Csa6G181540 (gene) Cucumber (Chinese Long) v2

NameCsa6G181540
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionGlucan endo-1,3-beta-glucosidase; contains IPR000490 (Glycoside hydrolase, family 17), IPR012946 (X8), IPR017853 (Glycoside hydrolase, superfamily)
LocationChr6 : 11773719 .. 11776714 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACATTCCACATTTCAGTGTGGATCTCTCTCTCACTCTCCCTCTCCCTCTCCAGTAATGCCTCCGACTCTTTCCCTCATTCTCTTCTTCTTCTTCCTCTTCCTTAATCTCTCCGCCTTAACCTTTTCTCCCGTCTCCGCCCTCGGCTTCAACTGGGGAACCTCCGCGTCCCACCCTCTTTCTCCTTTCAAGGTTGTCCAACTCTTGAACTCCAACAACATCACCAAAATCAAGCTCCTCGATCCCAATCCCCTCGTCCTCCAGGCCTTATCTGCCTCCACTCTTCAACTCACCATCGCCATTCCCAATCCCATGCTCAAACTCCTAAACTCTTCCCGAAAAGCTGCCGAAAGTTGGGTCCACGATAATGTCACTAGGTATCTCTCCGGCGGTACAGGCGGTGTTCGAATCGAGTACGCTTCTTTAACTCCTTCTCTTTCTCTTAATCAACTAATTCATTCTCATTTAGAGATTAAACGTCGTATTCACTATTAGTTCGCATGGCTGTTAACAGTGCTTCAACTAACGACTGAATTTATTACAAACAGTTTCCTGCCCAATTTCAAAGCCTGCATAATCAATGACCGTAGATATCAGGAATAGGTTAGCACTATTGATACGAAGTACATAATGCTGGCCCATTTTCATTTTGGGAAGAACAAATCTTTTGCAACTATAATTTAATTCAGCTAGAGATCTTGCACTAACAAATGAATTTTACACCCTAAATTTGGAAAGGGTGTCTCGAACAATTTCAATGATGGTGTTGATGTTCCCTGTGCAATTAATGCTATAACCTATAGACAATAATACCTATACCGAATTGTTATGTATTAAAGAAACTCCATAATTTCACATGTTTGGTGATTTACTTATGCTCTGTTCTGTGGTGGGTGCATGGCTGAATTAGAAAAAATTTTGTGTAGCATTTTTCATGCGATGTGCTTGGAAATGGAAGGCTCTATTGGATATGATGAGGGTGCCCGGATGCACCTTCTGATCGGCTAATCCTATTAATATTGTACTTTGTACTTCATTTTTACTACATTAATGAAATCTTTGTTTCCGTTTCAAAAAATGAAGAAAAAAAAAAGAAAGCTGTATACAAACTGTCTCGAACCATTTTTTTTTTATATAATGGATGCCTCTGTCAAACAAATTTACTCTAAATCTAGACATGAAGTAGGTTACATAGTGGCAGGGCAAGATGGTAGTCGAGGGTACATTTTTGGGGTCTGTCGCTTGTTAGGAGCAAGGAACTACCAACTGTTTTCCGTTTTCCCACTCTTAGAAGATGAATTTCTAGGACAACGTTCTGATGTTGTCGTGGCTGCCAACTGTTCCCTCTTCCTCTTTACTATAGTGTTGTTAGAACAAACTATTGCTTGATTTGCTTAGCAATATTTGAGTAACGATTCAGTCAGTTCTAGTCAGCTAAGTGAGAACTAATAAATTGTTGGATGAGTGTAGGTATTAGATTCGTGACTGGTGAGTCATCTCATGTTAGCAAATGATTTCTATCTCTCTAGGAAACTTTGAAGAAGCACCAAAGTCTTTAATATCCATTTGATTGGAATGTCCATTCACTTTCTCTCTGATGTTTGCTATTATGCTTCAGGTATATTGCAGTTGGAGATGAACCATTTCTTCTAAGTTATGGGGACGAGTATTATCCGTATGTCATGGGAGCAGTTGCCAACATTCAGGCAGCTATAACCAAGGTGAACTTGGAGAGCAGGATAAAAGTTGTTGTCCCATGCAGTTACGACGCATTCCTGTCTGAATCTGGCCTTCCTTCAAAGGGACATTTCCGACCTGAATTGAATAAAACAATGATTCAGCTCCTGACATTTCTTACTGCACACAGATCACCGTTCTTTGCATCCATTTCCCCATTTTTAAGTTTCCTTCAAAACAAAAATATTTCTCTGGACTTTGCCCTCTTCAAGGAAACAGCACGCCCCCATAACGATAGCCATAGAACTTACAAAAACAGCTTTGAGTTGAGTTATGATACCTTAGTTGCATCTTTATCAAAGATTGGATTTTCGACAGTGGATATCGTTGTGGAACAGGTTGGATGGCCTACTGATGGAGCAGACAATGCTAACTCATCAACAGCTGAAACCTTTATGAAAGGTTTGCTGGATTACCTCCATAGCAGATCAGGGTCCCCCCTTAGACCTCGACGTCCACCTCTTGAAACCTATATCTTAAGCCTATTAGATGAAGATCGGAGAAATATATCCACTGGACCATTTGAGAGACACTGGGGAGTGTTCACTTTTGATGGCCAAGCAAAGTACCACCTTAACTTTGGTCAGAGAACAAAAAATCTTGTGAATGCTCAAAATGTAGAATACCTCTCCCCCAAGTGGTGTGTTCTCAACAACAACAAAGACTTATCTAATGCAAGTGCTCGTGCTTTAGAGGCATGTTCTGTCGCTGACTGCACTGCGCTTGCTCCTGGTGCATCTTGCTCCAATATTGGTTGGCCTGGTAATATCTCTTATGCATTCAATAGCTACTATCAACAAAATGATCAAAGACCAGAAAGTTGTGACTTTCAAGGGTTGGCTTTGATCACAACCATCGATCCATCAAACAACAACTGCAGATTTCCCGTCCAAATTCGAACCTCGAATTCACAATCACTCGATGCTTCCTTCCTTCTAAAGGCAATCCCATTATCAGCAGTCTTTTGGTTATCTCAAATTGTTTATCTACAATAGAGCAATGGGGATGCACTCCATTTTGAAAATGAGTCGTTTTCCGAGTTTGGTAAAGCTCTAGATTTCTTGGTATGTTTCCTTTCTCCATTATGTTTCCAATTCAAGTGGGGAAAAGTAAGCATGTTTTCCAATGTATATCTAAAATGTGAAGCTAACCTATGTGTAATGTTTGAGATTGGAGAGACTAACATGAACCCTTTTGTTATTATTGTCTAACAAAAACACATTCCCATTTACTTCCTTACATGGCCTCATTTTTCCCTGC

mRNA sequence

ATGCCTCCGACTCTTTCCCTCATTCTCTTCTTCTTCTTCCTCTTCCTTAATCTCTCCGCCTTAACCTTTTCTCCCGTCTCCGCCCTCGGCTTCAACTGGGGAACCTCCGCGTCCCACCCTCTTTCTCCTTTCAAGGTTGTCCAACTCTTGAACTCCAACAACATCACCAAAATCAAGCTCCTCGATCCCAATCCCCTCGTCCTCCAGGCCTTATCTGCCTCCACTCTTCAACTCACCATCGCCATTCCCAATCCCATGCTCAAACTCCTAAACTCTTCCCGAAAAGCTGCCGAAAGTTGGGTCCACGATAATGTCACTAGGTATCTCTCCGGCGGTACAGGCGGTGTTCGAATCGAGTATATTGCAGTTGGAGATGAACCATTTCTTCTAAGTTATGGGGACGAGTATTATCCGTATGTCATGGGAGCAGTTGCCAACATTCAGGCAGCTATAACCAAGGTGAACTTGGAGAGCAGGATAAAAGTTGTTGTCCCATGCAGTTACGACGCATTCCTGTCTGAATCTGGCCTTCCTTCAAAGGGACATTTCCGACCTGAATTGAATAAAACAATGATTCAGCTCCTGACATTTCTTACTGCACACAGATCACCGTTCTTTGCATCCATTTCCCCATTTTTAAGTTTCCTTCAAAACAAAAATATTTCTCTGGACTTTGCCCTCTTCAAGGAAACAGCACGCCCCCATAACGATAGCCATAGAACTTACAAAAACAGCTTTGAGTTGAGTTATGATACCTTAGTTGCATCTTTATCAAAGATTGGATTTTCGACAGTGGATATCGTTGTGGAACAGGTTGGATGGCCTACTGATGGAGCAGACAATGCTAACTCATCAACAGCTGAAACCTTTATGAAAGGTTTGCTGGATTACCTCCATAGCAGATCAGGGTCCCCCCTTAGACCTCGACGTCCACCTCTTGAAACCTATATCTTAAGCCTATTAGATGAAGATCGGAGAAATATATCCACTGGACCATTTGAGAGACACTGGGGAGTGTTCACTTTTGATGGCCAAGCAAAGTACCACCTTAACTTTGGTCAGAGAACAAAAAATCTTGTGAATGCTCAAAATGTAGAATACCTCTCCCCCAAGTGGTGTGTTCTCAACAACAACAAAGACTTATCTAATGCAAGTGCTCGTGCTTTAGAGGCATGTTCTGTCGCTGACTGCACTGCGCTTGCTCCTGGTGCATCTTGCTCCAATATTGGTTGGCCTGGTAATATCTCTTATGCATTCAATAGCTACTATCAACAAAATGATCAAAGACCAGAAAGTTGTGACTTTCAAGGGTTGGCTTTGATCACAACCATCGATCCATCAAACAACAACTGCAGATTTCCCGTCCAAATTCGAACCTCGAATTCACAATCACTCGATGCTTCCTTCCTTCTAAAGGCAATCCCATTATCAGCAGTCTTTTGGTTATCTCAAATTGTTTATCTACAATAG

