Csa7G018730 (gene) Cucumber (Chinese Long) v2

NameCsa7G018730
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionO-methyltransferase, putative; contains IPR016461 (Caffeate O-methyltransferase (COMT) family)
LocationChr7 : 1076956 .. 1078803 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGTTAAGCACAAAGAATCTTCATCAGAGAAGGAAGAAGATGGCCAAGCCATCATCCAAATGTGGAGATACATATTTAGATTCACTGAAATGGCAGCAATAAAATGTGTAGTAGACCTTAAAATTGCTGACATTATAGAAAGCTATGGAAGCCCCGTGACATTGTCACAATTATCCTCTACTTTAAACTGCTCTTCTTCTCTCCTTCACCGCATCTTGAGATTTCTCATTCATCGTGGAATTTTCAAACAAGAAACCATTGATGAAAACCAAATAGGTTATTCACAAACACCTATGTCTCGCTTGCTTGCAACCAATGTTGAGAACAGCATGGCTCCTTTGCTTCTATTGGAAACAAGTCCAGTGATGCTTGCACCATGGCAACACTTGAGTGGCCATTTAAAGAACAGTGACACTTTGCCATTTGAGATTGCTCATGGGAAGGATTTGTGGAGGTATGCTGAAGCAAATCACGAACATAATTTGCTGTTCAATGAGGCCATGGCTTGTGGTGCTAAGATGATTGTTTCAGCTATAATTGAAGGGTGTGGGGATGTTTTTGATGGAGTTAGATGTTTGGTGGATGTTGGAGGTGGAAATGGAAGCACTTTGAGCATTTTGGTGAAGGCCTATCCATGGATGAAAGGTATTAACTTCGATCTTCCTCATGTTGTGTGTGCTTCACCACAATATGAGAATGTGGAGCATGTTGCTGGTAACATGTTTGATTTTGTTCCAAATGCTGATGTTGCTTTCCTCAAGGTTGGTATATATGCGTGTGTTAATAAATAGCTCCACGTTTTTATGTTTTGTTTCATTGAGTTGAGTACCTTTAGTTTTTGGGTTGGTTTAACGTTTTTTTTTATAATGATTTTCAAATGGTTAAAAGCATTGATCATTTAAAATCTTCGGTGTTTGATTTTACGACTCTCAAATGCAATCATCAAATCACTAATAAAATAGATCTTGAATATTAAAAGTCATGGTTTTGGAGTGATTATGAATGTGACAAAAAGTTTTTAAAAAAAAAATCAAAGAGGTCAGTACAACTTTTACTCTCTATGTTTGAAATATTCATGCTCGTTCAAGGGCTTGAACTTGGAGGCATCAATAACTTTTTATATTAAGTTCACCCAAAGGTTCAAAAGTTGAGACGTTTAAGGCAACATGACCAAGAGACTCCATTGCCCAAAACTACCCTTAAAATTGAGCCTAATTCCTCTAAATTTCTTGTCATTGGTTAAGAGTTGAATTCTTTCAAAATTTTGGGTTTTCTTGTTGAATATTAGTTAAAAGATAGATAAAGAAAGTGAAGAAATTTAGCGGTAGAATAGTTGGAAGGTAATCTAATTTTGAAAAAGTAGATAATTAAGCAAATGTTTGGAAATTGAACTGCAGTGGATTTTACATGATTGGGACGATGAAGAATGTATTAAAATTTTGAAGAAATGCAAGGAATCGATTCCAAAAAGTGGAGGGAAGGTGATAATCATAGAAGCAATTATAGAAGCTGAGAAAGGTGAGAAAAAGAACAAGAAGTTATCAGATGTGGGATTGATGTTTGACCTAGTCATGATGGCCCATACCAACAGAGGCAAAGAAAGAACAGCTCAAGAATGGGCTTTCCTTATCCATCAAGCTGGCTTCACTACACACACCATTACACCCATTCAAGCTATCCAATCACTTATTCAATGTTTCCCTTAAAATCAAAACAACCAATCAATCAAAAAAACTTTGTGTTACTACTTCTTCTATATATGTACTGGTTATAGCATCTATTTCTACTGTCCTACTTTATATTGATAACAATAGACTTGTTGCTGCAAATATCTTCGCATTGGG

mRNA sequence

ATGGAAGTTAAGCACAAAGAATCTTCATCAGAGAAGGAAGAAGATGGCCAAGCCATCATCCAAATGTGGAGATACATATTTAGATTCACTGAAATGGCAGCAATAAAATGTGTAGTAGACCTTAAAATTGCTGACATTATAGAAAGCTATGGAAGCCCCGTGACATTGTCACAATTATCCTCTACTTTAAACTGCTCTTCTTCTCTCCTTCACCGCATCTTGAGATTTCTCATTCATCGTGGAATTTTCAAACAAGAAACCATTGATGAAAACCAAATAGGTTATTCACAAACACCTATGTCTCGCTTGCTTGCAACCAATGTTGAGAACAGCATGGCTCCTTTGCTTCTATTGGAAACAAGTCCAGTGATGCTTGCACCATGGCAACACTTGAGTGGCCATTTAAAGAACAGTGACACTTTGCCATTTGAGATTGCTCATGGGAAGGATTTGTGGAGGTATGCTGAAGCAAATCACGAACATAATTTGCTGTTCAATGAGGCCATGGCTTGTGGTGCTAAGATGATTGTTTCAGCTATAATTGAAGGGTGTGGGGATGTTTTTGATGGAGTTAGATGTTTGGTGGATGTTGGAGGTGGAAATGGAAGCACTTTGAGCATTTTGGTGAAGGCCTATCCATGGATGAAAGGTATTAACTTCGATCTTCCTCATGTTGTGTGTGCTTCACCACAATATGAGAATGTGGAGCATGTTGCTGGTAACATGTTTGATTTTGTTCCAAATGCTGATGTTGCTTTCCTCAAGTGGATTTTACATGATTGGGACGATGAAGAATGTATTAAAATTTTGAAGAAATGCAAGGAATCGATTCCAAAAAGTGGAGGGAAGGTGATAATCATAGAAGCAATTATAGAAGCTGAGAAAGGTGAGAAAAAGAACAAGAAGTTATCAGATGTGGGATTGATGTTTGACCTAGTCATGATGGCCCATACCAACAGAGGCAAAGAAAGAACAGCTCAAGAATGGGCTTTCCTTATCCATCAAGCTGGCTTCACTACACACACCATTACACCCATTCAAGCTATCCAATCACTTATTCAATGTTTCCCTTAA

