Csa6G020110 (gene) Cucumber (Chinese Long) v2

NameCsa6G020110
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionIST1-like protein; contains IPR005061 (Domain of unknown function DUF292, eukaryotic)
LocationChr6 : 2099046 .. 2103942 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTCTCCACCTCCTTTTCTTATTGATGCATAGATTACATTGATTAGGTTCAGTTTCATCATAATCTCCCCATTTTTCTCTAGATTTCAACTCGATCAAACGTAGAGCTTCTACACTTTTGGATTCTTCAAATTCAATATCTCCGTATCCTTTAATTCGGTCCTGAAAAGGATTTTCGTTTTGATCTTCATCTCCATGTCGATGCTTAATTCCTTCTTCAACAAGGGCTTCAAGGCTGCACAATGGTATCTCATCTTTTTATTTTGATTGCTTTTTCCCCATTTTCTCTCTTATTCTTCATTGAACTCTGTTGGTCACTGCTTTAATTTATTGATTTGAGATTTCAGAATTTTTGTTTTTGATAAAGGCGATCACCCTCTTCGGTTTATATGATTTACTTCACAATTTGCACTTTTGTATACAGTAAAACACTGCTTAAATTGACAATTCCACGAATAAAGCTGTTGAGGAATCGAAGAGAGATCCAGCTTAAGCAGATGCGCCGAGACATAGCTAAGCTTCTCGAAACAGGACAAGAAGCTACTGCTCGCATTCGTGTATGTGTTACTTCCTCAAGTGCATTAGATCATTTTGATGTTGAAAGAGGGAGGATAAGAAAGGAGGGGCATTGTTCGATGTCCTGGAACTTTTTGTTATCTTAGTTAAATCTTCTTTTGGATTCAGGTTGAACATATAATCAGAGAAGAGAATATGATGGCCGCTCAGGAAATTCTTGAATTGTTCTGTGAGCTTATTGTTGTTCGCCTTCCAATTATTGAAACACAAAGGTAATTTTTCCTAACCCATTTATTCTTCATTTGAAAATATTTCTTATTGTAATGTAATAAAACATGAGAAAGATCTGAATGCAACTGAGTTAAATAGGTTACACTTACAGTGCTTGAAGCAGATAGTAGATGCTCCCTACTATCTTTAATATACATGGACCTTTTTTTTGAGGGAAAAACATCCTTTAGTTTCATTGACAATGTGAAATTACAATAGTTGAAGATGTCCTATCGTTATGATTTACTAAACAGCTTTTTCAATGGTAAGTAACAAAACTTAAATCATATATATAGAAGTAGGGGTGCTTACAAGTTACAAAAACAATACAAGACACTTACATCATAATTATAGAAGTAAGGGTTGCTTACAAGTTACAAAAACAATACAAGAGTCAAGACTATAAGAGGGAACATGACAATAATGGTAAATGGTTCTTGTTCCACTTGTTCATGAACAATTTCCTGACTTCTTCCCTGAGGACAATGAAGCTCTTCATCTGGGGGTAATTTTAGATCACCAATCATGCACAGCTATGCAAGAAAGGGCAGAAATGCACGATCTACTGCACCAAACTAATGAATGATTTCAGTTGTTTATTATTTTATGGTGAACATGCAGTTGTTTCTTATCTACTGCACCAAACTAATGTGTCTTTCGAGTTGTTTGTTAGTTTGTGCTAGGCGTGCAACTGCCCATCATTTTGTGGGACGGTGTATGGCACTATTTAATAGGTTGGGATGTTGCTTATTAAGACAGGCTTCATTTTTCCTTGGTTAAGTCTTTTTGGAGAGACCACCCTCTTGAATGGTGGAAATGTACTGATTTGTACTAATCAATCTATCCTTTTCTAAGTGTTTTAGAGAGAGTAGCTTACAGGATACAATAACCAAAGTTTATTACTATTAATTGTATTTTTTCTCAAACATTTATTTATCTAAGATGTGCACGTATCTTATAAAACGTAGATTAGGGTGTTAGATCTCTAGTCGGTTTTGAGTTCAAATTTCGAACTCTCTACTGGACTCCTCTGTAAAGGCTTTATACTTTTTGATGAGCATTCAATTTATCTACATTAAATTCAAAGCTGCATTATGACACACAACTAACTAAATTTGTAACTATGGATTTGTCTACCAATTATACTTTCTTAAAGGCCGTTTTCTATGAGAATTTAATTTTATCAATAGATATGATATGTATATAATGAGTTCAGGATTTCCTTCATAATTTGTTAAGGAGCAGTTGCTTCATTTAAAAATGTGTACTTGATGACAAAAATTATAAATGTTGTATCTGCAGTTCTTTATTTCCCAATGTGATGTGAATTTATCTCAATTGTGTTTGTCCTTTTTCTATTGAAAATTGGCTTGTGGCCCTCTCATTCTTTGGGTAATTTCATGATGAGTAAGTATCCATATGTGTGCTATTTTGATTTGTAAAAATGCATATTAGAAATATGGTTGTATTTCACTTTGAAGTTTGAATACACATTTGCATGTAAAGTATTTTTGTGCATTTCATGTGGAATATTCTTTCATTTGAGTTGATTATTGCTGAACCTTCCTGCTATGATGGATGTTAAGTCTTAGATTGTTCAATGTTAACCTAGTTACCTAACCTGATCATTTAACTCCTTTGGATGACTGAAAACGCCATGCCTTGCAAACCGAAATTTAATTCATTGAGGGTGTTGCTAACTTGCAATAAAGTAACAAGTCTGCTTACCATTTCACAATTTTTCTGAGTTAACCACCAACCTCTTATTTTCTGGTGAAAATCCACGTGGTCTTTTGGATGATAGATTCTGCCTCAAGTTACCTGAAACTAGGAACTACATGTCCAAATTGCAATCTTAACTGCAAAATATTTCAAGTCTTACTTCATATTTACTGCAGAGAATTTATGCATTCCTTGTTGAATCAATGCTGATTAGTCGCAGCATCTCGATATTTCATTTTGTCTCATTTATTTCATCTAGTCCATTGTGATCTTTGTTTTTGTATTTCTAAATGGTGTATGTCTTTCTATGCTTATTTTGGAAGTATAAATGAACTTTTTTATTCTAACTTCACTTTGTTTCTTATGGTTATTTTCTTGAGACGTGCTCCTCCTACTTTATTCTCACCTCTAATGTCACTTGTCGCTGTAAAACCTCTAAATTTTCAGGGAATGTCCTCTAGACTTGAAAGAAGCGATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGTGCTGATCTTACAGAGCTTATCCAGGTTCAGATGCTTTTTGGTGCTAAATATGGAAAGGAATTTTTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGCGTTAATCGTCAGGTTCATAGAACTGCTTGTTTATGTTTGATTAGAAGGAAAGCTTTTCAGCAGTCCATAGGATTATATATTTCTTTTTAAAATTCGATTGCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTGTTGAAGGAAATTGCTGAAGAGCATGACTTAGATTGGAATCCTGCTGAAACTGAAGCTGAATTTAACAAATCTCCAGAAGATTTGCTTGTAAGTGTTGTTTCTTTTTGTCTCCACTTATAGTGACCCTTTTATGCCTTAAGCTTATCTTCTCCTTTTGCAGAATGGATCGACACAGTTTGTCGGCGCATCAAAATTACCTCTTCCTCAAGAGAAACACAATGAAACATCCAATACCACTACCGATTTGGCTAGTGGACCCCAACCTGACTCTGATTCTGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATGTCCGCAGCCTACTATAGATGCTGGCTCTGCACCATCAATGATCCCACCTCCATCTGTGTCGCCACCGCGTGAAACTGATCATGCTTCTTTTGAGTATTCTGGTATTCCTGAATCACCGCCACAAAATCTACACTCGAGACATGAGGAGGTGACTCTAGTGAGATCTGTTTCACCCAGCAACGATCAAATGAATGTTTCTGTTGGTGAAGATAAACAGTTTCTGCCTTTTATTACTCCCCCATCATTGTCACCTTCATTCTCTCGTAGACAAACTGAGTTGTCGCCATCCTCTGATCCAACGACGCCTGAGGAGAAGTTTGATATCAAACCTCGGATCAGAGAAGAGATAAATTCACCACTACCCTCTGTTTCAAGGGCTTCTGAGGAGAAGTATGGTTTCAAACCACGGTTCGAACCAGAGATAAATTCAACACCATCCTCTGTTTCGAGGACAATGAGTGAGGTCAATGCAGATCTCGGTGTTGATTTGCAGGACGTTCTGGCAGCTGCTCAGGCTGCTGCTGAAACCGCTGAACGTGCTGCCGCTGCAGCTCGTTCTGCTGCTAGTCTCGCCAAGGTTAGAATTGACGAGCTCACAAAGAAAAAGAATGATAAGGACCCTGAAATTAGTTGCGAAAATCCGTTTCACGGAGGTACTGCAAGTCCAGCAGATCATACAGAAACATACAAATTGAACCAACAAGATTCTCTGGGATACCATTCTAGTGCAATGCCATATTATTCACAAGATTCTTTTCAAGAACATCACAAATTAAGTCGCGATCCAGAAGTTTCTGATCACAGCCAACTCAAGCCAGAAAAATCAAGTTTCGATTACTCTCCTGGTAATTCACCATCTGAACATCAGACAGTAGCCCCTCATCAGCCACAGAGGCTCCCTTCAATGGACGACGACCCATACTTTTCATACCCAAATTTATTCACATCACAGAAACCAAATCCAGGATTCGATCATTCATCAGCTGGTGGTTCCTGATCTGCGTGAGGTTTGAGTTATATGCAAACGCGATGCCTTTGAGGCTCAAGGTTTCCTGAATTGATATTTTGTTCAGCTTTTATCATGAGTTTTCAATCTCTTTCAGAGCTATGGTAGCGAGCATTCAATGTACAAAACAACAGTAAAGATATAATGTACAATTGTTGATTTCTTTTCATGTACACTTTTTATGCCCTTTAATTTGTATTTGTTTGATCTCCAAACCATGTGGTATTTGCTTAATGAGAACGGAAGAGTTCTACGGTGGGGGCATTGAACACGAAAACTCAGAATTAGGATCC

