Csa5G648640 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa5G648640
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Tir-nbs-lrr resistance protein; contains IPR000157 (Toll/interleukin-1 receptor homology (TIR) domain)
Location	Chr5 : 27489586 .. 27490327 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCAATGGTCCCTTCTTCTCCTTCTTCTTCTGCTGCTTCTTCTTCTTCTCCTTCTTCTTCTCCTTCAATTGGTAAATGGAAATTTGATGTGTTCTTGAGCTTTCGAGGCGAAGATACACGTGGTGGCTTCACGGATCATCTCTACAAAGCCTTAACACGAAAGGGAATTTCAACATTTAGAGATGAAAATGAGATCGAAGAAGGTGAACACATTCCTTCAAATCTGCTGGCTTCCATTGATGCCTCGAGATTTGCCATTGTTGTGGTTTCGGAAGATTATGCATCTTCAAGATGGTGCCTCGAGGAATTGGCTAGGATGTTTGAATGTAAAAAAGAAGTTTTACCAATTTTTTATAAAGTGGATCCCTCTCATGTGAAAAACCAAAGTGGAACGTTTGAAGAAGCTTTTGTTAAACATGAAAAGAGATTTGGAAGAGGTGATGGGAAGGTTCAAAGTTGGAGGACGTTTCTCACCGAGCTTGCTAACACCAAAGCTTGGCTTTCTCAATCTTGGTAATTAACTTAACTATTTATCATTTAAATTCTTAGTATATTGTAAATATAGATGGTGATATCCTAATATATTTTATGTAGCCACTTGGATTGAAATTTGGAAGAGAAATTTAGGCAAAAAATTTGCTCCATTTTTTTTTTCTTTTTCTTCTAATTTTCTTTTGTCTTCTAGATCTCTTTCACAATTAATATATGCATTTTAGTTTTAATCAAATATATAAAATTTTCC

mRNA sequence

ATGGTCCCTTCTTCTCCTTCTTCTTCTGCTGCTTCTTCTTCTTCTCCTTCTTCTTCTCCTTCAATTGGTAAATGGAAATTTGATGTGTTCTTGAGCTTTCGAGGCGAAGATACACGTGGTGGCTTCACGGATCATCTCTACAAAGCCTTAACACGAAAGGGAATTTCAACATTTAGAGATGAAAATGAGATCGAAGAAGGTGAACACATTCCTTCAAATCTGCTGGCTTCCATTGATGCCTCGAGATTTGCCATTGTTGTGGTTTCGGAAGATTATGCATCTTCAAGATGGTGCCTCGAGGAATTGGCTAGGATGTTTGAATGTAAAAAAGAAGTTTTACCAATTTTTTATAAAGTGGATCCCTCTCATGTGAAAAACCAAAGTGGAACGTTTGAAGAAGCTTTTGTTAAACATGAAAAGAGATTTGGAAGAGGTGATGGGAAGGTTCAAAGTTGGAGGACGTTTCTCACCGAGCTTGCTAACACCAAAGCTTGGCTTTCTCAATCTTGGTAA

Coding sequence (CDS)

ATGGTCCCTTCTTCTCCTTCTTCTTCTGCTGCTTCTTCTTCTTCTCCTTCTTCTTCTCCTTCAATTGGTAAATGGAAATTTGATGTGTTCTTGAGCTTTCGAGGCGAAGATACACGTGGTGGCTTCACGGATCATCTCTACAAAGCCTTAACACGAAAGGGAATTTCAACATTTAGAGATGAAAATGAGATCGAAGAAGGTGAACACATTCCTTCAAATCTGCTGGCTTCCATTGATGCCTCGAGATTTGCCATTGTTGTGGTTTCGGAAGATTATGCATCTTCAAGATGGTGCCTCGAGGAATTGGCTAGGATGTTTGAATGTAAAAAAGAAGTTTTACCAATTTTTTATAAAGTGGATCCCTCTCATGTGAAAAACCAAAGTGGAACGTTTGAAGAAGCTTTTGTTAAACATGAAAAGAGATTTGGAAGAGGTGATGGGAAGGTTCAAAGTTGGAGGACGTTTCTCACCGAGCTTGCTAACACCAAAGCTTGGCTTTCTCAATCTTGGTAA

