Csa6G307340 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa6G307340
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Pectinesterase inhibitor; contains IPR006501 (Pectinesterase inhibitor)
Location	Chr6 : 14669771 .. 14670289 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAATAACTCTTGTTTCATTACTGTCTCTCTCATTGGAGTTCTTTTGTTCATCATCATTTCATATGTGGCATCATCTAACGATGTCATTTCCACCATTTGTCCAAAAACCTCGAATCCGCCATTTTGTTCAAGTGTGTTGAAATCCGCAGGCACTACAGATCTGAAAGGCTTGGTTGTATACACCTTAAACCTTGCCCATACAAATGCTGGCAAATCTTTGACCCTAGCCAACTCATTGGCAAAAACCGCCACTAATCCTCAACTTAAGCAACGATATTCATCTTGTGTTGAGAGCTATGATGAAGCTGTTGGTGATATTGAAAATGCCCAAAAGGACTTAGCAGTTGAGGACTTTAATGGTGTCAATATTGTAACTTCTGGTGCCATGACGAATATTGGTGATTGTCAAGATAAGTTCGCTCAGCCACCGAAGGATACATCATTGCTTTTGGAGAATGGCAAGACTATAAATGATATATGCAGTATTATTTTGGTTATATCCAATCTTTTTTGA

mRNA sequence

ATGGCCAATAACTCTTGTTTCATTACTGTCTCTCTCATTGGAGTTCTTTTGTTCATCATCATTTCATATGTGGCATCATCTAACGATGTCATTTCCACCATTTGTCCAAAAACCTCGAATCCGCCATTTTGTTCAAGTGTGTTGAAATCCGCAGGCACTACAGATCTGAAAGGCTTGGTTGTATACACCTTAAACCTTGCCCATACAAATGCTGGCAAATCTTTGACCCTAGCCAACTCATTGGCAAAAACCGCCACTAATCCTCAACTTAAGCAACGATATTCATCTTGTGTTGAGAGCTATGATGAAGCTGTTGGTGATATTGAAAATGCCCAAAAGGACTTAGCAGTTGAGGACTTTAATGGTGTCAATATTGTAACTTCTGGTGCCATGACGAATATTGGTGATTGTCAAGATAAGTTCGCTCAGCCACCGAAGGATACATCATTGCTTTTGGAGAATGGCAAGACTATAAATGATATATGCAGTATTATTTTGGTTATATCCAATCTTTTTTGA

Coding sequence (CDS)

ATGGCCAATAACTCTTGTTTCATTACTGTCTCTCTCATTGGAGTTCTTTTGTTCATCATCATTTCATATGTGGCATCATCTAACGATGTCATTTCCACCATTTGTCCAAAAACCTCGAATCCGCCATTTTGTTCAAGTGTGTTGAAATCCGCAGGCACTACAGATCTGAAAGGCTTGGTTGTATACACCTTAAACCTTGCCCATACAAATGCTGGCAAATCTTTGACCCTAGCCAACTCATTGGCAAAAACCGCCACTAATCCTCAACTTAAGCAACGATATTCATCTTGTGTTGAGAGCTATGATGAAGCTGTTGGTGATATTGAAAATGCCCAAAAGGACTTAGCAGTTGAGGACTTTAATGGTGTCAATATTGTAACTTCTGGTGCCATGACGAATATTGGTGATTGTCAAGATAAGTTCGCTCAGCCACCGAAGGATACATCATTGCTTTTGGAGAATGGCAAGACTATAAATGATATATGCAGTATTATTTTGGTTATATCCAATCTTTTTTGA

Protein sequence

MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLVVYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNLF*

Homology

[Close]

BLAST of Csa6G307340 vs. Swiss-Prot
Match: PMEI_ACTDE (Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 2.5e-25
Identity = 66/174 (37.93%), Postives = 98/174 (56.32%), Query Frame = 1


Query: 5   SCFITVSLIGVLLFIII----SYVASSNDVISTICPKTSNPPFCSSVLKS---AGTTDLK 64
           S  + VSL+ V+LFI        V + N +IS ICPKT NP  C   L+S   + + DLK
Sbjct: 7   SSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLK 66

Query: 65  GLVVYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAV 124
           GL  +++++A  +A ++  +  SL   AT+P+LK RY +C E+Y +A+  +  A++ L  
Sbjct: 67  GLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTS 126

Query: 125 EDFNGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
            D+N +NI  S A    G C+D F  PP   + L +    + D+C I+LVISNL
Sbjct: 127 GDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNL 180

