Csa7G074940 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa7G074940
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
Description2-oxoglutarate-dependent dioxygenase; contains IPR026992 (Non-haem dioxygenase N-terminal domain), IPR027443 (Isopenicillin N synthase-like)
LocationChr7 : 5167256 .. 5167792 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGACATCTTCATGAGTTTGATCAGGCTTTCATCCAAGCTCCCGAGCACCGCCCCAAACTCGCACTCTCCCATGCTGATCGAATCCCCACCATCGACCTCTCTCCAATCTTCAACCACTCACTACCCGGCAGCGATGTCCCCCACGATTTGGTCCACCAAATTGCCTCTGCTTGCACGGAGTGGGGATTCTTTCTGGTGGTTAACCACGGCGTCCCTCCCGAGAAGCGGCACCGTATAGAGGCCGCTGCCCGGGAATTTTTCGGCCAAAGTTTGGAGGAGAAGAGGAAAGTGAGGAGGAGCCAAGGGTTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAATGTCAGAGATTGGAAGGAGGTCTTTGATTTCATGGTGGAGGATCCCACCATTGTTCCGGCGTCGCCGGAGTCTGATGATGACGAGTTGACTCAGTGGACTAATAAATGGCCGGAATACCCACCGGAATTCAGGTCATCTCACTATCCAATTATTTTGTTCAATAAGTTATTATACGGAGAATTGGATTGA

mRNA sequence

ATGGGACATCTTCATGAGTTTGATCAGGCTTTCATCCAAGCTCCCGAGCACCGCCCCAAACTCGCACTCTCCCATGCTGATCGAATCCCCACCATCGACCTCTCTCCAATCTTCAACCACTCACTACCCGGCAGCGATGTCCCCCACGATTTGGTCCACCAAATTGCCTCTGCTTGCACGGAGTGGGGATTCTTTCTGGTGGTTAACCACGGCGTCCCTCCCGAGAAGCGGCACCGTATAGAGGCCGCTGCCCGGGAATTTTTCGGCCAAAGTTTGGAGGAGAAGAGGAAAGTGAGGAGGAGCCAAGGGTTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAATGTCAGAGATTGGAAGGAGGTCTTTGATTTCATGGTGGAGGATCCCACCATTGTTCCGGCGTCGCCGGAGTCTGATGATGACGAGTTGACTCAGTGGACTAATAAATGGCCGGAATACCCACCGGAATTCAGGTCATCTCACTATCCAATTATTTTGTTCAATAAGTTATTATACGGAGAATTGGATTGA

Coding sequence (CDS)

ATGGGACATCTTCATGAGTTTGATCAGGCTTTCATCCAAGCTCCCGAGCACCGCCCCAAACTCGCACTCTCCCATGCTGATCGAATCCCCACCATCGACCTCTCTCCAATCTTCAACCACTCACTACCCGGCAGCGATGTCCCCCACGATTTGGTCCACCAAATTGCCTCTGCTTGCACGGAGTGGGGATTCTTTCTGGTGGTTAACCACGGCGTCCCTCCCGAGAAGCGGCACCGTATAGAGGCCGCTGCCCGGGAATTTTTCGGCCAAAGTTTGGAGGAGAAGAGGAAAGTGAGGAGGAGCCAAGGGTTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAATGTCAGAGATTGGAAGGAGGTCTTTGATTTCATGGTGGAGGATCCCACCATTGTTCCGGCGTCGCCGGAGTCTGATGATGACGAGTTGACTCAGTGGACTAATAAATGGCCGGAATACCCACCGGAATTCAGGTCATCTCACTATCCAATTATTTTGTTCAATAAGTTATTATACGGAGAATTGGATTGA

Protein sequence

MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDWKEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSSHYPIILFNKLLYGELD*
Homology
[Close]
BLAST of Csa7G074940 vs. Swiss-Prot
Match: LDOX_PERFR (Leucoanthocyanidin dioxygenase OS=Perilla frutescens GN=ANS PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.0e-09
Identity = 45/139 (32.37%), Postives = 65/139 (46.76%), Query Frame = 1 