mRNA sequence

ATGTCGATGCTTAATTCCTTCTTCAACAAGGGCTTCAAGGCTGCACAATGTAAAACACTGCTTAAATTGACAATTCCACGAATAAAGCTGTTGAGGAATCGAAGAGAGATCCAGCTTAAGCAGATGCGCCGAGACATAGCTAAGCTTCTCGAAACAGGACAAGAAGCTACTGCTCGCATTCGTGTTGAACATATAATCAGAGAAGAGAATATGATGGCCGCTCAGGAAATTCTTGAATTGTTCTGTGAGCTTATTGTTGTTCGCCTTCCAATTATTGAAACACAAAGGGAATGTCCTCTAGACTTGAAAGAAGCGATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGTGCTGATCTTACAGAGCTTATCCAGGTTCAGATGCTTTTTGGTGCTAAATATGGAAAGGAATTTTTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGCGTTAATCGTCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTGTTGAAGGAAATTGCTGAAGAGCATGACTTAGATTGGAATCCTGCTGAAACTGAAGCTGAATTTAACAAATCTCCAGAAGATTTGCTTAATGGATCGACACAGTTTGTCGGCGCATCAAAATTACCTCTTCCTCAAGAGAAACACAATGAAACATCCAATACCACTACCGATTTGGCTAGTGGACCCCAACCTGACTCTGATTCTGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATGTCCGCAGCCTACTATAGATGCTGGCTCTGCACCATCAATGATCCCACCTCCATCTGTGTCGCCACCGCGTGAAACTGATCATGCTTCTTTTGAGTATTCTGGTATTCCTGAATCACCGCCACAAAATCTACACTCGAGACATGAGGAGGTGACTCTAGTGAGATCTGTTTCACCCAGCAACGATCAAATGAATGTTTCTGTTGGTGAAGATAAACAGTTTCTGCCTTTTATTACTCCCCCATCATTGTCACCTTCATTCTCTCGTAGACAAACTGAGTTGTCGCCATCCTCTGATCCAACGACGCCTGAGGAGAAGTTTGATATCAAACCTCGGATCAGAGAAGAGATAAATTCACCACTACCCTCTGTTTCAAGGGCTTCTGAGGAGAAGTATGGTTTCAAACCACGGTTCGAACCAGAGATAAATTCAACACCATCCTCTGTTTCGAGGACAATGAGTGAGGTCAATGCAGATCTCGGTGTTGATTTGCAGGACGTTCTGGCAGCTGCTCAGGCTGCTGCTGAAACCGCTGAACGTGCTGCCGCTGCAGCTCGTTCTGCTGCTAGTCTCGCCAAGGTTAGAATTGACGAGCTCACAAAGAAAAAGAATGATAAGGACCCTGAAATTAGTTGCGAAAATCCGTTTCACGGAGGTACTGCAAGTCCAGCAGATCATACAGAAACATACAAATTGAACCAACAAGATTCTCTGGGATACCATTCTAGTGCAATGCCATATTATTCACAAGATTCTTTTCAAGAACATCACAAATTAAGTCGCGATCCAGAAGTTTCTGATCACAGCCAACTCAAGCCAGAAAAATCAAGTTTCGATTACTCTCCTGGTAATTCACCATCTGAACATCAGACAGTAGCCCCTCATCAGCCACAGAGGCTCCCTTCAATGGACGACGACCCATACTTTTCATACCCAAATTTATTCACATCACAGAAACCAAATCCAGGATTCGATCATTCATCAGCTGGTGGTTCCTGA

Coding sequence (CDS)