Coding sequence (CDS)

ATGGAAGTTAAGCACAAAGAATCTTCATCAGAGAAGGAAGAAGATGGCCAAGCCATCATCCAAATGTGGAGATACATATTTAGATTCACTGAAATGGCAGCAATAAAATGTGTAGTAGACCTTAAAATTGCTGACATTATAGAAAGCTATGGAAGCCCCGTGACATTGTCACAATTATCCTCTACTTTAAACTGCTCTTCTTCTCTCCTTCACCGCATCTTGAGATTTCTCATTCATCGTGGAATTTTCAAACAAGAAACCATTGATGAAAACCAAATAGGTTATTCACAAACACCTATGTCTCGCTTGCTTGCAACCAATGTTGAGAACAGCATGGCTCCTTTGCTTCTATTGGAAACAAGTCCAGTGATGCTTGCACCATGGCAACACTTGAGTGGCCATTTAAAGAACAGTGACACTTTGCCATTTGAGATTGCTCATGGGAAGGATTTGTGGAGGTATGCTGAAGCAAATCACGAACATAATTTGCTGTTCAATGAGGCCATGGCTTGTGGTGCTAAGATGATTGTTTCAGCTATAATTGAAGGGTGTGGGGATGTTTTTGATGGAGTTAGATGTTTGGTGGATGTTGGAGGTGGAAATGGAAGCACTTTGAGCATTTTGGTGAAGGCCTATCCATGGATGAAAGGTATTAACTTCGATCTTCCTCATGTTGTGTGTGCTTCACCACAATATGAGAATGTGGAGCATGTTGCTGGTAACATGTTTGATTTTGTTCCAAATGCTGATGTTGCTTTCCTCAAGTGGATTTTACATGATTGGGACGATGAAGAATGTATTAAAATTTTGAAGAAATGCAAGGAATCGATTCCAAAAAGTGGAGGGAAGGTGATAATCATAGAAGCAATTATAGAAGCTGAGAAAGGTGAGAAAAAGAACAAGAAGTTATCAGATGTGGGATTGATGTTTGACCTAGTCATGATGGCCCATACCAACAGAGGCAAAGAAAGAACAGCTCAAGAATGGGCTTTCCTTATCCATCAAGCTGGCTTCACTACACACACCATTACACCCATTCAAGCTATCCAATCACTTATTCAATGTTTCCCTTAA

Protein sequence

MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAIIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKNKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP*
BLAST of Csa7G018730 vs. Swiss-Prot
Match: 4OMT2_PAPSO (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.2e-76
Identity = 148/356 (41.57%), Postives = 218/356 (61.24%), Query Frame = 1

Query: 6   KESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTL-- 65
           K +++ +E   +   Q+W  I+ F +   ++C V++ IADII++    +TL+QL++ L  
Sbjct: 7   KPAAATQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPI 66

Query: 66  -NCSSSLLHRILRFLIHRGIFKQETIDEN-QIGYSQTPMSRLLATNVENSMAPLLLLETS 125
            N SS  L+R++R+L+H  I +QET +   +  YS  P+  LL  + E SM P++L  T 
Sbjct: 67  TNVSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQ 126

Query: 126 PVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAII 185
              +  W  +   L N  T  FE   G D+W+Y E N + + LFNE MA   +++   +I
Sbjct: 127 KDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLI 186

Query: 186 EGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGN 245
           E C D F G+  LVD+GGGNG+T+  + +A+P +K   +DLPHVV  S    N+E V G+
Sbjct: 187 EDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGD 246

Query: 246 MFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKNK 305
           MF  VP+A    LK ILHDW DEEC+ ILKKCKE+IPK  GKVII++  +E    E+ N 
Sbjct: 247 MFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALE----EESNH 306

Query: 306 KLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
           +L+   L+ D+ M+ +T  G+ERTA +W  L+ +AGF +H I PI+AIQS+I+ FP
Sbjct: 307 ELTKTRLILDIDMLVNTG-GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357

BLAST of Csa7G018730 vs. Swiss-Prot
Match: 6OMT_COPJA ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 5.5e-75
Identity = 147/360 (40.83%), Postives = 216/360 (60.00%), Query Frame = 1

Query: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60
           MEVK    SS+ +        +W +I+ F E   +KC V L +A+II + G+ +TLS+LS
Sbjct: 1   MEVKKDNLSSQAK--------LWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELS 60

Query: 61  STLNC---SSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLL 120
           S L     +   L+R++R+L+H  +F + +ID  ++ Y   P ++ L    +  M   +L
Sbjct: 61  SRLPSQPVNEDALYRVMRYLVHMKLFTKASID-GELRYGLAPPAKYLVKGWDKCMVGSIL 120

Query: 121 LETSPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIV 180
             T    +APW +L   L       FE A G ++W Y   + E N LFNEAMA  +++I+
Sbjct: 121 AITDKDFMAPWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIM 180

Query: 181 SAIIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEH 240
           SA+++ CG++F+G+  LVDVGGG G+ +  +  A+P +K   +DLPHV+  SP Y  V  
Sbjct: 181 SALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHC 240

