Csa7G430240 (gene) Cucumber (Chinese Long) v2

BLAST of Csa7G430240 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 1.7e-229
Identity = 389/534 (72.85%), Postives = 458/534 (85.77%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 69
           + IFRSKLPDI+IPNHLPLH Y F+N+S+F+SRPCLINGA   +YTY DV+L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSSNDVVALPY 189
           VT AC  ++VKD A EN V+I+C+D A EGCLHFSVL+ A+E   P V+   +DVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY H  DV+LCVLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSG 309
           LRVGAAI+IMQKFDIV+ L+LI++++++I P VPPI LAIAKSP  + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAE 369
            APLGKELED VR KFP A LGQGYGMTEAGPVL+M LAFAKEPF++K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDN 429
           MKIVD +TG SLP N SGEICIRGDQIMKGYLND E+T RTIDKEGWL+TGDIG++DDD+
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVMKANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M D +AGEVPVAFV+++NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 543
             I+E+EVK FI+KQV+FYKR+KRVFFV+AIPK+PSGKILRK+LRAKLA+G  N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of Csa7G430240 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 9.4e-228
Identity = 383/540 (70.93%), Postives = 455/540 (84.26%), Query Frame = 1

Query: 4   ESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTA 63
           E+ ++ + IFRSKLPDI+IP HLPLH Y F+N+S+F+SRPCLINGA   +YTY +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSSND 183
           ++AK+I+T +CF  +VKD A EN V+++C+D A EGCLHFS L+ +DE   P V    +D
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY H  DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSV 303
           SILLCGLRVGAAI+IMQKFDI   L+LI+K+++SI P VPPI LAIAKSP  + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKIVD +TG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAF 483
           F+D+D+ELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M D +AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 543
           V+++NG AI+E+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Csa7G430240 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 5.7e-225
Identity = 385/540 (71.30%), Postives = 455/540 (84.26%), Query Frame = 1

Query: 7   ETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRV 66
           +  + IFRSKLPDI+IP +LPLH Y F+N+SKF+SRPCLINGAT +++TY DV+L +RRV
Sbjct: 10  QKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRV 69

Query: 67  AAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANA 126
            +GL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+ KQAKA+NA
Sbjct: 70  GSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNA 129

Query: 127 KLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEG-----CLHFSVLSGADESLAPLVDFSS 186
           KLI+T  C+ D+VKD A ENGV+I+ +D           LHFS L+GADE+  P V+ S 
Sbjct: 130 KLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISP 189

Query: 187 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 246
           + VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY H +DV+LCVLP FHIYS
Sbjct: 190 DGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYS 249

Query: 247 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 306
           LNS+LLCGLR G+ I+IMQKF+IV  L+LI+K++++I P VPPI LAIAKS   + YD+S
Sbjct: 250 LNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLS 309

Query: 307 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 366
           SVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GAC
Sbjct: 310 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 369

Query: 367 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 426
           GTVVRNAEMKIVD ETG+SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGD
Sbjct: 370 GTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGD 429

Query: 427 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 486
           IG++DDD+ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVPV
Sbjct: 430 IGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPV 489

Query: 487 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 541
           AFV+K+NG  I+E+E+KQFI+KQV+FYKR+ RVFFV AIPKAPSGKILRK+LRA+LA+ A
Sbjct: 490 AFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLAAAA 549

BLAST of Csa7G430240 vs. Swiss-Prot
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.3e-224
Identity = 381/543 (70.17%), Postives = 451/543 (83.06%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           M  E+ ++ + IFRSKLPDI+IP HLPLH Y F+NLS+F SRPCLI+GA   +YTY +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AKA++AK+++T ACF  +VKD A EN ++++CVD A EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY H +DV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLC LRVGAAI+IMQKFDI   L+LI KH+++I P VPPI LAIAKSP    YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD +TG SLP N  GEICIRGDQIMKGYLND E+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIGF+DDD+ELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV+++NG  I+E+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of Csa7G430240 vs. Swiss-Prot
Match: 4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 1.7e-224
Identity = 378/531 (71.19%), Postives = 449/531 (84.56%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 69
           + IFRSKLPDI+IP HLPLH Y F+N+SK   + CLINGATG+ +TY  V+L +R+VA+G
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASG 69