ATGTCGATGCTTAATTCCTTCTTCAACAAGGGCTTCAAGGCTGCACAATGTAAAACACTGCTTAAATTGACAATTCCACGAATAAAGCTGTTGAGGAATCGAAGAGAGATCCAGCTTAAGCAGATGCGCCGAGACATAGCTAAGCTTCTCGAAACAGGACAAGAAGCTACTGCTCGCATTCGTGTTGAACATATAATCAGAGAAGAGAATATGATGGCCGCTCAGGAAATTCTTGAATTGTTCTGTGAGCTTATTGTTGTTCGCCTTCCAATTATTGAAACACAAAGGGAATGTCCTCTAGACTTGAAAGAAGCGATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGTGCTGATCTTACAGAGCTTATCCAGGTTCAGATGCTTTTTGGTGCTAAATATGGAAAGGAATTTTTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGCGTTAATCGTCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTGTTGAAGGAAATTGCTGAAGAGCATGACTTAGATTGGAATCCTGCTGAAACTGAAGCTGAATTTAACAAATCTCCAGAAGATTTGCTTAATGGATCGACACAGTTTGTCGGCGCATCAAAATTACCTCTTCCTCAAGAGAAACACAATGAAACATCCAATACCACTACCGATTTGGCTAGTGGACCCCAACCTGACTCTGATTCTGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATGTCCGCAGCCTACTATAGATGCTGGCTCTGCACCATCAATGATCCCACCTCCATCTGTGTCGCCACCGCGTGAAACTGATCATGCTTCTTTTGAGTATTCTGGTATTCCTGAATCACCGCCACAAAATCTACACTCGAGACATGAGGAGGTGACTCTAGTGAGATCTGTTTCACCCAGCAACGATCAAATGAATGTTTCTGTTGGTGAAGATAAACAGTTTCTGCCTTTTATTACTCCCCCATCATTGTCACCTTCATTCTCTCGTAGACAAACTGAGTTGTCGCCATCCTCTGATCCAACGACGCCTGAGGAGAAGTTTGATATCAAACCTCGGATCAGAGAAGAGATAAATTCACCACTACCCTCTGTTTCAAGGGCTTCTGAGGAGAAGTATGGTTTCAAACCACGGTTCGAACCAGAGATAAATTCAACACCATCCTCTGTTTCGAGGACAATGAGTGAGGTCAATGCAGATCTCGGTGTTGATTTGCAGGACGTTCTGGCAGCTGCTCAGGCTGCTGCTGAAACCGCTGAACGTGCTGCCGCTGCAGCTCGTTCTGCTGCTAGTCTCGCCAAGGTTAGAATTGACGAGCTCACAAAGAAAAAGAATGATAAGGACCCTGAAATTAGTTGCGAAAATCCGTTTCACGGAGGTACTGCAAGTCCAGCAGATCATACAGAAACATACAAATTGAACCAACAAGATTCTCTGGGATACCATTCTAGTGCAATGCCATATTATTCACAAGATTCTTTTCAAGAACATCACAAATTAAGTCGCGATCCAGAAGTTTCTGATCACAGCCAACTCAAGCCAGAAAAATCAAGTTTCGATTACTCTCCTGGTAATTCACCATCTGAACATCAGACAGTAGCCCCTCATCAGCCACAGAGGCTCCCTTCAATGGACGACGACCCATACTTTTCATACCCAAATTTATTCACATCACAGAAACCAAATCCAGGATTCGATCATTCATCAGCTGGTGGTTCCTGA

Protein sequence

MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSELDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHSRHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEKFDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADHTETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS*
BLAST of Csa6G020110 vs. Swiss-Prot
Match: IST1_MOUSE (IST1 homolog OS=Mus musculus GN=Ist1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.4e-22
Identity = 67/178 (37.64%), Postives = 107/178 (60.11%), Query Frame = 1

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELIQVQMLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 GAKYGKEF--LSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNP 186
            AKY KE+  L    ++     VN +L+  LSV AP      + L EIA+ +++ + P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT---VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Csa6G020110 vs. Swiss-Prot
Match: IST1_HUMAN (IST1 homolog OS=Homo sapiens GN=IST1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.4e-22
Identity = 67/178 (37.64%), Postives = 107/178 (60.11%), Query Frame = 1

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELIQVQMLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 GAKYGKEF--LSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNP 186
            AKY KE+  L    ++     VN +L+  LSV AP      + L EIA+ +++ + P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT---VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Csa6G020110 vs. Swiss-Prot
Match: IST1_RAT (IST1 homolog OS=Rattus norvegicus GN=Ist1 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.4e-22
Identity = 67/178 (37.64%), Postives = 107/178 (60.11%), Query Frame = 1

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELIQVQMLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 GAKYGKEF--LSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNP 186
            AKY KE+  L    ++     VN +L+  LSV AP      + L EIA+ +++ + P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT---VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Csa6G020110 vs. Swiss-Prot
Match: IST1L_DICDI (IST1-like protein OS=Dictyostelium discoideum GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.3e-22
Identity = 76/258 (29.46%), Postives = 129/258 (50.00%), Query Frame = 1

Query: 8   FNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIR 67
           F   + + + K  LKL + RI++L+N++   ++  +R++A+LL    E +ARIRVE IIR
Sbjct: 4   FGPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIR 63

Query: 68  EENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELIQVQM 127
           +E ++   +I+E+ CEL+  R+ +I    E PL++KE+I ++ +++ R   + EL Q++ 
Sbjct: 64  DEYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQR-IQIPELEQIKN 123

Query: 128 LFGAKYGKEFLSAATELMPNC----GVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW 187
              AKYGK   + A     NC     VN +++  LS   P P    + L EIAE+ ++DW
Sbjct: 124 QLKAKYGKGLENEA-----NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 183

Query: 188 NPAETEAEFNKSPEDLLNGSTQFVGASKL--PLPQEKHNETSNTTTDLASGPQPDSDSEL 247
                 +++   P+ ++          ++  P PQ  H++           P P    + 
Sbjct: 184 ----CGSDYPPPPQLIMPQPIIVQQQPQILQPPPQIIHHQQQPQILQ----PPPQIIQQQ 243

Query: 248 DMLDFPEVPKMSVCPQPT 260
                P  P MS   QPT
Sbjct: 244 QQPQMPSFPIMSPPQQPT 247

BLAST of Csa6G020110 vs. Swiss-Prot
Match: IST1_BOVIN (IST1 homolog OS=Bos taurus GN=IST1 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 6.8e-22
Identity = 66/178 (37.08%), Postives = 106/178 (59.55%), Query Frame = 1

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           G KA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELIQVQMLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 GAKYGKEF--LSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNP 186
            AKY KE+  L    ++     VN +L+  LSV AP      + L EIA+ +++ + P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT---VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Csa6G020110 vs. TrEMBL
Match: A0A0A0KBZ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G020110 PE=4 SV=1)

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240
           LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE
Sbjct: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS 300
           LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS
Sbjct: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS 300

Query: 301 RHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEK 360
           RHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEK
Sbjct: 301 RHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEK 360

Query: 361 FDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQD 420
           FDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQD
Sbjct: 361 FDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQD 420

Query: 421 VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADH 480
           VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADH
Sbjct: 421 VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADH 480

Query: 481 TETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSP 540
           TETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSP
Sbjct: 481 TETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSP 540

Query: 541 SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 587
           SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS
Sbjct: 541 SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 586

BLAST of Csa6G020110 vs. TrEMBL
Match: A0A072U868_MEDTR (Regulator of Vps4 activity in the MVB pathway protein OS=Medicago truncatula GN=MTR_6g038150 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 9.2e-151
Identity = 332/599 (55.43%), Postives = 387/599 (64.61%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFF +GFKA++CKTLLKL+IPRIKLLRNRRE QLK MRR+IAKLLETGQEATARI
Sbjct: 1   MSMLNSFFAQGFKASKCKTLLKLSIPRIKLLRNRRENQLKNMRREIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCEL+ VRLPIIE+QRE PLDLKEAISS+CFAAPRCADL 
Sbjct: 61  RVEHIIREENMMAAQEILELFCELVSVRLPIIESQREIPLDLKEAISSICFAAPRCADLP 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELIQVQ  F  KYGKEF+SAATEL P+CGVNRQ+IELLSVRAPSPEKKL LLK+IA EH+
Sbjct: 121 ELIQVQQQFATKYGKEFISAATELRPDCGVNRQIIELLSVRAPSPEKKLTLLKDIAVEHE 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGP---QPDS 240
           LDW+PA  E EF K  EDLLNG T+F G SKLPL +EKH+E   +T D+   P   Q DS
Sbjct: 181 LDWDPATAETEFFKKHEDLLNGPTEFFGGSKLPLAEEKHDEELYSTHDVHDTPKKEQLDS 240

