Csa7G398220 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa7G398220
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Polygalacturonase; contains IPR000743 (Glycoside hydrolase, family 28), IPR011050 (Pectin lyase fold/virulence factor)
Location	Chr7 : 15414024 .. 15415775 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAAGAAATTTTAGCCTCATTCAAATCCTTCTGTTAGTATTTGCCTGGGAATGCTGTGCAAGGGCTGCTGCCATTTTCATTGACACCATCAACACTCAAATTCTTCTCAACGGGATTGTACCAAAGGTAAATGAGCAGTTTCTTCGTCCTGCAGAAGCACCCTGGCATCTTGGCTTCAGTACTCTTCCCAACATTGGCGGCGGCACGGTGAATGACATTAAGGTAAATGTTGATCTAAATGAGAATGGTGGGTTAGTTTTTGATGTTACAAAACATGGAGCTAAAGCTGACGGAGAGACTGATGATGCACAGGTGAGAGATATCAACACACACTAACTTCAAACCTTTTGATTTGAGTTAATTATCTTTAAATTGAAGACTATAGAAAATTGTATATATTTTTTGCTATTAAATTCTACCGTTATATTATTCTAAACTGATGTCAATTTTTCTTCTTTTTTTTTTTTTTTTTNNNNNNNNNNNNATGGTACAATTATTTCAACATTTTTTGGAAAAAAAGAGTTATAATTATTTTTTTTATTTTCCATTTGGCAGGCATTCATGACAACATGGATTGCAGCTTGCCGGAACACAGTCGGTCCGGCGAAGTTCTTGATCCCACAAGACATATTTCTGGTGGGTCCGGTGACTTTCGCCGGTCCGTGCAAAAGCTTTCCAATCACTCTCGAAAATCAAGGAACTGTAAAGGCCACAACCGATATTAGTGCATATTCTTCTTCAGAATGGTTCTCTATTGAAGATGTTACTGGTTTCATCCTCACCGGCTCCGGCGTCTTTGACGGCCAAGGCGTCTCCGTTTGGACCTATAATGACTGCATAAAAAACAACTTATGCCAACTTCTTCCAATCGTAACTATACGTTTGCCTAACTCAATCATTTTTCCTTTCATTTTAAATTACAACTGTTTTACACTCAACTTTTTCAATTTGTGTCTAATAAGTATCTCAAAAAACCTAAACTCTCTAAGAATAAACATATTTAAAAATAAAAATTTATTGCAACTTATATAAAATCACTCAAAATATTTATGAAATATAAAAAAAAAAAAATCATATTCTATCTCTCGGAATCTGAAATTGAATTGTGAAATTTTACTTTACAAACTAATTGTGCTATATTCGAAAATATATTTTTAATGATTATGATTTATTCATTGATTTTCTTTGTAGTCGATTAAATTCTCGAGATTAAATCACACGATAATTGATGGGCTCACTTCGATAAACAGCATGGGGTTTCACACGTCAGTATTCTACTGCTACAATTTTACTGCTACTAACATGAAAATTACAGCTCCACATAATAGTCCCAACACTGATGGAATGCACCTTAGCACCTCCAAGTTGGTCACCATTAGCAACAGTATCATTGGCACCGGTGATGATTGTGTCTCTATTGGCCACAGTACTGAGAATATCATCATCACCAATGTCACTTGCGGCCCTGGTCATGGTCTTAGGTAAATAATACATTTTTATAAAAGTAAATAAAAGATTAAATAGACACAAAGAAAAACATCTAAAAGCTTTTCTTTATTCATTTTTAGTGTGGGCAGCTTGGGCAAGTACTCGAAAGAAAAGGGTGTCTACGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATTAAGACATTTGCTAGCCCTATCCCGGGGTTAGCCTCGAGAATAGTCTTTGAAGACATTGTAATGTACAACGTAAAAATCCTATAA

mRNA sequence

ATGACAAGAAATTTTAGCCTCATTCAAATCCTTCTGTTAGTATTTGCCTGGGAATGCTGTGCAAGGGCTGCTGCCATTTTCATTGACACCATCAACACTCAAATTCTTCTCAACGGGATTGTACCAAAGGTAAATGAGCAGTTTCTTCGTCCTGCAGAAGCACCCTGGCATCTTGGCTTCAGTACTCTTCCCAACATTGGCGGCGGCACGGTGAATGACATTAAGGTAAATGTTGATCTAAATGAGAATGGTGGGTTAGTTTTTGATGTTACAAAACATGGAGCTAAAGCTGACGGAGAGACTGATGATGCACAGGCATTCATGACAACATGGATTGCAGCTTGCCGGAACACAGTCGGTCCGGCGAAGTTCTTGATCCCACAAGACATATTTCTGGTGGGTCCGGTGACTTTCGCCGGTCCGTGCAAAAGCTTTCCAATCACTCTCGAAAATCAAGGAACTGTAAAGGCCACAACCGATATTAGTGCATATTCTTCTTCAGAATGGTTCTCTATTGAAGATGTTACTGGTTTCATCCTCACCGGCTCCGGCGTCTTTGACGGCCAAGGCGTCTCCTCGATTAAATTCTCGAGATTAAATCACACGATAATTGATGGGCTCACTTCGATAAACAGCATGGGGTTTCACACGTCAGTATTCTACTGCTACAATTTTACTGCTACTAACATGAAAATTACAGCTCCACATAATAGTCCCAACACTGATGGAATGCACCTTAGCACCTCCAAGTTGGTCACCATTAGCAACAGTATCATTGGCACCGGTGATGATTGTGTCTCTATTGGCCACAGTACTGAGAATATCATCATCACCAATGTCACTTGCGGCCCTGGTCATGGTCTTAGTGTGGGCAGCTTGGGCAAGTACTCGAAAGAAAAGGGTGTCTACGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATTAAGACATTTGCTAGCCCTATCCCGGGGTTAGCCTCGAGAATAGTCTTTGAAGACATTGTAATGTACAACGTAAAAATCCTATAA

