Csa6G005130 (gene) Cucumber (Chinese Long) v2

NameCsa6G005130
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPentatricopeptide repeat-containing protein; contains IPR002885 (Pentatricopeptide repeat)
LocationChr6 : 452515 .. 454599 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGATTTGGACGTCGACCCACTTTGGTCGTTCTCGTTTGGTCCATTCCTTTTCCTTCAACGTCCTGAAAGCCGCCGCCCCGGTGAATTCCATTCCCCATGATACCCTTTTGCACAGCCTTGTTGTGAAGTTGGGATTGGTTAATGAACTGTCTGTACAGAACAAGCTATTGAGAGTTTATGTTAAGTGCAGAGATTTGGACAGTGCGCGGAACCTGTTTGATGAAATGGCTAGGAGAAATGTTGTGTCGTGGAATACGGTGATATGTGGGCTTGTCGATGGCGGGTATGGAGGTGAGTTTAAAATGAGGCAGCACTCGATTTTTTTATATTTTAAGAAGATGTTGATGGGTTTGGTGGACCCAGATGGCATCACGTTTAATGGGTTGTTTCGATCTTGTGTTGTGTTGAATGATGTTGAGAGTGGCAGGCAATTGCATAGTTTTGTTATGAAAATTGGGTTTGATTTGGATTGTTTTGTGGGGAGTGCAGTGGTCGATTTTTATGCGAAATGTGGGTTATATGAAGATGCGAGATTAGCGTTTAGCTGTATTCTGTATAGGGATCTGGTTTTGTGGAATGTGATGTTGTACTGTTGTGTGTTTAATTCTTTGAGCCGAGAAGCGATTGAAGTCTTTCGTTTGATGCAGTTGGAAGGCTTTAAAGGTGATGATTTTACATTCAGCAGCCTGCTAAGTTCGTGCAAGTATAAAGGATCAGGAGAATTGGGTAAGCAACTACATTGTCTTCTTATAAAACAGTCATTTGATTTAGATATTCTTGTGGCAAGTTCACTTGTCAATGTGTATACTAAAAACGATAATTTATATGATGCTCGCAAGGTTTTTGATGAAATGCCAACTAGAAATTCCGTGTCTTGGACCACTATGATTGTGGGGTATGGACAGCACGAATATGGAAAAGAGGCAGTGAAACTGTTCAGGAGAATGTTTAGGAAAGATTATTGCCCGGATGAGTTAACTTTTGCTAGTGTGCTGAGCTCGTGTGGCTTTACGTCTGGGGCCTCTGAACTGATGCAAGTTCATTCCTGCTTGATAAAACTCGGTTTTGAAGCATTTTTGTCTATTAACAATGGTTTGATATATGCATACTCAAAGTGTGGTATAATTGCTGCAGCGTTACAATGCTTTAGATTAATTGCAGAACCAGATTTGGTAACATGGACATCAATTATATGTGGACTTGCACTTTGTGGCCTTGAGAAAGATGCTGTTAAGTTATTTGATAAGATGTTATCGTATGGCATTAGACCAGATAAAATTGCATTCCTTGGAGTTCTCTCTGCCTGTAGTCATGGGGGATTTGTAAGCATGGGGCTTCACTACTTCAATTTAATGACGAACCAATACCAACTTGTTCCTGATTCAGAGCATTTAACATGCTTGATCGACCTTCTTGGTAGAGCGGGTAGTCTAGACCAGGCTTTTGATCTTTTGAAATCAATGCCAAAGGAAGCTGGACCAGATGCTTTAAGGGCTTTCATTCGAGCATGTAGAACTCATGGGAACTTGAGATTAGCTAAACGGGCAATGGAATTTGCATCAGAGCCAGATGAACCAGTGAACTATTCCCTAGTGTCGAATATGTATGCTTCTGAAGGAAGATGGTCAGATGTGGCAAGAATGCGCAAACTGATTAACGATCGTTGTGAACAGAAAACCCCAGGCCTTAGTTGGGTAGAAATTGCTGGTTATAACCACTTGTTTATATCAGGTGATAGATCCCATCCACAGTCTTTAGATCTCTATGCCATGCTAGGATTATTACTAAACACGATGAAGAAGGATTACAAGTTCACAGCCTCTCAGGTAGATATTGTGCCCGAATGAATGCCAAACCTTGGATGATCTCAGGTTGGACACAAATCAAAATCCATTTCAAGGTCCAGCACTTGACCGGCTACACATGACTTCAATTGGTACATCTAATCAGCCATAGGTACATGATTTCAGATACTAAAAAAAAGTGTTTGGTATTAGGAATCAAAATAAAGTAAGTTGAAATTTGATGTTTTCTCTGTTTATAGCATTGTAGGTGAGCCCACTGTTGTAAAGTAGCAAAGT

mRNA sequence

ATGCTGATTTGGACGTCGACCCACTTTGGTCGTTCTCGTTTGGTCCATTCCTTTTCCTTCAACGTCCTGAAAGCCGCCGCCCCGGTGAATTCCATTCCCCATGATACCCTTTTGCACAGCCTTGTTGTGAAGTTGGGATTGGTTAATGAACTGTCTGTACAGAACAAGCTATTGAGAGTTTATGTTAAGTGCAGAGATTTGGACAGTGCGCGGAACCTGTTTGATGAAATGGCTAGGAGAAATGTTGTGTCGTGGAATACGGTGATATGTGGGCTTGTCGATGGCGGGTATGGAGGTGAGTTTAAAATGAGGCAGCACTCGATTTTTTTATATTTTAAGAAGATGTTGATGGGTTTGGTGGACCCAGATGGCATCACGTTTAATGGGTTGTTTCGATCTTGTGTTGTGTTGAATGATGTTGAGAGTGGCAGGCAATTGCATAGTTTTGTTATGAAAATTGGGTTTGATTTGGATTGTTTTGTGGGGAGTGCAGTGGTCGATTTTTATGCGAAATGTGGGTTATATGAAGATGCGAGATTAGCGTTTAGCTGTATTCTGTATAGGGATCTGGTTTTGTGGAATGTGATGTTGTACTGTTGTGTGTTTAATTCTTTGAGCCGAGAAGCGATTGAAGTCTTTCGTTTGATGCAGTTGGAAGGCTTTAAAGGTGATGATTTTACATTCAGCAGCCTGCTAAGTTCGTGCAAGTATAAAGGATCAGGAGAATTGGGTAAGCAACTACATTGTCTTCTTATAAAACAGTCATTTGATTTAGATATTCTTGTGGCAAGTTCACTTGTCAATGTGTATACTAAAAACGATAATTTATATGATGCTCGCAAGGTTTTTGATGAAATGCCAACTAGAAATTCCGTGTCTTGGACCACTATGATTGTGGGGTATGGACAGCACGAATATGGAAAAGAGGCAGTGAAACTGTTCAGGAGAATGTTTAGGAAAGATTATTGCCCGGATGAGTTAACTTTTGCTAGTGTGCTGAGCTCGTGTGGCTTTACGTCTGGGGCCTCTGAACTGATGCAAGTTCATTCCTGCTTGATAAAACTCGGTTTTGAAGCATTTTTGTCTATTAACAATGGTTTGATATATGCATACTCAAAGTGTGGTATAATTGCTGCAGCGTTACAATGCTTTAGATTAATTGCAGAACCAGATTTGGTAACATGGACATCAATTATATGTGGACTTGCACTTTGTGGCCTTGAGAAAGATGCTGTTAAGTTATTTGATAAGATGTTATCGTATGGCATTAGACCAGATAAAATTGCATTCCTTGGAGTTCTCTCTGCCTGTAGTCATGGGGGATTTGTAAGCATGGGGCTTCACTACTTCAATTTAATGACGAACCAATACCAACTTGTTCCTGATTCAGAGCATTTAACATGCTTGATCGACCTTCTTGGTAGAGCGGGTAGTCTAGACCAGGCTTTTGATCTTTTGAAATCAATGCCAAAGGAAGCTGGACCAGATGCTTTAAGGGCTTTCATTCGAGCATGTAGAACTCATGGGAACTTGAGATTAGCTAAACGGGCAATGGAATTTGCATCAGAGCCAGATGAACCAGTGAACTATTCCCTAGTGTCGAATATGTATGCTTCTGAAGGAAGATGGTCAGATGTGGCAAGAATGCGCAAACTGATTAACGATCGTTGTGAACAGAAAACCCCAGGCCTTAGTTGGGTAGAAATTGCTGGTTATAACCACTTGTTTATATCAGGTGATAGATCCCATCCACAGTCTTTAGATCTCTATGCCATGCTAGGATTATTACTAAACACGATGAAGAAGGATTACAAGTTCACAGCCTCTCAGGTAGATATTGTGCCCGAATGA

Coding sequence (CDS)

ATGCTGATTTGGACGTCGACCCACTTTGGTCGTTCTCGTTTGGTCCATTCCTTTTCCTTCAACGTCCTGAAAGCCGCCGCCCCGGTGAATTCCATTCCCCATGATACCCTTTTGCACAGCCTTGTTGTGAAGTTGGGATTGGTTAATGAACTGTCTGTACAGAACAAGCTATTGAGAGTTTATGTTAAGTGCAGAGATTTGGACAGTGCGCGGAACCTGTTTGATGAAATGGCTAGGAGAAATGTTGTGTCGTGGAATACGGTGATATGTGGGCTTGTCGATGGCGGGTATGGAGGTGAGTTTAAAATGAGGCAGCACTCGATTTTTTTATATTTTAAGAAGATGTTGATGGGTTTGGTGGACCCAGATGGCATCACGTTTAATGGGTTGTTTCGATCTTGTGTTGTGTTGAATGATGTTGAGAGTGGCAGGCAATTGCATAGTTTTGTTATGAAAATTGGGTTTGATTTGGATTGTTTTGTGGGGAGTGCAGTGGTCGATTTTTATGCGAAATGTGGGTTATATGAAGATGCGAGATTAGCGTTTAGCTGTATTCTGTATAGGGATCTGGTTTTGTGGAATGTGATGTTGTACTGTTGTGTGTTTAATTCTTTGAGCCGAGAAGCGATTGAAGTCTTTCGTTTGATGCAGTTGGAAGGCTTTAAAGGTGATGATTTTACATTCAGCAGCCTGCTAAGTTCGTGCAAGTATAAAGGATCAGGAGAATTGGGTAAGCAACTACATTGTCTTCTTATAAAACAGTCATTTGATTTAGATATTCTTGTGGCAAGTTCACTTGTCAATGTGTATACTAAAAACGATAATTTATATGATGCTCGCAAGGTTTTTGATGAAATGCCAACTAGAAATTCCGTGTCTTGGACCACTATGATTGTGGGGTATGGACAGCACGAATATGGAAAAGAGGCAGTGAAACTGTTCAGGAGAATGTTTAGGAAAGATTATTGCCCGGATGAGTTAACTTTTGCTAGTGTGCTGAGCTCGTGTGGCTTTACGTCTGGGGCCTCTGAACTGATGCAAGTTCATTCCTGCTTGATAAAACTCGGTTTTGAAGCATTTTTGTCTATTAACAATGGTTTGATATATGCATACTCAAAGTGTGGTATAATTGCTGCAGCGTTACAATGCTTTAGATTAATTGCAGAACCAGATTTGGTAACATGGACATCAATTATATGTGGACTTGCACTTTGTGGCCTTGAGAAAGATGCTGTTAAGTTATTTGATAAGATGTTATCGTATGGCATTAGACCAGATAAAATTGCATTCCTTGGAGTTCTCTCTGCCTGTAGTCATGGGGGATTTGTAAGCATGGGGCTTCACTACTTCAATTTAATGACGAACCAATACCAACTTGTTCCTGATTCAGAGCATTTAACATGCTTGATCGACCTTCTTGGTAGAGCGGGTAGTCTAGACCAGGCTTTTGATCTTTTGAAATCAATGCCAAAGGAAGCTGGACCAGATGCTTTAAGGGCTTTCATTCGAGCATGTAGAACTCATGGGAACTTGAGATTAGCTAAACGGGCAATGGAATTTGCATCAGAGCCAGATGAACCAGTGAACTATTCCCTAGTGTCGAATATGTATGCTTCTGAAGGAAGATGGTCAGATGTGGCAAGAATGCGCAAACTGATTAACGATCGTTGTGAACAGAAAACCCCAGGCCTTAGTTGGGTAGAAATTGCTGGTTATAACCACTTGTTTATATCAGGTGATAGATCCCATCCACAGTCTTTAGATCTCTATGCCATGCTAGGATTATTACTAAACACGATGAAGAAGGATTACAAGTTCACAGCCTCTCAGGTAGATATTGTGCCCGAATGA

Protein sequence

MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTMKKDYKFTASQVDIVPE*
BLAST of Csa6G005130 vs. Swiss-Prot
Match: PP203_ARATH (Pentatricopeptide repeat-containing protein At2g46050, mitochondrial OS=Arabidopsis thaliana GN=PCMP-E39 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.4e-126
Identity = 250/554 (45.13%), Postives = 342/554 (61.73%), Query Frame = 1

Query: 21  NVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARR 80
           +V K +A ++ +      H  +VK G+ N L +QNKLL+ Y K R+ D A  LFDEM  R
Sbjct: 41  SVSKLSASLDHLSDVKQEHGFMVKQGIYNSLFLQNKLLQAYTKIREFDDADKLFDEMPLR 100

Query: 81  NVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDV 140
           N+V+WN +I G++     G+   R H  F Y  ++L   V  D ++F GL R C    ++
Sbjct: 101 NIVTWNILIHGVIQRD--GDTNHRAHLGFCYLSRILFTDVSLDHVSFMGLIRLCTDSTNM 160

