Csa6G307360 (gene) Cucumber (Chinese Long) v2

NameCsa6G307360
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPectinesterase inhibitor; contains IPR006501 (Pectinesterase inhibitor)
LocationChr6 : 14674324 .. 14674872 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAGTTCCAATTTGAATTTTGGAGTTTTTCTATTTGGTTTTCTTTTGTCCAATAATGTCATTCCCTTGAATGCTGATACGATACTATCGAACATCGACACTTTAGCATCAAATATATGTCCACAAACCAGTAATCCATCGTTTTGTGCAAGCATATTGGAAAATGCAAACAACATAGACTTGAAAGCTTTGATCGCTTACAGCCTAAAACTTGCTCACACAAATGCAGGAAAATCTATGACTCTAGCCAAAGCACTTGCAGTACTGACAACCAATACTCTGCTTAAGAAACAATATTTGTTTTGCTTTGAGAACTACGACGAGGCGATGTGTGACATTGAAAAAGCTAAAAATGATTTGGTATTTGGTGACTATAGTGGTGTTAATCTTGCGATTTCTGATGCAATGATGATAGCTGATGACTGTCATGATAGCTTCAAACAACCGTTGAAAGATATGTCATTGCTTCCAAATAATACCAAGATGTTGAAAGACATTTATAGCATTATTTTAGTTATATCAAGTATTCTACCAAAGAATATATAA

mRNA sequence

ATGATCAGTTCCAATTTGAATTTTGGAGTTTTTCTATTTGGTTTTCTTTTGTCCAATAATGTCATTCCCTTGAATGCTGATACGATACTATCGAACATCGACACTTTAGCATCAAATATATGTCCACAAACCAGTAATCCATCGTTTTGTGCAAGCATATTGGAAAATGCAAACAACATAGACTTGAAAGCTTTGATCGCTTACAGCCTAAAACTTGCTCACACAAATGCAGGAAAATCTATGACTCTAGCCAAAGCACTTGCAGTACTGACAACCAATACTCTGCTTAAGAAACAATATTTGTTTTGCTTTGAGAACTACGACGAGGCGATGTGTGACATTGAAAAAGCTAAAAATGATTTGGTATTTGGTGACTATAGTGGTGTTAATCTTGCGATTTCTGATGCAATGATGATAGCTGATGACTGTCATGATAGCTTCAAACAACCGTTGAAAGATATGTCATTGCTTCCAAATAATACCAAGATGTTGAAAGACATTTATAGCATTATTTTAGTTATATCAAGTATTCTACCAAAGAATATATAA

Coding sequence (CDS)

ATGATCAGTTCCAATTTGAATTTTGGAGTTTTTCTATTTGGTTTTCTTTTGTCCAATAATGTCATTCCCTTGAATGCTGATACGATACTATCGAACATCGACACTTTAGCATCAAATATATGTCCACAAACCAGTAATCCATCGTTTTGTGCAAGCATATTGGAAAATGCAAACAACATAGACTTGAAAGCTTTGATCGCTTACAGCCTAAAACTTGCTCACACAAATGCAGGAAAATCTATGACTCTAGCCAAAGCACTTGCAGTACTGACAACCAATACTCTGCTTAAGAAACAATATTTGTTTTGCTTTGAGAACTACGACGAGGCGATGTGTGACATTGAAAAAGCTAAAAATGATTTGGTATTTGGTGACTATAGTGGTGTTAATCTTGCGATTTCTGATGCAATGATGATAGCTGATGACTGTCATGATAGCTTCAAACAACCGTTGAAAGATATGTCATTGCTTCCAAATAATACCAAGATGTTGAAAGACATTTATAGCATTATTTTAGTTATATCAAGTATTCTACCAAAGAATATATAA

Protein sequence

MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSILPKNI*
BLAST of Csa6G307360 vs. Swiss-Prot
Match: PMEI_ACTDE (Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 1.2e-17
Identity = 60/182 (32.97%), Postives = 94/182 (51.65%), Query Frame = 1

Query: 1   MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILEN---A 60
           M  S  +  +F+   L+   + PL+    +   + L S ICP+T NPS C   LE+   +
Sbjct: 1   MAFSYCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRS 60

Query: 61  NNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKA 120
            + DLK L  +S+ +A  +A ++  +  +L    T+  LK +Y  C ENY +A+  + +A
Sbjct: 61  ASKDLKGLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQA 120

Query: 121 KNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSI 180
           K  L  GDY+ +N+  S A   A  C DSF+ P    + L      L+D+  I+LVIS++
Sbjct: 121 KQFLTSGDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNL 180