Coding sequence (CDS)

ATGACAAGAAATTTTAGCCTCATTCAAATCCTTCTGTTAGTATTTGCCTGGGAATGCTGTGCAAGGGCTGCTGCCATTTTCATTGACACCATCAACACTCAAATTCTTCTCAACGGGATTGTACCAAAGGTAAATGAGCAGTTTCTTCGTCCTGCAGAAGCACCCTGGCATCTTGGCTTCAGTACTCTTCCCAACATTGGCGGCGGCACGGTGAATGACATTAAGGTAAATGTTGATCTAAATGAGAATGGTGGGTTAGTTTTTGATGTTACAAAACATGGAGCTAAAGCTGACGGAGAGACTGATGATGCACAGGCATTCATGACAACATGGATTGCAGCTTGCCGGAACACAGTCGGTCCGGCGAAGTTCTTGATCCCACAAGACATATTTCTGGTGGGTCCGGTGACTTTCGCCGGTCCGTGCAAAAGCTTTCCAATCACTCTCGAAAATCAAGGAACTGTAAAGGCCACAACCGATATTAGTGCATATTCTTCTTCAGAATGGTTCTCTATTGAAGATGTTACTGGTTTCATCCTCACCGGCTCCGGCGTCTTTGACGGCCAAGGCGTCTCCTCGATTAAATTCTCGAGATTAAATCACACGATAATTGATGGGCTCACTTCGATAAACAGCATGGGGTTTCACACGTCAGTATTCTACTGCTACAATTTTACTGCTACTAACATGAAAATTACAGCTCCACATAATAGTCCCAACACTGATGGAATGCACCTTAGCACCTCCAAGTTGGTCACCATTAGCAACAGTATCATTGGCACCGGTGATGATTGTGTCTCTATTGGCCACAGTACTGAGAATATCATCATCACCAATGTCACTTGCGGCCCTGGTCATGGTCTTAGTGTGGGCAGCTTGGGCAAGTACTCGAAAGAAAAGGGTGTCTACGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATTAAGACATTTGCTAGCCCTATCCCGGGGTTAGCCTCGAGAATAGTCTTTGAAGACATTGTAATGTACAACGTAAAAATCCTATAA

Protein sequence

MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGFSTLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFILTGSGVFDGQGVSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKIL*

Homology

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BLAST of Csa7G398220 vs. Swiss-Prot
Match: PGLR1_ARATH (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana GN=PGA3 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 127/284 (44.72%), Postives = 168/284 (59.15%), Query Frame = 1


Query: 81  NENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAG 140
           N     V+D+TK GA  DG T+  +AF+ TWI  C + V PA  L+P+  FL GPV FAG
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPV-PATLLVPKGTFLAGPVIFAG 104

Query: 141 PCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFILTGSGVFDGQGVS-------- 200
           PCKS  +T+   GT+ ATT  S Y++ EWF  E V   +LTG+G F G+G +        
Sbjct: 105 PCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCG 164

Query: 201 ----------SIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTD 260
                     S+KF  + +  I+G++S+N+  FH  +    N    N+K+TAP  SPNTD
Sbjct: 165 KKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTD 224

Query: 261 GMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGV 320
           G+HLS +  V+I +S I TGDDCVS+G  + N+ +  V CGPGHGLSVGSLGKY  E+ V
Sbjct: 225 GIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDV 284

Query: 321 YDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVK 347
             + V NCT+    NG RIKT+    P  A  I FE+I+M +VK
Sbjct: 285 SGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVK 324

[Close]

BLAST of Csa7G398220 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.6e-58
Identity = 121/274 (44.16%), Postives = 160/274 (58.39%), Query Frame = 1


Query: 90  VTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAGPCKSFPITL 149
           V K GAKADG+TD ++ F+  W  AC + V P+  +IP+  +L+  V   GPCK+ PI +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACAS-VTPSTVVIPKGTYLLSKVNLEGPCKA-PIEI 91

Query: 150 ENQGTVKATTDISAYSSSEWFSIEDVTGFILTGSGVFDGQGVSS---------------- 209
             QGT++A  D SA+    W     V  F + G G+FDGQG  +                
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 210 --IKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTDGMHLSTSKL 269
             I+F  L + +I  +TS +S  FH +VF C N T   +KI AP  SPNTDG+H+  S+ 
Sbjct: 152 VNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 270 VTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCT 329
           V I  S I TGDDC+SIG  T+N++I  +TCGPGHG+S+GSLGK+  E+ V  + + NCT
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 330 IFNATNGARIKTFASPIPGLASRIVFEDIVMYNV 346
           I N +NGARIKT+     G  S I FEDI M NV
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNV 303

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BLAST of Csa7G398220 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-58
Identity = 120/274 (43.80%), Postives = 160/274 (58.39%), Query Frame = 1


Query: 90  VTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAGPCKSFPITL 149
           V K GAKADG+TD ++ F+  W  AC + V P+  +IP+  +L+  V   GPCK+ PI +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACAS-VTPSTVVIPKGTYLLSKVNLEGPCKA-PIEI 91

Query: 150 ENQGTVKATTDISAYSSSEWFSIEDVTGFILTGSGVFDGQGVSS---------------- 209
             QGT++A  D SA+    W     V  F + G G+FDGQG  +                
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 210 --IKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTDGMHLSTSKL 269
             I+F  + + +I  +TS +S  FH +VF C N T   +KI AP  SPNTDG+H+  S+ 
Sbjct: 152 VNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 270 VTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCT 329
           V I  S I TGDDC+SIG  T+N++I  +TCGPGHG+S+GSLGK+  E+ V  + + NCT
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 330 IFNATNGARIKTFASPIPGLASRIVFEDIVMYNV 346
           I N +NGARIKT+     G  S I FEDI M NV
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNV 303