[Close]

BLAST of Csa6G307340 vs. Swiss-Prot
Match: PMEI2_ARATH (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 7.1e-20
Identity = 58/165 (35.15%), Postives = 84/165 (50.91%), Query Frame = 1


Query: 10  VSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTT---DLKGLVVYTLNL 69
           V +  ++ F++   + +    I  IC K  N  FC+S +KS   T   DL+ L   T   
Sbjct: 9   VLMSSLMFFVMTGSLNAQVADIKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGS 68

Query: 70  AHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNGVNIV 129
           A T+A +      SL KTATNP +K+ Y+SCV+ Y  A+  + +A++ LA  D  G+NI 
Sbjct: 69  AQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIK 128

Query: 130 TSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
            S AM     C+   A    D S  ++N     +IC I+LVISN+
Sbjct: 129 VSAAMEGPSTCEQDMADFKVDPS-AVKNSGDFQNICGIVLVISNM 172

[Close]

BLAST of Csa6G307340 vs. Swiss-Prot
Match: PMEI1_ARATH (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.3e-18
Identity = 59/169 (34.91%), Postives = 89/169 (52.66%), Query Frame = 1


Query: 4   NSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSA-GTTDLKGLVVY 63
           N+ F+  S +  LL I  SY  +S+++ STIC KT NP FC   L +   + +L+ L   
Sbjct: 7   NNAFL--SSLMFLLLIGSSYAITSSEM-STICDKTLNPSFCLKFLNTKFASPNLQALAKT 66

Query: 64  TLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNG 123
           TL+     A ++L    S+     +P+ K  Y SCV+ Y+ A+G++E A + LA  D  G
Sbjct: 67  TLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMG 126

Query: 124 VNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
           +N+  S A+     C D   +     S ++ N KTI ++C I LVISN+
Sbjct: 127 MNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNM 172

[Close]

BLAST of Csa6G307340 vs. Swiss-Prot
Match: PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.3e-09
Identity = 44/164 (26.83%), Postives = 75/164 (45.73%), Query Frame = 1


Query: 17  LFIIISYVASSNDVISTICPKTS------NPPFCSSVLKS---AGTTDLKGLVVYTLNLA 76
           L +++  V S+ D++   C K +      N  FC   L +   + T DL+GL V + NLA
Sbjct: 14  LLLLLQAVISA-DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLA 73

Query: 77  HTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNGVNIVT 136
             +  K  T    + K+  +P LK+  + CV  Y +A   ++ A  D   +D+   N+  
Sbjct: 74  IQHGSKIQTFIGRILKSKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKM 133

Query: 137 SGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
           S A+ +   C+D F +     S + +  K    + +I L I+ L
Sbjct: 134 SAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of Csa6G307340 vs. TrEMBL
Match: A0A0A0KCV8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307340 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 2.7e-90
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 1


Query: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60
           MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV
Sbjct: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60

Query: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120
           VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF
Sbjct: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120

Query: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNLF 173
           NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNLF
Sbjct: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNLF 172

[Close]

BLAST of Csa6G307340 vs. TrEMBL
Match: A0A0A0KEW6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307350 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.3e-46
Identity = 104/175 (59.43%), Postives = 123/175 (70.29%), Query Frame = 1


Query: 3   NNSCFITVSLIGVLLFIIISYVAS------SNDVISTICPKTSNPPFCSSVLKSAGTTDL 62
           N   F +++++ V  F+  S V S      SNDV S+ICPKT NPPFC  VLKSAG+TDL
Sbjct: 7   NVRIFGSIAIVFVFSFLFFSVVPSRATTTPSNDVASSICPKTRNPPFCVDVLKSAGSTDL 66

Query: 63  KGLVVYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLA 122
           K L  YTLNLA+ NA KS  LA SLA   TNP LK +Y SC ESY+EA  DIENA+ +LA
Sbjct: 67  KVLATYTLNLANENALKSTNLAKSLAAMTTNPPLKNQYLSCYESYEEATSDIENAKSNLA 126

Query: 123 VEDFNGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
             DFNGVNI TSG MT++ DC D F Q   D S LL++GKT+ND+CSIILVISNL
Sbjct: 127 SGDFNGVNIATSGVMTSVSDCLDSFKQLRIDPS-LLKDGKTLNDVCSIILVISNL 180

[Close]