Query: 241 VAGNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGE 300
           VAG+MF F+P AD   +K ILHDWDD+ECI+ILK+CKE++P  GGKVII++ ++  +   
Sbjct: 241 VAGDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEH 300

Query: 301 KKNKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
              K    + L  DL MM +T  GKERT +EW  LIH AG+  H IT I A+QS+I+ +P
Sbjct: 301 PYTK----MRLTLDLDMMLNTG-GKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346

BLAST of Csa7G018730 vs. Swiss-Prot
Match: 7OMT_PAPSO ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum GN=7OMT PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.3e-73
Identity = 146/359 (40.67%), Postives = 220/359 (61.28%), Query Frame = 1

Query: 9   SSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNCSS- 68
           ++E+   GQA  ++W ++F F +  A+KC V+L I DII S+G PVT+S++  +L  ++ 
Sbjct: 3   TAEERLKGQA--EIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTP 62

Query: 69  ------SLLHRILRFLIHRGIFKQETIDE-NQIGYSQTPMSRLLATNVENSMAPLLLLET 128
                   L RI+R L+H+ +F  E   E NQ+ Y+ T  S+ L  + + +++PL+L ET
Sbjct: 63  SSSPNIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWET 122

Query: 129 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI 188
           +P++L PWQ+L G      + PFE AHG ++W  A A+ + N   N AM C    I++ +
Sbjct: 123 NPILLKPWQYL-GKCAQEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEM 182

Query: 189 IEGCGDVFDGVR-CLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVA 248
           +    D F G+   LVDVGGG GS ++ +VKA+P ++GINFDLPHVV  + ++  V+HV 
Sbjct: 183 LLEYKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVG 242

Query: 249 GNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESI-PKSGGKVIIIEAIIEAEKGEK 308
           G+MF  +P AD   +KWILHDW DE+C  ILK C  +I  K  GKVII++ ++  +  + 
Sbjct: 243 GDMFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDL 302

Query: 309 KNKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
            +K    +GL+FD++MMAHT  GKERT  EW  L++ AGF  + +    A   +I+ FP
Sbjct: 303 FDK----MGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

BLAST of Csa7G018730 vs. Swiss-Prot
Match: Q8OMT_MENPI (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita GN=OMT2 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.4e-72
Identity = 145/363 (39.94%), Postives = 213/363 (58.68%), Query Frame = 1

Query: 10  SEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNCSSSL 69
           S K+E  +A   +W +I+ +    ++KC + L I D I  +G+P+TLSQL+  LN + + 
Sbjct: 8   SSKQELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNINKAK 67

Query: 70  LH---RILRFLIHRGIFKQ---------ETIDENQIGYSQTPMSRLLATNVENSMAPLLL 129
            H   R++R L+H G F +         E  +E +  YS TP SRLL  +   S+AP  L
Sbjct: 68  SHGLFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPFAL 127

Query: 130 LETSPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIV 189
             + PV    W HLS   +N     F+  +G     YA A+   N+LFNEAMAC A  + 
Sbjct: 128 AMSDPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGFVN 187

Query: 190 SAIIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEH 249
           S +   C ++FDG+  +VDVGGG G+T   +  A+P M+    DLP+VV      EN+  
Sbjct: 188 SILTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSENLSF 247

Query: 250 VAGNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGG---KVIIIEAIIEAE 309
           V+G+MFDF+P+AD  F+K+ILHDW+DEEC+KILKKCKE+I +S     K+I++E ++E  
Sbjct: 248 VSGDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSCRKIILVEIVME-- 307

Query: 310 KGEKKNKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQ 358
             EK+  + ++  L FD+ M+A    GKER+ +EW  L   AGFT + IT +  ++S+I+
Sbjct: 308 -DEKETHEATETKLFFDMQMLAIIT-GKERSEKEWGKLFFDAGFTNYKITRVLGLRSVIE 366

BLAST of Csa7G018730 vs. Swiss-Prot
Match: 6OMT_PAPSO ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum GN=6OMT PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 8.2e-71
Identity = 148/357 (41.46%), Postives = 214/357 (59.94%), Query Frame = 1

Query: 7   ESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNCS 66
           E+ S+ ++  QA  ++W+ I+ F E   +KC V L+IA+ + +   P++LS+L+S L  +
Sbjct: 2   ETVSKIDQQNQA--KIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVA 61

Query: 67  SSL----LHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSP 126
             +    L RI+R+L+H  +FK   ID     YS  P ++ L    E SM   +L     
Sbjct: 62  QPVNEDRLFRIMRYLVHMELFK---IDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDK 121

Query: 127 VMLAPWQHLSGHLK-NSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAII 186
             LAPW HL   L  N D   FE A GK +W Y   N E N LFN AMAC  +++ SA+ 
Sbjct: 122 DFLAPWHHLGDGLTGNCDA--FEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALA 181

Query: 187 EGCGDVF-DGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG 246
             C  +F DG+  LVDVGGG G+ +  + KA+P +K   +DLPHV+  SP+  N+  ++G
Sbjct: 182 NECKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISG 241

Query: 247 NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN 306
           +MF  +P+AD  F+K ILHDW+D+ECI+ILK+CKE++PK GGKVII++ +I+ +      
Sbjct: 242 DMFKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPK-GGKVIIVDVVIDMDSTHPYA 301

Query: 307 KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
           K    + L  DL MM +T  GKERT +EW  L   AGF +H +T I A+QS+I+ +P
Sbjct: 302 K----IRLTLDLDMMLNTG-GKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345

BLAST of Csa7G018730 vs. TrEMBL
Match: A0A0A0K629_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018730 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.2e-206
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 1

Query: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60
           MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120
           STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET
Sbjct: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120