[Close]

BLAST of Csa7G398220 vs. Swiss-Prot
Match: PGLR2_PLAAC (Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.3e-57
Identity = 116/283 (40.99%), Postives = 165/283 (58.30%), Query Frame = 1


Query: 82  ENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAGP 141
           ++ G VF+V  +GAK  G  D +QA M  W AAC +  GP+  LIP+  + +G V   GP
Sbjct: 4   QSSGSVFNVNDYGAKGAG--DISQAVMKAWKAACASQ-GPSTVLIPKGNYNMGEVAMQGP 63

Query: 142 CKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFILTGSGVFDGQGVSS-------- 201
           CK   I  +  G VKA  D S + S  W S   + G  ++G+G  DGQG ++        
Sbjct: 64  CKGSKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDK 123

Query: 202 ----------IKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTDG 261
                     ++F  L H ++  +TS+NS  FH +V  C + T  ++ +TAP  S NTDG
Sbjct: 124 NPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDG 183

Query: 262 MHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGVY 321
           +H+  SK VTI+N+ I TGDDC+SIG  ++N+ IT V CGPGHG+S+GSLG+Y+ EK V 
Sbjct: 184 IHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVR 243

Query: 322 DVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVK 347
            + VK CT     NG R+KT+ +  PG A+ + F+D+ M NV+
Sbjct: 244 GITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQ 283

[Close]

BLAST of Csa7G398220 vs. Swiss-Prot
Match: PGLR3_MAIZE (Exopolygalacturonase OS=Zea mays GN=PG2C PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 116/275 (42.18%), Postives = 160/275 (58.18%), Query Frame = 1


Query: 88  FDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAGPCKSFPI 147
           FD+TK GA  +G+TD  +A    W +AC  T G    LIP+  FLVGP+ F GPCK   +
Sbjct: 41  FDITKLGASGNGKTDSTKAVQEAWASACGGT-GKQTILIPKGDFLVGPLNFTGPCKG-DV 100

Query: 148 TLENQGTVKATTDISAYSS-SEWFSIEDVTGFILTGSGVFDGQGVS-------------- 207
           T++  G + ATTD+S Y     W  I  V   ++TG G  DGQG +              
Sbjct: 101 TIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCK 160

Query: 208 ----SIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTDGMHLST 267
               S+    +N+  + G+T +NS  FH +++ C +    ++ +TAP +SPNTDG+H+  
Sbjct: 161 ILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGD 220

Query: 268 SKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGVYDVLVK 327
           S  VTI+N++IG GDDC+SIG  T  + IT VTCGPGHG+S+GSLG+Y  EK V D+ VK
Sbjct: 221 SSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 280

Query: 328 NCTIFNATNGARIKTFASPIPGL-ASRIVFEDIVM 343
           +CT+    NG RIK +      L AS+I +E+I M
Sbjct: 281 DCTLKKTANGVRIKAYEDAASVLTASKIHYENIKM 313

[Close]

BLAST of Csa7G398220 vs. TrEMBL
Match: A0A0A0K5I2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G398220 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 2.9e-200
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 1


Query: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF 60
           MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF
Sbjct: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF 60

Query: 61  STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120
           STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG
Sbjct: 61  STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120

Query: 121 PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL 180
           PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL
Sbjct: 121 PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL 180

Query: 181 TGSGVFDGQGVSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPN 240
           TGSGVFDGQGVSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPN
Sbjct: 181 TGSGVFDGQGVSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPN 240

Query: 241 TDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEK 300
           TDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEK
Sbjct: 241 TDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEK 300

Query: 301 GVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKIL 349
           GVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKIL
Sbjct: 301 GVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKIL 348

[Close]

BLAST of Csa7G398220 vs. TrEMBL
Match: A0A0A0KMA6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G222970 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 2.8e-155
Identity = 278/328 (84.76%), Postives = 293/328 (89.33%), Query Frame = 1


Query: 37  LNGIVPKVNEQFLRPAEAPWHLGFSTLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAK 96
           L GIVP  N+QFLRPAEAP HLGF+TLP+IGG TVNDI  NVDLNENGG VFDVTKHGAK
Sbjct: 6   LYGIVPTTNKQFLRPAEAPRHLGFNTLPDIGG-TVNDINANVDLNENGGSVFDVTKHGAK 65

Query: 97  ADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVK 156
           ADG+TDDAQAFMTTWIAACRNTVGPAKFLIPQ  FLVGPVTFAGPCKS+PITLENQGTVK
Sbjct: 66  ADGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSYPITLENQGTVK 125

Query: 157 ATTDISAYSSSEWFSIEDVTGFILTGSGVFDGQGVS------------------SIKFSR 216
           ATTDISAYSS EWFS+ED+TGFILTGSGVFDGQG+S                  SIKF+R
Sbjct: 126 ATTDISAYSSPEWFSLEDITGFILTGSGVFDGQGLSVWPYNDCKKNNLCQLLPISIKFTR 185

Query: 217 LNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNSI 276
           LNHTI+DGLTSINSMGFHTSVFYCYNFTATNMKI APHNSPNTDGMHLSTSKLVTI+NSI
Sbjct: 186 LNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSI 245

Query: 277 IGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNG 336
           IGTGDDCVSIGHSTENI +TNVTCGPGHGL   SLGKYSKEKGVYDVLVKNCTIFNATNG
Sbjct: 246 IGTGDDCVSIGHSTENITVTNVTCGPGHGL---SLGKYSKEKGVYDVLVKNCTIFNATNG 305