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           L+ LGI++GD +M LLPNSPE+ F FLGASYRGAI T ANPF+T+ E+ KQ KA+ AKLI
Sbjct: 70  LNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLI 129

Query: 130 VTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSSNDVVALPY 189
           +T AC+ D+VKD A E  +QI+C+D A + CLHFS L  ADES  P V  +S+DVVALPY
Sbjct: 130 ITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVVALPY 189

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY H  DV++C+LP FHIYSLN++L CG
Sbjct: 190 SSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCG 249

Query: 250 LRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSG 309
           LR G  I+IMQKFDIV  L+LI+K++++I P VPPI LAIAKSP  +KYD+SSVR + SG
Sbjct: 250 LRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSG 309

Query: 310 GAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAE 369
            APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEP+++K+GACGTVVRNAE
Sbjct: 310 AAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAE 369

Query: 370 MKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDN 429
           MKIVD ET ASLP N  GEICIRGDQIMKGYLND EST+ TID+EGWLHTGDIGF+DDD+
Sbjct: 370 MKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDD 429

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVMKANG 489
           ELFIVDRLKE+IK+K FQVAPAELEALL+THP +SDAAV+ M D +AGEVPVAFV++ NG
Sbjct: 430 ELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNG 489

Query: 490 GAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
              +EEE+KQF++KQVVFYKR+ RVFFV+AIPK+PSGKILRK+LRA++ASG
Sbjct: 490 FTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASG 540

BLAST of Csa7G430240 vs. TrEMBL
Match: A0A0A0K6Q5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 1.9e-280
Identity = 491/545 (90.09%), Postives = 514/545 (94.31%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV+IVCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GAAIMIMQKFDIV+L QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVK  AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG SLP NS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGF+DD+NELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVMKANGGA---ISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540
           AFV++   GA    +EEEVK+FIAKQV+FYKRLKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 543
           SGAYN
Sbjct: 541 SGAYN 545

BLAST of Csa7G430240 vs. TrEMBL
Match: B8XPP8_9ROSI (4-coumarate coenzyme A ligase OS=Betula luminifera GN=4CL PE=2 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 1.6e-234
Identity = 399/543 (73.48%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD A ENGV+I+C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYANENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP QVK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMQVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASL  N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLARNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DDD+ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  ++E+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of Csa7G430240 vs. TrEMBL
Match: A0A089YQ16_BETPN (4-coumarate:CoA ligase 1 OS=Betula pendula PE=2 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 4.7e-234
Identity = 397/543 (73.11%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGV+I+C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD++ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  ++E+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of Csa7G430240 vs. TrEMBL
Match: Q5S017_BETPL (4-coumarate:CoA ligase OS=Betula platyphylla PE=2 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 4.7e-234
Identity = 397/543 (73.11%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGV+I+C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD++ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  ++E+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of Csa7G430240 vs. TrEMBL
Match: A0A0H4CUR4_BETPL (4-coumarate-CoA ligase 2 OS=Betula platyphylla GN=4cl2 PE=2 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 3.0e-233
Identity = 396/543 (72.93%), Postives = 469/543 (86.37%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL   GI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKPGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGV+I+C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD++ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  ++E+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 YSN 542

BLAST of Csa7G430240 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 752.3 bits (1941), Expect = 2.1e-217
Identity = 371/542 (68.45%), Postives = 455/542 (83.95%), Query Frame = 1

Query: 8   TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVA 67
           +N+ IFRS+LPDI+IPNHLPLHDY+F+N+S+FA++PCLING TG+VYTY DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           AGLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVQIVCVDFAV--EGCLHFSVLSGADESLAPLVD-----FSS 187
           LIVT + + D++K+L  +GV IV  D     E CL FS L+ ++E   P VD      S 
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEE---PRVDSIPEKISP 197

Query: 188 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 247
            DVVALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY++R+DVILCVLP FHIY+
Sbjct: 198 EDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYA 257

Query: 248 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 307
           LNSI+LC LRVGA I+IM KF+I  LL+ I++ ++++  +VPPI LAIAKSPE EKYD+S
Sbjct: 258 LNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLS 317