Coding sequence (CDS)

ATGCCTCCGACTCTTTCCCTCATTCTCTTCTTCTTCTTCCTCTTCCTTAATCTCTCCGCCTTAACCTTTTCTCCCGTCTCCGCCCTCGGCTTCAACTGGGGAACCTCCGCGTCCCACCCTCTTTCTCCTTTCAAGGTTGTCCAACTCTTGAACTCCAACAACATCACCAAAATCAAGCTCCTCGATCCCAATCCCCTCGTCCTCCAGGCCTTATCTGCCTCCACTCTTCAACTCACCATCGCCATTCCCAATCCCATGCTCAAACTCCTAAACTCTTCCCGAAAAGCTGCCGAAAGTTGGGTCCACGATAATGTCACTAGGTATCTCTCCGGCGGTACAGGCGGTGTTCGAATCGAGTATATTGCAGTTGGAGATGAACCATTTCTTCTAAGTTATGGGGACGAGTATTATCCGTATGTCATGGGAGCAGTTGCCAACATTCAGGCAGCTATAACCAAGGTGAACTTGGAGAGCAGGATAAAAGTTGTTGTCCCATGCAGTTACGACGCATTCCTGTCTGAATCTGGCCTTCCTTCAAAGGGACATTTCCGACCTGAATTGAATAAAACAATGATTCAGCTCCTGACATTTCTTACTGCACACAGATCACCGTTCTTTGCATCCATTTCCCCATTTTTAAGTTTCCTTCAAAACAAAAATATTTCTCTGGACTTTGCCCTCTTCAAGGAAACAGCACGCCCCCATAACGATAGCCATAGAACTTACAAAAACAGCTTTGAGTTGAGTTATGATACCTTAGTTGCATCTTTATCAAAGATTGGATTTTCGACAGTGGATATCGTTGTGGAACAGGTTGGATGGCCTACTGATGGAGCAGACAATGCTAACTCATCAACAGCTGAAACCTTTATGAAAGGTTTGCTGGATTACCTCCATAGCAGATCAGGGTCCCCCCTTAGACCTCGACGTCCACCTCTTGAAACCTATATCTTAAGCCTATTAGATGAAGATCGGAGAAATATATCCACTGGACCATTTGAGAGACACTGGGGAGTGTTCACTTTTGATGGCCAAGCAAAGTACCACCTTAACTTTGGTCAGAGAACAAAAAATCTTGTGAATGCTCAAAATGTAGAATACCTCTCCCCCAAGTGGTGTGTTCTCAACAACAACAAAGACTTATCTAATGCAAGTGCTCGTGCTTTAGAGGCATGTTCTGTCGCTGACTGCACTGCGCTTGCTCCTGGTGCATCTTGCTCCAATATTGGTTGGCCTGGTAATATCTCTTATGCATTCAATAGCTACTATCAACAAAATGATCAAAGACCAGAAAGTTGTGACTTTCAAGGGTTGGCTTTGATCACAACCATCGATCCATCAAACAACAACTGCAGATTTCCCGTCCAAATTCGAACCTCGAATTCACAATCACTCGATGCTTCCTTCCTTCTAAAGGCAATCCCATTATCAGCAGTCTTTTGGTTATCTCAAATTGTTTATCTACAATAG

Protein sequence

MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVFWLSQIVYLQ*
BLAST of Csa6G181540 vs. Swiss-Prot
Match: E139_ARATH (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana GN=At5g58480 PE=1 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 1.7e-175
Identity = 310/475 (65.26%), Postives = 370/475 (77.89%), Query Frame = 1

Query: 12  FFLFLNLSA---LTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVL 71
           F L L ++A   LT + V A+G NWGT ASHPL P KVV+LL SN I K+KL D +P VL
Sbjct: 6   FLLLLAVTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVL 65

Query: 72  QALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPF 131
           +ALS S + +TI I N MLK LN+S K AESWVHDNVTRY +GG   VRIEY+AVG+EPF
Sbjct: 66  RALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRYFNGGNR-VRIEYVAVGEEPF 125

Query: 132 LLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELN 191
           L SYG++Y P+V+GA  NIQ A+ K NL + +KVVVP S+D+FLSESG PS GHFR +LN
Sbjct: 126 LQSYGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLN 185

Query: 192 KTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFEL 251
           KTMI+LL+FLT H SPFF +ISPFLSF QNKNISLDF+LFKETA+ H D  +TY+NSF+L
Sbjct: 186 KTMIELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDL 245

Query: 252 SYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRP 311
           SYDTLV++L  IGFS VDIVV ++GWPTDGA+NA S TAE F KGL+ +L  ++ S    
Sbjct: 246 SYDTLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTAS---L 305

Query: 312 RRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYL 371
            RPP+ETYI SLLDED+RN+S G FERHWGVFTFDGQAKY+ +F    KN VNAQNV+YL
Sbjct: 306 PRPPVETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNH--KNQVNAQNVQYL 365

Query: 372 SPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQ 431
            PKWCV+NNNKDLSNASARALEAC+VADCT++ PG SCS I WPGN+SYAFNS YQQND 
Sbjct: 366 PPKWCVVNNNKDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDH 425

Query: 432 RPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVFWLS 484
             ESC+F GL LITT+DPS +NCRF +Q+ TS+S S   +F  ++ PL  +F LS
Sbjct: 426 SAESCNFGGLGLITTVDPSEDNCRFSIQLDTSHSSSQTPNF-FQSWPLLLLFLLS 473

BLAST of Csa6G181540 vs. Swiss-Prot
Match: E136_ARATH (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana GN=At5g58090 PE=1 SV=2)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 194/447 (43.40%), Postives = 265/447 (59.28%), Query Frame = 1

Query: 14  LFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQALSA 73
           L L   AL     S++G NWGT ASHPL P  VV++L  N I K+KL D     L+AL  
Sbjct: 7   LLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRALGK 66

Query: 74  STLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFLLSYG 133
           S +++ + IPN ML  L SS KAAE WV  NV+ ++S  T  V I Y+AVG+EPFL +Y 
Sbjct: 67  SGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHIS--TDNVNIRYVAVGNEPFLSTYN 126

Query: 134 DEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNKTMIQ 193
             Y      A+ NIQ AI K  L++++KV  P + D + S +  PS G FR  +   MI 
Sbjct: 127 GSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANIRDLMIT 186

Query: 194 LLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELSYDTL 253
           ++ FL+ +  PF  +I P++S   N +  +D+A F   A+P ND    Y N F+ +YDTL
Sbjct: 187 IVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYDTL 246

Query: 254 VASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPRRPPL 313
           V +L K GF  + I++ ++GWPTDG  NAN   A+ F +G + ++    G+P RP   P+
Sbjct: 247 VHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRP--GPI 306

Query: 314 ETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFG-QRTKNLVNAQNVEYLSPKW 373
           + Y+ SL+DED +++  G FERHWG+FTFDG  KY LN G   T  L+ A+ V YL  KW
Sbjct: 307 DAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGALIQAKGVRYLERKW 366

Query: 374 CVLNNNKDLSN--ASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQRP 433
           CV+  N  L +   +     ACS+ DCT+L  G SC+N+    NISYAFNSYYQ  DQ  
Sbjct: 367 CVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLD 426

Query: 434 ESCDFQGLALITTIDPSNNNCRFPVQI 458
            +C F  ++ +T  DPS   CRFP+ I
Sbjct: 427 TACKFPNISEVTKTDPSTGTCRFPIMI 449

BLAST of Csa6G181540 vs. Swiss-Prot
Match: E135_ARATH (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana GN=At4g31140 PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 195/476 (40.97%), Postives = 287/476 (60.29%), Query Frame = 1

Query: 12  FFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQAL 71
           FF+   L A     V  +G NWG+ A HPL P  VV+LL  N I K+KL + +  +L+AL
Sbjct: 10  FFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKAL 69

Query: 72  SASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFLLS 131
           S + +Q+ + IPN +L  L  S  AAE WV  NV+ ++S  + GV I Y+AVG+EPFL +
Sbjct: 70  SRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVS--SNGVDIRYVAVGNEPFLKA 129

Query: 132 YGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNKTM 191
           +   +    + A+ NIQ+AI K  L +++KV VP + D + S S LPS G FRPE+   M
Sbjct: 130 FNGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLM 189

Query: 192 IQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELSYD 251
           + ++ FL+ + +PF  +I PF+S   + N  ++FA F  T  P ND+ R Y N  + +YD
Sbjct: 190 LNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYD 249

Query: 252 TLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPRRP 311
           TLV SL K GF  + I+V +VGWPTDG  NAN   A  + +G ++   +  G+P+RP   
Sbjct: 250 TLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRP--G 309

Query: 312 PLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYLSPK 371
            ++ Y+  L+DED ++I  G FERHWG+F  DGQ KY L+ G     L+ A++V YL+ K
Sbjct: 310 AMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLGS-GNGLIPAKDVHYLAKK 369

Query: 372 WCVLNNNKDLSN--ASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQR 431
           WC+L  N +L +         AC  ADCT+L  G+SC N+    N+SYAFNSYYQ ++Q 
Sbjct: 370 WCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQL 429

Query: 432 PESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVFWLSQI 486
             +C F GL++++T DPS  +C+F + I++ ++   +AS ++     +AV  L  I
Sbjct: 430 DSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDAS--EASAMMPITRSTAVLLLLSI 478

BLAST of Csa6G181540 vs. Swiss-Prot
Match: E138_ARATH (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana GN=At1g64760 PE=1 SV=2)

HSP 1 Score: 369.0 bits (946), Expect = 8.0e-101
Identity = 204/473 (43.13%), Postives = 287/473 (60.68%), Query Frame = 1

Query: 1   MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKL 60
           M   L+L++ F  +  +L  L    V+ LG NWGT A+H L P  VVQ+L  NNI K+KL
Sbjct: 1   MSNLLALVVGFVIVIGHLGIL----VNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKL 60

Query: 61  LDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY 120
            D +   + AL+ S L++ +AIPN  LK++ S  +A + WV  NVTRY     GGV I +
Sbjct: 61  FDADETTMGALAGSGLEVMVAIPNDQLKVMTSYDRAKD-WVRKNVTRY--NFDGGVNITF 120

Query: 121 IAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESG--LP 180
           +AVG+EPFL SY   +      A+ANIQ A+ +  L + +K  VP + D + S +   +P
Sbjct: 121 VAVGNEPFLKSYNGSFINLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVP 180

Query: 181 SKGHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDS 240
           S G FRP++   M Q++ FL  + +P   +I PFLS   N +  L++A F + A P ND+
Sbjct: 181 SAGRFRPDIIGQMTQIVDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFF-DGAEPINDN 240

Query: 241 HRTYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYL 300
              Y N F+ ++DTLV+SL  +G   + I+V +VGWPT+G  +AN+ +A  F  GLL  L
Sbjct: 241 GIDYTNVFDANFDTLVSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRL 300

Query: 301 HSRSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNF-GQ-RT 360
            +  G+PLRP    +E Y+  LLDED ++I+ GPFERHWG+F FDGQ K+ ++  GQ ++
Sbjct: 301 GTNKGTPLRPTY--IEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQS 360

Query: 361 KNLVNAQNVEYLSPKWCVLNNN-KDLSNASARALEACSVADCTALAPGASCSNIGWPGNI 420
           K L+ AQNV YL  KWC  N   KDL+  +A    AC+ +DCTAL  G+SC+ +   GN 
Sbjct: 361 KFLIGAQNVPYLPNKWCTFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNA 420

Query: 421 SYAFNSYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDAS 469
           SYAFN ++Q  +Q   +C FQGLA ITT + S   C FP+QI  S++ S   S
Sbjct: 421 SYAFNMFFQVKNQDESACYFQGLATITTQNISQGQCNFPIQIVASSASSFSCS 463

BLAST of Csa6G181540 vs. Swiss-Prot
Match: E133_ARATH (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana GN=At2g01630 PE=1 SV=2)

HSP 1 Score: 207.2 bits (526), Expect = 4.0e-52
Identity = 155/482 (32.16%), Postives = 240/482 (49.79%), Query Frame = 1

Query: 6   SLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNP 65
           +L+L F FLF + SAL  S  S +G N GT  ++  SP +VV LL S NI +++L D + 
Sbjct: 3   ALLLLFLFLFAS-SAL--SQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADR 62

Query: 66  LVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGD 125
            +L A + + +Q+ I++PN  L  ++ S   A +WV  NV  Y         I  IAVG 
Sbjct: 63  SMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYP----ATNITTIAVGS 122

Query: 126 EPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRP 185
           E  +L+        ++ A+  IQAA+   NL+ +IKV  P S    + +S  PS+  F  
Sbjct: 123 E--VLTSLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHS-STIILDSFPPSQAFFNK 182

Query: 186 ELNKTMIQLLTFLTAHRSPFFASISPFLSFLQ-NKNISLDFALF------KETARPHNDS 245
             +  ++ LL FL +  SP   ++ P+  ++Q N  I LD+ALF      KE    +   
Sbjct: 183 TWDPVIVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLL 242

Query: 246 HRTYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAE---TFMKGLL 305
           H  Y N F+   D    ++S + F+ + IVV + GWP+ G  + + +T E   T+   L+
Sbjct: 243 H--YTNVFDAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLI 302

Query: 306 DYLHSRSGSPLRPRRPPLETYILSLLDEDRRNISTGPF-ERHWGVFTFDGQAKYHLNFGQ 365
            ++ +++G+P  P    + TYI  L +ED R    GP  E++WG+F  +G   Y L    
Sbjct: 303 QHVINKTGTPKHP-GTAVTTYIYELYNEDTR---PGPVSEKNWGLFYTNGTPVYTLRLAG 362

Query: 366 RTKNLVNAQNVEYLSPKWCVLNNNKDLSNASARALEAC--SVADCTALAPGASCSNIGWP 425
               L N    +  +  +C+     D     A    AC     DC+AL  G SC     P
Sbjct: 363 AGAILAN----DTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYE---P 422

Query: 426 GNI----SYAFNSYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDAS 471
            ++    +YAFN+YYQ+  +   SCDF+G+A +TT DPS   C FP   +++ +   + S
Sbjct: 423 DDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVFPGSAKSNQTLGNNTS 461

BLAST of Csa6G181540 vs. TrEMBL
Match: A0A0D2U7U1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G270800 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 3.8e-198
Identity = 333/467 (71.31%), Postives = 391/467 (83.73%), Query Frame = 1

Query: 10  FFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQ 69
           F  FLFL +  L  S   A+G NWGT +SHPL P KVV+LL SN ++K+KL D +PLVLQ
Sbjct: 4   FPVFLFLTILCLLGSTADAIGVNWGTMSSHPLPPPKVVELLKSNEVSKVKLFDADPLVLQ 63

Query: 70  ALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFL 129
           ALS S + +T+ IPN MLK LNSSRKAAESWVHDNVTRY S G  GVRIEY+AVGDEPFL
Sbjct: 64  ALSGSNIVVTLGIPNSMLKTLNSSRKAAESWVHDNVTRYFSHGEAGVRIEYVAVGDEPFL 123

Query: 130 LSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNK 189
            SYGD+++P+V+GA  NIQAA+TK NL   +KVVVPCS+D F+SESG PSKGHFR +LNK
Sbjct: 124 QSYGDQFHPFVIGAAMNIQAALTKANLAGEVKVVVPCSFDTFVSESGRPSKGHFRTDLNK 183