Query: 337 ARIKTFASPIPGLASRIVFEDIVMYNVK 347
           ARIKT+ASP+ GLASRI+FEDIVMYNVK
Sbjct: 306 ARIKTWASPVSGLASRIIFEDIVMYNVK 329

[Close]

BLAST of Csa7G398220 vs. TrEMBL
Match: A0A0A0K5G0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375750 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.3e-136
Identity = 255/366 (69.67%), Postives = 284/366 (77.60%), Query Frame = 1


Query: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFL-RPAEAPWHLG 60
           ++R  SL QILLLVFAW+C  + AAI  D I    L N I+  +N+Q +  P   P  LG
Sbjct: 3   ISRILSLFQILLLVFAWQCYDKVAAIITDDIAN--LNNEIISTINQQLIPSPTATPGPLG 62

Query: 61  FSTLPNIGGGTVNDIK-VNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNT 120
             TLP++   TVND    N DLNENG  VFDVTKHGAKADG+TDDAQAF TTWIAACRNT
Sbjct: 63  METLPDLDD-TVNDSNNTNNDLNENGESVFDVTKHGAKADGKTDDAQAFETTWIAACRNT 122

Query: 121 VGPAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGF 180
           VGP K LIP+  +LVGP+T AGPCKSFPIT+ENQG VKATTD+S YSS EWFSIED+TGF
Sbjct: 123 VGPVKILIPKGTYLVGPMTLAGPCKSFPITIENQGIVKATTDLSEYSSPEWFSIEDITGF 182

Query: 181 ILTGSGVFDGQGVS------------------SIKFSRLNHTIIDGLTSINSMGFHTSVF 240
           ILTGSGVFDGQG +                  SIKFS LNHTI+DG+TS+NS GFH S+F
Sbjct: 183 ILTGSGVFDGQGTAVWAYNDCKSNKDCQLLPISIKFSGLNHTIVDGITSLNSKGFHMSLF 242

Query: 241 YCYNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNV 300
            CYNFT TN+ I AP  SPNTDG+HLSTS+LV I NSIIGTGDDCVSIGHST  I +TNV
Sbjct: 243 NCYNFTITNVNIIAPDESPNTDGIHLSTSELVNIMNSIIGTGDDCVSIGHSTVKITVTNV 302

Query: 301 TCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDI 347
           TCGPGHGLSVGSLGKYS+EK VYDVLVKNCTIFNATNGARIKTFASPI GLAS I+FEDI
Sbjct: 303 TCGPGHGLSVGSLGKYSREKDVYDVLVKNCTIFNATNGARIKTFASPISGLASGIIFEDI 362

[Close]

BLAST of Csa7G398220 vs. TrEMBL
Match: A0A0A0KAZ2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G398720 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.4e-85
Identity = 164/192 (85.42%), Postives = 169/192 (88.02%), Query Frame = 1


Query: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF 60
           MTRNFSLIQILLLVFA +CCA    IF D   TQ LL GIVPK N+QFLRPAE+P HLGF
Sbjct: 1   MTRNFSLIQILLLVFACQCCA---VIFDDVTGTQNLLKGIVPKANKQFLRPAESPRHLGF 60

Query: 61  STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120
           STLPNIGG  VNDIK NVDLNENGG VFDVTKHGAK DGETDDA AFMTTWIAACRNTVG
Sbjct: 61  STLPNIGG-RVNDIKANVDLNENGGSVFDVTKHGAKGDGETDDAHAFMTTWIAACRNTVG 120

Query: 121 PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL 180
           P KFLIPQ  FLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSS EWFSIED+TGFIL
Sbjct: 121 PTKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFIL 180

Query: 181 TGSGVFDGQGVS 193
           TGSGVFDGQG+S
Sbjct: 181 TGSGVFDGQGLS 188

[Close]

BLAST of Csa7G398220 vs. TrEMBL
Match: W9QFJ0_9ROSA (Exopolygalacturonase clone GBGE184 OS=Morus notabilis GN=L484_011125 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.1e-82
Identity = 159/299 (53.18%), Postives = 202/299 (67.56%), Query Frame = 1


Query: 68  GGTVNDIKVNVDLNENGGLVFDVTKHGAKADGET-DDAQAFMTTWIAACRNTVGPAKFLI 127
           G   +D  +   L E G  +FDVTK+GA A+ E  D+ QAFM TW+AACRN+  PAK +I
Sbjct: 34  GHVSDDTGLRRGLTETGRQIFDVTKYGAVANNERKDNVQAFMKTWVAACRNSNAPAKIVI 93

Query: 128 PQDIFLVGPVTFAGPCKSF-PITLENQGTVKATTDISAYSSSEWFSIEDVTGFILTGSGV 187
           P   FLVGPV F GPCKS  PI +E QGTVKATTD+S YSS EWFS E V G +LTG G 
Sbjct: 94  PTGAFLVGPVVFQGPCKSSEPIVIEVQGTVKATTDVSDYSSPEWFSFEFVNGLVLTGKGT 153

Query: 188 FDGQG------------------VSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTA 247
           FDGQG                   SS++FS +N+  ++G+TS+NS  FH  ++   N T 
Sbjct: 154 FDGQGPKVWHYNDCKTNPNCQHLASSLRFSNVNNAYVEGITSLNSKWFHFFIYESKNITL 213

Query: 248 TNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHG 307
            N+KITAP  SPNTDG+H+S S  V ++N+ I TGDDC+ I     NI ITNVTCGPGHG
Sbjct: 214 NNVKITAPAESPNTDGIHISASTSVNVTNTAIATGDDCIGIVQDCHNIYITNVTCGPGHG 273

Query: 308 LSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVK 347
           +SVGSLGK+S +KGV +++V+NCT+ N TNGARIKT+     G A+ I++E+I+M NVK
Sbjct: 274 ISVGSLGKWSYDKGVSNIVVRNCTLVNTTNGARIKTWGGTTSGEATGIIYENIIMKNVK 332