Protein sequence

MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVDPSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW*

Homology

[Close]

BLAST of Csa5G648640 vs. Swiss-Prot
Match: TMVRN_NICGU (TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.3e-39
Identity = 80/151 (52.98%), Postives = 101/151 (66.89%), Query Frame = 1


Query: 17  SSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPSNLLA 76
           +SS S  +W +DVFLSFRGEDTR  FT HLY+ L  KGI TF+D+  +E G  IP  L  
Sbjct: 2   ASSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCK 61

Query: 77  SIDASRFAIVVVSEDYASSRWCLEELARMFEC----KKEVLPIFYKVDPSHVKNQSGTFE 136
           +I+ S+FAIVV SE+YA+SRWCL EL ++ EC    K+ V+PIFY VDPSHV+NQ  +F 
Sbjct: 62  AIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFA 121

Query: 137 EAFVKHEKRFGRGDGKVQSWRTFLTELANTK 164
           +AF +HE ++      +Q WR  L E AN K
Sbjct: 122 KAFEEHETKYKDDVEGIQRWRIALNEAANLK 152

[Close]

BLAST of Csa5G648640 vs. Swiss-Prot
Match: TIR_ARATH (Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana GN=TIR PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.4e-30
Identity = 68/139 (48.92%), Postives = 87/139 (62.59%), Query Frame = 1


Query: 26  KFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPSNLLASIDASRFAI 85
           K+DVFLSFRG DTR  F   LYK L R+ I TF+D+ E+E G+     L + I+ SRFA+
Sbjct: 8   KYDVFLSFRGHDTRHNFISFLYKELVRRSIRTFKDDKELENGQRFSPELKSPIEVSRFAV 67

Query: 86  VVVSEDYASSRWCLEELARMFECKKE----VLPIFYKVDPSHVKNQSGTFEEAFVKHEKR 145
           VVVSE+YA+S WCL+EL  + + +K+    V+PIFY V+P+HV+ Q+G   E F KH  R
Sbjct: 68  VVVSENYAASSWCLDELVTIMDFEKKGSITVMPIFYGVEPNHVRWQTGVLAEQFKKHASR 127

Query: 146 FGRGDGKVQSWRTFLTELA 161
                 KV  WR  LT  A
Sbjct: 128 --EDPEKVLKWRQALTNFA 144

[Close]

BLAST of Csa5G648640 vs. Swiss-Prot
Match: Y4117_ARATH (Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g11170 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.9e-29
Identity = 68/157 (43.31%), Postives = 95/157 (60.51%), Query Frame = 1


Query: 18  SSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPSNLLAS 77
           +S S   W++DVF SFRGED R  F  HL K    KGI TFRD++ I+    I   L A+
Sbjct: 2   ASSSSNSWRYDVFPSFRGEDVRNNFLSHLLKEFESKGIVTFRDDH-IKRSHTIGHELRAA 61

Query: 78  IDASRFAIVVVSEDYASSRWCLEELARMFECKKE----VLPIFYKVDPSHVKNQSGTFEE 137
           I  S+ ++V+ SE+YASS WCL+EL  + +CK+E    V+P+FYKVDPS ++ Q+G F  
Sbjct: 62  IRESKISVVLFSENYASSSWCLDELIEIMKCKEEQGLKVMPVFYKVDPSDIRKQTGKFGM 121

Query: 138 AFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           +F+  E   G+ + +  +WR  LT+ AN      Q+W
Sbjct: 122 SFL--ETCCGKTEERQHNWRRALTDAANILGDHPQNW 155

