVVKANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M D +AGEVPVAFVV++NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 543
             ITE+EVK FI+KQV+FYKR+KRVFFV+AIPK+PSGKILRK+LRAKLA+G  N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of Csa7G430250 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 1.5e-228
Identity = 387/540 (71.67%), Postives = 455/540 (84.26%), Query Frame = 1

Query: 4   ESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQLTA 63
           E+ ++ + IFRSKLPDI+IP HLPLH Y F+N+S+F+SRPCLINGA    YTY +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSSND 183
           ++AK+I+T +CF  +VKD A EN V+V+C+D A EGCLHFS L+ +DE   P V    +D
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGEN NLY H++DV++ VLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSV 303
           SILLCGLRVGAAILIMQKFDI   L+LI+K+++SI P VPPI LAIAKSP  + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIG 423
           VVRNAEMKIVD +TG SLP N  GEICIRGDQIMKGYLND E+T RT+DKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAF 483
           F+D+DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M D +AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 543
           VV++NG AITE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Csa7G430250 vs. Swiss-Prot
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 6.8e-226
Identity = 386/543 (71.09%), Postives = 450/543 (82.87%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           M  E+ ++ + IFRSKLPDI+IP HLPLH Y F+NLS+F SRPCLI+GA    YTY +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AKA++AK+++T ACF  +VKD A EN ++V+CVD A EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGEN NLY H+DDV++ VLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLC LRVGAAILIMQKFDI   L+LI KH+++I P VPPI LAIAKSP    YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD +TG SLP N  GEICIRGDQIMKGYLND E+T RT++KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIGF+DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG  ITE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of Csa7G430250 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 6.8e-226
Identity = 388/540 (71.85%), Postives = 454/540 (84.07%), Query Frame = 1

Query: 7   ETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQLTARRV 66
           +  + IFRSKLPDI+IP +LPLH Y F+N+SKF+SRPCLINGAT + +TY DV+L +RRV
Sbjct: 10  QKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRV 69

Query: 67  AAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANA 126
            +GL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+ KQAKA+NA
Sbjct: 70  GSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNA 129

Query: 127 KLIVTMACFYDRVKDLA-ENGVQVVCVDFAVEG-----CLHFSVLSGADESLAPLVDFSS 186
           KLI+T  C+ D+VKD A ENGV+++ +D           LHFS L+GADE+  P V+ S 
Sbjct: 130 KLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISP 189

Query: 187 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 246
           + VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLY H+DDV+L VLP FHIYS
Sbjct: 190 DGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYS 249

Query: 247 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 306
           LNS+LLCGLR G+ ILIMQKF+IV  L+LI+K++++I P VPPI LAIAKS   + YD+S
Sbjct: 250 LNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLS 309

Query: 307 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 366
           SVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GAC
Sbjct: 310 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 369

Query: 367 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 426
           GTVVRNAEMKIVD ETG+SLP N  GEICIRGDQIMKGYLND E+T RT+DKEGWLHTGD
Sbjct: 370 GTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGD 429

Query: 427 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 486
           IG++DDDDELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVPV
Sbjct: 430 IGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPV 489

Query: 487 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 541
           AFVVK+NG  ITE+E+KQFI+KQV+FYKR+ RVFFV AIPKAPSGKILRK+LRA+LA+ A
Sbjct: 490 AFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLAAAA 549

BLAST of Csa7G430250 vs. Swiss-Prot
Match: 4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 2.0e-225
Identity = 385/543 (70.90%), Postives = 450/543 (82.87%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           M  E+ ++ + IFRSKLPDI+IP HLPLH Y F+NLS+F SRPCLI+GA    YTY +V+
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AKA++AK+++T ACF  +VKD A EN ++V+CVD   EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGEN NLY H+DDV++ VLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLC LRVGAAILIMQKFDI   L+LI KH+++I P VPPI LAIAKSP  + YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD +TG SLP N  GEICIRGDQIMKGYLND E+T RT++KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIGF+DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG  ITE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of Csa7G430250 vs. TrEMBL
Match: A0A0A0K6Q5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 1.8e-278
Identity = 486/545 (89.17%), Postives = 511/545 (93.76%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MAFESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGD YTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV++VCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GAAI+IMQKFDIVSL QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP A+L QGYGMTEAGPVL+MSLAFAKEPFQVK  AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG SLP NS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGF+DD++ELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGA---ITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540
           AFVV+   GA    TEEEVK+FI KQV+FYKRLKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 543
           SGAYN
Sbjct: 541 SGAYN 545