Query: 190 TMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELS 249
           TMI+LLTFL+ H SPFF +ISPF+SF QNKNISLDF+LFKE AR HNDSHRTYKNSF+LS
Sbjct: 184 TMIELLTFLSKHHSPFFVTISPFISFQQNKNISLDFSLFKENARHHNDSHRTYKNSFDLS 243

Query: 250 YDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPR 309
           YDTLV++LSKIGF  +DIVV ++GWPTDGA NA SS AETFMKGL+D+LHS++G+PLRPR
Sbjct: 244 YDTLVSALSKIGFPEIDIVVARIGWPTDGAANATSSIAETFMKGLIDHLHSKTGTPLRPR 303

Query: 310 RPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYLS 369
            PP+ET++ SLLDED R+I++G FERHWGVFTFDGQAKYH NFGQ TKNLVNAQ VEYL+
Sbjct: 304 NPPIETFMFSLLDEDHRSITSGNFERHWGVFTFDGQAKYHFNFGQGTKNLVNAQFVEYLA 363

Query: 370 PKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQR 429
           PKWCV+NNNKDLSNASA ALEACS +DCTAL+PG SCSNI WP NISYAFNSYYQQNDQR
Sbjct: 364 PKWCVVNNNKDLSNASASALEACSTSDCTALSPGGSCSNISWPSNISYAFNSYYQQNDQR 423

Query: 430 PESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPL 477
            +SCDF GL LITT+DPS + CRF V+++TS+S S   +++   I L
Sbjct: 424 ADSCDFGGLGLITTVDPSVDTCRFFVELQTSHSTSAHEAYIFLRIIL 470

BLAST of Csa6G181540 vs. TrEMBL
Match: A0A0B0NLN3_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_00171 PE=3 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 3.9e-195
Identity = 329/467 (70.45%), Postives = 388/467 (83.08%), Query Frame = 1

Query: 10  FFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQ 69
           F  FLFL +  L  S   A+G NWGT +SHPL P KVV+LL SN ++K+KL D +PLVLQ
Sbjct: 4   FPVFLFLTILCLLGSTADAIGVNWGTMSSHPLPPPKVVELLKSNEVSKVKLFDADPLVLQ 63

Query: 70  ALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFL 129
           ALS S + +T+ IPN MLK LNSSRKAAESWVHDNVTRY S G  GVRIEY+AVGDEPFL
Sbjct: 64  ALSGSNIVVTLGIPNSMLKTLNSSRKAAESWVHDNVTRYFSHGGAGVRIEYVAVGDEPFL 123

Query: 130 LSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNK 189
            SYGD+++P+V+GA  NIQA +TK NL   +KVVVPCS+D F+SESG PSKGHFR +LNK
Sbjct: 124 QSYGDQFHPFVIGAAMNIQATLTKANLAGEVKVVVPCSFDTFVSESGRPSKGHFRTDLNK 183

Query: 190 TMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELS 249
           TMI+LLTFL+ H S FF +ISPF+SF QNKNISLDF+LFKE A  HNDS RTYKNSF+LS
Sbjct: 184 TMIELLTFLSKHHSSFFVTISPFISFQQNKNISLDFSLFKENAHHHNDSLRTYKNSFDLS 243

Query: 250 YDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPR 309
           YDTLV++LSKIGF  +DIVV ++GWPTDGA NA SS AETFMKGL+D+LHS++G+PLRP+
Sbjct: 244 YDTLVSALSKIGFPEIDIVVARIGWPTDGAANATSSIAETFMKGLIDHLHSKTGTPLRPQ 303

Query: 310 RPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYLS 369
            PP+ET+I SLLDED R+I++G FERHWGVFTFDGQAKYH NFGQ TKNLVN+Q VEYL+
Sbjct: 304 NPPIETFIFSLLDEDHRSITSGNFERHWGVFTFDGQAKYHFNFGQGTKNLVNSQFVEYLA 363

Query: 370 PKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQR 429
           PKWCV+NNNKDLSNASA ALEACS +DCTAL+PG SCSNI WPGNISYAFNSYYQQNDQR
Sbjct: 364 PKWCVVNNNKDLSNASASALEACSTSDCTALSPGGSCSNISWPGNISYAFNSYYQQNDQR 423

Query: 430 PESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPL 477
            +SCDF GL LITT+DPS + CRF V+++TS+S S   +++   I L
Sbjct: 424 ADSCDFGGLGLITTVDPSVDTCRFFVELQTSHSTSAHEAYIFPRIIL 470

BLAST of Csa6G181540 vs. TrEMBL
Match: A0A061GYN7_THECC (O-Glycosyl hydrolases family 17 protein OS=Theobroma cacao GN=TCM_042208 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 5.6e-194
Identity = 328/476 (68.91%), Postives = 393/476 (82.56%), Query Frame = 1

Query: 1   MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKL 60
           +P  L LIL    L L    L  S   A+G NWG  +SHPL   KVV+LL SNN++K+KL
Sbjct: 2   LPQNLFLILL---LVLTFPCLLGSTAGAIGVNWGAMSSHPLPAPKVVELLKSNNVSKVKL 61

Query: 61  LDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY 120
            D +PLVLQALS + + +T  IPN MLK LNSSRKAAESWVHDNVTRY S G  GVRIEY
Sbjct: 62  FDADPLVLQALSGTNIAVTAGIPNSMLKALNSSRKAAESWVHDNVTRYFSHGRAGVRIEY 121

Query: 121 IAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK 180
           +AVGDEPFL SYG++++P+V+GA  NIQAA+TK NL S +KVVVPCS+D F++ES LPSK
Sbjct: 122 VAVGDEPFLQSYGEQFHPFVIGAAMNIQAALTKANLGSEVKVVVPCSFDTFVAESSLPSK 181

Query: 181 GHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHR 240
           GHFR +LNKTMI+LLTFL+   SPFF +ISPF+SF QNKNISLDF+LFKE  R HNDSHR
Sbjct: 182 GHFRTDLNKTMIELLTFLSKRHSPFFVTISPFISFQQNKNISLDFSLFKENVRTHNDSHR 241

Query: 241 TYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHS 300
           TYKNSF+LSYDTLV +LSKIGF  +DIVV ++GWPTDGA NAN+S AETFMKGL+D+LHS
Sbjct: 242 TYKNSFDLSYDTLVTALSKIGFPEMDIVVARIGWPTDGAANANTSVAETFMKGLVDHLHS 301

Query: 301 RSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLV 360
           ++G+PLRPR PP+ET+I SLLDED+R+I++G FERHWGVFTFDGQAKYH+NFGQ +KNLV
Sbjct: 302 KTGTPLRPRNPPIETFIFSLLDEDQRSITSGNFERHWGVFTFDGQAKYHVNFGQGSKNLV 361

Query: 361 NAQNVEYLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFN 420
           NAQ VEYL  KWCV+NNNKDLSNA+A ALEACSV+DCT L+PG SCSNI WPGNISYAFN
Sbjct: 362 NAQFVEYLPSKWCVVNNNKDLSNATASALEACSVSDCTVLSPGGSCSNISWPGNISYAFN 421

Query: 421 SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPL 477
           SYYQQ+DQR +SCDF GL L+TT+DPS  NCRF V+++TS+S S+  +++ + I L
Sbjct: 422 SYYQQHDQRADSCDFGGLGLVTTVDPSVGNCRFFVELQTSHSTSIHEAYVFQRIIL 474

BLAST of Csa6G181540 vs. TrEMBL
Match: M5WI71_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppb004066mg PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 2.4e-189
Identity = 322/476 (67.65%), Postives = 392/476 (82.35%), Query Frame = 1

Query: 1   MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKL 60
           MPP L  ++ F      L+ + +    A+G NWGT+ASHPL P KVV+LL SNN+TK+KL
Sbjct: 1   MPPNLCTVVVFL-----LATMAYGG-GAVGVNWGTTASHPLPPTKVVELLKSNNVTKVKL 60

Query: 61  LDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY 120
           LD +P VL+ALS S L +T+ IPN  L+ LNSS+KAAESWVHDNVTRY+S G  GV+IEY
Sbjct: 61  LDADPGVLEALSGSNLGVTVGIPNASLRTLNSSKKAAESWVHDNVTRYVSNGGSGVKIEY 120

Query: 121 IAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK 180
           +AVGDEPFL SYG++++P+V+GA  NI  A+ +  LES++KVVVPCS+D+FLSESG PSK
Sbjct: 121 VAVGDEPFLQSYGEQFHPFVIGAAMNIHIALARAKLESKVKVVVPCSFDSFLSESGHPSK 180