[Close]

BLAST of Csa5G648640 vs. Swiss-Prot
Match: TAO1_ARATH (Disease resistance protein TAO1 OS=Arabidopsis thaliana GN=TAO1 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-28
Identity = 74/167 (44.31%), Postives = 98/167 (58.68%), Query Frame = 1


Query: 8   SSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEG 67
           SS+ SSSSP SS S   W   VFLSFRGED R G   H+ K   R GI+ F D NE++ G
Sbjct: 22  SSSLSSSSPPSSLS-QNWLHPVFLSFRGEDVRKGLLSHIQKEFQRNGITPFID-NEMKRG 81

Query: 68  EHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKE----VLPIFYKVDPSH 127
             I   LL +I  S+ AI+++S +Y SS+WCL+EL  + +C++E    V+ +FY VDPS 
Sbjct: 82  GSIGPELLQAIRGSKIAIILLSRNYGSSKWCLDELVEIMKCREELGQTVMTVFYDVDPSD 141

Query: 128 VKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           V+ Q G F + F K     GR +  VQ W+  LT  AN     S++W
Sbjct: 142 VRKQKGDFGKVFKK--TCVGRPEEMVQRWKQALTSAANILGEDSRNW 184

[Close]

BLAST of Csa5G648640 vs. Swiss-Prot
Match: RPP1_ARATH (Probable disease resistance protein RPP1 OS=Arabidopsis thaliana GN=RPP1 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 7.0e-28
Identity = 70/168 (41.67%), Postives = 100/168 (59.52%), Query Frame = 1


Query: 8   SSAASSSSPSSSPSIGK-WKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEE 67
           S+++S S PS + S+ + WK DVF SF G D R  F  H+ ++  RKGI TF D N IE 
Sbjct: 78  SNSSSLSLPSPATSVSRNWKHDVFPSFHGADVRRTFLSHIMESFRRKGIDTFIDNN-IER 137

Query: 68  GEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKE----VLPIFYKVDPS 127
            + I   L  +I  S+ AIV++S  YASS WCL+ELA + +C++     V+ IFY+VDP+
Sbjct: 138 SKSIGPELKEAIKGSKIAIVLLSRKYASSSWCLDELAEIMKCRQMVGQIVMTIFYEVDPT 197

Query: 128 HVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
            +K Q+G F +AF K  +  G+   +V+ WR  L ++A    + S SW
Sbjct: 198 DIKKQTGEFGKAFTKTCR--GKPKEQVERWRKALEDVATIAGYHSHSW 242

[Close]

BLAST of Csa5G648640 vs. TrEMBL
Match: A0A0A0KSU0_CUCSA (MRGH14 OS=Cucumis sativus GN=Csa_5G648640 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 1.3e-92
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 1


Query: 1   MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD 60
           MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD
Sbjct: 1   MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD 60

Query: 61  ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD 120
           ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD
Sbjct: 61  ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD 120

Query: 121 PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW
Sbjct: 121 PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 170

[Close]

BLAST of Csa5G648640 vs. TrEMBL
Match: M4R4K0_CUCME (RGH14 OS=Cucumis melo GN=RGH14 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 2.5e-48
Identity = 100/127 (78.74%), Postives = 110/127 (86.61%), Query Frame = 1


Query: 15  SPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPSNL 74
           S SSS SIGKWKFDVFLSFRGEDTRGGFTDHLYKALT+KGISTFRDENEIEEGE I SNL
Sbjct: 4   SSSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIEEGEDISSNL 63

Query: 75  LASIDASRFAIVVVSEDYASSRWCLEELARMFECKK----EVLPIFYKVDPSHVKNQSGT 134
           L SI+ASRFAIVVVSE+YASSRWCLEEL ++FEC++    +VLPIFYKVDPSHV+NQ G 
Sbjct: 64  LDSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDPSHVRNQGGI 123