Query: 24  SHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAA 83
           S   ++PTIDL  + +    G    H+   ++  A T+WG   ++NHG+P E   R++AA
Sbjct: 48  SEGPQLPTIDLEEMDSRDEEGRKKCHE---ELKKAATDWGVMHLINHGIPEELIDRVKAA 107

Query: 84  AREFFGQSLEEKRKVRRSQ--GLVTGYFDTELTKNVR---DWKEVFDFMVEDPTIVPASP 143
            +EFF   +EEK      Q  G V GY  ++L  N     +W++ F   V         P
Sbjct: 108 GKEFFELPVEEKEAYANDQAAGNVQGY-GSKLANNASGQLEWEDYFFHCV--------YP 167

Query: 144 ESDDDELTQWTNKWPEYPP 158
           E   D L+ W  K P+Y P
Sbjct: 168 EHKTD-LSIWPTKPPDYIP 173

[Close]
BLAST of Csa7G074940 vs. Swiss-Prot
Match: DMR6_ARATH (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 7.6e-09
Identity = 43/154 (27.92%), Postives = 66/154 (42.86%), Query Frame = 1 

Query: 11  FIQAPEHRPKLA-LSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACTEWGFFLVVN 70
           +++    RP+L+ +S  +  P IDLS         S     L+ QI  AC  +GFF V+N
Sbjct: 19  YVRPISDRPRLSEVSQLEDFPLIDLS---------STDRSFLIQQIHQACARFGFFQVIN 78

Query: 71  HGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELT---KNVRDWKEVFDF 130
           HGV  +    + + AREFF  S+EEK K+       T    T      + V +W++    
Sbjct: 79  HGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLRL 138

Query: 131 MVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 161
                             + ++ N+WP  PP F+
Sbjct: 139 HCY--------------PIHKYVNEWPSNPPSFK 149

[Close]
BLAST of Csa7G074940 vs. Swiss-Prot
Match: F6H2_ARATH (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-08
Identity = 40/130 (30.77%), Postives = 58/130 (44.62%), Query Frame = 1 

Query: 27  DRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAARE 86
           + IP ID+S +   S+  +         +  A  EWGFF V+NHGV  E    ++ A   
Sbjct: 60  ESIPVIDISNLDEKSVSKA---------VCDAAEEWGFFQVINHGVSMEVLENMKTATHR 119

Query: 87  FFGQSLEEKRKVRRSQGLVTGY-FDTELTKNVR---DWKEVFD-FMVEDPTIVPASPESD 146
           FFG  +EEKRK  R + L T   F T  + +     +WK+    F V +       P+S 
Sbjct: 120 FFGLPVEEKRKFSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQLWPDSC 179

Query: 147 DDELTQWTNK 152
             E  ++ N+
Sbjct: 180 RSETLEYMNE 180

[Close]
BLAST of Csa7G074940 vs. Swiss-Prot
Match: FL3H_MALDO (Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-08
Identity = 39/129 (30.23%), Postives = 63/129 (48.84%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSH-ADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASAC 60
           + H     Q F++  + RPK+A +  ++ IP I L+ I    + G     ++  +I +AC
Sbjct: 10  IAHEKTLQQKFVRDEDERPKVAYNDFSNEIPIISLAGI--DEVEGRR--GEICKKIVAAC 69

Query: 61  TEWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKN--V 120
            +WG F +V+HGV  E    +   AREFF    EEK +   S G   G+  +   +   V
Sbjct: 70  EDWGIFQIVDHGVDAELISEMTGLAREFFALPSEEKLRFDMSGGKKGGFIVSSHLQGEAV 129

Query: 121 RDWKEVFDF 127
           +DW+E+  +
Sbjct: 130 QDWREIVTY 134

[Close]
BLAST of Csa7G074940 vs. Swiss-Prot
Match: F6H1_ARATH (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-08
Identity = 38/126 (30.16%), Postives = 57/126 (45.24%), Query Frame = 1 