Query: 241 DSELDMLDFPEVPKMSVCPQ--PTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPP 300
           DS+ DMLDFPEVPK  V  Q  PT + G+AP  + PP   P  E D  S  +S       
Sbjct: 241 DSDSDMLDFPEVPKTYVQMQSLPTANFGTAPDTVTPPGGMPHPEVDLHSSSHS----EDF 300

Query: 301 QNLHSRHEEVTLVRSVSPSNDQMNVSVG--EDKQFLPFITPPSLSPSFSRRQTELSPSSD 360
            +L   H E T+ R      D+ + S G  E KQF PFI PPS SP+        SP+S 
Sbjct: 301 TDLKQEHVESTVHR------DESHTSFGKTESKQFSPFIAPPSESPASYNPGHSDSPTSF 360

Query: 361 PTTPEEKFDIKPRIR----EEINSPLPSVSRASEEKYGFKPRFE-------PEINSTPSS 420
           PT   EK    P +        +S LP  + A++ +    P  E        + + +P  
Sbjct: 361 PT---EKSISSPSLSSAPYSARHSDLPPFTSAAKSE---SPPSETVASYNATKHSDSPPK 420

Query: 421 VSRTMSEVNADLGVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTK-KKNDKD 480
           +S T SE N    VDL DV+AAA AAAETAERAAAAARSAASLA++RI ELTK + N+  
Sbjct: 421 LSTTKSESN----VDLNDVVAAAHAAAETAERAAAAARSAASLAQLRISELTKMRSNEHI 480

Query: 481 PEISCENPFHGGTASPADHTETYKLNQQDSLGYHSSAMPY---YSQDSFQEHHKLSRDPE 540
           P+ S ENPF+ G     + T T + + + ++G      PY   +      E H  S    
Sbjct: 481 PDSSSENPFYAG---GNNETSTPERDHKSAIG-----NPYANVFKDHDIHEDHNASIGSH 540

Query: 541 VSDHSQLKPEKSSFDYSPGNSPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPG 578
            S        K  FD SP            HQP+RLPSMDDD YFSYPNLF SQ  N G
Sbjct: 541 SSSFPSFDTLKEDFDSSPPTDRVLDDKSNSHQPKRLPSMDDDTYFSYPNLFNSQNSNVG 571

BLAST of Csa6G020110 vs. TrEMBL
Match: A0A059BY61_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F04150 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 2.5e-148
Identity = 328/595 (55.13%), Postives = 403/595 (67.73%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MS+++SFF KGFKAA+CKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSIMDSFFAKGFKAAKCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREE MMAAQE+LELFCELI VRLP+IE QRECP+DLKEAISSVCFAAPRCADL 
Sbjct: 61  RVEHIIREEKMMAAQEVLELFCELISVRLPVIEAQRECPIDLKEAISSVCFAAPRCADLP 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELIQVQMLF +KYGKEF++AA+ELMP+CGVNRQLIELLSVRAPSPE KLKLLKEIAEEH+
Sbjct: 121 ELIQVQMLFASKYGKEFVAAASELMPDCGVNRQLIELLSVRAPSPEAKLKLLKEIAEEHE 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240
           L+W+PA +E+E  KS EDLLNGSTQF   SKLPLP+EKH+++  +  D A   QPDSD+ 
Sbjct: 181 LEWDPAASESELFKSHEDLLNGSTQFGSGSKLPLPKEKHDDSLYSRPDHALDEQPDSDTG 240

Query: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPP-PSVSPPRE---TDHASFEYSGIPESPPQ 300
            + LDFPEVP++SV  +P   A SAP +IPP P  + P +    D  S + SG  E+   
Sbjct: 241 FESLDFPEVPQISV--RPNSSAVSAPEIIPPNPVAASPADHKVGDEIS-QLSGTVETLSL 300

Query: 301 NLHSRHEEVTLVRSVSPSND--QMNVSVGEDKQFLPFITPPSLSP-SFSRRQTELSPSSD 360
             H   +EV     +   ++  + +V V EDKQFLPFI PPSLS  SFS R++  +  S 
Sbjct: 301 QKHVDPKEVVEEHLIDHKDETAKSSVDVKEDKQFLPFIAPPSLSSVSFSVRESFPARESA 360

Query: 361 PTTPEEKFDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNAD 420
           P +   +                  ++++ +    +  F    +S P + + T       
Sbjct: 361 PPSSLRR------------------TKSNAQDVDSQDVFAAAQSSLPRTKNNTQD----- 420

Query: 421 LGVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGG 480
             VDLQDVLAAAQAAAE+AERAA+AARSAASLA+VRI ELT+KK+    +   +NPF+  
Sbjct: 421 --VDLQDVLAAAQAAAESAERAASAARSAASLAQVRITELTRKKSAVLDD--SDNPFYAA 480

Query: 481 TASPADHTETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFD 540
           T   + +  T +L  + SL   +S   + S D  Q  HK  ++  VS  +Q  PE+  FD
Sbjct: 481 TPE-STNAPTPELEHKRSL--ENSDQRFDSMDD-QVFHKDLKESGVS--NQPPPEEVKFD 540

Query: 541 YSPGNSPSE--HQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 587
                S  +        HQP+R  SM D+PYFSYPNLF+SQ  N G    S+  S
Sbjct: 541 SEASESSHKVHDYNSEYHQPERSTSM-DEPYFSYPNLFSSQSINVGSGSQSSDNS 558

BLAST of Csa6G020110 vs. TrEMBL
Match: A0A061FL82_THECC (Regulator of Vps4 activity in the MVB pathway protein OS=Theobroma cacao GN=TCM_036628 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 3.5e-142
Identity = 316/592 (53.38%), Postives = 376/592 (63.51%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MS+L+SFF+KGFKAA+CKTLLKLTIPRIKLLRNRREIQ+KQMRRDIAKLLETGQEATARI
Sbjct: 1   MSVLDSFFSKGFKAAKCKTLLKLTIPRIKLLRNRREIQIKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELI VRLPIIETQRECPLDLKEAISSVCFAAPRCADL 
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIAVRLPIIETQRECPLDLKEAISSVCFAAPRCADLP 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL+QVQMLF +KYGKEF+SAATEL P+CGVNRQLIELLSVRAPSPE KLKLLKEIAEEH+
Sbjct: 121 ELLQVQMLFASKYGKEFISAATELRPDCGVNRQLIELLSVRAPSPEVKLKLLKEIAEEHE 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240
           LDW+PA TE EF K  EDLLNG TQFV  SKLPLP EKH+ET N+ TD A   QPDSDS+
Sbjct: 181 LDWDPASTETEFFKPHEDLLNGPTQFVSGSKLPLPAEKHDETLNSATDHAQNEQPDSDSD 240

Query: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS 300
            + LDFP+VPK+S+  +P+ +A SAP++ PP + +P  E DH S  + G P         
Sbjct: 241 FEPLDFPDVPKVSL--RPSANAASAPAISPPSAAAPDPEIDHGSSRHFGAPGYVLPMSPL 300