Query: 141 ESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCC 200
           ++G QLH  ++K G +  CF  +++V FY KCGL  +AR  F  +L RDLVLWN ++   
Sbjct: 161 KAGIQLHCLMVKQGLESSCFPSTSLVHFYGKCGLIVEARRVFEAVLDRDLVLWNALVSSY 220

Query: 201 VFNSLSREAIEVFRLMQLEG--FKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDL 260
           V N +  EA  + +LM  +   F+GD FTFSSLLS+C+     E GKQ+H +L K S+  
Sbjct: 221 VLNGMIDEAFGLLKLMGSDKNRFRGDYFTFSSLLSACRI----EQGKQIHAILFKVSYQF 280

Query: 261 DILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMF 320
           DI VA++L+N+Y K+++L DAR+ F+ M  RN VSW  MIVG+ Q+  G+EA++LF +M 
Sbjct: 281 DIPVATALLNMYAKSNHLSDARECFESMVVRNVVSWNAMIVGFAQNGEGREAMRLFGQML 340

Query: 321 RKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIA 380
            ++  PDELTFASVLSSC   S   E+ QV + + K G   FLS+ N LI +YS+ G ++
Sbjct: 341 LENLQPDELTFASVLSSCAKFSAIWEIKQVQAMVTKKGSADFLSVANSLISSYSRNGNLS 400

Query: 381 AALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACS 440
            AL CF  I EPDLV+WTS+I  LA  G  ++++++F+ ML   ++PDKI FL VLSACS
Sbjct: 401 EALLCFHSIREPDLVSWTSVIGALASHGFAEESLQMFESMLQ-KLQPDKITFLEVLSACS 460

Query: 441 HGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDAL 500
           HGG V  GL  F  MT  Y++  + EH TCLIDLLGRAG +D+A D+L SMP E    AL
Sbjct: 461 HGGLVQEGLRCFKRMTEFYKIEAEDEHYTCLIDLLGRAGFIDEASDVLNSMPTEPSTHAL 520

Query: 501 RAFIRACRTHGNLRL----AKRAMEFASEPDEPVNYSLVSNMYASEGRWSDVARMRKLIN 560
            AF   C  H         AK+ +E   EP +PVNYS++SN Y SEG W+  A +RK   
Sbjct: 521 AAFTGGCNIHEKRESMKWGAKKLLEI--EPTKPVNYSILSNAYVSEGHWNQAALLRKRER 580

Query: 561 DRC-EQKTPGLSWV 568
             C   KTPG SW+
Sbjct: 581 RNCYNPKTPGCSWL 585

BLAST of Csa6G005130 vs. Swiss-Prot
Match: PP151_ARATH (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana GN=PCMP-E76 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.0e-100
Identity = 207/634 (32.65%), Postives = 331/634 (52.21%), Query Frame = 1

Query: 38  LHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGY 97
           +H+ V+K G  NE+ +QN+L+  Y KC  L+  R +FD+M +RN+ +WN+V+ GL   G+
Sbjct: 42  VHASVIKSGFSNEIFIQNRLIDAYSKCGSLEDGRQVFDKMPQRNIYTWNSVVTGLTKLGF 101

Query: 98  GGE----FKMRQHSIFLYFKKMLMGLVDPDGI--------------------TFNGLFRS 157
             E    F+         +  M+ G    D                      +F  +  +
Sbjct: 102 LDEADSLFRSMPERDQCTWNSMVSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSA 161

Query: 158 CVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLW 217
           C  LND+  G Q+HS + K  F  D ++GSA+VD Y+KCG   DA+  F  +  R++V W
Sbjct: 162 CSGLNDMNKGVQVHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQRVFDEMGDRNVVSW 221

Query: 218 NVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIK 277
           N ++ C   N  + EA++VF++M     + D+ T +S++S+C    + ++G+++H  ++K
Sbjct: 222 NSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVK 281

Query: 278 QS-FDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNS--------------------- 337
                 DI+++++ V++Y K   + +AR +FD MP RN                      
Sbjct: 282 NDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAASTKAARL 341

Query: 338 ----------VSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSG 397
                     VSW  +I GY Q+   +EA+ LF  + R+  CP   +FA++L +C   + 
Sbjct: 342 MFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKACADLAE 401

Query: 398 ASELMQVHSCLIKLGF------EAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTW 457
               MQ H  ++K GF      E  + + N LI  Y KCG +      FR + E D V+W
Sbjct: 402 LHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMMERDCVSW 461

Query: 458 TSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTN 517
            ++I G A  G   +A++LF +ML  G +PD I  +GVLSAC H GFV  G HYF+ MT 
Sbjct: 462 NAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSACGHAGFVEEGRHYFSSMTR 521

Query: 518 QYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAK 577
            + + P  +H TC++DLLGRAG L++A  +++ MP +       + + AC+ H N+ L K
Sbjct: 522 DFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSVIWGSLLAACKVHRNITLGK 581

Query: 578 RAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDRCEQKTPGLSWVEIAGYN 608
              E     EP     Y L+SNMYA  G+W DV  +RK +      K PG SW++I G++
Sbjct: 582 YVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSMRKEGVTKQPGCSWIKIQGHD 641


HSP 2 Score: 120.9 bits (302), Expect = 4.8e-26
Identity = 82/313 (26.20%), Postives = 152/313 (48.56%), Query Frame = 1

Query: 179 RLAFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLE-GFKGDDFTFSSLLSSCKY 238
           +LA     + D   +  +L  C+ + LS   +       ++ GF  + F  + L+ +   
Sbjct: 8   KLAADLSSFTDSSPFAKLLDSCIKSKLSAIYVRYVHASVIKSGFSNEIFIQNRLIDAYSK 67

Query: 239 KGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTM 298
            GS E G+Q+   + +++    I   +S+V   TK   L +A  +F  MP R+  +W +M
Sbjct: 68  CGSLEDGRQVFDKMPQRN----IYTWNSVVTGLTKLGFLDEADSLFRSMPERDQCTWNSM 127

Query: 299 IVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGF 358
           + G+ QH+  +EA+  F  M ++ +  +E +FASVLS+C   +  ++ +QVHS + K  F
Sbjct: 128 VSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSACSGLNDMNKGVQVHSLIAKSPF 187

Query: 359 EAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDK 418
            + + I + L+  YSKCG +  A + F  + + ++V+W S+I      G   +A+ +F  
Sbjct: 188 LSDVYIGSALVDMYSKCGNVNDAQRVFDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQM 247

Query: 419 MLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAG 478
           ML   + PD++    V+SAC+    + +G      +    +L  D       +D+  +  
Sbjct: 248 MLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCS 307

Query: 479 SLDQAFDLLKSMP 491
            + +A  +  SMP
Sbjct: 308 RIKEARFIFDSMP 316


HSP 3 Score: 42.4 bits (98), Expect = 2.2e-02
Identity = 24/72 (33.33%), Postives = 40/72 (55.56%), Query Frame = 1

Query: 21  NVLKAAAPVNSIPHDTLLHSLVVKLG-LVNELSVQNKLLRVYVKCRDLDSARNLFDEMAR 80
           +V+ A A +++I     +H  VVK   L N++ + N  + +Y KC  +  AR +FD M  
Sbjct: 258 SVISACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPI 317

Query: 81  RNVVSWNTVICG 92
           RNV++  ++I G
Sbjct: 318 RNVIAETSMISG 329

BLAST of Csa6G005130 vs. Swiss-Prot
Match: PP320_ARATH (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana GN=DOT4 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 2.7e-93
Identity = 192/581 (33.05%), Postives = 325/581 (55.94%), Query Frame = 1

Query: 17  SFSFN-VLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFD 76
           S++F+ V K+ + + S+     LH  ++K G     SV N L+  Y+K + +DSAR +FD
Sbjct: 195 SYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFD 254

Query: 77  EMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCV 136
           EM  R+V+SWN++I G V  G       +  S+F+   +ML+  ++ D  T   +F  C 
Sbjct: 255 EMTERDVISWNSIINGYVSNGLAE----KGLSVFV---QMLVSGIEIDLATIVSVFAGCA 314

Query: 137 VLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNV 196
               +  GR +HS  +K  F  +    + ++D Y+KCG  + A+  F  +  R +V +  
Sbjct: 315 DSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTS 374

Query: 197 MLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQS 256
           M+       L+ EA+++F  M+ EG   D +T +++L+ C      + GK++H  + +  
Sbjct: 375 MIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKEND 434

Query: 257 FDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFR 316
              DI V+++L+++Y K  ++ +A  VF EM  ++ +SW T+I GY ++ Y  EA+ LF 
Sbjct: 435 LGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFN 494

Query: 317 RMFR-KDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKC 376
            +   K + PDE T A VL +C   S   +  ++H  +++ G+ +   + N L+  Y+KC
Sbjct: 495 LLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKC 554

Query: 377 GIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVL 436
           G +  A   F  IA  DLV+WT +I G  + G  K+A+ LF++M   GI  D+I+F+ +L
Sbjct: 555 GALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLL 614

Query: 437 SACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAG 496
            ACSH G V  G  +FN+M ++ ++ P  EH  C++D+L R G L +A+  +++MP    
Sbjct: 615 YACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPD 674

Query: 497 PDALRAFIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKL 556
                A +  CR H +++LA++  E  F  EP+    Y L++N+YA   +W  V R+RK 
Sbjct: 675 ATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKR 734

Query: 557 INDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAML 594
           I  R  +K PG SW+EI G  ++F++GD S+P++ ++ A L
Sbjct: 735 IGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFL 768


HSP 2 Score: 194.1 bits (492), Expect = 4.4e-48
Identity = 140/465 (30.11%), Postives = 227/465 (48.82%), Query Frame = 1

Query: 21  NVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARR 80
           +VL+  A   S+     + + +   G V + ++ +KL  +Y  C DL  A  +FDE+   
Sbjct: 99  SVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIE 158

Query: 81  NVVSWNTVICGLVDGG-YGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLND 140
             + WN ++  L   G + G   +        FKKM+   V+ D  TF+ + +S   L  
Sbjct: 159 KALFWNILMNELAKSGDFSGSIGL--------FKKMMSSGVEMDSYTFSCVSKSFSSLRS 218

Query: 141 VESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYC 200
           V  G QLH F++K GF     VG+++V FY K    + AR  F  +  RD++ WN ++  
Sbjct: 219 VHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIING 278

Query: 201 CVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLD 260
            V N L+ + + VF  M + G + D  T  S+ + C       LG+ +H + +K  F  +
Sbjct: 279 YVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSRE 338

Query: 261 ILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFR 320
               ++L+++Y+K  +L  A+ VF EM  R+ VS+T+MI GY +     EAVKLF  M  
Sbjct: 339 DRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEE 398

Query: 321 KDYCPDELTFASVLSSCGFTSGASELMQVHSCLIK--LGFEAFLSINNGLIYAYSKCGII 380
           +   PD  T  +VL+ C       E  +VH  + +  LGF+ F+S  N L+  Y+KCG +
Sbjct: 399 EGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVS--NALMDMYAKCGSM 458

Query: 381 AAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKML-SYGIRPDKIAFLGVLSA 440
             A   F  +   D+++W +II G +      +A+ LF+ +L      PD+     VL A
Sbjct: 459 QEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPA 518

Query: 441 CSHGGFVSMG--LHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSL 480
           C+       G  +H + +M N Y    D      L+D+  + G+L
Sbjct: 519 CASLSAFDKGREIHGY-IMRNGY--FSDRHVANSLVDMYAKCGAL 550


HSP 3 Score: 95.5 bits (236), Expect = 2.1e-18
Identity = 73/303 (24.09%), Postives = 131/303 (43.23%), Query Frame = 1

Query: 224 DDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVF 283
           D  T  S+L  C    S + GK++   +    F +D  + S L  +YT   +L +A +VF
Sbjct: 93  DPRTLCSVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVF 152

Query: 284 DEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGAS 343
           DE+    ++ W  ++    +      ++ LF++M       D  TF+ V  S        
Sbjct: 153 DEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVH 212

Query: 344 ELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLA 403
              Q+H  ++K GF    S+ N L+  Y K   + +A + F  + E D+++W SII G  
Sbjct: 213 GGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYV 272

Query: 404 LCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDS 463
             GL +  + +F +ML  GI  D    + V + C+    +S+G    ++         + 
Sbjct: 273 SNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKAC-FSRED 332

Query: 464 EHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASE 523
                L+D+  + G LD A    K++ +E    ++ ++      +    LA  A++   E
Sbjct: 333 RFCNTLLDMYSKCGDLDSA----KAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEE 390

Query: 524 PDE 527
            +E
Sbjct: 393 MEE 390

BLAST of Csa6G005130 vs. Swiss-Prot
Match: PP357_ARATH (Pentatricopeptide repeat-containing protein At4g39530 OS=Arabidopsis thaliana GN=PCMP-E52 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.6e-90
Identity = 197/581 (33.91%), Postives = 316/581 (54.39%), Query Frame = 1

Query: 22  VLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRN 81
           VL A + +  +     +H+ +++ GL  + S+ N L+  YVKC  + +A  LF+ M  +N
Sbjct: 255 VLSACSILPFLEGGKQIHAHILRYGLEMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKN 314

Query: 82  VVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVE 141
           ++SW T++ G           + + ++ L+      GL  PD    + +  SC  L+ + 
Sbjct: 315 IISWTTLLSGYKQNA------LHKEAMELFTSMSKFGL-KPDMYACSSILTSCASLHALG 374

Query: 142 SGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVML--YC 201
            G Q+H++ +K     D +V ++++D YAKC    DAR  F      D+VL+N M+  Y 
Sbjct: 375 FGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCLTDARKVFDIFAAADVVLFNAMIEGYS 434

Query: 202 CVFNSLS-REAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDL 261
            +       EA+ +FR M+    +    TF SLL +     S  L KQ+H L+ K   +L
Sbjct: 435 RLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLLRASASLTSLGLSKQIHGLMFKYGLNL 494