Query: 31  TIDLSPIFNHSLPGSDVPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFGQ 90
           T +  P+ + S P  D    +   +  A  +WGFF V+NHGVP E    ++AA  +FF  
Sbjct: 58  TDEAIPVIDMSNPDED---RVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 117

Query: 91  SLEEKRKVRRSQGLVT----GYFDTELTKNVRDWKEVFD-FMVEDPTIVPASPESDDDEL 150
            +EEKRK  +   L T    G   + L +   +WK+    F V +       P+   +E 
Sbjct: 118 PVEEKRKFTKENSLSTTVRFGTSFSPLAEQALEWKDYLSLFFVSEAEAEQFWPDICRNET 177

Query: 151 TQWTNK 152
            ++ NK
Sbjct: 178 LEYINK 180

[Close]
BLAST of Csa7G074940 vs. TrEMBL
Match: A0A0A0K834_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074940 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 9.1e-102
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60
           MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT
Sbjct: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60

Query: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120
           EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW
Sbjct: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120

Query: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSSHYPIILFNKLLYGELD 179
           KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSSHYPIILFNKLLYGELD
Sbjct: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSSHYPIILFNKLLYGELD 178

[Close]
BLAST of Csa7G074940 vs. TrEMBL
Match: A0A0A0K3A9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074960 PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 4.1e-78
Identity = 145/162 (89.51%), Postives = 151/162 (93.21%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60
           MG L +F    IQAPEHRPKLALSHAD IPTIDLSPIFN SLPGSD PHDLVHQIASACT
Sbjct: 1   MGDLEDF----IQAPEHRPKLALSHADGIPTIDLSPIFN-SLPGSDFPHDLVHQIASACT 60

Query: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120
           EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRS+GLVTGYFD+ELTKNVRDW
Sbjct: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGLVTGYFDSELTKNVRDW 120

Query: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSS 163
           KEVFD +VEDPTIVPASPESDD+ELTQWTN+WPEYPPEFR S
Sbjct: 121 KEVFDLVVEDPTIVPASPESDDEELTQWTNQWPEYPPEFRES 157

[Close]
BLAST of Csa7G074940 vs. TrEMBL
Match: A0A0A0K6P5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074950 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.3e-71
Identity = 131/161 (81.37%), Postives = 144/161 (89.44%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLP-GSDVPHDLVHQIASAC 60
           MGHL EFDQ FIQAP+HRPKLA+S AD IPTIDLSPIFN S P GS+ PHDLV QIASAC
Sbjct: 1   MGHLQEFDQVFIQAPDHRPKLAVSQADGIPTIDLSPIFNDSPPAGSEFPHDLVQQIASAC 60

Query: 61  TEWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRD 120
           TEWGFFLVVNHGVPPEKR R+EAAAREFFGQSLEEKRKVRR++G+ TGYFD ELTKNVRD
Sbjct: 61  TEWGFFLVVNHGVPPEKRRRMEAAAREFFGQSLEEKRKVRRNEGVATGYFDMELTKNVRD 120

Query: 121 WKEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 161
           WKEVFDF+VEDPT++PAS + D+ ELTQ  N+WPEYPPEFR
Sbjct: 121 WKEVFDFVVEDPTLIPASSDPDETELTQLINQWPEYPPEFR 161

[Close]
BLAST of Csa7G074940 vs. TrEMBL
Match: K7ZTB8_CUCSA (Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox2 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.3e-71
Identity = 131/161 (81.37%), Postives = 144/161 (89.44%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLP-GSDVPHDLVHQIASAC 60
           MGHL EFDQ FIQAP+HRPKLA+S AD IPTIDLSPIFN S P GS+ PHDLV QIASAC
Sbjct: 1   MGHLQEFDQVFIQAPDHRPKLAVSQADGIPTIDLSPIFNDSPPAGSEFPHDLVQQIASAC 60