BLAST of Csa7G430250 vs. TrEMBL
Match: B8XPP8_9ROSI (4-coumarate coenzyme A ligase OS=Betula luminifera GN=4CL PE=2 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 9.5e-235
Identity = 400/543 (73.66%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG  YTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD A ENGV+++C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYANENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLYYH++DVIL VLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+ILI+ KF+IVSLLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP QVK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMQVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASL  N  GEICIRGDQIMKGY+ND E+T  T+DKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLARNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DDDDELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  +TE+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of Csa7G430250 vs. TrEMBL
Match: A0A089YQ16_BETPN (4-coumarate:CoA ligase 1 OS=Betula pendula PE=2 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 2.8e-234
Identity = 398/543 (73.30%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG  YTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGV+++C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLYYH++DVIL VLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+ILI+ KF+IVSLLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  T+DKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD+DELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  +TE+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of Csa7G430250 vs. TrEMBL
Match: Q5S017_BETPL (4-coumarate:CoA ligase OS=Betula platyphylla PE=2 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 2.8e-234
Identity = 398/543 (73.30%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG  YTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGV+++C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLYYH++DVIL VLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+ILI+ KF+IVSLLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  T+DKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD+DELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  +TE+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of Csa7G430250 vs. TrEMBL
Match: A0A0H4CUR4_BETPL (4-coumarate-CoA ligase 2 OS=Betula platyphylla GN=4cl2 PE=2 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 1.8e-233
Identity = 397/543 (73.11%), Postives = 469/543 (86.37%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG  YTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL   GI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKPGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGV+++C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLYYH++DVIL VLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+ILI+ KF+IVSLLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  T+DKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD+DELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  +TE+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 YSN 542

BLAST of Csa7G430250 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 748.4 bits (1931), Expect = 3.0e-216
Identity = 370/542 (68.27%), Postives = 452/542 (83.39%), Query Frame = 1

Query: 8   TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQLTARRVA 67
           +N+ IFRS+LPDI+IPNHLPLHDY+F+N+S+FA++PCLING TG+ YTY DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           AGLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVQVVCVDFAV--EGCLHFSVLSGADESLAPLVD-----FSS 187
           LIVT + + D++K+L  +GV +V  D     E CL FS L+ ++E   P VD      S 
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEE---PRVDSIPEKISP 197

Query: 188 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 247
            DVVALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLY++ DDVIL VLP FHIY+
Sbjct: 198 EDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYA 257

Query: 248 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 307
           LNSI+LC LRVGA ILIM KF+I  LL+ I++ ++++  +VPPI LAIAKSPE EKYD+S
Sbjct: 258 LNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLS 317

Query: 308 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 367
           SVR++KSG APLGKELEDA+  KFP A LGQGYGMTEAGPVL+MSL FAKEPF VK+GAC
Sbjct: 318 SVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 377

Query: 368 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 427
           GTVVRNAEMKI+D +TG SLP N  GEICIRG+QIMKGYLND  +T  T+DK+GWLHTGD
Sbjct: 378 GTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGD 437

Query: 428 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 487
           +GF+DDDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M + +AGEVPV
Sbjct: 438 VGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPV 497

Query: 488 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 543
           AFVV++    I+E+E+KQF++KQVVFYKR+ +VFF ++IPKAPSGKILRK+LRA+LA+G 
Sbjct: 498 AFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGL 556

BLAST of Csa7G430250 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 746.9 bits (1927), Expect = 8.8e-216
Identity = 371/544 (68.20%), Postives = 446/544 (81.99%), Query Frame = 1