BLAST of Csa6G307360 vs. Swiss-Prot
Match: PMEI1_ARATH (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.1e-13
Identity = 51/147 (34.69%), Postives = 78/147 (53.06%), Query Frame = 1

Query: 38  SNICPQTSNPSFCASILENA-NNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLT--TNT 97
           S IC +T NPSFC   L     + +L+AL   +L    T A  + TL K  +++    + 
Sbjct: 32  STICDKTLNPSFCLKFLNTKFASPNLQALAKTTLD--STQARATQTLKKLQSIIDGGVDP 91

Query: 98  LLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDM 157
             K  Y  C + Y+ A+ ++E+A   L  GD  G+N+ +S A+  AD C D  K+     
Sbjct: 92  RSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSVD 151

Query: 158 SLLPNNTKMLKDIYSIILVISSILPKN 182
           S + NN+K +K++  I LVIS++LP+N
Sbjct: 152 SSVVNNSKTIKNLCGIALVISNMLPRN 176

BLAST of Csa6G307360 vs. Swiss-Prot
Match: PMEI2_ARATH (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.4e-11
Identity = 43/145 (29.66%), Postives = 72/145 (49.66%), Query Frame = 1

Query: 40  ICPQTSNPSFCASILEN---ANNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLL 99
           IC +  N SFC S +++    +  DL+ L   +   A T+A +     ++L    TN  +
Sbjct: 33  ICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTATNPTM 92

Query: 100 KKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSL 159
           KK Y  C ++Y  A+  +  AK  L  GD  G+N+ +S AM     C    +Q + D  +
Sbjct: 93  KKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTC----EQDMADFKV 152

Query: 160 LPN---NTKMLKDIYSIILVISSIL 179
            P+   N+   ++I  I+LVIS+++
Sbjct: 153 DPSAVKNSGDFQNICGIVLVISNMM 173

BLAST of Csa6G307360 vs. TrEMBL
Match: A0A0A0KCC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307360 PE=4 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 2.0e-96
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 1

Query: 1   MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNI 60
           MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNI
Sbjct: 1   MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNI 60

Query: 61  DLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKND 120
           DLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKND
Sbjct: 61  DLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKND 120

Query: 121 LVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSILPK 180
           LVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSILPK
Sbjct: 121 LVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSILPK 180

Query: 181 NI 183
           NI
Sbjct: 181 NI 182

BLAST of Csa6G307360 vs. TrEMBL
Match: A0A0A0KEW6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307350 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.6e-37
Identity = 97/186 (52.15%), Postives = 124/186 (66.67%), Query Frame = 1

Query: 1   MISSNLN---FG----VFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASI 60
           MI+ NLN   FG    VF+F FL  + V+P  A T  SN   +AS+ICP+T NP FC  +
Sbjct: 1   MINLNLNVRIFGSIAIVFVFSFLFFS-VVPSRATTTPSN--DVASSICPKTRNPPFCVDV 60

Query: 61  LENANNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCD 120
           L++A + DLK L  Y+L LA+ NA KS  LAK+LA +TTN  LK QYL C+E+Y+EA  D
Sbjct: 61  LKSAGSTDLKVLATYTLNLANENALKSTNLAKSLAAMTTNPPLKNQYLSCYESYEEATSD 120

Query: 121 IEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILV 180
           IE AK++L  GD++GVN+A S  M    DC DSFKQ   D SLL +  K L D+ SIILV
Sbjct: 121 IENAKSNLASGDFNGVNIATSGVMTSVSDCLDSFKQLRIDPSLLKDG-KTLNDVCSIILV 180

BLAST of Csa6G307360 vs. TrEMBL
Match: A0A0A0KCV8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307340 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 8.9e-36
Identity = 79/148 (53.38%), Postives = 104/148 (70.27%), Query Frame = 1

Query: 30  LSNIDTLASNICPQTSNPSFCASILENANNIDLKALIAYSLKLAHTNAGKSMTLAKALAV 89
           +++ + + S ICP+TSNP FC+S+L++A   DLK L+ Y+L LAHTNAGKS+TLA +LA 
Sbjct: 24  VASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLVVYTLNLAHTNAGKSLTLANSLAK 83

Query: 90  LTTNTLLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQ 149
             TN  LK++Y  C E+YDEA+ DIE A+ DL   D++GVN+  S AM    DC D F Q
Sbjct: 84  TATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNGVNIVTSGAMTNIGDCQDKFAQ 143

Query: 150 PLKDMSLLPNNTKMLKDIYSIILVISSI 178
           P KD SLL  N K + DI SIILVIS++
Sbjct: 144 PPKDTSLLLENGKTINDICSIILVISNL 171