Query: 262 DILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMF 321
           DI   S+L++VY+    L D+R VFDEM  ++ V W +M  GY Q    +EA+ LF  + 
Sbjct: 495 DIFAGSALIDVYSNCYCLKDSRLVFDEMKVKDLVIWNSMFAGYVQQSENEEALNLFLELQ 554

Query: 322 RKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIA 381
                PDE TFA+++++ G  +      + H  L+K G E    I N L+  Y+KCG   
Sbjct: 555 LSRERPDEFTFANMVTAAGNLASVQLGQEFHCQLLKRGLECNPYITNALLDMYAKCGSPE 614

Query: 382 AALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACS 441
            A + F   A  D+V W S+I   A  G  K A+++ +KM+S GI P+ I F+GVLSACS
Sbjct: 615 DAHKAFDSAASRDVVCWNSVISSYANHGEGKKALQMLEKMMSEGIEPNYITFVGVLSACS 674

Query: 442 HGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDAL 501
           H G V  GL  F LM  ++ + P++EH  C++ LLGRAG L++A +L++ MP +      
Sbjct: 675 HAGLVEDGLKQFELML-RFGIEPETEHYVCMVSLLGRAGRLNKARELIEKMPTKPAAIVW 734

Query: 502 RAFIRACRTHGNLRLAKRAMEFA--SEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDR 561
           R+ +  C   GN+ LA+ A E A  S+P +  +++++SN+YAS+G W++  ++R+ +   
Sbjct: 735 RSLLSGCAKAGNVELAEHAAEMAILSDPKDSGSFTMLSNIYASKGMWTEAKKVRERMKVE 794

Query: 562 CEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLL 598
              K PG SW+ I    H+F+S D+SH ++  +Y +L  LL
Sbjct: 795 GVVKEPGRSWIGINKEVHIFLSKDKSHCKANQIYEVLDDLL 827


HSP 2 Score: 197.2 bits (500), Expect = 5.2e-49
Identity = 143/499 (28.66%), Postives = 237/499 (47.49%), Query Frame = 1

Query: 38  LHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGY 97
           L S +VK G   ++ V   L+  Y+K  ++D AR +FD +  ++ V+W T+I G V  G 
Sbjct: 170 LQSFLVKSGFDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMG- 229

Query: 98  GGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDL 157
                 R +     F +++   V PDG   + +  +C +L  +E G+Q+H+ +++ G ++
Sbjct: 230 ------RSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLEM 289

Query: 158 DCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQ 217
           D  + + ++D Y KCG    A   F+ +  ++++ W  +L     N+L +EA+E+F  M 
Sbjct: 290 DASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSMS 349

Query: 218 LEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLY 277
             G K D +  SS+L+SC    +   G Q+H   IK +   D  V +SL+++Y K D L 
Sbjct: 350 KFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCLT 409

Query: 278 DARKVFDEMPTRNSVSWTTMIVGY---GQHEYGKEAVKLFRRMFRKDYCPDELTFASVLS 337
           DARKVFD     + V +  MI GY   G      EA+ +FR M  +   P  LTF S+L 
Sbjct: 410 DARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLLR 469

Query: 338 SCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVT 397
           +    +      Q+H  + K G    +   + LI  YS C  +  +   F  +   DLV 
Sbjct: 470 ASASLTSLGLSKQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKDSRLVFDEMKVKDLVI 529

Query: 398 WTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMT 457
           W S+  G       ++A+ LF ++     RPD+  F  +++A  +   V +G   F+   
Sbjct: 530 WNSMFAGYVQQSENEEALNLFLELQLSRERPDEFTFANMVTAAGNLASVQLG-QEFHCQL 589

Query: 458 NQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDAL--RAFIRACRTHGNLR 517
            +  L  +      L+D+  + GS + A    K+    A  D +   + I +   HG  +
Sbjct: 590 LKRGLECNPYITNALLDMYAKCGSPEDAH---KAFDSAASRDVVCWNSVISSYANHGEGK 649

Query: 518 LAKRAME-FASEPDEPVNY 531
            A + +E   SE  EP NY
Sbjct: 650 KALQMLEKMMSEGIEP-NY 656


HSP 3 Score: 191.4 bits (485), Expect = 2.9e-47
Identity = 122/433 (28.18%), Postives = 217/433 (50.12%), Query Frame = 1

Query: 22  VLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRN 81
           +L+  A  + + +  ++H  ++  GL  +  + N L+ +Y +   +  AR +F++M  RN
Sbjct: 50  LLQLRASDDLLHYQNVVHGQIIVWGLELDTYLSNILINLYSRAGGMVYARKVFEKMPERN 109

Query: 82  VVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVE 141
           +VSW+T++      G      + + S+ ++ +        P+    +   ++C  L+   
Sbjct: 110 LVSWSTMVSACNHHG------IYEESLVVFLEFWRTRKDSPNEYILSSFIQACSGLDG-- 169

Query: 142 SGR----QLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVML 201
            GR    QL SF++K GFD D +VG+ ++DFY K G  + ARL F  +  +  V W  M+
Sbjct: 170 RGRWMVFQLQSFLVKSGFDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMI 229

Query: 202 YCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFD 261
             CV    S  ++++F  +  +    D +  S++LS+C      E GKQ+H  +++   +
Sbjct: 230 SGCVKMGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLE 289

Query: 262 LDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRM 321
           +D  + + L++ Y K   +  A K+F+ MP +N +SWTT++ GY Q+   KEA++LF  M
Sbjct: 290 MDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSM 349

Query: 322 FRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGII 381
            +    PD    +S+L+SC          QVH+  IK        + N LI  Y+KC  +
Sbjct: 350 SKFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCL 409

Query: 382 AAALQCFRLIAEPDLVTWTSIICGLALCGLE---KDAVKLFDKMLSYGIRPDKIAFLGVL 441
             A + F + A  D+V + ++I G +  G +    +A+ +F  M    IRP  + F+ +L
Sbjct: 410 TDARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLL 469

Query: 442 SACSHGGFVSMGL 448
            A +     S+GL
Sbjct: 470 RASA--SLTSLGL 472


HSP 4 Score: 162.2 bits (409), Expect = 1.9e-38
Identity = 97/342 (28.36%), Postives = 177/342 (51.75%), Query Frame = 1

Query: 146 LHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCCVFNSL 205
           +H  ++  G +LD ++ + +++ Y++ G    AR  F  +  R+LV W+ M+  C  + +
Sbjct: 66  VHGQIIVWGLELDTYLSNILINLYSRAGGMVYARKVFEKMPERNLVSWSTMVSACNHHGI 125

Query: 206 SREAIEVF-RLMQLEGFKGDDFTFSSLLSSCK-YKGSGE-LGKQLHCLLIKQSFDLDILV 265
             E++ VF    +      +++  SS + +C    G G  +  QL   L+K  FD D+ V
Sbjct: 126 YEESLVVFLEFWRTRKDSPNEYILSSFIQACSGLDGRGRWMVFQLQSFLVKSGFDRDVYV 185

Query: 266 ASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDY 325
            + L++ Y K+ N+  AR VFD +P +++V+WTTMI G  +      +++LF ++   + 
Sbjct: 186 GTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMGRSYVSLQLFYQLMEDNV 245

Query: 326 CPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQ 385
            PD    ++VLS+C          Q+H+ +++ G E   S+ N LI +Y KCG + AA +
Sbjct: 246 VPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLEMDASLMNVLIDSYVKCGRVIAAHK 305

Query: 386 CFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGF 445
            F  +   ++++WT+++ G     L K+A++LF  M  +G++PD  A   +L++C+    
Sbjct: 306 LFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSMSKFGLKPDMYACSSILTSCASLHA 365

Query: 446 VSMG--LHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQA 483
           +  G  +H + +  N   L  DS     LID+  +   L  A
Sbjct: 366 LGFGTQVHAYTIKAN---LGNDSYVTNSLIDMYAKCDCLTDA 404


HSP 5 Score: 112.8 bits (281), Expect = 1.3e-23
Identity = 89/311 (28.62%), Postives = 145/311 (46.62%), Query Frame = 1

Query: 247 LHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEY 306
           +H  +I    +LD  +++ L+N+Y++   +  ARKVF++MP RN VSW+TM+     H  
Sbjct: 66  VHGQIIVWGLELDTYLSNILINLYSRAGGMVYARKVFEKMPERNLVSWSTMVSACNHHGI 125

Query: 307 GKEAVKLFRRMFR-KDYCPDELTFASVLSSCGFTSGASELM--QVHSCLIKLGFEAFLSI 366
            +E++ +F   +R +   P+E   +S + +C    G    M  Q+ S L+K GF+  + +
Sbjct: 126 YEESLVVFLEFWRTRKDSPNEYILSSFIQACSGLDGRGRWMVFQLQSFLVKSGFDRDVYV 185

Query: 367 NNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGI 426
              LI  Y K G I  A   F  + E   VTWT++I G    G    +++LF +++   +
Sbjct: 186 GTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMGRSYVSLQLFYQLMEDNV 245

Query: 427 RPDKIAFLGVLSACSHGGFVSMG--LHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQ 486
            PD      VLSACS   F+  G  +H   L   +Y L  D+  +  LID   + G +  
Sbjct: 246 VPDGYILSTVLSACSILPFLEGGKQIHAHIL---RYGLEMDASLMNVLIDSYVKCGRVIA 305

Query: 487 AFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASEG 546
           A  L   MP +     + ++      +    L K AME  +   +   + L  +MYA   
Sbjct: 306 AHKLFNGMPNK----NIISWTTLLSGYKQNALHKEAMELFTSMSK---FGLKPDMYACSS 365

Query: 547 RWSDVARMRKL 553
             +  A +  L
Sbjct: 366 ILTSCASLHAL 366

BLAST of Csa6G005130 vs. Swiss-Prot
Match: PP333_ARATH (Pentatricopeptide repeat-containing protein At4g21300 OS=Arabidopsis thaliana GN=PCMP-E36 PE=3 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 3.6e-90
Identity = 196/598 (32.78%), Postives = 317/598 (53.01%), Query Frame = 1

Query: 22  VLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRN 81
           VL   A    I     LH LVV  G+  E S++N LL +Y KC   D A  LF  M+R +
Sbjct: 245 VLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIKNSLLSMYSKCGRFDDASKLFRMMSRAD 304

Query: 82  VVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVE 141
            V+WN +I G V  G      + + S+  +++ +  G++ PD ITF+ L  S     ++E
Sbjct: 305 TVTWNCMISGYVQSG------LMEESLTFFYEMISSGVL-PDAITFSSLLPSVSKFENLE 364

Query: 142 SGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCCV 201
             +Q+H ++M+    LD F+ SA++D Y KC     A+  FS     D+V++  M+   +
Sbjct: 365 YCKQIHCYIMRHSISLDIFLTSALIDAYFKCRGVSMAQNIFSQCNSVDVVVFTAMISGYL 424

Query: 202 FNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDIL 261
            N L  +++E+FR +       ++ T  S+L       + +LG++LH  +IK+ FD    
Sbjct: 425 HNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILLALKLGRELHGFIIKKGFDNRCN 484

Query: 262 VASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKD 321
           +  +++++Y K   +  A ++F+ +  R+ VSW +MI    Q +    A+ +FR+M    
Sbjct: 485 IGCAVIDMYAKCGRMNLAYEIFERLSKRDIVSWNSMITRCAQSDNPSAAIDIFRQMGVSG 544

Query: 322 YCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAAL 381
            C D ++ ++ LS+C      S    +H  +IK    + +   + LI  Y+KCG + AA+
Sbjct: 545 ICYDCVSISAALSACANLPSESFGKAIHGFMIKHSLASDVYSESTLIDMYAKCGNLKAAM 604

Query: 382 QCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKML-SYGIRPDKIAFLGVLSACSHG 441
             F+ + E ++V+W SII      G  KD++ LF +M+   GIRPD+I FL ++S+C H 
Sbjct: 605 NVFKTMKEKNIVSWNSIIAACGNHGKLKDSLCLFHEMVEKSGIRPDQITFLEIISSCCHV 664

Query: 442 GFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRA 501
           G V  G+ +F  MT  Y + P  EH  C++DL GRAG L +A++ +KSMP          
Sbjct: 665 GDVDEGVRFFRSMTEDYGIQPQQEHYACVVDLFGRAGRLTEAYETVKSMPFPPDAGVWGT 724

Query: 502 FIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDRCE 561
            + ACR H N+ LA+ A       +P     Y L+SN +A+   W  V ++R L+ +R  
Sbjct: 725 LLGACRLHKNVELAEVASSKLMDLDPSNSGYYVLISNAHANAREWESVTKVRSLMKEREV 784

Query: 562 QKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTMKKDYKFTASQVDIVPE 617
           QK PG SW+EI    HLF+SGD +HP+S  +Y++L  LL  ++ +       + + PE
Sbjct: 785 QKIPGYSWIEINKRTHLFVSGDVNHPESSHIYSLLNSLLGELRLEGYIPQPYLPLHPE 835


HSP 2 Score: 209.1 bits (531), Expect = 1.3e-52
Identity = 143/504 (28.37%), Postives = 243/504 (48.21%), Query Frame = 1

Query: 9   FGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLD 68
           FG S  V +F   ++KA   + +      L   V  LG+     V + L++ Y++   +D
Sbjct: 132 FGVSPDVSTFPC-LVKACVALKNFKGIDFLSDTVSSLGMDCNEFVASSLIKAYLEYGKID 191

Query: 69  SARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFN 128
               LFD + +++ V WN ++ G    G          S+   F  M M  + P+ +TF+
Sbjct: 192 VPSKLFDRVLQKDCVIWNVMLNGYAKCG-------ALDSVIKGFSVMRMDQISPNAVTFD 251

Query: 129 GLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYR 188
            +   C     ++ G QLH  V+  G D +  + ++++  Y+KCG ++DA   F  +   
Sbjct: 252 CVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIKNSLLSMYSKCGRFDDASKLFRMMSRA 311