Query: 61  TEWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRD 120
           TEWGFFLVVNHGVPPEKR R+EAAAREFFGQSLEEKRKVRR++G+ TGYFD ELTKNVRD
Sbjct: 61  TEWGFFLVVNHGVPPEKRRRMEAAAREFFGQSLEEKRKVRRNEGVATGYFDMELTKNVRD 120

Query: 121 WKEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 161
           WKEVFDF+VEDPT++PAS + D+ ELTQ  N+WPEYPPEFR
Sbjct: 121 WKEVFDFVVEDPTLIPASSDPDETELTQLINQWPEYPPEFR 161

[Close]
BLAST of Csa7G074940 vs. TrEMBL
Match: M5VMX5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007775mg PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 4.5e-48
Identity = 96/159 (60.38%), Postives = 117/159 (73.58%), Query Frame = 1 

Query: 6   EFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGS----DVPHDLVHQIASACTE 65
           E D AFIQ PEHRPKL+++ A+ IP IDLSPI +   P S         LV +I SAC  
Sbjct: 3   EVDPAFIQDPEHRPKLSITEAEGIPLIDLSPINSILTPDSISELKAIEGLVREIGSACKN 62

Query: 66  WGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDWK 125
           WGFF V+NHGV  +KR +IE AAR+FF Q LEEKRK+RR + +V GY+DTE TKNVRDWK
Sbjct: 63  WGFFQVINHGVALDKREKIETAARKFFAQPLEEKRKIRRDEKIVVGYYDTEHTKNVRDWK 122

Query: 126 EVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 161
           EVFDF+VE+PT+VPAS + +D E T+W N+WPE PPE R
Sbjct: 123 EVFDFVVEEPTLVPASLDPEDKEETEWINQWPENPPELR 161

[Close]
BLAST of Csa7G074940 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 172.2 bits (435), Expect = 3.0e-43
Identity = 87/164 (53.05%), Postives = 105/164 (64.02%), Query Frame = 1 

Query: 6   EFDQAFIQAPEHRPKLALSHA------DRIPTIDLSPIFNHSLPGSDVPHD---LVHQIA 65
           E D+AFIQAPEHRP   L+++      D IPTIDLS +        D  HD   +  +IA
Sbjct: 3   ELDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSL-------EDTHHDKTAIAKEIA 62

Query: 66  SACTEWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKN 125
            AC  WGFF V+NHG+P   RHR+E  A EFF  + EEKRKV+R +    GY D E TKN
Sbjct: 63  EACKRWGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKN 122

Query: 126 VRDWKEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 161
           VRDWKE+FDF ++D TIVPASPE +D EL + TN+WP+ P  FR
Sbjct: 123 VRDWKEIFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFR 159

[Close]
BLAST of Csa7G074940 vs. TAIR10
Match: AT3G19010.1 (AT3G19010.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 135.6 bits (340), Expect = 3.1e-32
Identity = 68/164 (41.46%), Postives = 94/164 (57.32%), Query Frame = 1 

Query: 4   LHEFDQAFIQAPEHRP--KLALSHADRIPTIDLSPIFNHSLPGSDVPHDL---VHQIASA 63
           + + D  +IQAPEHR       +  + IP IDLS +        D P D+   + +I  A
Sbjct: 1   MEDLDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRL--------DDPEDVQNVISEIGDA 60

Query: 64  CTEWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVR 123
           C +WGFF V+NHGVP + R R+E   + FF   +EEK KV+R      GY D E TKNV+
Sbjct: 61  CEKWGFFQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVK 120

Query: 124 DWKEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSS 163
           DWKEVFD   +DP ++P++ + +D+ L    NKWP+ P +FR +
Sbjct: 121 DWKEVFDIYFKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREA 156

[Close]
BLAST of Csa7G074940 vs. TAIR10
Match: AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 68.6 bits (166), Expect = 4.6e-12
Identity = 47/137 (34.31%), Postives = 61/137 (44.53%), Query Frame = 1 