[Close]

BLAST of Csa7G398220 vs. TAIR10
Match: AT1G02790.1 (AT1G02790.1 polygalacturonase 4)

HSP 1 Score: 241.1 bits (614), Expect = 1.0e-63
Identity = 127/284 (44.72%), Postives = 168/284 (59.15%), Query Frame = 1


Query: 81  NENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAG 140
           N     V+D+TK GA  DG T+  +AF+ TWI  C + V PA  L+P+  FL GPV FAG
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPV-PATLLVPKGTFLAGPVIFAG 104

Query: 141 PCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFILTGSGVFDGQGVS-------- 200
           PCKS  +T+   GT+ ATT  S Y++ EWF  E V   +LTG+G F G+G +        
Sbjct: 105 PCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCG 164

Query: 201 ----------SIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTD 260
                     S+KF  + +  I+G++S+N+  FH  +    N    N+K+TAP  SPNTD
Sbjct: 165 KKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTD 224

Query: 261 GMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGV 320
           G+HLS +  V+I +S I TGDDCVS+G  + N+ +  V CGPGHGLSVGSLGKY  E+ V
Sbjct: 225 GIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDV 284

Query: 321 YDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVK 347
             + V NCT+    NG RIKT+    P  A  I FE+I+M +VK
Sbjct: 285 SGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVK 324

[Close]

BLAST of Csa7G398220 vs. TAIR10
Match: AT3G14040.1 (AT3G14040.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 218.8 bits (556), Expect = 5.4e-57
Identity = 123/300 (41.00%), Postives = 163/300 (54.33%), Query Frame = 1


Query: 68  GGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIP 127
           GG    +   V   + GG   DV   GAK DG+TDD+ AF   W  AC      +   +P
Sbjct: 51  GGAAASVGAKVTGAKPGGASLDVKASGAKGDGKTDDSAAFAAAWKEAC---AAGSTITVP 110

Query: 128 QDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSS-SEWFSIEDVTGFILTGS-GV 187
           +  +LV  + F GPCK  P+TLE  G  KA   +      + W   E++  F L G+  +
Sbjct: 111 KGEYLVESLEFKGPCKG-PVTLELNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAI 170

Query: 188 FDGQGVSS------------------IKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTA 247
           FDGQG  +                  I+F+ L ++ I+ +TS NS  FH ++  C N T 
Sbjct: 171 FDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITL 230

Query: 248 TNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHG 307
           T++ I AP  S NTDG+H+  S  V +  + I TGDDCVSIG  TEN+I+ NV CGPGHG
Sbjct: 231 TDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHG 290

Query: 308 LSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKI 348
           +S+GSLG+Y  E+ V  V V+ C I N  NG RIKT+    PG+AS I+FEDI M NV +
Sbjct: 291 ISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSL 346

[Close]

BLAST of Csa7G398220 vs. TAIR10
Match: AT4G18180.1 (AT4G18180.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 218.0 bits (554), Expect = 9.1e-57
Identity = 119/288 (41.32%), Postives = 167/288 (57.99%), Query Frame = 1


Query: 80  LNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFA 139
           ++++   + DV   GA+A+   D  +AF+  W  AC+++      +IP+  F VG + F+
Sbjct: 27  VDKSSVFLVDVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFS 86

Query: 140 GPCKSFP-ITLENQGTVKATTDISAY-SSSEWFSIEDVTGFILTGSGVFDGQGV------ 199
           GPC +   +T+     VKA+TD+S Y S   W     + G  LTG G FDGQG       
Sbjct: 87  GPCTNVSNLTVR----VKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFN 146

Query: 200 ------------SSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSP 259
                       +S+KF  +N T++  ++S+NS  FH ++  C +F  T + ITAP +SP
Sbjct: 147 NCTSDSNCKLLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSP 206

Query: 260 NTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKE 319
           NTDG+H+  S  V  S S I TGDDCVSIG     I IT++ CGPGHG+SVGSLG+Y  E
Sbjct: 207 NTDGIHIERSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNE 266

Query: 320 KGVYDVLVKNCTIFNATNGARIKTFASPIPGL--ASRIVFEDIVMYNV 346
           K V  ++VK+C I   TNG RIKT+A+  PGL  A+ + FE+I+M NV
Sbjct: 267 KDVNGLVVKDCKISGTTNGIRIKTWANS-PGLSAATNMTFENIIMNNV 309

[Close]

BLAST of Csa7G398220 vs. TAIR10
Match: AT3G07850.1 (AT3G07850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 210.7 bits (535), Expect = 1.5e-54
Identity = 120/300 (40.00%), Postives = 162/300 (54.00%), Query Frame = 1


Query: 68  GGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIP 127
           GG    +   V   + G  V DV   GAK D +TDD+ AF   W  AC      +   +P
Sbjct: 51  GGAAASVGAKVSGAKPGAAV-DVKASGAKGDSKTDDSAAFAAAWKEAC---AAGSTITVP 110

Query: 128 QDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSS-SEWFSIEDVTGFILTGS-GV 187
           +  ++V  + F GPCK  P+TLE  G  KA   +      + W   E++  F L G+  +
Sbjct: 111 KGEYMVESLEFKGPCKG-PVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAI 170

Query: 188 FDGQGVSS------------------IKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTA 247
           FDGQG  +                  I+F+ L ++ I+ +TS NS  FH ++  C N T 
Sbjct: 171 FDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITL 230

Query: 248 TNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHG 307
           +++ I AP  S NTDG+H+  S  V +  + I TGDDCVSIG  TEN+I+ NV CGPGHG
Sbjct: 231 SDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHG 290