Query: 5   SNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDY+FQN+S+FA++PCLING TG  YTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDLA-ENGVQVVCVDF-----AVEGCLHFSVLSGADESLAPLVD- 184
           N KLI+T A + D++K L  ++GV +VC+D        EGCL F+ L+ +    + ++D 
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 --FSSNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPF 244
              S +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLY+H+DDVIL VLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFE 304
           FHIY+LNSI+LCGLRVGAAILIM KF+I  LL+LI++ ++++ P+VPPI LAIAKS E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV  KFP A LGQGYGMTEAGPVL+MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGW 424
           K+GACGTVVRNAEMKIVD +TG SL  N  GEICIRG QIMKGYLN+  +T  T+DK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEA 484
           LHTGDIG +DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAK 540
           GEVPVAFVVK+    ++E++VKQF++KQVVFYKR+ +VFF  +IPKAPSGKILRK+LRAK
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAK 556

BLAST of Csa7G430250 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 682.2 bits (1759), Expect = 2.7e-196
Identity = 336/546 (61.54%), Postives = 424/546 (77.66%), Query Frame = 1

Query: 4   ESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKF----ASRPCLINGATGDFYTYHDV 63
           E   +++FIFRSKLPDI IPNHLPL DYVFQ  S      +S  C+I+GATG   TY DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+AAG+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLAENGVQVVCVD---------FAVEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV +VC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SLAPLVDFSSNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVIL 243
           +       S  D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DGENPNL + A+DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 SVLPFFHIYSLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAK 303
             LP FHIY+L++++L  +R GAA+LI+ +F++  +++LI++++++++P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPEFEKYDVSSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAK 363
           SPE E+YD+SSVR++ SG A L KELEDAVR KFP A+ GQGYGMTE+G V + SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFQVKAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTV 423
            PF+ K+GACGTV+RNAEMK+VDTETG SLP N SGEIC+RG Q+MKGYLND E+T RT+
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM 483
           DK+GWLHTGDIGFVDDDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRK 537
            D  A EVPVAFV ++ G  +TE++VK ++ KQVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of Csa7G430250 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 671.4 bits (1731), Expect = 4.7e-193
Identity = 334/527 (63.38%), Postives = 411/527 (77.99%), Query Frame = 1

Query: 12  IFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQLTARRVAAGLH 71
           IFRSKLPDI IPNHLPLH Y F+ LS  + +PCLI G+TG  YTY +  L  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADES--LAPLVDFSSNDVVALPYS 191
            + + D++K+L EN   +   +   E CL FS L   DE+      VD   +D  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG+NPNLY  ++DVIL VLP FHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSGG 311
           R GA +L+M KF+I +LL LI++HR++I  +VPP+ +A+AK+P    YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAEM 371
           APLGKEL+D++R + P A+LGQGYGMTEAGPVLSMSL FAKEP   K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDDDE 431
           K+V  ET  SL  N  GEICIRG QIMK YLND E+T  T+D+EGWLHTGDIG+VD+DDE
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGG 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   D  AGEVPVAFVV++NG 
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 AITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL 537
            ITEE+VK+++ KQVVFYKRL +VFFV +IPK+PSGKILRK+L+AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Csa7G430250 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 402.1 bits (1032), Expect = 5.3e-112
Identity = 223/544 (40.99%), Postives = 333/544 (61.21%), Query Frame = 1

Query: 12  IFRSKLPDIHIPN--HLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQLTARRVAAG 71
           I+RS  P + +P   +  L  ++F+N S + S+  + +  TGD  T+  ++    R+A G
Sbjct: 11  IYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG 70

Query: 72  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 131
            H LGI+K DVV+   PNS +F   FL  +  G + T ANP YT  E++KQ K +N K+I
Sbjct: 71  FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKII 130

Query: 132 VTMACFYDRVKDLAENGVQVVCVDFAV------EGCLHFSVLSGADESLA--PLVDFSSN 191
           +++   +D++K      V +   D            L F  +    E ++  P V+   +
Sbjct: 131 ISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEIKQS 190