Query: 181 GHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHR 240
           GHFR +LN+TMI+LLTFL+ H SPFFA+ISPFLS  QNKNISLDF LFK  A+PHNDS R
Sbjct: 181 GHFRADLNRTMIELLTFLSKHSSPFFATISPFLSLHQNKNISLDFTLFKANAKPHNDSRR 240

Query: 241 TYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHS 300
           TYKNSF+LSYD LV +LS +GF  ++IVV Q+GWPTDGA NA SSTAETFMKGL+++LHS
Sbjct: 241 TYKNSFDLSYDILVTALSTVGFPKMEIVVSQIGWPTDGAANATSSTAETFMKGLVEHLHS 300

Query: 301 RSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLV 360
           +SG+PLRPR PP ETYI SLLDED+R++STG FERHWGVFTFDGQAKYH +F Q +KNLV
Sbjct: 301 KSGTPLRPRDPPSETYIFSLLDEDQRSVSTGNFERHWGVFTFDGQAKYHFDFIQGSKNLV 360

Query: 361 NAQNVEYLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFN 420
           +AQNVEYL  KWCV+NNNKDLSNA+A +LEACS+ADCTAL+PG SCSNI WPGNISYAFN
Sbjct: 361 DAQNVEYLPSKWCVVNNNKDLSNATASSLEACSLADCTALSPGGSCSNISWPGNISYAFN 420

Query: 421 SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPL 477
           SYYQQ++Q  +SC+F GL LITT+DPS +NCRF VQ+RTS S  L  ++L + + L
Sbjct: 421 SYYQQHNQSADSCNFGGLGLITTVDPSVDNCRFSVQLRTSLSDFLYPAYLTQWMTL 470

BLAST of Csa6G181540 vs. TrEMBL
Match: B9HNA2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s08030g PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 5.4e-189
Identity = 320/466 (68.67%), Postives = 383/466 (82.19%), Query Frame = 1

Query: 7   LILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPL 66
           L L  F LFL  S +T   V A+G NWGT++SHPL P KVV+LL SN ITK+KL D +PL
Sbjct: 3   LNLSLFLLFLTASNIT---VQAIGVNWGTTSSHPLPPDKVVELLKSNKITKVKLFDADPL 62

Query: 67  VLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDE 126
           VLQALS S + +T+ IPN MLK LNSS+K AESWVHDNVTRY+S G  GVRIEY+AVGDE
Sbjct: 63  VLQALSGSNIGVTVGIPNSMLKSLNSSKKVAESWVHDNVTRYVSSGNSGVRIEYVAVGDE 122

Query: 127 PFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPE 186
           PF  SYG++Y+P+V+GA  NIQ A+ +V+L +++KVVVPCSYD F SES LPSKGHFRP+
Sbjct: 123 PFQQSYGEQYHPFVIGAAMNIQTALARVSLANQVKVVVPCSYDTFQSESSLPSKGHFRPD 182

Query: 187 LNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSF 246
           LNKTM +LLTFLT H SPFFA+ISPF+   +NKNISLDF+LFKET    ND HRTYKNSF
Sbjct: 183 LNKTMTELLTFLTKHHSPFFATISPFIISHRNKNISLDFSLFKETKHSRNDGHRTYKNSF 242

Query: 247 ELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPL 306
           +L YDTLV++LS  GF  +D+VV ++GWPTDGA NA  S AETFMKGL+D+LHS+SG+PL
Sbjct: 243 DLGYDTLVSALSTAGFPEMDVVVAKIGWPTDGAANATPSAAETFMKGLMDHLHSKSGTPL 302

Query: 307 RPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVE 366
           RPR PP+ETYI SLLDED+R+I  G FERHWGVFTFDGQAKY+++ GQ +KNLVNAQ VE
Sbjct: 303 RPRNPPIETYIFSLLDEDQRSIVNGNFERHWGVFTFDGQAKYNVDLGQGSKNLVNAQYVE 362

Query: 367 YLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQN 426
           YLS KWCV+NNNKDLSNA+A AL+ACS ADC+AL+PG SC NI WP NISYAFN+YYQ +
Sbjct: 363 YLSSKWCVVNNNKDLSNATASALDACSTADCSALSPGGSCFNISWPANISYAFNNYYQVH 422

Query: 427 DQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLK 473
           DQR +SCDF GL LITT+DPS  NCRFPV++RTS+S+SL  + LL+
Sbjct: 423 DQRADSCDFGGLGLITTVDPSVGNCRFPVELRTSHSESLYGTCLLQ 465

BLAST of Csa6G181540 vs. TAIR10
Match: AT5G58480.1 (AT5G58480.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 617.1 bits (1590), Expect = 9.5e-177
Identity = 310/475 (65.26%), Postives = 370/475 (77.89%), Query Frame = 1

Query: 12  FFLFLNLSA---LTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVL 71
           F L L ++A   LT + V A+G NWGT ASHPL P KVV+LL SN I K+KL D +P VL
Sbjct: 6   FLLLLAVTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVL 65

Query: 72  QALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPF 131
           +ALS S + +TI I N MLK LN+S K AESWVHDNVTRY +GG   VRIEY+AVG+EPF
Sbjct: 66  RALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRYFNGGNR-VRIEYVAVGEEPF 125

Query: 132 LLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELN 191
           L SYG++Y P+V+GA  NIQ A+ K NL + +KVVVP S+D+FLSESG PS GHFR +LN
Sbjct: 126 LQSYGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLN 185

Query: 192 KTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFEL 251
           KTMI+LL+FLT H SPFF +ISPFLSF QNKNISLDF+LFKETA+ H D  +TY+NSF+L
Sbjct: 186 KTMIELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDL 245

Query: 252 SYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRP 311
           SYDTLV++L  IGFS VDIVV ++GWPTDGA+NA S TAE F KGL+ +L  ++ S    
Sbjct: 246 SYDTLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTAS---L 305

Query: 312 RRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYL 371
            RPP+ETYI SLLDED+RN+S G FERHWGVFTFDGQAKY+ +F    KN VNAQNV+YL
Sbjct: 306 PRPPVETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNH--KNQVNAQNVQYL 365

Query: 372 SPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQ 431
            PKWCV+NNNKDLSNASARALEAC+VADCT++ PG SCS I WPGN+SYAFNS YQQND 
Sbjct: 366 PPKWCVVNNNKDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDH 425

Query: 432 RPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVFWLS 484
             ESC+F GL LITT+DPS +NCRF +Q+ TS+S S   +F  ++ PL  +F LS
Sbjct: 426 SAESCNFGGLGLITTVDPSEDNCRFSIQLDTSHSSSQTPNF-FQSWPLLLLFLLS 473

BLAST of Csa6G181540 vs. TAIR10
Match: AT4G17180.1 (AT4G17180.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 413.7 bits (1062), Expect = 1.6e-115
Identity = 208/469 (44.35%), Postives = 296/469 (63.11%), Query Frame = 1

Query: 16  LNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQALSAST 75
           L+L  ++    SA+G NWGT + H + P  VV LL +N ITK+KL D NP  L+AL  + 
Sbjct: 12  LSLLLVSHEVESAIGVNWGTLSFHKMRPSTVVDLLKANKITKVKLFDANPDALRALMGTG 71

Query: 76  LQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFLLSYGDE 135
           +Q+ I IPN ML   NS     + +V  N++R++  G  G  I Y+AVG+EPFL  YG +
Sbjct: 72  IQVMIGIPNEMLSTFNS-----DLFVQQNLSRFI--GKNGADIRYVAVGNEPFLTGYGGQ 131

Query: 136 YYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNKTMIQLL 195
           +  YV+  + N+Q ++ + NL S +K+VVPC+ DA+  +S +PS+G FRPEL + M QL+
Sbjct: 132 FQNYVVPTMVNLQQSLVRANLASYVKLVVPCNADAY--QSNVPSQGMFRPELTQIMTQLV 191

Query: 196 TFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELSYDTLVA 255
           +FL ++ SPF  +I PFLS   N +   D+A F+ ++ P  D   TY N+F+ ++DTLVA
Sbjct: 192 SFLNSNGSPFVVNIYPFLSLYGNSDFPQDYAFFEGSSHPVPDGPNTYYNAFDGNFDTLVA 251