Query: 135 FEEAFVK 138
            +    K
Sbjct: 124 GKTTIAK 130

[Close]

BLAST of Csa5G648640 vs. TrEMBL
Match: Q5DMV0_CUCME (MRGH14 (Fragment) OS=Cucumis melo GN=MRGH14 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 9.8e-45
Identity = 94/113 (83.19%), Postives = 103/113 (91.15%), Query Frame = 1


Query: 13  SSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPS 72
           SSS SSS SIGKWKFDVFLSFRGEDTRGGFTDHLYKALT+KGISTFRDENEI+EGE I S
Sbjct: 4   SSSSSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIQEGEDISS 63

Query: 73  NLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKK----EVLPIFYKVDP 122
           NLL SI+ASRFAIVVVSE+YASSRWCLEEL ++FEC++    +VLPIFYKVDP
Sbjct: 64  NLLDSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDP 116

[Close]

BLAST of Csa5G648640 vs. TrEMBL
Match: J7FWP5_ROSRU (TIR-NBS-LRR OS=Rosa rugosa GN=ruRdr1C PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 8.3e-44
Identity = 90/164 (54.88%), Postives = 121/164 (73.78%), Query Frame = 1


Query: 10  AASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEH 69
           A S+   +SS S   WK+DVFLSFRGEDTR GFTD+LY  L R+GI TFRD+ ++E G  
Sbjct: 2   ALSTQVRASSGSAFPWKYDVFLSFRGEDTRKGFTDYLYHELQRRGIRTFRDDPQLERGTA 61

Query: 70  IPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKE---VLPIFYKVDPSHVKN 129
           I   LL +I+ SRFAIVV+S +YASS WCL EL+++ EC +E   +LPIFY+VDPSHV++
Sbjct: 62  ISPELLTAIEQSRFAIVVLSPNYASSTWCLLELSKILECMEERGTILPIFYEVDPSHVRH 121

Query: 130 QSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           Q G+F EAF +HE++FG G+ KV+ WR  LT++A+   W S+ +
Sbjct: 122 QRGSFAEAFQEHEEKFGVGNKKVEGWRDALTKVASLAGWTSKDY 165

[Close]

BLAST of Csa5G648640 vs. TrEMBL
Match: J7G2Z2_ROSRU (TIR-NBS-LRR OS=Rosa rugosa GN=ruRdr1I PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.4e-43
Identity = 89/164 (54.27%), Postives = 122/164 (74.39%), Query Frame = 1


Query: 10  AASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEH 69
           A S+   +SS S  +WK+DVFLSFRGEDTR GFTD+LY  L R+GI TFRD+ ++E G  
Sbjct: 2   ALSTQVRASSSSALQWKYDVFLSFRGEDTRKGFTDYLYIELQRQGIRTFRDDPQLERGTA 61

Query: 70  IPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKE---VLPIFYKVDPSHVKN 129
           I   LL +I+ SRFAIVV+S  YA+S WCL EL+++ EC +E   +LPIFY+VDPSHV++
Sbjct: 62  ISPELLTAIEQSRFAIVVLSPKYATSTWCLRELSKILECMEERGTILPIFYEVDPSHVRH 121

Query: 130 QSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           Q G+F EAF +HE++FG G+ +V+ WR  LT++A+   W S+S+
Sbjct: 122 QRGSFAEAFQEHEEKFGVGNKEVEGWRDALTKVASLAGWTSESY 165

[Close]

BLAST of Csa5G648640 vs. TAIR10
Match: AT1G72890.2 (AT1G72890.2 Disease resistance protein (TIR-NBS class))

HSP 1 Score: 152.5 bits (384), Expect = 2.3e-37
Identity = 84/168 (50.00%), Postives = 106/168 (63.10%), Query Frame = 1