Query: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI 180
           SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI
Sbjct: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI 180

Query: 181 IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG 240
           IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG
Sbjct: 181 IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG 240

Query: 241 NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN 300
           NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN
Sbjct: 241 NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN 300

Query: 301 KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
           KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP
Sbjct: 301 KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 357

BLAST of Csa7G018730 vs. TrEMBL
Match: A0A0A0K2N4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017720 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 5.3e-125
Identity = 213/352 (60.51%), Postives = 277/352 (78.69%), Query Frame = 1

Query: 6   KESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNC 65
           K    ++EE+ +A +++W+YIF F EMA +KC ++L+I D IES+GSP+TLSQLS+ LNC
Sbjct: 7   KSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLSTALNC 66

Query: 66  SSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVML 125
           S+SLL+RILRFL+ RGIFKQE  + N I Y QTP+SRLLA++  NSMAP LLLE+SPVML
Sbjct: 67  SASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLESSPVML 126

Query: 126 APWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMI-VSAIIEGC 185
           APW  LS  +K +   PFE AHGKD+W +A A+  HN++ N+AM+C A+++ V AI+E C
Sbjct: 127 APWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEEC 186

Query: 186 GDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFD 245
             +F+G+  LVDVGGGNG+ LS++VKA+PW+KGINFDLPHV+ +S QY  VEHV GNM D
Sbjct: 187 PQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVGGNMLD 246

Query: 246 FVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKNKKLS 305
            +P AD AF+ W+LHDWDDE CIKILK CK +I +  GKVII+EA+IE E+ E+ N KL 
Sbjct: 247 SIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKRGKVIIVEALIE-ERSEENNNKLG 306

Query: 306 DVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCF 357
           DVGLM D+VMMAHT  GKERT++EW  ++HQAGFT +TITPI+A+ S+IQ F
Sbjct: 307 DVGLMLDMVMMAHTKNGKERTSKEWGHVLHQAGFTQYTITPIRAVHSVIQAF 357

BLAST of Csa7G018730 vs. TrEMBL
Match: A0A061DXX0_THECC (RS-norcoclaurine 6-O-methyltransferase OS=Theobroma cacao GN=TCM_006540 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 2.0e-116
Identity = 198/344 (57.56%), Postives = 262/344 (76.16%), Query Frame = 1

Query: 14  EDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNCSSSLLHRI 73
           ED +A I +WRYIF F EMA +KC ++L IAD IE++GSP+TLS+LS+ L C  S LHRI
Sbjct: 14  EDAEAAIDVWRYIFGFVEMAVVKCAIELGIADAIENHGSPMTLSELSAALGCEPSRLHRI 73

Query: 74  LRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVMLAPWQHLSG 133
           +RF++H  +FK+E I +  IGY+QTP+SR L ++  NSMA L+LLE+SPVMLAPW  L  
Sbjct: 74  MRFMVHNQVFKEEPISQGSIGYAQTPLSRRLTSSGVNSMAALVLLESSPVMLAPWHSLRA 133

Query: 134 HLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAIIEGCGDVFDGVRC 193
            +  S   PFE AHGKD+W YAEAN  H+ L NEAMAC A++ V AIIEGC +VFDG+  
Sbjct: 134 RVLASGDSPFEAAHGKDIWSYAEANPGHSTLINEAMACAARVSVPAIIEGCPEVFDGLGS 193

Query: 194 LVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFDFVPNADVAF 253
           LVDVGGGNG+ L+ LVKA+PW++GINFDLPHVV A+ Q++ +E+V G+MF+ +P AD AF
Sbjct: 194 LVDVGGGNGTALTTLVKAFPWIRGINFDLPHVVAAATQFDGIENVGGDMFESIPKADAAF 253

Query: 254 LKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKNKKLSDVGLMFDLV 313
             W+LHDWDDEEC KILKKC+E+IP+  GKVII+EA++E    +  + KL  + LM D+V
Sbjct: 254 FMWVLHDWDDEECTKILKKCREAIPEDKGKVIIVEAVLE----DDTDDKLDFLRLMLDMV 313

Query: 314 MMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
           MMAHTN+GKERT +EW +++  AGFT   +  I+A+QS+I+ +P
Sbjct: 314 MMAHTNKGKERTLKEWRYVLGHAGFTRINVKRIRAVQSVIEAYP 353

BLAST of Csa7G018730 vs. TrEMBL
Match: A0A0D2R8U0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G038200 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 2.5e-114
Identity = 192/351 (54.70%), Postives = 265/351 (75.50%), Query Frame = 1

Query: 6   KESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNC 65
           K    +++ED +A +++W Y+F + ++A +KC ++L IAD I+ +GSP+TLSQL++TL C
Sbjct: 5   KVRGKKEDEDEKAEVEIWNYVFGYAKIAVVKCAIELGIADAIDKHGSPMTLSQLTTTLKC 64

Query: 66  SSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVML 125
               L+RILRFL+H  IFK+E + ++ IG++ TP+SR L  + E SMA  +LLE+SPVML
Sbjct: 65  EPPRLYRILRFLVHYQIFKEEPVTQDSIGFALTPLSRRLIRHGERSMAAFILLESSPVML 124

Query: 126 APWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAIIEGCG 185
           APW  LS  + +S   PFE AHGKD+W YAE N  H+ L +EAMAC A++ V A+IEGC 
Sbjct: 125 APWHSLSARVLDSGNSPFETAHGKDVWSYAEENPGHSKLIDEAMACDARVAVRALIEGCP 184

Query: 186 DVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFDF 245
            VFDG++ LVDVGGGNG+ LS+LVK +PWM GINFDLPHVV  +P+ + +E+V G+MF+ 
Sbjct: 185 QVFDGIKSLVDVGGGNGTALSMLVKEFPWMHGINFDLPHVVAVAPKVDGIEYVGGDMFEC 244