Query: 256 SLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPRRPPLET 315
           +L+K+G+  + IV+ ++GWPTDGA  AN + A  F +GL+ ++ S  G+PLRP  PP + 
Sbjct: 252 ALTKLGYGQMPIVIGEIGWPTDGAVGANLTAARVFNQGLISHVLSNKGTPLRPGSPPADV 311

Query: 316 YILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYLSPKWCVL 375
           Y+  LLDE  ++   G FERHWG+F+FDGQAKY LN G   + L NA+NV+YL  +WCV 
Sbjct: 312 YLFGLLDEGAKSTLPGNFERHWGIFSFDGQAKYRLNLGLGNRGLKNAKNVQYLPSRWCVA 371

Query: 376 NNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQRPESCDF 435
           + ++D++        ACS ADCT L  G SCS +G   NISYAFNSYYQ   Q  +SCDF
Sbjct: 372 HPSRDMTQVGDHLRLACSEADCTTLNDGGSCSQLGEKDNISYAFNSYYQMQMQHEKSCDF 431

Query: 436 QGLALITTIDPSNNNCRFPV---QIRTSNSQSLDASFLLKAIPLSAVFW 482
            GL ++T +DPS  +CRF V    I  S+S    A + +  I +  + W
Sbjct: 432 DGLGMVTFLDPSVGDCRFLVGVTDIGLSSSTEPMARWSIYHICIGLLIW 471

BLAST of Csa6G181540 vs. TAIR10
Match: AT5G58090.1 (AT5G58090.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 375.2 bits (962), Expect = 6.3e-104
Identity = 194/447 (43.40%), Postives = 265/447 (59.28%), Query Frame = 1

Query: 14  LFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQALSA 73
           L L   AL     S++G NWGT ASHPL P  VV++L  N I K+KL D     L+AL  
Sbjct: 7   LLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRALGK 66

Query: 74  STLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFLLSYG 133
           S +++ + IPN ML  L SS KAAE WV  NV+ ++S  T  V I Y+AVG+EPFL +Y 
Sbjct: 67  SGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHIS--TDNVNIRYVAVGNEPFLSTYN 126

Query: 134 DEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNKTMIQ 193
             Y      A+ NIQ AI K  L++++KV  P + D + S +  PS G FR  +   MI 
Sbjct: 127 GSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANIRDLMIT 186

Query: 194 LLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELSYDTL 253
           ++ FL+ +  PF  +I P++S   N +  +D+A F   A+P ND    Y N F+ +YDTL
Sbjct: 187 IVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYDTL 246

Query: 254 VASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPRRPPL 313
           V +L K GF  + I++ ++GWPTDG  NAN   A+ F +G + ++    G+P RP   P+
Sbjct: 247 VHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRP--GPI 306

Query: 314 ETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFG-QRTKNLVNAQNVEYLSPKW 373
           + Y+ SL+DED +++  G FERHWG+FTFDG  KY LN G   T  L+ A+ V YL  KW
Sbjct: 307 DAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGALIQAKGVRYLERKW 366

Query: 374 CVLNNNKDLSN--ASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQRP 433
           CV+  N  L +   +     ACS+ DCT+L  G SC+N+    NISYAFNSYYQ  DQ  
Sbjct: 367 CVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLD 426

Query: 434 ESCDFQGLALITTIDPSNNNCRFPVQI 458
            +C F  ++ +T  DPS   CRFP+ I
Sbjct: 427 TACKFPNISEVTKTDPSTGTCRFPIMI 449

BLAST of Csa6G181540 vs. TAIR10
Match: AT4G31140.1 (AT4G31140.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 375.2 bits (962), Expect = 6.3e-104
Identity = 195/476 (40.97%), Postives = 287/476 (60.29%), Query Frame = 1

Query: 12  FFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQAL 71
           FF+   L A     V  +G NWG+ A HPL P  VV+LL  N I K+KL + +  +L+AL
Sbjct: 10  FFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKAL 69

Query: 72  SASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFLLS 131
           S + +Q+ + IPN +L  L  S  AAE WV  NV+ ++S  + GV I Y+AVG+EPFL +
Sbjct: 70  SRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVS--SNGVDIRYVAVGNEPFLKA 129

Query: 132 YGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNKTM 191
           +   +    + A+ NIQ+AI K  L +++KV VP + D + S S LPS G FRPE+   M
Sbjct: 130 FNGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLM 189

Query: 192 IQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELSYD 251
           + ++ FL+ + +PF  +I PF+S   + N  ++FA F  T  P ND+ R Y N  + +YD
Sbjct: 190 LNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYD 249

Query: 252 TLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPRRP 311
           TLV SL K GF  + I+V +VGWPTDG  NAN   A  + +G ++   +  G+P+RP   
Sbjct: 250 TLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRP--G 309

Query: 312 PLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYLSPK 371
            ++ Y+  L+DED ++I  G FERHWG+F  DGQ KY L+ G     L+ A++V YL+ K
Sbjct: 310 AMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLGS-GNGLIPAKDVHYLAKK 369

Query: 372 WCVLNNNKDLSN--ASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQR 431
           WC+L  N +L +         AC  ADCT+L  G+SC N+    N+SYAFNSYYQ ++Q 
Sbjct: 370 WCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQL 429

Query: 432 PESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVFWLSQI 486
             +C F GL++++T DPS  +C+F + I++ ++   +AS ++     +AV  L  I
Sbjct: 430 DSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDAS--EASAMMPITRSTAVLLLLSI 478

BLAST of Csa6G181540 vs. TAIR10
Match: AT2G19440.1 (AT2G19440.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 371.3 bits (952), Expect = 9.1e-103
Identity = 198/451 (43.90%), Postives = 276/451 (61.20%), Query Frame = 1

Query: 26  VSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQALSASTLQLTIAIPNP 85
           V  LG NWGT A+H L P KVVQ+L  NNI K+KL D +   + ALS S L++ +AIPN 
Sbjct: 18  VDGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPND 77

Query: 86  MLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFLLSYGDEYYPYVMGAVA 145
            LK++ S  +A + WVH NVTRY     GGV I ++AVG+EPFL SY   +      A+ 
Sbjct: 78  QLKVMGSYDRAKD-WVHKNVTRY--NFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQ 137

Query: 146 NIQAAITKVNLESRIKVVVPCSYDAFLSESG--LPSKGHFRPELNKTMIQLLTFLTAHRS 205
           NIQ A+ +  L S +K  VP + D + S S   +PS G FRP++   M Q++ FL  + +
Sbjct: 138 NIQNALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSA 197

Query: 206 PFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELSYDTLVASLSKIGFS 265
           P   +I PFLS   N +  L++A F + A+P +D+   Y N F+ ++DTLV++L  +G  
Sbjct: 198 PITINIYPFLSLYGNDDFPLNYAFF-DGAKPVDDNGIAYTNVFDANFDTLVSALKAVGHG 257

Query: 266 TVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPRRPPLETYILSLLDE 325
            + I+V +VGWPT+G  +ANS +A  F  GLL  L    G+PLRP    +E Y+  LLDE
Sbjct: 258 DMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTY--IEVYLFGLLDE 317

Query: 326 DRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKN--LVNAQNVEYLSPKWCVLNNN-KD 385
           D ++I+ G FERHWG+F FDGQ K+ ++   + +N  L+ A+NV Y   KWC+ N   KD
Sbjct: 318 DAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKD 377

Query: 386 LSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQRPESCDFQGLAL 445
           L+  +A    AC+ +DCTAL  G+SC+ +   GN SYAFN Y+Q  +Q  ++C FQGLA 
Sbjct: 378 LTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLAT 437

Query: 446 ITTIDPSNNNCRFPVQIRTSNSQSLDASFLL 472
           ITT + S   C FP+QI  S + S  +S +L
Sbjct: 438 ITTKNISQGQCNFPIQIVASTASSFSSSLVL 462

BLAST of Csa6G181540 vs. NCBI nr
Match: gi|449450946|ref|XP_004143223.1| (PREDICTED: glucan endo-1,3-beta-glucosidase 9 [Cucumis sativus])

HSP 1 Score: 984.2 bits (2543), Expect = 8.4e-284
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 1

Query: 1   MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKL 60
           MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKL
Sbjct: 1   MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKL 60

Query: 61  LDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY 120
           LDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY
Sbjct: 61  LDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY 120

Query: 121 IAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK 180
           IAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK
Sbjct: 121 IAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK 180

Query: 181 GHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHR 240
           GHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHR
Sbjct: 181 GHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHR 240

Query: 241 TYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHS 300
           TYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHS
Sbjct: 241 TYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHS 300

Query: 301 RSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLV 360
           RSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLV
Sbjct: 301 RSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLV 360