BLAST of Csa6G307340 vs. TrEMBL
Match: A0A0A0KCC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307360 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 8.4e-36
Identity = 79/148 (53.38%), Postives = 104/148 (70.27%), Query Frame = 1


Query: 24  VASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLVVYTLNLAHTNAGKSLTLANSLAK 83
           +++ + + S ICP+TSNP FC+S+L++A   DLK L+ Y+L LAHTNAGKS+TLA +LA 
Sbjct: 30  LSNIDTLASNICPQTSNPSFCASILENANNIDLKALIAYSLKLAHTNAGKSMTLAKALAV 89

Query: 84  TATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNGVNIVTSGAMTNIGDCQDKFAQ 143
             TN  LK++Y  C E+YDEA+ DIE A+ DL   D++GVN+  S AM    DC D F Q
Sbjct: 90  LTTNTLLKKQYLFCFENYDEAMCDIEKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQ 149

Query: 144 PPKDTSLLLENGKTINDICSIILVISNL 172
           P KD SLL  N K + DI SIILVIS++
Sbjct: 150 PLKDMSLLPNNTKMLKDIYSIILVISSI 177

[Close]

BLAST of Csa6G307340 vs. TrEMBL
Match: A0A072TNA9_MEDTR (Plant invertase/pectin methylesterase inhibitor OS=Medicago truncatula GN=MTR_8g032810 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.4e-21
Identity = 62/169 (36.69%), Postives = 99/169 (58.58%), Query Frame = 1


Query: 10  VSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKS---AGTTDLKGLVVYTLNL 69
           +S++ V L  + S  A++   +  IC K +NP FCS++L+S       DL  L  YT+++
Sbjct: 5   LSIMVVFLACVASSYATNVVNVQEICKKATNPSFCSTILESKPGGAGKDLVSLASYTIDV 64

Query: 70  AHTNAGKSLTLANSLAKTATNPQLKQ-RYSSCVESY---DEAVGDIENAQKDLAVEDFNG 129
             TN   +++L   LA  + +  LKQ RY +C+  +   D A+G++E AQK L   D+NG
Sbjct: 65  LRTNVSNTISLITKLAAQSGSDSLKQNRYKNCLSRFGMDDGALGEVEEAQKVLKDSDYNG 124

Query: 130 VNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
           VN+  +  MT + +C  K + P  DTSLL ++ + +N I  IIL+ISN+
Sbjct: 125 VNMHITSVMTAVDECLSKDSSPDNDTSLLPKDVECVNQIAQIILIISNV 173

[Close]

BLAST of Csa6G307340 vs. TrEMBL
Match: V4LWJ6_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10022367mg PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.5e-21
Identity = 64/165 (38.79%), Postives = 91/165 (55.15%), Query Frame = 1


Query: 10  VSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTT---DLKGLVVYTLNL 69
           V +  ++ F++I  + +    I  IC K  NP FCSS +KS   T   DL+ L   T   
Sbjct: 9   VFVSSLMFFVMIGSLNAQVADIKAICGKAKNPSFCSSYMKSNPKTSGADLRTLANITFGS 68

Query: 70  AHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNGVNIV 129
           A T+A  +L    SL KT TNP LK+ Y+SCVE Y  ++ ++ +A+++LA  D  G+NI 
Sbjct: 69  AQTSAKGALAKIQSLVKTETNPTLKKGYTSCVEQYKNSISNLNDAKQNLASGDGPGLNIK 128

Query: 130 TSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
            S AM     CQD  A    D S  ++N     +IC I+LVISN+
Sbjct: 129 VSAAMEGSSTCQDNLANVKADPS-TVKNSGDFQNICGIVLVISNM 172

[Close]

BLAST of Csa6G307340 vs. TAIR10
Match: AT3G17220.1 (AT3G17220.1 pectin methylesterase inhibitor 2)

HSP 1 Score: 98.6 bits (244), Expect = 4.0e-21
Identity = 58/165 (35.15%), Postives = 84/165 (50.91%), Query Frame = 1


Query: 10  VSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTT---DLKGLVVYTLNL 69
           V +  ++ F++   + +    I  IC K  N  FC+S +KS   T   DL+ L   T   
Sbjct: 9   VLMSSLMFFVMTGSLNAQVADIKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGS 68

Query: 70  AHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNGVNIV 129
           A T+A +      SL KTATNP +K+ Y+SCV+ Y  A+  + +A++ LA  D  G+NI 
Sbjct: 69  AQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIK 128