Query: 308 LSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKI 348
           +S+GSLG+Y  E+ V  V V+ C I N  NG RIKT+    PG+AS I+FEDI M NV +
Sbjct: 291 ISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSL 345

[Close]

BLAST of Csa7G398220 vs. TAIR10
Match: AT5G44840.1 (AT5G44840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 204.5 bits (519), Expect = 1.0e-52
Identity = 109/269 (40.52%), Postives = 158/269 (58.74%), Query Frame = 1


Query: 85  GLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFLIPQD-IFLVGPVTFAGPCK 144
           G ++DV + GA  +G TDD++AF+  W A C +      F+IP +  FL+ P+TF GPCK
Sbjct: 21  GQIYDVLEFGADGNGITDDSKAFVKAWSAMCGSGGNSKTFIIPSNKTFLLQPLTFQGPCK 80

Query: 145 SFPITLENQGTVKATTDISAYSSSE---WFSIEDVTGFILTGSGVFDGQGVS---SIKFS 204
           S  + ++  G + A  + +A+S  +   W S +++ G  + GSG   G+G S    + F 
Sbjct: 81  SPSVQVKFDGKIVAPINKAAWSDYKLFRWVSFKEIIGLTVNGSGTIHGRGSSFWKQLHFQ 140

Query: 205 RLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNS 264
           R N  +I G+TS NS   H S+  C     T +K+ APH+SPNTDG+++S S  V I ++
Sbjct: 141 RCNDLMITGITSFNSPKNHISISECKRVKITKIKLVAPHDSPNTDGINISESSDVDIYDT 200

Query: 265 IIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATN 324
           +IGTGDDCV+I   + NI I  + CGPGHG+SVGS+G+  +E  V +V V NCT     N
Sbjct: 201 VIGTGDDCVAINSGSMNINIARMNCGPGHGISVGSVGRDGEESIVENVQVTNCTFIRTDN 260

Query: 325 GARIKTFASPIPGLASRIVFEDIVMYNVK 347
           GARIKT+ +   G A  I+F+ +     K
Sbjct: 261 GARIKTWPNG-KGYAKNILFKSLTFRETK 288

[Close]

BLAST of Csa7G398220 vs. NCBI nr
Match: gi|700189727|gb|KGN44960.1| (hypothetical protein Csa_7G398220 [Cucumis sativus])

HSP 1 Score: 705.7 bits (1820), Expect = 4.1e-200
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 1


Query: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF 60
           MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF
Sbjct: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF 60

Query: 61  STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120
           STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG
Sbjct: 61  STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120

Query: 121 PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL 180
           PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL
Sbjct: 121 PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL 180

Query: 181 TGSGVFDGQGVSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPN 240
           TGSGVFDGQGVSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPN
Sbjct: 181 TGSGVFDGQGVSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPN 240

Query: 241 TDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEK 300
           TDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEK
Sbjct: 241 TDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEK 300

Query: 301 GVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKIL 349
           GVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKIL
Sbjct: 301 GVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVMYNVKIL 348

[Close]

BLAST of Csa7G398220 vs. NCBI nr
Match: gi|778708423|ref|XP_011656188.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 592.8 bits (1527), Expect = 3.9e-166
Identity = 300/364 (82.42%), Postives = 317/364 (87.09%), Query Frame = 1


Query: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF 60
           MTRN SLIQI+LL FAW+ CA    IF D   TQ LL GIVP  N+QFLRPAEAP HLGF
Sbjct: 1   MTRNLSLIQIILLEFAWQSCA---VIFNDITGTQNLLKGIVPTTNKQFLRPAEAPRHLGF 60

Query: 61  STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120
           +TLP+IGG TVNDI  NVDLNENGG VFDVTKHGAKADG+TDDAQAFMTTWIAACRNTVG
Sbjct: 61  NTLPDIGG-TVNDINANVDLNENGGSVFDVTKHGAKADGKTDDAQAFMTTWIAACRNTVG 120

Query: 121 PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL 180
           PAKFLIPQ  FLVGPVTFAGPCKS+PITLENQGTVKATTDISAYSS EWFS+ED+TGFIL
Sbjct: 121 PAKFLIPQGTFLVGPVTFAGPCKSYPITLENQGTVKATTDISAYSSPEWFSLEDITGFIL 180

Query: 181 TGSGVFDGQGVS------------------SIKFSRLNHTIIDGLTSINSMGFHTSVFYC 240
           TGSGVFDGQG+S                  SIKF+RLNHTI+DGLTSINSMGFHTSVFYC
Sbjct: 181 TGSGVFDGQGLSVWPYNDCKKNNLCQLLPISIKFTRLNHTIVDGLTSINSMGFHTSVFYC 240

Query: 241 YNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTC 300
           YNFTATNMKI APHNSPNTDGMHLSTSKLVTI+NSIIGTGDDCVSIGHSTENI +TNVTC
Sbjct: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSIIGTGDDCVSIGHSTENITVTNVTC 300

Query: 301 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVM 347
           GPGHGL   SLGKYSKEKGVYDVLVKNCTIFNATNGARIKT+ASP+ GLASRI+FEDIVM
Sbjct: 301 GPGHGL---SLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPVSGLASRIIFEDIVM 357

[Close]

BLAST of Csa7G398220 vs. NCBI nr
Match: gi|700195561|gb|KGN50738.1| (hypothetical protein Csa_5G222970 [Cucumis sativus])

HSP 1 Score: 556.2 bits (1432), Expect = 4.0e-155
Identity = 278/328 (84.76%), Postives = 293/328 (89.33%), Query Frame = 1


Query: 37  LNGIVPKVNEQFLRPAEAPWHLGFSTLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAK 96
           L GIVP  N+QFLRPAEAP HLGF+TLP+IGG TVNDI  NVDLNENGG VFDVTKHGAK
Sbjct: 6   LYGIVPTTNKQFLRPAEAPRHLGFNTLPDIGG-TVNDINANVDLNENGGSVFDVTKHGAK 65