Query: 246 VPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKNKKLSD 305
           VP AD AF  W+LHDWDDEECI+ILKKC+E+IP+  GKVII+E+++E    E +N KL  
Sbjct: 245 VPRADAAFFMWVLHDWDDEECIQILKKCREAIPQDKGKVIIVESVLE----EDENDKLEF 304

Query: 306 VGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCF 357
           VGLM D+VMMAHTN+GKERT +EW +++ +AGFT   + PI A+QS+I+ +
Sbjct: 305 VGLMLDMVMMAHTNKGKERTLKEWKYVLGEAGFTRINVKPIHAVQSIIEAY 351

BLAST of Csa7G018730 vs. TrEMBL
Match: A0A0D2U960_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_N021400 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.2e-113
Identity = 193/350 (55.14%), Postives = 267/350 (76.29%), Query Frame = 1

Query: 7   ESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNCS 66
           E S  +EE+ +A + +W Y+F + ++A +KC ++L IAD+IE+YGSP+ LS+L++ L C 
Sbjct: 2   EVSGIEEEEARAEVDIWNYVFGYAKIAVVKCAIELGIADVIENYGSPMPLSELATALRCE 61

Query: 67  SSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVMLA 126
            S LHRI+RF++H  IFKQE I+++ +G+S TP+SR L  + E SMAP +LLE+SP  LA
Sbjct: 62  PSRLHRIMRFMVHDRIFKQEPINQHTVGFSSTPLSRRLIKDGEKSMAPFILLESSPTWLA 121

Query: 127 PWQHLSGHL--KNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAIIEGC 186
           PW  LS  +    ++  PFE+A+GKDLW YAEAN +   LFN A  C A++ V A IEGC
Sbjct: 122 PWHSLSARVLETGNNISPFEVANGKDLWSYAEANPDFRELFNNATGCDARLTVQATIEGC 181

Query: 187 GDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFD 246
            +VFDGV  LVDVGGGNG+ LS+LVKA+PW++GINFDLPHVV  +P+  ++E+V G+MF 
Sbjct: 182 PEVFDGVESLVDVGGGNGTALSLLVKAFPWIRGINFDLPHVVAVAPKSGSIENVGGDMFM 241

Query: 247 FVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKNKKLS 306
            +PNAD AFL WILHDWDDEECIKILKKC+E+IP+  GKVII+EA +E    +K++ +L 
Sbjct: 242 SIPNADAAFLMWILHDWDDEECIKILKKCREAIPEDKGKVIIVEAALEE---DKEDDELG 301

Query: 307 DVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQ 355
            VGLM D+ MMAHT++GKERT +EW++++ Q+GFT  T+ PI+A+QS+I+
Sbjct: 302 FVGLMLDMAMMAHTDKGKERTLKEWSYVLRQSGFTRFTVNPIRAVQSVIE 348

BLAST of Csa7G018730 vs. TAIR10
Match: AT4G35160.1 (AT4G35160.1 O-methyltransferase family protein)

HSP 1 Score: 338.2 bits (866), Expect = 6.2e-93
Identity = 167/354 (47.18%), Postives = 239/354 (67.51%), Query Frame = 1

Query: 13  EEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGS--PVTLSQLSSTLNCSSSLL 72
           +E+ +A + +W+Y+F F ++AA KC +DLKI + IE++ S  PVTL++LSS ++ S S L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 73  HRILRFLIHRGIFKQ-ETIDENQIGYSQTPMSRLLATNVEN--SMAPLLLLETSPVMLAP 132
            RI+RFL+H+GIFK+  T D    GY  TP+SR L     +  S+AP +L ET+P MLAP
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 133 WQHLSGHLKN----SDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAIIEG 192
           W  LS  + +    S   PF+  HGKD+W +A+ N   + + NEAMAC A+ +V  +   
Sbjct: 144 WLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVPRVAGA 203

Query: 193 CGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMF 252
           C  +FDGV  +VDVGGG G T+ +LVK +PW+KG NFDLPHV+  +   + VE+V G+MF
Sbjct: 204 CHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGDMF 263

Query: 253 DFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKG----EKK 312
           D +P  D  F+KW+LHDW D++CIKILK CKE++P + GKV+I+E++I   K     +++
Sbjct: 264 DSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVDER 323

Query: 313 NKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLI 354
           ++KL  V LM D+VMMAHT+ GKERT +EW F++ +AGF  + +  I  +QSLI
Sbjct: 324 DEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

BLAST of Csa7G018730 vs. TAIR10
Match: AT4G35150.1 (AT4G35150.1 O-methyltransferase family protein)

HSP 1 Score: 288.1 bits (736), Expect = 7.4e-78
Identity = 147/347 (42.36%), Postives = 209/347 (60.23%), Query Frame = 1

Query: 13  EEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGS--PVTLSQLSSTLNCSSSLL 72
           +E+ +A + +WRY+F F ++AA KC +DLKI + IE++ S  PVTLS+LSS ++ S S L
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHL 69

Query: 73  HRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVMLAPWQH 132
            RI+RFL+H+G+F             + P    LAT   N+     ++ T          
Sbjct: 70  RRIMRFLVHQGLF------------KEVPTKDGLATGYTNTPLSRRMMITK--------- 129

Query: 133 LSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAIIEGCGDVFDG 192
                           HGKDLW +A+ N  H+ L NEAMAC A+ +V  +   C  +FDG
Sbjct: 130 ---------------LHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQGLFDG 189

Query: 193 VRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFDFVPNAD 252
           V  +VDVGGG G T+ ILVK +PW+KG NFDLPHV+  +   + VE+V G+MFD +P +D
Sbjct: 190 VATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPASD 249