Query: 189 DLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLH 248
           D V WN M+   V + L  E++  F  M   G   D  TFSSLL S     + E  KQ+H
Sbjct: 312 DTVTWNCMISGYVQSGLMEESLTFFYEMISSGVLPDAITFSSLLPSVSKFENLEYCKQIH 371

Query: 249 CLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGK 308
           C +++ S  LDI + S+L++ Y K   +  A+ +F +  + + V +T MI GY  +    
Sbjct: 372 CYIMRHSISLDIFLTSALIDAYFKCRGVSMAQNIFSQCNSVDVVVFTAMISGYLHNGLYI 431

Query: 309 EAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLI 368
           +++++FR + +    P+E+T  S+L   G         ++H  +IK GF+   +I   +I
Sbjct: 432 DSLEMFRWLVKVKISPNEITLVSILPVIGILLALKLGRELHGFIIKKGFDNRCNIGCAVI 491

Query: 369 YAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKI 428
             Y+KCG +  A + F  +++ D+V+W S+I   A       A+ +F +M   GI  D +
Sbjct: 492 DMYAKCGRMNLAYEIFERLSKRDIVSWNSMITRCAQSDNPSAAIDIFRQMGVSGICYDCV 551

Query: 429 AFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKS 488
           +    LSAC++    S G      M  ++ L  D    + LID+  + G+L  A ++ K+
Sbjct: 552 SISAALSACANLPSESFGKAIHGFMI-KHSLASDVYSESTLIDMYAKCGNLKAAMNVFKT 611

Query: 489 MPKEAGPDALRAFIRACRTHGNLR 513
           M KE    +  + I AC  HG L+
Sbjct: 612 M-KEKNIVSWNSIIAACGNHGKLK 625


HSP 3 Score: 132.5 bits (332), Expect = 1.6e-29
Identity = 90/315 (28.57%), Postives = 146/315 (46.35%), Query Frame = 1

Query: 130 LFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDA-RLAFSCILYR 189
           L ++C   N +  G+Q+H+F++      D +    ++  YA CG + D  ++ +   L R
Sbjct: 41  LLQACSNPNLLRQGKQVHAFLIVNSISGDSYTDERILGMYAMCGSFSDCGKMFYRLDLRR 100

Query: 190 DLVL-WNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSC----KYKGSGEL 249
             +  WN ++   V N L  +A+  +  M   G   D  TF  L+ +C     +KG   L
Sbjct: 101 SSIRPWNSIISSFVRNGLLNQALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKGIDFL 160

Query: 250 GKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQ 309
              +  L      D +  VASSL+  Y +   +    K+FD +  ++ V W  M+ GY +
Sbjct: 161 SDTVSSL----GMDCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQKDCVIWNVMLNGYAK 220

Query: 310 HEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSI 369
                  +K F  M      P+ +TF  VLS C         +Q+H  ++  G +   SI
Sbjct: 221 CGALDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSI 280

Query: 370 NNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGI 429
            N L+  YSKCG    A + FR+++  D VTW  +I G    GL ++++  F +M+S G+
Sbjct: 281 KNSLLSMYSKCGRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFYEMISSGV 340

Query: 430 RPDKIAFLGVLSACS 439
            PD I F  +L + S
Sbjct: 341 LPDAITFSSLLPSVS 351

BLAST of Csa6G005130 vs. TrEMBL
Match: A0A0A0K863_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G005130 PE=4 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 616/616 (100.00%), Postives = 616/616 (100.00%), Query Frame = 1

Query: 1   MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRV 60
           MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRV
Sbjct: 1   MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRV 60

Query: 61  YVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLV 120
           YVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLV
Sbjct: 61  YVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLV 120

Query: 121 DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARL 180
           DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARL
Sbjct: 121 DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARL 180

Query: 181 AFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGS 240
           AFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGS
Sbjct: 181 AFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGS 240

Query: 241 GELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVG 300
           GELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVG
Sbjct: 241 GELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVG 300

Query: 301 YGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF 360
           YGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF
Sbjct: 301 YGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF 360

Query: 361 LSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLS 420
           LSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLS
Sbjct: 361 LSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLS 420

Query: 421 YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLD 480
           YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLD
Sbjct: 421 YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLD 480

Query: 481 QAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASE 540
           QAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASE
Sbjct: 481 QAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASE 540

Query: 541 GRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTM 600
           GRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTM
Sbjct: 541 GRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTM 600

Query: 601 KKDYKFTASQVDIVPE 617
           KKDYKFTASQVDIVPE
Sbjct: 601 KKDYKFTASQVDIVPE 616

BLAST of Csa6G005130 vs. TrEMBL
Match: A0A067HEI7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g044628mg PE=4 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 4.2e-186
Identity = 333/590 (56.44%), Postives = 422/590 (71.53%), Query Frame = 1

Query: 16  HSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFD 75
           HSF    LK +A    +     LHS ++KLGL N+LS+QN++L VYVKC+  D    LFD
Sbjct: 63  HSFYSQALKVSAKFGFLQQGKQLHSHIMKLGLCNKLSLQNQVLHVYVKCKAFDDMEKLFD 122

Query: 76  EMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCV 135
           EM  RN+V+WNT+I G+++ G          ++  YF++ML+  V  D ITFN L R+CV
Sbjct: 123 EMRVRNIVTWNTLISGIINCG---------GNVTPYFRRMLLDNVRLDHITFNSLLRACV 182

Query: 136 VLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNV 195
             +D+E GR+LHSF++K+GF L+CFV SA+VD Y KCG  EDAR  F  +L RDLVLWNV
Sbjct: 183 QADDIEVGRRLHSFILKVGFGLNCFVSSALVDLYGKCGFVEDARRVFDEVLCRDLVLWNV 242

Query: 196 MLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQS 255
           M+ C   N L   AI VF LM+LEG KGD FTFSSL++SC   GS +LG+Q+H L+IKQS
Sbjct: 243 MVSCYALNCLGDGAIAVFNLMRLEGMKGDYFTFSSLVNSCGTLGSSKLGRQIHGLVIKQS 302

Query: 256 FDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFR 315
           FDLD+LVA+SLV++Y KN N+ DA +VFD M  +N VSW TM+VG+GQ+  G+EAVKL R
Sbjct: 303 FDLDVLVATSLVDMYAKNGNIDDACRVFDGMTAKNVVSWNTMVVGFGQNGDGREAVKLLR 362

Query: 316 RMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCG 375
            M +  +CPDE+T AS+LSSCG  S + E  QVH+  IK G +AFLSI N LI AYSKCG
Sbjct: 363 DMLQGSFCPDEVTLASILSSCGSLSISCETRQVHAYAIKNGVQAFLSIENALINAYSKCG 422

Query: 376 IIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLS 435
            IA ALQCF  + EPDLVTWTSII   A  GL K+++++F+KMLS+ +RPD IAFL VLS
Sbjct: 423 SIAGALQCFGSVKEPDLVTWTSIIGAYAFHGLSKESIEVFEKMLSHAVRPDSIAFLEVLS 482

Query: 436 ACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGP 495
           ACSHGG VS GL YFNLM + Y ++PDSEH TCL DLLGR G L +A+DLL SMP E   
Sbjct: 483 ACSHGGLVSEGLRYFNLMISDYHILPDSEHYTCLTDLLGRVGLLVEAYDLLASMPIEPRS 542

Query: 496 DALRAFIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKLI 555
           D L AFI AC+ HG++ LAK A E     EP +PVNY+LVSN+YASE  W DVAR+RK++
Sbjct: 543 DTLGAFIGACKVHGSIGLAKWAAEKLLELEPSKPVNYALVSNVYASERCWFDVARLRKMM 602

Query: 556 NDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTMKKD 604
            D C+ K PG SW+EIAG  H F+S DRSHPQ++ +YAML  +   M+++
Sbjct: 603 RDNCDHKVPGCSWIEIAGEIHTFVSSDRSHPQAVHMYAMLCTVFGLMEEN 643

BLAST of Csa6G005130 vs. TrEMBL
Match: F6I669_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0046g01830 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.7e-184
Identity = 327/590 (55.42%), Postives = 436/590 (73.90%), Query Frame = 1

Query: 16  HSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFD 75
           HSFS + LK +A +  +     LH+ V+KLG  N LS+QN++L VYVKC++ +    +FD
Sbjct: 73  HSFSSHALKISAKLGFLHGGKQLHAHVIKLGNCNLLSLQNQVLHVYVKCKEFNDVCKMFD 132

Query: 76  EMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCV 135
           EM  +NVVSWNT+ICG+V+G    +F + +   F YF++M++ ++ P+ IT NGL R+ +
Sbjct: 133 EMPLKNVVSWNTLICGVVEGNC--KFALVRLG-FHYFRQMVLEMMAPNCITLNGLLRASI 192

Query: 136 VLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNV 195
            LNDV   RQLH F++K GFD +CFVGSA+VD YAK GL ++A+ AF  +  RDLVLWNV
Sbjct: 193 ELNDVGICRQLHCFILKSGFDSNCFVGSALVDSYAKFGLVDEAQSAFDEVSSRDLVLWNV 252

Query: 196 MLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQS 255
           M+ C   N +  +A  VF+LM+LEG KGD+FTF+S+++SC   GS  LGKQ+H L+I+ S
Sbjct: 253 MVSCYALNGVQGKAFGVFKLMRLEGVKGDNFTFTSMINSCGVLGSCGLGKQVHGLIIRLS 312

Query: 256 FDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFR 315
           FDLD+LVAS+LV++Y+KN+N+ DARK FD M  +N VSWTTMIVGYGQH  GKEA++L +
Sbjct: 313 FDLDVLVASALVDMYSKNENIEDARKAFDGMIVKNIVSWTTMIVGYGQHGDGKEAMRLLQ 372

Query: 316 RMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCG 375
            M R    PDEL  AS+LSSCG  S  SE++QVH+ +++ GFEAFLSI N L+ AYSKCG
Sbjct: 373 EMIRVYTYPDELALASILSSCGNLSATSEVVQVHAYVVENGFEAFLSIANALVSAYSKCG 432

Query: 376 IIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLS 435
            I +A Q F  +AEPD+++WTS++   A  GL K+ V++F+KML   +RPDK+AFLGVLS
Sbjct: 433 SIGSAFQSFSSVAEPDIISWTSLMGAYAFHGLSKEGVEVFEKMLFSNVRPDKVAFLGVLS 492

Query: 436 ACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGP 495
           AC+HGGFV  GLHYFNLM N YQ++PDSEH TC+IDLLGRAG LD+A +LL SMP E   
Sbjct: 493 ACAHGGFVLEGLHYFNLMINVYQIMPDSEHYTCIIDLLGRAGFLDEAINLLTSMPVEPRS 552

Query: 496 DALRAFIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKLI 555
           D L AF+ AC+ H N+ LA+ A E  F  EP+EP NYSL+SNMYAS G W DVAR+RKL+
Sbjct: 553 DTLGAFLGACKVHRNVGLARWASEKLFVMEPNEPANYSLMSNMYASVGHWFDVARVRKLM 612

Query: 556 NDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTMKKD 604
            +RC+ K PG SW+EIAG  H F+S D++HP+++ +Y ML LL+  M++D
Sbjct: 613 RERCDFKVPGCSWMEIAGEVHTFVSRDKTHPRAVQVYGMLDLLVRLMEED 659

BLAST of Csa6G005130 vs. TrEMBL
Match: W9T0A0_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_021461 PE=4 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 3.0e-184
Identity = 323/593 (54.47%), Postives = 414/593 (69.81%), Query Frame = 1

Query: 6   STHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCR 65
           S HF  S L HS     LK +A +  +     LH  ++K GL  ++ +QN++L  YV+C+
Sbjct: 63  SAHFHNSHLAHSLCSKALKISAKMGFLSEGKQLHGHLLKFGLYIDMFLQNQILNFYVRCK 122

Query: 66  DLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGI 125
           +L     +  EM  RNVV+WN VICG+VD      FK      F YFK+ML   V PD I
Sbjct: 123 ELSDGHKVLGEMTVRNVVAWNAVICGVVDDR--SYFKSSFSLGFSYFKRMLRETVHPDEI 182

Query: 126 TFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCI 185
           T N L R+C+ LNDVE   QLH FV+K+GF  +CFV +AVV+ Y KCGL EDAR AF  +
Sbjct: 183 TLNVLLRACIALNDVEVALQLHCFVLKLGFASNCFVSAAVVELYEKCGLVEDARCAFDIV 242

Query: 186 LYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGK 245
           +YRDLVLWNVM+YC   N L  EAI VF  M+LEG +GDDFTF SLLS C   GS ELGK
Sbjct: 243 VYRDLVLWNVMVYCYASNCLVWEAIRVFEFMRLEGVEGDDFTFCSLLSLCGSSGSYELGK 302

Query: 246 QLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHE 305
           Q+H ++IK SFD+D++VASSL+++Y+KN+N+  A KVFD M  +N VSW T+IVGYGQH 
Sbjct: 303 QIHGIIIKHSFDIDVIVASSLIDMYSKNENIGAAHKVFDMMSIKNVVSWNTIIVGYGQHG 362

Query: 306 YGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINN 365
            GK A+KLF +M R  + PD LT ASV+SSCG    +S LMQVH+C IK GF++FLS  N
Sbjct: 363 EGKGAIKLFGKMLRCGFSPDNLTMASVVSSCGKVGLSSALMQVHACAIKFGFKSFLSTVN 422

Query: 366 GLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRP 425
            LI AYSKCG I +A QCF L+ EPDL TWTSIIC  A  GL + A++ F+KML+ GI+P
Sbjct: 423 ALINAYSKCGSIVSAFQCFSLVTEPDLFTWTSIICAYAFHGLAEGAIEYFEKMLTCGIKP 482