Query: 130 TSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
            S AM     C+   A    D S  ++N     +IC I+LVISN+
Sbjct: 129 VSAAMEGPSTCEQDMADFKVDPS-AVKNSGDFQNICGIVLVISNM 172

[Close]

BLAST of Csa6G307340 vs. TAIR10
Match: AT1G48020.1 (AT1G48020.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 94.4 bits (233), Expect = 7.6e-20
Identity = 59/169 (34.91%), Postives = 89/169 (52.66%), Query Frame = 1


Query: 4   NSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSA-GTTDLKGLVVY 63
           N+ F+  S +  LL I  SY  +S+++ STIC KT NP FC   L +   + +L+ L   
Sbjct: 7   NNAFL--SSLMFLLLIGSSYAITSSEM-STICDKTLNPSFCLKFLNTKFASPNLQALAKT 66

Query: 64  TLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDFNG 123
           TL+     A ++L    S+     +P+ K  Y SCV+ Y+ A+G++E A + LA  D  G
Sbjct: 67  TLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMG 126

Query: 124 VNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
           +N+  S A+     C D   +     S ++ N KTI ++C I LVISN+
Sbjct: 127 MNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNM 172

[Close]

BLAST of Csa6G307340 vs. TAIR10
Match: AT3G36659.1 (AT3G36659.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 58.9 bits (141), Expect = 3.5e-09
Identity = 48/145 (33.10%), Postives = 59/145 (40.69%), Query Frame = 1


Query: 31  ISTICPKTSNPPFCSSVLKSAGTTDLKGLVVYTLNLAHTNAGKSLTLANSLAKTATNPQL 90
           I TIC KT NPP C S +    T  LK            N    L LA   + TAT   +
Sbjct: 130 IKTICGKTDNPPLCESSVSPLLTPQLK-----------PNTSSVLILAIQASITATKAAM 189

Query: 91  ----KQRYSSCVESYDEAVGDIENAQKDLAVEDFNGVNIVTSGAMTNIGDCQDKFAQPPK 150
               K   S C E YD+AV ++E+A   +   D   VN   S AMT+   C D F +  +
Sbjct: 190 AIVEKVDASDCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGE 249

Query: 151 DTSLLLENGKTINDICSIILVISNL 172
              L     K +  + S  L IS L
Sbjct: 250 PNPLAYVADK-LTKMVSNCLAISTL 262

[Close]

BLAST of Csa6G307340 vs. TAIR10
Match: AT3G05741.1 (AT3G05741.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 57.8 bits (138), Expect = 7.8e-09
Identity = 42/165 (25.45%), Postives = 71/165 (43.03%), Query Frame = 1


Query: 15  VLLFIIISYVASSNDVISTICPKTSNPPFCSSVL--------------KSAGTTDLKGLV 74
           +LL I+I   +SS+     I  K     FCS++               +   +  ++G +
Sbjct: 18  LLLIILIITPSSSSLSFKDIVTKELINNFCSNLEMFDRQFCAKWFNADQKTKSISVQGFI 77

Query: 75  VYTLNLAHTNAGKSLTLANSLAKTA-TNPQLKQRYSSCVESYDEAVGDIENAQKDLAVED 134
              +        ++  L + LAK++  + QLK  Y SCV SY +A+ ++E AQK L+   
Sbjct: 78  SLRVKETREFGLQTQALMSKLAKSSGKDQQLKGSYESCVASYGQAIKELEKAQKFLSSNS 137

Query: 135 FNGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSI 165
           F    +  S A    GDC+D F  P  +  L+    +   ++C +
Sbjct: 138 FTQALVAISDAFYKAGDCKDAFEGPSNEPPLVFNRVENFANMCYV 182

[Close]

BLAST of Csa6G307340 vs. TAIR10
Match: AT1G48010.1 (AT1G48010.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 57.0 bits (136), Expect = 1.3e-08
Identity = 38/125 (30.40%), Postives = 61/125 (48.80%), Query Frame = 1


Query: 52  GTTDLKGLVVYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENA 111
           G  DL G+    ++ A  NA  +      LA + T+ + K+ Y SC + YD+A+   + A
Sbjct: 54  GKLDLPGVAKCLIHYAQQNALDTHNQLQLLANSTTDNRTKESYISCSKYYDKALYSFDEA 113

Query: 112 QKDLAVEDFNGVNIVTSGAMTNIGDC-----QDKFAQPPKDTSLLLENGKTINDICSIIL 171
            KDL V + + +NI  + A  N  DC     +D F   PK    L+     + ++C I+L
Sbjct: 114 LKDLKVNNPDYLNIEITAARQNANDCKTLDLKDVFKPDPK----LIMKIDFLENVCGILL 173