Query: 97  ADGETDDAQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVK 156
           ADG+TDDAQAFMTTWIAACRNTVGPAKFLIPQ  FLVGPVTFAGPCKS+PITLENQGTVK
Sbjct: 66  ADGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSYPITLENQGTVK 125

Query: 157 ATTDISAYSSSEWFSIEDVTGFILTGSGVFDGQGVS------------------SIKFSR 216
           ATTDISAYSS EWFS+ED+TGFILTGSGVFDGQG+S                  SIKF+R
Sbjct: 126 ATTDISAYSSPEWFSLEDITGFILTGSGVFDGQGLSVWPYNDCKKNNLCQLLPISIKFTR 185

Query: 217 LNHTIIDGLTSINSMGFHTSVFYCYNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNSI 276
           LNHTI+DGLTSINSMGFHTSVFYCYNFTATNMKI APHNSPNTDGMHLSTSKLVTI+NSI
Sbjct: 186 LNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSI 245

Query: 277 IGTGDDCVSIGHSTENIIITNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNG 336
           IGTGDDCVSIGHSTENI +TNVTCGPGHGL   SLGKYSKEKGVYDVLVKNCTIFNATNG
Sbjct: 246 IGTGDDCVSIGHSTENITVTNVTCGPGHGL---SLGKYSKEKGVYDVLVKNCTIFNATNG 305

Query: 337 ARIKTFASPIPGLASRIVFEDIVMYNVK 347
           ARIKT+ASP+ GLASRI+FEDIVMYNVK
Sbjct: 306 ARIKTWASPVSGLASRIIFEDIVMYNVK 329

[Close]

BLAST of Csa7G398220 vs. NCBI nr
Match: gi|659101498|ref|XP_008451635.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 510.4 bits (1313), Expect = 2.5e-141
Identity = 260/364 (71.43%), Postives = 286/364 (78.57%), Query Frame = 1


Query: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF 60
           +TR+  L QILL VFAW+CC + +AI  D  N   L   IV  +N+Q   P  AP  LG 
Sbjct: 3   ITRDIGLFQILLWVFAWQCCDKVSAITNDIAN---LNYEIVSTINQQLPSPVAAPGPLGI 62

Query: 61  STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120
            TLP++     +DI  N DLN NGG VF VTKHGAKADG+TDDAQAF+TTWI ACRNTVG
Sbjct: 63  ETLPDLDNMVNDDINTNNDLNGNGGSVFHVTKHGAKADGKTDDAQAFVTTWIEACRNTVG 122

Query: 121 PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL 180
           PAK LIP+  +LVGPVT AGPCKSFPITLENQGTVKATTDIS YSS EWFSIED+TGFIL
Sbjct: 123 PAKILIPEGTYLVGPVTLAGPCKSFPITLENQGTVKATTDISQYSSPEWFSIEDITGFIL 182

Query: 181 TGSGVFDGQGVS------------------SIKFSRLNHTIIDGLTSINSMGFHTSVFYC 240
           TGSGVFDGQGV+                  SIKFSRLN TI+D LTS+NS GFH S+F C
Sbjct: 183 TGSGVFDGQGVAAWPYNDCKNNNICQLLPISIKFSRLNDTIVDRLTSLNSKGFHMSLFSC 242

Query: 241 YNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTC 300
           YNFTATN+ I APH+SPNTDG+HLSTSKLV I+NSIIGTGDDCVSIGHSTE I +TNVTC
Sbjct: 243 YNFTATNINIIAPHDSPNTDGIHLSTSKLVNIANSIIGTGDDCVSIGHSTEKITVTNVTC 302

Query: 301 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVM 347
           GPGHGLSVGSLGKY +EK VYDVLVKNCTIFNATNGARIKTFASPI G AS I+FEDIVM
Sbjct: 303 GPGHGLSVGSLGKYPEEKDVYDVLVKNCTIFNATNGARIKTFASPISGSASGIIFEDIVM 362

[Close]

BLAST of Csa7G398220 vs. NCBI nr
Match: gi|449462487|ref|XP_004148972.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 494.2 bits (1271), Expect = 1.9e-136
Identity = 255/366 (69.67%), Postives = 284/366 (77.60%), Query Frame = 1


Query: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFL-RPAEAPWHLG 60
           ++R  SL QILLLVFAW+C  + AAI  D I    L N I+  +N+Q +  P   P  LG
Sbjct: 3   ISRILSLFQILLLVFAWQCYDKVAAIITDDIAN--LNNEIISTINQQLIPSPTATPGPLG 62

Query: 61  FSTLPNIGGGTVNDIK-VNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNT 120
             TLP++   TVND    N DLNENG  VFDVTKHGAKADG+TDDAQAF TTWIAACRNT
Sbjct: 63  METLPDLDD-TVNDSNNTNNDLNENGESVFDVTKHGAKADGKTDDAQAFETTWIAACRNT 122

Query: 121 VGPAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGF 180
           VGP K LIP+  +LVGP+T AGPCKSFPIT+ENQG VKATTD+S YSS EWFSIED+TGF
Sbjct: 123 VGPVKILIPKGTYLVGPMTLAGPCKSFPITIENQGIVKATTDLSEYSSPEWFSIEDITGF 182

Query: 181 ILTGSGVFDGQGVS------------------SIKFSRLNHTIIDGLTSINSMGFHTSVF 240
           ILTGSGVFDGQG +                  SIKFS LNHTI+DG+TS+NS GFH S+F
Sbjct: 183 ILTGSGVFDGQGTAVWAYNDCKSNKDCQLLPISIKFSGLNHTIVDGITSLNSKGFHMSLF 242