Query: 301 RHEEVTLVRSVSPSNDQMNVSVG--EDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPE 360
            HE V    SV+  N+  N   G  E+KQFLPFI+PPS+S S S R     PS+ P+   
Sbjct: 301 EHETVMEESSVTKENEMPNDPAGAKENKQFLPFISPPSVSSSISAR-----PSNPPSAIS 360

Query: 361 EKFDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDL 420
                  +I+ E N  L  V  A++           E     ++ +R+ + +        
Sbjct: 361 -------KIKIEANVDLQDVLAAAQA--------AAETAERAAAAARSAASL-------- 420

Query: 421 QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPA 480
                A    AE  ++      S++S      D   +  + + P    +N F      PA
Sbjct: 421 -----AQVRIAELTQKKDQVTESSSSENPFHTDVPHQPPSTEKPNFDHQNSF----GDPA 480

Query: 481 DHTETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRD-PEVSDHSQLKPEKSSFDYSPG 540
           +                      YS DS   HH   R   EV++   L   K  FD SP 
Sbjct: 481 E--------------------VLYSPDS---HHDQERQGSEVANLPSLDKLKLGFD-SPV 521

Query: 541 NSPSEHQTVAPH---QPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 587
           +  S+H     H   QPQRLPSMDD+ YFSYPNLF+SQK + G     +GGS
Sbjct: 541 S--SDHALEGDHPQKQPQRLPSMDDE-YFSYPNLFSSQKSDLG-----SGGS 521

BLAST of Csa6G020110 vs. TrEMBL
Match: A0A164X3F8_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_019987 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.1e-140
Identity = 311/586 (53.07%), Postives = 379/586 (64.68%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML+SFFNKGFKA +CKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLDSFFNKGFKAPRCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREE MMAAQEI+ELFCELI VRLPIIE+QRECPLDLKEAISS+CFAAPRCADL 
Sbjct: 61  RVEHIIREEKMMAAQEIIELFCELISVRLPIIESQRECPLDLKEAISSICFAAPRCADLP 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELIQVQMLF  KYGKEF+ AATELMP CGVNRQLIELLSVRAP+P+ KLKLLK+IAEEH+
Sbjct: 121 ELIQVQMLFAGKYGKEFVVAATELMPECGVNRQLIELLSVRAPAPDVKLKLLKDIAEEHE 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNE-TSNTTTDLASGPQPDSDS 240
           LDW+P  +E+E  K  EDLLNG TQFV  +K+PLP+EK ++   +T ++       DSD+
Sbjct: 181 LDWDPTASESELLKPHEDLLNGPTQFVSETKVPLPEEKKDDDVDDTGSEQVPHYASDSDA 240

Query: 241 ELDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLH 300
             D  DFPEVP  S+  +P+   G+AP M+P P+ S     D  S   SG  ES   +L 
Sbjct: 241 GFDTSDFPEVPTASL--RPSKGTGTAPEMLPFPA-SALSGLDPESTNSSGDEESYSHDLK 300

Query: 301 SRHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEE 360
           +  EE+   +  S      +  V EDKQFLPFI+PP              PSS       
Sbjct: 301 AELEEMLRDKPESEDFKPPSTLVDEDKQFLPFISPPP-------------PSS------- 360

Query: 361 KFDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQ 420
                      ++ P PS S A E           EIN  PS V RT ++      VDL 
Sbjct: 361 -----------VSLPPPSSSTALER----------EINPPPS-VLRTSTD-----DVDLL 420

Query: 421 DVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPAD 480
           DVLAAAQAAA +AE AAAAARSAASLA+ RI+E+  K+N    +I+ +NPFH G +S +D
Sbjct: 421 DVLAAAQAAASSAEHAAAAARSAASLAEFRINEIVSKRNVGGSQITGDNPFHSG-SSVSD 480

Query: 481 HTETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNS 540
             +   L+ ++S      +   +S+    +H      PE+      + + S  + +P  S
Sbjct: 481 TGKKQALDHENSFTDSDISNSLHSRLHLGDH------PELETTKYPEYDNSELNSNPSIS 529

Query: 541 PSEH--QTVAPHQPQRLPSMDDDPYFSYPNLF----TSQKPNPGFD 580
            + +  Q   PHQP+R+ S++D+ Y SYPNLF     S    P FD
Sbjct: 541 ENLNLGQYTPPHQPKRVDSLEDESYLSYPNLFNRGPNSVSREPSFD 529

BLAST of Csa6G020110 vs. TAIR10
Match: AT1G34220.1 (AT1G34220.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 397.1 bits (1019), Expect = 1.9e-110
Identity = 275/663 (41.48%), Postives = 353/663 (53.24%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML+SFFNKGFKAA+CKTLLKLTIPRIKL+RNRRE Q+KQMRR+IAKLLETGQEATARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREE MMAAQEILELFCELI VRLPIIE QRECPLDLKEAISSVCFAAPRC+DLT
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNR---------------------------- 180
           EL QVQ+LF +KYGKEF++AA+EL P+ GVNR                            
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNS 180

Query: 181 --QLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNPAETEAEFNKSPEDLLNGSTQFVGAS 240
             QL+ELLSVRAPSPE KLKLLKEIAEEH+LDW+PA TE +  KS EDLL+G  QF G S
Sbjct: 181 ILQLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGS 240

Query: 241 KLPLPQEKHNETSNTTTDLASGPQPDSDSELDMLDFPEVPKMSVCPQPTIDAGSAPSMIP 300
           KLPLP+E+ NE +N T+  A+  + DSDSE D+LDFPEVP + + P P            
Sbjct: 241 KLPLPEEQ-NEKTNLTSLSAAKEKSDSDSEYDILDFPEVPNVLLRPTP-----------G 300

Query: 301 PPSVSPPRETDHASFEYSGIPESPPQNLHSRHEEVTLVRSVSPSNDQMNVSVGEDKQFLP 360
             SV+ P     AS+E++   + P  + ++  E+    R   P+     V  G+      
Sbjct: 301 ATSVNAPDAAKSASYEHTS-HDLPFDSENAGVEKTASKRDEHPAKASKTVVEGQQ----- 360

Query: 361 FITPPSLSPSFSRRQTELSPSSD---PTTPEEKFDIKPRIREEINSPLPSVSRASEEKYG 420
             + P L  SF ++     PS D   P   +E    +   R+  +  L  V  A++    
Sbjct: 361 --SSPILMESFEKKNYS-PPSIDAVGPIPTKESGASRDTPRKISDGDLQDVLMAAQAAAD 420

Query: 421 FKPRFEPEINSTPSSVSRTMSEVNADLGVDL-----QDVLAAAQAAAETAERAAAAARSA 480
              R      S  S     ++E+             ++   A        +   ++A S+
Sbjct: 421 SAERAASAARSAASLAQLRINELTRKTSDQYPESPSENPFHAPSMGNLQFDHQNSSASSS 480

Query: 481 ASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADHTETYKLNQQDSLGYHSSAMPYY 540
             L +++  E +   N +      +N     T S  +  +  + N   S     +   ++
Sbjct: 481 GDLTELQRAETSSLFNSE------QNNQQPQTHSSMEKPQFDRQNSSFSSYGDLTPQRFH 540

Query: 541 SQDSFQEHHKLSRDPEVSDHSQLKP------EKSSFDYSPGNSPSEHQTVAPH------- 577
           S +  Q  H+ S     S++  L P      EK  FD+   +S S +  + PH       
Sbjct: 541 SMEKPQFDHQNSSG---SNYDDLTPQRFSSLEKPQFDHQ-NSSGSNYDDLTPHRFPSMEK 600