[Close]

BLAST of Csa6G307340 vs. NCBI nr
Match: gi|778722211|ref|XP_004148234.2| (PREDICTED: pectinesterase inhibitor-like [Cucumis sativus])

HSP 1 Score: 339.3 bits (869), Expect = 3.8e-90
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 1


Query: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60
           MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV
Sbjct: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60

Query: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120
           VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF
Sbjct: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120

Query: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNLF 173
           NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNLF
Sbjct: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNLF 172

[Close]

BLAST of Csa6G307340 vs. NCBI nr
Match: gi|659127944|ref|XP_008463970.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 295.4 bits (755), Expect = 6.3e-77
Identity = 150/171 (87.72%), Postives = 155/171 (90.64%), Query Frame = 1


Query: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60
           M NNSC + VSLIGV+LF IIS VASSNDVIS ICPKTSNPPFCSSVLKSAGTTDLKGL 
Sbjct: 1   MTNNSCIVIVSLIGVILFTIISNVASSNDVISIICPKTSNPPFCSSVLKSAGTTDLKGLA 60

Query: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120
           VYTLNLAHTNA KSLTLA SLA T TNPQLKQRYSSC ESYDEAVGDIENAQKDLA+ DF
Sbjct: 61  VYTLNLAHTNACKSLTLAKSLATTTTNPQLKQRYSSCFESYDEAVGDIENAQKDLALGDF 120

Query: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
           N VNIVTSGAMT I DCQDKFAQPPKDTSLLL+NGKT+NDICSIILVISNL
Sbjct: 121 NAVNIVTSGAMTEIDDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNL 171

[Close]

BLAST of Csa6G307340 vs. NCBI nr
Match: gi|659087170|ref|XP_008444309.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 290.8 bits (743), Expect = 1.6e-75
Identity = 146/171 (85.38%), Postives = 154/171 (90.06%), Query Frame = 1


Query: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60
           MANNSC + VSLIGVLLF IIS VASSNDV+STICPKTSNPPFCSSVLKSAGTTDLKGL 
Sbjct: 1   MANNSCLVIVSLIGVLLFTIISNVASSNDVVSTICPKTSNPPFCSSVLKSAGTTDLKGLA 60

Query: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120
           VY LNLAHTNA +SLTLA SL  T TNPQLKQRYSSC +SYDEAVGDIEN QKDLA+ DF
Sbjct: 61  VYILNLAHTNARESLTLAKSLTTTTTNPQLKQRYSSCAKSYDEAVGDIENGQKDLALGDF 120

Query: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
           N VNIVTSGAMT I DCQDKF+QPPKDTSLLL+NGKT+NDICSIILVISNL
Sbjct: 121 NAVNIVTSGAMTEIDDCQDKFSQPPKDTSLLLKNGKTLNDICSIILVISNL 171

[Close]

BLAST of Csa6G307340 vs. NCBI nr
Match: gi|659128306|ref|XP_008464144.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 288.1 bits (736), Expect = 1.0e-74
Identity = 149/171 (87.13%), Postives = 153/171 (89.47%), Query Frame = 1


Query: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60
           M  NSC   VSLIGVLLF I   VASS DV+STICPKTSNP FCSSVLKSAGTTDLKGL 
Sbjct: 9   MVYNSCLFIVSLIGVLLFTI--NVASSTDVVSTICPKTSNPQFCSSVLKSAGTTDLKGLA 68

Query: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120
           VYTLNLAHTNA KSLTLANSLAKT TNPQLKQRYSSCVESYDEAVGDIEN QKDLA+ DF
Sbjct: 69  VYTLNLAHTNARKSLTLANSLAKTTTNPQLKQRYSSCVESYDEAVGDIENVQKDLALGDF 128

Query: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
           NGVNIVTSGAMT I DCQDKFAQPPKDTSLLL+NGKT+NDICSIILVISNL
Sbjct: 129 NGVNIVTSGAMTEIDDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNL 177

[Close]

BLAST of Csa6G307340 vs. NCBI nr
Match: gi|659113905|ref|XP_008456811.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 288.1 bits (736), Expect = 1.0e-74
Identity = 147/171 (85.96%), Postives = 153/171 (89.47%), Query Frame = 1