Query: 253 VAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEK----GEKKNKKLSDV 312
              +KW+LHDW D++CIKILK CKE++  + GKV+I+E +I  +K     E+++ KL  V
Sbjct: 250 AVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLEHV 309

Query: 313 GLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLI 354
            L  D+VMM HT+ GKERT +EW F++ +AGF  + +     +QSLI
Sbjct: 310 RLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320

BLAST of Csa7G018730 vs. TAIR10
Match: AT5G54160.1 (AT5G54160.1 O-methyltransferase 1)

HSP 1 Score: 189.1 bits (479), Expect = 4.7e-48
Identity = 113/315 (35.87%), Postives = 173/315 (54.92%), Query Frame = 1

Query: 34  AIKCVVDLKIADIIESYGSPVTLSQLSSTLNCSSS----LLHRILRFLIHRGIF----KQ 93
           A+K  ++L + +I+   GSP++ ++++S L   +     +L RILR L    +     ++
Sbjct: 35  ALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRK 94

Query: 94  ETIDENQIGYSQTPMSRLLATNVEN-SMAPLLLLETSPVMLAPWQHLSGHLKNSDTLPFE 153
            + D  +  Y   P+ + L  N +  S+A L L+    V++  W HL   + +   +PF 
Sbjct: 95  LSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG-IPFN 154

Query: 154 IAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAIIEGCGDVFDGVRCLVDVGGGNGST 213
            A+G   + Y   +   N +FN  M+  + + +  I+E     F+G+  LVDVGGG G+T
Sbjct: 155 KAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIGAT 214

Query: 214 LSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFDFVPNADVAFLKWILHDWDDE 273
           L ++V  YP +KGINFDLPHV+  +P +  +EHV G+MF  VP  D  F+KWI HDW DE
Sbjct: 215 LKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDE 274

Query: 274 ECIKILKKCKESIPKSGGKVIIIEAII-EAEKGEKKNKKLSDVGLMFDLVMMAHTNRGKE 333
            C+K LK C ES+P+  GKVI+ E I+ E        K++  V    D +M+AH   GKE
Sbjct: 275 HCVKFLKNCYESLPED-GKVILAECILPETPDSSLSTKQVVHV----DCIMLAHNPGGKE 334

Query: 334 RTAQEWAFLIHQAGF 339
           RT +E+  L   +GF
Sbjct: 335 RTEKEFEALAKASGF 342

BLAST of Csa7G018730 vs. TAIR10
Match: AT1G51990.1 (AT1G51990.1 O-methyltransferase family protein)

HSP 1 Score: 179.9 bits (455), Expect = 2.8e-45
Identity = 103/296 (34.80%), Postives = 168/296 (56.76%), Query Frame = 1

Query: 49  SYGSPVTLSQLSSTLNCSSSLL-HRILRFLIHRGIFKQETIDENQ----IGYSQTPMSRL 108
           SY SPV L+ +++  N  + ++  R+LRFL+   +   + + + +      Y    + + 
Sbjct: 52  SYLSPVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKK 111

Query: 109 LATNVEN-SMAPLLLLETSPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHN 168
           L  + +  S+AP +L   +      W +L+  ++      +E A+   ++ Y + N    
Sbjct: 112 LIKDEDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKNENLK 171

Query: 169 LLFNEAMACGAKMIVSAIIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDL 228
            +FNE+M     +++  I+E     F+GV   VDVGG  GS L+ ++  YP +KGINFDL
Sbjct: 172 KIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDL 231

Query: 229 PHVVCASPQYENVEHVAGNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGG 288
           PH+V  +PQ   VEH+ G+MFD +P  +V  +KWILHDW+DE+C++ILK CK+++P++ G
Sbjct: 232 PHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPET-G 291

Query: 289 KVIIIEAIIEAEKGEKKNKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGF 339
           ++I+IE I+  E  E      +   L  DL MM+ T+ GKERT +E+  L  +AGF
Sbjct: 292 RIIVIEMIVPREVSE--TDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGF 343

BLAST of Csa7G018730 vs. TAIR10
Match: AT5G53810.1 (AT5G53810.1 O-methyltransferase family protein)

HSP 1 Score: 178.7 bits (452), Expect = 6.3e-45
Identity = 119/349 (34.10%), Postives = 180/349 (51.58%), Query Frame = 1

Query: 11  EKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYG------SPVTLSQLSSTLN 70
           E+E + +A +   R          +K  ++L + D I + G      SP  ++    T  
Sbjct: 18  EQEVEEEARLLARRLANAAASPMVLKAALELGVIDTITTVGGGDLWLSPSEIALRLPTKP 77

Query: 71  CS---SSLLHRILRFLIHRGIFKQET-IDEN-QIG-----YSQTPMSRLL---ATNVENS 130
           C+    +LL R+LRFL+   + K  T I+EN Q G     Y+  P+ + L   + +V  S
Sbjct: 78  CNLEAPALLDRMLRFLVSHSVLKCRTVIEENGQTGKVERVYAAEPVCKYLLNKSDDVSGS 137

Query: 131 MAPLLLLETSPVMLAPWQHLSGH-LKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMA 190
            A L +L+ S V +  W HL    L+  D   F  AHG  L+ Y +A+     +FN AM 
Sbjct: 138 FASLFMLDLSDVFIKTWTHLEDVILEGRDA--FSSAHGMKLFEYIQADERFGKVFNRAML 197

Query: 191 CGAKMIVSAIIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASP 250
             + M+   +++   + F  V+ LVDVGGG G+TL ++   YP + GINFDL  V+  + 
Sbjct: 198 ESSTMVTEKVLKFY-EGFKDVKTLVDVGGGLGNTLGLITSKYPHLIGINFDLAPVLANAH 257