BLAST of Csa6G307360 vs. TrEMBL
Match: A0A0A0KHY0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307370 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.0e-16
Identity = 65/187 (34.76%), Postives = 99/187 (52.94%), Query Frame = 1

Query: 3   SSNLNFGV------FLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILEN 62
           S N N GV      FLFG L   +V+ L+ADT   N+  L + ICP+T N +FC ++L+ 
Sbjct: 9   SFNSNLGVVSSILIFLFG-LFFFSVVLLHADTGSPNV--LTTTICPKTRNSTFCKTVLKP 68

Query: 63  ANNID--LKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDI 122
               D  L  +  Y+L  AHT   + +  A+ LA   T+ LLK++Y  C   +D     +
Sbjct: 69  VGKSDAALLKVANYTLTFAHTTTVEGLHHAQRLATEATDPLLKQRYSECSRRFDFVTKGL 128

Query: 123 EKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVI 182
           E+A   L  G Y  ++ A   A++ AD C + FK+P  + S LP   K + DI  I + +
Sbjct: 129 EEAIEALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKNIGDICDIAVSV 188

BLAST of Csa6G307360 vs. TrEMBL
Match: A0A072TNA9_MEDTR (Plant invertase/pectin methylesterase inhibitor OS=Medicago truncatula GN=MTR_8g032810 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.3e-14
Identity = 50/146 (34.25%), Postives = 83/146 (56.85%), Query Frame = 1

Query: 40  ICPQTSNPSFCASILEN---ANNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLL 99
           IC + +NPSFC++ILE+       DL +L +Y++ +  TN   +++L   LA  + +  L
Sbjct: 29  ICKKATNPSFCSTILESKPGGAGKDLVSLASYTIDVLRTNVSNTISLITKLAAQSGSDSL 88

Query: 100 KK-QYLFCFENY---DEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLK 159
           K+ +Y  C   +   D A+ ++E+A+  L   DY+GVN+ I+  M   D+C      P  
Sbjct: 89  KQNRYKNCLSRFGMDDGALGEVEEAQKVLKDSDYNGVNMHITSVMTAVDECLSKDSSPDN 148

Query: 160 DMSLLPNNTKMLKDIYSIILVISSIL 179
           D SLLP + + +  I  IIL+IS++L
Sbjct: 149 DTSLLPKDVECVNQIAQIILIISNVL 174

BLAST of Csa6G307360 vs. TAIR10
Match: AT1G48020.1 (AT1G48020.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 76.6 bits (187), Expect = 1.7e-14
Identity = 51/147 (34.69%), Postives = 78/147 (53.06%), Query Frame = 1

Query: 38  SNICPQTSNPSFCASILENA-NNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLT--TNT 97
           S IC +T NPSFC   L     + +L+AL   +L    T A  + TL K  +++    + 
Sbjct: 32  STICDKTLNPSFCLKFLNTKFASPNLQALAKTTLD--STQARATQTLKKLQSIIDGGVDP 91

Query: 98  LLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDM 157
             K  Y  C + Y+ A+ ++E+A   L  GD  G+N+ +S A+  AD C D  K+     
Sbjct: 92  RSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSVD 151

Query: 158 SLLPNNTKMLKDIYSIILVISSILPKN 182
           S + NN+K +K++  I LVIS++LP+N
Sbjct: 152 SSVVNNSKTIKNLCGIALVISNMLPRN 176

BLAST of Csa6G307360 vs. TAIR10
Match: AT3G17220.1 (AT3G17220.1 pectin methylesterase inhibitor 2)

HSP 1 Score: 68.9 bits (167), Expect = 3.6e-12
Identity = 43/145 (29.66%), Postives = 72/145 (49.66%), Query Frame = 1

Query: 40  ICPQTSNPSFCASILEN---ANNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLL 99
           IC +  N SFC S +++    +  DL+ L   +   A T+A +     ++L    TN  +
Sbjct: 33  ICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTATNPTM 92

Query: 100 KKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSL 159
           KK Y  C ++Y  A+  +  AK  L  GD  G+N+ +S AM     C    +Q + D  +
Sbjct: 93  KKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTC----EQDMADFKV 152

Query: 160 LPN---NTKMLKDIYSIILVISSIL 179
            P+   N+   ++I  I+LVIS+++
Sbjct: 153 DPSAVKNSGDFQNICGIVLVISNMM 173

BLAST of Csa6G307360 vs. TAIR10
Match: AT3G05741.1 (AT3G05741.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 47.8 bits (112), Expect = 8.6e-06
Identity = 43/181 (23.76%), Postives = 79/181 (43.65%), Query Frame = 1