Query: 28  RIPTIDLSPIFNHSLPGSDVPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREF 87
           +IP IDLS +   S P +D     + +++ AC +WGFF V+NHG+  E    IE  A EF
Sbjct: 54  QIPVIDLSKL---SKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEF 113

Query: 88  FGQSLEEKRKVRRSQGLVTGYFDTELTKNVR--DWKEVFDFMVEDPTIVPASPESDDDEL 147
           F   LEEK+K     G V GY    +    +  DW  +F   V  P I   +P+      
Sbjct: 114 FDMPLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQI--RNPK------ 173

Query: 148 TQWTNKWPEYPPEFRSS 163
                 WP  P  F  S
Sbjct: 174 -----LWPSKPARFSES 174

[Close]
BLAST of Csa7G074940 vs. TAIR10
Match: AT2G38240.1 (AT2G38240.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 67.4 bits (163), Expect = 1.0e-11
Identity = 49/155 (31.61%), Postives = 70/155 (45.16%), Query Frame = 1 

Query: 11  FIQAPEHRPKLALSHAD---RIPTIDLSPIFNHSLPGSDVPHDLVHQIASACTEWGFFLV 70
           +++    RP    + +D    IP +D++ ++         P  L   + SAC EWGFF +
Sbjct: 27  YVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGK-------PEGL-RLVRSACEEWGFFQM 86

Query: 71  VNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFD-TELTKNVR-DWKEVFD 130
           VNHGV      R+  A REFF   LEEKRK   S     GY     + K+ + DW + F 
Sbjct: 87  VNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYF- 146

Query: 131 FMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 161
           F+      +P+S  +         +KWP  PP+ R
Sbjct: 147 FL----NYLPSSIRN--------PSKWPSQPPKIR 160

[Close]
BLAST of Csa7G074940 vs. TAIR10
Match: AT5G24530.1 (AT5G24530.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 62.0 bits (149), Expect = 4.3e-10
Identity = 43/154 (27.92%), Postives = 66/154 (42.86%), Query Frame = 1 

Query: 11  FIQAPEHRPKLA-LSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACTEWGFFLVVN 70
           +++    RP+L+ +S  +  P IDLS         S     L+ QI  AC  +GFF V+N
Sbjct: 19  YVRPISDRPRLSEVSQLEDFPLIDLS---------STDRSFLIQQIHQACARFGFFQVIN 78

Query: 71  HGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELT---KNVRDWKEVFDF 130
           HGV  +    + + AREFF  S+EEK K+       T    T      + V +W++    
Sbjct: 79  HGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLRL 138

Query: 131 MVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 161
                             + ++ N+WP  PP F+
Sbjct: 139 HCY--------------PIHKYVNEWPSNPPSFK 149

[Close]
BLAST of Csa7G074940 vs. NCBI nr
Match: gi|700188728|gb|KGN43961.1| (hypothetical protein Csa_7G074940 [Cucumis sativus])

HSP 1 Score: 377.5 bits (968), Expect = 1.3e-101
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60
           MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT
Sbjct: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60

Query: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120
           EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW
Sbjct: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120

Query: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSSHYPIILFNKLLYGELD 179
           KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSSHYPIILFNKLLYGELD
Sbjct: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSSHYPIILFNKLLYGELD 178

[Close]
BLAST of Csa7G074940 vs. NCBI nr
Match: gi|778725225|ref|XP_004137123.2| (PREDICTED: hyoscyamine 6-dioxygenase-like isoform X1 [Cucumis sativus])

HSP 1 Score: 340.9 bits (873), Expect = 1.4e-90
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60
           MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT
Sbjct: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60

Query: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120
           EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW
Sbjct: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120

Query: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 161
           KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR
Sbjct: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFR 160

[Close]
BLAST of Csa7G074940 vs. NCBI nr
Match: gi|778725233|ref|XP_004136997.2| (PREDICTED: gibberellin 20 oxidase 1-like isoform X4 [Cucumis sativus])