BLAST of Csa6G020110 vs. TAIR10
Match: AT4G35730.1 (AT4G35730.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 259.2 bits (661), Expect = 6.0e-69
Identity = 160/396 (40.40%), Postives = 219/396 (55.30%), Query Frame = 1

Query: 6   SFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHI 65
           S F +GF +++CKT  K+ + RIKL+RN+R + +KQMRRDIA LL++GQ+ATARIRVEH+
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 66  IREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELIQV 125
           IRE+N+ AA EI+ELFCELIV RL II  Q++CP+DLKE I+S+ FAAPRC+++ EL  +
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 126 QMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNP 185
           + +F  KYGK+F+SAAT+L P+CGVNR LI+ LSVR P  E KLK++KEIA+E  +DW+ 
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDT 199

Query: 186 AETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDS------DS 245
            ETE E  K  E+ ++G  +FV AS LP+ +   NE  + T  +       S      D+
Sbjct: 200 TETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDT 259

Query: 246 ELDMLDFPEVPKMSVCPQ--PTIDAGSAPSMIPPPSVSPPRETDHASFEY-SGIPESPPQ 305
           E       E+ K +V      ++ A    S     SVS    T     +Y       P  
Sbjct: 260 ESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGS 319

Query: 306 NLHSRHEEVTLV--------RSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTEL 365
              SR  E +          R +   +   N  + E      +    + +    RR+   
Sbjct: 320 RRQSRDSETSSYYAKPGAENRGMGRRHSYNNPGINESDYEEEYTNTEAEAKETMRRRHSY 379

Query: 366 SPSS--DPTTPEEKFDIKPRIREEINSPLPSVSRAS 383
           +P S   P T E KFD      EE     PS  R S
Sbjct: 380 NPRSVPPPATSEIKFDESDYYEEETEPDEPSQGRVS 415

BLAST of Csa6G020110 vs. TAIR10
Match: AT1G25420.1 (AT1G25420.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-65
Identity = 118/205 (57.56%), Postives = 160/205 (78.05%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MS+LN  FN+G   A+CKT L L I R+KLL+N+R++QLK M+++IA  L+ GQE  ARI
Sbjct: 1   MSLLNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEH+IRE N+ AA EILELFCE I+ R+PI+E+++ECP +L+EAI+S+ FAAPRC+++ 
Sbjct: 61  RVEHVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVP 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           +L+Q++ LFG KYGKEF+  A+EL P+ GVNR +IE LS  +PS   +LK+LKEIA+E+ 
Sbjct: 121 DLLQIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYS 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQ 206
           L+W+ + TEAEF KS EDLL G+ Q
Sbjct: 181 LNWDSSATEAEFMKSHEDLLGGAKQ 205


HSP 2 Score: 36.2 bits (82), Expect = 8.3e-02
Identity = 22/55 (40.00%), Postives = 28/55 (50.91%), Query Frame = 1

Query: 394 PEINSTPSSVSRTMSEVNADLGVDLQDVLAAAQAAAETAERAAAAARSAASLAKV 449
           P   S PSS   +  +   D   +  DV+  A+AA  +A+RA AAAR+AA L  V
Sbjct: 250 PVSKSMPSSKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQLVNV 304

BLAST of Csa6G020110 vs. TAIR10
Match: AT2G19710.1 (AT2G19710.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 210.3 bits (534), Expect = 3.2e-54
Identity = 101/209 (48.33%), Postives = 151/209 (72.25%), Query Frame = 1

Query: 4   LNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVE 63
           +     +GFK A+CKT L++   R+K+L+N++EIQ+KQ+RR++A+LLE+GQ  TARIRVE
Sbjct: 1   MKKVLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVE 60

Query: 64  HIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELI 123
           H++REE  +AA E++ ++CEL+VVRL +IE+Q+ CP+DLKEA++SV FA+ R +D+ EL 
Sbjct: 61  HVVREEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELS 120

Query: 124 QVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW 183
           ++   F  KYGK+F ++A EL P+ GV+R L+E LS +AP    K+K+L  IAEEH++ W
Sbjct: 121 EIFKQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVW 180

Query: 184 NPAETEAEFNKSPEDLLNGSTQFVGASKL 213
             A++  E +    +LLNG+  F  AS +
Sbjct: 181 E-AQSFVESDPKDTELLNGANSFQPASSM 208


HSP 2 Score: 32.0 bits (71), Expect = 1.6e+00
Identity = 17/27 (62.96%), Postives = 19/27 (70.37%), Query Frame = 1

Query: 420 DVLAAAQAAAETAERAAAAARSAASLA 447
           D   AA+AAAE AERA+ AAR+AA L+
Sbjct: 309 DSTDAARAAAEAAERASFAARAAAELS 335

BLAST of Csa6G020110 vs. TAIR10
Match: AT4G29440.1 (AT4G29440.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 191.0 bits (484), Expect = 2.0e-48
Identity = 90/200 (45.00%), Postives = 140/200 (70.00%), Query Frame = 1

Query: 4   LNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVE 63
           +    ++ FK A+CK  L++   R+K+L+N+++ Q+KQ+RR++A LLE+GQ  TA+IRVE
Sbjct: 1   MKKVLHRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRVE 60

Query: 64  HIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELI 123
           H++REE  +AA E++ ++CEL+V RL +I++Q+ CP DLKEA++SV +A+ R  D+ EL 
Sbjct: 61  HVVREEKTVAAYELVGIYCELLVARLGVIDSQKTCPNDLKEAVASVLYASQRLTDVGELS 120

Query: 124 QVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW 183
            +   F AKYGK+F+SAA  L P+ GV+R L+E LSV+AP    K+K+L EIA +H++ W
Sbjct: 121 DIVKHFSAKYGKDFVSAAIGLQPDSGVSRLLVEKLSVKAPDGPTKIKILTEIATQHNVTW 180

Query: 184 NPAETEAEFNKSPEDLLNGS 204
              E E+     P++ ++ S
Sbjct: 181 ---EAESLVESDPKETMSAS 197


HSP 2 Score: 40.0 bits (92), Expect = 5.7e-03
Identity = 25/61 (40.98%), Postives = 33/61 (54.10%), Query Frame = 1

Query: 420 DVLAAAQAAAETAERAAAAARSAASLAK----VRIDELTKKKNDKDPEISCENPFHGGTA 477
           D  +AA+AAAE+AERA+ AAR AA L+     + +   T+ +N    E    NP H  T+
Sbjct: 297 DATSAARAAAESAERASFAARRAAELSSKERMMMMQNSTESRNSSSYENLRSNPPHSRTS 356

BLAST of Csa6G020110 vs. NCBI nr
Match: gi|449441181|ref|XP_004138362.1| (PREDICTED: uncharacterized protein LOC101208940 [Cucumis sativus])

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240
           LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE
Sbjct: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS 300
           LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS
Sbjct: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS 300

Query: 301 RHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEK 360
           RHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEK
Sbjct: 301 RHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEK 360

Query: 361 FDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQD 420
           FDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQD
Sbjct: 361 FDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQD 420

Query: 421 VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADH 480
           VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADH
Sbjct: 421 VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADH 480

Query: 481 TETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSP 540
           TETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSP
Sbjct: 481 TETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSP 540

Query: 541 SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 587
           SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS
Sbjct: 541 SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 586