Query: 308 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 367
           SVR++KSG APLGKELEDA+  KFP A LGQGYGMTEAGPVL+MSL FAKEPF VK+GAC
Sbjct: 318 SVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 377

Query: 368 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 427
           GTVVRNAEMKI+D +TG SLP N  GEICIRG+QIMKGYLND  +T  TIDK+GWLHTGD
Sbjct: 378 GTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGD 437

Query: 428 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 487
           +GF+DDD+ELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M + +AGEVPV
Sbjct: 438 VGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPV 497

Query: 488 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 543
           AFV+++    ISE+E+KQF++KQVVFYKR+ +VFF ++IPKAPSGKILRK+LRA+LA+G 
Sbjct: 498 AFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGL 556

BLAST of Csa7G430240 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 747.7 bits (1929), Expect = 5.2e-216
Identity = 371/544 (68.20%), Postives = 447/544 (82.17%), Query Frame = 1

Query: 5   SNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDY+FQN+S+FA++PCLING TG VYTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDLA-ENGVQIVCVDF-----AVEGCLHFSVLSGADESLAPLVD- 184
           N KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    + ++D 
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 --FSSNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPF 244
              S +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+H +DVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFE 304
           FHIY+LNSI+LCGLRVGAAI+IM KF+I  LL+LI++ ++++ P+VPPI LAIAKS E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV  KFP A LGQGYGMTEAGPVL+MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVD +TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEA 484
           LHTGDIG +DDD+ELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAK 540
           GEVPVAFV+K+    +SE++VKQF++KQVVFYKR+ +VFF  +IPKAPSGKILRK+LRAK
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAK 556

BLAST of Csa7G430240 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 682.9 bits (1761), Expect = 1.6e-196
Identity = 336/546 (61.54%), Postives = 425/546 (77.84%), Query Frame = 1

Query: 4   ESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKF----ASRPCLINGATGDVYTYHDV 63
           E   +++FIFRSKLPDI IPNHLPL DYVFQ  S      +S  C+I+GATG + TY DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+AAG+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLAENGVQIVCVD---------FAVEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SLAPLVDFSSNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVIL 243
           +       S  D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL +  NDVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAK 303
           C LP FHIY+L++++L  +R GAA++I+ +F++  +++LI++++++++P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPEFEKYDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAK 363
           SPE E+YD+SSVR++ SG A L KELEDAVR KFP AI GQGYGMTE+G V + SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFQVKAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTI 423
            PF+ K+GACGTV+RNAEMK+VDTETG SLP N SGEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM 483
           DK+GWLHTGDIGFVDDD+E+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDVEAGEVPVAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRK 537
            D  A EVPVAFV ++ G  ++E++VK ++ KQVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of Csa7G430240 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 673.7 bits (1737), Expect = 9.5e-194
Identity = 337/527 (63.95%), Postives = 411/527 (77.99%), Query Frame = 1

Query: 12  IFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAGLH 71
           IFRSKLPDI IPNHLPLH Y F+ LS  + +PCLI G+TG  YTY +  L  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADES--LAPLVDFSSNDVVALPYS 191
            + + D++K+L EN   I   +   E CL FS L   DE+      VD   +D  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY   NDVILCVLP FHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSGG 311
           R GA +++M KF+I ALL LI++HR++I  +VPP+ +A+AK+P    YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAEM 371
           APLGKEL+D++R + P AILGQGYGMTEAGPVLSMSL FAKEP   K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDNE 431
           K+V  ET  SL  N  GEICIRG QIMK YLND E+T  TID+EGWLHTGDIG+VD+D+E
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVMKANGG 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   D  AGEVPVAFV+++NG 
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 AISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL 537
            I+EE+VK+++AKQVVFYKRL +VFFV +IPK+PSGKILRK+L+AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Csa7G430240 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 407.9 bits (1047), Expect = 9.7e-114
Identity = 222/539 (41.19%), Postives = 332/539 (61.60%), Query Frame = 1

Query: 12  IFRSKLPDIHIPN--HLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 71
           I+RS  P + +P   +  L  ++F+N S + S+  + +  TGD  T+  ++    R+A G
Sbjct: 11  IYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG 70