Query: 7   SSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEE 66
           S S +SSSS S +P     KFDVFLSFRG+DTR  F   LYKAL  KGI TF+D+ E+E 
Sbjct: 23  SRSLSSSSSSSFTPQ----KFDVFLSFRGKDTRKNFVSFLYKALVSKGIRTFKDDEELER 82

Query: 67  GEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKK----EVLPIFYKVDPS 126
           G  IP  L  +I  SR A+VVVS  Y +S WCLEEL  + + +K     V+PIFY+++PS
Sbjct: 83  GRPIPPELRQAIKGSRIAVVVVSVTYPASSWCLEELREILKLEKLGLLTVIPIFYEINPS 142

Query: 127 HVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
            V+ QSG   + F KHEKR  R   +V+SWR  LT+LA+     S++W
Sbjct: 143 DVRRQSGVVSKQFKKHEKRQSR--ERVKSWREALTKLASLSGECSKNW 184

[Close]

BLAST of Csa5G648640 vs. TAIR10
Match: AT1G72950.1 (AT1G72950.1 Disease resistance protein (TIR-NBS class))

HSP 1 Score: 141.7 bits (356), Expect = 4.1e-34
Identity = 77/150 (51.33%), Postives = 94/150 (62.67%), Query Frame = 1


Query: 26  KFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPSNLLASIDASRFAI 85
           KFDVFLSFRG DTR  F   LYK L R+ I TF+D+ E++ G  I   L+ +I+ SRFA+
Sbjct: 10  KFDVFLSFRGLDTRRSFISFLYKELIRRNIRTFKDDKELKNGRRITPELIRAIEGSRFAV 69

Query: 86  VVVSEDYASSRWCLEELARMFECKK----EVLPIFYKVDPSHVKNQSGTFEEAFVKHEKR 145
           VVVS +YA+SRWCLEEL ++ + +     +V+PIFY VDP HV+ Q G   E F KHE  
Sbjct: 70  VVVSVNYAASRWCLEELVKIMDFENMGSLKVMPIFYGVDPCHVRRQIGEVAEQFKKHE-- 129

Query: 146 FGRGD-GKVQSWRTFLTELANTKAWLSQSW 171
            GR D  KV SWR  LT LA+     S  W
Sbjct: 130 -GREDHEKVLSWRQALTNLASISGDCSWKW 156

[Close]

BLAST of Csa5G648640 vs. TAIR10
Match: AT5G17680.1 (AT5G17680.1 disease resistance protein (TIR-NBS-LRR class), putative)

HSP 1 Score: 138.3 bits (347), Expect = 4.5e-33
Identity = 74/161 (45.96%), Postives = 97/161 (60.25%), Query Frame = 1


Query: 11  ASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHI 70
           AS  S SSS S   WK DVF+SFRGED R  F  HL+    R GI  FRD+ +++ G+ I
Sbjct: 2   ASLPSSSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSI 61

Query: 71  PSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKE-VLPIFYKVDPSHVKNQSG 130
              L+ +I  SRFAIVVVS +YA+S WCL+EL ++ EC K+ ++PIFY+VDPS V+ Q G
Sbjct: 62  SPELIDAIKGSRFAIVVVSRNYAASSWCLDELLKIMECNKDTIVPIFYEVDPSDVRRQRG 121

Query: 131 TFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           +F E    H  +      KV  W+  L +LA      S++W
Sbjct: 122 SFGEDVESHSDK-----EKVGKWKEALKKLAAISGEDSRNW 157

[Close]

BLAST of Csa5G648640 vs. TAIR10
Match: AT1G65850.2 (AT1G65850.2 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 137.5 bits (345), Expect = 7.7e-33
Identity = 75/167 (44.91%), Postives = 100/167 (59.88%), Query Frame = 1


Query: 8   SSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEG 67
           SS  SSSS  SS S  KW   VF SFRGED R GF  H++K   RKGI+ F D NEI+ G
Sbjct: 29  SSTISSSSVPSSSSSHKWTHQVFPSFRGEDVRRGFLSHIHKEFQRKGITPFID-NEIKRG 88