HSP 1 Score: 298.9 bits (764), Expect = 5.9e-78
Identity = 145/162 (89.51%), Postives = 151/162 (93.21%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60
           MG L +F    IQAPEHRPKLALSHAD IPTIDLSPIFN SLPGSD PHDLVHQIASACT
Sbjct: 1   MGDLEDF----IQAPEHRPKLALSHADGIPTIDLSPIFN-SLPGSDFPHDLVHQIASACT 60

Query: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120
           EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRS+GLVTGYFD+ELTKNVRDW
Sbjct: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGLVTGYFDSELTKNVRDW 120

Query: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSS 163
           KEVFD +VEDPTIVPASPESDD+ELTQWTN+WPEYPPEFR S
Sbjct: 121 KEVFDLVVEDPTIVPASPESDDEELTQWTNQWPEYPPEFRES 157

[Close]
BLAST of Csa7G074940 vs. NCBI nr
Match: gi|778725230|ref|XP_011658920.1| (PREDICTED: gibberellin 20 oxidase 2-like isoform X3 [Cucumis sativus])

HSP 1 Score: 298.9 bits (764), Expect = 5.9e-78
Identity = 145/162 (89.51%), Postives = 151/162 (93.21%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60
           MG L +F    IQAPEHRPKLALSHAD IPTIDLSPIFN SLPGSD PHDLVHQIASACT
Sbjct: 1   MGDLEDF----IQAPEHRPKLALSHADGIPTIDLSPIFN-SLPGSDFPHDLVHQIASACT 60

Query: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120
           EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRS+GLVTGYFD+ELTKNVRDW
Sbjct: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGLVTGYFDSELTKNVRDW 120

Query: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSS 163
           KEVFD +VEDPTIVPASPESDD+ELTQWTN+WPEYPPEFR S
Sbjct: 121 KEVFDLVVEDPTIVPASPESDDEELTQWTNQWPEYPPEFRES 157

[Close]
BLAST of Csa7G074940 vs. NCBI nr
Match: gi|778725239|ref|XP_011658922.1| (PREDICTED: gibberellin 20 oxidase 2-like isoform X6 [Cucumis sativus])

HSP 1 Score: 298.9 bits (764), Expect = 5.9e-78
Identity = 145/162 (89.51%), Postives = 151/162 (93.21%), Query Frame = 1 

Query: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60
           MG L +F    IQAPEHRPKLALSHAD IPTIDLSPIFN SLPGSD PHDLVHQIASACT
Sbjct: 1   MGDLEDF----IQAPEHRPKLALSHADGIPTIDLSPIFN-SLPGSDFPHDLVHQIASACT 60

Query: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120
           EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRS+GLVTGYFD+ELTKNVRDW
Sbjct: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGLVTGYFDSELTKNVRDW 120

Query: 121 KEVFDFMVEDPTIVPASPESDDDELTQWTNKWPEYPPEFRSS 163
           KEVFD +VEDPTIVPASPESDD+ELTQWTN+WPEYPPEFR S
Sbjct: 121 KEVFDLVVEDPTIVPASPESDDEELTQWTNQWPEYPPEFRES 157