Query: 72  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 131
            H LGI+K DVV+   PNS +F   FL  +  G + T ANP YT  E++KQ K +N K+I
Sbjct: 71  FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKII 130

Query: 132 VTMACFYDRVKDLAENGVQIVCVDFAV------EGCLHFSVLSGADESLA--PLVDFSSN 191
           +++   +D++K      V +   D            L F  +    E ++  P V+   +
Sbjct: 131 ISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEIKQS 190

Query: 192 DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYSL 251
           D  AL YSSGTTG  KGV LTH   I   A  M   + +L    + V LC LP FH++ L
Sbjct: 191 DTAALLYSSGTTGTSKGVELTHGNFIA--ASLMVTMDQDLMGEYHGVFLCFLPMFHVFGL 250

Query: 252 NSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSS 311
             I    L+ G A++ M +F++  +L+ IEK R++ + +VPP+FLA++K    +K+D+SS
Sbjct: 251 AVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFDLSS 310

Query: 312 VRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVK--AGA 371
           ++ + SG APLGK+L +      P  +L QGYGMTE   ++S+     ++P   K  +G+
Sbjct: 311 LKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSV-----EDPRLGKRNSGS 370

Query: 372 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 431
            G +    E +IV  ETG S P N  GEI +RG  +MKGYLN+ ++TK TIDK+ W+HTG
Sbjct: 371 AGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTG 430

Query: 432 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 491
           D+G+ ++D  L++VDR+KELIK+K FQVAPAELE LL++HP + DA VI  PD EAGEVP
Sbjct: 431 DLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVP 490

Query: 492 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLAS 539
           +AFV+++   +I+E+++++FIAKQV  YKRL+RV F++ +PK+ +GKILR+EL  ++ S
Sbjct: 491 IAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRS 542

BLAST of Csa7G430240 vs. NCBI nr
Match: gi|659122581|ref|XP_008461218.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 977.2 bits (2525), Expect = 1.1e-281
Identity = 491/542 (90.59%), Postives = 515/542 (95.02%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+T+EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
            KAANAKLIVTMACFYDRVKDLAENGV+IVCVDF+VEGCLHFSVLSGADESL P VDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GAAI IMQKFDIV+L QLI K+++SI+PIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVKA AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLP NS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDD+NELFI+DRLKELIKFK FQVAPAELEALLI HPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFV++   GA +EEEVKQF+ KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVEEKSGATTEEEVKQFVTKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Csa7G430240 vs. NCBI nr
Match: gi|449436228|ref|XP_004135895.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 972.6 bits (2513), Expect = 2.8e-280
Identity = 491/545 (90.09%), Postives = 514/545 (94.31%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV+IVCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GAAIMIMQKFDIV+L QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVK  AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG SLP NS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGF+DD+NELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVMKANGGA---ISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540
           AFV++   GA    +EEEVK+FIAKQV+FYKRLKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 543
           SGAYN
Sbjct: 541 SGAYN 545

BLAST of Csa7G430240 vs. NCBI nr
Match: gi|212960469|gb|ACJ38668.1| (4-coumarate coenzyme A ligase [Betula luminifera])

HSP 1 Score: 820.1 bits (2117), Expect = 2.3e-234
Identity = 399/543 (73.48%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD A ENGV+I+C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYANENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP QVK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMQVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASL  N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLARNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DDD+ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  ++E+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of Csa7G430240 vs. NCBI nr
Match: gi|55775693|gb|AAV65114.1| (4-coumarate:CoA ligase [Betula platyphylla])

HSP 1 Score: 818.5 bits (2113), Expect = 6.8e-234
Identity = 397/543 (73.11%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGV+I+C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD++ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  ++E+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of Csa7G430240 vs. NCBI nr
Match: gi|854931009|gb|AKN79312.1| (4-coumarate-CoA ligase 2 [Betula platyphylla])

HSP 1 Score: 815.8 bits (2106), Expect = 4.4e-233
Identity = 396/543 (72.93%), Postives = 469/543 (86.37%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL   GI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKPGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGV+I+C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD++ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFV ++NG  ++E+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKELRAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 YSN 542