Query: 361 NAQNVEYLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFN 420
           NAQNVEYLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFN
Sbjct: 361 NAQNVEYLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFN 420

Query: 421 SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVF 480
           SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVF
Sbjct: 421 SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVF 480

Query: 481 WLSQIVYLQ 490
           WLSQIVYLQ
Sbjct: 481 WLSQIVYLQ 489

BLAST of Csa6G181540 vs. NCBI nr
Match: gi|659127696|ref|XP_008463840.1| (PREDICTED: glucan endo-1,3-beta-glucosidase 9 [Cucumis melo])

HSP 1 Score: 934.9 bits (2415), Expect = 5.8e-269
Identity = 466/489 (95.30%), Postives = 473/489 (96.73%), Query Frame = 1

Query: 1   MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKL 60
           MPP LSLILFFFFL  NLSALTFS VSA+GFNWGT+ASHPLSPFKVVQLLNSNNITK+KL
Sbjct: 1   MPPNLSLILFFFFL--NLSALTFSTVSAVGFNWGTAASHPLSPFKVVQLLNSNNITKVKL 60

Query: 61  LDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY 120
           LDPNPLVLQALS ST+QLTI IPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY
Sbjct: 61  LDPNPLVLQALSGSTVQLTIGIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY 120

Query: 121 IAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK 180
           IAVGDEPFL SYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK
Sbjct: 121 IAVGDEPFLQSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK 180

Query: 181 GHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHR 240
           GHFRPELNKTMIQLLTFLT HRSPFFASISPFLSFLQNKNISLDFALFKETARPH+DS R
Sbjct: 181 GHFRPELNKTMIQLLTFLTTHRSPFFASISPFLSFLQNKNISLDFALFKETARPHDDSRR 240

Query: 241 TYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHS 300
           TYKNSFEL YDTLVASLSKIGF TVDIVV+QVGWPTDGA NANSSTAETFMKGLLDYLHS
Sbjct: 241 TYKNSFELIYDTLVASLSKIGFLTVDIVVDQVGWPTDGAGNANSSTAETFMKGLLDYLHS 300

Query: 301 RSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLV 360
           RSGSPLRPRRPPLETYILSLLDED+RNISTGPFERHWGVFTFDGQAKYHLNFGQ TKNLV
Sbjct: 301 RSGSPLRPRRPPLETYILSLLDEDQRNISTGPFERHWGVFTFDGQAKYHLNFGQGTKNLV 360

Query: 361 NAQNVEYLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFN 420
           NAQNVEYLSPKWCVLNNNKDLSNASA ALEACSVADCTALAPGASCSNIGWPGNISYAFN
Sbjct: 361 NAQNVEYLSPKWCVLNNNKDLSNASASALEACSVADCTALAPGASCSNIGWPGNISYAFN 420

Query: 421 SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPLSAVF 480
           SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAI LSAVF
Sbjct: 421 SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAISLSAVF 480

Query: 481 WLSQIVYLQ 490
           WLSQIVY Q
Sbjct: 481 WLSQIVYFQ 487

BLAST of Csa6G181540 vs. NCBI nr
Match: gi|823262333|ref|XP_012463914.1| (PREDICTED: glucan endo-1,3-beta-glucosidase 9 isoform X1 [Gossypium raimondii])

HSP 1 Score: 699.1 bits (1803), Expect = 5.4e-198
Identity = 333/467 (71.31%), Postives = 391/467 (83.73%), Query Frame = 1

Query: 10  FFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQ 69
           F  FLFL +  L  S   A+G NWGT +SHPL P KVV+LL SN ++K+KL D +PLVLQ
Sbjct: 4   FPVFLFLTILCLLGSTADAIGVNWGTMSSHPLPPPKVVELLKSNEVSKVKLFDADPLVLQ 63

Query: 70  ALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFL 129
           ALS S + +T+ IPN MLK LNSSRKAAESWVHDNVTRY S G  GVRIEY+AVGDEPFL
Sbjct: 64  ALSGSNIVVTLGIPNSMLKTLNSSRKAAESWVHDNVTRYFSHGEAGVRIEYVAVGDEPFL 123

Query: 130 LSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNK 189
            SYGD+++P+V+GA  NIQAA+TK NL   +KVVVPCS+D F+SESG PSKGHFR +LNK
Sbjct: 124 QSYGDQFHPFVIGAAMNIQAALTKANLAGEVKVVVPCSFDTFVSESGRPSKGHFRTDLNK 183

Query: 190 TMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELS 249
           TMI+LLTFL+ H SPFF +ISPF+SF QNKNISLDF+LFKE AR HNDSHRTYKNSF+LS
Sbjct: 184 TMIELLTFLSKHHSPFFVTISPFISFQQNKNISLDFSLFKENARHHNDSHRTYKNSFDLS 243

Query: 250 YDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPR 309
           YDTLV++LSKIGF  +DIVV ++GWPTDGA NA SS AETFMKGL+D+LHS++G+PLRPR
Sbjct: 244 YDTLVSALSKIGFPEIDIVVARIGWPTDGAANATSSIAETFMKGLIDHLHSKTGTPLRPR 303

Query: 310 RPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYLS 369
            PP+ET++ SLLDED R+I++G FERHWGVFTFDGQAKYH NFGQ TKNLVNAQ VEYL+
Sbjct: 304 NPPIETFMFSLLDEDHRSITSGNFERHWGVFTFDGQAKYHFNFGQGTKNLVNAQFVEYLA 363

Query: 370 PKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQR 429
           PKWCV+NNNKDLSNASA ALEACS +DCTAL+PG SCSNI WP NISYAFNSYYQQNDQR
Sbjct: 364 PKWCVVNNNKDLSNASASALEACSTSDCTALSPGGSCSNISWPSNISYAFNSYYQQNDQR 423

Query: 430 PESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPL 477
            +SCDF GL LITT+DPS + CRF V+++TS+S S   +++   I L
Sbjct: 424 ADSCDFGGLGLITTVDPSVDTCRFFVELQTSHSTSAHEAYIFLRIIL 470

BLAST of Csa6G181540 vs. NCBI nr
Match: gi|1009116170|ref|XP_015874629.1| (PREDICTED: glucan endo-1,3-beta-glucosidase 9 [Ziziphus jujuba])

HSP 1 Score: 694.9 bits (1792), Expect = 1.0e-196
Identity = 333/468 (71.15%), Postives = 391/468 (83.55%), Query Frame = 1

Query: 1   MPPTLSLILFFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKL 60
           MPP L LI+ FFF F  L+    S   A+G NWGT ASHPL P KVV+LL SN+I K+KL
Sbjct: 1   MPPNLRLIIIFFFFFFVLA----SRARAIGVNWGTMASHPLPPPKVVELLKSNDINKVKL 60

Query: 61  LDPNPLVLQALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEY 120
            D +PLVLQALS S L +TI IPN ML+ LNSS+KAAESWVHDNVTRY+S G  GVRIEY
Sbjct: 61  FDADPLVLQALSGSNLGVTIGIPNAMLRSLNSSKKAAESWVHDNVTRYISNGGSGVRIEY 120

Query: 121 IAVGDEPFLLSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSK 180
           +AVGDEPFL SYG+++ P+V+GA  NIQ A+ + NL+S++KVVVPCS+DAFLSES LPSK
Sbjct: 121 VAVGDEPFLQSYGEQFKPFVIGAAINIQTALVRANLDSKVKVVVPCSFDAFLSESNLPSK 180

Query: 181 GHFRPELNKTMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHR 240
           GHFR +LNKTMIQLLTFL  H SPFF +ISPFLS  QNKNISLDF LFKETA+ HNDSHR
Sbjct: 181 GHFRADLNKTMIQLLTFLNKHSSPFFVTISPFLSLQQNKNISLDFTLFKETAKAHNDSHR 240

Query: 241 TYKNSFELSYDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHS 300
           TYKNSF+LSYDTLVA+LS +GFS +DIVV Q+GWPTDGA NANSS AETF+KG + +LHS
Sbjct: 241 TYKNSFDLSYDTLVAALSTVGFSKMDIVVSQIGWPTDGAANANSSIAETFLKGFMKHLHS 300

Query: 301 RSGSPLRPRRPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLV 360
           + G+PLRP  PP+ETYI SLLDED+R+I+TG FERHWG+FTFDGQAKYH++FGQ  K+LV
Sbjct: 301 KMGTPLRPHNPPVETYIFSLLDEDQRSIATGNFERHWGLFTFDGQAKYHVDFGQGLKSLV 360