Query: 9   GVFLFGFLLSNNVI---PLNADTILSNIDT--LASNICPQTS--NPSFCASIL---ENAN 68
           G ++  FLL   ++   P ++     +I T  L +N C      +  FCA      +   
Sbjct: 10  GYYILHFLLLLIILIITPSSSSLSFKDIVTKELINNFCSNLEMFDRQFCAKWFNADQKTK 69

Query: 69  NIDLKALIAYSLKLAHTNAGKSMTLAKALAVLT-TNTLLKKQYLFCFENYDEAMCDIEKA 128
           +I ++  I+  +K       ++  L   LA  +  +  LK  Y  C  +Y +A+ ++EKA
Sbjct: 70  SISVQGFISLRVKETREFGLQTQALMSKLAKSSGKDQQLKGSYESCVASYGQAIKELEKA 129

Query: 129 KNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSI 179
           +  L    ++   +AISDA   A DC D+F+ P  +  L+ N  +   ++  +     S+
Sbjct: 130 QKFLSSNSFTQALVAISDAFYKAGDCKDAFEGPSNEPPLVFNRVENFANMCYVTWSFGSL 189

BLAST of Csa6G307360 vs. NCBI nr
Match: gi|700192171|gb|KGN47375.1| (hypothetical protein Csa_6G307360 [Cucumis sativus])

HSP 1 Score: 359.8 bits (922), Expect = 2.9e-96
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 1

Query: 1   MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNI 60
           MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNI
Sbjct: 1   MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNI 60

Query: 61  DLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKND 120
           DLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKND
Sbjct: 61  DLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKND 120

Query: 121 LVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSILPK 180
           LVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSILPK
Sbjct: 121 LVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSILPK 180

Query: 181 NI 183
           NI
Sbjct: 181 NI 182

BLAST of Csa6G307360 vs. NCBI nr
Match: gi|659117597|ref|XP_008458685.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 292.0 bits (746), Expect = 7.4e-76
Identity = 150/174 (86.21%), Postives = 158/174 (90.80%), Query Frame = 1

Query: 10  VFLFGFLLSNN-VIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNIDLKALIAY 69
           VFLFG LLSN   IPLNA+TI SN D L SNICPQTSNPSFCASILE+ANNIDLKALIAY
Sbjct: 2   VFLFGLLLSNTYAIPLNANTIPSNGDILVSNICPQTSNPSFCASILESANNIDLKALIAY 61

Query: 70  SLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSG 129
            L LAHTNAGKSMTLAKALAVLTTN  LKKQYL CFENYDEAMCDIEKAK+DL FGDY+G
Sbjct: 62  GLNLAHTNAGKSMTLAKALAVLTTNPRLKKQYLSCFENYDEAMCDIEKAKSDLAFGDYNG 121

Query: 130 VNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSILPKNI 183
           VNLAISDAMMIADDCHD+F+Q LKDMSLLPNNT+ LKDIYSIILVISSILP+NI
Sbjct: 122 VNLAISDAMMIADDCHDNFEQSLKDMSLLPNNTRTLKDIYSIILVISSILPRNI 175

BLAST of Csa6G307360 vs. NCBI nr
Match: gi|659117595|ref|XP_008458684.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 172.6 bits (436), Expect = 6.6e-40
Identity = 91/169 (53.85%), Postives = 119/169 (70.41%), Query Frame = 1

Query: 10  VFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNIDLKALIAYS 69
           VF+  FL SN V+   A+TI  + D +AS+ICP+T NPSFC  +L++A + DLK L  Y+
Sbjct: 18  VFVCSFLFSN-VVSSRANTITPSND-VASSICPKTRNPSFCVDVLKSAGSTDLKVLATYT 77

Query: 70  LKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGV 129
           L L +TNA KS+ L K+LA  TTN  LK QYL C+E+Y+E + DIE AK++L  GD++GV
Sbjct: 78  LNLTNTNAEKSLNLVKSLAATTTNPQLKNQYLSCYESYEEVVGDIEIAKSNLASGDFNGV 137

Query: 130 NLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSIL 179
           N+A S  M   DDC DSFKQP KD S+L  N K L D+ SIILVIS++L
Sbjct: 138 NIATSGIMTAVDDCQDSFKQPPKDTSMLLKNGKTLNDVCSIILVISNLL 184

BLAST of Csa6G307360 vs. NCBI nr
Match: gi|659113905|ref|XP_008456811.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 169.9 bits (429), Expect = 4.2e-39
Identity = 87/154 (56.49%), Postives = 109/154 (70.78%), Query Frame = 1