Query: 251 QYENVEHVAGNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAI 310
            Y  V HVAG+MF  +P  D  F+KWILHDW DE+C+ ILK C +S+ +  GK+II+E +
Sbjct: 258 SYPGVNHVAGDMFIKIPKGDAIFMKWILHDWTDEQCVAILKNCWKSL-EENGKLIIVEMV 317

Query: 311 IEAEKGEKKNKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFT 340
              E   K     S++    D+ M+   + GKER   E+  L + +GF+
Sbjct: 318 TPVE--AKSGDICSNIVFGMDMTMLTQCSGGKERDLYEFENLAYASGFS 360

BLAST of Csa7G018730 vs. NCBI nr
Match: gi|449470501|ref|XP_004152955.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 726.9 bits (1875), Expect = 1.8e-206
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 1

Query: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60
           MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120
           STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET
Sbjct: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120

Query: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI 180
           SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI
Sbjct: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI 180

Query: 181 IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG 240
           IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG
Sbjct: 181 IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG 240

Query: 241 NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN 300
           NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN
Sbjct: 241 NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN 300

Query: 301 KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
           KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP
Sbjct: 301 KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 357

BLAST of Csa7G018730 vs. NCBI nr
Match: gi|659094136|ref|XP_008447904.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 679.1 bits (1751), Expect = 4.2e-192
Identity = 333/358 (93.02%), Postives = 345/358 (96.37%), Query Frame = 1

Query: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60
           MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKC +DLKIADIIESYGSPVTLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120
           STLNCSSSLL+RILRFLIHRGIFK+ETIDENQIGYSQTPMSRLLA NVENSMAPLLLLET
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETIDENQIGYSQTPMSRLLAINVENSMAPLLLLET 120

Query: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI 180
           SPVMLAPWQHLS HLKNSDT PFEIAHGKDLW YAEANHEHNLLFNEAMAC AK+IVSAI
Sbjct: 121 SPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVSAI 180

Query: 181 IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG 240
           IEGCGDVFDGV CLVDVGGGNGSTL+ILVK  PWMKGINFDLPHVVCASPQYENVEHVAG
Sbjct: 181 IEGCGDVFDGVGCLVDVGGGNGSTLNILVKVCPWMKGINFDLPHVVCASPQYENVEHVAG 240

Query: 241 NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEA-IIEAEKGEKK 300
           NMFDFVP+ADVAFLKWILHDW+DEECIKILKKCKE+IPKSGGKVIIIEA IIEAEKGEK 
Sbjct: 241 NMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAEKGEKM 300

Query: 301 NKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
            KKLSDVGLMFDLVMMAHTN+G+ERTA+EWAFLIHQAGFT HTITP+QAIQSLIQCFP
Sbjct: 301 KKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQCFP 358

BLAST of Csa7G018730 vs. NCBI nr
Match: gi|449454368|ref|XP_004144927.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 455.7 bits (1171), Expect = 7.6e-125
Identity = 213/352 (60.51%), Postives = 277/352 (78.69%), Query Frame = 1

Query: 6   KESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNC 65
           K    ++EE+ +A +++W+YIF F EMA +KC ++L+I D IES+GSP+TLSQLS+ LNC
Sbjct: 7   KSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLSTALNC 66

Query: 66  SSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVML 125
           S+SLL+RILRFL+ RGIFKQE  + N I Y QTP+SRLLA++  NSMAP LLLE+SPVML
Sbjct: 67  SASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLESSPVML 126

Query: 126 APWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMI-VSAIIEGC 185
           APW  LS  +K +   PFE AHGKD+W +A A+  HN++ N+AM+C A+++ V AI+E C
Sbjct: 127 APWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEEC 186

Query: 186 GDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFD 245
             +F+G+  LVDVGGGNG+ LS++VKA+PW+KGINFDLPHV+ +S QY  VEHV GNM D
Sbjct: 187 PQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVGGNMLD 246

Query: 246 FVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKNKKLS 305
            +P AD AF+ W+LHDWDDE CIKILK CK +I +  GKVII+EA+IE E+ E+ N KL 
Sbjct: 247 SIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKRGKVIIVEALIE-ERSEENNNKLG 306

Query: 306 DVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCF 357
           DVGLM D+VMMAHT  GKERT++EW  ++HQAGFT +TITPI+A+ S+IQ F
Sbjct: 307 DVGLMLDMVMMAHTKNGKERTSKEWGHVLHQAGFTQYTITPIRAVHSVIQAF 357

BLAST of Csa7G018730 vs. NCBI nr
Match: gi|659094140|ref|XP_008447906.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 451.4 bits (1160), Expect = 1.4e-123
Identity = 215/350 (61.43%), Postives = 272/350 (77.71%), Query Frame = 1

Query: 9   SSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNCSSS 68
           S  K+E+ +A +Q+W+YIF F EMA +KC ++L+I D IES+GSP+TLSQLS+ LNCSSS
Sbjct: 8   SISKKEEEEARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLSTALNCSSS 67

Query: 69  LLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVMLAPW 128
           LL+RILRFL+ RGIFKQE  +EN I Y  TP+SRLLA++  NSMAPLLLLE+SPVMLAPW
Sbjct: 68  LLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLESSPVMLAPW 127

Query: 129 QHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKM-IVSAIIEGCGDV 188
             LS  +K +   PFE AHGKD+W +A A+  HN++ N+AM+C A++  V AI+E C  +
Sbjct: 128 HRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVPAILEDCPQI 187

Query: 189 FDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFDFVP 248
           F+G+  LVDVGGGNG+ LS++VKA+PW+KGINFDLPHV+ +S QY  VEHV GNM D +P
Sbjct: 188 FEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVGGNMLDSIP 247

Query: 249 NADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAII-EAEKGEKKNKKLSDV 308
            AD AF+ W+LHDWDDE CIKILK CKE+I +  GKVII+EAII E  + E  N  L DV
Sbjct: 248 KADAAFIMWVLHDWDDETCIKILKNCKEAISEKRGKVIIVEAIIEERSEEENNNNNLGDV 307