Query: 426 DKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDL 485
           D+I FLGVLSACSH G +  GL YF +MT  YQ++P+ EH T LIDL+GRAG L++AF +
Sbjct: 483 DRITFLGVLSACSHRGLIDKGLRYFEMMTKNYQILPEPEHYTSLIDLVGRAGLLEEAFTI 542

Query: 486 LKSMPKEAGPDALRAFIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRW 545
           L ++P EAGP+   AF+ AC+ HG+LRL + A E  F  EP   VNY+++SN Y+ EGRW
Sbjct: 543 LSAIPTEAGPNTFGAFLGACKRHGDLRLTEWAAEKLFTLEPSVAVNYTIMSNAYSHEGRW 602

Query: 546 SDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLL 597
            DVAR+RK++   C  K+PG SWVEI G  H F+S D+SHP+++++Y MLGLL
Sbjct: 603 LDVARIRKMMRSNCSLKSPGCSWVEICGDVHTFVSSDQSHPKAVEVYDMLGLL 653

BLAST of Csa6G005130 vs. TrEMBL
Match: A5BLE5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010801 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 1.1e-178
Identity = 320/590 (54.24%), Postives = 428/590 (72.54%), Query Frame = 1

Query: 16  HSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFD 75
           HSFS + LK +A +  +     LH+ V+KLG  N LS+QN++L VYVKC++ +    +FD
Sbjct: 73  HSFSSHALKISAKLGFLHGGKQLHAHVIKLGXCNLLSLQNQVLHVYVKCKEFNDVCKMFD 132

Query: 76  EMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCV 135
           EM  +NVVSWNT+ICG+V+G    +F + +   F  F++M++ ++ P+ IT NGL R+ +
Sbjct: 133 EMPLKNVVSWNTLICGVVEGNC--KFALVRLG-FHCFRQMVLEMMAPNCITLNGLLRASI 192

Query: 136 VLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNV 195
            LNDV   RQLH F++K GFD +CFVGSA+VD YAK GL ++A+ AF  +  RDLVLWNV
Sbjct: 193 ELNDVGICRQLHCFILKSGFDSNCFVGSALVDSYAKFGLVDEAQSAFDEVSSRDLVLWNV 252

Query: 196 MLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQS 255
           M+ C   N +  +A  VF+LM+LEG KGD FTF+S+++SC   GS  LGKQ+H L+I+ S
Sbjct: 253 MVSCYALNGVQGKAFGVFKLMRLEGVKGDXFTFTSMINSCGVLGSCGLGKQVHGLIIRLS 312

Query: 256 FDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFR 315
           FDLD+LVAS+LV++Y+KN+N+ DARK FD M  +N VSWTTM VGYGQH  GKE ++L +
Sbjct: 313 FDLDVLVASALVDMYSKNENIEDARKAFDGMXVKNIVSWTTMXVGYGQHGDGKEXMRLLQ 372

Query: 316 RMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCG 375
            M R    PDEL  AS+LSSCG  S  SE++QVH+ +++ GFEAFLSI N L+ AYSKCG
Sbjct: 373 EMIRVYTYPDELALASILSSCGNLSATSEVVQVHAYVVENGFEAFLSIANALVSAYSKCG 432

Query: 376 IIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLS 435
            I +A Q F  +AEPD+++WTS++   A  GL K  V +F+K+LS  +RPDK+AFLGVLS
Sbjct: 433 SIGSAFQSFSSVAEPDIISWTSLMGAYAFHGLSKQGVDVFEKILSSNVRPDKVAFLGVLS 492

Query: 436 ACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGP 495
           AC+HGGFV  GLHYFNLM N YQ++PDSEH T +IDLLGRAG LD+A +LL SMP E   
Sbjct: 493 ACAHGGFVLEGLHYFNLMINVYQIMPDSEHYTSIIDLLGRAGFLDEAVNLLTSMPVEPRS 552

Query: 496 DALRAFIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKLI 555
           D L AF+ AC+ + N+ LA+ A E  F  EP+EP  YSL+SNMYAS G W DVAR+RKL+
Sbjct: 553 DTLGAFLGACKVYRNVGLARWASEKLFVMEPNEPGKYSLMSNMYASVGHWFDVARVRKLM 612

Query: 556 NDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTMKKD 604
            +RC+ K PG SW+E AG  H F+S D++HP+++ +Y ML LL+  MK++
Sbjct: 613 RERCDFKVPGCSWMETAGEVHTFVSRDKTHPRAVQVYGMLDLLVRLMKEE 659

BLAST of Csa6G005130 vs. TAIR10
Match: AT2G46050.1 (AT2G46050.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 454.9 bits (1169), Expect = 7.9e-128
Identity = 250/554 (45.13%), Postives = 342/554 (61.73%), Query Frame = 1

Query: 21  NVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARR 80
           +V K +A ++ +      H  +VK G+ N L +QNKLL+ Y K R+ D A  LFDEM  R
Sbjct: 41  SVSKLSASLDHLSDVKQEHGFMVKQGIYNSLFLQNKLLQAYTKIREFDDADKLFDEMPLR 100

Query: 81  NVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDV 140
           N+V+WN +I G++     G+   R H  F Y  ++L   V  D ++F GL R C    ++
Sbjct: 101 NIVTWNILIHGVIQRD--GDTNHRAHLGFCYLSRILFTDVSLDHVSFMGLIRLCTDSTNM 160

Query: 141 ESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCC 200
           ++G QLH  ++K G +  CF  +++V FY KCGL  +AR  F  +L RDLVLWN ++   
Sbjct: 161 KAGIQLHCLMVKQGLESSCFPSTSLVHFYGKCGLIVEARRVFEAVLDRDLVLWNALVSSY 220

Query: 201 VFNSLSREAIEVFRLMQLEG--FKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDL 260
           V N +  EA  + +LM  +   F+GD FTFSSLLS+C+     E GKQ+H +L K S+  
Sbjct: 221 VLNGMIDEAFGLLKLMGSDKNRFRGDYFTFSSLLSACRI----EQGKQIHAILFKVSYQF 280

Query: 261 DILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMF 320
           DI VA++L+N+Y K+++L DAR+ F+ M  RN VSW  MIVG+ Q+  G+EA++LF +M 
Sbjct: 281 DIPVATALLNMYAKSNHLSDARECFESMVVRNVVSWNAMIVGFAQNGEGREAMRLFGQML 340

Query: 321 RKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIA 380
            ++  PDELTFASVLSSC   S   E+ QV + + K G   FLS+ N LI +YS+ G ++
Sbjct: 341 LENLQPDELTFASVLSSCAKFSAIWEIKQVQAMVTKKGSADFLSVANSLISSYSRNGNLS 400

Query: 381 AALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACS 440
            AL CF  I EPDLV+WTS+I  LA  G  ++++++F+ ML   ++PDKI FL VLSACS
Sbjct: 401 EALLCFHSIREPDLVSWTSVIGALASHGFAEESLQMFESMLQ-KLQPDKITFLEVLSACS 460

Query: 441 HGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDAL 500
           HGG V  GL  F  MT  Y++  + EH TCLIDLLGRAG +D+A D+L SMP E    AL
Sbjct: 461 HGGLVQEGLRCFKRMTEFYKIEAEDEHYTCLIDLLGRAGFIDEASDVLNSMPTEPSTHAL 520

Query: 501 RAFIRACRTHGNLRL----AKRAMEFASEPDEPVNYSLVSNMYASEGRWSDVARMRKLIN 560
            AF   C  H         AK+ +E   EP +PVNYS++SN Y SEG W+  A +RK   
Sbjct: 521 AAFTGGCNIHEKRESMKWGAKKLLEI--EPTKPVNYSILSNAYVSEGHWNQAALLRKRER 580

Query: 561 DRC-EQKTPGLSWV 568
             C   KTPG SW+
Sbjct: 581 RNCYNPKTPGCSWL 585

BLAST of Csa6G005130 vs. TAIR10
Match: AT2G13600.1 (AT2G13600.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 369.0 bits (946), Expect = 5.7e-102
Identity = 207/634 (32.65%), Postives = 331/634 (52.21%), Query Frame = 1

Query: 38  LHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGY 97
           +H+ V+K G  NE+ +QN+L+  Y KC  L+  R +FD+M +RN+ +WN+V+ GL   G+
Sbjct: 42  VHASVIKSGFSNEIFIQNRLIDAYSKCGSLEDGRQVFDKMPQRNIYTWNSVVTGLTKLGF 101

Query: 98  GGE----FKMRQHSIFLYFKKMLMGLVDPDGI--------------------TFNGLFRS 157
             E    F+         +  M+ G    D                      +F  +  +
Sbjct: 102 LDEADSLFRSMPERDQCTWNSMVSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSA 161

Query: 158 CVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLW 217
           C  LND+  G Q+HS + K  F  D ++GSA+VD Y+KCG   DA+  F  +  R++V W
Sbjct: 162 CSGLNDMNKGVQVHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQRVFDEMGDRNVVSW 221

Query: 218 NVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIK 277
           N ++ C   N  + EA++VF++M     + D+ T +S++S+C    + ++G+++H  ++K
Sbjct: 222 NSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVK 281

Query: 278 QS-FDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNS--------------------- 337
                 DI+++++ V++Y K   + +AR +FD MP RN                      
Sbjct: 282 NDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAASTKAARL 341

Query: 338 ----------VSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSG 397
                     VSW  +I GY Q+   +EA+ LF  + R+  CP   +FA++L +C   + 
Sbjct: 342 MFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKACADLAE 401

Query: 398 ASELMQVHSCLIKLGF------EAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTW 457
               MQ H  ++K GF      E  + + N LI  Y KCG +      FR + E D V+W
Sbjct: 402 LHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMMERDCVSW 461

Query: 458 TSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTN 517
            ++I G A  G   +A++LF +ML  G +PD I  +GVLSAC H GFV  G HYF+ MT 
Sbjct: 462 NAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSACGHAGFVEEGRHYFSSMTR 521

Query: 518 QYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAK 577
            + + P  +H TC++DLLGRAG L++A  +++ MP +       + + AC+ H N+ L K
Sbjct: 522 DFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSVIWGSLLAACKVHRNITLGK 581

Query: 578 RAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDRCEQKTPGLSWVEIAGYN 608
              E     EP     Y L+SNMYA  G+W DV  +RK +      K PG SW++I G++
Sbjct: 582 YVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSMRKEGVTKQPGCSWIKIQGHD 641


HSP 2 Score: 120.9 bits (302), Expect = 2.7e-27
Identity = 82/313 (26.20%), Postives = 152/313 (48.56%), Query Frame = 1

Query: 179 RLAFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLE-GFKGDDFTFSSLLSSCKY 238
           +LA     + D   +  +L  C+ + LS   +       ++ GF  + F  + L+ +   
Sbjct: 8   KLAADLSSFTDSSPFAKLLDSCIKSKLSAIYVRYVHASVIKSGFSNEIFIQNRLIDAYSK 67

Query: 239 KGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTM 298
            GS E G+Q+   + +++    I   +S+V   TK   L +A  +F  MP R+  +W +M
Sbjct: 68  CGSLEDGRQVFDKMPQRN----IYTWNSVVTGLTKLGFLDEADSLFRSMPERDQCTWNSM 127

Query: 299 IVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGF 358
           + G+ QH+  +EA+  F  M ++ +  +E +FASVLS+C   +  ++ +QVHS + K  F
Sbjct: 128 VSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSACSGLNDMNKGVQVHSLIAKSPF 187

Query: 359 EAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDK 418
            + + I + L+  YSKCG +  A + F  + + ++V+W S+I      G   +A+ +F  
Sbjct: 188 LSDVYIGSALVDMYSKCGNVNDAQRVFDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQM 247

Query: 419 MLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAG 478
           ML   + PD++    V+SAC+    + +G      +    +L  D       +D+  +  
Sbjct: 248 MLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCS 307

Query: 479 SLDQAFDLLKSMP 491
            + +A  +  SMP
Sbjct: 308 RIKEARFIFDSMP 316


HSP 3 Score: 42.4 bits (98), Expect = 1.2e-03
Identity = 24/72 (33.33%), Postives = 40/72 (55.56%), Query Frame = 1

Query: 21  NVLKAAAPVNSIPHDTLLHSLVVKLG-LVNELSVQNKLLRVYVKCRDLDSARNLFDEMAR 80
           +V+ A A +++I     +H  VVK   L N++ + N  + +Y KC  +  AR +FD M  
Sbjct: 258 SVISACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPI 317

Query: 81  RNVVSWNTVICG 92
           RNV++  ++I G
Sbjct: 318 RNVIAETSMISG 329

BLAST of Csa6G005130 vs. TAIR10
Match: AT4G18750.1 (AT4G18750.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 344.4 bits (882), Expect = 1.5e-94
Identity = 192/581 (33.05%), Postives = 325/581 (55.94%), Query Frame = 1

Query: 17  SFSFN-VLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFD 76
           S++F+ V K+ + + S+     LH  ++K G     SV N L+  Y+K + +DSAR +FD
Sbjct: 195 SYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFD 254

Query: 77  EMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCV 136
           EM  R+V+SWN++I G V  G       +  S+F+   +ML+  ++ D  T   +F  C 
Sbjct: 255 EMTERDVISWNSIINGYVSNGLAE----KGLSVFV---QMLVSGIEIDLATIVSVFAGCA 314

Query: 137 VLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNV 196
               +  GR +HS  +K  F  +    + ++D Y+KCG  + A+  F  +  R +V +  
Sbjct: 315 DSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTS 374

Query: 197 MLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQS 256
           M+       L+ EA+++F  M+ EG   D +T +++L+ C      + GK++H  + +  
Sbjct: 375 MIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKEND 434

Query: 257 FDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFR 316
              DI V+++L+++Y K  ++ +A  VF EM  ++ +SW T+I GY ++ Y  EA+ LF 
Sbjct: 435 LGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFN 494