Query: 68  EHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKE----VLPIFYKVDPSH 127
           E I   ++ +I  S+ AIV++S +YASS WCL+EL  + +CK+E    V+PIFY+VDPS 
Sbjct: 89  ESIGLEIIHAIRESKIAIVLLSRNYASSSWCLDELVEIMKCKEEFSQIVIPIFYRVDPSD 148

Query: 128 VKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           VK  +G F   F       G+ +  ++ WR  L ++  T  + S++W
Sbjct: 149 VKKLTGNFGNVF--KNNCVGKTNEVIRKWRQALAKMGTTTGYDSRNW 192

[Close]

BLAST of Csa5G648640 vs. TAIR10
Match: AT1G72910.1 (AT1G72910.1 Toll-Interleukin-Resistance (TIR) domain-containing protein)

HSP 1 Score: 135.2 bits (339), Expect = 3.8e-32
Identity = 70/139 (50.36%), Postives = 88/139 (63.31%), Query Frame = 1


Query: 26  KFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPSNLLASIDASRFAI 85
           K+DVFLSFRG DTR  F   LYK L R+ I TF+D+ E+E G+ I S L  +I+ SRFA+
Sbjct: 8   KYDVFLSFRGHDTRQNFISFLYKELVRRSIRTFKDDKELENGQRISSELKRTIEVSRFAV 67

Query: 86  VVVSEDYASSRWCLEELARMFECKKE----VLPIFYKVDPSHVKNQSGTFEEAFVKHEKR 145
           VVVSE YA+S WCL+EL  + + +K+    V+PIFY V+P+HV+ Q+G   E F KH  R
Sbjct: 68  VVVSETYAASSWCLDELVTIMDFEKKGSITVMPIFYGVEPNHVRWQTGVLAEQFKKHGSR 127

Query: 146 FGRGDGKVQSWRTFLTELA 161
                 KV  WR  LT  A
Sbjct: 128 --EDHEKVLKWRQALTNFA 144

[Close]

BLAST of Csa5G648640 vs. NCBI nr
Match: gi|778707836|ref|XP_011656070.1| (PREDICTED: TMV resistance protein N-like isoform X1 [Cucumis sativus])

HSP 1 Score: 347.1 bits (889), Expect = 1.8e-92
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 1


Query: 1   MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD 60
           MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD
Sbjct: 1   MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD 60

Query: 61  ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD 120
           ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD
Sbjct: 61  ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD 120

Query: 121 PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW
Sbjct: 121 PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 170

[Close]

BLAST of Csa5G648640 vs. NCBI nr
Match: gi|700197466|gb|KGN52643.1| (MRGH14 [Cucumis sativus])

HSP 1 Score: 347.1 bits (889), Expect = 1.8e-92
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 1


Query: 1   MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD 60
           MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD
Sbjct: 1   MVPSSPSSSAASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRD 60

Query: 61  ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD 120
           ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD
Sbjct: 61  ENEIEEGEHIPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKEVLPIFYKVD 120

Query: 121 PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW
Sbjct: 121 PSHVKNQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 170

[Close]

BLAST of Csa5G648640 vs. NCBI nr
Match: gi|659119242|ref|XP_008459550.1| (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 266.2 bits (679), Expect = 4.1e-68
Identity = 132/162 (81.48%), Postives = 143/162 (88.27%), Query Frame = 1


Query: 13  SSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPS 72
           SSS SSS SIGKWKFDVFLSFRGEDTRGGFTDHLYKALT+KGISTFRDENEI+EGE I S
Sbjct: 4   SSSSSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIQEGEDISS 63

Query: 73  NLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKK----EVLPIFYKVDPSHVKNQS 132
           NLL SI+ASRFAIVVVSE+YASSRWCLEEL ++FEC++    +VLPIFYKVDPSHV+NQ 
Sbjct: 64  NLLDSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDPSHVRNQR 123