BLAST of Csa6G020110 vs. NCBI nr
Match: gi|659128747|ref|XP_008464351.1| (PREDICTED: uncharacterized protein LOC103502259 [Cucumis melo])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 558/586 (95.22%), Postives = 567/586 (96.76%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELIQVQMLFGAKYGKEF+SAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELIQVQMLFGAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240
           LDWNPAETEAEFNKSPEDLLNG   FVG SKLPLPQEKHNETSNTTTDLASGPQPDSDSE
Sbjct: 181 LDWNPAETEAEFNKSPEDLLNG---FVGTSKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS 300
           LDMLDFPEVPKMSVCPQPTIDAGSAPSMI PPSVSP  ETD ASF+YSGIPESPPQNLHS
Sbjct: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMILPPSVSPQPETDRASFKYSGIPESPPQNLHS 300

Query: 301 RHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEK 360
           RH EVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFS RQT+LSP SDPTTPEEK
Sbjct: 301 RHGEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSHRQTDLSPPSDPTTPEEK 360

Query: 361 FDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQD 420
           FDIKPRI EEINSPLPSVSRASEE++GFKPRF+ EINSTPSSVSRTM EVNADL VDLQD
Sbjct: 361 FDIKPRITEEINSPLPSVSRASEEQFGFKPRFDSEINSTPSSVSRTMCEVNADLSVDLQD 420

Query: 421 VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADH 480
           VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHG TASPA H
Sbjct: 421 VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGATASPAGH 480

Query: 481 TETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSP 540
           TETYKLNQQDSLG+HSS+MPYYSQDS QEHHKLSRDPEVSDHSQL+PEKSSFD SPGNSP
Sbjct: 481 TETYKLNQQDSLGHHSSSMPYYSQDSSQEHHKLSRDPEVSDHSQLEPEKSSFDSSPGNSP 540

Query: 541 SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 587
           SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS
Sbjct: 541 SEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 583

BLAST of Csa6G020110 vs. NCBI nr
Match: gi|657984635|ref|XP_008384411.1| (PREDICTED: uncharacterized protein LOC103447017 [Malus domestica])

HSP 1 Score: 558.9 bits (1439), Expect = 1.0e-155
Identity = 335/599 (55.93%), Postives = 397/599 (66.28%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML+SFFNKGFKA +CKTLLKLT PRIKLLRNRREIQ+KQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLDSFFNKGFKAVKCKTLLKLTTPRIKLLRNRREIQIKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAA EILELFCELI VRLPIIE+QRECP+DLKEAISSVCFAAPRCADL 
Sbjct: 61  RVEHIIREENMMAAHEILELFCELIAVRLPIIESQRECPIDLKEAISSVCFAAPRCADLP 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL+QVQMLF AKYGKEF++AATEL+P+CGVNRQLIELLSVR PSPEKKLKLLKEIAEEH 
Sbjct: 121 ELLQVQMLFAAKYGKEFVAAATELIPDCGVNRQLIELLSVRPPSPEKKLKLLKEIAEEHG 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240
           LDW+PA +EAE  K  EDLL+G TQFV  SKLPLPQEKH+ET     D A     DSD  
Sbjct: 181 LDWDPAASEAEIYKPHEDLLSGPTQFVSGSKLPLPQEKHDETLQYAPDQAQKEPSDSDDX 240

Query: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS 300
           LD LDFPEVPK S+   P+ +  SAP M PP    P  E D    +  G  E+  +    
Sbjct: 241 LDSLDFPEVPKQSL--GPSTNPASAPMMPPPLPTHPHPEIDQDLSKKCGSIENQSEEXSF 300

Query: 301 RHEEVTLVRSVSPSNDQMNVSVG--EDKQFLPFITPPSLSP-SFSRRQTELSPSSDPTTP 360
              EV   RSV+ + +Q ++SVG  EDKQFLPFI+ PSLS  SF  RQ+   PS   T  
Sbjct: 301 DSGEVMEERSVANNQEQSHLSVGGVEDKQFLPFISAPSLSSVSFPTRQSNPPPSLSRTRS 360

Query: 361 EEKFDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSS----VSRTMSEVNAD 420
           E   D++  +     +   S  RA+          +  IN         V +  SE    
Sbjct: 361 EVNVDLQD-VLAAAQAAADSAERAATAARSAASLAQVRINEITKKNSEVVPKNWSESENP 420

Query: 421 LGVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPE--ISCENPFH 480
             VD+ D  +A QAAA++AERAA AA SAASL +V+I+E+TKK +++  E     ENPFH
Sbjct: 421 FHVDIPD-QSATQAAADSAERAATAAPSAASLXQVKINEITKKNSEEASENRSESENPFH 480

Query: 481 GGTASPADHTE----TYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQ--L 540
               + +D  E     + +    S G  +S +P+       + +   +  E+S+ S   +
Sbjct: 481 EDVPNQSDAREKPDFDHHIPSGGSDGVMNSPVPH-------QIYLYGQGSEISNASSIXM 540

Query: 541 KPEKSSFDYS-PGNSPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSA 584
           +P  +SFD   P +   EH+ V  HQPQRL SMDDDPYFSYPNLFTSQ  N G    SA
Sbjct: 541 EPLNASFDSPLPKDHAYEHEPVR-HQPQRLSSMDDDPYFSYPNLFTSQNSNVGSHAQSA 587

BLAST of Csa6G020110 vs. NCBI nr
Match: gi|743887606|ref|XP_011038197.1| (PREDICTED: IST1 homolog [Populus euphratica])

HSP 1 Score: 557.8 bits (1436), Expect = 2.3e-155
Identity = 333/597 (55.78%), Postives = 399/597 (66.83%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSM++SFFNKGFKAA+CKTLLKLTIPRIKLLRNRREIQ+K MRRDIAKLLETGQEATARI
Sbjct: 1   MSMIDSFFNKGFKAAKCKTLLKLTIPRIKLLRNRREIQMKHMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEI+ELFCELI VRLPIIETQRECPLDLKEAISSVCFAAPRCADL 
Sbjct: 61  RVEHIIREENMMAAQEIIELFCELISVRLPIIETQRECPLDLKEAISSVCFAAPRCADLP 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL QVQ+LF +KYGKEF++AATELMP+CGVNRQLIELLSVRAPSPEKKLKLLKEIAEEH+
Sbjct: 121 ELQQVQILFASKYGKEFVAAATELMPSCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHE 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVG---------ASKLPLPQEKHNETSNTTTDLAS 240
           LDW+PA +E E  K  EDLLNG T              S LPLP+EKH+E  N + D A 
Sbjct: 181 LDWDPAASETELLKKHEDLLNGPTHITDPTPVAGPTHISMLPLPKEKHDEVLN-SPDHAE 240

Query: 241 GPQPDSDSELDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIP 300
             QP+SD++ D LDFPEVPK+S  P  + ++  AP + PP   +   + DH S  +S   
Sbjct: 241 HEQPNSDTDFDPLDFPEVPKVS--PWLSANSALAPEIAPPAQAALHPDIDHESSNHSVTH 300

Query: 301 ESPPQNLHSRHEEVTLVRSVSPSNDQMNVSVG--EDKQFLPFITPPSL-SPSFSRRQTEL 360
           +   Q  H    +V   +SV+  ++  + +V   E+KQFLPFI PPSL S SFS   +  
Sbjct: 301 KDLSQEPHFEQGDVIENKSVATRDEISSTAVSDREEKQFLPFICPPSLSSESFSAIPSTS 360

Query: 361 SPSSDPTTPEEKFDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMS 420
            PS+  T  E   D++  +   +   + +   A+              +++P SV RT +
Sbjct: 361 PPSASRTESEANLDLQ-SVSAAVQGTVETAKHAAIP------------STSPPSVMRTRT 420

Query: 421 EVNADLGVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCEN 480
           E N    VDLQDVLAAA AAAE+AERAAAAARSAA+LA+ RI ELTKK ++K PE   +N
Sbjct: 421 EAN----VDLQDVLAAAHAAAESAERAAAAARSAATLAQARISELTKKNSEKFPE--DDN 480

Query: 481 PFHGGTASPADHTETYKLNQQDSLGYHSSAMPYYSQDSFQEH--HKLSRDPEVSDHSQLK 540
           PF   T+  +  TE      Q S G   S       ++   H  HK  +  E+ D    +
Sbjct: 481 PFFVDTSDHSASTERPHFVHQQSFGDPGSV-----PNNVDSHRPHKDHQASELLDIPSFE 540

Query: 541 PEKSSFDYSPGNSPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSA 584
             +  +D SP +     Q    HQPQRLPSMDD+PYFSYPNLFTSQ  N G    SA
Sbjct: 541 KLEVYYD-SPRSDHVVEQGSVSHQPQRLPSMDDEPYFSYPNLFTSQNSNLGSGAQSA 569

BLAST of Csa6G020110 vs. NCBI nr
Match: gi|645263685|ref|XP_008237348.1| (PREDICTED: IST1 homolog [Prunus mume])

HSP 1 Score: 542.7 bits (1397), Expect = 7.7e-151
Identity = 330/584 (56.51%), Postives = 387/584 (66.27%), Query Frame = 1

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML+SFFNKGFKAA+CKTLLKLTIPRIKLLRNRREIQ+KQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLLRNRREIQIKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAA EILELFCELI VRLPIIE+QRECPLDLKEAISSVCFAAPRCADL+
Sbjct: 61  RVEHIIREENMMAAHEILELFCELIAVRLPIIESQRECPLDLKEAISSVCFAAPRCADLS 120

Query: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL+QVQMLF +KYGKEF+SAATEL+P+CGVNRQLIELLSVRAPSPEKKLKLLKEIAEEH+
Sbjct: 121 ELLQVQMLFASKYGKEFVSAATELIPDCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHE 180

Query: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240
           LDW+PA +EAE  K  EDLLNG TQFV  SKLPLP+EKH+E S+   D +   Q DSD  
Sbjct: 181 LDWDPAASEAEIFKPHEDLLNGPTQFVSGSKLPLPKEKHDEPSHCAPDQSQKEQSDSDDG 240

Query: 241 LDMLDFPEVPKMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHS 300
           LD LDFPEVP +S+ P                 ++ P          +  P  PP     
Sbjct: 241 LDSLDFPEVPTVSLRP-----------------IANP----------ASAPAMPPPLPTP 300

Query: 301 RHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEK 360
            H EV    S          ++    + L F +  ++       + E S  S     +++
Sbjct: 301 PHPEVDHGLSKDSG------AIENPSEELSFESEEAMQARSVANKHEQSHVSVGGVEDKQ 360

Query: 361 FDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQD 420
           F   P I     SP PS+S AS         F    ++ P S+SRT S+ N    VD QD
Sbjct: 361 F--LPFI-----SP-PSLSSAS---------FSTRRSNPPPSLSRTRSDAN----VDFQD 420

Query: 421 VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADH 480
           VLAAAQAAA++AERAAAAARSAASLA+VRI+ELTKK +++ PE SCENPFH    S +D 
Sbjct: 421 VLAAAQAAADSAERAAAAARSAASLAQVRINELTKKNSEEVPENSCENPFHEDIPSESDT 480

Query: 481 TE----TYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQ--LKPEKSSFDY 540
            E     + +   DS G   S           + H+     +VSD +   ++P  +  D 
Sbjct: 481 REKPDFDHAIRNGDSDGVIHSL-------GLHQVHEYGHGAKVSDLNSVTMEPLNADLDS 522

Query: 541 S-PGNSPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPG 578
           S P +   EH+ V  HQPQRL SM+DDPYFSYPNLFTSQ  N G
Sbjct: 541 SLPNDHAFEHEPVR-HQPQRLSSMEDDPYFSYPNLFTSQNSNAG 522

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
IST1_MOUSE1.4e-2237.64IST1 homolog OS=Mus musculus GN=Ist1 PE=1 SV=1[more]
IST1_HUMAN1.4e-2237.64IST1 homolog OS=Homo sapiens GN=IST1 PE=1 SV=1[more]
IST1_RAT1.4e-2237.64IST1 homolog OS=Rattus norvegicus GN=Ist1 PE=2 SV=1[more]
IST1L_DICDI2.3e-2229.46IST1-like protein OS=Dictyostelium discoideum GN=DDB_G0289029 PE=3 SV=1[more]
IST1_BOVIN6.8e-2237.08IST1 homolog OS=Bos taurus GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KBZ0_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G020110 PE=4 SV=1[more]
A0A072U868_MEDTR9.2e-15155.43Regulator of Vps4 activity in the MVB pathway protein OS=Medicago truncatula GN=... [more]
A0A059BY61_EUCGR2.5e-14855.13Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F04150 PE=4 SV=1[more]
A0A061FL82_THECC3.5e-14253.38Regulator of Vps4 activity in the MVB pathway protein OS=Theobroma cacao GN=TCM_... [more]
A0A164X3F8_DAUCA1.1e-14053.07Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_019987 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G34220.11.9e-11041.48 Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G35730.16.0e-6940.40 Regulator of Vps4 activity in the MVB pathway protein[more]
AT1G25420.11.4e-6557.56 Regulator of Vps4 activity in the MVB pathway protein[more]
AT2G19710.13.2e-5448.33 Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G29440.12.0e-4845.00 Regulator of Vps4 activity in the MVB pathway protein[more]
Match NameE-valueIdentityDescription
gi|449441181|ref|XP_004138362.1|0.0e+00100.00PREDICTED: uncharacterized protein LOC101208940 [Cucumis sativus][more]
gi|659128747|ref|XP_008464351.1|0.0e+0095.22PREDICTED: uncharacterized protein LOC103502259 [Cucumis melo][more]
gi|657984635|ref|XP_008384411.1|1.0e-15555.93PREDICTED: uncharacterized protein LOC103447017 [Malus domestica][more]
gi|743887606|ref|XP_011038197.1|2.3e-15555.78PREDICTED: IST1 homolog [Populus euphratica][more]
gi|645263685|ref|XP_008237348.1|7.7e-15156.51PREDICTED: IST1 homolog [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005061Ist1
Vocabulary: Biological Process
TermDefinition
GO:0015031protein transport
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU108869cucumber EST collection version 3.0transcribed_cluster
CU116583cucumber EST collection version 3.0transcribed_cluster
CU144443cucumber EST collection version 3.0transcribed_cluster
CU161514cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G020110.1Csa6G020110.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU144443CU144443transcribed_cluster
CU108869CU108869transcribed_cluster
CU161514CU161514transcribed_cluster
CU116583CU116583transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161UNCHARACTERIZED DUF292coord: 386..577
score: 1.2E-235coord: 1..264
score: 1.2E
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 18..181
score: 1.8
NoneNo IPR availableunknownCoilCoilcoord: 32..59
score: -coord: 422..442
scor
NoneNo IPR availablePANTHERPTHR12161:SF21REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 386..577
score: 1.2E-235coord: 1..264
score: 1.2E