Query: 361 NAQNVEYLSPKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFN 420
           NAQNVEYLS KWCV+NNN DLSNA+A AL+AC+ ADCTAL+PG SCSNI WPGNISYAFN
Sbjct: 361 NAQNVEYLSSKWCVVNNNIDLSNATASALQACNNADCTALSPGGSCSNISWPGNISYAFN 420

Query: 421 SYYQQNDQRPESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDAS 469
           S+YQQ+DQ  +SCDF GL LITT+DPS   CRFPV++RTS+S  L ++
Sbjct: 421 SFYQQHDQSADSCDFGGLGLITTVDPSVGKCRFPVELRTSHSDCLHSN 464

BLAST of Csa6G181540 vs. NCBI nr
Match: gi|728836000|gb|KHG15443.1| (hypothetical protein F383_00171 [Gossypium arboreum])

HSP 1 Score: 689.1 bits (1777), Expect = 5.6e-195
Identity = 329/467 (70.45%), Postives = 388/467 (83.08%), Query Frame = 1

Query: 10  FFFFLFLNLSALTFSPVSALGFNWGTSASHPLSPFKVVQLLNSNNITKIKLLDPNPLVLQ 69
           F  FLFL +  L  S   A+G NWGT +SHPL P KVV+LL SN ++K+KL D +PLVLQ
Sbjct: 4   FPVFLFLTILCLLGSTADAIGVNWGTMSSHPLPPPKVVELLKSNEVSKVKLFDADPLVLQ 63

Query: 70  ALSASTLQLTIAIPNPMLKLLNSSRKAAESWVHDNVTRYLSGGTGGVRIEYIAVGDEPFL 129
           ALS S + +T+ IPN MLK LNSSRKAAESWVHDNVTRY S G  GVRIEY+AVGDEPFL
Sbjct: 64  ALSGSNIVVTLGIPNSMLKTLNSSRKAAESWVHDNVTRYFSHGGAGVRIEYVAVGDEPFL 123

Query: 130 LSYGDEYYPYVMGAVANIQAAITKVNLESRIKVVVPCSYDAFLSESGLPSKGHFRPELNK 189
            SYGD+++P+V+GA  NIQA +TK NL   +KVVVPCS+D F+SESG PSKGHFR +LNK
Sbjct: 124 QSYGDQFHPFVIGAAMNIQATLTKANLAGEVKVVVPCSFDTFVSESGRPSKGHFRTDLNK 183

Query: 190 TMIQLLTFLTAHRSPFFASISPFLSFLQNKNISLDFALFKETARPHNDSHRTYKNSFELS 249
           TMI+LLTFL+ H S FF +ISPF+SF QNKNISLDF+LFKE A  HNDS RTYKNSF+LS
Sbjct: 184 TMIELLTFLSKHHSSFFVTISPFISFQQNKNISLDFSLFKENAHHHNDSLRTYKNSFDLS 243

Query: 250 YDTLVASLSKIGFSTVDIVVEQVGWPTDGADNANSSTAETFMKGLLDYLHSRSGSPLRPR 309
           YDTLV++LSKIGF  +DIVV ++GWPTDGA NA SS AETFMKGL+D+LHS++G+PLRP+
Sbjct: 244 YDTLVSALSKIGFPEIDIVVARIGWPTDGAANATSSIAETFMKGLIDHLHSKTGTPLRPQ 303

Query: 310 RPPLETYILSLLDEDRRNISTGPFERHWGVFTFDGQAKYHLNFGQRTKNLVNAQNVEYLS 369
            PP+ET+I SLLDED R+I++G FERHWGVFTFDGQAKYH NFGQ TKNLVN+Q VEYL+
Sbjct: 304 NPPIETFIFSLLDEDHRSITSGNFERHWGVFTFDGQAKYHFNFGQGTKNLVNSQFVEYLA 363

Query: 370 PKWCVLNNNKDLSNASARALEACSVADCTALAPGASCSNIGWPGNISYAFNSYYQQNDQR 429
           PKWCV+NNNKDLSNASA ALEACS +DCTAL+PG SCSNI WPGNISYAFNSYYQQNDQR
Sbjct: 364 PKWCVVNNNKDLSNASASALEACSTSDCTALSPGGSCSNISWPGNISYAFNSYYQQNDQR 423

Query: 430 PESCDFQGLALITTIDPSNNNCRFPVQIRTSNSQSLDASFLLKAIPL 477
            +SCDF GL LITT+DPS + CRF V+++TS+S S   +++   I L
Sbjct: 424 ADSCDFGGLGLITTVDPSVDTCRFFVELQTSHSTSAHEAYIFPRIIL 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E139_ARATH1.7e-17565.26Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana GN=At5g58480 PE=1 SV=... [more]
E136_ARATH1.1e-10243.40Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana GN=At5g58090 PE=1 SV=... [more]
E135_ARATH1.1e-10240.97Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana GN=At4g31140 PE=1 SV=... [more]
E138_ARATH8.0e-10143.13Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana GN=At1g64760 PE=1 SV=... [more]
E133_ARATH4.0e-5232.16Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana GN=At2g01630 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0D2U7U1_GOSRA3.8e-19871.31Uncharacterized protein OS=Gossypium raimondii GN=B456_013G270800 PE=3 SV=1[more]
A0A0B0NLN3_GOSAR3.9e-19570.45Uncharacterized protein OS=Gossypium arboreum GN=F383_00171 PE=3 SV=1[more]
A0A061GYN7_THECC5.6e-19468.91O-Glycosyl hydrolases family 17 protein OS=Theobroma cacao GN=TCM_042208 PE=3 SV... [more]
M5WI71_PRUPE2.4e-18967.65Uncharacterized protein OS=Prunus persica GN=PRUPE_ppb004066mg PE=3 SV=1[more]
B9HNA2_POPTR5.4e-18968.67Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s08030g PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G58480.19.5e-17765.26 O-Glycosyl hydrolases family 17 protein[more]
AT4G17180.11.6e-11544.35 O-Glycosyl hydrolases family 17 protein[more]
AT5G58090.16.3e-10443.40 O-Glycosyl hydrolases family 17 protein[more]
AT4G31140.16.3e-10440.97 O-Glycosyl hydrolases family 17 protein[more]
AT2G19440.19.1e-10343.90 O-Glycosyl hydrolases family 17 protein[more]
Match NameE-valueIdentityDescription
gi|449450946|ref|XP_004143223.1|8.4e-284100.00PREDICTED: glucan endo-1,3-beta-glucosidase 9 [Cucumis sativus][more]
gi|659127696|ref|XP_008463840.1|5.8e-26995.30PREDICTED: glucan endo-1,3-beta-glucosidase 9 [Cucumis melo][more]
gi|823262333|ref|XP_012463914.1|5.4e-19871.31PREDICTED: glucan endo-1,3-beta-glucosidase 9 isoform X1 [Gossypium raimondii][more]
gi|1009116170|ref|XP_015874629.1|1.0e-19671.15PREDICTED: glucan endo-1,3-beta-glucosidase 9 [Ziziphus jujuba][more]
gi|728836000|gb|KHG15443.1|5.6e-19570.45hypothetical protein F383_00171 [Gossypium arboreum][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000490Glyco_hydro_17
IPR012946X8
IPR013781Glycoside hydrolase, catalytic domain
IPR017853Glycoside_hydrolase_SF
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008356 asymmetric cell division
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009736 cytokinin-activated signaling pathway
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0008270 zinc ion binding
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU114116cucumber EST collection version 3.0transcribed_cluster
CU130928cucumber EST collection version 3.0transcribed_cluster
CU146024cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G181540.1Csa6G181540.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU146024CU146024transcribed_cluster
CU114116CU114116transcribed_cluster
CU130928CU130928transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 29..352
score: 1.8
IPR012946X8 domainPFAMPF07983X8coord: 371..439
score: 1.5
IPR012946X8 domainSMARTSM00768X8_clscoord: 371..453
score: 8.0
IPR013781Glycoside hydrolase, catalytic domainGENE3DG3DSA:3.20.20.80coord: 29..352
score: 1.1
IPR017853Glycoside hydrolase superfamilyunknownSSF51445(Trans)glycosidasescoord: 29..352
score: 2.71
NoneNo IPR availablePANTHERPTHR32227FAMILY NOT NAMEDcoord: 24..441
score: 1.5E
NoneNo IPR availablePANTHERPTHR32227:SF128GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 9coord: 24..441
score: 1.5E

The following gene(s) are paralogous to this gene:

None