Query: 133 GTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSW 171
           G FEEAFVKHE RFGR D KVQ WR  LT+LAN KAWLSQSW
Sbjct: 124 GRFEEAFVKHEMRFGRDDIKVQKWRRLLTKLANLKAWLSQSW 165

[Close]

BLAST of Csa5G648640 vs. NCBI nr
Match: gi|469474101|gb|AGH33842.1| (RGH14 [Cucumis melo])

HSP 1 Score: 199.9 bits (507), Expect = 3.6e-48
Identity = 100/127 (78.74%), Postives = 110/127 (86.61%), Query Frame = 1


Query: 15  SPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPSNL 74
           S SSS SIGKWKFDVFLSFRGEDTRGGFTDHLYKALT+KGISTFRDENEIEEGE I SNL
Sbjct: 4   SSSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIEEGEDISSNL 63

Query: 75  LASIDASRFAIVVVSEDYASSRWCLEELARMFECKK----EVLPIFYKVDPSHVKNQSGT 134
           L SI+ASRFAIVVVSE+YASSRWCLEEL ++FEC++    +VLPIFYKVDPSHV+NQ G 
Sbjct: 64  LDSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDPSHVRNQGGI 123

Query: 135 FEEAFVK 138
            +    K
Sbjct: 124 GKTTIAK 130

[Close]

BLAST of Csa5G648640 vs. NCBI nr
Match: gi|51477403|gb|AAU04776.1| (MRGH14 [Cucumis melo])

HSP 1 Score: 188.0 bits (476), Expect = 1.4e-44
Identity = 94/113 (83.19%), Postives = 103/113 (91.15%), Query Frame = 1


Query: 13  SSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEHIPS 72
           SSS SSS SIGKWKFDVFLSFRGEDTRGGFTDHLYKALT+KGISTFRDENEI+EGE I S
Sbjct: 4   SSSSSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIQEGEDISS 63

Query: 73  NLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKK----EVLPIFYKVDP 122
           NLL SI+ASRFAIVVVSE+YASSRWCLEEL ++FEC++    +VLPIFYKVDP
Sbjct: 64  NLLDSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDP 116

The following BLAST results are available for this feature:

BLAST of Csa5G648640 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TMVRN_NICGU	2.3e-39	52.98	TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1	[more]
TIR_ARATH	3.4e-30	48.92	Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana GN=TIR PE=1 SV=...	[more]
Y4117_ARATH	4.9e-29	43.31	Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g111...	[more]
TAO1_ARATH	1.1e-28	44.31	Disease resistance protein TAO1 OS=Arabidopsis thaliana GN=TAO1 PE=3 SV=1	[more]
RPP1_ARATH	7.0e-28	41.67	Probable disease resistance protein RPP1 OS=Arabidopsis thaliana GN=RPP1 PE=2 SV...	[more]

BLAST of Csa5G648640 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KSU0_CUCSA	1.3e-92	100.00	MRGH14 OS=Cucumis sativus GN=Csa_5G648640 PE=4 SV=1	[more]
M4R4K0_CUCME	2.5e-48	78.74	RGH14 OS=Cucumis melo GN=RGH14 PE=4 SV=1	[more]
Q5DMV0_CUCME	9.8e-45	83.19	MRGH14 (Fragment) OS=Cucumis melo GN=MRGH14 PE=4 SV=1	[more]
J7FWP5_ROSRU	8.3e-44	54.88	TIR-NBS-LRR OS=Rosa rugosa GN=ruRdr1C PE=4 SV=1	[more]
J7G2Z2_ROSRU	1.4e-43	54.27	TIR-NBS-LRR OS=Rosa rugosa GN=ruRdr1I PE=4 SV=1	[more]