Query: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60
           MANNSC I VSL+GVLLF IIS VASSNDV+STICPKTSNP FCSSVLKSAGTTDLKGL 
Sbjct: 1   MANNSCLIIVSLVGVLLFTIISNVASSNDVVSTICPKTSNPSFCSSVLKSAGTTDLKGLA 60

Query: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120
           VYTLNLAHTNA KSLTLA  LA T TNPQLKQRYSSC ESYDE VGDIENAQKDLA+ DF
Sbjct: 61  VYTLNLAHTNAHKSLTLAKLLATTTTNPQLKQRYSSCAESYDETVGDIENAQKDLALGDF 120

Query: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 172
           N VNIVTSGAMT I DCQDKFAQPPKDTSLLL+NGKT+NDI +IILVISNL
Sbjct: 121 NAVNIVTSGAMTEIDDCQDKFAQPPKDTSLLLKNGKTLNDIYNIILVISNL 171

The following BLAST results are available for this feature:

BLAST of Csa6G307340 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PMEI_ACTDE	2.5e-25	37.93	Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2	[more]
PMEI2_ARATH	7.1e-20	35.15	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana GN=PMEI2 PE=1 SV=1	[more]
PMEI1_ARATH	1.3e-18	34.91	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana GN=PMEI1 PE=1 SV=1	[more]
PLA1_PLAAC	4.3e-09	26.83	Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1	[more]

BLAST of Csa6G307340 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCV8_CUCSA	2.7e-90	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307340 PE=4 SV=1	[more]
A0A0A0KEW6_CUCSA	5.3e-46	59.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307350 PE=4 SV=1	[more]
A0A0A0KCC4_CUCSA	8.4e-36	53.38	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307360 PE=4 SV=1	[more]
A0A072TNA9_MEDTR	3.4e-21	36.69	Plant invertase/pectin methylesterase inhibitor OS=Medicago truncatula GN=MTR_8g...	[more]
V4LWJ6_EUTSA	4.5e-21	38.79	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10022367mg PE=4 SV=1	[more]

BLAST of Csa6G307340 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G17220.1	4.0e-21	35.15	pectin methylesterase inhibitor 2	[more]
AT1G48020.1	7.6e-20	34.91	pectin methylesterase inhibitor 1	[more]
AT3G36659.1	3.5e-09	33.10	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G05741.1	7.8e-09	25.45	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G48010.1	1.3e-08	30.40	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Csa6G307340 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778722211\|ref\|XP_004148234.2\|	3.8e-90	100.00	PREDICTED: pectinesterase inhibitor-like [Cucumis sativus]	[more]
gi\|659127944\|ref\|XP_008463970.1\|	6.3e-77	87.72	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]
gi\|659087170\|ref\|XP_008444309.1\|	1.6e-75	85.38	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]
gi\|659128306\|ref\|XP_008464144.1\|	1.0e-74	87.13	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]
gi\|659113905\|ref\|XP_008456811.1\|	1.0e-74	85.96	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU143258	cucumber EST collection version 3.0	transcribed_cluster
CU166831	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa6G307340.1	Csa6G307340.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU166831	CU166831	transcribed_cluster
CU143258	CU143258	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 28..172 score: 2.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..168 score: 3.5
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..168 score: 3.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 11..170 score: 2.3
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..172 score: 3.01
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 1..172 score: 1.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa6G307340	Cla009245	Watermelon (97103) v1	cuwmB538
Csa6G307340	MELO3C021269	Melon (DHL92) v3.5.1	cumeB445
Csa6G307340	ClCG06G004590	Watermelon (Charleston Gray)	cuwcgB477
Csa6G307340	Lsi09G014880	Bottle gourd (USVL1VR-Ls)	culsiB403
Csa6G307340	Cla97C06G114010	Watermelon (97103) v2	cuwmbB469
Csa6G307340	Bhi12G001726	Wax gourd	cuwgoB547
Csa6G307340	CSPI06G16930	Wild cucumber (PI 183967)	cpicuB313
Csa6G307340	Cucsa.316130	Cucumber (Gy14) v1	cgycuB454
Csa6G307340	Cp4.1LG12g03240	Cucurbita pepo (Zucchini)	cpecuB151
Csa6G307340	CsGy6G016460	Cucumber (Gy14) v2	cgybcuB281

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa6G307340	Cucurbita maxima (Rimu)	cmacuB230
Csa6G307340	Melon (DHL92) v3.6.1	cumedB426
Csa6G307340	Silver-seed gourd	carcuB0078