Query: 317 RMFR-KDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKC 376
            +   K + PDE T A VL +C   S   +  ++H  +++ G+ +   + N L+  Y+KC
Sbjct: 495 LLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKC 554

Query: 377 GIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVL 436
           G +  A   F  IA  DLV+WT +I G  + G  K+A+ LF++M   GI  D+I+F+ +L
Sbjct: 555 GALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLL 614

Query: 437 SACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAG 496
            ACSH G V  G  +FN+M ++ ++ P  EH  C++D+L R G L +A+  +++MP    
Sbjct: 615 YACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPD 674

Query: 497 PDALRAFIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKL 556
                A +  CR H +++LA++  E  F  EP+    Y L++N+YA   +W  V R+RK 
Sbjct: 675 ATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKR 734

Query: 557 INDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAML 594
           I  R  +K PG SW+EI G  ++F++GD S+P++ ++ A L
Sbjct: 735 IGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFL 768


HSP 2 Score: 194.1 bits (492), Expect = 2.5e-49
Identity = 140/465 (30.11%), Postives = 227/465 (48.82%), Query Frame = 1

Query: 21  NVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARR 80
           +VL+  A   S+     + + +   G V + ++ +KL  +Y  C DL  A  +FDE+   
Sbjct: 99  SVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIE 158

Query: 81  NVVSWNTVICGLVDGG-YGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLND 140
             + WN ++  L   G + G   +        FKKM+   V+ D  TF+ + +S   L  
Sbjct: 159 KALFWNILMNELAKSGDFSGSIGL--------FKKMMSSGVEMDSYTFSCVSKSFSSLRS 218

Query: 141 VESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYC 200
           V  G QLH F++K GF     VG+++V FY K    + AR  F  +  RD++ WN ++  
Sbjct: 219 VHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIING 278

Query: 201 CVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLD 260
            V N L+ + + VF  M + G + D  T  S+ + C       LG+ +H + +K  F  +
Sbjct: 279 YVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSRE 338

Query: 261 ILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFR 320
               ++L+++Y+K  +L  A+ VF EM  R+ VS+T+MI GY +     EAVKLF  M  
Sbjct: 339 DRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEE 398

Query: 321 KDYCPDELTFASVLSSCGFTSGASELMQVHSCLIK--LGFEAFLSINNGLIYAYSKCGII 380
           +   PD  T  +VL+ C       E  +VH  + +  LGF+ F+S  N L+  Y+KCG +
Sbjct: 399 EGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVS--NALMDMYAKCGSM 458

Query: 381 AAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKML-SYGIRPDKIAFLGVLSA 440
             A   F  +   D+++W +II G +      +A+ LF+ +L      PD+     VL A
Sbjct: 459 QEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPA 518

Query: 441 CSHGGFVSMG--LHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSL 480
           C+       G  +H + +M N Y    D      L+D+  + G+L
Sbjct: 519 CASLSAFDKGREIHGY-IMRNGY--FSDRHVANSLVDMYAKCGAL 550


HSP 3 Score: 95.5 bits (236), Expect = 1.2e-19
Identity = 73/303 (24.09%), Postives = 131/303 (43.23%), Query Frame = 1

Query: 224 DDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVF 283
           D  T  S+L  C    S + GK++   +    F +D  + S L  +YT   +L +A +VF
Sbjct: 93  DPRTLCSVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVF 152

Query: 284 DEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGAS 343
           DE+    ++ W  ++    +      ++ LF++M       D  TF+ V  S        
Sbjct: 153 DEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVH 212

Query: 344 ELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLA 403
              Q+H  ++K GF    S+ N L+  Y K   + +A + F  + E D+++W SII G  
Sbjct: 213 GGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYV 272

Query: 404 LCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDS 463
             GL +  + +F +ML  GI  D    + V + C+    +S+G    ++         + 
Sbjct: 273 SNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKAC-FSRED 332

Query: 464 EHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASE 523
                L+D+  + G LD A    K++ +E    ++ ++      +    LA  A++   E
Sbjct: 333 RFCNTLLDMYSKCGDLDSA----KAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEE 390

Query: 524 PDE 527
            +E
Sbjct: 393 MEE 390

BLAST of Csa6G005130 vs. TAIR10
Match: AT4G39530.1 (AT4G39530.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 335.1 bits (858), Expect = 9.1e-92
Identity = 197/581 (33.91%), Postives = 316/581 (54.39%), Query Frame = 1

Query: 22  VLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRN 81
           VL A + +  +     +H+ +++ GL  + S+ N L+  YVKC  + +A  LF+ M  +N
Sbjct: 255 VLSACSILPFLEGGKQIHAHILRYGLEMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKN 314

Query: 82  VVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVE 141
           ++SW T++ G           + + ++ L+      GL  PD    + +  SC  L+ + 
Sbjct: 315 IISWTTLLSGYKQNA------LHKEAMELFTSMSKFGL-KPDMYACSSILTSCASLHALG 374

Query: 142 SGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVML--YC 201
            G Q+H++ +K     D +V ++++D YAKC    DAR  F      D+VL+N M+  Y 
Sbjct: 375 FGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCLTDARKVFDIFAAADVVLFNAMIEGYS 434

Query: 202 CVFNSLS-REAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDL 261
            +       EA+ +FR M+    +    TF SLL +     S  L KQ+H L+ K   +L
Sbjct: 435 RLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLLRASASLTSLGLSKQIHGLMFKYGLNL 494

Query: 262 DILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMF 321
           DI   S+L++VY+    L D+R VFDEM  ++ V W +M  GY Q    +EA+ LF  + 
Sbjct: 495 DIFAGSALIDVYSNCYCLKDSRLVFDEMKVKDLVIWNSMFAGYVQQSENEEALNLFLELQ 554

Query: 322 RKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIA 381
                PDE TFA+++++ G  +      + H  L+K G E    I N L+  Y+KCG   
Sbjct: 555 LSRERPDEFTFANMVTAAGNLASVQLGQEFHCQLLKRGLECNPYITNALLDMYAKCGSPE 614

Query: 382 AALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACS 441
            A + F   A  D+V W S+I   A  G  K A+++ +KM+S GI P+ I F+GVLSACS
Sbjct: 615 DAHKAFDSAASRDVVCWNSVISSYANHGEGKKALQMLEKMMSEGIEPNYITFVGVLSACS 674

Query: 442 HGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDAL 501
           H G V  GL  F LM  ++ + P++EH  C++ LLGRAG L++A +L++ MP +      
Sbjct: 675 HAGLVEDGLKQFELML-RFGIEPETEHYVCMVSLLGRAGRLNKARELIEKMPTKPAAIVW 734

Query: 502 RAFIRACRTHGNLRLAKRAMEFA--SEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDR 561
           R+ +  C   GN+ LA+ A E A  S+P +  +++++SN+YAS+G W++  ++R+ +   
Sbjct: 735 RSLLSGCAKAGNVELAEHAAEMAILSDPKDSGSFTMLSNIYASKGMWTEAKKVRERMKVE 794

Query: 562 CEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLL 598
              K PG SW+ I    H+F+S D+SH ++  +Y +L  LL
Sbjct: 795 GVVKEPGRSWIGINKEVHIFLSKDKSHCKANQIYEVLDDLL 827


HSP 2 Score: 197.2 bits (500), Expect = 3.0e-50
Identity = 143/499 (28.66%), Postives = 237/499 (47.49%), Query Frame = 1

Query: 38  LHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGY 97
           L S +VK G   ++ V   L+  Y+K  ++D AR +FD +  ++ V+W T+I G V  G 
Sbjct: 170 LQSFLVKSGFDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMG- 229

Query: 98  GGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDL 157
                 R +     F +++   V PDG   + +  +C +L  +E G+Q+H+ +++ G ++
Sbjct: 230 ------RSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLEM 289

Query: 158 DCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQ 217
           D  + + ++D Y KCG    A   F+ +  ++++ W  +L     N+L +EA+E+F  M 
Sbjct: 290 DASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSMS 349

Query: 218 LEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLY 277
             G K D +  SS+L+SC    +   G Q+H   IK +   D  V +SL+++Y K D L 
Sbjct: 350 KFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCLT 409

Query: 278 DARKVFDEMPTRNSVSWTTMIVGY---GQHEYGKEAVKLFRRMFRKDYCPDELTFASVLS 337
           DARKVFD     + V +  MI GY   G      EA+ +FR M  +   P  LTF S+L 
Sbjct: 410 DARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLLR 469

Query: 338 SCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVT 397
           +    +      Q+H  + K G    +   + LI  YS C  +  +   F  +   DLV 
Sbjct: 470 ASASLTSLGLSKQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKDSRLVFDEMKVKDLVI 529

Query: 398 WTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMT 457
           W S+  G       ++A+ LF ++     RPD+  F  +++A  +   V +G   F+   
Sbjct: 530 WNSMFAGYVQQSENEEALNLFLELQLSRERPDEFTFANMVTAAGNLASVQLG-QEFHCQL 589

Query: 458 NQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDAL--RAFIRACRTHGNLR 517
            +  L  +      L+D+  + GS + A    K+    A  D +   + I +   HG  +
Sbjct: 590 LKRGLECNPYITNALLDMYAKCGSPEDAH---KAFDSAASRDVVCWNSVISSYANHGEGK 649

Query: 518 LAKRAME-FASEPDEPVNY 531
            A + +E   SE  EP NY
Sbjct: 650 KALQMLEKMMSEGIEP-NY 656


HSP 3 Score: 191.4 bits (485), Expect = 1.6e-48
Identity = 122/433 (28.18%), Postives = 217/433 (50.12%), Query Frame = 1

Query: 22  VLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRN 81
           +L+  A  + + +  ++H  ++  GL  +  + N L+ +Y +   +  AR +F++M  RN
Sbjct: 50  LLQLRASDDLLHYQNVVHGQIIVWGLELDTYLSNILINLYSRAGGMVYARKVFEKMPERN 109

Query: 82  VVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVE 141
           +VSW+T++      G      + + S+ ++ +        P+    +   ++C  L+   
Sbjct: 110 LVSWSTMVSACNHHG------IYEESLVVFLEFWRTRKDSPNEYILSSFIQACSGLDG-- 169

Query: 142 SGR----QLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVML 201
            GR    QL SF++K GFD D +VG+ ++DFY K G  + ARL F  +  +  V W  M+
Sbjct: 170 RGRWMVFQLQSFLVKSGFDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMI 229

Query: 202 YCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFD 261
             CV    S  ++++F  +  +    D +  S++LS+C      E GKQ+H  +++   +
Sbjct: 230 SGCVKMGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLE 289

Query: 262 LDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRM 321
           +D  + + L++ Y K   +  A K+F+ MP +N +SWTT++ GY Q+   KEA++LF  M
Sbjct: 290 MDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSM 349

Query: 322 FRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGII 381
            +    PD    +S+L+SC          QVH+  IK        + N LI  Y+KC  +
Sbjct: 350 SKFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCL 409

Query: 382 AAALQCFRLIAEPDLVTWTSIICGLALCGLE---KDAVKLFDKMLSYGIRPDKIAFLGVL 441
             A + F + A  D+V + ++I G +  G +    +A+ +F  M    IRP  + F+ +L
Sbjct: 410 TDARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLL 469

Query: 442 SACSHGGFVSMGL 448
            A +     S+GL
Sbjct: 470 RASA--SLTSLGL 472


HSP 4 Score: 162.2 bits (409), Expect = 1.1e-39
Identity = 97/342 (28.36%), Postives = 177/342 (51.75%), Query Frame = 1

Query: 146 LHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCCVFNSL 205
           +H  ++  G +LD ++ + +++ Y++ G    AR  F  +  R+LV W+ M+  C  + +
Sbjct: 66  VHGQIIVWGLELDTYLSNILINLYSRAGGMVYARKVFEKMPERNLVSWSTMVSACNHHGI 125

Query: 206 SREAIEVF-RLMQLEGFKGDDFTFSSLLSSCK-YKGSGE-LGKQLHCLLIKQSFDLDILV 265
             E++ VF    +      +++  SS + +C    G G  +  QL   L+K  FD D+ V
Sbjct: 126 YEESLVVFLEFWRTRKDSPNEYILSSFIQACSGLDGRGRWMVFQLQSFLVKSGFDRDVYV 185

Query: 266 ASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDY 325
            + L++ Y K+ N+  AR VFD +P +++V+WTTMI G  +      +++LF ++   + 
Sbjct: 186 GTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMGRSYVSLQLFYQLMEDNV 245

Query: 326 CPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQ 385
            PD    ++VLS+C          Q+H+ +++ G E   S+ N LI +Y KCG + AA +
Sbjct: 246 VPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLEMDASLMNVLIDSYVKCGRVIAAHK 305

Query: 386 CFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGF 445
            F  +   ++++WT+++ G     L K+A++LF  M  +G++PD  A   +L++C+    
Sbjct: 306 LFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSMSKFGLKPDMYACSSILTSCASLHA 365

Query: 446 VSMG--LHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQA 483
           +  G  +H + +  N   L  DS     LID+  +   L  A
Sbjct: 366 LGFGTQVHAYTIKAN---LGNDSYVTNSLIDMYAKCDCLTDA 404


HSP 5 Score: 112.8 bits (281), Expect = 7.3e-25
Identity = 89/311 (28.62%), Postives = 145/311 (46.62%), Query Frame = 1

Query: 247 LHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEY 306
           +H  +I    +LD  +++ L+N+Y++   +  ARKVF++MP RN VSW+TM+     H  
Sbjct: 66  VHGQIIVWGLELDTYLSNILINLYSRAGGMVYARKVFEKMPERNLVSWSTMVSACNHHGI 125