BLAST of Csa5G648640 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G72890.2	2.3e-37	50.00	Disease resistance protein (TIR-NBS class)	[more]
AT1G72950.1	4.1e-34	51.33	Disease resistance protein (TIR-NBS class)	[more]
AT5G17680.1	4.5e-33	45.96	disease resistance protein (TIR-NBS-LRR class), putative	[more]
AT1G65850.2	7.7e-33	44.91	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT1G72910.1	3.8e-32	50.36	Toll-Interleukin-Resistance (TIR) domain-containing protein	[more]

BLAST of Csa5G648640 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778707836\|ref\|XP_011656070.1\|	1.8e-92	100.00	PREDICTED: TMV resistance protein N-like isoform X1 [Cucumis sativus]	[more]
gi\|700197466\|gb\|KGN52643.1\|	1.8e-92	100.00	MRGH14 [Cucumis sativus]	[more]
gi\|659119242\|ref\|XP_008459550.1\|	4.1e-68	81.48	PREDICTED: TMV resistance protein N-like [Cucumis melo]	[more]
gi\|469474101\|gb\|AGH33842.1\|	3.6e-48	78.74	RGH14 [Cucumis melo]	[more]
gi\|51477403\|gb\|AAU04776.1\|	1.4e-44	83.19	MRGH14 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000157	TIR_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **Biological Process**
Term	Definition
GO:0007165	signal transduction

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0005515	protein binding

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU121813	cucumber EST collection version 3.0	transcribed_cluster
CU147337	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa5G648640.1	Csa5G648640.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU121813	CU121813	transcribed_cluster
CU147337	CU147337	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	GENE3D	G3DSA:3.40.50.10140		coord: 24..141 score: 2.1
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF01582	TIR	coord: 26..164 score: 5.6
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 26..162 score: 8.7
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROFILE	PS50104	TIR	coord: 25..162 score: 28
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	unknown	SSF52200	Toll/Interleukin receptor TIR domain	coord: 17..162 score: 3.53
None	No IPR available	PANTHER	PTHR11017	LEUCINE-RICH REPEAT-CONTAINING PROTEIN	coord: 7..161 score: 2.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa5G648640	Cla012439	Watermelon (97103) v1	cuwmB379
Csa5G648640	MELO3C022154	Melon (DHL92) v3.5.1	cumeB358
Csa5G648640	Lsi08G001160	Bottle gourd (USVL1VR-Ls)	culsiB391
Csa5G648640	MELO3C022154.2	Melon (DHL92) v3.6.1	cumedB337
Csa5G648640	Cla97C08G145530	Watermelon (97103) v2	cuwmbB403
Csa5G648640	Bhi06G000178	Wax gourd	cuwgoB461
Csa5G648640	CSPI05G30730	Wild cucumber (PI 183967)	cpicuB239
Csa5G648640	Cucsa.237390	Cucumber (Gy14) v1	cgycuB337
Csa5G648640	CsGy5G030080	Cucumber (Gy14) v2	cgybcuB223

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa5G648640	Wax gourd	cuwgoB468
Csa5G648640	Cucurbita maxima (Rimu)	cmacuB029
Csa5G648640	Cucurbita maxima (Rimu)	cmacuB483
Csa5G648640	Cucurbita moschata (Rifu)	cmocuB020
Csa5G648640	Cucurbita moschata (Rifu)	cmocuB472
Csa5G648640	Melon (DHL92) v3.5.1	cumeB368
Csa5G648640	Watermelon (Charleston Gray)	cuwcgB413
Csa5G648640	Cucurbita pepo (Zucchini)	cpecuB579
Csa5G648640	Cucurbita pepo (Zucchini)	cpecuB792
Csa5G648640	Bottle gourd (USVL1VR-Ls)	culsiB392
Csa5G648640	Melon (DHL92) v3.6.1	cumedB346
Csa5G648640	Silver-seed gourd	carcuB0183
Csa5G648640	Silver-seed gourd	carcuB0698