Query: 309 GLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCF 357
           GLM D+VMMAHT  GKERT +EW  ++ QAGFT +TITPI+A+ S+IQ F
Sbjct: 308 GLMLDMVMMAHTENGKERTIKEWGNVLRQAGFTRYTITPIRAVHSVIQGF 357

BLAST of Csa7G018730 vs. NCBI nr
Match: gi|449454255|ref|XP_004144871.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 438.3 bits (1126), Expect = 1.3e-119
Identity = 215/351 (61.25%), Postives = 269/351 (76.64%), Query Frame = 1

Query: 12  KEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLSSTLNCSSSLLH 71
           +EED    +Q+W+YIF F EMA IKC ++LKI D+IES+G  +TLSQLSS+LNCS  LLH
Sbjct: 15  EEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLLLH 74

Query: 72  RILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVMLAPWQHL 131
           RILRFL+HRGIFK+E   +    YS TPMSRLL T   NSMAPL LLE+SPVM+APW +L
Sbjct: 75  RILRFLVHRGIFKEEKTTKC---YSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPWHNL 134

Query: 132 SGHLKNSD----TLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIV-SAIIEGCGD 191
           S  +K SD    + PFE+AHG DLW YA AN  H+ L NE+MAC A++++  A++E CG+
Sbjct: 135 SACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLVILPAVLERCGE 194

Query: 192 VFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAGNMFDFV 251
           +FDGV CLVDVGGGNG+ LSILVKA PW+KGINFDLPHVV  S +YE ++HV GNMFD V
Sbjct: 195 IFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGIQHVGGNMFDSV 254

Query: 252 PNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKNKKLSDV 311
           P AD AF+  +LHDWDDEECIKILK CKE+IP+  GKVII+E +I+    +++  K SD 
Sbjct: 255 PKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDE---KEEISKYSDA 314

Query: 312 GLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 358
            LM D++MMAHT +GKERT +EWA ++ +AGF+ +TITP   IQS+IQ FP
Sbjct: 315 RLMMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAFP 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
4OMT2_PAPSO2.2e-7641.573'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu... [more]
6OMT_COPJA5.5e-7540.83(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1[more]
7OMT_PAPSO2.3e-7340.67(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum GN=7OMT PE=1 SV=1[more]
Q8OMT_MENPI7.4e-7239.948-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita GN=OMT2 PE=1 SV=1[more]
6OMT_PAPSO8.2e-7141.46(RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum GN=6OMT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K629_CUCSA1.2e-206100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018730 PE=3 SV=1[more]
A0A0A0K2N4_CUCSA5.3e-12560.51Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017720 PE=3 SV=1[more]
A0A061DXX0_THECC2.0e-11657.56RS-norcoclaurine 6-O-methyltransferase OS=Theobroma cacao GN=TCM_006540 PE=3 SV=... [more]
A0A0D2R8U0_GOSRA2.5e-11454.70Uncharacterized protein OS=Gossypium raimondii GN=B456_008G038200 PE=3 SV=1[more]
A0A0D2U960_GOSRA1.2e-11355.14Uncharacterized protein OS=Gossypium raimondii GN=B456_N021400 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35160.16.2e-9347.18 O-methyltransferase family protein[more]
AT4G35150.17.4e-7842.36 O-methyltransferase family protein[more]
AT5G54160.14.7e-4835.87 O-methyltransferase 1[more]
AT1G51990.12.8e-4534.80 O-methyltransferase family protein[more]
AT5G53810.16.3e-4534.10 O-methyltransferase family protein[more]
Match NameE-valueIdentityDescription
gi|449470501|ref|XP_004152955.1|1.8e-206100.00PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus][more]
gi|659094136|ref|XP_008447904.1|4.2e-19293.02PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo][more]
gi|449454368|ref|XP_004144927.1|7.6e-12560.51PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis sativus][more]
gi|659094140|ref|XP_008447906.1|1.4e-12361.43PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo][more]
gi|449454255|ref|XP_004144871.1|1.3e-11961.25PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001077O_MeTrfase_2
IPR011991Winged helix-turn-helix DNA-binding domain
IPR012967Plant_MeTrfase_dimerisation
IPR016461O-MeTrfase_COMT
Vocabulary: Molecular Function
TermDefinition
GO:0008171O-methyltransferase activity
GO:0046983protein dimerization activity
GO:0008168methyltransferase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008168 methyltransferase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU093494cucumber EST collection version 3.0transcribed_cluster
CU110298cucumber EST collection version 3.0transcribed_cluster
CU116285cucumber EST collection version 3.0transcribed_cluster
CU132598cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa7G018730.1Csa7G018730.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU116285CU116285transcribed_cluster
CU093494CU093494transcribed_cluster
CU110298CU110298transcribed_cluster
CU132598CU132598transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001077O-methyltransferase, family 2PFAMPF00891Methyltransf_2coord: 119..340
score: 6.6
IPR011991Winged helix-turn-helix DNA-binding domainGENE3DG3DSA:1.10.10.10coord: 7..104
score: 1.9
IPR011991Winged helix-turn-helix DNA-binding domainunknownSSF46785"Winged helix" DNA-binding domaincoord: 13..107
score: 7.31
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 34..77
score: 2.
IPR016461O-methyltransferase COMT-typePIRPIRSF005739O-mtasecoord: 1..357
score: 1.5E
IPR016461O-methyltransferase COMT-typePROFILEPS51683SAM_OMT_IIcoord: 23..357
score: 77
NoneNo IPR availablePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 1..357
score: 2.1E
NoneNo IPR availablePANTHERPTHR11746:SF98O-METHYLTRANSFERASE FAMILY PROTEINcoord: 1..357
score: 2.1E