Query: 307 GKEAVKLFRRMFR-KDYCPDELTFASVLSSCGFTSGASELM--QVHSCLIKLGFEAFLSI 366
            +E++ +F   +R +   P+E   +S + +C    G    M  Q+ S L+K GF+  + +
Sbjct: 126 YEESLVVFLEFWRTRKDSPNEYILSSFIQACSGLDGRGRWMVFQLQSFLVKSGFDRDVYV 185

Query: 367 NNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGI 426
              LI  Y K G I  A   F  + E   VTWT++I G    G    +++LF +++   +
Sbjct: 186 GTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMGRSYVSLQLFYQLMEDNV 245

Query: 427 RPDKIAFLGVLSACSHGGFVSMG--LHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQ 486
            PD      VLSACS   F+  G  +H   L   +Y L  D+  +  LID   + G +  
Sbjct: 246 VPDGYILSTVLSACSILPFLEGGKQIHAHIL---RYGLEMDASLMNVLIDSYVKCGRVIA 305

Query: 487 AFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASEG 546
           A  L   MP +     + ++      +    L K AME  +   +   + L  +MYA   
Sbjct: 306 AHKLFNGMPNK----NIISWTTLLSGYKQNALHKEAMELFTSMSK---FGLKPDMYACSS 365

Query: 547 RWSDVARMRKL 553
             +  A +  L
Sbjct: 366 ILTSCASLHAL 366

BLAST of Csa6G005130 vs. TAIR10
Match: AT4G21300.1 (AT4G21300.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 334.0 bits (855), Expect = 2.0e-91
Identity = 196/598 (32.78%), Postives = 317/598 (53.01%), Query Frame = 1

Query: 22  VLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRN 81
           VL   A    I     LH LVV  G+  E S++N LL +Y KC   D A  LF  M+R +
Sbjct: 245 VLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIKNSLLSMYSKCGRFDDASKLFRMMSRAD 304

Query: 82  VVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVE 141
            V+WN +I G V  G      + + S+  +++ +  G++ PD ITF+ L  S     ++E
Sbjct: 305 TVTWNCMISGYVQSG------LMEESLTFFYEMISSGVL-PDAITFSSLLPSVSKFENLE 364

Query: 142 SGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCCV 201
             +Q+H ++M+    LD F+ SA++D Y KC     A+  FS     D+V++  M+   +
Sbjct: 365 YCKQIHCYIMRHSISLDIFLTSALIDAYFKCRGVSMAQNIFSQCNSVDVVVFTAMISGYL 424

Query: 202 FNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDIL 261
            N L  +++E+FR +       ++ T  S+L       + +LG++LH  +IK+ FD    
Sbjct: 425 HNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILLALKLGRELHGFIIKKGFDNRCN 484

Query: 262 VASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKD 321
           +  +++++Y K   +  A ++F+ +  R+ VSW +MI    Q +    A+ +FR+M    
Sbjct: 485 IGCAVIDMYAKCGRMNLAYEIFERLSKRDIVSWNSMITRCAQSDNPSAAIDIFRQMGVSG 544

Query: 322 YCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAAL 381
            C D ++ ++ LS+C      S    +H  +IK    + +   + LI  Y+KCG + AA+
Sbjct: 545 ICYDCVSISAALSACANLPSESFGKAIHGFMIKHSLASDVYSESTLIDMYAKCGNLKAAM 604

Query: 382 QCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKML-SYGIRPDKIAFLGVLSACSHG 441
             F+ + E ++V+W SII      G  KD++ LF +M+   GIRPD+I FL ++S+C H 
Sbjct: 605 NVFKTMKEKNIVSWNSIIAACGNHGKLKDSLCLFHEMVEKSGIRPDQITFLEIISSCCHV 664

Query: 442 GFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRA 501
           G V  G+ +F  MT  Y + P  EH  C++DL GRAG L +A++ +KSMP          
Sbjct: 665 GDVDEGVRFFRSMTEDYGIQPQQEHYACVVDLFGRAGRLTEAYETVKSMPFPPDAGVWGT 724

Query: 502 FIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDRCE 561
            + ACR H N+ LA+ A       +P     Y L+SN +A+   W  V ++R L+ +R  
Sbjct: 725 LLGACRLHKNVELAEVASSKLMDLDPSNSGYYVLISNAHANAREWESVTKVRSLMKEREV 784

Query: 562 QKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTMKKDYKFTASQVDIVPE 617
           QK PG SW+EI    HLF+SGD +HP+S  +Y++L  LL  ++ +       + + PE
Sbjct: 785 QKIPGYSWIEINKRTHLFVSGDVNHPESSHIYSLLNSLLGELRLEGYIPQPYLPLHPE 835


HSP 2 Score: 209.1 bits (531), Expect = 7.5e-54
Identity = 143/504 (28.37%), Postives = 243/504 (48.21%), Query Frame = 1

Query: 9   FGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCRDLD 68
           FG S  V +F   ++KA   + +      L   V  LG+     V + L++ Y++   +D
Sbjct: 132 FGVSPDVSTFPC-LVKACVALKNFKGIDFLSDTVSSLGMDCNEFVASSLIKAYLEYGKID 191

Query: 69  SARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGITFN 128
               LFD + +++ V WN ++ G    G          S+   F  M M  + P+ +TF+
Sbjct: 192 VPSKLFDRVLQKDCVIWNVMLNGYAKCG-------ALDSVIKGFSVMRMDQISPNAVTFD 251

Query: 129 GLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCILYR 188
            +   C     ++ G QLH  V+  G D +  + ++++  Y+KCG ++DA   F  +   
Sbjct: 252 CVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIKNSLLSMYSKCGRFDDASKLFRMMSRA 311

Query: 189 DLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLH 248
           D V WN M+   V + L  E++  F  M   G   D  TFSSLL S     + E  KQ+H
Sbjct: 312 DTVTWNCMISGYVQSGLMEESLTFFYEMISSGVLPDAITFSSLLPSVSKFENLEYCKQIH 371

Query: 249 CLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGK 308
           C +++ S  LDI + S+L++ Y K   +  A+ +F +  + + V +T MI GY  +    
Sbjct: 372 CYIMRHSISLDIFLTSALIDAYFKCRGVSMAQNIFSQCNSVDVVVFTAMISGYLHNGLYI 431

Query: 309 EAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINNGLI 368
           +++++FR + +    P+E+T  S+L   G         ++H  +IK GF+   +I   +I
Sbjct: 432 DSLEMFRWLVKVKISPNEITLVSILPVIGILLALKLGRELHGFIIKKGFDNRCNIGCAVI 491

Query: 369 YAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRPDKI 428
             Y+KCG +  A + F  +++ D+V+W S+I   A       A+ +F +M   GI  D +
Sbjct: 492 DMYAKCGRMNLAYEIFERLSKRDIVSWNSMITRCAQSDNPSAAIDIFRQMGVSGICYDCV 551

Query: 429 AFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKS 488
           +    LSAC++    S G      M  ++ L  D    + LID+  + G+L  A ++ K+
Sbjct: 552 SISAALSACANLPSESFGKAIHGFMI-KHSLASDVYSESTLIDMYAKCGNLKAAMNVFKT 611

Query: 489 MPKEAGPDALRAFIRACRTHGNLR 513
           M KE    +  + I AC  HG L+
Sbjct: 612 M-KEKNIVSWNSIIAACGNHGKLK 625


HSP 3 Score: 132.5 bits (332), Expect = 8.9e-31
Identity = 90/315 (28.57%), Postives = 146/315 (46.35%), Query Frame = 1

Query: 130 LFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDA-RLAFSCILYR 189
           L ++C   N +  G+Q+H+F++      D +    ++  YA CG + D  ++ +   L R
Sbjct: 41  LLQACSNPNLLRQGKQVHAFLIVNSISGDSYTDERILGMYAMCGSFSDCGKMFYRLDLRR 100

Query: 190 DLVL-WNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSC----KYKGSGEL 249
             +  WN ++   V N L  +A+  +  M   G   D  TF  L+ +C     +KG   L
Sbjct: 101 SSIRPWNSIISSFVRNGLLNQALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKGIDFL 160

Query: 250 GKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQ 309
              +  L      D +  VASSL+  Y +   +    K+FD +  ++ V W  M+ GY +
Sbjct: 161 SDTVSSL----GMDCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQKDCVIWNVMLNGYAK 220

Query: 310 HEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSI 369
                  +K F  M      P+ +TF  VLS C         +Q+H  ++  G +   SI
Sbjct: 221 CGALDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSI 280

Query: 370 NNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGI 429
            N L+  YSKCG    A + FR+++  D VTW  +I G    GL ++++  F +M+S G+
Sbjct: 281 KNSLLSMYSKCGRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFYEMISSGV 340

Query: 430 RPDKIAFLGVLSACS 439
            PD I F  +L + S
Sbjct: 341 LPDAITFSSLLPSVS 351

BLAST of Csa6G005130 vs. NCBI nr
Match: gi|778709135|ref|XP_011656346.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 616/616 (100.00%), Postives = 616/616 (100.00%), Query Frame = 1

Query: 1   MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRV 60
           MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRV
Sbjct: 1   MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRV 60

Query: 61  YVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLV 120
           YVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLV
Sbjct: 61  YVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLV 120

Query: 121 DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARL 180
           DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARL
Sbjct: 121 DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARL 180

Query: 181 AFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGS 240
           AFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGS
Sbjct: 181 AFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGS 240

Query: 241 GELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVG 300
           GELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVG
Sbjct: 241 GELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVG 300

Query: 301 YGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF 360
           YGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF
Sbjct: 301 YGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF 360

Query: 361 LSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLS 420
           LSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLS
Sbjct: 361 LSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLS 420

Query: 421 YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLD 480
           YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLD
Sbjct: 421 YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLD 480

Query: 481 QAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASE 540
           QAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASE
Sbjct: 481 QAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASE 540

Query: 541 GRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTM 600
           GRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTM
Sbjct: 541 GRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTM 600

Query: 601 KKDYKFTASQVDIVPE 617
           KKDYKFTASQVDIVPE
Sbjct: 601 KKDYKFTASQVDIVPE 616

BLAST of Csa6G005130 vs. NCBI nr
Match: gi|659116662|ref|XP_008458191.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 572/608 (94.08%), Postives = 583/608 (95.89%), Query Frame = 1

Query: 1   MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRV 60
           MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIP DTLLHS+VVKLGL NELSVQNKLL+V
Sbjct: 11  MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPRDTLLHSVVVKLGLANELSVQNKLLKV 70

Query: 61  YVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLV 120
           YVKCRDLDSAR+LFDEM RRN VSWNTVICGLVDGGYGGEFK RQ  IFLYFKKMLMGLV
Sbjct: 71  YVKCRDLDSARSLFDEMPRRNAVSWNTVICGLVDGGYGGEFKTRQRLIFLYFKKMLMGLV 130

Query: 121 DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARL 180
           DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSA+VDFYAKCGLYEDARL
Sbjct: 131 DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSALVDFYAKCGLYEDARL 190

Query: 181 AFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGS 240
           AFSC LY+DLVLWNVMLYC VFNSLSREAIE FRLMQLEGFKGD+FTFSSLLSSCKYKGS
Sbjct: 191 AFSCTLYKDLVLWNVMLYCYVFNSLSREAIEGFRLMQLEGFKGDEFTFSSLLSSCKYKGS 250

Query: 241 GELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVG 300
           GELGKQLH LLIKQSFDLDILVASSL++VY KNDNLYDARKVFDEMPTRNSVSWTTMIVG
Sbjct: 251 GELGKQLHGLLIKQSFDLDILVASSLIDVYAKNDNLYDARKVFDEMPTRNSVSWTTMIVG 310

Query: 301 YGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF 360
           YGQ EYGKEAVKLFRRMF KDYC DELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF
Sbjct: 311 YGQQEYGKEAVKLFRRMFGKDYCLDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF 370

Query: 361 LSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLS 420
           LSINNGLIYAYSKCGI+AAALQCFRLIAEPDLVTWTSIICGLA CGLEKDAVKLFDKMLS
Sbjct: 371 LSINNGLIYAYSKCGIVAAALQCFRLIAEPDLVTWTSIICGLAFCGLEKDAVKLFDKMLS 430

Query: 421 YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLD 480
           YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPD EHLTCLIDLLGRAGSLD
Sbjct: 431 YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDPEHLTCLIDLLGRAGSLD 490

Query: 481 QAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASE 540
           QAFDLLKSM KEAGPDAL AFIRACRTHGNL+LAK AMEF SEPDEPVNYSLVSNMYASE
Sbjct: 491 QAFDLLKSMRKEAGPDALTAFIRACRTHGNLKLAKWAMEFISEPDEPVNYSLVSNMYASE 550

Query: 541 GRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTM 600
           GRWSDVARM KLINDRCEQKTPGLSWVEIAGYNHLF SGDRSHPQS DLYAMLGLLLNTM
Sbjct: 551 GRWSDVARMHKLINDRCEQKTPGLSWVEIAGYNHLFKSGDRSHPQSSDLYAMLGLLLNTM 610

Query: 601 KKDYKFTA 609
           K+DYK TA
Sbjct: 611 KEDYKSTA 618

BLAST of Csa6G005130 vs. NCBI nr
Match: gi|659116664|ref|XP_008458193.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 546/579 (94.30%), Postives = 556/579 (96.03%), Query Frame = 1

Query: 1   MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRV 60
           MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIP DTLLHS+VVKLGL NELSVQNKLL+V
Sbjct: 11  MLIWTSTHFGRSRLVHSFSFNVLKAAAPVNSIPRDTLLHSVVVKLGLANELSVQNKLLKV 70

Query: 61  YVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLV 120
           YVKCRDLDSAR+LFDEM RRN VSWNTVICGLVDGGYGGEFK RQ  IFLYFKKMLMGLV
Sbjct: 71  YVKCRDLDSARSLFDEMPRRNAVSWNTVICGLVDGGYGGEFKTRQRLIFLYFKKMLMGLV 130