Query: 28  TILSNI---DTLASNICPQTSNPSFCASILENANNIDLKALIAYSLKLAHTNAGKSMTLA 87
           TI+SN+   + + S ICP+TSNPSFC+S+L++A   DLK L  Y+L LAHTNA KS+TLA
Sbjct: 19  TIISNVASSNDVVSTICPKTSNPSFCSSVLKSAGTTDLKGLAVYTLNLAHTNAHKSLTLA 78

Query: 88  KALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCH 147
           K LA  TTN  LK++Y  C E+YDE + DIE A+ DL  GD++ VN+  S AM   DDC 
Sbjct: 79  KLLATTTTNPQLKQRYSSCAESYDETVGDIENAQKDLALGDFNAVNIVTSGAMTEIDDCQ 138

Query: 148 DSFKQPLKDMSLLPNNTKMLKDIYSIILVISSIL 179
           D F QP KD SLL  N K L DIY+IILVIS++L
Sbjct: 139 DKFAQPPKDTSLLLKNGKTLNDIYNIILVISNLL 172

BLAST of Csa6G307360 vs. NCBI nr
Match: gi|659127944|ref|XP_008463970.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 169.1 bits (427), Expect = 7.2e-39
Identity = 88/154 (57.14%), Postives = 110/154 (71.43%), Query Frame = 1

Query: 28  TILSNI---DTLASNICPQTSNPSFCASILENANNIDLKALIAYSLKLAHTNAGKSMTLA 87
           TI+SN+   + + S ICP+TSNP FC+S+L++A   DLK L  Y+L LAHTNA KS+TLA
Sbjct: 19  TIISNVASSNDVISIICPKTSNPPFCSSVLKSAGTTDLKGLAVYTLNLAHTNACKSLTLA 78

Query: 88  KALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCH 147
           K+LA  TTN  LK++Y  CFE+YDEA+ DIE A+ DL  GD++ VN+  S AM   DDC 
Sbjct: 79  KSLATTTTNPQLKQRYSSCFESYDEAVGDIENAQKDLALGDFNAVNIVTSGAMTEIDDCQ 138

Query: 148 DSFKQPLKDMSLLPNNTKMLKDIYSIILVISSIL 179
           D F QP KD SLL  N K L DI SIILVIS++L
Sbjct: 139 DKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 172

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PMEI_ACTDE1.2e-1732.97Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2[more]
PMEI1_ARATH3.1e-1334.69Pectinesterase inhibitor 1 OS=Arabidopsis thaliana GN=PMEI1 PE=1 SV=1[more]
PMEI2_ARATH6.4e-1129.66Pectinesterase inhibitor 2 OS=Arabidopsis thaliana GN=PMEI2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KCC4_CUCSA2.0e-96100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307360 PE=4 SV=1[more]
A0A0A0KEW6_CUCSA1.6e-3752.15Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307350 PE=4 SV=1[more]
A0A0A0KCV8_CUCSA8.9e-3653.38Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307340 PE=4 SV=1[more]
A0A0A0KHY0_CUCSA6.0e-1634.76Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307370 PE=4 SV=1[more]
A0A072TNA9_MEDTR4.3e-1434.25Plant invertase/pectin methylesterase inhibitor OS=Medicago truncatula GN=MTR_8g... [more]
Match NameE-valueIdentityDescription
AT1G48020.11.7e-1434.69 pectin methylesterase inhibitor 1[more]
AT3G17220.13.6e-1229.66 pectin methylesterase inhibitor 2[more]
AT3G05741.18.6e-0623.76 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
Match NameE-valueIdentityDescription
gi|700192171|gb|KGN47375.1|2.9e-96100.00hypothetical protein Csa_6G307360 [Cucumis sativus][more]
gi|659117597|ref|XP_008458685.1|7.4e-7686.21PREDICTED: pectinesterase inhibitor-like [Cucumis melo][more]
gi|659117595|ref|XP_008458684.1|6.6e-4053.85PREDICTED: pectinesterase inhibitor-like [Cucumis melo][more]
gi|659113905|ref|XP_008456811.1|4.2e-3956.49PREDICTED: pectinesterase inhibitor-like [Cucumis melo][more]
gi|659127944|ref|XP_008463970.1|7.2e-3957.14PREDICTED: pectinesterase inhibitor-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G307360.1Csa6G307360.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 38..179
score: 1.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 38..173
score: 2.9
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 32..174
score: 7.7
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 7..177
score: 1.4
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 38..179
score: 7.06

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None