The following BLAST results are available for this feature:
BLAST of Csa7G074940 vs. Swiss-Prot
Match NameE-valueIdentityDescription
LDOX_PERFR2.0e-0932.37Leucoanthocyanidin dioxygenase OS=Perilla frutescens GN=ANS PE=2 SV=1[more]
DMR6_ARATH7.6e-0927.92Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1[more]
F6H2_ARATH1.7e-0830.77Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1[more]
FL3H_MALDO1.7e-0830.23Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica PE=2 SV=1[more]
F6H1_ARATH2.9e-0830.16Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana GN=F6'H1 PE=1 SV=1[more]
BLAST of Csa7G074940 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K834_CUCSA9.1e-102100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074940 PE=4 SV=1[more]
A0A0A0K3A9_CUCSA4.1e-7889.51Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074960 PE=3 SV=1[more]
A0A0A0K6P5_CUCSA1.3e-7181.37Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074950 PE=3 SV=1[more]
K7ZTB8_CUCSA1.3e-7181.37Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox2 PE=2 SV=1[more]
M5VMX5_PRUPE4.5e-4860.38Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007775mg PE=3 SV=1[more]
BLAST of Csa7G074940 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G19000.13.0e-4353.05 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT3G19010.13.1e-3241.46 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT3G21420.14.6e-1234.31 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT2G38240.11.0e-1131.61 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G24530.14.3e-1027.92 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Csa7G074940 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700188728|gb|KGN43961.1|1.3e-101100.00hypothetical protein Csa_7G074940 [Cucumis sativus][more]
gi|778725225|ref|XP_004137123.2|1.4e-90100.00PREDICTED: hyoscyamine 6-dioxygenase-like isoform X1 [Cucumis sativus][more]
gi|778725233|ref|XP_004136997.2|5.9e-7889.51PREDICTED: gibberellin 20 oxidase 1-like isoform X4 [Cucumis sativus][more]
gi|778725230|ref|XP_011658920.1|5.9e-7889.51PREDICTED: gibberellin 20 oxidase 2-like isoform X3 [Cucumis sativus][more]
gi|778725239|ref|XP_011658922.1|5.9e-7889.51PREDICTED: gibberellin 20 oxidase 2-like isoform X6 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR026992DIOX_N
IPR027443IPNS-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016707 gibberellin 3-beta-dioxygenase activity
molecular_function GO:0005506 iron ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa7G074940.1Csa7G074940.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 29..154
score: 5.4
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 4..161
score: 2.7
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 6..160
score: 5.5
NoneNo IPR availablePANTHERPTHR10209:SF98SUBFAMILY NOT NAMEDcoord: 6..160
score: 5.5
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 4..162
score: 9.61

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa7G074940Wax gourdcuwgoB663
Csa7G074940Wax gourdcuwgoB686
Csa7G074940Cucumber (Chinese Long) v2cucuB079
Csa7G074940Cucumber (Chinese Long) v2cucuB083
Csa7G074940Cucumber (Gy14) v1cgycuB322
Csa7G074940Cucurbita maxima (Rimu)cmacuB146
Csa7G074940Cucurbita maxima (Rimu)cmacuB528
Csa7G074940Cucurbita moschata (Rifu)cmocuB137
Csa7G074940Cucurbita moschata (Rifu)cmocuB519
Csa7G074940Melon (DHL92) v3.5.1cumeB530
Csa7G074940Melon (DHL92) v3.5.1cumeB549
Csa7G074940Watermelon (Charleston Gray)cuwcgB538
Csa7G074940Watermelon (Charleston Gray)cuwcgB547
Csa7G074940Watermelon (Charleston Gray)cuwcgB568
Csa7G074940Watermelon (97103) v1cuwmB576
Csa7G074940Watermelon (97103) v1cuwmB595
Csa7G074940Watermelon (97103) v1cuwmB605
Csa7G074940Cucurbita pepo (Zucchini)cpecuB258
Csa7G074940Cucurbita pepo (Zucchini)cpecuB695
Csa7G074940Bottle gourd (USVL1VR-Ls)culsiB492
Csa7G074940Bottle gourd (USVL1VR-Ls)culsiB495
Csa7G074940Bottle gourd (USVL1VR-Ls)culsiB537
Csa7G074940Cucumber (Gy14) v2cgybcuB087
Csa7G074940Cucumber (Gy14) v2cgybcuB091
Csa7G074940Cucumber (Gy14) v2cgybcuB324
Csa7G074940Melon (DHL92) v3.6.1cumedB509
Csa7G074940Melon (DHL92) v3.6.1cumedB526
Csa7G074940Silver-seed gourdcarcuB0233
Csa7G074940Silver-seed gourdcarcuB0827
Csa7G074940Watermelon (97103) v2cuwmbB516
Csa7G074940Watermelon (97103) v2cuwmbB530
Csa7G074940Watermelon (97103) v2cuwmbB554
Csa7G074940Watermelon (97103) v2cuwmbB569