Query: 121 DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARL 180
           DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSA+VDFYAKCGLYEDARL
Sbjct: 131 DPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSALVDFYAKCGLYEDARL 190

Query: 181 AFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGS 240
           AFSC LY+DLVLWNVMLYC VFNSLSREAIE FRLMQLEGFKGD+FTFSSLLSSCKYKGS
Sbjct: 191 AFSCTLYKDLVLWNVMLYCYVFNSLSREAIEGFRLMQLEGFKGDEFTFSSLLSSCKYKGS 250

Query: 241 GELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVG 300
           GELGKQLH LLIKQSFDLDILVASSL++VY KNDNLYDARKVFDEMPTRNSVSWTTMIVG
Sbjct: 251 GELGKQLHGLLIKQSFDLDILVASSLIDVYAKNDNLYDARKVFDEMPTRNSVSWTTMIVG 310

Query: 301 YGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF 360
           YGQ EYGKEAVKLFRRMF KDYC DELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF
Sbjct: 311 YGQQEYGKEAVKLFRRMFGKDYCLDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAF 370

Query: 361 LSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLS 420
           LSINNGLIYAYSKCGI+AAALQCFRLIAEPDLVTWTSIICGLA CGLEKDAVKLFDKMLS
Sbjct: 371 LSINNGLIYAYSKCGIVAAALQCFRLIAEPDLVTWTSIICGLAFCGLEKDAVKLFDKMLS 430

Query: 421 YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLD 480
           YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPD EHLTCLIDLLGRAGSLD
Sbjct: 431 YGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDPEHLTCLIDLLGRAGSLD 490

Query: 481 QAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAKRAMEFASEPDEPVNYSLVSNMYASE 540
           QAFDLLKSM KEAGPDAL AFIRACRTHGNL+LAK AMEF SEPDEPVNYSLVSNMYASE
Sbjct: 491 QAFDLLKSMRKEAGPDALTAFIRACRTHGNLKLAKWAMEFISEPDEPVNYSLVSNMYASE 550

Query: 541 GRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISG 580
           GRWSDVARM KLINDRCEQKTPGLSWVEIAGYNHLF SG
Sbjct: 551 GRWSDVARMHKLINDRCEQKTPGLSWVEIAGYNHLFKSG 589

BLAST of Csa6G005130 vs. NCBI nr
Match: gi|778709139|ref|XP_011656347.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 811.2 bits (2094), Expect = 1.2e-231
Identity = 400/401 (99.75%), Postives = 401/401 (100.00%), Query Frame = 1

Query: 216 MQLEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDN 275
           M+LEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDN
Sbjct: 4   MELEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDN 63

Query: 276 LYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSS 335
           LYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSS
Sbjct: 64  LYDARKVFDEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSS 123

Query: 336 CGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTW 395
           CGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTW
Sbjct: 124 CGFTSGASELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTW 183

Query: 396 TSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTN 455
           TSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTN
Sbjct: 184 TSIICGLALCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTN 243

Query: 456 QYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAK 515
           QYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAK
Sbjct: 244 QYQLVPDSEHLTCLIDLLGRAGSLDQAFDLLKSMPKEAGPDALRAFIRACRTHGNLRLAK 303

Query: 516 RAMEFASEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHL 575
           RAMEFASEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHL
Sbjct: 304 RAMEFASEPDEPVNYSLVSNMYASEGRWSDVARMRKLINDRCEQKTPGLSWVEIAGYNHL 363

Query: 576 FISGDRSHPQSLDLYAMLGLLLNTMKKDYKFTASQVDIVPE 617
           FISGDRSHPQSLDLYAMLGLLLNTMKKDYKFTASQVDIVPE
Sbjct: 364 FISGDRSHPQSLDLYAMLGLLLNTMKKDYKFTASQVDIVPE 404

BLAST of Csa6G005130 vs. NCBI nr
Match: gi|778709139|ref|XP_011656347.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 122.9 bits (307), Expect = 2.0e-24
Identity = 75/221 (33.94%), Postives = 115/221 (52.04%), Query Frame = 1

Query: 123 DGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAF 182
           D  TF+ L  SC      E G+QLH  ++K  FDLD  V S++V+ Y K     DAR  F
Sbjct: 12  DDFTFSSLLSSCKYKGSGELGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVF 71

Query: 183 SCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGE 242
             +  R+ V W  M+     +   +EA+++FR M  + +  D+ TF+S+LSSC +     
Sbjct: 72  DEMPTRNSVSWTTMIVGYGQHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGAS 131

Query: 243 LGKQLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYG 302
              Q+H  LIK  F+  + + + L+  Y+K   +  A + F  +   + V+WT++I G  
Sbjct: 132 ELMQVHSCLIKLGFEAFLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLA 191

Query: 303 QHEYGKEAVKLFRRMFRKDYCPDELTFASVLSSC---GFTS 341
                K+AVKLF +M      PD++ F  VLS+C   GF S
Sbjct: 192 LCGLEKDAVKLFDKMLSYGIRPDKIAFLGVLSACSHGGFVS 232


HSP 2 Score: 109.0 bits (271), Expect = 3.0e-20
Identity = 66/217 (30.41%), Postives = 104/217 (47.93%), Query Frame = 1

Query: 38  LHSLVVKLGLVNELSVQNKLLRVYVKCRDLDSARNLFDEMARRNVVSWNTVICGLVDGGY 97
           LH L++K     ++ V + L+ VY K  +L  AR +FDEM  RN VSW T+I G     Y
Sbjct: 35  LHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHEY 94

Query: 98  GGEFKMRQHSIFLYFKKMLMGLVDPDGITFNGLFRSCVVLNDVESGRQLHSFVMKIGFDL 157
           G E           F++M      PD +TF  +  SC   +      Q+HS ++K+GF+ 
Sbjct: 95  GKE-------AVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEA 154

Query: 158 DCFVGSAVVDFYAKCGLYEDARLAFSCILYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQ 217
              + + ++  Y+KCG+   A   F  I   DLV W  ++       L ++A+++F  M 
Sbjct: 155 FLSINNGLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKML 214

Query: 218 LEGFKGDDFTFSSLLSSCKYKGSGELGKQLHCLLIKQ 255
             G + D   F  +LS+C + G   +G     L+  Q
Sbjct: 215 SYGIRPDKIAFLGVLSACSHGGFVSMGLHYFNLMTNQ 244


HSP 3 Score: 698.4 bits (1801), Expect = 1.2e-197
Identity = 348/599 (58.10%), Postives = 442/599 (73.79%), Query Frame = 1

Query: 6   STHFGRSRLVHSFSFNVLKAAAPVNSIPHDTLLHSLVVKLGLVNELSVQNKLLRVYVKCR 65
           STHF      HSF  N LK +A +  +     LH  VVKLGL N  S+Q ++L VY+KC+
Sbjct: 64  STHFNDPHSAHSFCSNALKVSAKMGFLREGKQLHGHVVKLGLYNVQSLQIQILNVYLKCK 123

Query: 66  DLDSARNLFDEMARRNVVSWNTVICGLVDGGYGGEFKMRQHSIFLYFKKMLMGLVDPDGI 125
           D ++A+ LF EM +RNVV+WNT+I GLV+    G ++ + +  F YF++ML+  V PD I
Sbjct: 124 DFNNAQRLFGEMRKRNVVAWNTLISGLVN--CWGNYESKLYLGFSYFRRMLLEAVGPDDI 183

Query: 126 TFNGLFRSCVVLNDVESGRQLHSFVMKIGFDLDCFVGSAVVDFYAKCGLYEDARLAFSCI 185
           TFNGLFR CV LNDVE GRQLH FV+K+GF  +CFVGSA+VD YAK GL  +AR AF  +
Sbjct: 184 TFNGLFRVCVDLNDVEIGRQLHCFVVKLGFGSNCFVGSALVDLYAKHGLIXNARCAFDFV 243

Query: 186 LYRDLVLWNVMLYCCVFNSLSREAIEVFRLMQLEGFKGDDFTFSSLLSSCKYKGSGELGK 245
           LYRDLVLWNVM+YC   NSL++EA  VF LM+LEG KGD+FTFSSLLSSC+  GS + GK
Sbjct: 244 LYRDLVLWNVMVYCYASNSLAKEAFGVFNLMRLEGVKGDEFTFSSLLSSCRTLGSCKPGK 303

Query: 246 QLHCLLIKQSFDLDILVASSLVNVYTKNDNLYDARKVFDEMPTRNSVSWTTMIVGYGQHE 305
           Q+H ++I+++FD D+LV+S+LV++Y KND++ DA K FD M  RN VSWTT+IVGYG H 
Sbjct: 304 QIHGIIIREAFDSDVLVSSALVDMYAKNDDIGDAWKAFDAMSIRNVVSWTTVIVGYGLHG 363

Query: 306 YGKEAVKLFRRMFRKDYCPDELTFASVLSSCGFTSGASELMQVHSCLIKLGFEAFLSINN 365
             KEA+ L R MFR+   PDELT AS++SSCG  S ASELMQVH+ ++K GF  F SI N
Sbjct: 364 KEKEAIGLLREMFREHLYPDELTLASIVSSCGNVSSASELMQVHAYMVKFGFHFFSSIAN 423

Query: 366 GLIYAYSKCGIIAAALQCFRLIAEPDLVTWTSIICGLALCGLEKDAVKLFDKMLSYGIRP 425
            LI AYSKCG I++A +CF L+ EPDLVTWTS+IC  A   L ++A ++F+KML+Y I P
Sbjct: 424 SLITAYSKCGSISSASKCFNLVVEPDLVTWTSLICAYAFHSLAEEATEVFEKMLAYDIMP 483

Query: 426 DKIAFLGVLSACSHGGFVSMGLHYFNLMTNQYQLVPDSEHLTCLIDLLGRAGSLDQAFDL 485
           D+IAFL VLSACSHGG +  GLHYF LM+N YQ+ PDSEH TCLIDLLGRAG LD+AF  
Sbjct: 484 DQIAFLAVLSACSHGGLIQKGLHYFKLMSNDYQIFPDSEHYTCLIDLLGRAGLLDEAFMA 543

Query: 486 LKSMPKEAGPDALRAFIRACRTHGNLRLAKRAME--FASEPDEPVNYSLVSNMYASEGRW 545
           L SMP E  P  L AF+ AC+ HGN+ LAK A +  FA EP++PVNY+L+SN+Y+S+G W
Sbjct: 544 LTSMPIEPDPSTLGAFMGACKVHGNIELAKWAAQKLFALEPNKPVNYTLMSNIYSSQGHW 603

Query: 546 SDVARMRKLINDRCEQKTPGLSWVEIAGYNHLFISGDRSHPQSLDLYAMLGLLLNTMKK 603
            DV+R+RK++   C+ K PG +WVEI G    F+ GD SHPQ+ ++YAMLGLLL  MK+
Sbjct: 604 GDVSRVRKMMRHSCDYKAPGCNWVEIGGGICTFVPGDESHPQAPEVYAMLGLLLRLMKE 660

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP203_ARATH1.4e-12645.13Pentatricopeptide repeat-containing protein At2g46050, mitochondrial OS=Arabidop... [more]
PP151_ARATH1.0e-10032.65Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana GN... [more]
PP320_ARATH2.7e-9333.05Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... [more]
PP357_ARATH1.6e-9033.91Pentatricopeptide repeat-containing protein At4g39530 OS=Arabidopsis thaliana GN... [more]
PP333_ARATH3.6e-9032.78Pentatricopeptide repeat-containing protein At4g21300 OS=Arabidopsis thaliana GN... [more]
Match NameE-valueIdentityDescription
A0A0A0K863_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G005130 PE=4 SV=1[more]
A0A067HEI7_CITSI4.2e-18656.44Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g044628mg PE=4 SV=1[more]
F6I669_VITVI1.7e-18455.42Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0046g01830 PE=4 SV=... [more]
W9T0A0_9ROSA3.0e-18454.47Uncharacterized protein OS=Morus notabilis GN=L484_021461 PE=4 SV=1[more]
A5BLE5_VITVI1.1e-17854.24Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010801 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G46050.17.9e-12845.13 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT2G13600.15.7e-10232.65 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT4G18750.11.5e-9433.05 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT4G39530.19.1e-9233.91 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT4G21300.12.0e-9132.78 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778709135|ref|XP_011656346.1|0.0e+00100.00PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial ... [more]
gi|659116662|ref|XP_008458191.1|0.0e+0094.08PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial ... [more]
gi|659116664|ref|XP_008458193.1|0.0e+0094.30PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial ... [more]
gi|778709139|ref|XP_011656347.1|1.2e-23199.75PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial ... [more]
gi|778709139|ref|XP_011656347.1|2.0e-2433.94PREDICTED: pentatricopeptide repeat-containing protein At2g46050, mitochondrial ... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G005130.1Csa6G005130.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 467..491
score: 0.01coord: 264..289
score: 0.004coord: 55..82
score: 0.
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 290..336
score: 2.1E-9coord: 189..235
score: 8.9E-8coord: 390..437
score: 8.
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 467..491
score: 0.002coord: 393..426
score: 3.8E-7coord: 191..224
score: 6.6E-4coord: 54..83
score: 3.7E-4coord: 292..326
score: 5.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 123..157
score: 7.026coord: 290..324
score: 10.764coord: 224..258
score: 6.478coord: 259..289
score: 7.662coord: 189..223
score: 8.923coord: 391..425
score: 11.707coord: 325..359
score: 6.16coord: 462..492
score: 8.1coord: 426..456
score: 5.437coord: 50..84
score: 9.208coord: 158..188
score: 5.075coord: 526..556
score: 5
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 55..567
score: 1.0E
NoneNo IPR availablePANTHERPTHR24015:SF834SUBFAMILY NOT NAMEDcoord: 55..567
score: 1.0E

The following gene(s) are paralogous to this gene:

None