Query: 192 DVVALPYSSGTTGLPKGVMLTH-----KGLITSVAQQMDGENPNLYYHADDVILSVLPFF 251
           D  AL YSSGTTG  KGV LTH       L+ ++ Q + GE     YH   V L  LP F
Sbjct: 191 DTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGE-----YHG--VFLCFLPMF 250

Query: 252 HIYSLNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEK 311
           H++ L  I    L+ G A++ M +F++  +L+ IEK R++ + +VPP+FLA++K    +K
Sbjct: 251 HVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKK 310

Query: 312 YDVSSVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVK 371
           +D+SS++ + SG APLGK+L +      P  +L QGYGMTE   ++S+     ++P   K
Sbjct: 311 FDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSV-----EDPRLGK 370

Query: 372 --AGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEG 431
             +G+ G +    E +IV  ETG S P N  GEI +RG  +MKGYLN+ ++TK T+DK+ 
Sbjct: 371 RNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKS 430

Query: 432 WLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVE 491
           W+HTGD+G+ ++D  L++VDR+KELIK+K FQVAPAELE LL++HP + DA VI  PD E
Sbjct: 431 WVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEE 490

Query: 492 AGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRA 539
           AGEVP+AFVV++   +ITE+++++FI KQV  YKRL+RV F++ +PK+ +GKILR+EL  
Sbjct: 491 AGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQ 542

BLAST of Csa7G430250 vs. NCBI nr
Match: gi|778728828|ref|XP_004135893.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 1067.8 bits (2760), Expect = 6.4e-309
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Csa7G430250 vs. NCBI nr
Match: gi|659122577|ref|XP_008461216.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 1043.1 bits (2696), Expect = 1.7e-301
Identity = 527/542 (97.23%), Postives = 535/542 (98.71%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MAFESN+T EFIFRSKLPDIHIPNHLPLH YVFQN+ KFA+RPCLINGATGD YTYHDVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFATRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMACFYDRVKDLAENGV++VCVDFAVEGCLHFSVLSGADESLAP+VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGA ILIMQKFDIVSLLQLIEKH+ISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP AVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Csa7G430250 vs. NCBI nr
Match: gi|659122579|ref|XP_008461217.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1030.0 bits (2662), Expect = 1.5e-297
Identity = 520/542 (95.94%), Postives = 535/542 (98.71%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGD YTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAA+AKLIVTMACFYDRVKDL ENGV++VCVDFAVEGCLHFSVLSGADESLAP VDFSS
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDG+NPNLYYH++DVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVG+AILI+QKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP A+LGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVD+D+ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKA GGAITEEEVKQFITKQVVFYKRLKRV FVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Csa7G430250 vs. NCBI nr
Match: gi|659122581|ref|XP_008461218.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 975.3 bits (2520), Expect = 4.3e-281
Identity = 489/542 (90.22%), Postives = 514/542 (94.83%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MAFESN+T+EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGD Y+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
            KAANAKLIVTMACFYDRVKDLAENGV++VCVDF+VEGCLHFSVLSGADESL P VDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GAAI IMQKFDIVSL QLI K+++SI+PIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP A+L QGYGMTEAGPVL+MSLAFAKEPFQVKA AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLP NS GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDD++ELFI+DRLKELIKFK FQVAPAELEALLI HPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVV+   GA TEEEVKQF+TKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVEEKSGATTEEEVKQFVTKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Csa7G430250 vs. NCBI nr
Match: gi|449436228|ref|XP_004135895.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 966.1 bits (2496), Expect = 2.6e-278
Identity = 486/545 (89.17%), Postives = 511/545 (93.76%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60
           MAFESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGD YTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV++VCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GAAI+IMQKFDIVSL QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP A+L QGYGMTEAGPVL+MSLAFAKEPFQVK  AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG SLP NS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGF+DD++ELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGA---ITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540
           AFVV+   GA    TEEEVK+FI KQV+FYKRLKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 543
           SGAYN
Sbjct: 541 SGAYN 545