Query: 241 YCYNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNV 300
            CYNFT TN+ I AP  SPNTDG+HLSTS+LV I NSIIGTGDDCVSIGHST  I +TNV
Sbjct: 243 NCYNFTITNVNIIAPDESPNTDGIHLSTSELVNIMNSIIGTGDDCVSIGHSTVKITVTNV 302

Query: 301 TCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDI 347
           TCGPGHGLSVGSLGKYS+EK VYDVLVKNCTIFNATNGARIKTFASPI GLAS I+FEDI
Sbjct: 303 TCGPGHGLSVGSLGKYSREKDVYDVLVKNCTIFNATNGARIKTFASPISGLASGIIFEDI 362

The following BLAST results are available for this feature:

BLAST of Csa7G398220 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR1_ARATH	1.8e-62	44.72	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana GN=PGA3 PE=2 SV=1	[more]
PGLR_GOSHI	1.6e-58	44.16	Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1	[more]
PGLR_GOSBA	3.5e-58	43.80	Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1	[more]
PGLR2_PLAAC	2.3e-57	40.99	Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1	[more]
PGLR3_MAIZE	1.9e-56	42.18	Exopolygalacturonase OS=Zea mays GN=PG2C PE=2 SV=1	[more]

BLAST of Csa7G398220 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K5I2_CUCSA	2.9e-200	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G398220 PE=3 SV=1	[more]
A0A0A0KMA6_CUCSA	2.8e-155	84.76	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G222970 PE=3 SV=1	[more]
A0A0A0K5G0_CUCSA	1.3e-136	69.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375750 PE=3 SV=1	[more]
A0A0A0KAZ2_CUCSA	1.4e-85	85.42	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G398720 PE=3 SV=1	[more]
W9QFJ0_9ROSA	1.1e-82	53.18	Exopolygalacturonase clone GBGE184 OS=Morus notabilis GN=L484_011125 PE=3 SV=1	[more]

BLAST of Csa7G398220 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G02790.1	1.0e-63	44.72	polygalacturonase 4	[more]
AT3G14040.1	5.4e-57	41.00	Pectin lyase-like superfamily protein	[more]
AT4G18180.1	9.1e-57	41.32	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	1.5e-54	40.00	Pectin lyase-like superfamily protein	[more]
AT5G44840.1	1.0e-52	40.52	Pectin lyase-like superfamily protein	[more]

BLAST of Csa7G398220 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700189727\|gb\|KGN44960.1\|	4.1e-200	100.00	hypothetical protein Csa_7G398220 [Cucumis sativus]	[more]
gi\|778708423\|ref\|XP_011656188.1\|	3.9e-166	82.42	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
gi\|700195561\|gb\|KGN50738.1\|	4.0e-155	84.76	hypothetical protein Csa_5G222970 [Cucumis sativus]	[more]
gi\|659101498\|ref\|XP_008451635.1\|	2.5e-141	71.43	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
gi\|449462487\|ref\|XP_004148972.1\|	1.9e-136	69.67	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000743	Glyco_hydro_28
IPR006626	PbH1
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071555	cell wall organization
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa7G398220.1	Csa7G398220.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 119..346 score: 1.1
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 279..292 scor
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 272..292 score: 5900.0coord: 302..323 score: 10.0coord: 222..248 score: 6900.0coord: 249..270 score: 5
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 86..346 score: 1.3
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 86..346 score: 6.5
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 23..346 score: 3.3E
None	No IPR available	PANTHER	PTHR31375:SF16	SUBFAMILY NOT NAMED	coord: 23..346 score: 3.3E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa7G398220	MELO3C015981	Melon (DHL92) v3.5.1	cumeB532
Csa7G398220	ClCG09G005740	Watermelon (Charleston Gray)	cuwcgB521
Csa7G398220	MELO3C015981.2	Melon (DHL92) v3.6.1	cumedB511
Csa7G398220	CmoCh17G010410	Cucurbita moschata (Rifu)	cmocuB378
Csa7G398220	CsGy7G016090	Cucumber (Gy14) v2	cgybcuB323

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa7G398220	Cucurbita moschata (Rifu)	cmocuB900
Csa7G398220	Watermelon (Charleston Gray)	cuwcgB526
Csa7G398220	Watermelon (Charleston Gray)	cuwcgB531
Csa7G398220	Watermelon (97103) v1	cuwmB560
Csa7G398220	Watermelon (97103) v1	cuwmB568
Csa7G398220	Watermelon (97103) v1	cuwmB588
Csa7G398220	Watermelon (97103) v1	cuwmB574
Csa7G398220	Cucurbita pepo (Zucchini)	cpecuB161
Csa7G398220	Cucurbita pepo (Zucchini)	cpecuB343
Csa7G398220	Bottle gourd (USVL1VR-Ls)	culsiB503
Csa7G398220	Bottle gourd (USVL1VR-Ls)	culsiB516
Csa7G398220	Silver-seed gourd	carcuB0119
Csa7G398220	Silver-seed gourd	carcuB0594
Csa7G398220	Silver-seed gourd	carcuB0833
Csa7G398220	Watermelon (97103) v2	cuwmbB517
Csa7G398220	Watermelon (97103) v2	cuwmbB539
Csa7G398220	Watermelon (97103) v2	cuwmbB565
Csa7G398220	Watermelon (97103) v2	cuwmbB572
Csa7G398220	Wax gourd	cuwgoB650
Csa7G398220	Wax gourd	cuwgoB657
Csa7G398220	Wax gourd	cuwgoB691
Csa7G398220	Cucumber (Chinese Long) v2	cucuB149
Csa7G398220	Cucumber (Gy14) v1	cgycuB312
Csa7G398220	Cucurbita maxima (Rimu)	cmacuB388
Csa7G398220	Cucurbita maxima (Rimu)	cmacuB914